CN102960246B - Tissue culturing method for effectively improving general flavone content of tartary buckwheat - Google Patents
Tissue culturing method for effectively improving general flavone content of tartary buckwheat Download PDFInfo
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- CN102960246B CN102960246B CN201210473206.7A CN201210473206A CN102960246B CN 102960246 B CN102960246 B CN 102960246B CN 201210473206 A CN201210473206 A CN 201210473206A CN 102960246 B CN102960246 B CN 102960246B
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- 229930003944 flavone Natural products 0.000 title claims abstract description 35
- 235000011949 flavones Nutrition 0.000 title claims abstract description 35
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 26
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 26
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 244000130270 Fagopyrum tataricum Species 0.000 title claims abstract description 20
- 235000014693 Fagopyrum tataricum Nutrition 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000012258 culturing Methods 0.000 title abstract 6
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- 235000009419 Fagopyrum esculentum Nutrition 0.000 claims abstract description 28
- 239000002243 precursor Substances 0.000 claims abstract description 22
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 8
- 239000012138 yeast extract Substances 0.000 claims abstract description 8
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 26
- 238000005286 illumination Methods 0.000 claims description 16
- 239000000725 suspension Substances 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
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- 241000238631 Hexapoda Species 0.000 claims description 4
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- 230000004069 differentiation Effects 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 4
- 230000001939 inductive effect Effects 0.000 claims description 4
- 229960002523 mercuric chloride Drugs 0.000 claims description 4
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 4
- 239000002574 poison Substances 0.000 claims description 4
- 231100000614 poison Toxicity 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
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- 239000002028 Biomass Substances 0.000 abstract description 15
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 abstract description 14
- 239000001632 sodium acetate Substances 0.000 abstract description 14
- 235000017281 sodium acetate Nutrition 0.000 abstract description 14
- 150000002213 flavones Chemical class 0.000 abstract description 9
- 238000005516 engineering process Methods 0.000 abstract description 4
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- FJVUSYSRMMZRPX-UHFFFAOYSA-N 2-acetyloxypropanedioic acid Chemical compound CC(=O)OC(C(O)=O)C(O)=O FJVUSYSRMMZRPX-UHFFFAOYSA-N 0.000 description 2
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- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a tissue culturing method for effectively improving a general flavone content of tartary buckwheat. The tissue culturing method uses a precursor feeding technology. A metabolic intermediate sodium acetate or yeast extract of a flavonoid matter is added in a buckwheat cell suspension culture, so that not only can the general flavone content in the buckwheat culture cell be obviously improved, but also the tissue culturing method has obvious acceleration effect on biomass multiplication of a tissue culture matter. According to the tissue culturing method provided by the invention, the foundation is laid for further developing a buckwheat plant secondary metabolite in a large scale, so that the rapid generation of effective components of the buckwheat plant is achieved; and meanwhile, the tissue culturing method can provide important technical support for further research on molecule conditioning of flavones secondary metabolism in the buckwheat plant.
Description
Technical field
The present invention relates to a kind of method for tissue culture of tartarian buckwheat, particularly relate to a kind of tissue culture method that effectively can improve tartarian buckwheat general flavone content.
Background technology
Buckwheat is a kind of plant of integration of drinking and medicinal herbs, has bitter buckwheat and sweet buckwheat two kinds.Owing to all containing a large amount of Flavonoid substances in the multiple organs such as its seed of tartarian buckwheat, root, stem, leaf and flower, be thus described as " hypoglycemic, hypotensive, reducing blood lipid " three fall food.Along with the continuous discovery of its nutritive value and medical value, tartarian buckwheat plant resources is more and more subject to the favor of people.But because tartarian buckwheat plant has autophilous feature, thus cause it to there is artificial hybridization in agricultural production breeding and be difficult to successfully, this is also the major reason that current tartarian buckwheat is difficult to obtain high yield improved seeds in breeding.Tissue cultures can solve the bitter problem of supporting wheat and running in breeding to a certain extent.At present existingly cultivate the report of its callus with tartarian buckwheat stem section, blade, petiole for explant.
Large quantity research shows, in the suspension incubation of plant cell, by adding the precursor of final purpose compound, greatly can improve the Secondary Metabolite Contents of suspended culture cell.Yet there are no and adopt precursor feeding technology to significantly improve the report of general flavone content in tartarian buckwheat group training thing.
Summary of the invention
The object of the present invention is to provide a kind of tissue culture method of effective raising tartarian buckwheat general flavone content, the method effectively improves general flavone content in buckwheat culture by adding specific precursor in the liquid culture of buckwheat cell.
For achieving the above object, the solution that the present invention adopts comprises the steps:
(1) process of explant: choose healthy growth and without the bitter buckwheat stem section of damage by disease and insect as explant, and disinfection;
(2) Fiber differentiation of callus: cancel the explant after poison, the length of 0.5cm ~ 1.5cm is cut into after blotting the moisture on surface, then callus inducing medium MS+6-BA 0.1 ~ 2.0mgL+2 is accessed, 4-D 1 ~ 6mgL+IAA 0 ~ 1mgL+ sucrose 20 ~ 60gL
-1+ agar 5.0 ~ 7.0gL
-1in cultivate, condition of culture is pH value 5.5 ~ 6.8, illumination every day 8 ~ 16 hours, intensity of illumination 1000 ~ 2000lx, cultivation temperature 18 ~ 28 DEG C;
(3) suspension of callus is cultivated: cut the growth callus of 15 ~ 25 days, with 10 ~ 50gL
-1inoculum concentration be linked into the liquid nutrient medium MS+6-BA1.0 ~ 2.0mgL being added with precursor feeding thing
-1+ NAA0.1 ~ 0.5mgL
-1+ sucrose 20 ~ 60gL
-1+ Vc 100 ~ 300mgL
-1in carry out suspension cultivate, condition of culture is pH value 5.5 ~ 6.5, cultivation temperature 18 ~ 24 DEG C, illumination every day 8 ~ 12 hours, intensity of illumination 600 ~ 1000lx, shaking speed is 120 ~ 140r/min, described precursor feeding thing is sodium acetate or yeast extract, the addition of sodium acetate is 1 ~ 10mgL, and the addition of yeast extract is 0.5 ~ 5gL.
Callus cell after above-mentioned suspension is cultivated 25 days leaches from culture fluid, after wiping dry substance surface moisture, takes weight, is fresh weight with dry filter paper; Biomass proliferation times=(harvest yield fresh weight-inoculum concentration fresh weight)/inoculum concentration fresh weight.
The baking oven that the buckwheat callus cell of above-mentioned cultivation is placed in 55 DEG C is dried to constant weight, and by spectrophotometric method, flavones content in buckwheat group training thing is detected, working sample by directly measuring absorbance at 500nm place after colour developing, and calculates the content of flavones in group training thing.
Disinfect described in above-mentioned steps (1) refer to bitter buckwheat stem section concentration be 0.1% mercuric chloride sterilization 3 ~ 8 minutes, then use sterile water wash 3 ~ 5 times.
Bitter buckwheat stem section in above-mentioned steps (1) selects the tender stem segments near tartarian buckwheat plant terminal bud end.
Callus growth selection in above-mentioned steps (3) is vigorous, quality is comparatively loose and callus that is that produce without brown stain.
The present invention has such thinking: the biosynthesis due to flavones comprises acetoxymalonic acid approach and shikimic acid pathway, and the A ring of flavones is acetic acid synthesizes through acetoxymalonic acid approach, therefore, feed and raise the direct precursor that precursor sodium acetate is synthesis flavones, thus add the sodium acetate of suitable concentration, the synthesis of secondary metabolite flavones in buckwheat suspended culture cell can be improved significantly.And the precursor yeast extract of feeding, as a kind of biological derivant by a series of key enzymes in activation phenylpropyl alcohol alkanes approach, as phenylalnine ammonialyase (PAL), cinnamic acid 4-hydroxylase (CA4H) and coumaric acid-CoA connect the accumulation that enzyme (4CL) etc. can impel flavonoids.
Therefore the present invention adopts precursor feeding technology, by in the suspending nutrient solution of tartarian buckwheat stem section cell, the metabolic intermediate of material such as class such as synthesis such as flavones such as interpolation sodium acetate or yeast extract etc. effectively can improve the general flavone content in tartarian buckwheat cultured cell, for the production carrying out plant buckwheat secondary metabolite further is on a large scale laid a good foundation, the molecular regulation also for studying flavones secondary metabolism in plant buckwheat further provides important technical support.
Embodiment
Embodiment 1
(1) process of explant: choose healthy growth and without 1st ~ 2 tender stem segmentses of the close bitter buckwheat plant terminal bud end of damage by disease and insect as explant, sterilize 4 minutes with the mercuric chloride that concentration is 0.1%, then use sterile water wash 4 times;
(2) Fiber differentiation of callus: cancel the explant after poison, is cut into the length of 0.6cm after blotting the moisture on surface, then access callus inducing medium MS+6-BA 1.0mgL+2,4-D5mgL+IAA 0.5mgL+ sucrose 35gL
-1+ agar 6.0gL
-1in cultivate, condition of culture is: pH value 6.0, illumination every day 12 hours, intensity of illumination 1500lx, cultivation temperature 25 DEG C, and cultivate 15 days statistics, the inductivity of callus is 100%;
(3) suspension of callus is cultivated: cut that the growth growth of 20 days is vigorous, the comparatively loose and callus that is that produce without brown stain of quality, with 30gL
-1inoculum concentration be linked into the liquid nutrient medium MS+6-BA1.0mgL being added with sodium acetate
-1+ NAA0.2mgL
-1+ sucrose 25gL
-1+ Vc 200mgL
-1in carry out suspension cultivate, condition of culture is pH value 6.0, cultivation temperature 22 DEG C, illumination every day 10 hours, intensity of illumination 800lx, and shaking speed is 130r/min, and the addition of described sodium acetate is 5mg/L, namely adds 5mg sodium acetate in often liter of medium;
(4) calculating of culture biomass proliferation times: carrying out suspension cultivation after 25 days, callus cell in culture fluid is leached from culture fluid, after wiping dry substance surface moisture with dry filter paper, take weight, and the biomass proliferation times calculating buckwheat cell is 2.71, exceed 33.50% than the control group biomass proliferation times 2.03 not adding any precursor feeding thing;
(5) mensuration of general flavone content in culture: the baking oven that the buckwheat histocyte of cultivation is placed in 55 DEG C is dried to constant weight, and measure absorbance by spectrophotometric method at 500nm place, the general flavone content calculated further in culture is 5.04%, has exceeded 52.73% than the control group general flavone content 3.30% not adding any precursor feeding thing.
Embodiment 2
(1) process of explant: choose healthy growth and without 1 ~ 2 bitter buckwheat stem section of the close bitter buckwheat plant terminal bud end of damage by disease and insect as explant, sterilize 6 minutes with the mercuric chloride that concentration is 0.1%, then use sterile water wash 3 times;
(2) Fiber differentiation of callus: cancel the explant after poison, is cut into the length of 1.0cm after blotting the moisture on surface, then access callus inducing medium MS+6-BA 0.5mgL+2,4-D2mgL+ sucrose 50gL
-1+ agar 6.0gL
-1in cultivate, condition of culture is: pH value 5.8, illumination every day 10 hours, intensity of illumination 1200lx, cultivation temperature 25 DEG C;
(3) suspension of callus is cultivated: cut the growth callus of 24 days, with 40gL
-1inoculum concentration be linked into the liquid nutrient medium MS+6-BA1.5mgL being added with sodium acetate
-1+ NAA0.4mgL
-1+ sucrose 30gL
-1+ Vc 150mgL
-1in carry out suspension cultivate, condition of culture is pH value 6.0, cultivation temperature 22 DEG C, illumination every day 10 hours, intensity of illumination 800lx, and shaking speed is 130r/min, and the addition of described sodium acetate is 5mg/L, namely adds 5mg sodium acetate in often liter of medium;
(4) calculating of culture biomass proliferation times: carrying out suspension cultivation after 25 days, callus cell in culture fluid is leached from culture fluid, after wiping dry substance surface moisture with dry filter paper, take weight, and the biomass proliferation times calculating buckwheat cell is 2.51;
(5) mensuration of general flavone content in culture: the baking oven that the buckwheat histocyte of cultivation is placed in 55 DEG C is dried to constant weight, and measure absorbance by spectrophotometric method at 500nm place, the general flavone content calculated further in culture is 4.84%.
Embodiment 3
Change the addition of the sodium acetate in embodiment 1 step (3) into 10mg/L, other step is with embodiment 1, the biomass proliferation times recording buckwheat cell in cultivation after 25 days is 2.15, has exceeded 5.91% than the control group biomass proliferation times 2.03 not adding any precursor feeding thing; And the general flavone content measured in culture is 5.46%, exceed 65.45% than the control group general flavone content 3.30% not adding any precursor feeding thing.Illustrate thus, in the liquid nutrient medium of buckwheat cell, when the sodium acetate precursor feeding substrate concentration added brings up to 10mg/L, buckwheat cellular biomass propagation is not increased significantly, but has facilitation significantly to the general flavone content improved in buckwheat cell.
Embodiment 4
Change the precursor feeding thing in embodiment 1 step (3) into yeast extract, addition is 2g/L, and other step is with embodiment 1.The biomass proliferation times recording buckwheat cell in cultivation after 25 days is 2.65, has exceeded 30.54% than the control group biomass proliferation times 2.03 not adding any precursor feeding thing; And the general flavone content recorded is 5.26%, exceed 59.39% than the control group general flavone content 3.30% not adding any precursor feeding thing.
Embodiment 5
The addition of the yeast extract in embodiment 3 step (3) changed into 5g/L, other step is with embodiment 3.The biomass proliferation times recording buckwheat cell in cultivation after 25 days is 2.09, has only exceeded 2.96% than the control group biomass proliferation times 2.03 not adding any precursor feeding thing; And the general flavone content recorded is 4.96%, exceeded 50.30% than the control group general flavone content 3.30% not adding any precursor feeding thing, compare can find out the present embodiment biomass proliferation times and general flavone content all lower than embodiment 3.
Claims (4)
1. effectively improve a tissue culture method for tartarian buckwheat general flavone content, it is characterized in that comprising the steps:
(1) process of explant: choose healthy growth and without the bitter buckwheat stem section of damage by disease and insect as explant, and disinfection;
(2) Fiber differentiation of callus: cancel the explant after poison, the length of 0.5cm ~ 1.5cm is cut into after blotting the moisture on surface, then callus inducing medium MS+6-BA 0.1 ~ 2.0mgL+2 is accessed, 4-D 1 ~ 6mgL+IAA 0 ~ 1mgL+ sucrose 20 ~ 60gL
-1+ agar 5.0 ~ 7.0gL
-1in cultivate, condition of culture is pH value 5.5 ~ 6.8, illumination every day 8 ~ 16 hours, intensity of illumination 1000 ~ 2000lx, cultivation temperature 18 ~ 28 DEG C;
(3) suspension of callus is cultivated: cut the growth callus of 15 ~ 25 days, with 10 ~ 50gL
-1inoculum concentration be linked into the liquid nutrient medium MS+6-BA1.0 ~ 2.0mgL being added with precursor feeding thing
-1+ NAA0.1 ~ 0.5mgL
-1+ sucrose 20 ~ 60gL
-1+ Vc 100 ~ 300mgL
-1in carry out suspension cultivate, condition of culture is pH value 5.5 ~ 6.5, cultivation temperature 18 ~ 24 DEG C, illumination every day 8 ~ 12 hours, intensity of illumination 600 ~ 1000lx, and shaking speed is 120 ~ 140r/min, described precursor feeding thing is yeast extract, and its addition is 0.5 ~ 5g/L.
2. the tissue culture method of a kind of effective raising tartarian buckwheat general flavone content according to claim 1, it is characterized in that: disinfect described in step (1) refer to bitter buckwheat stem section concentration be 0.1% mercuric chloride sterilization 3 ~ 8 minutes, then use sterile water wash 3 ~ 5 times.
3. the tissue culture method of a kind of effective raising tartarian buckwheat general flavone content according to claim 1, is characterized in that: the bitter buckwheat stem section in step (1) selects the tender stem segments near tartarian buckwheat plant terminal bud end.
4. the tissue culture method of a kind of effective raising tartarian buckwheat general flavone content according to claim 1, is characterized in that: callus described in step (3) is that growth is vigorous, quality is comparatively loose and callus that is that produce without brown stain.
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| CN105580735B (en) * | 2016-02-01 | 2021-10-22 | 珀莱雅化妆品股份有限公司 | Culture solution capable of improving resveratrol content in polygonum cuspidatum callus and culture method |
| CN108112474A (en) * | 2016-11-28 | 2018-06-05 | 山东农业大学 | A kind of method that cultured in vitro improves STEVIA REBAUDIANA RA contents |
| CN107548831A (en) * | 2017-08-01 | 2018-01-09 | 六安玫瑰红茶品有限公司 | A kind of mulberry tree management method for improving flavones in mulberry leaves content |
| CN107864860B (en) * | 2017-12-06 | 2019-02-12 | 广州今成生物科技有限公司 | A kind of cultural method improving sec-o-glucosylhamaudol content in windproof callus |
| CN116574668B (en) * | 2023-06-13 | 2023-12-05 | 浙江觅得优生物科技有限公司 | Method for promoting liquorice cells to release secondary metabolite liquorice total flavonoids into suspension culture medium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869073A (en) * | 2009-04-22 | 2010-10-27 | 中国科学院成都生物研究所 | Tartary buckwheat isolated regeneration culture method |
| CN102405833A (en) * | 2011-08-19 | 2012-04-11 | 西北大学 | Method for improving adventitious bud differentiation rate of tartary buck wheat through suspension culture |
| CN102499080A (en) * | 2011-10-23 | 2012-06-20 | 成都大学 | Plant fast propagating method using fagopyrum tataricum leaf stalks as explants |
Family Cites Families (2)
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| JP4917857B2 (en) * | 2006-09-27 | 2012-04-18 | 独立行政法人農業・食品産業技術総合研究機構 | Buckwheat sprout with high anthocyanin content |
| KR101174495B1 (en) * | 2010-04-13 | 2012-08-22 | 대한민국 | Cultivation method of pink-colored tatary buckwehat sprout with high anthocyanin and vitamin C |
-
2012
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869073A (en) * | 2009-04-22 | 2010-10-27 | 中国科学院成都生物研究所 | Tartary buckwheat isolated regeneration culture method |
| CN102405833A (en) * | 2011-08-19 | 2012-04-11 | 西北大学 | Method for improving adventitious bud differentiation rate of tartary buck wheat through suspension culture |
| CN102499080A (en) * | 2011-10-23 | 2012-06-20 | 成都大学 | Plant fast propagating method using fagopyrum tataricum leaf stalks as explants |
Non-Patent Citations (2)
| Title |
|---|
| Effects of Yeast Polysaccharide on Growth and Flavonoid Accumulation in Fagopyrum tataricum Sprout Cultures;Gang Zhao et al;《Molecules》;20120925(第17期);摘要、第11338页Figure 3 * |
| 利用悬浮细胞培养方法提高荞麦中总黄酮含量的研究;于寒松等;《食品科学》;20091231;第30卷(第22期);第38页左栏第1.3.1节、1.3.2节，摘要， * |
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