CN103463668B - A kind of preparation method of silk fibroin-calcium alginate biological wound porous dressing - Google Patents
A kind of preparation method of silk fibroin-calcium alginate biological wound porous dressing Download PDFInfo
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- CN103463668B CN103463668B CN201310401268.1A CN201310401268A CN103463668B CN 103463668 B CN103463668 B CN 103463668B CN 201310401268 A CN201310401268 A CN 201310401268A CN 103463668 B CN103463668 B CN 103463668B
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- 239000000648 calcium alginate Substances 0.000 title claims abstract description 23
- 229960002681 calcium alginate Drugs 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 108010022355 Fibroins Proteins 0.000 claims abstract description 34
- 235000010410 calcium alginate Nutrition 0.000 claims abstract description 15
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241000255789 Bombyx mori Species 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 108010025899 gelatin film Proteins 0.000 claims abstract description 6
- 238000004108 freeze drying Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 4
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 230000000844 anti-bacterial effect Effects 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 150000001298 alcohols Chemical class 0.000 claims description 2
- 230000003078 antioxidant effect Effects 0.000 claims description 2
- 238000007796 conventional method Methods 0.000 claims description 2
- 239000003755 preservative agent Substances 0.000 claims description 2
- 230000002335 preservative effect Effects 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims 1
- 229910001628 calcium chloride Inorganic materials 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 13
- 239000012460 protein solution Substances 0.000 abstract description 6
- 239000008367 deionised water Substances 0.000 abstract description 3
- 229910021641 deionized water Inorganic materials 0.000 abstract description 3
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 235000013402 health food Nutrition 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 206010052428 Wound Diseases 0.000 description 20
- 208000027418 Wounds and injury Diseases 0.000 description 20
- 210000003491 skin Anatomy 0.000 description 14
- 239000012528 membrane Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 206010072170 Skin wound Diseases 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 206010042496 Sunburn Diseases 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000036783 anaphylactic response Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
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- 230000003115 biocidal effect Effects 0.000 description 1
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- 230000001010 compromised effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
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- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229930014550 juvenile hormone Natural products 0.000 description 1
- 239000002949 juvenile hormone Substances 0.000 description 1
- 150000003633 juvenile hormone derivatives Chemical class 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
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- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
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Abstract
The present invention relates to skin care item and technical field of medical instruments is the preparation method of a kind of silk fibroin-calcium alginate biological wound dressing. The concrete preparation process adopted is dissolved by the fibrous fibroin formed after degumming silkworm cocoons, the silk fibroin water solution of Different Weight concentration is obtained after filtering, dialyse, concentrating, deionized water, calcium alginate are mixed with a certain amount of silk fibroin protein solution, the gelatinous solution obtained, gelatinous solution is obtained gel film under room temperature, by gel film lyophilization, namely obtain wound surface porous gel dressing. The present invention utilizes excellent biocompatibility; The fibroin albumen and the calcium alginate that use in raw material are all natural active matter, it is common to for health food industry, do not produce to stimulate to skin, good hygroscopicity, and water absorption is splendid; Fibroin and calcium alginate all have high water retention performance, thus skin has good affine performance.
Description
Technical field
The present invention relates to skin care item and technical field of medical instruments, specifically, be the preparation method of a kind of silk fibroin-calcium alginate biological wound dressing.
Background technology
Skin is the organ that human body is maximum, is the first physical barriers between human body and external environment, has the extraneous harmful substance of prevention and invades, drains and prevent moisture evaporation, regulate body temperature, make body be in the important function such as a stable environment. By using different Wound dressings can effectively facilitate reparation and the accelerating wound healing of injured skin. Traditional Wound dressing is not good due to hydrophilicity so that Wound dressing is harder, more crisp, often has secondary physical to injure compromised skin, and antibacterial can enter the marginal growth of dressing; Secondly, traditional Wound dressing microstructure aperture is big, porosity is low, and skin breathability is bad, and sustained drug release effect is not good so that promote that skin healing effect neither be fine.
Fibroin albumen contains 18 seed amino acids to the great nutritive value of human body, By Juvenile Hormone is because of the mechanical property of its uniqueness and good biocompatibility, antibiotic property, easy without anaphylaxis, degradability and stability Purification, and essence is the construction features of protein, regenerated silk fibroin fiber, silk fibroin powder, fibroin protein film, fibroin porous material can be obtained after treatment, be applied to the fields such as food, cosmetics, medicine, biomaterial.Regenerated Silk Fibroin Film, the adhesiveness of cell is relatively strong, and epidermis cell and fibroblast can grow well on fibroin membrane, and fibroin albumen biomembrane uses for wound dressing, wound dressing of burn after operation, contributes to wound healing and non-stimulated. Calcium alginate application on skin care item is based on the characteristic of its maintenance cell viability with excellence and biomacromolecule activity; protecting film one layer special can be formed at cell surface layer; keep the original nutrition of skin and moisture, it is to avoid skin sunburn and melanin deposition. The mucus precipitated out from film not only moistens Skin Cell, also has the function being radiate by external heat. Thus protecting the skin will not be impaired.
Summary of the invention
The present invention is directed to the deficiency existed in conventional fabrication techniques, improved, it is provided that a kind of technique is simple, processes convenient novel preparation for processing. The present invention, with natural macromolecular material fibroin albumen for carrier, adds the biological wound-surface gel dressing that calcium alginate is made. Wound dressing prepared by the present invention is mainly used in skin wound medical dressing, and ventilative and hygroscopicity can be excellent, is a kind of desirably skin wound dressing and skin care item. The concrete technical scheme of the present invention is as follows:
The preparation method that the invention discloses a kind of silk fibroin-calcium alginate biological wound porous dressing, the concrete preparation process adopted is as follows:
(1) the fibrous fibroin formed after degumming silkworm cocoons is dissolved, after filtering, dialyse, concentrating, obtain the silk fibroin water solution of Different Weight concentration;
(2) deionized water, calcium alginate are mixed with a certain amount of silk fibroin protein solution, by conventional method heating for dissolving, stir so that be cross-linked with each other between calcium alginate and fibroin albumen, the gelatinous solution obtained;
(3) gelatinous solution obtained in step (2) is obtained gel film under room temperature;
(4) by gel film lyophilization in step (3), wound surface porous gel dressing is namely obtained.
As further improvement, the wound surface porous gel dressing in step of the present invention (4) can process through alcohols so that it is mechanical property strengthens.
As further improvement, Wound dressing of the present invention can add one or more in the appropriate antioxidant activity factor, preservative, antibacterial according to actual needs.
Compared with prior art, the present invention has feature highlighted below:
(1) excellent biocompatibility; The fibroin albumen and the calcium alginate that use in raw material are all natural active matter, have been commonly used to health food industry, do not produce to stimulate to skin.
(2) permeability is excellent; Owing to silk fibroin-calcium alginate biological wound dressing containing three-dimensional porous structure, good permeability, the release of multiple somatomedin after adding medicine, can be promoted, promote that the regeneration of trauma skin, repair and reconstruction make healing speed accelerate.
(3) good hygroscopicity, water absorption is splendid; Fibroin and calcium alginate all have high water retention performance, thus skin has good affine performance.
(4) present invention overcomes existing Wound dressing Problems existing, it is provided that a kind of technique is simple, is suitable for industrialized production.
(5) with low cost, environment friendly and pollution-free.
Detailed description of the invention
Below by specific embodiment, technical scheme being described in further detail, following example are explanation of the invention and the invention is not limited in following example.
Embodiment 1
(1) the fibrous fibroin formed after degumming silkworm cocoons is dissolved in concentration in LiBr solution, through filtration, dialysis, concentration after obtain 3%(w/w) silk fibroin protein solution;
(2) 80-120mg calcium alginate is mixed with 8-12mL silk fibroin protein solution, 40-60 DEG C of heating for dissolving stirring of water-bath so that be cross-linked with each other between calcium alginate and fibroin albumen, the gelatinous solution obtained;
(3) gelatinous solution obtained in (2) is fallen in molding vessel, make gel solidification, obtain dressing membrane;
(4) dressing membrane (3) obtained, dries through freezer dryer after-20 DEG C to-60 DEG C freezings, namely obtains wound surface gel dressing. Embodiment 2
(1) the fibrous fibroin formed after degumming silkworm cocoons is dissolved in concentration C aCl2In solution, through filtration, dialysis, concentration after obtain 5%(w/w) silk fibroin protein solution;
(2) 80-120mg calcium alginate is mixed with 8-12mL silk fibroin protein solution, 40-60 DEG C of heating for dissolving stirring of water-bath so that be cross-linked with each other between calcium alginate and fibroin albumen, the gelatinous solution obtained;
(3) gelatinous solution obtained in (2) is fallen in molding vessel, make gel solidification, obtain dressing membrane;
(4) dressing membrane (3) obtained, dries through freezer dryer after-20 DEG C to-60 DEG C freezings, namely obtains wound surface gel dressing;
(5) Wound dressing in (4) is immersed 75%(v/v) 12h in alcoholic solution, fixing established gel, replace with deionized water afterwards, remove the ethanol of residual, after natural drying, namely obtain wound surface gel dressing.
Finally, in addition it is also necessary to it is noted that listed above is only specific embodiments of the invention. It is clear that the invention is not restricted to above example is sub, it is also possible to there are many deformation. All deformation that those of ordinary skill in the art can directly derive from present disclosure or associate, are all considered as protection scope of the present invention.
Claims (2)
1. the preparation method of a silk fibroin-calcium alginate biological wound porous dressing, it is characterised in that the concrete preparation process adopted is as follows:
(1) the fibrous fibroin formed after degumming silkworm cocoons being dissolved in LiBr solution, obtaining mass concentration after filtering, dialyse, concentrating is the silk fibroin water solution of 3%; Or the fibrous fibroin formed after degumming silkworm cocoons is dissolved in CaCl2Solution, obtaining mass concentration after filtering, dialyse, concentrating is the silk fibroin water solution of 5%;
(2) 80-120mg calcium alginate is added directly in the silk fibroin water solution of 8-12mL and mixes, by conventional method heating for dissolving, water-bath 40-60 DEG C, stir so that be cross-linked with each other between calcium alginate and fibroin albumen, the gelatinous solution obtained;
(3) fall to obtain gel film at normal temperatures in molding vessel by the gelatinous solution obtained in step (2);
(4) by gel film in step (3) through-20 DEG C to-60 DEG C lyophilizations, namely obtaining wound surface porous gel dressing, described wound surface porous gel dressing processes through alcohols so that it is mechanical property strengthens.
2. the preparation method of silk fibroin-calcium alginate biological wound porous dressing according to claim 1, it is characterised in that described Wound dressing adds one or more in the appropriate antioxidant activity factor, preservative, antibacterial.
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| CN201310401268.1A CN103463668B (en) | 2013-09-05 | 2013-09-05 | A kind of preparation method of silk fibroin-calcium alginate biological wound porous dressing |
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| CN201310401268.1A CN103463668B (en) | 2013-09-05 | 2013-09-05 | A kind of preparation method of silk fibroin-calcium alginate biological wound porous dressing |
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| CN103463668B true CN103463668B (en) | 2016-06-15 |
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Families Citing this family (17)
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| CN103806123B (en) * | 2014-03-03 | 2016-01-06 | 武汉理工大学 | Fibroin albumen/sodium alginate composite fiber and preparation method thereof |
| CN104327283B (en) * | 2014-10-16 | 2017-01-11 | 苏州经贸职业技术学院 | Composite silk fibroin hydrogel and preparation method and application thereof |
| CN104857569A (en) * | 2015-05-06 | 2015-08-26 | 浙江大学 | Preparation method of fibroin and graphene oxide composite bracket material |
| CN105056300B (en) * | 2015-09-02 | 2018-06-05 | 无锡市第三人民医院 | A kind of preparation method of the biomimetic porous stent of dermal tissue |
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| CN106279749A (en) * | 2016-09-22 | 2017-01-04 | 大连工业大学 | A kind of alginate and the preparation method of fibroin albumen composite sponge |
| CN106333939A (en) * | 2016-10-09 | 2017-01-18 | 常州亚环环保科技有限公司 | Method for preparing medicinal transdermal material |
| CN107519535B (en) * | 2017-09-28 | 2020-05-05 | 武汉理工大学 | Degradable sodium alginate/fibroin double-layer skin tissue engineering scaffold material and preparation method thereof |
| CN107823694B (en) * | 2017-11-08 | 2020-11-27 | 西南大学 | Moisture-retaining double-layer sponge dressing and preparation method thereof |
| CN107998449A (en) * | 2017-12-15 | 2018-05-08 | 杭州捷诺飞生物科技股份有限公司 | A kind of 3D printing high intensity bio-ink material |
| CN108969790B (en) * | 2018-07-08 | 2021-06-22 | 山东复本生物科技有限公司 | Absorbable porous medical dressing with scar regeneration resisting function and preparation method thereof |
| CN109157672B (en) * | 2018-08-16 | 2020-06-23 | 浙江大学 | Preparation method of fibroin-oligopeptide blood coagulation microspheres |
| CN109512577B (en) * | 2018-10-08 | 2022-02-18 | 武汉纺织大学 | Healing-promoting biological environment-friendly medical dressing and manufacturing method thereof |
| CN109260507A (en) * | 2018-10-31 | 2019-01-25 | 广东泰宝医疗科技股份有限公司 | A kind of high liquid-absorbing fibroin albumen haemostatic membrane and preparation method thereof |
| CN112089883B (en) * | 2020-09-14 | 2022-01-04 | 海南希睿达生物技术有限公司 | Medical cold compress dressing and preparation method thereof |
| CN114081991B (en) * | 2021-11-12 | 2023-01-10 | 重庆医科大学 | Composite transparent hydrocolloid dressing with bioactivity based on fibroin/alginate fibers and preparation method thereof |
| CN114832158A (en) * | 2022-05-10 | 2022-08-02 | 武汉理工大学 | Preparation method of composite mesenchymal stem cell-based skin injury repair scaffold material |
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