CN109260507A - A kind of high liquid-absorbing fibroin albumen haemostatic membrane and preparation method thereof - Google Patents
A kind of high liquid-absorbing fibroin albumen haemostatic membrane and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/0047—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/02—Surgical adhesives or cements; Adhesives for colostomy devices containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/06—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/08—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/108—Specific proteins or polypeptides not covered by groups A61L24/102 - A61L24/106
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
- D01F8/16—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one other macromolecular compound obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds as constituent
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/18—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from other substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/236—Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Abstract
The invention discloses a kind of high liquid-absorbing fibroin albumen haemostatic membrane and preparation method thereof, the high liquid-absorbing energy fibroin albumen haemostatic membrane is composed of the following components according to parts by weight: 5-25 parts of polyethylene glycol oxide, 10-50 parts of fibroin albumen, 5-15 parts of sodium alginate, 0.5-1 parts of nano silica.The preparation method is realized by following steps: (1) preparation of haemostatic membrane spinning material, the spinning of (2) haemostatic membrane, (3) haemostatic membrane crosslinking Treatment.The present invention is resulting to have that high liquid-absorbing fibroin albumen haemostatic membrane haemostatic effect is good, can absorb a large amount of wound exudates, with good gas permeability and biocompatibility.
Description
Technical field
The invention belongs to hemostatic material preparation technical field more particularly to a kind of high liquid-absorbing fibroin albumen haemostatic membrane and its
Preparation method.
Background technique
It is injured unavoidable in daily life, and stopping blooding is then an important step of emergency aid and treatment, especially daily life
The operative treatment etc. of the sudden trauma, hospital that occur in work, quick-acting haemostatic powder are all indispensable a part.And effectively stop
Blood agent is usually all expensive, and drug effect is lower or causes various safety problems.Therefore a kind of efficient, cheap, nontoxic pair is found
The novel hemostatic material of effect seems most important.The haemostatic measures taken in the past mainly tie wound using tourniquet or bandage
Mouthful, then use gauze pressure dressing.These Hemostasis have played important function for rescuing the wounded in a short time.But due to
Tourniquet can not give treatment to big angiorrbagia, insecure and easy to fall off secondary bleeding etc. be caused to make it quickly in wound surface adherency
It is replaced by novel hemostatic material.With the development and progress of modern medical techniques, hemostatic material and medical instrument rapidly and efficiently
It comes into being.They have certain effect in terms of hemostasis, but in view of these hemostatic material higher costs, the secondary sense of initiation
Dye, it is not degradable in vivo the disadvantages of and be unable to wide popularization and application.
Fibroin albumen is once widely used in textile industry as a kind of natural fiber material.Recent studies indicate that
Fibroin albumen not only has a variety of amino acid necessary to human body, non-toxic to body, without sensitization and stimulation, and can portion
Decomposing biological degradation.In addition, there is fibroin albumen good gas permeability and performance of keeping humidity to promote the quick healing of wound simultaneously；And
The porous film material being fabricated to also has certain flexibility and tensile property, is expected to become a kind of good hemostatic material.
Summary of the invention
It is an object of the invention to insufficient for existing hemostatic material absorbent, the deficiencies of bleeding stopping period is longer, mention
For a kind of high liquid-absorbing fibroin albumen haemostatic membrane and preparation method thereof, the high liquid-absorbing fibroin albumen haemostatic membrane immunogenicity is low,
Material source is abundant, has good biocompatibility and degradability.And matrix used by this kind of haemostatic membrane is biology
Degradation material can be degraded into harmless small molecule and exclude in vitro with human circulation.
To achieve the above object, the technical scheme is that a kind of high liquid-absorbing fibroin albumen haemostatic membrane and, it is described
For high liquid-absorbing fibroin albumen haemostatic membrane by polyethylene glycol oxide, fibroin albumen, sodium alginate and nano silica composition are further
, the high liquid-absorbing fibroin albumen haemostatic membrane is according to parts by weight by 5-25 parts of polyethylene glycol oxide, fibroin albumen 10-50
Part, 5-15 parts of sodium alginate, 0.5-1 parts of nano silica compositions.
Preferably, the polyethylene glycol oxide is 1 × 106It is purchased from Sigma-Aldrich, the alginic acid
The viscosity of sodium is 200Pas, is purchased from Qingdao Ming Yue Co., Ltd, and the nano silica is it is characterized in that, its partial size point
Cloth is 150-250 nm.
Further, the fibroin albumen extracts gained from silk, and specific extracting method is as follows: weighing certain mass
Silk raw silk, weighed according to standard proportional (silk quality/g: distilled water volume/L: natrium carbonicum calcinatum quality/g=10:1:5)
Distilled water and natrium carbonicum calcinatum.Distilled water is first added, adds natrium carbonicum calcinatum, 100 DEG C of heating, until the inside liquid boils completely
Silk raw silk is added after rising, heats 30 minutes.After 30min, silk is taken out wash with distilled water, until the clarification of water washed out
It is transparent.It is reheated 30 minutes according to above step.After experiment, obtained fibroin albumen is placed in 60 DEG C of baking ovens and is dried
It is spare；
To achieve the above object, a kind of another technical solution of the invention are as follows: preparation side of high liquid-absorbing fibroin albumen haemostatic membrane
Method, the preparation method are realized by following steps: (1) preparation of haemostatic membrane spinning material, the spinning of (2) haemostatic membrane, (3)
Haemostatic membrane crosslinking Treatment；
Further, the preparation specific steps of the high liquid-absorbing fibroin albumen haemostatic membrane are as follows:
(1) preparation of haemostatic membrane spinning material:
The preparation of S1 fibroin albumen/nano silica spinning solution: a certain amount of fibroin albumen is weighed by recipe ratio and is dissolved
In trifluoroethanol, be configured to mass concentration be 5-10% solution, preferably 10%, then by recipe ratio be added nanometer titanium dioxide
Silicon stirs 2-3h, preferably 2.5h under room temperature, spare；
The preparation of S2 polyethylene glycol oxide/sodium alginate spinning solution: it weighs a certain amount of polyethylene glycol oxide and is dissolved in distilled water, match
The polyethylene oxide solutions that mass concentration is 5-10%wt, the preferred 10%wt of concentration is made；Weigh a certain amount of sodium alginate dissolution
In distilled water, it is configured to the sodium alginate soln that mass concentration is 2-3%, the preferred 3%wt of concentration；Facilitate by matching by polyoxyethylene
Alkene solution and sodium alginate soln mixing, stir evenly, spare after defoaming；
(2) spinning of haemostatic membrane:
S1 is by formula respectively by fibroin albumen/nano silica spinning solution (A liquid) and polyethylene glycol oxide/sodium alginate spinning solution
(B liquid) is added in the corresponding syringe of electrostatic spinning machine, is stood 30-60 min, is drained the air of syringe；
It is 10-20 cm, preferably 15cm that S2, which adjusts the distance between spinning machine receiver and spinning nozzle,；High voltage power supply is connected, is adjusted
To 10 ~ 20 kV, preferably 15kV；Start syringe pump, the output speed for being loaded with the syringe pump of A liquid syringe is adjusted to 0.5-1.5
ML/h, preferably 1 mL/h；The output speed for being loaded with the syringe pump of B liquid syringe is adjusted to 0.6-1.0 mL/h, preferably 0.8 mL/
h；Start receiver and receive spinning, the revolving speed of receiver is set as 200 ~ 350 rpm, preferably 250rpm；To wherein one group of injection
After spinning solution in device exports completely, high voltage power supply is closed, and remove lower spinning film；
(3) haemostatic membrane crosslinking Treatment:
It weighs a certain amount of anhydrous calcium chloride to be dissolved in dehydrated alcohol, is configured to the calcium chloride that mass concentration is 2-5%wt and is crosslinked
Then obtained spinning film is soaked in crosslinked fluid 12-24h, then washs 2-3 with dehydrated alcohol by liquid, the preferred 5%wt of concentration
Time, it is placed in 60 DEG C of vacuum drying ovens and dries to get a kind of high liquid-absorbing fibroin albumen haemostatic membrane；
Preferably, the quantity of the syringe is 8-16, and by the difference of the spinning solution filled in syringe, is divided into A group
With B group, accordingly, A group syringe is controlled by A syringe pump, and B group syringe is controlled by B syringe pump；The connection of A group syringe
Spinning nozzle corresponds to A group spinning nozzle, and the spinning nozzle of the connection of B group syringe corresponds to B group spinning nozzle, A group spinning
Spray head and B group spinning nozzle are alternately fixed on side by side in order on the fixed slide unit of spinning nozzle；
Further, the spinning nozzle, which is characterized in that the spinning injector head aperture is 0.5-0.8mm, excellent
Select 0.6mm.
Beneficial effects of the present invention:
(1) present invention prepares a kind of haemostatic membrane of tool porous network structure by alternately electrostatic spinning technique, obtained
Its fibre diameter of haemostatic membrane is nanoscale, can effectively provide the absorbent of haemostatic membrane, and the good three dimensional network of this kind of haemostatic membrane
Shape structure is conducive to the aggregation of blood platelet, can play the effect of hemostasis in a short time.
(2) for the present invention with fibroin albumen, sodium alginate, nano silica and polyethylene glycol oxide are to stop prepared by raw material
Blood film has multiple hemostatic mechanism, and nano silica has the hydrone in selective absorbing blood, without absorbing blood
Middle other compositions, lead to the concentration of blood platelet and coagulation factor, to achieve the purpose that hemostasis；Sodium alginate can be that wound connects
Gel is formed when touching to achieve the effect that hemostasis；Fibroin albumen, which can convert fibrinogen into, can promote wound circumference blood
The fibrin of solidification reaches hemostasis purpose by sealing blood vessels.
Detailed description of the invention
Fig. 1 is the comparison diagram of the imbibition specific gravity of Examples 1 to 3 and comparative example；
Fig. 2, which is that its corresponding cell is opposite after Examples 1 to 3 and comparative example co-culture 1 day and 7 days with vascular endothelial cell, to be proliferated
The comparative result figure of rate；
Fig. 3 is the Activated partial thromboplastin time test result comparison diagram of Examples 1 to 3 and comparative example；
Fig. 4 is the blood coagulation protoenzyme time test comparative result figure of Examples 1 to 3 and comparative example.
Specific embodiment
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
A kind of high liquid-absorbing fibroin albumen haemostatic membrane is prepared in the present invention as follows:
(1) preparation of haemostatic membrane spinning material:
The preparation of S1 fibroin albumen/nano silica spinning solution: a certain amount of fibroin albumen is weighed by recipe ratio and is dissolved
In trifluoroethanol, it is configured to the solution that mass concentration is 10%, nano silica then is added by recipe ratio, is stirred under room temperature
2.5h is mixed, it is spare；
The preparation of S2 polyethylene glycol oxide/sodium alginate spinning solution: it weighs a certain amount of polyethylene glycol oxide and is dissolved in distilled water, match
The polyethylene oxide solutions that mass concentration is 10%wt are made；It weighs a certain amount of sodium alginate to be dissolved in distilled water, be configured to
The sodium alginate soln that mass concentration is 3%；By with facilitating polyethylene oxide solutions and sodium alginate soln mixing, stirring is equal
It is even, it is spare after defoaming；
(2) spinning of haemostatic membrane:
S1 is by formula respectively by fibroin albumen/nano silica spinning solution (A liquid) and polyethylene glycol oxide/sodium alginate spinning solution
(B liquid) is added in the corresponding syringe of electrostatic spinning machine, is stood 60 min, is drained the air of syringe；
It is 15cm that S2, which adjusts the distance between spinning machine receiver and spinning nozzle,；High voltage power supply is connected, 15kV is adjusted to；Starting note
Pump is penetrated, the output speed for being loaded with the syringe pump of A liquid syringe is adjusted to 1 mL/h；It is loaded with the output of the syringe pump of B liquid syringe
Speed is adjusted to 0.8 mL/h；Start receiver and receive spinning, the revolving speed of receiver is set as 250rpm；To wherein one group of injection
After spinning solution in device exports completely, high voltage power supply is closed, and remove lower spinning film；
(3) haemostatic membrane crosslinking Treatment:
It weighs a certain amount of anhydrous calcium chloride to be dissolved in dehydrated alcohol, is configured to the calcium chloride that mass concentration is 5%wt and is crosslinked
Then obtained spinning film is soaked in crosslinked fluid for 24 hours, is then washed 2-3 times with dehydrated alcohol by liquid, be placed in 60 DEG C of vacuum and dry
Drying is in case to get a kind of high liquid-absorbing fibroin albumen haemostatic membrane；
Embodiment 1
The present invention prepares a kind of high liquid-absorbing fibroin albumen haemostatic membrane according to the above method, wherein the dosage of polyethylene glycol oxide be 5 parts,
The dosage of fibroin albumen is 50 parts, the dosage of sodium alginate is 15 parts, the dosage of nano silica is 1 part of composition.
Embodiment 2
The present invention prepares a kind of high liquid-absorbing fibroin albumen haemostatic membrane according to the above method, and wherein the dosage of polyethylene glycol oxide is 25
Part, the dosage of fibroin albumen be 10 parts, the dosage of sodium alginate is 5 parts, the dosage of nano silica is 0.5 part of composition.
Embodiment 3
The present invention prepares a kind of high liquid-absorbing fibroin albumen haemostatic membrane according to the above method, and wherein the dosage of polyethylene glycol oxide is 15
Part, the dosage of fibroin albumen be 30 parts, the dosage of sodium alginate is 10 parts, the dosage of nano silica is 0.75 part of composition.
Embodiment 4
Comparative example: (obtained by a kind of biological absorbable haemostatic membrane of application reference number 201410150503.7)
Experimental group 1~3: for a kind of resulting high liquid-absorbing fibroin albumen haemostatic membrane of Examples 1 to 3.
(1) detection of absorbent
A kind of high liquid-absorbing fibroin albumen haemostatic membrane prepared by above-described embodiment 1~3 is carried out absorbent with comparative example to comment
Valence experiment, the sample of 5 × 5 cm of known quality (W) is placed in culture dish.Addition is preheated to the physiology salt of (37 ± 1) DEG C
Water, the additive amount of physiological saline are 40 times, ± 0.5g of material to be tested, are moved into incubator, are kept at (37 ± 1) DEG C
Then 30min clamps one jiao of sample or one end with tweezers, dangle 30s, weighs, and example weight is W1 at this time, and water absorbent rate=
(W1-W) 5 Duplicate Samples are arranged in/W, every group of sample, and experimental result such as Fig. 1 shows.
A kind of high liquid-absorbing fibroin albumen hemostasis obtained by disclosed method through the invention as can see from Figure 1
The absorbent of film Examples 1 to 3 obviously wants high compared with comparative example, and the absorbent of embodiment 1 is 1.49 times of comparative example, imbibition
Specific gravity is 10.21, illustrates that high liquid-absorbing fibroin albumen haemostatic membrane obtained by the present invention has good absorbent.
(2) detection of cytotoxicity
A kind of progress Cytotoxic evaluation experiment of high liquid-absorbing fibroin albumen haemostatic membrane prepared by above-described embodiment 1~3 (is pressed
National standard GB/T 16886.5-2003 is tested), comparative example 1 ~ 3 and comparative example.Experimental result such as Fig. 2 shows.
Cytotoxicity testing result shows embodiment 1-3 its corresponding cell after co-culturing 1 day and 7 days with epithelial cell
Opposite proliferation rate is 85% or more, it was demonstrated that using paradenlal tissue regeneration reparation obtained by preparation method disclosed in this invention
Film has good biocompatibility.
(3) external coagulant property detection:
External coagulant property is detected using Activated partial thromboplastin time (APTT) and blood coagulation protoenzyme time (PT)；
The testing procedure of Activated partial thromboplastin time is as follows: platelet poor plasma and APTT reagent being added in test tube, 37
Then constant temperature 5min at DEG C is added calcium chloride and sample to be tested, is put into APTT test tube, passes through fully automatic blood Solidification Analysis
Instrument collection result；
The testing procedure of blood coagulation protoenzyme time (PT) is as follows: by platelet poor plasma and PT the reagent constant temperature at 37 DEG C respectively
Then 5min is added in PT test tube together, and sample to be tested is added, pass through fully automatic blood Solidification Analysis instrument collection result；
Activated partial thromboplastin time (APTT) clinically refers to that test plasma fibrinogen under calcium ion effect changes
When for insoluble fibrin, the time required for solidifying, influence of the material to intrinsic coagulation system is reflected.Blood coagulation protoenzyme
Time (PT) is exogenous tissue factor generates time of tissue plasminogen activator under calcium ion effect, when tissue by
To when damage, soluble fibrinogen activator can be formed insoluble fibrin clot by the coagulation factor of activation, from
And the loss of blood is prevented, it has reacted the influence of material exogenous blood coagulation system.From the test result of Fig. 3 and Fig. 4 it is found that
Obtained its APTT test result of high liquid-absorbing haemostatic membrane has significant decline, explanation compared to blank group through the invention
Material promotes blood coagulation by acting on the approach of the intrinsic coagulation system of blood, and PT test is without significant change, table
Its bright extrinsic coagulation system for not influencing blood.
(4) hemostasis experiment in vivo
Healthy adult SD rat 20 are taken, is randomly divided into 4 groups, every group 5.Before experiment, 3% yellow Jackets are first used
(3mg/100g) solution carries out vein intraperitoneal injection of anesthesia to SD rat, then shears a length about along ventrimeson with operating scissors
The stringer notch of 2cm successively enters abdominal cavity, exposure liver middle period.Peritoneal fluid around liver is blotted with sterile gauze immediately, in order to avoid
The weighing of peritoneal fluid permeation effects gauze quality.A piece of clean gauze is separately taken to be padded below the liver middle period, with surgical scissors in liver
Middle period lower edge cuts the notch of 2cm, applies sample to be tested hemostasis rapidly, observes and records bleeding stopping period and measure.As a result
It is as shown in the table:
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example | |
| Bleeding stopping period (s) | 121±3.1 | 157±3.2 | 134±2.1 | 160±3.4 |
| Amount of bleeding (g) | 0.34±0.01 | 0.51±0.03 | 0.47±0.04 | 0.64±0.02 |
Table 1- animal hemostasis experimental result
From experimental data it can be seen that high liquid-absorbing fibroin albumen haemostatic membrane obtained by the present invention has the ability of quick-acting haemostatic powder,
Can effectively it stop blooding in a short time.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention；For those of ordinary skill in the art, may be used also on the basis of the above description
To make other variations or changes in different ways, there is no necessity and possibility to exhaust all the enbodiments；It is all this
Made any modifications, equivalent replacements, and improvements etc., should be included in the claims in the present invention within the spirit and principle of invention
Protection scope within.
Claims (8)
1. a kind of high liquid-absorbing fibroin albumen haemostatic membrane, which is characterized in that composed of the following components according to parts by weight: polyoxygenated
5-25 parts of ethylene, 10-50 parts of fibroin albumen, 5-15 parts of sodium alginate, 0.5-1 parts of nano silica.
2. a kind of high liquid-absorbing fibroin albumen haemostatic membrane according to claim 1, which is characterized in that the nanometer two
The particle diameter distribution of silica is 150-250 nm.
3. a kind of high liquid-absorbing fibroin albumen haemostatic membrane according to claim 1, which is characterized in that the fibroin egg
White is as obtained by extracting from silk, and specific extracting method is as follows: the silk raw silk of certain mass is weighed, according to standard proportional
(silk quality/g: distilled water volume/L: natrium carbonicum calcinatum quality/g=10:1:5) weighs distilled water and natrium carbonicum calcinatum；First plus
Enter distilled water, adds natrium carbonicum calcinatum, 100 DEG C of heating, until silk raw silk, heating is added in the inside liquid after boiling completely
30 minutes；After 30min, silk is taken out wash with distilled water, until the clarification of water washed out is transparent；Again according to above step
Heating 30 minutes；After experiment, obtained fibroin albumen is placed in 60 DEG C of baking ovens and is dried for standby.
4. the preparation method of any high liquid-absorbing fibroin albumen haemostatic membrane of claim 1 ~ 3, which is characterized in that described
Preparation method is realized by following steps: (1) preparation of haemostatic membrane spinning material, the spinning of (2) haemostatic membrane, and (3) haemostatic membrane is handed over
Connection processing.
5. the preparation method of high liquid-absorbing fibroin albumen haemostatic membrane according to claim 4, which is characterized in that described stops
The preparation specific steps of blood film spinning material are as follows:
(1) fibroin albumen/nano silica spinning solution preparation: a certain amount of fibroin albumen is weighed by recipe ratio and is dissolved
In trifluoroethanol, it is configured to the solution that mass concentration is 5-10%, nano silica then is added by recipe ratio, under room temperature
2-3h is stirred, it is spare；
(2) polyethylene glycol oxide/sodium alginate spinning solution preparation: weighing a certain amount of polyethylene glycol oxide and be dissolved in distilled water,
It is configured to the polyethylene oxide solutions that mass concentration is 5-10%wt；It weighs a certain amount of sodium alginate to be dissolved in distilled water, match
It is set to the sodium alginate soln that mass concentration is 2-3%；Polyethylene oxide solutions and sodium alginate soln are mixed by recipe ratio,
It stirs evenly, it is spare after defoaming.
6. the preparation method of high liquid-absorbing fibroin albumen haemostatic membrane according to claim 4, which is characterized in that described stops
The preparation specific steps of the spinning of blood film are as follows:
(1) by formula respectively by fibroin albumen/nano silica spinning solution (A liquid) and polyethylene glycol oxide/sodium alginate spinning
Liquid (B liquid) is added in the corresponding syringe of electrostatic spinning machine, is stood 30-60 min, is drained the air of syringe；
(2) adjusting the distance between spinning machine receiver and spinning nozzle is 10-20 cm；High voltage power supply is connected, is adjusted to 10 ~ 20
KV, starts syringe pump, and the output speed for being loaded with the syringe pump of A liquid syringe is adjusted to 0.5-1.5 mL/h, is loaded with B liquid syringe
The output speed of syringe pump be adjusted to 0.6-1.0 mL/h, starting receiver receives spinning, and the revolving speed of receiver is set as 200
~350 rpm；After the spinning solution in wherein one group of syringe exports completely, high voltage power supply is closed, and remove lower spinning film.
7. the preparation method of high liquid-absorbing fibroin albumen haemostatic membrane according to claim 4, which is characterized in that described stops
The crosslinking Treatment specific steps of blood film are as follows: weigh a certain amount of anhydrous calcium chloride and be dissolved in dehydrated alcohol, it is dense to be configured to quality
Degree is the calcium chloride crosslinked fluid of 2-5%wt, obtained spinning film is then soaked in crosslinked fluid 12-24h, then with anhydrous second
Alcohol washs 2-3 times, is placed in 60 DEG C of vacuum drying ovens and dries to get a kind of high liquid-absorbing fibroin albumen haemostatic membrane.
8. the preparation method of high liquid-absorbing fibroin albumen haemostatic membrane according to claim 6, which is characterized in that described
The quantity of syringe is 8-16, and by the difference of the spinning solution filled in syringe, is divided into A group and B group, accordingly, A group note
Emitter is controlled by A syringe pump, and B group syringe is controlled by B syringe pump；The spinning nozzle of the connection of A group syringe corresponds to the spinning of A group
Silk spray head, the spinning nozzle of the connection of B group syringe correspond to B group spinning nozzle, and A group spinning nozzle and B group spinning nozzle are pressed
Sequence alternate is fixed on side by side on the fixed slide unit of spinning nozzle.
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| CN114507384A (en) * | 2022-03-03 | 2022-05-17 | 宁德师范学院 | Silk fibroin/sodium alginate composite membrane, preparation thereof and drug sustained-release carrier material |
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Application publication date: 20190125 |