CN103478724B - Superworm powder rich in organic chromium, and preparation method thereof - Google Patents
Superworm powder rich in organic chromium, and preparation method thereof Download PDFInfo
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- CN103478724B CN103478724B CN201310472417.3A CN201310472417A CN103478724B CN 103478724 B CN103478724 B CN 103478724B CN 201310472417 A CN201310472417 A CN 201310472417A CN 103478724 B CN103478724 B CN 103478724B
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- larva
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- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 title claims abstract description 123
- 239000011651 chromium Substances 0.000 title claims abstract description 122
- 229910052804 chromium Inorganic materials 0.000 title claims abstract description 119
- 239000000843 powder Substances 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 241000255737 Zophobas atratus Species 0.000 title abstract 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 10
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 239000008367 deionised water Substances 0.000 claims abstract description 4
- 240000005979 Hordeum vulgare Species 0.000 claims description 58
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 58
- 229940046374 chromium picolinate Drugs 0.000 claims description 21
- GJYSUGXFENSLOO-UHFFFAOYSA-N chromium;pyridine-2-carboxylic acid Chemical compound [Cr].OC(=O)C1=CC=CC=N1.OC(=O)C1=CC=CC=N1.OC(=O)C1=CC=CC=N1 GJYSUGXFENSLOO-UHFFFAOYSA-N 0.000 claims description 20
- 239000001055 blue pigment Substances 0.000 claims description 14
- 239000010941 cobalt Substances 0.000 claims description 14
- 229910017052 cobalt Inorganic materials 0.000 claims description 14
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 10
- 238000009395 breeding Methods 0.000 claims description 8
- 230000001488 breeding effect Effects 0.000 claims description 8
- 235000015099 wheat brans Nutrition 0.000 claims description 8
- 238000004108 freeze drying Methods 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 6
- 230000008014 freezing Effects 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims description 4
- 230000003442 weekly effect Effects 0.000 claims description 4
- CAXNYFPECZCGFK-UHFFFAOYSA-N 2-phenyl-2-pyridin-2-ylacetonitrile Chemical compound C=1C=CC=NC=1C(C#N)C1=CC=CC=C1 CAXNYFPECZCGFK-UHFFFAOYSA-N 0.000 claims description 3
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 3
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims description 3
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims description 3
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000010419 fine particle Substances 0.000 claims description 3
- 229940014144 folate Drugs 0.000 claims description 3
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 3
- 235000019152 folic acid Nutrition 0.000 claims description 3
- 239000011724 folic acid Substances 0.000 claims description 3
- 239000010720 hydraulic oil Substances 0.000 claims description 3
- 238000012792 lyophilization process Methods 0.000 claims description 3
- 229940049920 malate Drugs 0.000 claims description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000004513 sizing Methods 0.000 claims description 3
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 claims description 3
- 244000024893 Amaranthus tricolor Species 0.000 claims description 2
- 235000014748 Amaranthus tricolor Nutrition 0.000 claims description 2
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 claims description 2
- 235000005602 Coleus parviflorus Nutrition 0.000 claims description 2
- 244000096857 Dioscorea batatas Species 0.000 claims description 2
- 235000002722 Dioscorea batatas Nutrition 0.000 claims description 2
- 241000222336 Ganoderma Species 0.000 claims description 2
- 241000318836 Pleurotus nebrodensis Species 0.000 claims description 2
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 claims description 2
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 claims description 2
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 claims description 2
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 claims description 2
- 235000005493 rutin Nutrition 0.000 claims description 2
- 229960004555 rutoside Drugs 0.000 claims description 2
- 229960003080 taurine Drugs 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 6
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 229920002101 Chitin Polymers 0.000 abstract description 3
- 238000004925 denaturation Methods 0.000 abstract description 3
- 230000036425 denaturation Effects 0.000 abstract description 3
- 206010012601 diabetes mellitus Diseases 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 229910021641 deionized water Inorganic materials 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 235000004213 low-fat Nutrition 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 235000001014 amino acid Nutrition 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 241000254109 Tenebrio molitor Species 0.000 description 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 2
- 108090001061 Insulin Proteins 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 241001124191 Tenebrio obscurus Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000023852 carbohydrate metabolic process Effects 0.000 description 2
- BFGKITSFLPAWGI-UHFFFAOYSA-N chromium(3+) Chemical compound [Cr+3] BFGKITSFLPAWGI-UHFFFAOYSA-N 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- HEQBUZNAOJCRSL-UHFFFAOYSA-N iron(ii) chromite Chemical compound [O-2].[O-2].[O-2].[Cr+3].[Fe+3] HEQBUZNAOJCRSL-UHFFFAOYSA-N 0.000 description 2
- MOUPNEIJQCETIW-UHFFFAOYSA-N lead chromate Chemical compound [Pb+2].[O-][Cr]([O-])(=O)=O MOUPNEIJQCETIW-UHFFFAOYSA-N 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000013110 organic ligand Substances 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- NNGFUSPIRIORIH-UHFFFAOYSA-H [Cr+3].[Cr+3].[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1 Chemical compound [Cr+3].[Cr+3].[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1 NNGFUSPIRIORIH-UHFFFAOYSA-H 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229910001430 chromium ion Inorganic materials 0.000 description 1
- QOWZHEWZFLTYQP-UHFFFAOYSA-K chromium(3+);triformate Chemical compound [Cr+3].[O-]C=O.[O-]C=O.[O-]C=O QOWZHEWZFLTYQP-UHFFFAOYSA-K 0.000 description 1
- HPCCGRCEBFBZQP-UHFFFAOYSA-N chromium;pyridine-3-carboxylic acid Chemical compound [Cr].OC(=O)C1=CC=CN=C1 HPCCGRCEBFBZQP-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 231100000025 genetic toxicology Toxicity 0.000 description 1
- 230000001738 genotoxic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 239000010438 granite Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000003345 hyperglycaemic effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention relates to a superworm powder rich in organic chromium, and a preparation method thereof. The content of organic trivalent chromium in the superworm powder rich in organic chromium is 10-200mg/kg. The preparation method of the superworm powder comprises the following steps: cleaning superworm larvae rich in organic chromium with deionized water, lyophilizing, carrying out low temperature squeezing, crushing, and carrying out ultramicro crushing. The superworm powder prepared in the invention well reserves the conformation of organic complex chromium, is rich in nutrients comprising free amino acids, water-soluble proteins, chitins and the like, and has a low protein denaturation degree, the granularity of crushed nutrients is extremely small, so it is benefit for the digestion and absorption; and the superworm powder has a low fat content, so the superworm powder has substantial healthcare effects on special people comprising fat people, diabetic patients and the like.
Description
Technical field
The present invention relates to a kind of barley worm worm powder and preparation method thereof, particularly one rich Organic Chromium barley worm worm powder and preparation method thereof.
Background technology
Chromium (III) is one of trace element of wanting of body weight for humans, and has function of blood sugar reduction.When lacking chromium in body, insulin level declines, and brings out glycometabolism, disorders of lipid metabolism.Research finds to supplement the sensitiveness that chromium can strengthen insulin, regulates glycometabolism and lipid metaboli.Trivalent chromic ion is the active component of GTF (GTF, Glucose Tolerance Factor).GTF is with nicotinic acid-trivalent chromium-nicotinic acid for axle center, and glutamic acid, glycine and cysteine are the material of part.Simple chromium ion does not have BA, and the chromium in animal body is nearly all trivalent chromium, but the function of its synthesis GTF is very poor, and the inorganic chromium supplemented is (as CrCl
3) very slow to the transformation of GTF, as compared to inorganic chromium (absorptivity is very low, 0.4%-3%), the chromium of GTF form is more easily absorbed (absorptivity is 10%-25%), and has higher biology utilization rate.Because GTF prepares difficulty comparatively greatly, therefore, the functional component of the auxiliary hyperglycemic function food be commercially most widely used at present and nutrient prime replenisher are chromium picolinate, the chromium folate of Prof. Du Yucang, the simple Organic Chromium preparation such as stachyose chromium.But due to the toxic and side effect of different Organic Chromium products and biological value difference very large, report is had to confirm, Organic Chromium-chromium picolinate most widely used in the market can produce DNA toxicity, cytotoxicity and genotoxicity, and its security is paid close attention to widely.Therefore, the research of the novel Organic Chromium complex that hypoglycemic activity is good, security is high is still one of current important topic.
The yeast chromium utilizing brewer's yeast to produce is the Organic Chromium after biology transforms that sales volume is the highest in the market.Yeast cells has the biological ability transformed, the chromium added be not physical absorption at cell surface, but there occurs organically biological conversion.But testing result shows, and still has the chromium more than 15% to exist with the form of inorganic chromium in Cr-enriched yeast, if still there is certain security risk in a large amount of this based article edible.
The insect of barley Chong Shi TRenebrionidae, being China Novel cultivation insect newly developed, is protein sources and the grease source of very high-quality.Barley worm larva polypide has the ability of higher orienting enriching to natural trace element.The present invention intends utilizing insect to carry out further biological conversion to commercially available Chromium Picolinate, trivalent chromium is combined with the amino acid in insect bodies, defines the form being easy to absorption of human body most.In addition, through freeze drying, cold pressing, insect powder after ultramicro grinding, not only wherein the conformation of organic cooperation chromium can well be retained, and is wherein also rich in the nutriments such as free amino acid, water soluble protein, chitin, wherein protein denaturation degree is low, after pulverizing, the granularity of various nutriment is minimum, is more conducive to digest and assimilate, has significant health-care effect to diabetes patient.
Summary of the invention
In view of this, main purpose of the present invention is to provide the rich Organic Chromium barley worm worm powder and preparation method thereof of a kind of bioavailability higher than existing market product.
For achieving the above object, the invention provides a kind of preparation method of rich Organic Chromium barley worm worm powder, comprising the following steps:
1) clean: after rich Organic Chromium barley larva is pressed the long sizing screening of body, respectively by the washed with de-ionized water 2-5min of the worm alive of different brackets through 80-95 DEG C, to remove dirt and to make barley worm larva not creep;
2) freeze drying: the rich Organic Chromium barley worm larva after cleaning is laid on pallet according to the thickness of 1-5cm, then freezing at putting into-20-80 DEG C, after freezing 1-2 hour, taking-up is put into freeze drier and is carried out lyophilization process 20-40 hour, the water content of the barley worm larva after drying and dehydrating is 3%-5%, and stores at the temperature of 0-5 DEG C after utilizing plastic sealing bag to seal up for safekeeping;
3) cold pressing:
3.1) preheat: the conveyer belt that the rich Organic Chromium barley worm larva stored is laid in microwave dryer is carried out preheating 2-7 minute in 50-80 DEG C;
3.2) hydraulic pressure squeezing: the rich Organic Chromium barley worm larva after preheating is packed into rapidly in the cavity of hydraulic oil press, pressurised extraction 2-15min;
4) ultramicro grinding: the de-oiling polypide squeezed by hydraulic pressure in rear chamber is taken out and leaves standstill, treat that temperature is down to normal temperature, first be made into 5-10 object fine particle with pulverizer, put it into rolling wheel rotating speed is 350-480 rev/min, cyclonic wind unit frequency is 5-40HZ frequency modulation afterwards again to roll in Cyclonic micronizer and carry out superfine comminution, to make 400-500 object ultra micro worm powder.
Preferably, wherein step 1) described rich Organic Chromium barley larva is classified as two-stage 10-12cm and 12-15cm by body is long.
Preferably, wherein said cultivation rich Organic Chromium barley worm larva is obtained by following concrete steps:
1) after the barley worm larva worm of cultivation reaches 60 ages in days age, start rich chromium feed of feeding, after cultivating 4 weeks, namely obtain described rich Organic Chromium barley worm larva; Its concrete cultivating condition is as follows: cultivation temperature is 25 DEG C-27 DEG C, and relative humidity is 65-75%, and whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20-40W irradiates, and irradiation time is 8 hours/day; Cultivation density: larva cultivation is in breeding box, and wherein the density of larva is 4-6 head/cm
2;
2) feedstuff feeding: adopt based on fine fodder, cobalt blue pigment is that auxiliary feed mode is fed, concentrated feed is thrown in weekly once, inventory is the 20-40% that worm weighs, wherein, the fine fodder thrown in for 1st week is be the above rich chromium feed and wheat bran of 1:1 by weight percentage, and the 2nd week-fine fodder of 4 weeks is the above rich chromium feed; Cobalt blue pigment is thrown in once for every two days, and inventory is the 10-30% that worm weighs.
Preferably, in wherein said rich chromium feed, organic chromic content is 10mg/kg-200mg/kg.
Preferably, the raw material of wherein said rich chromium feed also containing following percentage by weight:
Wheat bran: 50-70%; Bacterium chaff: 0-30%; Dregs of beans: 0-10%; Corn protein powder: 0-3%; Soyabean protein powder: 0-3%; Moisture: 5-15%.
Preferably, wherein said bacterium chaff is the excellent bacterium chaff pulverizing the Fluorine-rich granite magma such as the rear rich chromium Pleurotus nebrodensis, rich chromium mushroom, rich chromium flat mushroom or the Ganoderma rich in chromium that obtain of bacterium once or after secondary fruiting.
Preferably, wherein said rich chromium feed is that primary raw material is formulated with Chromium Picolinate, and described Chromium Picolinate is commercially available adjoin pyridine chromic formate, chromium malate, rutin chromium, chromium folate, stachyose chromium, taurine chromium or Cr-enriched yeast powder.
Preferably, wherein said cobalt blue pigment is the cauline leaf of commercially available Chinese cabbage, potato or spinach; Described fine fodder and cobalt blue pigment ratio is by weight percentage 1:2.
The present invention further provides by the obtained rich Organic Chromium barley worm worm powder of said method.
Preferably, in wherein said rich Organic Chromium barley worm worm powder, final organic chromic content is 10mg/kg-100mg/kg (after organic trivalent chromium content reaches balance in 2-4 week barley worm polypide).
The above-mentioned method preparing rich chromium barley worm worm powder of the present invention, also can be widely used in aspects such as producing rich chrome yellow mealworm worm powder, rich chrome black mealworm worm powder or rich chromium brown mealworm worm powder.
Those skilled in the art can by conjunction with prior art or common practise, add rich selenium edible fungi residue feed in yellow meal worm, dark mealworm, brown mealworm after, by different rate of charges, feed yellow meal worm, dark mealworm, brown mealworm etc., obtain the rich chrome yellow mealworm of chrome content 10-200mg/kg, rich chrome black mealworm or the brown mealworm of rich chromium etc.
The present invention has following beneficial effect:
1, first the chrome additive used in the breeding process of barley worm is Chromium Picolinate, except ensure not have in product inorganic chromium residual except, insect can also be made to have higher absorption rate to it, thus obtain the higher barley worm commercial insect (Organic Chromium content 10-200mg/kg) of chrome content, and chromium element is accumulated in through Long-term Feeding in insect bodies, so be all with in animal body can stable existence organic ligand form exist, therefore the various toxic and side effects that inorganic chromium and Chromium Picolinate may exist are completely eliminated, edible safety is very high,
2, by barley worm, the biology of Chromium Picolinate is transformed, chromium is combined with the amino acid in insect bodies, defining the stable organic ligand being easy to absorption of human body most, chromium more efficiently, is more safely absorbed and used, is the Organic Chromium replenishers with stronger competitive advantage;
3, through freeze drying, cold pressing, barley worm worm powder after ultramicro grinding, not only wherein the conformation of organic cooperation chromium can well be retained, and be wherein also rich in the nutriments such as free amino acid, water soluble protein, chitin, wherein protein denaturation degree is low, after pulverizing, the granularity of various nutriment is minimum, be more conducive to digest and assimilate, and fat content is lower, to the special population such as obese people, diabetes patient, there is significant health-care effect.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described.
Following reagent, without special instruction, is commercially available.
Embodiment 1
The formula (by weight percentage) of rich chromium feed:
Wheat bran: 70%; Bacterium chaff: 10%; Dregs of beans: 5%; Corn protein powder: 4.9%; Moisture: 10%; Chromium picolinate: 0.1%.
Cultivate the method for rich chromium barley worm larva (containing organic trivalent chromium 80-120mg/kg), comprise the following steps:
After the barley worm larva worm of cultivation reaches 60 ages in days age, start rich chromium feed of feeding, after cultivating 4 weeks, namely obtain described rich Organic Chromium barley worm larva (ripe commercial insect).Its concrete cultural method is as follows:
Breeding environment: cultivation temperature is 25 DEG C-27 DEG C, and relative humidity is 65-75%, whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20W irradiates, and irradiation time is 8 hours/day.
Cultivation density: larva cultivation is in breeding box (material is wooden, non-toxic plastic or papery, and size is not limit), and wherein the density of larva is 4-6 head/cm
2.
Feedstuff feeding: adopt based on fine fodder, cobalt blue pigment is that auxiliary feed mode is fed, the ratio of fine fodder and cobalt blue pigment is 1:2 (wt)), concentrated feed is thrown in weekly once, inventory is the 25-30% that worm weighs, wherein, the concentrated feed thrown in for 1st week is the above rich chromium feed and wheat bran of 1:1 by weight percentage, and the 2nd week-concentrated feed of 4 weeks is the above rich chromium feed; Described cobalt blue pigment can be commercially available Chinese cabbage, within every two days, throws in once, and inventory is the 10-15% that worm weighs.
Embodiment 2
The formula (by weight percentage) of rich chromium feed:
Wheat bran: 68%; Bacterium chaff: 10%; Dregs of beans: 7%; Soyabean protein powder: 4.8%; Moisture: 10%; Chromium malate: 0.2%.
Cultivate the method for rich chromium barley worm larva (containing organic trivalent chromium 150-200mg/kg), comprise the following steps:
After the barley worm larva worm of cultivation reaches 60 ages in days age, after cultivating 4 weeks, namely obtain described rich Organic Chromium barley worm larva (ripe commercial insect).Its concrete cultural method is as follows:
Breeding environment: cultivation temperature is 25 DEG C-27 DEG C, and relative humidity is 65-75%, whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20W irradiates, and irradiation time is 8 hours/day.
Cultivation density: larva cultivation is in breeding box (material is wooden, non-toxic plastic or papery, and size is not limit), and wherein the density of larva is 4-6 head/cm
2.
Feedstuff feeding: adopt based on fine fodder, cobalt blue pigment is that auxiliary feed mode is fed, the ratio of fine fodder and cobalt blue pigment is 1:2 (wt)), concentrated feed is thrown in weekly once, inventory is the 25-30% that worm weighs, wherein, the above rich chromium feed and wheat bran of the concentrated feed 1:1 by weight percentage thrown in for 1st week, the 2nd week-concentrated feed of 4 weeks is the above rich chromium feed; Described cobalt blue pigment can be commercially available potato, within every two days, throws in once, and inventory is the 10-15% that worm weighs.
Embodiment 3
The preparation method of rich Organic Chromium barley worm worm powder, comprises the following steps:
1) clean: rich Organic Chromium barley worm larva embodiment 1 cultivated (is classified as two-stage 10-12cm and 12-15cm by body is long by the long sizing screening of body, so that follow-up freeze drying step) after, respectively by the washed with de-ionized water 3min of the worm alive of different brackets through 90 DEG C, to remove dirt and to make barley worm larva not creep;
2) freeze drying: the rich Organic Chromium barley worm larva after cleaning is laid on pallet (diameter 20-40cm) according to the thickness of 3cm, then freezing at putting into-20 DEG C, after freezing 1 hour, taking-up is put into freeze drier and is carried out lyophilization process 24 hours, the water content of the barley worm larva after drying and dehydrating is 4%, and stores at the temperature of 0 DEG C after utilizing plastic sealing bag to seal up for safekeeping;
3) cold pressing:
3.1) preheat: the conveyer belt barley worm larva of storage being laid in microwave dryer carries out preheating 5 minutes in 60 DEG C;
3.2) hydraulic pressure squeezing: the barley worm larva after preheating is packed into rapidly in the cavity of hydraulic oil press, pressurised extraction 10min;
4) ultramicro grinding: the de-oiling polypide squeezed by hydraulic pressure in rear chamber is taken out and leaves standstill, treat that temperature is down to normal temperature, first be made into 10 object fine particles with pulverizer, put it into rolling wheel rotating speed is 350-480 rev/min, cyclonic wind unit frequency is 5-40HZ frequency modulation afterwards again to roll in Cyclonic micronizer and carry out superfine comminution, to make 500 object ultra micro worm powder.
Embodiment 4 testing experiment:
The barley worm larva of 60 ages in days of Chrome-free feed fed by heavy for initial worm consistent 1800g is equally divided into 18 boxes, and be divided into blank test group, low chromium picolinate test group, high chromium picolinate test group, often organize six repetitions (6 box barley worm), 18 box barley worms are 25 DEG C-27 DEG C in temperature, relative humidity 65-75%, whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20W irradiates, irradiation time is cultivate under condition for 8 hours/day, and in breeding box, the density of larva is 4-6 head/cm
2, cultivate after 28 days and the cooperation Organic Chromium in test polypide and chromium picolinate content detected.
Data processing Using statistics software SPSS for Windows l9.0, adopts one-way analysis of variance between process, and after statistical discrepancy is remarkable, carry out significance test with the biological statistical method of Duncans Multiple range test, the level of signifiance is set to 0.05.Concrete outcome is as shown in table 1 below:
The rich chromium comparative test result of table 1 barley worm
| Test group | Containing the amount wt% of chromium picolinate in feed | Average worm is heavy g/ afterwards to cultivate 28 days | Average amino acid coordinates Organic Chromium content mg/kg | Average chromium picolinate content mg/kg |
| Blank group | 0 | 0.782a | 18.01±1.11a | 0.00±0.00a |
| Low chromium feed group | 0.1 | 0.790a | 85.27±3.25b | 0.52±0.02b |
| High chromium feed group | 0.2 | 0.779a | 143.25±4.51c | 0.56±0.13b |
As can be seen from the result of the contrast test of table 1, when adding chromium picolinate 0.1wt% in feed, the speed of growth of barley worm is not suppressed, and Organic Chromium content is apparently higher than control group; When adding chromium picolinate 0.2wt% in feed, the speed of growth of barley worm is not suppressed, and Organic Chromium content is also significantly increased, but concentration effect declines to some extent.As can be seen here, the rich chromium feed utilizing the embodiment of the present invention 1 to prepare is fed barley worm larva, do not affect the growth traits of barley worm larva, but the Organic Chromium content of barley worm larva can be significantly improved, and wherein Chromium Picolinate residual quantity is extremely low, therefore edible safety is high, with the obvious advantage compared with existing Organic Chromium replenishers in the market.
According to the metabolism research display in animal body, all become fit form to exist with amino acid in animal body after chromium is absorbed and used, therefore only need detect the chromium wherein having the original interpolation forms how much do not transformed, then be amino acid cooperation chromium with the chromium picolinate that total chromium deducts interpolation.The additive added due to the present invention is trivalent Organic Chromium, after barley worm (insect) metabolism, therefore there is not the possibility changing inorganic chromium into.
The above embodiment is only that protection scope of the present invention is not limited thereto in order to absolutely prove the preferred embodiment that the present invention enumerates.The equivalent alternative or conversion that those skilled in the art do on basis of the present invention, all within protection scope of the present invention.
Claims (6)
1. a preparation method for rich Organic Chromium barley worm worm powder, is characterized in that: comprise the following steps:
1) after the barley worm larva worm of cultivation reaches 60 ages in days age, start rich chromium feed of feeding, after cultivating 4 weeks, namely obtain described rich Organic Chromium barley worm larva; Its concrete cultivating condition is as follows: cultivation temperature is 25 DEG C-27 DEG C, and relative humidity is 65-75%, and whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20-40W irradiates, and irradiation time is 8 hours/day; Cultivation density: larva cultivation is in breeding box, and wherein the density of larva is 4-6 head/cm
2;
2) feedstuff feeding: adopt based on fine fodder, cobalt blue pigment is that auxiliary feed mode is fed, concentrated feed is thrown in weekly once, inventory is the 20-40% that worm weighs, wherein, the fine fodder thrown in for 1st week is be the above rich chromium feed and wheat bran of 1:1 by weight percentage, and the 2nd week-fine fodder of 4 weeks is the above rich chromium feed; Cobalt blue pigment is thrown in once for every two days, and inventory is the 10-30% that worm weighs;
3) clean: by above-mentioned steps 2) after the rich Organic Chromium barley larva that obtains is two-stage 10-12cm and 12-15cm by the long sizing screening of body, respectively by the washed with de-ionized water 2-5min of the worm alive of different brackets through 80-95 DEG C, to remove dirt and to make barley worm larva not creep;
4) freeze drying: the rich Organic Chromium barley worm larva after cleaning is laid on pallet according to the thickness of 1-5cm, then freezing at putting into-20--80 DEG C, after freezing 1-2 hour, taking-up is put into freeze drier and is carried out lyophilization process 20-40 hour, the water content of the barley worm larva after drying and dehydrating is 3%-5%, and stores at the temperature of 0-5 DEG C after utilizing plastic sealing bag to seal up for safekeeping;
5) cold pressing:
5.1) preheat: the conveyer belt that the rich Organic Chromium barley worm larva stored is laid in microwave dryer is carried out preheating 2-7 minute in 50-80 DEG C;
5.2) hydraulic pressure squeezing: the rich Organic Chromium barley worm larva after preheating is packed into rapidly in the cavity of hydraulic oil press, pressurised extraction 2-15min;
6) ultramicro grinding: the de-oiling polypide squeezed by hydraulic pressure in rear chamber is taken out and leaves standstill, treat that temperature is down to normal temperature, first be made into 5-10 object fine particle with pulverizer, put it into rolling wheel rotating speed is 350-480 rev/min, cyclonic wind unit frequency is 5-40HZ frequency modulation afterwards again to roll in Cyclonic micronizer and carry out superfine comminution, to make 400-500 object ultra micro worm powder;
Obtain described rich Organic Chromium barley worm worm powder by above-mentioned steps, its final organic chromic content is 10mg/kg-200mg/kg.
2. method according to claim 1; it is characterized in that: described rich chromium feed is that primary raw material is formulated with Chromium Picolinate, described Chromium Picolinate is commercially available chromium picolinate, chromium malate, rutin chromium, chromium folate, stachyose chromium, taurine chromium or Cr-enriched yeast powder.
3. method according to claim 2, is characterized in that: in described rich chromium feed, organic chromic content is 10mg/kg-200mg/kg.
4. method according to claim 2, is characterized in that: the raw material of described rich chromium feed also containing following percentage by weight:
Wheat bran: 50-70%; Bacterium chaff: 0-30%; Dregs of beans: 0-10%; Corn protein powder: 0-3%; Soyabean protein powder: 0-3%; Moisture: 5-15%.
5. method according to claim 4, is characterized in that: described bacterium chaff is the bacterium chaff of rich chromium Pleurotus nebrodensis, rich chromium mushroom, rich chromium flat mushroom or the Ganoderma rich in chromium obtained after the bacterium rod once or after secondary fruiting is pulverized.
6. method according to claim 1, is characterized in that: described cobalt blue pigment is the cauline leaf of commercially available Chinese cabbage, potato or spinach; Described fine fodder and cobalt blue pigment ratio is by weight percentage 1:2.
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| WO2016108037A1 (en) | 2014-12-31 | 2016-07-07 | Ynsect | Beetle powder |
| CN106174481A (en) * | 2016-07-08 | 2016-12-07 | 安徽济他倍乐生物科技有限公司 | A kind of preparation method of the Holotrichia diomphalia Bates dry powder of health food |
| CN112753884A (en) * | 2021-01-22 | 2021-05-07 | 广东绿金生物科技有限公司 | Feed additive formula for improving laying rate of laying poultry and improving quality of eggs |
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