CN103477748B - Utilize the method for agricultural crop straw prevention and control soil-borne diseases of vegetable - Google Patents

Utilize the method for agricultural crop straw prevention and control soil-borne diseases of vegetable Download PDF

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CN103477748B
CN103477748B CN201310401977.XA CN201310401977A CN103477748B CN 103477748 B CN103477748 B CN 103477748B CN 201310401977 A CN201310401977 A CN 201310401977A CN 103477748 B CN103477748 B CN 103477748B
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waterflooding
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agricultural crop
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马艳
王光飞
顾志光
徐跃定
张建英
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Jiangsu Academy of Agricultural Sciences
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Abstract

The present invention relates to a kind of method utilizing agricultural crop straw prevention and control soil-borne diseases of vegetable, it is characterized in that: after agricultural crop straw being applied field, submerging treatment is carried out to soil, concrete steps are: soil temperature is more than 20 DEG C, residual for preceding crop in field body is removed clean, about 20 centimetres of high ribs are played in field surrounding fortifield village, the agricultural crop straw being crushed to 10-15 centimetre length is evenly spread fertilizer over the fields to field, then by dark for soil ploughing 18-20 centimetre, with irrigation water, submerging treatment is carried out 5 days to field, during waterflooding, the field depth of water remains on more than 5 centimetres, soil layer surface, dark by soil rotary tillage 18-20 centimetre after 5 days, continue to keep waterflooding 15 days, waterflooding is terminated when soil redox potential is reduced to 0-40mv, chemical fertilizer is applied after soil natural dries, again by crop of planting after soil rotary tillage, the amount of application of chemical fertilizer is: 30-40 kilogram of urea and 20-30 kg of phosphoric acid potassic fertilizer.

Description

Utilize the method for agricultural crop straw prevention and control soil-borne diseases of vegetable
Technical field
The present invention relates to a kind of method utilizing agricultural crop straw prevention and control soil-borne diseases of vegetable, evenly spread fertilizer over the fields to field after specifically agricultural crop straw suitably being pulverized, submerging treatment certain hour is carried out by after Soil tillage, then to drain water, to plant after soil natural dries vegetables, the effect of prevention and control soil-borne diseases of vegetable can be reached.Belong to and utilize agricultural wastes to carry out integrated control field to vegetable disease.
Background technology
China is large agricultural country, and agricultural crop straw output is large, distribution is wide, kind is many, is the precious resources of life of farmers and agricultural development for a long time always.In recent years, along with the fast development of crop breeding technology, high-yield crop kind is constantly updated and is added improving constantly of agricultural planting level, grain and yield of commercial crops rise year by year, the generation of agricultural crop straw (hereinafter referred to as " stalk ") also increases year by year, within 2010, national stalk theoretical resources amount is 8.4 hundred million tons, can collect stock number and be about 700,000,000 tons.Stalk kind based on paddy rice, wheat, corn etc., wherein, straw about 2.11 hundred million tons, wheat straw about 1.54 hundred million tons.Stalk main component is cellulose, hemicellulose and lignin, and natural degradation speed is slow in the environment, and the straw decomposition difficulty that degree of lignification is high is larger; In addition, still lack at present economical and technically all feasible, can the straw collecting of large-scale promotion and storaging and transport technology, make arbitrarily the abandoning of stalk, to burn phenomenon serious, not only cause the wasting of resources also to bring series of environmental problems.Therefore, accelerate to advance straw utilization, for stable Agro-ecology balance, alleviate resource constraint, alleviate environmental pressure all tool be of great significance.
2012, the facility cultivation area of China based on vegetables broke through 4,200,000 hm 2, than 139.5 ten thousand hm of 1999 last century Mos 2add nearly 3 times, growing vegetables has become in high-efficiency agriculture the leading industry driving farmer richness.But " four is high " that facility plantation is medium-term and long-term (high cropping index, high temperature, high humidity, high fertilizer) and continuous cropping make soil quality constantly worsen, soil salt accumulation, nutrient imbalance, microbiological paper method is unbalance, and pathogen grows in a large number, and soil-borne disease increases the weight of increasingly.In facility cultivation development area comparatively early, the annual economic loss caused by soil-borne diseases of vegetable accounts for the 20-30% of vegetables income from sales, soil-borne disease has not only become the bottleneck of engagement device Vegetable produce, and will be that facility cultivation industry faces and problem demanding prompt solution for a long time.
For the control of soil-borne disease, mainly contain the measures such as chemical pesticide, crop rotation, grafting, biopesticide, soil regulation and control, soilless culture and soil disinfection.Chemical pesticide has good effect to overground part disease control, but the proterties of soil complexity makes the control efficiency of chemical pesticide to soil-borne disease greatly reduce, strengthen pesticide dosage and cause again pathogen to develop immunity to drugs while bringing environmental pollution, agricultural product security risk; Crop rotation is a kind of approach of good solution soil-borne disease, but consider the regional characteristics of vegetable crop, the condition of sales of agricultural product and economic benefit, in the Vegetable produce base of scale, be difficult to the means adopting rice field-upland field rotation, and pattern effective, economic, technically feasible under also lacking scale condition at present; Grafting is mainly for soil-borne disease problem, but stock variety is limited, and wastes time and energy, and stock cost is also higher, and quality of agricultural product is significantly worse than the product of not grafting; Biopesticide has better effects to some soil-borne disease under controlled condition, but the soil colonization ability of biocontrol microorganisms is comparatively large by Effects of Factors such as environment, region, weathers, and preventive effect is unstable.The requirement of soilless culture to culture techniques such as cultivation matrix raw material and nutrient management is higher, and in conjunction with China's actual conditions, in the following long term, China remains and takes pattern as the leading factor with soil cultivation.With soil disinfection for the main soil control technique having reclamation result concurrently is the effective way of prevention and control soil-borne disease simultaneously, but the mechanism of action of different controlled material is different, effect is also variant, therefore targetedly select soil controlled material and method most important.
Domestic and international a lot of research and application shows that straw-returning can improve soil organic matter content, improves soil aggregate, increases the various nutrient content of soil, General Promotion soil Comprehensive Traits, culture fertility, improves plant recovery of nutrient, promote plant growth, improve output and improve quality of agricultural product.
Decomposition product species after straw-returning is a lot, and most of decomposition product can by crops as nutrient uptake and utilization.Wherein cause organic acid, phenolic acid class allelochemical that the mainly straw decomposition of large quantity research and concern produces, this two classes material promotes plant growth at low concentrations, certain murder by poisoning can be had to plant under high concentration, affecting plant to grow normally, is also that straw-returning process wants emphasis to consider and the problem that must be solved by technological means; But meanwhile, research and practice application a large amount of both at home and abroad also shows that these allelochemicals have strong growth inhibition even killing effect to many phytopathogens and weeds, may be used for the disease pest and weed of prevention and control various crop.In addition, the decomposition speed after straw-returning and the generation time of allelochemical and generation larger by the impact of stalk consumption and soil water content.Under dry farming, straw decomposition speed is slower than the decomposition speed under waterflooding state, and therefore, the time that allelochemical release continues is long, concentration is low.Our research shows every mu of also 400 kilograms, field wheat stalk, under waterflooding state, decomposition is planted after 14 days the vegetables such as capsicum, tomato, allelopathy effect is not had to plant, and under the conventional moisturizing state of soil, straw decomposition then there is obvious allelopathy effect to above-mentioned two kinds of vegetables after 14 days.
Waterflooding is the processing method of the sick pest such as worm, germ of a kind of ancient elimination soil.Also the report adopting waterflooding prevention and control soil-borne disease of crop is no lack of both at home and abroad.But much research also shows, independent waterflooding is difficult to reach the effect eliminating pathogen completely.
Having both at home and abroad manyly has the growth inhibition even bibliographical information of killing effect about straw decomposition product to multiple soil-borne pathogen, thinks that the allelochemical such as organic acid, phenolic acid that straw degradative produces will directly kill or suppress pathogen, other decomposition products of stalk can also provide abundant nutrition source for those to the insensitive edaphon of allelochemical (particularly bacterium) simultaneously, the Quantityanddiversity of remarkable increase edaphon, strengthen biological activity of soil, improve plant recovery of nutrient, promoting plant growth and enhance disease resistance can, but these reports are all under lab simulation dry farming, adopt improving straw mulching or stalk evenly to be admixed in soil after moisturizing cultivates certain hour, collect straw decomposition liquid in vitro, the growth inhibition effect to target pathogenic bacteria is measured under pure culture state, differ larger with the field soil environment of reality.And pathogen is directly inoculated in the soil containing stalk, under continuously submerging condition, so far there are no to the inhibitory action of pathogen in soil with to the open report of soil-borne diseases of vegetable control effect for on-spot study straw decomposition product.
Summary of the invention
The object of the invention is to: on the one hand, large for China's agricultural crop straw generation, lack efficient, economic, the workable production reality utilizing technology; On the other hand, high-efficiency agriculture fast development, modern plant soil-borne disease sharply occurs and increases the weight of year by year, still do not have at present economy, efficiently can the present situation of prevention and control measure of spread, a kind of method utilizing agricultural crop straw prevention and control soil-borne diseases of vegetable is proposed.
The object of the present invention is achieved like this: a kind of method utilizing agricultural crop straw prevention and control facilities vegetable soil-borne disease, it is characterized in that: after agricultural crop straw being applied field, submerging treatment is carried out to soil, concrete steps are: soil temperature is more than 20 DEG C, residual for preceding crop in field body is removed clean, about 20 centimetres of high ribs are played in field surrounding fortifield village, the agricultural crop straw being crushed to 10-15 centimetre length is evenly spread fertilizer over the fields to field, then by dark for soil ploughing 18-20 centimetre, with irrigation water, submerging treatment is carried out 5 days to field, during waterflooding, the field depth of water remains on more than 5 centimetres, soil layer surface, dark by soil rotary tillage 18-20 centimetre after 5 days, continue to keep waterflooding 15 days, waterflooding is terminated when soil redox potential is reduced to 0-40mv, chemical fertilizer is applied after soil natural dries, by crop of planting after soil rotary tillage, the amount of application of chemical fertilizer is: 30-40 kilogram of urea and 20-30 kg of phosphoric acid potassic fertilizer.
In the present invention, described agricultural crop straw refers to wheat, paddy rice or maize straw, and consumption is every mu of 400-600 kilogram, stalk moisture 12-14%.
The invention has the advantages that: first, stalk abundance and cheap and easy to get, stalk goes back to field and alleviates environmental pollution pressure as Term Fertilization, to improvement soil with extensively approved the benefit of plant growth; Secondly, waterflooding has obtained permanent checking and abundant affirmation to the improved effect of soil property, coordinate submerging treatment can accelerate the decomposition speed of stalk in soil after straw-returning, and make the material such as organic acid and phenolic acid concentrate a large amount of release at short notice, reach the effect killing or suppress pathogen.This invention input cost is low, and whole application is simple, is easy to operation, for the efficiency utilization of soil-borne disease control and stalk opens up new approach.
We gather multiple growing vegetables base, Jiangsu Province soil-borne disease and serious soil occurs carry out paddy rice stalk waterflooding 20 days process, result shows, compared with common waterflooding, in physicochemical property, stalk process soil organic matter content significantly improves, soil organic acid, total phenolics content significantly increase, and the electrical conductivity of soil, redox potential and ammonium nitrogen content then significantly decline.In biological property, in stalk submerging treatment, soil polyphenol oxidase is lived, cellulase activity is significantly higher than contrast, and stalk waterflooding extremely significantly increases number of bacteria.Carry out quantitative PCR detection result to pathogen Phytophthora capsici to show, conventional waterflooding also can reduce Phytophthora capsici quantity, but not remarkable, and stalk waterflooding then significantly reduces Phytophthora capsici quantity.Conventional waterflooding 20 days, the sick incidence of disease of capsicum is 50%, and waterflooding more than the 35 days incidences of disease are below 10%, and rice straw waterflooding 20 days incidences of disease are just 0.This technology not only shortens Submergence time but also significantly improves protection effect, is significant to facility operation character improvement, soil-borne disease prevention and control and the sustainable use of soil in protected field and the sustainable development of industrialized agriculture.
The present invention's cheaper starting materials used is easy to get, and method of operating is simple and easy, and input cost is low, and Be very effective is lasting.
Applicant is found by the research in early stage, submerging treatment is carried out again owing to apply quantity of organic material in soil after, during waterflooding, organic materials decomposition causes soil generation series of physical chemistry and biological respinse, finally causes the soil environment being unfavorable for that growth of pathogenic bacteria is bred.If every mu is used 600 kg of water rice straws, continuously submerging 20 days, reaches more than 95% to the elimination factor of P. capsici in soil, is 100% to the control effect of capsicum epidemic disease, and the elimination factor of common waterflooding to pathogen is only 60-65%, be about 65% to disease control effect; Use 400 kilograms of wheat stalks for every mu, the submerging treatment straw decomposition liquid of 14 days is 100% to the inhibiting rate of withered germ of water-melon spore germination, and common waterflooding is 20-30% to pathogen inhibition of germination.After straw-returning, waterflooding can also significantly increase soil organic matter content, bio-diversity and biologically active simultaneously, continues the generation suppressing soil-borne disease, has broad application prospects.
Accompanying drawing explanation
Fig. 1 is the impact of rice straw waterflooding on soil redox potential.
Fig. 2 is the impact of rice straw waterflooding on soil organic matter content.
Fig. 3 is the impact of rice straw waterflooding on soil organic acid content.
Fig. 4 is the impact of rice straw waterflooding on soil phenolic content.
Fig. 5 is the impact of rice straw waterflooding on the capsicum epidemic disease incidence of disease.
Fig. 6 is rice straw waterflooding in soil and the impact of capsicum rhizosphere Phytophthora capsici quantity.
Fig. 7 be wheat stalk decomposition liquid to the inhibitory action of withered germ of water-melon conidia germination, wherein: A figure is the effect of clear water contrast, B figure is the effect of adding wheat straw decomposition liquid.
The CK related in figure is blank group; F is conventional waterflooding group; FS is stalk waterflooding group.
Embodiment
embodiment 1
rice straw waterflooding is on the impact of soil physical and chemical property
Test soil is taken from Huaian Gao Gouzhen capsicum and is produced booth, and rice straw takes from academy of agricultural sciences of Jiangsu Province paddy field, pathogen be P. capsici ( phytophthoracapsici), can obtain from Chinese Academy of Agricultural Sciences's agricultural Culture Collection.Examination capsicum variety is supplied to be Lip river green pepper No. 5.Phytophthora capsici spore liquid is evenly added in test soil, make the mould spore concentration of soil epidemic disease be 200 epidemic disease mould spore/gram dry ground.3 process are established in test: (1) contrast (CK): keep soil water content at 16-18% after soil inoculation Phytophthora capsici, naturally place.(2) conventional waterflooding (flooding, F): carry out submerging treatment after soil inoculation Phytophthora capsici.(3) rice straw waterflooding (floodingwithstraw, FS): add 1%(W/W again after soil inoculation Phytophthora capsici) stalk, then carry out submerging treatment.Submerging treatment in conventional waterflooding and rice straw waterflooding is all carry out in Plastic Drum (end footpath × bore × height=25cm × 34cm × 30cm), and every barrel of fresh soil of 14kg, often processes repetition twice.During waterflooding, soil surface keeps 10cm water layer.Plastic Drum being placed in room temperature is under the environment of about 28 DEG C, 14 days submerging treatment time.After waterflooding terminates, drain surface water, then soil is spread out be placed in dark ventilation dry to water content be about 20%, measure soil physical and chemical property.
1.1 rice straw waterflooding are on the impact of redox potential
The results are shown in accompanying drawing 1.Rice straw waterflooding and the conventional submerging treatment Eh value of soil during waterflooding declines gradually, and rice straw waterflooding group fall is significantly greater than conventional waterflooding group.At the end of waterflooding, the Eh value of conventional waterflooding group and rice straw waterflooding group is respectively 112 and-116mv, and after adding stalk, waterflooding significantly reduces soil redox potential.Stalk is organic materials, decomposes and need consume oxygen, Soil Eh is significantly reduced under waterflooding situation, accelerates the death of pathogen or suppresses the growth and breeding of pathogen, reducing disease occurrence probability.
1.2 rice straw waterflooding are on the impact of soil organic matter content
The results are shown in accompanying drawing 2.During waterflooding, rice straw waterflooding group soil organic matter content rises to 2.32% gradually by 1.05%, and control group and conventional waterflooding group change less.Soil organic matter content is the important indicator of characteristics of soil fertility, and soil carries out rice straw submerging treatment and is conducive to increasing soil organic matter content, improves soil water-retaining, fertilizer conservation and cushioning effect.
1.3 rice straw waterflooding are on the impact of soil organic acid and phenolic content
From accompanying drawing 3 and accompanying drawing 4, during waterflooding control group and conventional waterflooding group organic acid and phenolic content change remarkable, and rice straw waterflooding group organic acid, phenolic content rise rapidly.At the end of waterflooding, the soil organic acid of rice straw waterflooding and phenolic content are 4.41 and 3.73 times of waterflooding group respectively, and existing document shows that these phenolic acid have strong growth inhibitory effect to Phytophthora capsici and other plant soil-borne pathogen.
the waterflooding of embodiment 2 rice straw is on the impact of activity of soil xylanase
Gather embodiment 1 submerging treatment dry after soil, measure activity of soil xylanase.
From table 1, the activity of soil xylanase of rice straw waterflooding is all higher than contrast and conventional waterflooding.Soil enzymatic activities is an important indicator of soil biology proterties, and generally, healthy soil has higher soil xylanase and lives.In addition, the main component of phytophthora blight of pepper cell wall is cellulose, and soil cellulase activity improves can suppress the growth of P. capsici or this germ of degrading, and reduces capsicum epidemic disease occurrence probability.
The waterflooding of table 1 rice straw is on the impact of activity of soil xylanase
Contrast Conventional waterflooding Rice straw waterflooding
Cellulase activity 0.14±0.01 0.16±0.02 0.31±0.02
Note: the unit that soil xylanase is lived is mg glucose/(10g72h).
embodiment 3: rice straw waterflooding is to the inhibitory action of Phytophthora capsici growth in soil
Soil embodiment 1 submerging treatment crossed adopts quantitative PCR (RT-PCR) method to measure Phytophthora capsici quantity in each process.Phytophthora capsici assay method: it is the FastDNASPINKitforSoil that MP company produces that soil DNA extracts used kit, extracts soil DNA according to operation instruction.Special primer is CAPFW(5'-TTTAGTTGGGGGTCTTGTACC-3') and CAPRV1(5'-CCTCCACAACCAGCAACA-3'), object clip size is about 450bp.RT-PCR reaction kit adopts the SYBRpremixExTaq of TaKaRa company.Fluorescent quantitative PCR system is: 2 × SYBRPreMixExTaq (TaKaRa) 10 μ L, 50 × ROXReferenceDye II (TaKaRa) 0.4 μ L, 20 μm of each 0.4 μ L of ol/L primer, template 2 μ L, ddH2O6.8 μ L, amplification system is 20 μ L.Response procedures is: 95 DEG C, 30s, 1 circulation; 95 DEG C of 5s, 60 DEG C of 34s, 40 circulations.Quantitative fluorescent PCR instrument is 7500 types (AppliedBiosystems, USA).Will containing 10,40,160,6 standard soil samples of 640,2560,10240 Phytophthora capsici spore/gram dry ground and the soil DNA of Phytophthora capsici concentration samples to be measured carry out SYBRPCR amplification simultaneously, draw out calibration curve with 7500software, and calculate the concentration of unknown sample soil Phytophthora capsici.
Bright as can be seen from Table 2, before waterflooding starts, in soil, the initial concentration of Phytophthora capsici spore is 218.1 Phytophthora capsicis/gram dry ground; After waterflooding terminates, the Phytophthora capsici quantity of contrast, conventional waterflooding and rice straw waterflooding is 31.5,6.8 and 2.5 Phytophthora capsicis/gram dry ground respectively, and rice straw waterflooding significantly reduces the quantity of Phytophthora capsici in soil.
The waterflooding of table 2 rice straw is on the impact of Phytophthora capsici quantity
Contrast Conventional waterflooding Rice straw waterflooding
Phytophthora capsici quantity (individual spore/gram soil) 31.5 6.8 2.5
the waterflooding of embodiment 4 rice straw is to the control effect of capsicum epidemic disease and the lasting inhibitory action to Phytophthora capsici
The soil of submerging treatment in embodiment 1 is dispensed in plastic basin alms bowl, transplant 4 the leaf phase capsicum, 45 days " Invest, Then Investigate " capsicum incidences of disease.
From accompanying drawing 5: the incidence of disease of contrast, conventional waterflooding and rice straw waterflooding is respectively 100%, 16.67% and 0, illustrate conventional waterflooding can in certain degree prevention and control capsicum epidemic disease, and rice straw waterflooding can suppress the generation of capsicum epidemic disease completely.Visible, rice straw waterflooding has higher control effect to capsicum epidemic disease.
Accompanying drawing 6 shows, after capsicum of planting, control group Phytophthora capsici quantity sharply rises, and conventional waterflooding group has certain rising.Capsicum plant after the 45th day time, contrast and conventional waterflooding two groups of soil Phytophthora capsici concentration be respectively 339.2 and 35.9 Phytophthora capsicis/gram dry ground, rice straw waterflooding group does not then detect Phytophthora capsici.Visible rice straw submerging treatment not only significantly can reduce the quantity of Phytophthora capsici in soil during waterflooding, and still can continue to suppress Phytophthora capsici at the growth and breeding of capsicum rhizosphere after capsicum is planted, and reaches the effect of lasting prevention and control disease.
the decomposition liquid of embodiment 5 wheat straw waterflooding is to the growth inhibition effect of withered germ of water-melon
By wheat stalk chopping to 5-10 centimetre, with 0.5%(W/W) consumption evenly join in the soil of natural air drying, soil is with embodiment 1, soil is dispensed in Plastic Drum, 3 kilograms, every barreled soil, add after running water makes soil fully saturated, upper soll layer keeps the water layer of 10 centimetres, left at room temperature waterflooding 14 days, contrasts the conventional waterflooding of soil for not adding wheat stalk.Topsoil water is outwelled after 14 days, by the centrifugal 15min of soil 6000r/min of waterflooding, obtain supernatant, supernatant is obtained aseptic decomposition liquid by the sterilised membrane filter of 0.22 μm, adopt sessile drop method to measure decomposition liquid to the impact of withered germ of water-melon conidia germination.
In fig. 7, A figure represents withered germ of water-melon spore germination situation when not adding wheat straw decomposition liquid (i.e. clear water contrast), and B figure is withered germ of water-melon spore germination situation after interpolation wheat straw decomposition liquid.Microscopy results shows: after 8 hours, and do not add the clear water contrast of decomposition liquid, withered germ of water-melon germination rate is more than 95%, and is only 15-20% through the conidial germination rate of withered germ of water-melon of wheat stalk decomposition liquid process.Namely A figure spore germination rate is high, and B figure does not almost have spore germination, and visible, wheat stalk decomposition liquid has stronger inhibitory action to withered germ of water-melon spore germination.

Claims (2)

1. one kind utilizes the method for agricultural crop straw prevention and control soil-borne diseases of vegetable, it is characterized in that: after agricultural crop straw being applied field, submerging treatment is carried out to soil, concrete steps are: soil temperature is more than 20 DEG C, residual for preceding crop in field body is removed clean, field surrounding is played the rib of 20 centimetres high with fortifield village, the agricultural crop straw being crushed to 10-15 centimetre length is evenly spread fertilizer over the fields to field, then by dark for soil ploughing 18-20 centimetre, with irrigation water, submerging treatment is carried out 5 days to field, during waterflooding, the field depth of water remains on more than 5 centimetres, soil layer surface, dark by soil rotary tillage 18-20 centimetre after 5 days, continue to keep waterflooding 15 days, waterflooding is terminated when soil redox potential is reduced to 0-40mv, chemical fertilizer is applied after soil natural dries, again by crop of planting after soil rotary tillage, the amount of application of chemical fertilizer is: 30-40 kilogram of urea and 20-30 kg of phosphoric acid potassic fertilizer.
2. the method utilizing agricultural crop straw prevention and control soil-borne diseases of vegetable according to claim 1, is characterized in that: described agricultural crop straw refers to wheat, paddy rice or maize straw, consumption is every mu of 400-600 kilogram, stalk moisture 12-14%.
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