CN103478724A - Superworm powder rich in organic chromium, and preparation method thereof - Google Patents
Superworm powder rich in organic chromium, and preparation method thereof Download PDFInfo
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- CN103478724A CN103478724A CN201310472417.3A CN201310472417A CN103478724A CN 103478724 A CN103478724 A CN 103478724A CN 201310472417 A CN201310472417 A CN 201310472417A CN 103478724 A CN103478724 A CN 103478724A
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- chromium
- rich
- worm
- barley
- powder
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- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 title claims abstract description 129
- 239000011651 chromium Substances 0.000 title claims abstract description 128
- 229910052804 chromium Inorganic materials 0.000 title claims abstract description 125
- 239000000843 powder Substances 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 241000255737 Zophobas atratus Species 0.000 title abstract 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 10
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims abstract description 4
- 239000008367 deionised water Substances 0.000 claims abstract description 4
- 240000005979 Hordeum vulgare Species 0.000 claims description 63
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 63
- 229940046374 chromium picolinate Drugs 0.000 claims description 21
- GJYSUGXFENSLOO-UHFFFAOYSA-N chromium;pyridine-2-carboxylic acid Chemical compound [Cr].OC(=O)C1=CC=CC=N1.OC(=O)C1=CC=CC=N1.OC(=O)C1=CC=CC=N1 GJYSUGXFENSLOO-UHFFFAOYSA-N 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 16
- 239000001055 blue pigment Substances 0.000 claims description 14
- 239000010941 cobalt Substances 0.000 claims description 14
- 229910017052 cobalt Inorganic materials 0.000 claims description 14
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 14
- 241000894006 Bacteria Species 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 9
- 238000009395 breeding Methods 0.000 claims description 8
- 230000001488 breeding effect Effects 0.000 claims description 8
- 235000015099 wheat brans Nutrition 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 7
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 6
- 230000008014 freezing Effects 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims description 4
- 230000003442 weekly effect Effects 0.000 claims description 4
- CAXNYFPECZCGFK-UHFFFAOYSA-N 2-phenyl-2-pyridin-2-ylacetonitrile Chemical compound C=1C=CC=NC=1C(C#N)C1=CC=CC=C1 CAXNYFPECZCGFK-UHFFFAOYSA-N 0.000 claims description 3
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 3
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims description 3
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims description 3
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000010419 fine particle Substances 0.000 claims description 3
- 235000019152 folic acid Nutrition 0.000 claims description 3
- 239000010720 hydraulic oil Substances 0.000 claims description 3
- 229940049920 malate Drugs 0.000 claims description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000004513 sizing Methods 0.000 claims description 3
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 claims description 3
- 244000024893 Amaranthus tricolor Species 0.000 claims description 2
- 235000014748 Amaranthus tricolor Nutrition 0.000 claims description 2
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 claims description 2
- 235000005602 Coleus parviflorus Nutrition 0.000 claims description 2
- 244000096857 Dioscorea batatas Species 0.000 claims description 2
- 235000002722 Dioscorea batatas Nutrition 0.000 claims description 2
- 241000222336 Ganoderma Species 0.000 claims description 2
- 241000318836 Pleurotus nebrodensis Species 0.000 claims description 2
- QOWZHEWZFLTYQP-UHFFFAOYSA-K chromium(3+);triformate Chemical compound [Cr+3].[O-]C=O.[O-]C=O.[O-]C=O QOWZHEWZFLTYQP-UHFFFAOYSA-K 0.000 claims description 2
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 claims description 2
- 229940014144 folate Drugs 0.000 claims description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 2
- 239000011724 folic acid Substances 0.000 claims description 2
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims description 2
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 claims description 2
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 claims description 2
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 claims description 2
- 235000005493 rutin Nutrition 0.000 claims description 2
- 229960004555 rutoside Drugs 0.000 claims description 2
- 229960003080 taurine Drugs 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 6
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 229920002101 Chitin Polymers 0.000 abstract description 3
- 238000004925 denaturation Methods 0.000 abstract description 3
- 230000036425 denaturation Effects 0.000 abstract description 3
- 206010012601 diabetes mellitus Diseases 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 229910021641 deionized water Inorganic materials 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 235000004213 low-fat Nutrition 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 12
- 238000012360 testing method Methods 0.000 description 10
- 235000001014 amino acid Nutrition 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- 230000036983 biotransformation Effects 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 241000254109 Tenebrio molitor Species 0.000 description 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 2
- 108090001061 Insulin Proteins 0.000 description 2
- 241001124191 Tenebrio obscurus Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000023852 carbohydrate metabolic process Effects 0.000 description 2
- BFGKITSFLPAWGI-UHFFFAOYSA-N chromium(3+) Chemical compound [Cr+3] BFGKITSFLPAWGI-UHFFFAOYSA-N 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- HEQBUZNAOJCRSL-UHFFFAOYSA-N iron(ii) chromite Chemical compound [O-2].[O-2].[O-2].[Cr+3].[Fe+3] HEQBUZNAOJCRSL-UHFFFAOYSA-N 0.000 description 2
- MOUPNEIJQCETIW-UHFFFAOYSA-N lead chromate Chemical compound [Pb+2].[O-][Cr]([O-])(=O)=O MOUPNEIJQCETIW-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000013110 organic ligand Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- NNGFUSPIRIORIH-UHFFFAOYSA-H [Cr+3].[Cr+3].[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1 Chemical compound [Cr+3].[Cr+3].[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1.[O-]C(=O)c1ccccn1 NNGFUSPIRIORIH-UHFFFAOYSA-H 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- -1 chromium folates Chemical class 0.000 description 1
- 229910001430 chromium ion Inorganic materials 0.000 description 1
- HPCCGRCEBFBZQP-UHFFFAOYSA-N chromium;pyridine-3-carboxylic acid Chemical compound [Cr].OC(=O)C1=CC=CN=C1 HPCCGRCEBFBZQP-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 231100000025 genetic toxicology Toxicity 0.000 description 1
- 230000001738 genotoxic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000003345 hyperglycaemic effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to a superworm powder rich in organic chromium, and a preparation method thereof. The content of organic trivalent chromium in the superworm powder rich in organic chromium is 10-200mg/kg. The preparation method of the superworm powder comprises the following steps: cleaning superworm larvae rich in organic chromium with deionized water, lyophilizing, carrying out low temperature squeezing, crushing, and carrying out ultramicro crushing. The superworm powder prepared in the invention well reserves the conformation of organic complex chromium, is rich in nutrients comprising free amino acids, water-soluble proteins, chitins and the like, and has a low protein denaturation degree, the granularity of crushed nutrients is extremely small, so it is benefit for the digestion and absorption; and the superworm powder has a low fat content, so the superworm powder has substantial healthcare effects on special people comprising fat people, diabetic patients and the like.
Description
Technical field
The present invention relates to a kind of barley worm worm powder and preparation method thereof, particularly a kind of rich Organic Chromium barley worm worm powder and preparation method thereof.
Background technology
Chromium (III) is one of trace element of wanting of body weight for humans, and has function of blood sugar reduction.While in body, lacking chromium, insulin level descends, and brings out glycometabolism, disorders of lipid metabolism.Research finds to supplement the sensitiveness that chromium can strengthen insulin, regulates glycometabolism and fat metabolism.Trivalent chromic ion is the active component of GTF (GTF, Glucose Tolerance Factor).GTF be take nicotinic acid-trivalent chromium-nicotinic acid as axle center, the material that glutamic acid, glycine and cysteine are part.Simple chromium ion does not have BA, and the chromium in animal body is nearly all trivalent chromium, but the function of its synthetic GTF is very poor, and supplementary inorganic chromium is (as CrCl
3) very slow to the transformation of GTF, with inorganic chromium, (absorptivity is very low, 0.4%-3%) compares, and the chromium of GTF form more easily is absorbed (absorptivity is 10%-25%), and has higher biology utilization rate.Due to GTF, to prepare difficulty larger, and therefore, the functional component of the auxiliary hyperglycemic function food be most widely used on market at present and nutrient prime replenisher are artificial synthetic chromium picolinate, chromium folates, the simple Organic Chromium preparation such as stachyose chromium.But because toxic and side effect and the biological value difference of different Organic Chromium products are very large, there is report to confirm, most widely used Organic Chromium-chromium picolinate can produce DNA toxicity, cytotoxicity and genotoxicity in the market, and its security is paid close attention to widely.The research of the novel Organic Chromium complex that therefore, hypoglycemic activity is good, safe is still one of current important topic.
The yeast chromium that utilizes brewer's yeast production is the highest Organic Chromium after bio-transformation of sales volume in the market.Yeast cells has the ability of bio-transformation, the chromium added be not physical absorption at cell surface, but organic bio-transformation has occurred.Yet testing result shows, in Cr-enriched yeast, still have the chromium that surpasses 15% to exist with the form of inorganic chromium, if still there is certain security risk in edible this based article in a large number.
The barley worm is the insect of TRenebrionidae, is China Novel cultivation insect newly developed, is protein sources and the grease source of very high-quality.Barley worm larva polypide has the ability of higher orienting enriching to natural trace element.The present invention intends utilizing insect to carry out further bio-transformation to commercially available Chromium Picolinate, and trivalent chromium is combined with the amino acid in insect bodies, has formed the form that is easy to absorption of human body most.In addition, through freeze drying, cold pressing, insect powder after ultramicro grinding, not only wherein the conformation of organic cooperation chromium can well be retained, and wherein also is rich in the nutriments such as free amino acid, water soluble protein, chitin, wherein the protein denaturation degree is low, after pulverizing, the granularity of various nutriments is minimum, is more conducive to digest and assimilate, and the diabetes patient is had to significant health-care effect.
Summary of the invention
In view of this, main purpose of the present invention is to provide a kind of bioavailability higher than rich Organic Chromium barley worm worm powder of existing market product and preparation method thereof.
For achieving the above object, the invention provides a kind of preparation method of rich Organic Chromium barley worm worm powder, comprise the following steps:
1) clean: after rich Organic Chromium barley larva is pressed to the long sizing screening of body, the washed with de-ionized water 2-5min through 80-95 ℃ by the worm alive of different brackets respectively, with the removal dirt and make barley worm larva not creep;
2) freeze drying: the thickness by the rich Organic Chromium barley worm larva after cleaning according to 1-5cm is laid on pallet, then freezing under put into-20-80 ℃, after freezing 1-2 hour, taking-up is put into freeze drier and is carried out lyophilization processing 20-40 hour, the water content of barley worm larva after drying and dehydrating is 3%-5%, and at the temperature of 0-5 ℃, stores after utilizing plastic sealing bag to seal up for safekeeping;
3) cold pressing:
3.1) preheat: the rich Organic Chromium barley worm larva stored is laid on the conveyer belt of microwave dryer and is preheated 2-7 minute in 50-80 ℃;
3.2) the hydraulic pressure squeezing: the rich Organic Chromium barley worm larva after preheating is packed into rapidly in the cavity of hydraulic oil press to pressurised extraction 2-15min;
4) ultramicro grinding: the de-oiling polypide that hydraulic pressure is squeezed in rear chamber is taken out standing, treat that temperature is down to normal temperature, first with pulverizer, be made into 5-10 purpose fine particle, the frequency modulation that to put it into afterwards the rolling wheel rotating speed again and be 350-480 rev/min, cyclonic wind unit frequency be 5-40HZ rolls in the Cyclonic micronizer and carries out ultra micro processing, to make 400-500 purpose ultra micro worm powder.
As preferred version, step 1 wherein) described rich Organic Chromium barley larva is classified as two-stage 10-12cm and 12-15cm by body length.
As preferred version, larva obtains by following concrete steps the rich Organic Chromium barley of wherein said cultivation worm:
1), after the barley worm larva worm of cultivation reaches 60 ages in days age, the rich chromium feed that starts to feed, obtain described rich Organic Chromium barley worm larva after cultivating 4 weeks; Its concrete cultivating condition is as follows: the cultivation temperature is 25 ℃-27 ℃, and relative humidity is 65-75%, and whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20-40W irradiates, and irradiation time is 8 hours/day; Cultivation density: larva cultivates in breeding box, and wherein the density of larva is 4-6 head/cm
2;
2) feedstuff feeding: adopt and take fine fodder and fed as main, cobalt blue pigment as the auxiliary mode of feeding, concentrated feed is thrown in weekly once, inventory is the heavy 20-40% of worm, wherein, the fine fodder of throwing in the 1st week is to be the above rich chromium feed and wheat bran of 1:1 by weight percentage, and the fine fodder of the 2nd week the-the 4th week is the above rich chromium feed; Cobalt blue pigment is thrown in once in every two days, and inventory is the heavy 10-30% of worm.
As preferred version, in wherein said rich chromium feed, organic chromic content is 10mg/kg-200mg/kg.
As preferred version, wherein said rich chromium feed also contains the raw material of following percentage by weight:
Wheat bran: 50-70%; Bacterium chaff: 0-30%; Dregs of beans: 0-10%; Corn protein powder: 0-3%; Soyabean protein powder: 0-3%; Moisture: 5-15%.
As preferred version, wherein said bacterium chaff is once or the bacterium chaff of the rich chromium edible mushrooms such as rich chromium Pleurotus nebrodensis, rich chromium mushroom, rich chromium flat mushroom or Ganoderma rich in chromium that obtain after pulverizing of the bacterium after secondary fruiting rod.
As preferred version, it is formulated as primary raw material that wherein said rich chromium feed be take Chromium Picolinate, and described Chromium Picolinate is commercially available pyridine chromic formate, chromium malate, rutin chromium, chromium folate, stachyose chromium, taurine chromium or the Cr-enriched yeast powder of adjoining.
As preferred version, the cauline leaf that wherein said cobalt blue pigment is commercially available Chinese cabbage, potato or spinach; Described fine fodder and cobalt blue pigment ratio by weight percentage is 1:2.
The present invention further provides the rich Organic Chromium barley worm worm powder made by said method.
As preferred version, in wherein said rich Organic Chromium barley worm worm powder, final organic chromic content is 10mg/kg-100mg/kg (after in 2-4 week barley worm polypide, organic trivalent chromium content reaches balance).
The above-mentioned method for preparing rich chromium barley worm worm powder of the present invention, also can be widely used in aspects such as producing rich chrome yellow mealworm worm powder, rich chrome black mealworm worm powder or the brown mealworm worm of rich chromium powder.
Those skilled in the art can pass through in conjunction with prior art or common practise, after adding rich selenium edible fungi residue feed in yellow meal worm, dark mealworm, brown mealworm, by different rate of charges, feed yellow meal worm, dark mealworm, brown mealworm etc., obtain the rich chrome yellow mealworm of chrome content 10-200mg/kg, rich chrome black mealworm or the brown mealworm of rich chromium etc.
The present invention has following beneficial effect:
1, at first the chrome additive used in the breeding process of barley worm is Chromium Picolinate, in guaranteeing product, do not have inorganic chromium residual, can also make insect there is higher absorption rate to it, thereby obtain the higher barley worm commercial insect (Organic Chromium content 10-200mg/kg) of chrome content, and the chromium element is accumulated in insect bodies through Long-term Feeding, so be all to exist with organic ligand form that in animal body can stable existence, therefore the various toxic and side effects that inorganic chromium and Chromium Picolinate may exist have thoroughly been eliminated, edible safety is very high,
2, the bio-transformation to Chromium Picolinate by the barley worm, chromium is combined with the amino acid in insect bodies, formed the stable organic ligand that is easy to absorption of human body most, make chromium can be more efficiently, more safely be absorbed and used, be the Organic Chromium replenishers with stronger competitive advantage;
3, through freeze drying, cold pressing, barley worm worm powder after ultramicro grinding, not only wherein the conformation of organic cooperation chromium can well be retained, and wherein also is rich in the nutriments such as free amino acid, water soluble protein, chitin, and wherein the protein denaturation degree is low, after pulverizing, the granularity of various nutriments is minimum, be more conducive to digest and assimilate, and fat content is lower, and the special populations such as obese people, diabetes patient are had to significant health-care effect.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Following reagent, without special instruction, is commercially available.
Embodiment 1
The formula (by weight percentage) of rich chromium feed:
Wheat bran: 70%; Bacterium chaff: 10%; Dregs of beans: 5%; Corn protein powder: 4.9%; Moisture: 10%; Chromium picolinate: 0.1%.
Cultivate the method for rich chromium barley worm larva (containing organic trivalent chromium 80-120mg/kg), comprise the following steps:
After the barley worm larva worm of cultivation reaches 60 ages in days age, the rich chromium feed that starts to feed, obtain described rich Organic Chromium barley worm larva (ripe commercial insect) after cultivating 4 weeks.Its concrete cultural method is as follows:
Breeding environment: the cultivation temperature is 25 ℃-27 ℃, and relative humidity is 65-75%, and whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20W irradiates, and irradiation time is 8 hours/day.
Cultivation density: larva cultivation is in breeding box (material is wooden, non-toxic plastic or papery, and size is not limit), and wherein the density of larva is 4-6 head/cm
2.
Feedstuff feeding: adopt and take fine fodder and fed as main, cobalt blue pigment as the auxiliary mode of feeding, the ratio of fine fodder and cobalt blue pigment is 1:2 (wt)), concentrated feed is thrown in weekly once, inventory is the heavy 25-30% of worm, wherein, the above rich chromium feed and wheat bran that the concentrated feed of throwing in the 1st week is 1:1 by weight percentage, the concentrated feed of the 2nd week the-the 4th week is the above rich chromium feed; Described cobalt blue pigment can be commercially available Chinese cabbage, within every two days, throws in once, and inventory is the heavy 10-15% of worm.
Embodiment 2
The formula (by weight percentage) of rich chromium feed:
Wheat bran: 68%; Bacterium chaff: 10%; Dregs of beans: 7%; Soyabean protein powder: 4.8%; Moisture: 10%; Chromium malate: 0.2%.
Cultivate the method for rich chromium barley worm larva (containing organic trivalent chromium 150-200mg/kg), comprise the following steps:
After the barley worm larva worm of cultivation reaches 60 ages in days age, obtain described rich Organic Chromium barley worm larva (ripe commercial insect) after cultivating 4 weeks.Its concrete cultural method is as follows:
Breeding environment: the cultivation temperature is 25 ℃-27 ℃, and relative humidity is 65-75%, and whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20W irradiates, and irradiation time is 8 hours/day.
Cultivation density: larva cultivation is in breeding box (material is wooden, non-toxic plastic or papery, and size is not limit), and wherein the density of larva is 4-6 head/cm
2.
Feedstuff feeding: adopt and take fine fodder and fed as main, cobalt blue pigment as the auxiliary mode of feeding, the ratio of fine fodder and cobalt blue pigment is 1:2 (wt)), concentrated feed is thrown in weekly once, inventory is the heavy 25-30% of worm, wherein, the concentrated feed of throwing in the 1st week is the above rich chromium feed and wheat bran of 1:1 by weight percentage, and the concentrated feed of the 2nd week the-the 4th week is the above rich chromium feed; Described cobalt blue pigment can be commercially available potato, within every two days, throws in once, and inventory is the heavy 10-15% of worm.
Embodiment 3
The preparation method of rich Organic Chromium barley worm worm powder comprises the following steps:
1) clean: the rich Organic Chromium barley worm larva of embodiment 1 cultivation (is classified as to two-stage 10-12cm and 12-15cm by body length by the long sizing screening of body, so that follow-up freeze drying step) after, the washed with de-ionized water 3min through 90 ℃ by the worm alive of different brackets respectively, to remove dirt and to make barley worm larva not creep;
2) freeze drying: the thickness by the rich Organic Chromium barley worm larva after cleaning according to 3cm is laid on pallet (diameter 20-40cm), then put under-20 ℃ freezing, after freezing 1 hour, taking-up is put into freeze drier and is carried out lyophilization processing 24 hours, the water content of barley worm larva after drying and dehydrating is 4%, and at the temperature of 0 ℃, stores after utilizing plastic sealing bag to seal up for safekeeping;
3) cold pressing:
3.1) preheat: the barley worm larva of storage is laid on the conveyer belt of microwave dryer and is preheated 5 minutes in 60 ℃;
3.2) the hydraulic pressure squeezing: the barley worm larva after preheating is packed into rapidly in the cavity of hydraulic oil press to pressurised extraction 10min;
4) ultramicro grinding: the de-oiling polypide that hydraulic pressure is squeezed in rear chamber is taken out standing, treat that temperature is down to normal temperature, first with pulverizer, be made into 10 purpose fine particles, the frequency modulation that to put it into afterwards the rolling wheel rotating speed again and be 350-480 rev/min, cyclonic wind unit frequency be 5-40HZ rolls in the Cyclonic micronizer and carries out ultra micro processing, to make 500 purpose ultra micro worm powder.
Embodiment 4 testing experiments:
The feed barley worm larva of 60 ages in days of Chrome-free feed of the heavy consistent 1800g of initial worm is equally divided into to 18 boxes, and it is divided into to blank test group, low chromium picolinate test group, high chromium picolinate test group, every group six repetitions (6 box barley worm), 18 box barley worms are 25 ℃-27 ℃ in temperature, relative humidity 65-75%, whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20W irradiates, irradiation time is under condition, to cultivate in 8 hours/day, and in breeding box, the density of larva is 4-6 head/cm
2, cultivate after 28 days cooperation Organic Chromium and chromium picolinate content in the test polypide detected.
Data process to be used statistic software SPSS for Windows l9.0, adopt one-way analysis of variance between processing, statistical discrepancy significantly after, by the Duncans multiple ratio, biological statistical method carries out significance test, the level of signifiance is made as 0.05.Concrete outcome is as shown in table 1 below:
The rich chromium comparative test result of table 1 barley worm
| Test group | Contain the amount wt% of chromium picolinate in feed | Average worm is heavy g/ afterwards to cultivate 28 days | Average amino acid coordinates Organic Chromium content mg/kg | Average chromium picolinate content mg/kg |
| The blank group | 0 | 0.782a | 18.01±1.11a | 0.00±0.00a |
| Low chromium feed group | 0.1 | 0.790a | 85.27±3.25b | 0.52±0.02b |
| High chromium feed group | 0.2 | 0.779a | 143.25±4.51c | 0.56±0.13b |
Result by the contrast test of table 1 can find out, while in feed, adding chromium picolinate 0.1wt%, the speed of growth of barley worm is not suppressed, and Organic Chromium content is apparently higher than control group; While adding chromium picolinate 0.2wt% in feed, the speed of growth of barley worm is not suppressed, and Organic Chromium content also is significantly increased, but concentration effect descends to some extent.As can be seen here, utilize the rich chromium feed of the embodiment of the present invention 1 preparation barley worm larva of feeding, do not affect the growth traits of barley worm larva, but can significantly improve the Organic Chromium content of barley worm larva, and wherein the Chromium Picolinate residual quantity is extremely low, therefore edible safety is high, with existing Organic Chromium replenishers in the market, compares with the obvious advantage.
According to the metabolism in animal body, studies show that, after chromium is absorbed and used, be all with amino acid, to become fit form to exist in animal body, therefore only needing to detect wherein have the chromium of how many original interpolation forms that do not transform get final product, and the chromium picolinate that then with total chromium, deducts interpolation is amino acid cooperation chromium.The additive added due to the present invention is the trivalent Organic Chromium, therefore after barley worm (insect) metabolism, does not have the possibility that changes inorganic chromium into.
The above embodiment is only the preferred embodiment for absolutely proving that the present invention enumerates, and protection scope of the present invention is not limited to this.Being equal to that those skilled in the art do on basis of the present invention substitutes or conversion, all within protection scope of the present invention.
Claims (10)
1. the preparation method of a rich Organic Chromium barley worm worm powder is characterized in that: comprise the following steps:
1) clean: after rich Organic Chromium barley larva is pressed to the long sizing screening of body, the washed with de-ionized water 2-5min through 80-95 ℃ by the worm alive of different brackets respectively, with the removal dirt and make barley worm larva not creep;
2) freeze drying: the thickness by the rich Organic Chromium barley worm larva after cleaning according to 1-5cm is laid on pallet, then freezing under put into-20-80 ℃, after freezing 1-2 hour, taking-up is put into freeze drier and is carried out lyophilization processing 20-40 hour, the water content of barley worm larva after drying and dehydrating is 3%-5%, and at the temperature of 0-5 ℃, stores after utilizing plastic sealing bag to seal up for safekeeping;
3) cold pressing:
3.1) preheat: the rich Organic Chromium barley worm larva stored is laid on the conveyer belt of microwave dryer and is preheated 2-7 minute in 50-80 ℃;
3.2) the hydraulic pressure squeezing: the rich Organic Chromium barley worm larva after preheating is packed into rapidly in the cavity of hydraulic oil press to pressurised extraction 2-15min;
4) ultramicro grinding: the de-oiling polypide that hydraulic pressure is squeezed in rear chamber is taken out standing, treat that temperature is down to normal temperature, first with pulverizer, be made into 5-10 purpose fine particle, the frequency modulation that to put it into afterwards the rolling wheel rotating speed again and be 350-480 rev/min, cyclonic wind unit frequency be 5-40HZ rolls in the Cyclonic micronizer and carries out ultra micro processing, to make 400-500 purpose ultra micro worm powder.
2. method according to claim 1, is characterized in that: step 1) described rich Organic Chromium barley larva is classified as two-stage 10-12cm and 12-15cm by body length.
3. method according to claim 1 is characterized in that: the rich Organic Chromium barley of described cultivation worm larva obtains by following concrete steps:
1), after the barley worm larva worm of cultivation reaches 60 ages in days age, the rich chromium feed that starts to feed, obtain described rich Organic Chromium barley worm larva after cultivating 4 weeks; Its concrete cultivating condition is as follows: the cultivation temperature is 25 ℃-27 ℃, and relative humidity is 65-75%, and whole culturing chamber is without direct sunlight, and the fluorescent lamp of 20-40W irradiates, and irradiation time is 8 hours/day; Cultivation density: larva cultivates in breeding box, and wherein the density of larva is 4-6 head/cm
2;
2) feedstuff feeding: adopt and take fine fodder and fed as main, cobalt blue pigment as the auxiliary mode of feeding, concentrated feed is thrown in weekly once, inventory is the heavy 20-40% of worm, wherein, the fine fodder of throwing in the 1st week is to be the above rich chromium feed and wheat bran of 1:1 by weight percentage, and the fine fodder of the 2nd week the-the 4th week is the above rich chromium feed; Cobalt blue pigment is thrown in once in every two days, and inventory is the heavy 10-30% of worm.
4. method according to claim 3; it is characterized in that: it is formulated as primary raw material that described rich chromium feed be take Chromium Picolinate, and described Chromium Picolinate is commercially available pyridine chromic formate, chromium malate, rutin chromium, chromium folate, stachyose chromium, taurine chromium or the Cr-enriched yeast powder of adjoining.
5. method according to claim 4, it is characterized in that: in described rich chromium feed, organic chromic content is 10mg/kg-200mg/kg.
6. method according to claim 4, it is characterized in that: described rich chromium feed also contains the raw material of following percentage by weight:
Wheat bran: 50-70%; Bacterium chaff: 0-30%; Dregs of beans: 0-10%; Corn protein powder: 0-3%; Soyabean protein powder: 0-3%; Moisture: 5-15%.
7. method according to claim 6 is characterized in that: described bacterium chaff is for once or the bacterium chaff of the rich chromium edible mushrooms such as rich chromium Pleurotus nebrodensis, rich chromium mushroom, rich chromium flat mushroom or Ganoderma rich in chromium that obtain after pulverizing of the rod of the bacterium after the secondary fruiting.
8. method according to claim 3, is characterized in that: the cauline leaf that described cobalt blue pigment is commercially available Chinese cabbage, potato or spinach; Described fine fodder and cobalt blue pigment ratio by weight percentage is 1:2.
9. a rich Organic Chromium barley worm worm powder, it is characterized in that: described rich Organic Chromium barley worm worm powder makes by the described method of claim 1-3.
10. rich Organic Chromium barley worm worm powder according to claim 9 is characterized in that: in described rich Organic Chromium barley worm worm powder, final organic chromic content is 10mg/kg-200mg/kg.
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