CN103255139B - Major QTL (Quantitative Trait Locus) of cotton high-strength fiber and molecular marker and application thereof - Google Patents
Major QTL (Quantitative Trait Locus) of cotton high-strength fiber and molecular marker and application thereof Download PDFInfo
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Abstract
The invention discloses a major QTL (Quantitative Trait Locus) of a cotton high-strength fiber and a molecular marker and application thereof. The major QTL of the cotton high-strength fiber is linked with two SSR (Simple Sequence Repeat) markers, is positioned through markers NAU/SSR/FS1195 and JESPR/SSR/FS2127 and is 0.01-2.39 centimeters away from a marker NAU3700; and the major QTL locus can explain 4.51%-17.55% of phenotype variation and achieves the LOD (Limit Of Detection) value between 3.23 and 7.09. The major QTL disclosed by the invention overcomes the defects of high cost, high difficulty and slow progress of quality breeding in the prior art, greatly increases the selection efficiency of the high-strength fiber by utilizing the major QTL locus of a cotton high-strength fiber gene and the molecular marker thereof, can be used for exploring high-quality fiber gene resources, provides the gene resources and a marker auxiliary selection technology for the quality breeding of cotton and can be applied to the production and quality detection of high-strength fiber cotton varieties.
Description
Technical field
The invention belongs to molecular genetic breeding field, relate to a high-strength cotton fibre main effect QTL and molecule marker thereof and application.
Technical background
Cotton fibre is important textile industry raw material, along with the continuous innovation of textile technology improves weaving efficiency to reduce costs energetically, air flow rotor spins, Air-Jet Spinning and FRICTION SPINNING technology eliminate old-fashioned RING SPINNING technology just rapidly, and these new textile technologies then have higher requirement to fibrous quality.Therefore in the urgent need to improving rapidly Cotton in China fibrous quality.The cotton variety output of Jin15Nian Lai China self-fertile is a little more than the U.S., but fibrous quality then some gap.The fibrous quality of China's govern-house-variety is medium, substantially can meet the requirement of textile industry, but quality is single, fibre strength is on the low side, and the high quality cotton of high-count yarn spun needs a large amount of import (Xiang Shikang etc., 1999, opinion Cotton in China Quality situation, Cotton Science, 11(1): 1-10).The cotton breeding worker of China is in order to improve cotton fiber interior quality, do large quantity research, but one side of making slow progress is the genetic resources in default of good fiber quality in enormous quantities, simultaneously also because fibrous quality and output exist larger negative correlation, the general more conventional commercial variety more than low one one-tenths of the strain output of cultivating, all fails popularizing planting on producing.
The research of american cotton quality breeding has the history of more than 50 year.The people such as Culp are the plan of PD Genetic improvement from nineteen forty-six, lasts for more than 40 years, has provided more than 30 germplasm line and 3 kinds.The fibrous quality of these germplasm lines or kind, especially fibre strength comparatively conventional variety are greatly improved, and output reaches the level of commercial variety.Since nineteen eighty-two, for adapting to the transformation of textile industry equipment, the U.S. accelerates the improvement to cotton fiber quality.Only 1982-1986 year encourages the bonus of Cotton Fiber Strength breeding just up to 5,600,000 dollars.From 1980, U.S.'s fibre strength improved 0.25g/tex every year, reached 21.7cN/tex to 1991 annuals.
Improve the fibrous quality level of existing Upland Cotton, first to collect high-intensity fiber germplasm line to do parent and existing high yield commercial variety and carry out a series of hybridization and hand over mutually, to break the negative correlation between intensity and output, but in often wheel backcrosses, all will detect fibrous quality, cost is very high, and to by the time receive the florescence terminate for some time after could knowledge of result, and require that breeding population is large, blindness is also large.Therefore, the cost of quality breeding is very high, and difficulty is comparatively large, makes slow progress.
Traditional Phenotypic Selection can be changed into direct Select gene type by molecular marker assisted selection, thus greatly improves efficiency of selection.In order to improve the efficiency of cotton fiber quality breeding, from 1994, the U.S. has carried out the molecule marking research of high-intensity fiber.Jiang etc. utilize sea-island and land hybrid F
2stock discrimination goes out 3 QTLss relevant with fibre strength, and Single locus can explain the phenotypic variation of 9.7% ~ 13.3%.Kohel etc. find 4 QTLs relevant with intensity equally in the hybrid generation of sea, land, and the effect of Single locus is between 10.4 ~ 23.1%; Utilize Upland Cotton Hybrids colony, Shapply etc. have found 6 QTLss relevant with intensity, and Ulloa etc. have found 3 QTLss relevant with intensity, can explain the variation of 10.6 ~ 24.1%.Up to the present, find that the effect of single QTL is between 9.7% ~ 24.0%.
Summary of the invention
The object of the invention is the above-mentioned deficiency for prior art, a high-strength cotton fibre main effect QTL is provided.
Another object of the present invention is to provide this main effect QTL molecule marker.
Another object of the present invention is to provide the application of this main effect QTL.
Object of the present invention realizes by following technical scheme:
High-strength cotton fibre gene main effect QTL site QTL qFS-D3-1 provided by the present invention, this main effect QTL has 2 SSR marker chain with it, by mark NAU/SSR/FS1
195, JESPR/SSR/FS2
127location, is respectively: NAU/SSR/Fs1, and the primer NAU3700 of sequence as shown in SEQ ID NO.1 and SEQ ID NO.2 can amplify the long DNA fragmentation of 195 pairs of Nucleotide; JESPR/SSR/Fs2, the primer JESPR101 of sequence as shown in SEQ ID NO.3 and SEQ ID NO.4 can amplify the long DNA fragmentation of 127 pairs of Nucleotide;
This main effect QTL site is positioned at cotton 17(D3) on karyomit(e), based on additive genetic effect, carry out the phenotypic variation being found this target main effect QTL soluble 4.51 ~ 17.55% in the recombinant inbred lines of hybridization acquisition by multiple years qualification at high-intensity fiber germplasm line Prema and upland cotton commercial variety 86-1.
Above-mentioned cotton fiber strength QTL site is obtain mark location by the following method: carry out hybridization by high-intensity fiber germplasm line Prema and 86-1 and form F
1by add year by year generation formed recombinant inbred lines be mapping population, the 8665 pairs of micro-satellite primers utilizing this research department to preserve carry out polymorphism screening to the parent of colony, the DNA of these polymorphism primers to each family of colony is utilized to carry out pcr amplification, thus determine the genotype of colony, utilize genetic mapping software JoinMap3.0 software building cotton chromosome collection of illustrative plates, meanwhile recombinant inbred lines is planted in Jiangpu 2009 respectively, Hainan 2009, Xinjiang 2010, multiple years plantation is carried out in Xinjiang 2011 and Jiangpu 2011, each two repetitions, carry out receipts to each family of colony spend and send to fine inspection, to obtain the fiber quality data of each family of colony, in conjunction with cotton chromosome collection of illustrative plates, utilize Windows QTLCartographer 2.5 software, adopt composite interval mapping method (Composite interval mapping), LR threshold value is that 11.5(is equivalent to LOD value 2.5), the QTL of 1000 test Analysis upland cotton fiber intensity.Result of study shows: have 16 fibre strengths QTL that is correlated with and be detected in five environment and Conjoint Analysis, explain the phenotypic variation of 3.73 ~ 17.55%, LOD value is between 2.61 and 7.09.These QTL are distributed on four karyomit(e)s of cotton, that is: 7(A7), 9(A9), 10(A10) and 17(D3) on karyomit(e); Except being positioned at 10(A10) on karyomit(e) the donor parents of two QTL be the additive effect that 86-1(shows as negative sense) except; The donor parents of all the other 15 QTL is the additive effect that Prema(shows as forward).Wherein: QTL qFS-A7-1 is detected in Sanya, Jiangpus in 2011 and Conjoint Analysis in 2009, explain the phenotypic variation of 4.1 ~ 7.44%, LOD value is between 3.3 and 3.82; QFS-A9-1 all can be detected in Aksu in 2010, Jiangpu, Shihezis in 2011 and Conjoint Analysis in 2011, and explain the phenotypic variation of 7.46 ~ 14.54%, LOD value is between 3.32 and 7.08; QFS-A10-2 is detected in Jiangpus in 2009 and Conjoint Analysis, and explain the phenotypic variation of 4.36 and 2.68% respectively, LOD value is respectively 2.61 and 2.58.
Be arranged in 17(D3) QTL qFS-D3-1 on karyomit(e) can in Jiangpu, Jiangsu in 2009, Sanya, Hainan, Aksu of Xinjiang in 2010, Jiangpu, Jiangsu in 2011, Shihezi of Xinjiang in 2011 and Conjoint Analysis in 2009 all can be detected, this QTL can explain the phenotypic variation of 4.51 ~ 17.55%, LOD value is between 3.23 and 7.09, and the cotton chromosome collection of illustrative plates QTL qFS-D3-1 delivered according to this laboratory is close to mark NAU/SSR/FS1
195and JESPR/SSR/FS2
127and range mark NAU/SSR/FS1
195at 0.01 ~ 2.39cM(Fig. 1).The correlation analysis of fibre strength under this mark and five environment is utilized to show, in 0.01 level, mark NAU/SSR/FS1
195with fibre strength all in extremely significantly positive correlation (table 1).
By the genome scanning of molecule marker, find at upland cotton Chr.17(D3) karyomit(e) there is a gene QTLfs that can significantly improve cotton fiber strength, there are 2 SSR marker, NAU/SSR/FS1
195, JESPR/SSR/FS2
127close linkage with it.
Utilize NAU/SSR/FS1
195pcr amplification is carried out in the cotton genomic dna of 192 kinds, the electrophoresis result of amplified production shows that having 25 kinds amplifies the band identical with parent Prema, wherein fibre strength has 10 kinds in the kind of more than 30cN/tex, account for 40% of sum, as described below: external kind comprises beautiful F-18, Su Yuan 7235, Soviet Union cotton 91 is, upland, Acala (large bell) B and AcalaSJ-1-9, and kind nearer with the sibship of Prema in it comprises Acala (large bell) B and AcalaSJ-1-9; Domestic kind mainly comprises bar state 5628, Ji A-7-8 (33 are), Handan long wool and middle money 9196(laser).
The molecule marking method of high-strength cotton fibre gene main effect QTL site QTL qFS-D3-1 of the present invention, extract cotton genomic dna, with the primer NAU3700 of sequence as shown in SEQ ID NO.1 and SEQ ID NO.2 or the primer JESPR101 of sequence as shown in SEQ ID NO.3 and SEQID NO.4, pcr amplification is carried out to the genomic dna extracted, if amplify with primer NAU3700 the DNA fragmentation that length is 195bp, or amplifying length with primer JESPR101 JESPR101 is the existence that the DNA fragmentation of 127bp then proves described high-strength cotton fibre gene main effect QTL site QTL qFS-D3-1.
The molecule marker of high-strength cotton fibre gene main effect QTL site QTL qFS-D3-1 of the present invention, molecule marker NAU/SSR/FS1
195primer NAU3700 forward primer is as shown in SEQ ID NO.1, and reverse primer is as shown in SEQ ID NO.2; Molecule marker NAU/SSR/FS1
195primer NAU3700 forward primer is as shown in SEQ ID NO.3, and reverse primer is as shown in SEQ ID NO.4.
The application of high-strength cotton fibre main effect QTL site QTL qFS-D3-1 of the present invention in the cotton variety of seed selection high-intensity fiber.
The application of molecule marker in the cotton variety of seed selection high-intensity fiber of high-strength cotton fibre main effect QTL site QTL qFS-D3-1 of the present invention.
Beneficial effect
Compared with prior art, tool has the following advantages and positively effect in the present invention:
1. above-mentioned high-strength cotton fibre gene main effect QTL site, obtain mark location by the following method: hybridized by high-intensity fiber germplasm line Prema and upland cotton commercial variety 86-1, carry out the test of multiple years by preparation RIL and binding molecule labeling technique is studied, the mode of inheritance finding Prema high microsteping intensity is additive genetic effect is main;
2. by the genome scanning of molecule marker, discovery is positioned at cotton 17(D3) on karyomit(e), there is a main effect QTL site QTLfs that can significantly improve cotton fiber strength, based on additive genetic effect, carry out at high-intensity fiber germplasm line Prema and upland cotton commercial variety 86-1 in the recombinant inbred lines of hybridization acquisition by multiple years qualification target main effect QTL site QTLfs, there are 2 closely linked marks, NAU/SSR/FS1
195, JESPR/SSR/FS2
127(Fig. 1), this main effect QTL site based on additive genetic effect, in the recombinant inbred lines of Prema × 86-1 soluble 4.51 ~ 17.55% phenotypic variation.
3. breeding practice proves that the main effect QTL site QTLfs of fibre strength is in different year, and varying environment performance is stable.This is that the fibrous quality level improving Cotton in China kind as early as possible lays the foundation.
4. to overcome the cost of quality breeding in prior art high in the present invention, difficulty is large, the defect of making slow progress, the high-strength cotton fibre gene main effect QTL site utilizing the present invention to develop and molecule marker thereof, greatly improve the efficiency of selection of high-intensity fiber, excavate out the fiber gene resource of high-quality, quality breeding for cotton provides the technology of genetic resources and marker assisted selection, thus greatly improve the efficiency of selection of high-intensity fiber, improve the fibrous quality of Cotton in China kind, can be applicable to production and the Quality Detection of high-intensity fiber cotton variety.
Accompanying drawing explanation
The QTL Molecular mapping result of the RIL fibre strength that Fig. 1 is detected by single environment and Conjoint Analysis
Note: above-mentioned environment respectively with " E " for representative, as described below: E1, Jiangpu in 2009; E2, Sanya in 2009; E3, Aksu in 2010; E4, Jiangpu in 2011; E5, Shihezi in 2011; The Conjoint Analysis of E6, E1 ~ E3.
Fig. 2. primer NAU3700 is at the amplification of the cotton variety of parent and different sources
Note: 1. protect 6716, 2. long wool 67-12, 3. Shanghai 749513, 4. brocade 444, 5. storehouse car T94-1, 6. Anhui 9 828, 7. Hubei Province 408, 8. Handan 8959, 9. Central China 91-0102, 10. blue or green anti-No. 1, 11. Sha 24-3, 12. No. 31, poplar graves, 13. precocious long wools 7, 14. river high ginning outturn 58, 15. Soviet Union Xu 138, ARR40682 in 16., 17.S-050019, 18. open Feng great Ling, 19. new large bells, the 20. large bells in Xinzhou, 21.Acala (large bell) B, ARR40681 in 22., 23. large peach cottons (giving), 24.GP70, cotton No. 11 (3389), 25. Shandongs, 26. No. 3, mountain agriculture, 27. nasal mucus 168, 28. Xuzhou 244, the anti-01-505 of 29. Zheng, in 30. 2108, Ji 926 in 31., 32.CZA (70) 33, 33. Shan 2747, 34. Shan 2800, 35. fortune 92 anti-124, in 36. 870203, BD27 is planted in 37., 640 are provided in 38., in 39. 521, cotton No. 5 of 40. shens, 41.AcalaSJ-1-9, R03 in 42., 43.UA887 (excellent), 44. CCRIs 35, 45.SGK stone selects 321, 46.Prema, 47.86-1
Embodiment
Embodiment 1
Implementation procedure of the present invention is: the upland cotton high-intensity fiber germplasm line Prema drawn from the U.S. is carried out Single-plant selection, and measure fibrous quality, select the stable strain Prema that isozygotys, according to the measurement result of 2000 and calendar year 2001, strain Prema 2 years fiber quality averages are respectively: fibre strength 35.63cN/tex, staple length 30.39mm, mic value 4.25, the anti-blight kind that Upland Cotton 86-1(is bred as by Chinese Academy of Agricultural Sciences Ma Cun researcher): fibre strength 25.70cN/tex, staple length 27.74mm, mic value 4.72.The open free granting of above two germplasm materials, generally utilizes in Cotton genetic breeding unit.If other colleagues need simultaneously, Agricultural University Of Nanjing's Cotton Research Institute can provide these kind of matter to studies in China unit.
Strain Prema and 86-1 is hybridized by year in summer in 2005, the end of the year F
1seed delivers to Hainan Island, and selfing produces F
2seed.Winter in 2006 at Sanya, Hainan by above-mentioned F
2:3another part kind in large Tanaka, and by individual plant selfing, produce F
4for seed, in each family, 1 selfing bell is collected in every strain, produces F after mixing spring in 2007 in the selfing of base, Jiangpu, Nanjing
5for seed, results often go to randomly draw 1 individual plant, gathers in the crops all selfing bells of this individual plant, produces F
6for seed, selfing also finally produces F in this way
8for seed, 2009 by F
8generation recombinant inbred lines and parent plant in base, Agricultural University Of Nanjing Jiangpu.The individual plant DNA of this segregating population is extracted by CTAB method.After blow-of-cottons, often row is chosen 5 middle individual plants and is received and spend, sample, carry out fibrous quality mensuration.All cotton samples all deliver to Earthquake of Anyang station in Henan Ministry of Agriculture fibrous quality and seed quality inspection center carries out fibrous quality inspection.
8665 pairs of SSR primers are utilized first to carry out initial analysis to the DNA polymorphism of Prema and 86-1 parent.SSR label primer all derives from website Cotton Marker Database(
http:// www.cottonmarker.org/) on the cotton micro-satellite primers sequence announced, synthesized by Shanghai Sheng Gong biotech company.The equal available from Sigma of the PCR such as Taq enzyme, dNTPs reaction reagent.PCR reaction volume is 10ul, wherein, and 67mM Tris-Hcl (PH8.8), 16mM (NH
4) SO
4, 2.5mM MgCl
2, 0.2mMdNTPs, 0.6uM primer, 0.5unit Taqase and genomic dna 20ng.PCR reaction is at Thermal Cycle 9600(Perkin-Elmer) upper amplification.PCR response procedures is: 94 DEG C of denaturations are after 5 minutes, 94 DEG C of sex change 45 seconds, 57 DEG C of annealing 45 seconds, and 72 DEG C extend 60 seconds, circulates 30 times, last 72 DEG C of extensions 7 points of kinds; Amplified production is electrophoresis in PAGE glue, then silver dye, and silver-colored dye process is: fixing (10% alcohol, 0.5% Glacial acetic acid) 12 minutes, 0.2%AgNO
3middle dyeing 12 minutes, washes 2 times, each 1 minute, colour developing in nitrite ion (1.5%NaOH, 1.0% formaldehyde), record result.Molecule marker results of preliminary screening shows, has 304 pairs of SSR primers variant on parents.These 304 pairs of Primer Analysis 180 RILs are separated individual plant, obtain 305 pleomorphism sites altogether.With the linkage map of JoinMap3.0 software building (Prema × 86-1) RIL colony, construct upland cotton genetic linkage map, collection of illustrative plates is containing 279 sites, and cover the genetic distance of 1576.24cM, the average genetic between mark is 5.83cM; Comprise 67 linkage groups, all corresponding on 26 karyomit(e)s of cotton, account for whole genomic 33.82%.Utilize Windows QTL Cartographer 2.5(Basten etc., 2001) software carries out fiber quality characteristics QTL detection, screen a reproducible QTL relevant with fibre strength, this QTL is positioned at 17(D3) karyomit(e), there are 2 SSR marker and its close linkage, are respectively NAU/SSR/FS1 and JESPR/SSR/FS2:
NAU/SSR/FS1, with NAU3700 primer (5'ATCACGGAAATCGGTAATGT3'(SEQ ID NO.1), reverse primer 5'CTCTTCCTTCACCTCTTCCA3'(SEQ ID NO.2)) DNA fragmentation that length is 195 pairs of Nucleotide can be amplified;
JESPR/SSR/FS2, with JESPR101 primer (5'CCAAGTCAAGGTGAGTTATATG3'(SEQ ID NO.3), reverse primer 5'GCTCTTTGTTACTGAAATGGG3'(SEQ ID NO.4)) DNA fragmentation that length is 127 pairs of Nucleotide can be amplified; These primers can directly synthesize in associated biomolecule technical service company and utilize.
In (Prema × 86-1) recombinant inbred lines, be arranged in 17(D3) QTL qFS-D3-1 on karyomit(e) can in Jiangpu, Jiangsu in 2009, Sanya, Hainan, Aksu of Xinjiang in 2010, Jiangpu, Jiangsu in 2011, Shihezi of Xinjiang in 2011 and Conjoint Analysis in 2009 all can be detected, this QTL can explain the phenotypic variation of 4.51 ~ 17.55%, LOD value is between 3.23 and 7.09, and the cotton chromosome collection of illustrative plates QTL qFS-D3-1 delivered according to this laboratory is close to mark NAU/SSR/FS1
195and JESPR/SSR/FS2, and range mark NAU/SSR/FS1
195at 0.01 ~ 2.39cM(Fig. 1).The correlation analysis of fibre strength under this mark and five environment is utilized to show, in 0.01 level, mark NAU/SSR/FS1
195with fibre strength all in extremely significantly positive correlation (table 1).
Table 1. marks NAU/SSR/FS1
195with the correlation analysis of fibre strength
Note: critical correlation coefficients is R
0.05=0.197R
0.01=0.257
C
1: NAU/SSR/FS1
195mark, C
2: be Jiangpu fibre strength in 2011, C
3be Shihezi fibre strength in 2011,
C
4be Sanya fibre strength in 2009, C
5be Jiangpu fibre strength in 2009, C
6it is Aksu fibre strength in 2010
Embodiment 2
Utilize NAU/SSR/FS1
195pcr amplification is carried out in the cotton genomic dna of 192 kinds, the electrophoresis result of amplified production shows that having 25 kinds amplifies the band identical with parent Prema, wherein fibre strength has 10 kinds in the kind of more than 30cN/tex, account for 40% of sum, as described below: external kind comprises beautiful F-18, Su Yuan 7235, Soviet Union cotton 91 is, upland, Acala (large bell) B and AcalaSJ-1-9, and kind nearer with the sibship of Prema in it comprises Acala (large bell) B and AcalaSJ-1-9; Domestic kind mainly comprises bar state 5628, Ji A-7-8 (33 are), Handan long wool and middle money 9196(laser).
Claims (1)
1. high-strength cotton fibre gene main effect QTL site
qFS-D3-1molecule marking method, it is characterized in that: extract cotton genomic dna, with the primer NAU3700 of sequence as shown in SEQ ID NO.1 and SEQ ID NO.2 or the primer JESPR101 of sequence as shown in SEQ ID NO.3 and SEQ ID NO.4, pcr amplification is carried out to the genomic dna extracted, if amplify with primer NAU3700 the DNA fragmentation that length is 195bp, or amplify with primer JESPR101 the existence that DNA fragmentation that length is 127 bp then proves high-strength cotton fibre gene main effect QTL site described in claim 1.
2
.high-strength cotton fibre gene main effect QTL site
qFS-D3-1molecule marker, it is characterized in that: with upland cotton high-intensity fiber germplasm line Prema genomic dna for template, use molecule marker NAU/SSR/FS1
195primer NAU3700 can amplify the DNA fragmentation that length is 195 pairs of Nucleotide, described molecule marker NAU/SSR/FS1
195primer NAU3700 forward primer is as shown in SEQ ID NO. 1, and reverse primer is as shown in SEQ ID NO.2; Use molecule marker NAU/SSR/FS1
195primer NAU3700 can amplify the DNA fragmentation that length is 127 pairs of Nucleotide, described molecule marker NAU/SSR/FS1
195primer NAU3700 forward primer is as shown in SEQ ID NO. 3, and reverse primer is as shown in SEQ ID NO.4.
3
.the application of high-strength cotton fibre main effect QTL site according to claim 1 in the cotton variety of seed selection high-intensity fiber.
4
.the application of high-strength cotton fibre main effect QTL site according to claim 1 in the molecular marker assisted selection of quick cotton fiber specific tenacity.
5
.the application of molecule marker in the cotton variety of seed selection high-intensity fiber in high-strength cotton fibre main effect QTL site according to claim 2.
6
.the application of molecule marker in the molecular marker assisted selection of quick cotton fiber specific tenacity in high-strength cotton fibre main effect QTL site according to claim 2.
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| CN103571852B (en) * | 2013-09-05 | 2016-01-13 | 南京农业大学 | Sea island cotton chromosome segment and a SSR marker thereof that significantly can increase upland cotton ginning outturn |
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| CN110055246B (en) * | 2019-05-09 | 2021-03-30 | 江苏省农业科学院 | Abnormal cotton chromosome segment capable of improving cotton fiber strength and molecular marker thereof |
| CN110093442B (en) * | 2019-05-09 | 2022-07-12 | 江苏省农业科学院 | SSR molecular markers related to cotton short stalk and high coat |
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| CN110551734B (en) * | 2019-09-16 | 2022-07-19 | 南京农业大学 | Upland cotton fiber strength gene GhUBX and application thereof |
| CN110616275B (en) * | 2019-10-25 | 2022-07-26 | 南京农业大学 | Molecular marker derived from Yttrium okamuni cotton and cotton fiber strength QTL (quantitative trait locus) linkage and application thereof |
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