CN102265746A - Method for testing tobacco drought resistances in seedling stage - Google Patents
Method for testing tobacco drought resistances in seedling stage Download PDFInfo
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- CN102265746A CN102265746A CN2011101064543A CN201110106454A CN102265746A CN 102265746 A CN102265746 A CN 102265746A CN 2011101064543 A CN2011101064543 A CN 2011101064543A CN 201110106454 A CN201110106454 A CN 201110106454A CN 102265746 A CN102265746 A CN 102265746A
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Abstract
The invention discloses a method for testing tobacco drought resistances in a seedling stage. The method is carried out in a greenhouse and comprises the following steps of: (1) selecting varieties; (2) raising seedlings; (3) drying; (4) recovering to supply water; (5) drying again; (6) recovering to supply water again; and (7) testing and judging the drought resistances of the tobacco varieties. According to the method for testing the tobacco drought resistances in the seedling stage disclosed by the invention, the drought resistances of the tobacco varieties are rapidly tested in the seedling stage by processing the drought on a seedling raising float tray; a technical system for testing the tobacco drought resistances in the seedling stage is established for the first time; drought resistance characteristics of different tobacco varieties can be clearly distinguished through a two-stage drought method; and the drought resistance evaluations of the tobaccos have relative standards by adopting screened moderate-resistance contrast varieties and high-resistance contrast varieties. By using the technical system, the drought resistance evaluations to a great number of tobacco varieties and germplasm resources can be realized.
Description
Technical field
The present invention relates to the authentication method of plant drought resistance, specifically, relate to the method for identifying the tobacco drought resistance.
Background technology
The drought resisting authenticate technology in other crop as, wheat, corn etc. research more.Corns etc. also have the report of identifying in seedling stage.Because the crop difference, authentication method is widely different.The report major part of tobacco drought resisting research is to adopt potted plant arid to handle, and the cycle is longer, time-consuming taking a lot of work, and technical system is immature.Tobacco drought resisting authenticate technology is immature always, and the main cause that is difficult to use is, never finds suitable drought resisting check variety in the tobacco, does not have contrast just to be difficult to estimate the drought resistance of identifying general kind; Do not see yet and be fit to degree of drought's related data that the tobacco drought resisting is identified.
The technical scheme that relates to crop drought resistance in the Chinese patent database has No. 200410061011.7 " utilizing rice drought induced gene promoter LEAP improvement plant drought resistance ", No. 200710178721.1 " a kind of drought resistance appraising method ", No. 200810036342.3 " utilizing mouse nitrous oxide synthetase gene to improve the drought resistance of plant ", No. 200910272694.3 " application of MAPKKK family gene DSM1 gene in the control paddy drought resistance " etc., but up to now, do not see the process patent that the tobacco drought resistance is identified.
Summary of the invention
The object of the present invention is to provide the method for identifying the tobacco drought resistance seedling stage,, solve a difficult problem of identifying the research of tobacco bred drought resistance to realize drought resistance evaluation to a large amount of tobacco breds and germ plasm resource.
Identify the method for tobacco drought resistance seedling stage provided by the invention, carries out in the greenhouse, and specific practice is: (1) kind is selected: tobacco bred and check variety that need to select evaluation; (2) grow seedlings: in the greenhouse, adopt the floating seedlings method to grow seedlings each kind 50～70 strain, 3 repetitions; (3) arid is handled: when about 60 days seedling ages of cigarette seedling, all floating plates of growing seedlings are taken out from nursery pond, be put on the shelf, cut off all water sources, environmental conditions such as the residing illumination of all kinds, ventilation are consistent, the control temperature is 30 ℃ in the greenhouse, is not less than 20 ℃ (being first order arid processing/mild drought) night; (4) resume water supply: observe cigarette seedling arid situation, anti-check variety is wilted in the time of can not recovering naturally in treating, all kinds are put into nursery pond again, resumes water supply; (5) arid is handled once more: after arid processing (mild drought) resumed water supply 5 days first, the damage caused by a drought index carried out once more arid less than 40 kind and handles (being the arid processing/Severe drought in the second level), and processing method is arid first together to be handled; (6) resume water supply once more: when treating that high anti-check variety can not recover naturally (3-5 level), again the cigarette seedling is put into water and recovered 5 days; (7) identify: before arid is handled the back rehydration and the rehydration level record of keeping the score after 5 days, handle, resume water supply for twice by twice arid, record tobacco recovery capability is differentiated the drought resistance of tobacco bred.
Anti-check variety in described in the said method, the promptly stable tobacco bred that shows as the moderate drought resistance, kind commonly used is the Majiang Caulis et folium clerodendri bungei; The anti-check variety of described height, the promptly stable tobacco bred that shows as the height drought resistance, kind commonly used is No. 26, Oxford.
The inventor points out: generally the check variety in researchs such as disease-resistant, drought resisting there is no definite regulation, and various researchs all are that employing is customary, and everybody generally acknowledges the higher check variety of degree.But tobacco drought resisting identification research lacks check variety always, anti-contrast (Majiang Caulis et folium clerodendri bungei) and high anti-to contrast (No. 26, Oxford) two contrasts be that the inventor passes through the screening study of hundreds of tobacco breds and definite in determining among the present invention, fairly obvious through its drought resisting feature of practice test, can solve the tobacco drought resisting and identify the problem that lacks check variety.
The division of above-mentioned drought resistance:
Being divided into of record cigarette strain arid reflection grade:
Damage caused by a drought formula of index: RI=(∑ (XiNi)/4N) * 100
In the formula: RI is the aridity index, and Xi is corresponding arid grade strain number,
Ni is each arid grade, and N is the total strain number of investigation.
Identify the method for tobacco drought resistance seedling stage of the present invention, adopt the arid on the floating plate of growing seedlings to handle the drought resistance that realizes Rapid identification tobacco bred in seedling stage, set up the tobacco in seedling stage drought resisting authenticate technology system of system first; Can clearly distinguish the drought resisting feature of different tobacco breds by two-stage arid method, adopt filter out in anti-check variety and high anti-check variety, make the evaluation of tobacco drought resistance that the relative standard arranged.This technical system can realize the drought resistance evaluation to a large amount of tobacco breds and germ plasm resource.
Embodiment
Embodiment is to the drought resistance identification research of 12 tobacco breds
(1) get 12 tobacco breds and add 2 check varieties, adopt floating seedlings, seedling management carries out routinely, and 3 repetitions are established in each kind 70 strain (half floating plate); Preparing arid during about 60 days seedling ages handles; (2) all are grown seedlings floating plate takes out from nursery pond, is put on the shelf and (cuts off all water sources), and environmental conditions such as the residing illumination of all kinds, ventilation are consistent.Control temperature 30 degree in the greenhouse are not less than 20 degree (being first order arid processing/mild drought) night.(3) observe cigarette seedling arid situation, anti-contrast (Majiang Caulis et folium clerodendri bungei) is wilted and can not be recovered naturally in the 4th day finds.All kinds are put into nursery pond again, resume water supply.When (4) the cigarette seedling recovered 5 days in water, most of kind was restored (arid rank is 0 grade), but 2,3,9, No. 11 four strains can not restore.Each kind arid situation is put down in writing in classification for the first time, and will all kinds of four strains are taken into from nursery pond once more except that 2,3,9, No. 11, is put into to carry out arid processing on the shelf processing method the same (being the arid processing/Severe drought in the second level).(5) observe cigarette seedling arid situation, find that at the 8th day height resists contrast (No. 26, Oxford) to wilt and can not recover naturally.To handle the cigarette seedling same day puts into nursery pond once more and resumes water supply.When (6) the cigarette seedling recovered 5 days in water, high anti-contrast (No. 26, Oxford) was extensive substantially former, and other kind all has arid symptom in various degree.The record of classification for the second time.
Qualification result such as following table:
Subordinate list: kind drought resisting qualification result in seedling stage
Claims (3)
1. identify the method for tobacco drought resistance seedling stage, carry out in the greenhouse that its feature comprises: (1) kind is selected: tobacco bred and the check variety of selecting needs to identify; (2) grow seedlings: in the greenhouse, adopt the floating seedlings method to grow seedlings each kind 50～70 strain, 3 repetitions; (3) arid is handled: when about 60 days seedling ages of cigarette seedling, all floating plates of growing seedlings are taken out from nursery pond, be put on the shelf, cut off all water sources, environmental conditions such as the residing illumination of all kinds, ventilation are consistent, and the control temperature is 30 ℃ in the greenhouse, is not less than 20 ℃ night; (4) resume water supply: observe cigarette seedling arid situation, anti-check variety is wilted in the time of can not recovering naturally in treating, all kinds are put into nursery pond again, resumes water supply; (5) arid is handled once more: after arid processing first resumed water supply 5 days, the damage caused by a drought index carries out once more arid less than 40 kind to be handled, and processing method is with arid processing first; (6) resume water supply once more: when treating that high anti-check variety can not recover naturally, again the cigarette seedling is put into water and recovered 5 days; (7) identify: before arid is handled the back rehydration and the rehydration level record of keeping the score after 5 days, handle, resume water supply for twice, differentiate the drought resistance of tobacco bred by twice arid.
2. method according to claim 1, it is characterized in that described in anti-check variety, be the stable tobacco bred that shows as the moderate drought resistance; The anti-check variety of described height is the stable tobacco bred that shows as the height drought resistance.
3. method according to claim 1 is characterized in that described drought resistance is divided into:
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102598982A (en) * | 2012-03-20 | 2012-07-25 | 中国烟草中南农业试验站 | Method for testing cold resistance of tobacco in seedling stage |
| CN102860199A (en) * | 2012-09-03 | 2013-01-09 | 安徽省农业科学院水稻研究所 | Rapid screening method for drought-enduring variety of rice at seedling stage |
| CN103109687A (en) * | 2013-03-14 | 2013-05-22 | 安徽省农业科学院烟草研究所 | Rapid high-flux sieving method of tobacco drought-resistant idioplasm |
| CN103843561A (en) * | 2014-02-24 | 2014-06-11 | 贵州省烟草科学研究院 | Drought-resistance seed sieving method |
| CN104542268A (en) * | 2015-01-14 | 2015-04-29 | 青岛农业大学 | High-throughput screening method of composite resistance seeds of tobaccos |
| CN105340597A (en) * | 2015-10-10 | 2016-02-24 | 甘肃省农业科学院作物研究所 | Crop seedling stage drought resistance identification method employing bottom upward percolation re-watering technology |
| CN105917990A (en) * | 2016-04-17 | 2016-09-07 | 山西省农业科学院经济作物研究所 | Identification method for drought resistance of soybean |
| CN106879333A (en) * | 2017-02-23 | 2017-06-23 | 河北省农林科学院旱作农业研究所 | A kind of millet flower-pot cover imbibition once arid drought resistance of seedling authentication method |
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| CN101221159A (en) * | 2007-12-04 | 2008-07-16 | 上海市农业生物基因中心 | Drought resistance appraising method |
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| CN101221159A (en) * | 2007-12-04 | 2008-07-16 | 上海市农业生物基因中心 | Drought resistance appraising method |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102598982A (en) * | 2012-03-20 | 2012-07-25 | 中国烟草中南农业试验站 | Method for testing cold resistance of tobacco in seedling stage |
| CN102598982B (en) * | 2012-03-20 | 2013-04-03 | 中国烟草中南农业试验站 | Method for testing cold resistance of tobacco in seedling stage |
| CN102860199A (en) * | 2012-09-03 | 2013-01-09 | 安徽省农业科学院水稻研究所 | Rapid screening method for drought-enduring variety of rice at seedling stage |
| CN102860199B (en) * | 2012-09-03 | 2015-07-29 | 安徽省农业科学院水稻研究所 | A kind of rice seedling drought-enduring variety rapid screening method |
| CN103109687A (en) * | 2013-03-14 | 2013-05-22 | 安徽省农业科学院烟草研究所 | Rapid high-flux sieving method of tobacco drought-resistant idioplasm |
| CN103843561A (en) * | 2014-02-24 | 2014-06-11 | 贵州省烟草科学研究院 | Drought-resistance seed sieving method |
| CN103843561B (en) * | 2014-02-24 | 2016-01-13 | 贵州省烟草科学研究院 | A kind of screening technique of drought resistance kind matter |
| CN104542268A (en) * | 2015-01-14 | 2015-04-29 | 青岛农业大学 | High-throughput screening method of composite resistance seeds of tobaccos |
| CN105340597A (en) * | 2015-10-10 | 2016-02-24 | 甘肃省农业科学院作物研究所 | Crop seedling stage drought resistance identification method employing bottom upward percolation re-watering technology |
| CN105340597B (en) * | 2015-10-10 | 2018-07-24 | 甘肃省农业科学院作物研究所 | Using the identification method for the crops seedling stage drought resistance for oozing rehydration on bottom |
| CN105917990A (en) * | 2016-04-17 | 2016-09-07 | 山西省农业科学院经济作物研究所 | Identification method for drought resistance of soybean |
| CN106879333A (en) * | 2017-02-23 | 2017-06-23 | 河北省农林科学院旱作农业研究所 | A kind of millet flower-pot cover imbibition once arid drought resistance of seedling authentication method |
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