CN103509861B - Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines - Google Patents

Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines Download PDF

Info

Publication number
CN103509861B
CN103509861B CN201310350208.1A CN201310350208A CN103509861B CN 103509861 B CN103509861 B CN 103509861B CN 201310350208 A CN201310350208 A CN 201310350208A CN 103509861 B CN103509861 B CN 103509861B
Authority
CN
China
Prior art keywords
molecule marker
cotton
seq
segment
fineness
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201310350208.1A
Other languages
Chinese (zh)
Other versions
CN103509861A (en
Inventor
张天真
曹志斌
王鹏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Agricultural University
Original Assignee
Nanjing Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Agricultural University filed Critical Nanjing Agricultural University
Priority to CN201310350208.1A priority Critical patent/CN103509861B/en
Publication of CN103509861A publication Critical patent/CN103509861A/en
Application granted granted Critical
Publication of CN103509861B publication Critical patent/CN103509861B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention belongs to the field of molecular breeding, and relates to a molecular breeding method for synchronously improving the length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines. The method comprises the following steps: taking two introgression lines, namely IL080-D6-1 and 1047 (IL019-A2-6 chromosome segment introgression line), as the non-recurrent parents, respectively subjecting the non-recurrent parents to carry out hybridization with a recurrent parent (Xinjiang 0768) for one time, backcrossing two generations, in BC2F1 making strains containing different chromosome segments intercross with each other so as to obtain double-segment integrated F2 segregation population, identifying the double-segment homozygous strains through each two molecular labels on two segments, carrying out field tests under four circumstances, and keeping on selfing for two generations so as to obtain a double-segment homozygous novel cotton breed, whose fiber length, fiber strength and fineness have been prominently improved. A high-quality and high-yield novel cotton species, named "Nannong 08-66", has been cultured by utilizing the method provided by the invention.

Description

A kind of molecular breeding method synchronously being improved cotton fiber length, intensity and fineness by polymerization chromosome segment introgressive line
Technical field
The invention belongs to field of molecular breeding, relate to a kind of molecular breeding method synchronously being improved cotton fiber length, intensity and fineness by polymerization chromosome segment introgressive line.
Background technology
Cotton is global Important Economic crop.China is the big producing country of cotton, consumption big country and weaving big country.As Cotton Production big country, China's main product cotton region covers 16 provinces, city (autonomous region), plants cotton area 7000-8,000 ten thousand mu throughout the year, total produces about 6,000,000 tons, and account for that the world always produces 1/4th.Along with the progress of textile technology, textile product also develops into from coarse yarn the fine sand that high grade cotton yarn even reaches 100, and processing object also develops into Midlle-longstaple cotton, long stapled cotton and super long stapled cotton by general Medium Cotton.Wang Yanqin etc. chose 546 basic points by 2003-2007 years continuous 5 years in three large main product cotton regions, 2201 parts of cotton samples carry out fiber quality and detect the average 28.8mm of staple length finding China's upland cotton sample, luffing 24.0 ~ 34.7mm, mainly be in medium staple (28.0 ~ 31.0mm) scope of medium staple cotton, with long scope namely; The average 28.3cN/tex of strength, luffing 19.7-38.3cN/tex, majority is in medium (26.0 ~ 29.0cN/tex) scope, to strong scope namely; Mic value average 4.2, luffing 2.0 ~ 5.2, most at (3.7 ~ 4.9) scope (Wang Yanqin, Yang Weihua, Xu Hongxia, Zhou great Yun, Feng Xinai, Kuang Meng. the subject matter existed in China's Cotton Production and suggestion. Chinese agronomy circular, 2009,25 (14): 86-90).Cotton can meet the needs of textile industry substantially, but lacks length at more than 31mm, and strength is at more than 34cN/tex, and the extra best best kind of mic value between 3.7 ~ 4.2, meets the needs spinning more than 60 high grade cotton yarns.
The genetic analysis of general cotton variety fiber quality characteristics all shows typical amounts character inheritance mode, is subject to environmental influence.In different segregating population, additivity, dominant or epistatic analysis all may be occupied an leading position.Utilizing the high-tenacity proterties of wild species and the fine quality gene recombination of sea island cotton gradually to blend transformation, is the main path that contemporary breeding man obtains high tenacity fibre.Sea island cotton is long for breeding time, and in ripe evening, bell is little, and output is lower than upland cotton, but fiber finer is strong, is high-count yarn spun raw material; And the output of upland cotton is high, wide adaptability, fibrous quality is medium, but is significantly better than Asiatic cotton and cotton.The F that upland cotton, both sea island cotton hybridize 1can educate, phenotype is normal, and has the hybrid vigour of obvious output, quality, and successful incubation goes to sea, land cross-fertilize seed spread in production utilizes for India, Israel, China.But the violent segregation of the typical species hybridization of Posterity phenotype of both hybridization, cotton breeding man is out for cultivating both has a high yield of upland cotton, has again the new variety of sea island cotton high-quality, never success always.The application of molecular marking technique in fibrous quality improvement breeding greatly reduces the blindness selected in breeding process, realizes external source elite germplasm fast and permeates to Cultivar, widened the hereditary basis of kind.Domestic and international researchist has carried out a large amount of QTL location to fibrous quality, have for marker assisted selection breeding practice.
Chromosome segment introgressive line (Chromosome segment introgression lines, CSIL) be utilize hybridization, backcross and the whole genomic a series of near isogenic line of nurse crop that molecular marker assisted selection (marker assisted selection, MAS) builds.A chromosome segment isozygotied only from donor parents in its genome, and genomic rest part is identical with recurrent parent.It carries out genome research, particularly the ideal material of QTL location and Molecular design breeding.Wan Jianmin has utilized the CSSL population built by japonica rice Asominori (recurrent parent) and long-grained nonglutinous rice IR24 (nonrecurrent parent) to carry out the practice of molecular breeding.
Summary of the invention
The object of the invention is the above-mentioned deficiency for prior art, a kind of molecular breeding method improveing cotton fiber length, fibre strength and mic value is provided.
Object of the present invention realizes by following technical scheme:
Synchronously improved a molecular breeding method for cotton fiber length, intensity and fineness by polymerization chromosome segment introgressive line, comprising:
1) with Xinjiang cotton strain 0768 for recurrent parent (♀), with IL080 D6 1 (ZL201110127252.7) and 1047 (IL019 A2 6 chromosome segment introgressive lines) for nonrecurrent parent (♂), hybridized for 1 generation, in 2 generations that backcrossed, obtain BC 2f 1, per generation is all mixed receipts miscegenation;
2) molecule marker auxiliary first time is selected: BC 2f 1plantation, application CTAB method extracts DNA, adopt IL080 D6 molecule marker NAU3677 on 1 introgressed segment and NAU1454(table 1) and 1047 (IL019 A2 6 chromosome segment introgressive lines) introgressed segment on molecule marker NAU3419 and NAU2277(table 1) carry out pcr amplification, select to be respectively 180bp(NAU3677 containing 2 molecule marker bands simultaneously); Individual plant 300bp(NAU1454) be respectively 400bp(NAU3419 containing 2 molecule marker bands simultaneously); 120bp(NAU2277) individual plant is hybridized, and results cenospecies also plants the heterozygous plant continuation selfing obtaining 2 fragments polymerizations;
Table 1 primer characteristic strip size and sequence
The primer that note: NAU numbers develops (Han Z G by this laboratory from EST-SSR sequence; Guo W Z; Song X L; et al.Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum inallotetraploid cotton.Mol Gen Genomics; 2004,272:308-327; Han Z G, Wang C B, Song X L, etal.Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs inallotetraploid cotton.Theor Appl Genet, 2006,112:430-439; Wangzhen Guo, Caiping Cai, Changbiao Wang, et al.A preliminary analysis of genome structure and composition in Gossypiumhirsutum.BMC Genomics, 2008,9:314-331.). all primer information can from website www.cottonmarkers.orgupper download.
3) the auxiliary second time of molecule marker is selected: the F planting 2 fragment polymerizations 2segregating population, after DNA is extracted in every strain, recycling molecular marker primer pair NAU3677, NAU1454, NAU3419, NAU2277 determine F 2the genotype of individual plant, only has 4 molecule marker bands to be respectively 180bp simultaneously; 300bp; 400bp; The individual plant of 120bp, utilizes 62 polymorphism marks to carry out genetic background selection to these individual plants that isozygoty, individual plant that these are isozygotied proceed field test under the mirror of Fourth Ring and selfing 2 generation to F 2:4.Select F 2:4in a strain self propagated become family, carry out multispots trial.Its staple length 32.00 33.70mm, fibre strength 34.4 35.7cN/tex, mic value 3.01 3.45, Single boll weight 5.55 6.05g, ginning outturn 36.8 37.2%, therefore it is that we improve the improved line of cotton fiber length, fibre strength and mic value, called after " Nan Nong 08 66 ".
Beneficial effect:
A kind of molecular breeding method synchronously being improved cotton fiber length, intensity and fineness by polymerization chromosome segment introgressive line, overcome and alleviate serious Linkage drag that sea-land hybridization exists and, be conducive to the excellent genetic resources of fibrous quality in sea island cotton and import in upland cotton.Backcross and can recover the genetic background of recurrent parent, save the beneficial traits of nonrecurrent parent simultaneously, binding molecule marker assisted selection objective trait, rapidly and efficiently can cultivate all excellent New cotton line of staple length, fibre strength and mic value.
Provided by the present inventionly a kind ofly synchronously improve cotton fiber length by polymerization chromosome segment introgressive line, intensity has the following advantages with molecular breeding method tool compared with traditional pyramiding breeding technology that backcrosses of fineness: traditional pyramiding breeding technology that backcrosses exists the defect that crossing work amount is large, selects poor reliability, breeding cycle to grow.By molecular marker assisted selection objective trait, in 3-4, rapidly and efficiently can cultivate the New cotton line of the multiple target character such as high yield, high-quality.
The improved line " Nan Nong 08-66 " of improvement cotton fiber length, fibre strength and the mic value cultivated by the inventive method has following major advantage: with sea island cotton chromosome segment introgressive line IL080-D6-1 and 1047 (IL019-A2-6 chromosome segment introgressive line) for donor material, with 0768 for recurrent parent, output is obtained suitable with recurrent parent 0768 by molecular marker assisted selection, staple length and fibre strength significantly increase, the significantly reduced New cotton line of mic value " Nan Nong 08-66 ".This strain shows as staple length 32.00-33.70mm at Xinjiang stone river in 2012, fibre strength 34.4-35.7cN/tex, mic value 3.01-3.45, ginning outturn 36.8-37.2%, Single boll weight 5.55-6.10g is significantly high than the Improved lines only importing an IL080-D6-1 segment.
Accompanying drawing explanation
Fig. 1 synchronously improves cotton fiber length, intensity and fineness by polymerization chromosome segment introgressive line
Fig. 2 target interval linked marker NAU1454 is at polymerization biplate section F 2the individual plant that isozygotys is identified in colony
Note: M:DNA marker; P 1: sea 7124; P 2: 0768; F 1: (H7124x TM 1); 1 43:F 2individual plant arrow indication is the amplification difference position of SSR marker
Biological deposits:
IL080 D6 1, Classification And Nomenclature is upland cotton (Gossypium hirsutum.), within on 03 15th, 2011, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address, Institute of Microorganism, Academia Sinica, culture presevation number is CGMCC NO.4685.
1047, Classification And Nomenclature is upland cotton (Gossypium hirsutum.), and within 06 17th, 2013, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number is CGMCC NO.7754.
0768, Classification And Nomenclature is upland cotton (Gossypium hirsutum.), and within 05 06th, 2013, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number is CGMCC NO.7559.
South agriculture 08 66, Classification And Nomenclature is upland cotton (Gossypium hirsutum.), and within 05 06th, 2013, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number is CGMCC NO.7560.
Embodiment:
Embodiment 1
Selection parent: Agricultural University Of Nanjing's crop genetic and germplasm innovation National Key Laboratory Cotton Research Institute with upland cotton Genetic standard line TM 1 for recurrent parent, sea island cotton sea 7124 product that are disease-resistant, high-quality are donor parents (nonrecurrent parent), backcrossing, advanced lines combines the SSR molecular marker assisted Selection covering full-length genome, cultivated domestic first cover upland cotton Genetic standard line TM the sea island cotton chromosome segment introgressive line 174 of 1 background, its genomic fraction of coverage reaches 83.5%.Detected by the cotton fibre quality carried out sea island cotton chromosome segment introgressive line continuous 2 years, filter out staple length be significantly better than contrast introgressive line IL080 D6 1(CGMCC NO.4685) and 1047 (CGMCC NO.7754, for IL019 A2 6 chromosome segment introgressive lines).IL080 D6 1 staple length 31.5 32.5mm, fibre strength 32.7 33.5cN/tex, mic value 4.2 4.3, Single boll weight 6.8 7.2g, ginning outturn 36.8 38.8%, single basal munure 15 19, plant height 90 95cm.1047 (IL019 A2 6 chromosome segment introgressive lines) staple length 30.0 30.8mm, fibre strength 30.1 31.4cN/tex, mic value 4.5 4.6, Single boll weight 6.4 7.2g, ginning outturn 36.2 38.4%, single basal munure 14 17, plant height 90 95cm
Xinjiang cotton strain 0768(CGMCC NO.7559) be high yield, the disease-resistant cotton strain that suitable Xinjiang is planted.This strain staple length 30.14 30.43mm, fibre strength 35.3 35.7cN/tex, mic value 4.43 4.70, Single boll weight 5.42 5.72g, ginning outturn 38.8 40.5%, single basal munure 7 11, plant height 85 90cm.
Breed breeding: winter in 2007 is maternal at Sanya, Hainan with Xinjiang strain 0768, IL080 D6 1 and 1047 (IL019 A2 6 chromosome segment introgressive lines) be respectively the hybridization F that male parent prepares 2 combinations 1.Summer in 2008 plants a line 0768 and F respectively in Jiangpu testing station of Agricultural University Of Nanjing 1, with 0768 for male parent, the F of 2 combinations 1for maternal preparing hybrid produces BC 1f 1, all mix after ripe and receive.Winter in 2008 plants a line 0768 and BC respectively at Sanya, Hainan 1f 1, with 0768 for male parent, F 1for maternal preparing hybrid produces BC 2f 1, all mix after ripe and receive.Field management is carried out according to a conventional method.
Molecular marker assisted selection: summer in 2009 plants 2 row BC in Jiangpu testing station of Agricultural University Of Nanjing 2f 1, often row 10 12 strains, every strain gather 3 4 blades do not launched put into the eppendorf pipe of 1.5ml, and add the freshly prepared Extraction buffer 600 μ l of precooling, electric drill grinds, application CTAB method (Paterson AH, Brubaker CL, Wendel JF.A rapid methodfor extraction of cotton(Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis [J] .PlantMol Biol Rep, 1993, 11, 2:122 127) extract DNA, adopt IL080 D6 molecule marker NAU3419 and NAU2277 on molecule marker NAU3677 and NAU1454 and 1047 on 1 introgressed segment (IL019 A2 6 chromosome segment introgressive lines) introgressed segment carry out pcr amplification, amplification system 10 μ l, DNA profiling 1 μ l, 94 DEG C of denaturation 5min, 94 DEG C of sex change 0.5min, 57 DEG C of renaturation 0.5min, 72 DEG C extend 1min, after 30 circulations, 72 DEG C extend 10min again, amplified production is through native polyacrylamide gel electrophoresis: gel strength is 8%, electrophoretic buffer is 0.5 times of TBE, 170V constant voltage electrophoresis 1.5 hours.The primer of four molecule markers is in table 1.
Molecule marker auxiliary first time is selected: the BC utilizing target area close linkage mark NAU3677, NAU1454, NAU3419 and NAU2277 primer screening tool target fragment 2f 1individual plant, winter in 2009, plantation handed over F mutually in Sanya 1each 30 strains of colony, selfing results F 2seed.Utilize simultaneously 330 genomic SSR marker of uniform fold to IL080 D6 the donor kind TM-1 and 0768 of 1 and 1047 these two chromosome segment introgressive lines carry out polymorphism screening, obtain 31 pairs of polymorphism marks altogether to the BC of tool target fragment 2f 1individual plant carries out Analysis of Genetic Background, individual plant A and B picking out background recovery rate more than 80% respectively from 2 combinations is hybridized, wherein individual plant A from combination A(0768 × IL080 D6 1) background recovery rate is 80.6%, individual plant B is from combination B(0768 × 1047 (IL019 A2 6 chromosome segment introgressive lines)) background recovery rate is 83.9%.
The auxiliary second time of molecule marker is selected: the F extracting polymerization biplate section in summer in 2010 2colony 200 individual plant DNA, with linked marker NAU3677, NAU1454, NAU3419 and NAU2277(table 1 in two target fragment) analyze the F of biplate section polymerization 2identify 12 tool single slice A (NAU3677 NAU1454), 10 tool single slice B (NAU3419 NAU2277) and 8 biplate section target gene types in colony respectively to isozygoty individual plant (NAU3677 NAU1454 and NAU3419 NAU2277).Simultaneously based on this land, sea, laboratory collection of illustrative plates, on the mark density basis of context analyzer, mark is increased further original, a SSR marker is selected every 5 ~ 10cM, have selected 630 SSR marker altogether and polymorphism analysis (table 3) is carried out to parents, obtain 62 polymorphism marks (table 4) altogether, with these polymorphism marks, detection is carried out to the genetic background of the individual plant that isozygotys and find that the scope of genetic background recovery rate is between 60% ~ 88.8%, wherein biplate section isozygoty polymerization individual plant D1 genetic background in have 8 SSR marker sites to be shown as TM-1 genotype, background recovery rate is 87.1%.The F2 individual plant that simultaneously continues biplate section target gene type to isozygoty continues to carry out multiple environments testing in Nanjing and Shihezi of Xinjiang.By to Nanjing (2010), Nanjing (2011), the fiber check and measure result under four environment such as Nanjing (2012) and Shihezi (2012) and output analysis of survey results find biplate section isozygoty the staple length of polymerized strain D1, fibre strength and the extremely significantly super contrast of fineness improved effect and yield traits with contrast without significant difference (table 2).We are by this high-quality, high-yield cotton new lines called after " Nan Nong 08-66 " (CGMCC NO.7560).
Field test: 2010, plant F respectively in Jiangpu, Nanjing testing station summer in 2011 and 2012 2, F 2:3and F 2:4family, adopts the method for nutrition bowl seedling transplanting, and row length is 5 meters, wide 0.8 meter, and often row plants 12 strains, randomized complete-block design, single file district, often row 12 15 strains, twice repetition.Summer in 2012 plants BC in Shihezi of Xinjiang 2f 2:4family is tested, and plot experiment is that Liang Hang community is by 0.55(0.4+0.35+0.4) the wide film setting of m, row length is 5 meters, spacing in the rows 0.12 meter, randomized complete-block design, repeats for 2 times.
After after maturation, mixed cotton receiving is spent by row, often row gets 12 grams of gined cotton samples, repeats 2 times, send Henan cotton quality inspection center to detect gined cotton quality.Within 2012, improve the BC of cotton fiber length, fibre strength and mic value under Shihezi of Xinjiang's environment 2f 2:4middle selection plantation staple length 32.00 33.70mm, fibre strength 34.4 35.7cN/tex, mic value 3.01 3.45, Single boll weight 5.55 6.10g, the family of ginning outturn 36.8 37.2%, is improved line " Nan Nong 08-66 " (CGMCC NO.7560).This strain staple length and fibre strength significantly increase compared with 0768, and mic value significantly reduces compared with 0768, and output is suitable with recurrent parent 0768.
The southern agriculture of table 2 08 66 and the fibrous quality of contrast and the performance of yield factor under 4 environment
# field test: Nanjing (2010), Nanjing (2011), Nanjing (2012) and Shihezi (2012) are named as 1,2 respectively, 3 and 4*, * * be illustrated respectively in 0.05, there is significant difference in 0.01 level
Table 3 is for the molecule marker title of Foreground selection and characteristic strip size
The mark title of tool polymorphism and characteristic strip size between table 40768 and TM 1

Claims (1)

1. synchronously improved a molecular breeding method for cotton fiber length, fibre strength and mic value by polymerization chromosome segment introgressive line, it is characterized in that comprising:
1) be that recurrent parent is maternal with Xinjiang cotton strain 0768, be nonrecurrent parent male parent with IL080-D6-1 and 1047, hybridize 1 generation hybridization generation BC 1f 1, with 0768 for male parent, BC 1f 1for maternal preparing hybrid produces BC 2f 1, per generation is all mixed receipts miscegenation; Described strain 0768 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number is CGMCC NO.7559, described IL080-D6-1 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation number is CGMCC NO.4685, described strain 1047 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and culture presevation number is CGMCC NO.7754;
2) molecule marker auxiliary first time is selected: BC 2f 1plantation, application CTAB method extracts DNA, molecule marker NAU3677 and NAU1454 on IL080-D6-1 introgressed segment and molecule marker NAU3419 and NAU2277 on 1047 introgressed segments is adopted to carry out pcr amplification, the individual plant selecting simultaneously to be respectively NAU3677 and NAU1454 containing 2 molecule marker bands be respectively NAU3419 and NAU2277 individual plant containing 2 molecule marker bands simultaneously and hybridize, results cenospecies the heterozygous plant that plantation obtains 2 fragments polymerizations continues selfing; Wherein said molecule marker NAU3677 forward primer is SEQ ID NO.1, and reverse primer is SEQ ID NO.2, and PCR primer size is 180bp; Described molecule marker NAU1454 forward primer is SEQ ID NO.3, and reverse primer is SEQ ID NO.4, and PCR primer size is 300bp; Described molecule marker NAU3419 forward primer is SEQ ID NO.5, and reverse primer is SEQ ID NO.6, and PCR primer size is 400bp; Described molecule marker NAU2277 forward primer is SEQ ID NO.7, and reverse primer is SEQ ID NO.8, and PCR primer size is 120bp;
3) the auxiliary second time of molecule marker is selected: plant 2 fragment polymerization F 2segregating population, after DNA is extracted in every strain, recycling molecule marker NAU3677 and NAU1454 and molecule marker NAU3419 and NAU2277 carries out foreground selection and determines F 2the genotype of individual plant, the individual plant simultaneously only having 4 molecule marker bands to be respectively 180bp, 300bp, 400bp, 120bp utilizes the polymorphism mark of 61 shown in table 4 to carry out genetic background selection to these individual plants that isozygoty, individual plant that these are isozygotied proceed field test under Nanjing and Shihezi of Xinjiang four environment and selfing 2 generation to F 2:4, at F 2:4staple length 32.00-33.70mm is selected in family, fibre strength 34.4-35.7cN/tex, the fibrous quality of mic value 3.01-3.45, Single boll weight 5.55-6.05 g, ginning outturn 36.8-37.2% significantly improves the improved line that family is improvement cotton fiber length, fibre strength and mic value.
CN201310350208.1A 2013-08-12 2013-08-12 Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines Active CN103509861B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310350208.1A CN103509861B (en) 2013-08-12 2013-08-12 Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310350208.1A CN103509861B (en) 2013-08-12 2013-08-12 Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines

Publications (2)

Publication Number Publication Date
CN103509861A CN103509861A (en) 2014-01-15
CN103509861B true CN103509861B (en) 2015-05-13

Family

ID=49893292

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310350208.1A Active CN103509861B (en) 2013-08-12 2013-08-12 Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines

Country Status (1)

Country Link
CN (1) CN103509861B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109997682B (en) * 2019-04-25 2021-02-26 中国农业科学院棉花研究所 Breeding method for hybrid polymerized cotton with high-quality characters

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102229982B (en) * 2011-05-17 2012-11-07 南京农业大学 Molecular breeding method for improving cotton fiber length, fiber strength, and micronaire value
CN102229979B (en) * 2011-05-17 2012-11-14 南京农业大学 Molecular breeding method capable of improving length of cotton fibers by using gossypium barbadense chromosome segment introgression line
CN102229980B (en) * 2011-05-17 2013-01-02 南京农业大学 Molecular breeding method for improving micronaire value of cotton by using chromosome segment introgression lines of island cotton
CN102229981B (en) * 2011-05-17 2013-04-10 南京农业大学 Molecular breeding method for improving strength and micronaire value of cotton fibers

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102229982B (en) * 2011-05-17 2012-11-07 南京农业大学 Molecular breeding method for improving cotton fiber length, fiber strength, and micronaire value
CN102229979B (en) * 2011-05-17 2012-11-14 南京农业大学 Molecular breeding method capable of improving length of cotton fibers by using gossypium barbadense chromosome segment introgression line
CN102229980B (en) * 2011-05-17 2013-01-02 南京农业大学 Molecular breeding method for improving micronaire value of cotton by using chromosome segment introgression lines of island cotton
CN102229981B (en) * 2011-05-17 2013-04-10 南京农业大学 Molecular breeding method for improving strength and micronaire value of cotton fibers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
分子标记辅助选择的修饰回交聚合育种方法及其在棉花上的应用;郭旺珍等;《作物学报》;20051231;第31卷(第8期);第963-970页 *
陆地棉衣分及相关性状的遗传和QTL分子标记;张培通等;《江苏农业学报》;20051231;第21卷(第4期);第264-271页 *
陆地棉遗传图谱构建与纤维品质性状QTL定位;李朋波等;《西北植物学报》;20061231;第26卷(第6期);第1098-1104页 *

Also Published As

Publication number Publication date
CN103509861A (en) 2014-01-15

Similar Documents

Publication Publication Date Title
Salentijn et al. New developments in fiber hemp (Cannabis sativa L.) breeding
CN103571852B (en) Sea island cotton chromosome segment and a SSR marker thereof that significantly can increase upland cotton ginning outturn
CN103255139B (en) Major QTL (Quantitative Trait Locus) of cotton high-strength fiber and molecular marker and application thereof
CN102229979B (en) Molecular breeding method capable of improving length of cotton fibers by using gossypium barbadense chromosome segment introgression line
CN101492738B (en) Onion cytoplasmic male sterility SCAR mark and uses thereof
CN102805010B (en) Method for identifying chilli heat resistance by using detached leaves
CN105695478A (en) Gene for regulating plant types and yield of plants and application of gene
CN101760510A (en) Research and utilization progress of wheat dwarf stem gene
CN103866026A (en) Rice cold-resistant major gene identification method and special primer thereof
CN104170720A (en) Seed selection method for molecular marking assisted selection of high-resistance rice blast materials
CN103993011A (en) Molecular marker of sesame dominant genic male sterility gene and preparation method and application thereof
CN110305980B (en) Breeding method and application of anti-clubroot high-oleic-acid rape
CN105524999A (en) Molecular marker linked with sea island cotton fiber length of cotton
CN101487056B (en) Method for assistantly screening anti-stripe rust wheat, and special primer therefor
CN102229982B (en) Molecular breeding method for improving cotton fiber length, fiber strength, and micronaire value
CN107177667A (en) HRM molecular labelings chain wheat spike density QTL and its application
CN105483248A (en) Fiber strength related molecular mark from gossypium barbadense and application thereof
CN103866038B (en) For detecting tobacco to the N gene-specific primer of TMV resistance to, detection method and test kit
CN104109713B (en) With the multilocus assisted selection method that wheat conventional breeding whole process is combined
CN105506102A (en) Sea island cotton originated molecular markers related to intensity, length and micronaire value of fiber and application thereof
CN105200053A (en) Molecular markers derived from sea island cotton Hai 1 and associated with fiber length and applications of molecular markers
CN102229981B (en) Molecular breeding method for improving strength and micronaire value of cotton fibers
CN103509861B (en) Molecular breeding method for synchronously improving length, strength, and fineness of cotton fiber through polymerizing chromosome segment introgression lines
CN110468229A (en) Rice wide spectrum high resistance to hoja blanca gene Xa45's (t) isolates molecular labeling Hxjy-1
CN103834647B (en) Wheat Dwarfing gene Rht dC20closely linked SSR marker Xgwm537 and uses thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant