CN104170720A - Seed selection method for molecular marking assisted selection of high-resistance rice blast materials - Google Patents
Seed selection method for molecular marking assisted selection of high-resistance rice blast materials Download PDFInfo
- Publication number
- CN104170720A CN104170720A CN201310724928.XA CN201310724928A CN104170720A CN 104170720 A CN104170720 A CN 104170720A CN 201310724928 A CN201310724928 A CN 201310724928A CN 104170720 A CN104170720 A CN 104170720A
- Authority
- CN
- China
- Prior art keywords
- rice
- rice blast
- selection
- disease
- resistant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a seed selection method for molecular marking assisted selection of high-resistance rice blast materials, and belongs to the field of rice breeding. Specific molecular marker is assisted to select the diseases resistance gene materials, and field diseases resistance identification is combined, so the seed selection target is clear and efficient. The method includes the following steps: 1, hybridizing a resistant parent containing rice blast resistant gene Pi40 with a susceptible parent Jijing 88 to construct an anti-infectious group, and identifying and screening the above progeny group; 2, extracting DNA of an individual anti-infectious strain, carrying out PCR amplification, and carrying out electrophoresis analysis; and 3, planting rice blast resistance gene Pi40-containng rice variety NG13 and susceptible variety Jijing 88 and the individual anti-infectious group progeny strain in a rice blast high incidence area Songyuan city in Jilin Province, inducing the rice blast under natural conditions, and respectively carrying out leaf and panicle blast resistance investigation in the seedling and mature stage. The field anti-infectious group is constructed, a molecular marker for screening the rice blast resistant gene Pi40 is used, and the disease resistance identification of a rice blast high incidence area is combined, so efficient directed improvement and cultivation of rice blast resistant new varieties are realized, and the method is of great practical significance for field breeding.
Description
Technical field
The present invention relates to a kind of selection of molecular marker assisted selection high resistant to rice blast material, especially adopt the rice material of specific molecular marker assisted selection containing rice blast resistance gene, in conjunction with field phenotypic character and Disease Resistance Identification, breeding goal is more clear and definite efficient, belongs to rice breeding field.
Background technology
Rice blast is that its distribution is very extensive by the microbial rice disease of rice blast.According to statistics, the whole world has more than 80 countries all to have generation, and the general popular time can be caused the underproduction of 10%-20%, reaches 40%-50% while seriously generation.Since the nineties in 20th century, there is area all at 3,800,000 hm in the year of China's rice blast
2above, lose every year several hundred million kilograms of paddy.Along with unification, centralization and the climatic influences of kind application, the harm of rice blast is also more and more serious.Although people have expended huge energy in anti-evil control and material drops into, be limited to the impact of all many factors, control efficiency is always unsatisfactory, especially in the serious time occurring of disease, has caused huge loss usually to production breeding.Take Jilin Province as example, the lucky round-grained rice 88 of the main kind that transplants rice seedlings in Jilin Province, occurs because Variety Disease-resistance sexual involution causes ear stem pest large area in recent years, has had a strong impact on the production of North Japonica Rice.The basic reason that produces this phenomenon is exactly that main breed is narrow to the anti-spectrum of the sick seedling of rice blast, and unification, and centralization plantation is general, once rice blast pathogen Epidemic Races changes like this, kind just declines rapidly to the resistance of rice blast.The rice varieties of therefore seed selection resistance of wide spectrum is very important.
At present, in Varieties Resistant To Rice Blast Breeding Process, breeding man mainly adopts pedigree method.Traditional pedigree method is by two parent's assembly, Cultivars preferentially from separate offspring, and disease-resistant phenotype depends on Natural infection or artificial infection qualification, differentiates that difficulty is large, and accuracy rate is very low.Owing to not understanding parent's disease-resistant gene type, breeder in parent's selection very blindly, normally magnanimity combo, magnanimity is selected offspring, workload is large, breeding efficiency is low.Utilize molecular labeling to select in conjunction with the disease-resistant performance in field, can greatly improve the accuracy and efficiency of resistant variety seed selection.
Resistance gene of rice blast Pi40 is derived from Australia wild rice (o.australiensits) main effect disease-resistant gene, to the stronger resistance of Pyricularia oryzae performance from northeast Jilin Province of China.Between the DNA marker S2539 and RM3330 of Pi40 (t) Primary Location on No. 6 the short arm of a chromosome of paddy rice, genetic distance is respectively 3.8cm and 2.4cm; By electronics (e-landing) technology of landing, on paddy rice Pseudomolecule3, in the contig of 1.8Mb (conting), identifying 14 BAC/PAC clones, is that the primer of 6 NBS-LRR structural designs is arranged in pac clone P0649C11.DNA marker P1, P2 and Pi40 close linkage.
Summary of the invention
For high efficiency selected high resistant to rice blast new varieties, the material that targeted, specific selection contains Pi40 gene offspring, the invention provides a kind of effectively selection.Utilize DNA marker P1 and P2 to carry out molecular marker assisted selection to blast resistance gene Pi40, select and Resistance Identification in conjunction with the proterties of the different segregative generations in field, to improve the efficiency of the disease-resistant new material of seed selection.
The invention provides following solution:
The lucky round-grained rice 88 of the rice material NG13 that utilization contains rice blast resistance gene Pi40 and susceptible material builds Kang Gan F2 colony.Utilize the individual plant that specific molecular marker P1, P2 screening is good containing field phenotypic character and contain Pi40, utilize the individual plant filtering out to backcross with the lucky round-grained rice 88 of Susceptible parent again, obtain backcross population BC
1f
1add generation in Hainan; The positive season sowing BC in Tianjin
1f
2seed, again utilize molecular labeling P1, P2 to screen rice blast resistance gene Pi40, simultaneously screen disease-resistant individual plant in conjunction with field phenotypic screen and Disease Resistance Identification, utilize disease-resistant individual plant to backcross with the lucky round-grained rice 88 of Susceptible parent again, obtain BC
2f
1colony adds generation in Hainan; The positive season sowing BC in Tianjin
2f
2seed, utilizes molecular labeling P1, P2 to screen rice blast gene Pi40, and the disease-resistant individual plant part filtering out is from accompanying each other generation, and another part continues to backcross with the lucky round-grained rice 88 of Susceptible parent; The backcross population BC that positive season of Tianjin obtains
3f
1add generation in Hainan, positive season of Tianjin is to BC
3f
2individual plant proceed disease-resistant gene detect, screening contain disease-resistant gene individual plant, BC
2f
3seed continue to add generation detect.At BC
2f
4generation and BC
3f
4for time individual plant sowing carry out the screening of disease-resistant gene, selected individual plant expands numerous planting experimentally, and picks out field phenotype similar to lucky round-grained rice 88, contains the good new lines of rice blast resistance gene Pi40 disease resistance.
Detect the primer pair whether rice blast resistance gene Pi40 exists, as P1 and P2.P1 primer sequence is 5 '-CAACAAACGGGTCGACAAAGG-3 ', and P2 primer sequence is 5 '-CCCCCAGGTCGTGATACCTTC-3 '.
Detect a method that whether has resistance gene of rice blast Pi40 in paddy DNA, taking sequence shown in P1 and P2 as primer, DNA to be detected increases.The band that amplified production contains NG13 is and contains resistance gene of rice blast Pi40, as shown in the 1st hole of Fig. 1.
A kind of selection of blast resisting material, taking the rice material that contains resistance gene of rice blast Pi40 as parent, hybridize with susceptible rice varieties, filial generation F2 segregation population individual plant is extracted to genomic DNA, carry out pcr amplification taking sequence shown in P1 and P2 as primer, in conjunction with field resistance and phenotypic evaluation, screening contains rice blast resistance gene Pi40 the good individual plant of disease resistance.
The DNA that above-mentioned primer pair preferably extracts rice seedling carries out Marker Identification, detects whether carry rice blast resistance gene Pi40, and this primer qualification result verifies in conjunction with the disease-resistant detection in field, and accuracy rate is higher.This primer pair can be used as the molecular labeling of Pi40 gene in rice anti-rice blast breeding.
Brief description of the drawings
Fig. 1 is molecular labeling P1, the P2 agarose gel electrophoresis figure at the resistance parent NG13 of rice blast resistance gene Pi40 and the amplified production of the lucky round-grained rice 88 of susceptible variety and offspring's segregation population:
M:2000bp molecular weight marker (fragment length is respectively 2000bp, 1000bp, 750bp, 500bp, 250,100bp)
1; NG132; Lucky round-grained rice 883,5,7,9,10,11 is susceptible individual plant banding pattern; 4,6,8,12 is disease-resistant individual plant banding pattern
Embodiment
Embodiment 1: containing the resistance parent of rice blast resistance gene Pi40 and the evaluation and screening of Susceptible parent lucky round-grained rice 88 hybridization structure Kang Gan colonies and progeny population
Resistance parent NG13 and the lucky round-grained rice 88 of Susceptible parent containing rice blast resistance gene Pi40 are hybridized, selfing again, then in selfing F2 generation, is carried out character screening and blast resisting ospc gene Pi40 screening, selects F2 individual plant 50 strains that plant height, breeding time, yielding ability etc. are similar to lucky round-grained rice 88 and contain disease-resistant gene Pi40; Then backcross for the first time with the lucky round-grained rice 88 of Susceptible parent, selfing obtains BC
1f
2, then carry out the screening of character screening and disease-resistant gene Pi40, choose the BC that proterties is good and contain disease-resistant gene Pi40
1f
2individual plant 50 strains; Then backcross for the second time with the lucky round-grained rice 88 of Susceptible parent, selfing obtains BC
2f
2, then carry out the screening of character screening and disease-resistant gene Pi40, choose the BC that proterties is good and contain disease-resistant gene
2f
2an individual plant part is from accompanying each other generation, and another part backcrosses for the third time with the lucky round-grained rice 88 of Susceptible parent again, and selfing obtains BC
3f
2, then carry out the screening of field phenotypic screen and disease-resistant gene Pi40, the good and BC that contains disease-resistant gene of selection traits
3f
2individual plant carries out selfing; BC
2f
2self progeny and BC
3f
2in self progeny, all carry out field phenotypic character screening and disease-resistant gene Pi40 qualification.To BC
2f
4and BC
3f
4, select to contain disease-resistant gene and the good strain of phenotype, local expand numerous, survey product, species test and rice blast resistance and totally identify, can select the new lines of blast resisting according to whole breeding flow process.
Embodiment 2: anti-sense individual plant DNA extraction, pcr amplification and electrophoretic analysis
One, DNA extracts (CTAB method)
Take 0.1～0.2g rice leaf and be cut into 1cm length, put in the centrifuge tube of extract (1M Tris PH8.0,100mM:5M Nacl, 1.0M EDTA, 20mM:10%CTAB, 2.0%) 800 μ l of 65 DEG C of preheatings.Put into and organize beveller, frequency 57.0HZ, rotating speed 1710N, vibrations 200S.65 DEG C of water-bath 30min, the light and handy shake of every 10min evenly.Add isopyknic phenol chloroform/isoamyl alcohol (25: 24: 1), gentle shake, makes into emulsion.Under room temperature (temperature is not less than 15 DEG C), the centrifugal 10min of 12000r/min, gets supernatant.Carefully extract supernatant 450 μ l, add the absolute ethyl alcohol (or isopyknic isoamyl alcohol) of 2 times of volumes of precooling, more than placing 20min under-20 DEG C of conditions, the centrifugal 5min of 5000r/min, collecting precipitation.70% ethanol washing precipitation 1～2 time.Open pipe lid, put clean place and dry, milky DNA precipitation is transparent.Finally add 100 μ l TE (Tris-Hcl110mM, PH8.0; EDTA1mM, PH8.0) dissolution precipitation, be diluted to 100 μ g/ml ,-20 DEG C save backup.
Two, pcr amplification:
1.PCR reaction system: 2 × Taq PCR Master Mix10 μ l, the each 0.5 μ l of primer of 10 μ M, the template DNA 1 μ l of 100ug/ml, ddH
2o8 μ l.
2.PCR response procedures: 95 DEG C of denaturation 3min; 95 DEG C of 30s, 65 DEG C of 30s, 72 DEG C of 1min, circulate 35 times; 72 DEG C are extended 10min, 4 DEG C of preservations.
Three, PCR detects
3% agarose gel electrophoresis testing result, application of sample 6 μ l PCR products, through goldview dyeing, application gel imaging system is taken pictures.
Embodiment 3: by containing the disease-resistant variety NG13 of rice blast resistance gene Pi40, the lucky round-grained rice 88 of susceptible variety and Kang Gan colony single-strain planting in Songyuan City, Jilin Province, rice blast district occurred frequently, bring out rice blast through natural conditions, seedling stage and maturing stage carry out respectively leaf pest and ear stem pest resistance investigation, unified standard is evaluated.
1. leaf pest field test
Leaf pest investigation Recording criteria:
0 grade anosis ... high resistance (HR)
1 grade of scab is the brown point of syringe needle shape size ... anti-(R)
2 grades of slightly large brown points ... anti-(R)
3 grades of round property are to oval-shaped grey scab, edge brown, 1～2 millimeter of scab diameter ... in anti-(MR)
4 grades of typical spindle scabs, long 1～2 centimetre, be conventionally confined between two veins, the area of causing harm is no more than 2% of leaf area ... middle sense (MS)
5 grades of typical spindle scabs, the area of causing harm is no more than 10% of leaf area ... middle sense (MS)
6 grades of typical spindle scabs, the area 10.1～25% of causing harm ... sense (S)
7 grades of typical spindle scabs, the area 25.1～50% of causing harm ... sense (S)
8 grades of typical spindle scabs, the area 50.1～75% of causing harm ... high sense (HS)
9 grades of whole blades are withered ... high sense (HS)
After natural induction in fields, the popular rice blast leaf pest in area, Songyuan City of Jilin Province are identified.Result shows, rice blast resistance gene Pi40 donor parents NG13 and No. 4, No. 6, No. 8, No. 12 disease-resistant individual plant performances disease-resistant (R), and the lucky round-grained rice 88 of susceptible variety is high to be felt when landlord plants, No. 3, No. 5, No. 7, No. 9, No. 10, No. 11 individual plant shows as susceptible (S).
2. panicle blast field test
Ear stem pest disease tassel yield Assessment for classification standard
0 grade anosis ... high resistance (HR)
1 grade of disease tassel yield is lower than 1～5% ... anti-(R)
3 grades of disease tassel yields are 5.1～10% ... in anti-(MR)
5 grades of disease tassel yields are 10.1～25% ... middle sense (MS)
7 grades of disease tassel yields are 25～50 ... sense (S)
9 grades of disease tassel yields are 50.1～100% ... high sense (HS)
After natural induction in fields, the popular rice blast panicle blast in area, Songyuan City of Jilin Province is identified.Result shows, rice blast resistance gene Pi40 donor parents NG13 and No. 4, No. 6, No. 8, No. 12 disease-resistant individual plant performances disease-resistant (R), and the lucky round-grained rice 88 of susceptible variety is high to be felt when landlord plants, No. 3, No. 5, No. 7, No. 9, No. 10, No. 11 individual plant shows as susceptible (S).
Seedling stage the method described in embodiment 2 of pressing extract genomic DNA, carry out pcr amplification taking P1 and P2 as primer, amplification is as shown in Figure 1: the banding pattern of rice blast resistance gene Pi40 donor parents NG13 and disease-resistant individual plant is containing 200bp and 120bp two bands, and the banding pattern of the lucky round-grained rice 88 of Susceptible parent and susceptible individual plant is containing 250bp and 200bp two bands.
Draw thus, utilize molecular labeling P1, P2 can identify preferably rice blast resistance gene Pi40, the material field performance resistance that contains Pi40 gene is better, and the material field performance that does not contain Pi40 gene is susceptible.
Claims (2)
1. the selection of molecular marker assisted selection high resistant to rice blast material, is characterized in that, comprises the following steps:
Taking the rice material NG13 that contains rice blast resistance gene Pi40 as maternal, the lucky round-grained rice 88 of conventional sense rice blast japonica rice variety is hybridized for male parent, offspring carries out orientation and selects, select offspring's individual plant of the lucky round-grained rice 88 of economical character deflection japonica rice variety, recycling molecular labeling detects, be combined in rice blast district occurred frequently simultaneously and carry out the disease-resistant qualification in field, select the new varieties of the lucky round-grained rice 88 with high resistant to rice blast.
2. selection as claimed in claim 1, it is characterized in that, orientation described in step (1) selects to utilize the rice material NG13 and the rear first selfing of lucky round-grained rice 88 hybridization of susceptible material that contain rice blast resistance gene Pi40, and selfing F2 generation carries out proterties selection and detects containing the individual plant of rice blast resistance gene Pi40.Selection traits F2 individual plant good and that contain disease-resistant gene once backcrosses with the lucky round-grained rice 88 of susceptible japonica rice variety again, and selfing obtains BC
1f
2, then carry out proterties selection and detect containing the individual plant of rice blast resistance gene Pi40.Good and the BC that contains disease-resistant gene Pi40 of selection traits
1f
2individual plant carries out secondary with the lucky round-grained rice 88 of susceptible japonica rice variety again and backcrosses, and selfing obtains BC
2f
2, then carry out proterties selection and detect containing the individual plant of rice blast resistance gene Pi40.Good and the BC that contains disease-resistant gene of selection traits
2f
2an individual plant part is from accompanying each other generation, and another part carries out three times with the lucky round-grained rice 88 of susceptible japonica rice variety again and backcrosses, and selfing obtains BC
3f
2, then carry out proterties selection and detect containing the individual plant of rice blast resistance gene Pi40.Good and the BC that contains disease-resistant gene of selection traits
3f
2individual plant carries out selfing.BC
2f
2self progeny and BC
3f
2in self progeny, all carry out field phenotypic character selection and disease-resistant gene Pi40 qualification, choose BC
2f
4and BC
3f
4contain the strain that disease-resistant gene phenotype is good, local expansion is numerous, survey product, species test and blast resistance identification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310724928.XA CN104170720A (en) | 2013-12-19 | 2013-12-19 | Seed selection method for molecular marking assisted selection of high-resistance rice blast materials |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310724928.XA CN104170720A (en) | 2013-12-19 | 2013-12-19 | Seed selection method for molecular marking assisted selection of high-resistance rice blast materials |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104170720A true CN104170720A (en) | 2014-12-03 |
Family
ID=51952295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310724928.XA Pending CN104170720A (en) | 2013-12-19 | 2013-12-19 | Seed selection method for molecular marking assisted selection of high-resistance rice blast materials |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104170720A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542249A (en) * | 2014-12-30 | 2015-04-29 | 天津天隆农业科技有限公司 | Novel method for quickly trans-breeding northern aromatic japonica rice variety |
| CN104542250A (en) * | 2014-12-30 | 2015-04-29 | 天津天隆农业科技有限公司 | Multi-gene high rice blast resistance material breeding method adopting marker assisted selection |
| CN105104147A (en) * | 2015-09-10 | 2015-12-02 | 广西大学 | Method for rapidly inducing rice blast for agar substrate seedling nursery |
| CN105104177A (en) * | 2015-09-21 | 2015-12-02 | 安徽省农业科学院水稻研究所 | Method for cultivating rice disease-resistant material by utilizing molecular marker |
| CN105400866A (en) * | 2015-09-07 | 2016-03-16 | 云南省农业科学院粮食作物研究所 | Broad spectrum rice-blast-resistant gene Pi40 SCAR marker and applications thereof |
| CN106489717A (en) * | 2016-09-28 | 2017-03-15 | 四川省农业科学院生物技术核技术研究所 | A kind of Rice Resistance To Rice Blast orderly improvement method |
| CN113265484A (en) * | 2021-06-11 | 2021-08-17 | 江苏里下河地区农业科学研究所 | Method for simultaneously selecting rice blast resistance genes Pi40 and Pi54 by using molecular markers |
| CN114410731A (en) * | 2021-12-02 | 2022-04-29 | 吉林省农业科学院 | Grate screen for screening rice blast resistant materials and application thereof |
-
2013
- 2013-12-19 CN CN201310724928.XA patent/CN104170720A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542249A (en) * | 2014-12-30 | 2015-04-29 | 天津天隆农业科技有限公司 | Novel method for quickly trans-breeding northern aromatic japonica rice variety |
| CN104542250A (en) * | 2014-12-30 | 2015-04-29 | 天津天隆农业科技有限公司 | Multi-gene high rice blast resistance material breeding method adopting marker assisted selection |
| CN105400866A (en) * | 2015-09-07 | 2016-03-16 | 云南省农业科学院粮食作物研究所 | Broad spectrum rice-blast-resistant gene Pi40 SCAR marker and applications thereof |
| CN105400866B (en) * | 2015-09-07 | 2021-03-23 | 云南省农业科学院粮食作物研究所 | SCAR marker of broad-spectrum rice blast disease-resistant gene Pi40 and application thereof |
| CN105104147A (en) * | 2015-09-10 | 2015-12-02 | 广西大学 | Method for rapidly inducing rice blast for agar substrate seedling nursery |
| CN105104177A (en) * | 2015-09-21 | 2015-12-02 | 安徽省农业科学院水稻研究所 | Method for cultivating rice disease-resistant material by utilizing molecular marker |
| CN106489717A (en) * | 2016-09-28 | 2017-03-15 | 四川省农业科学院生物技术核技术研究所 | A kind of Rice Resistance To Rice Blast orderly improvement method |
| CN113265484A (en) * | 2021-06-11 | 2021-08-17 | 江苏里下河地区农业科学研究所 | Method for simultaneously selecting rice blast resistance genes Pi40 and Pi54 by using molecular markers |
| CN114410731A (en) * | 2021-12-02 | 2022-04-29 | 吉林省农业科学院 | Grate screen for screening rice blast resistant materials and application thereof |
| CN114410731B (en) * | 2021-12-02 | 2024-02-27 | 吉林省农业科学院 | Grate screen for screening rice blast resistance material and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104170720A (en) | Seed selection method for molecular marking assisted selection of high-resistance rice blast materials | |
| US10939628B2 (en) | Resistance to ToLCNDV in melons | |
| CN101138313B (en) | Maize inbred line resistant to MRDV bred by using molecule making | |
| CN104542250A (en) | Multi-gene high rice blast resistance material breeding method adopting marker assisted selection | |
| Zhiyuan et al. | Molecular tagging of a major quantitative trait locus for broad‐spectrum resistance to Verticillium wilt in upland cotton cultivar prema | |
| Mehlenbacher | Advances in genetic improvement of hazelnut | |
| CN104313016B (en) | The molecular labeling of the QTL/ major gene resistance relevant with cotton verticillium wilt resistance | |
| CN105475120A (en) | Molecular breeding method for improving cold resistance of rice | |
| CN103993011A (en) | Molecular marker of sesame dominant genic male sterility gene and preparation method and application thereof | |
| EP3442324A1 (en) | Begomovirus-resistant melon plants | |
| CN110305980B (en) | Breeding method and application of anti-clubroot high-oleic-acid rape | |
| Jakešová et al. | Variation of morphological and agronomic traits in hybrids of Trifolium pratense× T. medium and a comparison with the parental species. | |
| CN101838645A (en) | A pair of cabbage turnip mosaic virus EST-SSR markers and application thereof | |
| CN110468229B (en) | Coseparation molecular marker Hxjy-1 of rice broad-spectrum high-resistance bacterial leaf blight gene Xa45(t) | |
| CN111374041A (en) | Method for selecting rice blast resistant rice material with molecular marker assistance | |
| CN104293906A (en) | SSR molecular mark for identification of self-incompatibility of brassica campestris ssp. chinensis Makino, and application thereof | |
| CN104560961B (en) | The dominant disease-resistant gene ZYMV 1 of cucurbita pepo ZYMV linkage molecule mark and its application | |
| CN101845504B (en) | Primer sequence for identifying resistance of cucumber against alternaria cucumerina and identification method thereof | |
| CN110999779B (en) | Method for synthesizing clubroot-resistant brassica napus by using polymerization breeding and biotechnology | |
| CN109862781A (en) | The gene transgression of two kinds of yield QTLs in cucumber plant | |
| EP4171203A1 (en) | Novel disease resistant melon plants | |
| CN112470918A (en) | Breeding method and application of transgenic insect-resistant high-coat-content cotton nuclear sterile dual-purpose line | |
| CN108770678B (en) | Cross breeding and breeding method of sea rice and japonica rice restorer | |
| CN105145333A (en) | Commercial population breeding method | |
| CN106916845A (en) | A kind of method that bluish dogbane transgenosis formulates cytoplasmic male sterile line |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141203 |