CN103493621B - A kind of method of accurate identification Bud Bursting Period in Rice cold resistance - Google Patents
A kind of method of accurate identification Bud Bursting Period in Rice cold resistance Download PDFInfo
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Abstract
A method for accurate identification and assessment Bud Bursting Period in Rice cold resistance, comprises the following steps: step one, break seed dormancy; Step 2, constant temperature vernalization; Step 3, low temperature stress; Step 4, normal temperature restoration ecosystem; Step 5, cold resistance evaluation; Step 5, cold-resistant Strength co-mputation.Cold resistance is evaluated according to the planting percent of identical growing way rice bud after low temperature stress, cold resistance is divided into: extremely strong, by force, in, weak, extremely weak five types, and by successional cold-resistant strength calculations, evaluation criterion is provided to cold resistance between matter not of the same race, effectively can avoid the error caused because of method of testing, and foundation can be provided for Bud Bursting Period in Rice cold resistance test not of the same race, thus rice breeding personnel choose reasonable cold resistance kind matter can be helped to carry out the improvement of rice varieties cold resistance, there is result objective, accuracy is high, and method is easy to operate, reproducible feature.
Description
Technical field
The present invention relates to a kind of rice cropping and breeding method, particularly a kind of method of accurate identification Bud Bursting Period in Rice cold resistance.
Background technology
Low temperature is a large limiting factor of paddy growth, is the significant problem affecting rice yield, and China is every year because the paddy of low temperature cold damage loss reaches 30 ~ 5,000,000,000 kg.All can there is chilling injury reaction in paddy rice whole period of growing.Wherein the chilling injury ratio of bud phase is easier to occur in live field and rice seedling bed, if the bud phase is not cold-resistant, will causes reduction and the seedling rot phenomenon of paddy rice planting percent, thus affect the foundation of Rice Photosynthesis colony, and cause the reduction of rice yield.Therefore, Bud Bursting Period in Rice cold resistance is one of very important important character of Rice Production.Bud Bursting Period in Rice cold resistance affects the key factor that rice bud grows, bud phase cold resistance is the ability that young shoot cell is supported the family, from the ability showing as young shoot in appearance and bring out green seedling, bud phase cold resistance and booting stage and flowering stage cold resistance have close relevant, be widely used in the Identification of Cold Tolerance of Rice Resources.
The Seedling Cold Tolerance of accurate identification and assessment rice germplasm can help rice breeding personnel choose reasonable cold resistance kind matter to carry out the improvement of rice varieties cold resistance.But the test method of current rice germplasm bud phase cold resistance and evaluation criteria disunity, there is larger error to result of the test in existing test method and assessment result,
In prior art, Bud Bursting Period in Rice cold resistance assay method is:
Every kind chooses full seed 100, first uses 45 DEG C ~ 50 DEG C high temperature treatment 48 hours, to break seed dormancy before test.Then seed is positioned in the culture dish of pad filter paper, vernalization in the insulating box of 30 DEG C after seed soaking.When bud grows to 5mm, take out and be placed in process 10d in 5 DEG C of illumination boxs.Then culture dish taken out and be placed in temperature higher than the indoor having sunlight of 20 DEG C, rice bud is restore normal growth.Place 7-10d " Invest, Then Investigate " and become number of live vaccine, and calculate seeding rate.Evaluate the power of bud phase cold resistance with planting percent, point 1 ~ 9 grade of evaluation, evaluation criterion refers to table 1, and three repetitions are established in test, using three mean values repeated as statistic unit.
Planting percent (%)=(number of live vaccine/sprout one-tenth total grain number) X100
Table 1 Bud Bursting Period in Rice cold resistance grade scale
Grading standard of cold tolerance at bud stage
The shortcoming of prior art is:
1, utilize 100 seeds to carry out vernalization, because kind bud ratio is different, cause last planting percent there are differences; In addition, because each kind germination vigor is different, the speed that germinates between the different seed of same kind is also variant, causes the seed of Pass Test condition the most finally (the long 5mm of bud) less, large according to the planting percent application condition that total grain number that sprouts calculates;
2, existing evaluation criteria grade scale is not quite similar, 1st grade and 3rd level in the grade scale that Han Longzhi etc. propose, therefore and the experimental error produced is larger 7th grade fast with the 9th grade of transition, and, but this evaluation criterion is but widely used in rice germplasm bud phase Identification of Cold Tolerance;
3, discontinuous evaluation criterion is utilized merely different rice germplasms can only to be carried out fuzzy classification as 1,3,5,7,9 etc., between than relatively large different rice germplasm when bud phase cold resistance difference, then easily make cold resistance there is larger difference rice germplasm and be still attributed to same cold-resistant type, and cold resistance difference less be attributed to different cold resistance types, acquired results error is larger.Therefore, when the cold resistance of more different rice germplasm, continuity value should be used to compare analysis and assessment.
Therefore one science accurate bud phase cold resistance appraisal procedure is more provided, effectively can avoids the error caused because of method of testing, and can provide according to just seeming particularly important for Bud Bursting Period in Rice cold resistance test not of the same race.
Summary of the invention
For solving above-mentioned prior art Problems existing, the present invention proposes a kind of method of accurate identification Bud Bursting Period in Rice cold resistance, effectively can avoid the error caused because of method of testing, and foundation can be provided for Bud Bursting Period in Rice cold resistance test not of the same race, thus rice breeding personnel choose reasonable cold resistance kind matter can be helped to carry out the improvement of rice varieties cold resistance, have result objective, accuracy is high, method is easy to operate, reproducible feature.
For achieving the above object, technical scheme of the present invention is:
A method for accurate identification Bud Bursting Period in Rice cold resistance, comprises the following steps:
Step one, break seed dormancy: every kind chooses enough substantial full seeds, first within 48 hours, breaks seed dormancy by 45 DEG C ~ 50 DEG C high temperature treatment before test;
Step 2, constant temperature vernalization: all seeds are put into clear water and soaked 1 day, then take out and put vernalization to the insulating box of 30 DEG C, when the bud of most of seed grows to 5mm, each 100 of the seed selecting the long 5mm of bud from every kind is positioned in the culture dish being lined with moistening filter paper;
Step 3, low temperature stress: all culture dishes placing seed are first placed in process 10d in 5 DEG C of illumination boxs;
Step 4, normal temperature restoration ecosystem: taken out by culture dish and be placed in temperature higher than the indoor having sunlight of 20 DEG C, rice bud is restore normal growth, place 7-10d " Invest, Then Investigate " and become number of live vaccine;
Step 5, cold resistance evaluation: according to formula: planting percent (%)=(becoming number of live vaccine/100) × 100 calculate seeding rate; Evaluate the power of bud phase cold resistance with planting percent, according to planting percent by each kind independently cold resistance be divided into 1,3,5,7,9 five grade to evaluate, determine each grading standard and name;
Step 6, cold-resistant Strength co-mputation: use the calculated value of the resistance to cold strength of continuity to compare cold resistance between kind.
Bud Bursting Period in Rice cold resistance evaluation index and classification called after in described step 5:
1 grade: planting percent >90%, leaf look dark green, and cold resistance is extremely strong;
3 grades: 90% >=planting percent >70%, cold resistance is strong;
5 grades: 70% >=planting percent >50%, in cold resistance;
7 grades: 50% >=planting percent >10%, cold resistance is weak;
9 grades: 10% >=planting percent, cold resistance is extremely weak.
In described step 6, the computational methods of resistance to cold strength are:
1 grade: 1-(planting percent (%)-90)/10, the 0≤<1 of resistance to cold strength;
3 grades: 3-(planting percent (%)-70)/10, the 1≤<3 of resistance to cold strength;
5 grades: 5-(planting percent (%)-50)/10, the 3≤<5 of resistance to cold strength;
7 grades: 7-(planting percent (%)-10)/20, the 5≤<7 of resistance to cold strength;
9 grades: 9-planting percent (%)/5,7≤resistance to cold strength≤9.
Relative to prior art, beneficial effect of the present invention is:
1, utilize 100 to sprout and the long seed being 5mm of bud carries out low temperature stress process, avoid because kind bud ratio is different, cause last planting percent there are differences; It also avoid because each kind germination vigor is different, the speed that germinates between the different seed of same kind is variant and cause the quantity seed of final Pass Test condition less, solves and calculates seeding rate the large problem of error according to total grain number that sprouts;
2, can judge the accurate cold resistance of science of all carrying out between same kind and different cultivars, solve existing evaluation criteria grade scale and be not quite similar thus cause the skimble-scamble problem of the assessment result scope of application;
3, when the cold resistance of more different rice germplasm, use successional cold-resistant intensity level to compare analysis and assessment to solve and utilize merely discontinuous evaluation criterion different rice germplasms can only be carried out fuzzy classification as 1,3,5,7,9 etc., between than relatively large different rice germplasm when bud phase cold resistance difference, then easily make cold resistance there is larger difference rice germplasm and be still attributed to same cold-resistant type, and cold resistance difference less be attributed to different cold resistance types, the problem that acquired results error is larger.
Embodiment
The present invention is set forth further below by concrete test example:
Devise two tests utilizing existing test method and the inventive method to carry out the cold resistance assessment of bud phase and qualification, compare the quality of two kinds of methods in qualification and Evaluated effect:
1, existing method is utilized to carry out rice varieties bud phase Identification of Cold Tolerance and assessment:
Experimental scheme:
Test and bud phase Identification of Cold Tolerance has been carried out to 23 paddy rice Xian kind morning (combination), experimental scheme is carried out with reference to forefathers' test method, be specially: every kind chooses full seed 100, first uses 45 DEG C ~ 50 DEG C high temperature treatment 48 hours, to break seed dormancy before test.Then seed is positioned in the culture dish of pad filter paper, vernalization in the insulating box of 30 DEG C after seed soaking.When bud grows to 5mm, take out and be placed in process 10d in 5 DEG C of illumination boxs.Then culture dish taken out and be placed in temperature higher than the indoor having sunlight of 20 DEG C, rice bud is restore normal growth.Place 10d " Invest, Then Investigate " and become number of live vaccine, and calculate seeding rate.The evaluation criterion of bud phase cold resistance is with reference to the evaluation method should depositing [2] such as mountain [1] and Han Longzhi: the power evaluating bud phase cold resistance with planting percent, point 1 ~ 9 grade of evaluation, and evaluation criterion refers to table 2, using three mean values repeated as statistic unit.Between each kind, bud phase cold resistance significant difference SAS software is analyzed.
Planting percent (%)=(number of live vaccine/sprout one-tenth total grain number) X100
Table 2 Bud Bursting Period in Rice cold resistance grade scale
Tab.1Grading standard of cold tolerance at bud stage
2, results and analysis
The bud phase cold resistance of tested variety the results are shown in Table 3, shows bud phase cold resistance between different kinds and there are differences.
The cold resistance performance of table 3 different cultivars bud phase
* be the cold resistance rank after refinement, obtain according to differential calculating with planting percent ratio.
The bud phase cold resistance significance of different cultivars compares in table 4, and between display kind, bud phase cold resistance exists significant difference.
Table 4 different cultivars bud phase cold resistance significance compares
* be the cold resistance rank after refinement, obtain according to differential calculating with planting percent ratio.
3, have problems
3,1, table 2 shows, 100 seeds choosing Dormancy breaking in advance in test carry out the cold resistance test of bud phase, cause different cultivars on the bud ratio of final Pass Test condition (5mm bud is long), there is very large difference (number that sprouts from 2-80 not etc.).The difference of bud ratio will impact final percentage of green plantlets, and the accuracy of impact test.
(planting percent (%)=(number of live vaccine/sprout one-tenth total grain number) X100);
And, because the grain weight of some kind Pass Test condition (5mm bud is long) is very little, three as morning 17 good in test are repeated to only have 12 respectively, 7 and 13 test samples, good educate 948 three and repeat also only have 20 respectively, 18 and 18 test samples, as everyone knows, small sample amount will cause bigger error, the accuracy of impact test.
If test chooses enough seed Dormancy breaking and vernalization in advance, the long seed of 5mm bud therefrom choosing eligible requirement is tested, the bud ratio of each kind is all 100, then can avoid the experimental error caused because of the difference of bud ratio, also improve sample size, the final accurate rate improving test.(planting percent (%)=(becoming number of live vaccine/100) X100);
2, in test, the evaluation criterion reference of bud phase cold resistance should deposit the evaluation method of [2] such as mountain [1] and Han Longzhi, in this standard 99% and following planting percent to be all attributed to 3rd level other, too fast to rank 3 transition from rank 1, evaluation result can not objective reaction kind real bud phase cold resistance.As in this test, a lot of kind difference repeat planting percent all more than 90%, some or even more than 96%, be but attributed to rank 3, belong to strong cold resistance rank.The problem that the same same scope existed between the 7th with the 9th rank is too little or excessive.This kind of grade scale can not carry out objective evaluation to the resistance of kind.Liu et al。, (2004)
[3], propose more rational bud phase cold resistance evaluation criterion (table 5), this standard all brings out into green seedling more than 90% young shoot, leaf look viridescent be attributed to the 1st rank; Planting percent is classified as the 7th grade between 10%-50%, and planting percent is classified as the 9th grade below 10%, and this grade scale is more reasonable, can carry out classified estimation objectively to the resistance of kind.
Table 5 Bud Bursting Period in Rice cold resistance grade scale
Grading standard of cold tolerance at bud stage
3, in result of the test, the planting percent of kind is continuous type numerical value, and the bud phase cold resistance evaluation criteria of kind is discontinuity numerical value, utilize discontinuity numerical value to be described kind can only to be carried out fuzzy classification to continuity numerical value, the Difference of Cold Tolerance between different cultivars in same group can not be objectively responded.Between the kind utilizing discontinuity numerical value to carry out, Difference of Cold Tolerance significance compares and all 23 kinds is divided into A, B, C, D tetra-class, most of kind concentrates on one of them type, as excellent in I in table 3 899, grand flat 006, T excellent 898, Rong You 463, 03 excellent 66, gold excellent 418, Jinyou 458, the excellent L2 of gold, strain two excellent 505, the cold resistance rank of He You 27 grades morning is all 3, but how concrete interracial difference can not display, and interracial significant difference also can not carry out trickle comparison because of utilizing continuity to carry out processing, when in the qualification a large amount of rice varieties (combination) or variety resources of rice being carried out to cold resistance, assessment and when comparing, this situation is especially outstanding, accuracy and the objectivity of whole test are had a strong impact on.If continuity numerical value can be utilized to carry out interracial otherness compare, then greatly can improve the accuracy of analysis of experiments result.
For the issuable error of above-mentioned test, utilize the inventive method again these 23 kinds to be carried out to qualification and the assessment of bud phase cold resistance, to compare the superiority of new method of the present invention, be specially:
2, the inventive method is utilized to carry out the testing and appraisal of bud phase cold resistance to same rice varieties
Experimental scheme:
A method for accurate identification Bud Bursting Period in Rice cold resistance, comprises the following steps:
Step one, break seed dormancy: every kind chooses enough substantial full seeds, first within 48 hours, breaks seed dormancy by 45 DEG C ~ 50 DEG C high temperature treatment before test;
Step 2, constant temperature vernalization: all seeds are put into clear water and soaked 1 day, then take out and put vernalization to the insulating box of 30 DEG C, when the bud of most of seed grows to 5mm, each 100 of the seed selecting the long 5mm of bud from every kind is positioned in the culture dish being lined with moistening filter paper;
Step 3, low temperature stress: all culture dishes placing seed are first placed in process 10d in 5 DEG C of illumination boxs;
Step 4, normal temperature restoration ecosystem: taken out by culture dish and be placed in temperature higher than the indoor having sunlight of 20 DEG C, rice bud is restore normal growth, place 7-10d " Invest, Then Investigate " and become number of live vaccine;
Step 5, cold resistance evaluation: according to formula: planting percent (%)=(becoming number of live vaccine/100) × 100 calculate seeding rate; Evaluate the power of bud phase cold resistance with planting percent, according to planting percent by each kind independently cold resistance be divided into 1,3,5,7,9 five grade to evaluate, determine each grading standard and name;
Step 6, cold-resistant Strength co-mputation: use the calculated value of the resistance to cold strength of continuity to compare cold resistance between kind.
Bud Bursting Period in Rice cold resistance evaluation index and classification called after in described step 5:
1 grade: planting percent >90%, leaf look dark green, and cold resistance is extremely strong;
3 grades: 90% >=planting percent >70%, cold resistance is strong;
5 grades: 70% >=planting percent >50%, in cold resistance;
7 grades: 50% >=planting percent >10%, cold resistance is weak;
9 grades: 10% >=planting percent, cold resistance is extremely weak.
In described step 6, the computational methods of resistance to cold strength are:
1 grade: 1-(planting percent (%)-90)/10, the 0≤<1 of resistance to cold strength;
3 grades: 3-(planting percent (%)-70)/10, the 1≤<3 of resistance to cold strength;
5 grades: 5-(planting percent (%)-50)/10, the 3≤<5 of resistance to cold strength;
7 grades: 7-(planting percent (%)-10)/20, the 5≤<7 of resistance to cold strength;
9 grades: 9-planting percent (%)/5,7≤resistance to cold strength≤9.
Between each kind, bud phase cold resistance significant difference is analyzed according to the successive value SAS software of the resistance to cold strength of each kind.
1, test results and analysis
Utilize the bud phase cold resistance performance of the different cultivars of the inventive method gained in table 6.The interracial cold resistance level difference of distinct methods compares in table 7, utilizes continuity difference to carry out cold resistance significant difference comparative result in table 8 to kind.
The cold resistance performance of table 6 different cultivars bud phase
Table 7, different tests method cold resistance compare
* be the cold resistance rank after refinement, obtain according to differential calculating with planting percent ratio
Relatively prior art and the inventive method are in qualification and the accuracy assessing rice varieties bud phase Difference of Cold Tolerance, can find:
1, the seed of the long uniformity of bud of same quantity is employed in the present invention's test due to same kind, the sample size of each kind Pass Test condition is consistent, do not occur as in prior art test as in good morning 17, only have 12 respectively in three repetitions, the bud of 7 and 13 Pass Test conditions enters test.The error that can effectively reduce because test material causes tested by large sample.
2, test method of the present invention is utilized can to make error-reduction between the repetition of same kind difference.Utilize test method of the present invention, the planting percent numerical value Relatively centralized that same kind difference repeats, the cold resistance of large multi items is all in same rank, and minority kind is in two adjacent ranks, and (as prosperous in the Gan in art methods 203 3 are repeated to be respectively 3 not appear at the phenomenon of crossing over three ranks in the test method of prior art, 5,7 three ranks, three of five excellent 157 are repeated, and are 1,3,7 three ranks), improve the accuracy of test.
3, classification identification standard of the present invention is utilized, by planting percent higher than 90% be attributed to extremely strong cold resistance, can avoid from 1 grade excessive too fast to 3 grades, and the span of rank 3 is excessive, make planting percent be greater than more than 70 be all attributed to same resistance to cold strength, more reasonably can locate the cold resistance rank of kind, reduce the staging error because experimental error causes.(three repetition values of L2 planting percent as excellent in gold are respectively 98.39,98.61 with 72.41, statistically the 3rd repetition values and the first two repetition values have significant difference, but grade scale conventionally, their cold resistance intensity still belongs to same rank, and error is obviously very large).
4, adopt continuity numerical value to carry out cold resistance strength ratio between kind and comparatively, utilize new standard, the nuance (table 8) of cold resistance between different cultivars can be shown well.
Table 8 utilizes the kind cold resistance significance of continuity difference to compare
* compare for utilizing continuity difference to carry out kind cold resistance significant difference
Table 8 is visible, if only utilize discontinuous cold resistance intensity level to compare, kind is only divided into A, B, C, D tetra-ranks, most of kind is all in the rank of ABCD, and utilize continuous type numerical value to carry out cold resistance between kind to compare, the cold resistance of kind is then divided into 8 ranks from A to H, Category disposition between each rank, the Difference of Cold Tolerance of different cultivars can be distinguished better, especially, when testing kind quantity and being many (such as identifying and assess the bud phase cold resistance of variety resources of rice), the superiority of continuity value is used more can to embody.
2, two kinds of appraisal and evaluation Measures compare
As can be seen from comparative test result, Bud Bursting Period in Rice cold resistance test method of the present invention, cold resistance level evaluation standard and authentication method can reduce experimental error well, improve the accurate rate of test, reasonably the bud phase cold resistance of different rice varieties is identified, assesses and compared, relative to test and the evaluation method of prior art, there is the advantage of the following aspects in the inventive method:
1, test method of the present invention can test raising sample size effectively, reduces the experimental error because the difference of test material causes, thus improves the accurate rate of test.
2, grade scale of the present invention can avoid because of grading range narrow (from 1 grade to 3 grades) or wide (from 7 grades to 9 grades) cause planting percent exist significant difference be but still in same cold resistance rank phenomenon occur, reasonably strength grading is carried out to the cold resistance of kind.
3, utilize continuity numerical value to the cold resistance intensity between different cultivars, can the nuance of more different rice varieties bud phase cold resistance effectively, for the selection of variety resources of rice and utilization provide more detailed and reliable data.
3, sum up
Bud Bursting Period in Rice cold resistance affects the key factor that rice bud grows, and the Seedling Cold Tolerance of accurate identification and assessment rice germplasm can help rice breeding personnel choose reasonable cold resistance kind matter to carry out the improvement of rice varieties cold resistance.The test method of current rice germplasm bud phase cold resistance and evaluation criteria disunity, larger error is there is in existing test method and assessment result to result of the test, existing test method, qualification and evaluation criteria are improved and revised, effectively can avoid the error caused because of method of testing, improve the accuracy of test and assessment, greatly improve the availability of Rice Germplasm Resources and the success rate of cold resistance breeding.
This test indicates test method in the past, qualification and the drawback existing for evaluation criteria, and constructively test method is improved, carry out correcting and refinement to qualification and evaluation criteria, utilize test method of the present invention can avoid the error caused because test material is different, utilize new qualification and evaluation criteria can carry out objective analysis and trickle qualification to the cold resistance of different cultivars, the appraisal and evaluation for Rice Germplasm Resources bud phase cold resistance is from now on provided objective, method and a standard accurately by this test method and appraisal and evaluation standard.
Claims (2)
1. a method for accurate identification Bud Bursting Period in Rice cold resistance, is characterized in that, comprises the following steps:
Step one, break seed dormancy: every kind chooses enough substantial full seeds, first within 48 hours, breaks seed dormancy by 45 DEG C ~ 50 DEG C high temperature treatment before test;
Step 2, constant temperature vernalization: all seeds are put into clear water and soaked 1 day, then take out and put vernalization to the insulating box of 30 DEG C, when the bud of most of seed grows to 5mm, each 100 of the seed selecting the long 5mm of bud from every kind is positioned in the culture dish being lined with moistening filter paper;
Step 3, low temperature stress: all culture dishes placing seed are first placed in process 10d in 5 DEG C of illumination boxs;
Step 4, normal temperature restoration ecosystem: taken out by culture dish and be placed in temperature higher than the indoor having sunlight of 20 DEG C, rice bud is restore normal growth, place 7-10d " Invest, Then Investigate " and become number of live vaccine;
Step 5, cold resistance evaluation: according to formula: planting percent (%)=(becoming number of live vaccine/100) × 100 calculate seeding rate; Evaluate the power of bud phase cold resistance with planting percent, according to planting percent by each kind independently cold resistance be divided into 1,3,5,7,9 five grade to evaluate, determine each grading standard and name;
Step 6, cold-resistant Strength co-mputation: use the calculated value of the resistance to cold strength of continuity to compare cold resistance between kind, the computational methods of resistance to cold strength are:
1 grade: 1-(planting percent (%)-90)/10, the 0≤< of resistance to cold strength 1;
3 grades: 3-(planting percent (%)-70)/10, the 1≤< of resistance to cold strength 3;
5 grades: 5-(planting percent (%)-50)/10, the 3≤< of resistance to cold strength 5;
7 grades: 7-(planting percent (%)-10)/20, the 5≤< of resistance to cold strength 7;
9 grades: 9-planting percent (%)/5,7≤resistance to cold strength≤9.
2. the method for a kind of accurate identification Bud Bursting Period in Rice cold resistance as claimed in claim 1, is characterized in that, Bud Bursting Period in Rice cold resistance evaluation index and classification called after in described step 5:
1 grade: planting percent > 90%, leaf look dark green, and cold resistance is extremely strong;
3 grades: 90% >=planting percent > 70%, cold resistance is strong;
5 grades: 70% >=planting percent > 50%, in cold resistance;
7 grades: 50% >=planting percent > 10%, cold resistance is weak;
9 grades: 10% >=planting percent, cold resistance is extremely weak.
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| CN105432391B (en) * | 2015-12-29 | 2018-05-18 | 江西省农业科学院水稻研究所 | A kind of method for promoting ridged pot without plow bud ratio and planting percent |
| CN105875225B (en) * | 2016-04-18 | 2018-12-11 | 山西省农业科学院小麦研究所 | A method of the wheat frost of resistance to spring is identified using blade freeze injury |
| CN107432222A (en) * | 2016-12-29 | 2017-12-05 | 中国科学院植物研究所 | A kind of authentication method of the cold-resistant phenotype of rice seedling |
| CN107576683A (en) * | 2017-09-11 | 2018-01-12 | 福建省热带作物科学研究所 | A kind of rapid identification method of Moringa cold resistance |
| CN111386790A (en) * | 2020-04-24 | 2020-07-10 | 黑龙江省农业科学院耕作栽培研究所 | Method for identifying low-temperature germination capacity of direct-seeded rice in cold region |
| CN114190132A (en) * | 2020-09-17 | 2022-03-18 | 沈阳师范大学 | Method for evaluating cold resistance of rice in bud stage |
| CN112634072A (en) * | 2020-12-29 | 2021-04-09 | 沈阳师范大学 | Evaluation method for cold resistance of rice in seedling stage based on principal component analysis |
| CN113016267B (en) * | 2021-03-01 | 2022-05-03 | 浙江大学 | Rice pre-harvest sprouting evaluation method based on pre-harvest sprouting seed grading standard |
| CN113287474A (en) * | 2021-05-20 | 2021-08-24 | 黑龙江省农业科学院水稻研究所 | Screening method for cold-resistant rice |
| CN114568066B (en) * | 2022-03-22 | 2023-04-25 | 中国农业科学院棉花研究所 | Method for screening cotton emergent seedling stage cold-resistant varieties and application thereof |
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