CN103483055A - Apple tree feed and preparation method thereof - Google Patents
Apple tree feed and preparation method thereof Download PDFInfo
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- CN103483055A CN103483055A CN201310380741.2A CN201310380741A CN103483055A CN 103483055 A CN103483055 A CN 103483055A CN 201310380741 A CN201310380741 A CN 201310380741A CN 103483055 A CN103483055 A CN 103483055A
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Abstract
The invention discloses an apple tree feed and a preparation method thereof. The preparation method is characterized in that beneficial microorganisms are subjected to pure culture and mixed fermentation and then are mixed with polyacrylamide, epsilon-polylysine, matrine, calcium propionate, ascorbic acid, EDTA (Ethylene Diamine Tetraacetic Acid), potassium fulvic and ZnCl2 so as to obtain the apple tree feed. The apple tree feed has stable structure and wide functions without toxic or side effects, can be used for replacing a chemical fertilizer in agricultural production, serves as a spraying type fertilizer in planting-type agriculture, particularly has an obvious effect on apple trees, and can form a layer of effective protective layer on the surfaces of apples so as to prevent the apples from oxidizing, inhibit the growth of harmful microorganisms and effectively prevent the apples from browning, so that the quality of the products is improved, and the yield of the products is increased.
Description
Technical field
The present invention relates to the microbial fertilizer technical field, particularly relate to a kind of apple tree fertilizer that prevention apple brown stain effect is arranged and preparation method thereof.
Background technology
Diversified microorganism organic fertilizer is arranged now on the market, and the today of day by day pursuing more high-quality life people, the fertilizer of exploitation highly effective and safe has become the trend of development.And existing fruit tree fertilizer much all has strong toxic side effect to human body mostly for can cause drug residue, and cost is high, complex manufacturing.Due to the excessive use of chemical fertilizer and the plantation year after year of crop, soil starts big area and hardens, and the profitable strain in soil is fewer and feweri, and the soil-borne diseases such as continuous cropping, root knot nematode are day by day serious; Also can cause that the tree body is thin and weak, stripping, disease resistance are not poor, various physiological disturbances and infectious disease take place frequently, and spray-type fertilizer can be avoided these problems.In addition, apple is favorite in people's daily life and universal face one of the widest a kind of fruit, variable color, softening and rotten phenomenon very easily appear in apple, especially the apple brown stain is modal problem, low temperature, controlled atmosphere and Chemical Preservative are the most effectual ways of current apple fresh-keeping, but the ubiquity cost high, the problems such as drug residue are arranged.A kind of from the fruit tree fertilizer of apple development phase with regard to the problem of taking into account prevention apple brown stain, be the problem that the present invention need to solve.
Summary of the invention
Purpose of the present invention is exactly that a kind of spray-type apple tree fertilizer that has the brown stain of prevention apple concurrently and preparation method thereof is provided for the defect of above-mentioned existence.By beneficial microorganism after pure culture and complex ferment again with polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2be mixed to get.This fertilizer structure is stable, function is extensive, have no side effect, can in agriculture production, replace chemical fertilizer in the Implant agricultural as spray-type fertilizer, especially for the apple tree successful, at apple surface, can form the effective protective layer of one deck, prevent the apple oxidation, suppress the harmful microorganism growth, effectively prevent the apple brown stain, improve quality and the productive rate of product.
A kind of apple tree fertilizer of the present invention and preparation method thereof technical scheme is that this fertilizer contains polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2and complex micro organism fungicide.
Described complex micro organism fungicide is yeast, lichengermium, subtilis, photosynthetic bacteria, streptomyces microflavus, bacillus laterosporus.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 10-15 hundred million/ml, lichengermium 10-25 hundred million/ml, subtilis 10-25 hundred million/ml, photosynthetic bacteria 100-150 hundred million/ml, streptomyces microflavus 10-15 hundred million/ml, bacillus laterosporus 10-25 hundred million/ml.
This fertilizer specifically contains following component by weight: polyacrylamide 5-25 part, epsilon-polylysine 5-10 part, matrine 3-5 part, calcium propionate 3-5 part, xitix 3-5 part, EDTA3-5 part, potassium fulvate 5-10 part, ZnCl
23-10 part and microbiobacterial agent 50-150 part, all the other are water.
The preparation method of described a kind of apple tree fertilizer, step is:
(1) obtain respectively the fermented liquid of each bacterial classification after the bacterial classification in complex micro organism fungicide is fermented separately respectively, then the fermented liquid of single culture is mixed to ferment obtaining complex micro organism fungicide;
(2) by polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mix, stir;
(3) complex micro organism fungicide obtained in step (1) is fully mixed to thin up with the mixture in step (2).
In step (1), the bacterial classification in complex micro organism fungicide carries out the single culture fermentation and adopts one or more in beef-protein medium, potato culture, LB substratum.
After in step (1), the bacterial classification in complex micro organism fungicide being fermented separately respectively, inoculum size by the fermented liquid of single culture by 1-3% all is inoculated in the mixed strains substratum, wherein, the mixed strains substratum contains polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture aqueous solution, fermented, obtain complex micro organism fungicide.
In step (1), in the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture and the mass ratio of water be 2-5:100.
In the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture aqueous solution in add the zinc sulfate that massfraction is 0.3%-1%.
In step (3), the complex micro organism fungicide obtained in step (1) is fully mixed with the mixture in step (2), and weight ratio is polyacrylamide 5-25 part, epsilon-polylysine 5-10 part, matrine 3-5 part, calcium propionate 3-5 part, xitix 3-5 part, EDTA3-5 part, potassium fulvate 5-10 part, ZnCl
23-10 part and microbiobacterial agent 50-150 part, after thin up, the zinc sulfate that the interpolation massfraction is 0.3%-1%.
Beneficial effect of the present invention is: fertilizer of the present invention and preparation method be by beneficial microorganism after pure culture and complex ferment again with polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2be mixed to get, broken through the antagonistic action of existing complex microorganism product, the zinc sulfate that adds in addition 0.3%-1% after fermenting-ripening liquid mixes has so not only been eliminated the antagonistic action of many bacterial classifications after compound, and has increased synergy each other.
This fertilizer structure is stable, function is extensive, have no side effect, at aspects such as plant husbandry and environmental protection, good application prospect arranged, can in agriculture production, replace chemical fertilizer in the Implant agricultural as spray-type fertilizer, especially for the apple tree successful, can form the effective protective layer of one deck at apple surface, prevent the apple oxidation, suppress the harmful microorganism growth, effectively prevention apple brown stain, quality and the productive rate of raising product.
Fertilizer of the prior art adopts the finished product microbial inoculum to mix with filler mostly, lacks fermentating metabolism product, drying course excess Temperature in addition, and the microorganism loss is serious, has had a strong impact on the fertilizer efficiency of fertilizer product.These problems that preparation method of the present invention fundamentally solves, make the fertilizer efficiency of product of the present invention have greatly and improve.
embodiment:
In order to understand better the present invention, below with specific examples, describe technical scheme of the present invention in detail, but the present invention is not limited thereto.The original strain that the present invention uses is all enough to be obtained in Chinese agriculture microbial strains preservation administrative center (Agricultural Culture Collection of China english abbreviation ACCC), and the raw material that the present invention uses all adopts chemical pure.
Embodiment 1
A kind of apple tree fertilizer, 10 parts of polyacrylamides, 5 parts of epsilon-polylysines, 5 parts of matrines, 3 parts of calcium propionates, 3 parts, xitix, EDTA3 part, 5 parts of potassium fulvates, ZnCl
2100 parts of 5 parts and microbiobacterial agents, all the other are water.
Preparation process is:
(1) obtain respectively the fermented liquid of each bacterial classification after yeast, lichengermium, subtilis, photosynthetic bacteria, streptomyces microflavus, bacillus laterosporus are fermented separately respectively, the single culture fermentation adopts one or more in beef-protein medium, potato culture, LB substratum.
The fermented liquid of single culture all is inoculated in the mixed strains substratum by 1% inoculum size, wherein, to contain weight part be 10 parts of polyacrylamides, 5 parts of epsilon-polylysines, 5 parts of matrines, 3 parts of calcium propionates, 3 parts, xitix, EDTA3 part, 5 parts of potassium fulvates, ZnCl to the mixed strains substratum
2the mixture aqueous solution of 5 parts, fermented, and obtains complex micro organism fungicide.In the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture and the mass ratio of water be 3:100.In the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture aqueous solution in add the zinc sulfate that massfraction is 0.5%.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 1,500,000,000/ml, lichengermium 2,000,000,000/ml, subtilis 2,000,000,000/ml, photosynthetic bacteria 15,000,000,000/ml, streptomyces microflavus 1,000,000,000/ml, bacillus laterosporus 2,000,000,000/ml.
(2) 10 parts of polyacrylamides, 5 parts of epsilon-polylysines, 5 parts of matrines, 3 parts of calcium propionates, 3 parts, xitix, EDTA3 part, 5 parts of potassium fulvates, ZnCl
25 parts of mixing, stir;
(3) complex micro organism fungicide obtained in step (1) is fully mixed with the mixture in step (2), after 100 times of thin ups, the zinc sulfate that to add massfraction be 0.3%-1%, obtain apple tree fertilizer A.
Embodiment 2
A kind of apple tree fertilizer, 5 parts of polyacrylamides, 25 parts of epsilon-polylysines, 3 parts of matrines, 3 parts of calcium propionates, 5 parts, xitix, EDTA5 part, 10 parts of potassium fulvates, ZnCl
2150 parts of 3 parts and microbiobacterial agents, all the other are water.
Preparation process is:
(1) obtain respectively the fermented liquid of each bacterial classification after yeast, lichengermium, subtilis, photosynthetic bacteria, streptomyces microflavus, bacillus laterosporus are fermented separately respectively, the single culture fermentation adopts one or more in beef-protein medium, potato culture, LB substratum.
The fermented liquid of single culture all is inoculated in the mixed strains substratum by 3% inoculum size, wherein, to contain weight part be 5 parts of polyacrylamides, 25 parts of epsilon-polylysines, 3 parts of matrines, 3 parts of calcium propionates, 5 parts, xitix, EDTA5 part, 10 parts of potassium fulvates, ZnCl to the mixed strains substratum
2the mixture aqueous solution of 3 parts, fermented, and obtains complex micro organism fungicide.In the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture and the mass ratio of water be 2:100.In the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture aqueous solution in add the zinc sulfate that massfraction is 1%.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 1,000,000,000/ml, lichengermium 2,500,000,000/ml, subtilis 1,000,000,000/ml, photosynthetic bacteria 10,000,000,000/ml, streptomyces microflavus 1,500,000,000/ml, bacillus laterosporus 1,000,000,000/ml.
(2) 5 parts of polyacrylamides, 25 parts of epsilon-polylysines, 3 parts of matrines, 3 parts of calcium propionates, 5 parts, xitix, EDTA5 part, 10 parts of potassium fulvates, ZnCl
23 parts of mixing, stir;
(3) complex micro organism fungicide obtained in step (1) is fully mixed with the mixture in step (2), after 200 times of thin ups, the zinc sulfate that to add massfraction be 1%, obtain apple tree fertilizer B.
Embodiment 3
A kind of apple tree fertilizer, 5 parts of polyacrylamides, 10 parts of epsilon-polylysines, 3 parts of matrines, 5 parts of calcium propionates, 5 parts, xitix, EDTA5 part, 8 parts of potassium fulvates, ZnCl
250 parts of 10 parts and microbiobacterial agents, all the other are water.
Preparation process is:
(1) obtain respectively the fermented liquid of each bacterial classification after yeast, lichengermium, subtilis, photosynthetic bacteria, streptomyces microflavus, bacillus laterosporus are fermented separately respectively, the single culture fermentation adopts one or more in beef-protein medium, potato culture, LB substratum.
The fermented liquid of single culture all is inoculated in the mixed strains substratum by 2% inoculum size, wherein, to contain weight part be 5 parts of polyacrylamides, 10 parts of epsilon-polylysines, 3 parts of matrines, 5 parts of calcium propionates, 5 parts, xitix, EDTA5 part, 8 parts of potassium fulvates, ZnCl to the mixed strains substratum
2the mixture aqueous solution of 10 parts, fermented, and obtains complex micro organism fungicide.In the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture and the mass ratio of water be 5:100.In the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture aqueous solution in add the zinc sulfate that massfraction is 0.3%.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 1,200,000,000/ml, lichengermium 1,500,000,000/ml, subtilis 1,500,000,000/ml, photosynthetic bacteria 13,000,000,000/ml, streptomyces microflavus 1,200,000,000/ml, bacillus laterosporus 1,200,000,000/ml.
(2) 5 parts of polyacrylamides, 10 parts of epsilon-polylysines, 3 parts of matrines, 5 parts of calcium propionates, 5 parts, xitix, EDTA5 part, 8 parts of potassium fulvates, ZnCl
210 parts of mixing, stir;
(3) complex micro organism fungicide obtained in step (1) is fully mixed with the mixture in step (2), after 100 times of thin ups, the zinc sulfate that to add massfraction be 0.8%, obtain apple tree fertilizer C.
Embodiment 4
A kind of apple tree fertilizer, 5 parts of polyacrylamides, 10 parts of epsilon-polylysines, 5 parts of matrines, 4 parts of calcium propionates, 4 parts, xitix, EDTA4 part, 8 parts of potassium fulvates, ZnCl
2120 parts of 10 parts and microbiobacterial agents, all the other are water.
Preparation process is:
(1) obtain respectively the fermented liquid of each bacterial classification after yeast, lichengermium, subtilis, photosynthetic bacteria, streptomyces microflavus, bacillus laterosporus are fermented separately respectively, the single culture fermentation adopts one or more in beef-protein medium, potato culture, LB substratum.
The fermented liquid of single culture all is inoculated in the mixed strains substratum by 1% inoculum size, wherein, to contain weight part be 5 parts of polyacrylamides, 10 parts of epsilon-polylysines, 5 parts of matrines, 4 parts of calcium propionates, 4 parts, xitix, EDTA4 part, 8 parts of potassium fulvates, ZnCl to the mixed strains substratum
2the mixture aqueous solution of 10 parts, fermented, and obtains complex micro organism fungicide.In the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture and the mass ratio of water be 3:100.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 1,500,000,000/ml, lichengermium 1,000,000,000/ml, subtilis 2,200,000,000/ml, photosynthetic bacteria 11,000,000,000/ml, streptomyces microflavus 1,500,000,000/ml, bacillus laterosporus 1,000,000,000/ml.
(2) 5 parts of polyacrylamides, 10 parts of epsilon-polylysines, 5 parts of matrines, 4 parts of calcium propionates, 4 parts, xitix, EDTA4 part, 8 parts of potassium fulvates, ZnCl
210 parts of mixing, stir;
(3) complex micro organism fungicide obtained in step (1) is fully mixed with the mixture in step (2), 300 times of thin ups, obtain apple tree fertilizer D.
Embodiment 5
For the effect of the fertilizer that better explanation the present invention makes, product of the present invention has been carried out to field test, process of the test is as follows:
One, test design,
1. spray fertilizer of the present invention
By fertilizer A, B in embodiment 1-4, C, D foliage-spray 2 times.
2. spray fertilizer+conventional fertilizer application of the present invention
By fertilizer A, B in embodiment 1-4, C, D foliage-spray 2 times, coordinate conventional soil application.
3. conventional fertilizer application
Carry out conventional soil application.
Two, trial crops
Trial crops is the fruiting period apple tree.
Three, experimental result
After ripening stage arrives, tri-kinds of experiment statisticses of 1.2.3 the results are shown in Table 1.
Table 1
As can be seen from the above table, output, browning rate and the insect pest rate data of experiment 1 and experiment 2 are more or less the same, but increase than simple conventional fertilizer application output, browning rate and insect pest rate obviously reduce, as can be seen here, apple tree fertilizer of the present invention can in agriculture production, replace chemical fertilizer in the Implant agricultural as spray-type fertilizer, especially for the apple tree successful, can form the effective protective layer of one deck at apple surface, prevent the apple oxidation, suppress the harmful microorganism growth, effectively prevent the apple brown stain, improve quality and the productive rate of product.
Claims (10)
1. an apple tree fertilizer, is characterized in that, contains polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2and complex micro organism fungicide.
2. a kind of apple tree fertilizer according to claim 1, is characterized in that, described complex micro organism fungicide is yeast, lichengermium, subtilis, photosynthetic bacteria, streptomyces microflavus, bacillus laterosporus.
3. a kind of apple tree fertilizer according to claim 2, it is characterized in that, in described complex micro organism fungicide, the content of each bacterial classification is: yeast 10-15 hundred million/ml, lichengermium 10-25 hundred million/ml, subtilis 10-25 hundred million/ml, photosynthetic bacteria 100-150 hundred million/ml, streptomyces microflavus 10-15 hundred million/ml, bacillus laterosporus 10-25 hundred million/ml.
4. a kind of apple tree fertilizer according to claim 3, it is characterized in that, contain following component by weight: polyacrylamide 5-25 part, epsilon-polylysine 5-10 part, matrine 3-5 part, calcium propionate 3-5 part, xitix 3-5 part, EDTA3-5 part, potassium fulvate 5-10 part, ZnCl
23-10 part and microbiobacterial agent 50-150 part, all the other are water.
5. the preparation method of a kind of apple tree fertilizer as claimed in claim 4, is characterized in that, step is:
(1) obtain respectively the fermented liquid of each bacterial classification after the bacterial classification in complex micro organism fungicide is fermented separately respectively, then the fermented liquid of single culture is mixed to ferment obtaining complex micro organism fungicide;
(2) by polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mix, stir;
(3) complex micro organism fungicide obtained in step (1) is fully mixed to thin up with the mixture in step (2).
6. the preparation method of a kind of apple tree fertilizer according to claim 5, it is characterized in that, in step (1), the bacterial classification in complex micro organism fungicide carries out the single culture fermentation and adopts one or more in beef-protein medium, potato culture, LB substratum.
7. the preparation method of a kind of apple tree fertilizer according to claim 6, it is characterized in that, after in step (1), the bacterial classification in complex micro organism fungicide being fermented separately respectively, inoculum size by the fermented liquid of single culture by 1-3% all is inoculated in the mixed strains substratum, wherein, the mixed strains substratum contains polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture aqueous solution, fermented, obtain complex micro organism fungicide.
8. the preparation method of a kind of apple tree fertilizer according to claim 7, is characterized in that, in step (1), and in the mixed strains substratum, polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture and the mass ratio of water be 2-5:100.
9. the preparation method of a kind of apple tree fertilizer according to claim 8, is characterized in that, in the mixed strains substratum, and polyacrylamide, epsilon-polylysine, matrine, calcium propionate, xitix, EDTA, potassium fulvate, ZnCl
2mixture aqueous solution in add the zinc sulfate that massfraction is 0.3%-1%.
10. the preparation method of a kind of apple tree fertilizer according to claim 5, it is characterized in that, in step (3), the complex micro organism fungicide obtained in step (1) is fully mixed with the mixture in step (2), and weight ratio is polyacrylamide 5-25 part, epsilon-polylysine 5-10 part, matrine 3-5 part, calcium propionate 3-5 part, xitix 3-5 part, EDTA3-5 part, potassium fulvate 5-10 part, ZnCl
23-10 part and microbiobacterial agent 50-150 part, after thin up, the zinc sulfate that the interpolation massfraction is 0.3%-1%.
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| CN104355774A (en) * | 2014-11-17 | 2015-02-18 | 砀山县沃福农业发展有限公司 | Special organic-inorganic compound fertilizer for apples |
| CN104876722A (en) * | 2015-05-19 | 2015-09-02 | 潍坊友容实业有限公司 | Sophocarpidine-containing liquid fertilizer applicable to saline-alkali soil and application of liquid fertilizer to plantation of Zhongning wolfberry trees |
| CN106431596A (en) * | 2016-06-29 | 2017-02-22 | 李怀峰 | Biological stimulant |
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| CN104876722A (en) * | 2015-05-19 | 2015-09-02 | 潍坊友容实业有限公司 | Sophocarpidine-containing liquid fertilizer applicable to saline-alkali soil and application of liquid fertilizer to plantation of Zhongning wolfberry trees |
| CN106431596A (en) * | 2016-06-29 | 2017-02-22 | 李怀峰 | Biological stimulant |
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| Publication number | Publication date |
|---|---|
| CN103483055B (en) | 2014-12-10 |
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