CN108653718A - A kind of absorbable promoting healing hemostatic composition and dressing - Google Patents
A kind of absorbable promoting healing hemostatic composition and dressing Download PDFInfo
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- CN108653718A CN108653718A CN201810555049.1A CN201810555049A CN108653718A CN 108653718 A CN108653718 A CN 108653718A CN 201810555049 A CN201810555049 A CN 201810555049A CN 108653718 A CN108653718 A CN 108653718A
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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Abstract
The invention discloses a kind of absorbable promoting healing hemostatic composition, the absorbable promoting healing hemostatic composition includes following component：Silver nitrate, vitamin K, vitamin E and epithelical cell growth factor.The invention also discloses a kind of absorbable promoting healing bleeding-stopping dressings and preparation method thereof.Absorbable promoting healing hemostasis combination of the present invention has the effect of preferably hemostasis, promoting healing, it is used in combination with hemostatic material, improve anthemorrhagic speed, the carboxymethyl-cellulose potassium salt gauze prepared in particular by the method for the invention, it is more easy to be absorbed by organisms and without toxicity, and the preparation process of absorbable promoting healing bleeding-stopping dressing of the present invention is simple.
Description
Technical field
The present invention relates to medical supplies fields, and in particular to a kind of absorbable promoting healing hemostatic composition and dressing.
Background technology
Uncontrollability bleeding is to cause war, the most important reason of traffic accident and other Accidents deaths, simultaneously
And the problem often to be faced in emergency care of trauma and surgical operation.There are many topical hemostatic agents to be applied to clinic,
These hemostatic materials mainly have the products such as gelfoam, collagen protein sponge, Fibrin Glue, oxycellulose.Gelfoam
It clinically applies for many years, but its hydrophily is poor, for larger bleeding, its haemostatic effect is not good enough.Collagen protein sponge stops
Blood performance ratio gelfoam is good, and raw materials for production come from ox heel string, tendon, therefore similarly there are viral disease transmissions
It is dangerous.Fibrin Glue in clinical operation hemostasis using relatively broad, but since fibrin glue component belongs to blood product,
Therefore there is the danger of certain antigenicity and viral disease transmission, application security is by certain query.It is above-mentioned
Gelfoam, collagen protein sponge, Fibrin Glue belong to heterogenous animal albumen, have certain immunogenicity and virus to pass
The danger broadcast, while increasing the infection chance of the surface of a wound.Therefore a kind of non-immunogenicity, virus-free infectiousness are developed, and had
It is necessary that antibacterial heal-promoting, which closes degradable hemostatic gauze,.
Invention content
A kind of absorbable promoting healing hemostasis is provided it is an object of the invention to overcome the shortcomings of the prior art place
Composition and dressing.
To achieve the above object, the technical solution that the present invention takes is：A kind of absorbable promoting healing hemostatic composition, including
Following component：Silver nitrate, vitamin K, vitamin E and epithelical cell growth factor.
Epithelical cell growth factor in the present invention is using epithelical cell growth factor freeze-dried powder.
As the preferred embodiment of absorbable promoting healing hemostatic composition of the present invention, the absorbable promoting healing stops
Blood composition includes the component of following parts by weight：Silver nitrate 3 × 10-6~7 × 10-6Part, vitamin K1~4 part, vitamin E
0.5~2 part and 0.065~0.1 part of epithelical cell growth factor.
Absorbable promoting healing hemostatic composition of the present invention can have preferable antibacterial, rush using the component of said ratio
Into wound healing, the fast effect of anthemorrhagic speed.
As the preferred embodiment of absorbable promoting healing hemostatic composition of the present invention, the absorbable promoting healing stops
Blood composition includes the component of following parts by weight：Silver nitrate 4 × 10-6~6 × 10-6Part, farnoquinone~3 part, vitamin E
0.8~1.5 part and 0.07~0.09 part of epithelical cell growth factor.
As the preferred embodiment of absorbable promoting healing hemostatic composition of the present invention, the absorbable promoting healing stops
Blood composition includes the component of following parts by weight：Silver nitrate 5 × 10-6Part, 2.5 parts of vitamin K, 1 part of vitamin E and epidermis are thin
0.08 part of the intracellular growth factor.
The present invention also aims to provide a kind of absorbable promoting healing bleeding-stopping dressing, including adsorption substrates and described inhale
Receive promoting healing hemostatic composition；The absorbable promoting healing hemostatic composition is attached in adsorption substrates.
The absorbable promoting healing hemostatic composition is attached in adsorption substrates, hemostasis can be preferably played and promote more to imitate
Fruit.
As the preferred embodiment of absorbable promoting healing bleeding-stopping dressing of the present invention, the absorbable promoting healing hemostasis
Weight percentage of the composition in the absorbable promoting healing bleeding-stopping dressing is 0.1~5%.The absorbable promoting healing stops
Blood composition can play antibacterial and promote the effect of wound healing when the absorbable promoting healing bleeding-stopping dressing is above-mentioned content
Fruit.
As the preferred embodiment of absorbable promoting healing bleeding-stopping dressing of the present invention, the absorbable promoting healing hemostasis
Weight percentage of the composition in the absorbable promoting healing bleeding-stopping dressing is 1~3%.Especially in above-mentioned content,
The utilization ratio higher of the absorbable promoting healing hemostatic composition.
As the preferred embodiment of absorbable promoting healing bleeding-stopping dressing of the present invention, the absorbable promoting healing hemostasis
Weight percentage of the composition in the absorbable promoting healing bleeding-stopping dressing is 2%.The absorbable promoting healing hemostasis exists
When above-mentioned content, the utilization ratio highest of the absorbable promoting healing hemostatic composition.
As the preferred embodiment of absorbable promoting healing bleeding-stopping dressing of the present invention, the adsorption substrates are oxygen regeneration
Cellulose gauze, carboxyethyl cellulose gauze or carboxymethyl cellulose gauze.
As the preferred embodiment of absorbable promoting healing bleeding-stopping dressing of the present invention, the adsorption substrates are carboxymethyl
Cellulose gauze.
As the preferred embodiment of absorbable promoting healing bleeding-stopping dressing of the present invention, the adsorption substrates are carboxymethyl
Cellulose sylvite gauze.
Carboxymethyl-cellulose potassium salt gauze is used in combination with absorbable promoting healing hemostatic composition, can improve hemostasis speed
Degree, more effectively promotes wound healing.
The present invention also aims to provide a kind of preparation method of absorbable promoting healing bleeding-stopping dressing, the absorbable rush
The preparation method of the dressing of healing hemostasis includes the following steps：
(1), it prepares and can absorb promoting healing hemostatic composition solution：The absorbable promoting healing hemostatic composition is added double
It steams in water；
(2), adsorption substrates are soaked in 1~3h in absorbable promoting healing hemostatic composition solution；
(3), it takes out, cleaning, it is dry to get the absorbable promoting healing bleeding-stopping dressing.
The preferred embodiment of preparation method as absorbable promoting healing bleeding-stopping dressing of the present invention, the absorption base
Material is carboxymethyl-cellulose potassium salt gauze；
The preparation method of the carboxymethyl-cellulose potassium salt gauze includes the following steps：
(a), it pre-processes：By degreasing cotton gauze in the distilled water that temperature is 55~85 DEG C, 1~4h is washed, must be pre-processed
The degreasing cotton gauze crossed；
(b), basification：Degreasing cotton gauze pretreated obtained by (a) is subjected to basification, after obtaining basification
Degreasing cotton gauze；
(c), etherification process：Monoxone is added in degreasing cotton gauze after the basification obtained by (b) and carries out etherification process,
Obtain the degreasing cotton gauze after etherification process；
(d), alcohol is washed and is neutralized：Degreasing cotton gauze after etherification process obtained by (c) is washed repeatedly with methanol, is added dropwise simultaneously
Glacial acetic acid adjusts acid-base value, and it is 6.5~7.5 to make its pH；
(f), by the gauze freeze-drying process of (d) processing, the carboxymethyl-cellulose potassium salt gauze is obtained.
It is easier to be absorbed by organisms using carboxymethyl-cellulose potassium salt gauze made from the above method, can be used for internal wound
Hemostasis, and have no toxic side effect.
The preferred embodiment of preparation method as absorbable promoting healing bleeding-stopping dressing of the present invention, in step (b),
The process of the basification is：It is that 10~20%KOH is molten that pretreated degreasing cotton gauze, which is soaked in mass percentage,
In liquid, the weight ratio of the degreasing cotton gauze and KOH solution is：Degreasing cotton gauze：KOH solution=1：40~55, use acetone
As diluent, quaternization is carried out, 3~6h is reacted under the conditions of stirring or ultrasonic vibration at a temperature of -20~37 DEG C, obtains alkali
Change treated degreasing cotton gauze.
The preferred embodiment of preparation method as absorbable promoting healing bleeding-stopping dressing of the present invention, in step (c),
Degreasing cotton gauze and chloroacetic weight ratio after the basification are：Degreasing cotton gauze after basification：Monoxone
=1:60~100, the reaction condition of the etherification process is：Be 1~2 DEG C in heating rate, be warming up at 65 DEG C reaction 2~
3h is continuously heating to 85 DEG C, 1~2h of constant temperature.
The preferred embodiment of preparation method as absorbable promoting healing bleeding-stopping dressing of the present invention, in step (3),
To be cleaned using methanol solution, the drying is by the way of freeze-drying for the cleaning.
The beneficial effects of the present invention are：The present invention provides a kind of absorbable promoting healing hemostatic composition and dressing.This
Inventing the absorbable promoting healing hemostasis combination has the effect of preferably hemostasis, promoting healing, is used in combination, carries with hemostatic material
High anthemorrhagic speed is more easy to be inhaled by body in particular by carboxymethyl-cellulose potassium salt gauze prepared by the method for the invention
It receives and without toxicity, and the preparation process of absorbable promoting healing bleeding-stopping dressing of the present invention is simple.
Specific implementation mode
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of embodiment of absorbable promoting healing hemostatic composition of the present invention can absorb promoting healing described in the present embodiment
Hemostatic composition includes following components：5 μ g of silver nitrate, vitamin K 2.5g, vitamin E 1g and epithelical cell growth factor
0.08g。
A kind of absorbable promoting healing bleeding-stopping dressing, including promoting healing hemostasis group is can absorb described in adsorption substrates and the present embodiment
Close object；The absorbable promoting healing hemostatic composition is attached in adsorption substrates, and the adsorption substrates are potassium carboxymethylcellulose
Salt gauze, weight percentage of the absorbable promoting healing hemostatic composition in the absorbable promoting healing bleeding-stopping dressing are
2%.
The preparation method that the dressing of promoting healing hemostasis is can absorb described in the present embodiment includes the following steps：
(1), it prepares and can absorb promoting healing hemostatic composition solution：The absorbable promoting healing hemostatic composition is dissolved in
In 30mL distilled waters；
(2), carboxymethyl-cellulose potassium salt gauze is soaked in 2h in absorbable promoting healing hemostatic composition solution；
(3), it takes out, is washed repeatedly, be freeze-dried to get the absorbable promoting healing bleeding-stopping dressing repeatedly with methanol；
Wherein, the preparation method of the carboxymethyl-cellulose potassium salt gauze includes the following steps：
(a), it pre-processes：By degreasing cotton gauze in the distilled water that temperature is 80 DEG C, 2h is washed, pretreated degreasing is obtained
Cotton gauze；
(b), basification：It is 18%KOH that degreasing cotton gauze pretreated obtained by (a), which is soaked in mass percentage,
In solution, the weight ratio of the degreasing cotton gauze and KOH solution is：Degreasing cotton gauze：KOH solution=1：50, made with acetone
For diluent, quaternization is carried out, 5h is reacted under the conditions of stirring or ultrasonic vibration at a temperature of 25 DEG C, after obtaining basification
Degreasing cotton gauze；
(c), etherification process：Monoxone is added in degreasing cotton gauze after the basification obtained by (b) and carries out etherification process,
Degreasing cotton gauze and chloroacetic weight ratio after basification are：Degreasing cotton gauze after basification：Monoxone=1:
80, it is 1.5 DEG C in heating rate, is warming up at 65 DEG C and reacts 2.5h, be continuously heating to 85 DEG C, constant temperature 1.5h obtains etherification process
Degreasing cotton gauze afterwards；
(d), alcohol is washed and is neutralized：Degreasing cotton gauze after etherification process obtained by (c) is washed repeatedly with methanol, is added dropwise simultaneously
Glacial acetic acid adjusts acid-base value, and it is 6.5~7.5 to make its pH；
(f), by the gauze freeze-drying process of (d) processing, carboxymethyl-cellulose potassium salt gauze is obtained.
Embodiment 2
A kind of embodiment of absorbable promoting healing hemostatic composition of the present invention can absorb promoting healing described in the present embodiment
Hemostatic composition includes following components：3 μ g of silver nitrate, vitamin K 1g, vitamin E 0.5g and epithelical cell growth factor
0.065 part.
A kind of absorbable promoting healing bleeding-stopping dressing, including promoting healing hemostasis group is can absorb described in adsorption substrates and the present embodiment
Close object；The absorbable promoting healing hemostatic composition is attached in adsorption substrates, and the adsorption substrates are potassium carboxymethylcellulose
Salt gauze, weight percentage of the absorbable promoting healing hemostatic composition in the absorbable promoting healing bleeding-stopping dressing are
2%.
The preparation method that the dressing of promoting healing hemostasis is can absorb described in the present embodiment includes the following steps：
(1), it prepares and can absorb promoting healing hemostatic composition solution：The absorbable promoting healing hemostatic composition is dissolved in
In 30mL distilled waters；
(2), carboxymethyl-cellulose potassium salt gauze is soaked in 1h in absorbable promoting healing hemostatic composition solution；
(3), it takes out, is washed repeatedly, be freeze-dried to get the absorbable promoting healing bleeding-stopping dressing repeatedly with methanol；
Wherein, the preparation method of the carboxymethyl-cellulose potassium salt gauze includes the following steps：
(a), it pre-processes：By degreasing cotton gauze in the distilled water that temperature is 55 DEG C, 1h is washed, pretreated degreasing is obtained
Cotton gauze；
(b), basification：It is 10%KOH that degreasing cotton gauze pretreated obtained by (a), which is soaked in mass percentage,
In solution, the weight ratio of the degreasing cotton gauze and KOH solution is：Degreasing cotton gauze：KOH solution=1：40, made with acetone
For diluent, quaternization is carried out, 3h is reacted under the conditions of stirring or ultrasonic vibration at a temperature of -20 DEG C, after obtaining basification
Degreasing cotton gauze；
(c), etherification process：Monoxone is added in degreasing cotton gauze after the basification obtained by (b) and carries out etherification process,
Degreasing cotton gauze and chloroacetic weight ratio after basification are：Degreasing cotton gauze after basification：Monoxone=1:
60, it is 1 DEG C in heating rate, is warming up at 65 DEG C and reacts 2h, be continuously heating to 85 DEG C, constant temperature 1h, obtains de- after etherification process
Fat cotton gauze；
(d), alcohol is washed and is neutralized：Degreasing cotton gauze after etherification process obtained by (c) is washed repeatedly with methanol, is added dropwise simultaneously
Glacial acetic acid adjusts acid-base value, and it is 6.5~7.5 to make its pH；
(f), by the gauze freeze-drying process of (d) processing, carboxymethyl-cellulose potassium salt gauze is obtained.
Embodiment 3
A kind of embodiment of absorbable promoting healing hemostatic composition of the present invention can absorb promoting healing described in the present embodiment
Hemostatic composition includes following components：7 μ g of silver nitrate, vitamin K 4g, vitamin E 2g and epithelical cell growth factor 0.1g.
A kind of absorbable promoting healing bleeding-stopping dressing, including promoting healing hemostasis group is can absorb described in adsorption substrates and the present embodiment
Close object；The absorbable promoting healing hemostatic composition is attached in adsorption substrates, and the adsorption substrates are potassium carboxymethylcellulose
Salt gauze, weight percentage of the absorbable promoting healing hemostatic composition in the absorbable promoting healing bleeding-stopping dressing are
2%.
The preparation method that the dressing of promoting healing hemostasis is can absorb described in the present embodiment includes the following steps：
(1), it prepares and can absorb promoting healing hemostatic composition solution：The absorbable promoting healing hemostatic composition is dissolved in
In 30mL distilled waters；
(2), carboxymethyl-cellulose potassium salt gauze is soaked in 3h in absorbable promoting healing hemostatic composition solution；
(3), it takes out, is washed repeatedly, be freeze-dried to get the absorbable promoting healing bleeding-stopping dressing repeatedly with methanol；
Wherein, the preparation method of the carboxymethyl-cellulose potassium salt gauze includes the following steps：
(a), it pre-processes：By degreasing cotton gauze in the distilled water that temperature is 85 DEG C, 4h is washed, pretreated degreasing is obtained
Cotton gauze；
(b), basification：It is 20%KOH that degreasing cotton gauze pretreated obtained by (a), which is soaked in mass percentage,
In solution, the weight ratio of the degreasing cotton gauze and KOH solution is：Degreasing cotton gauze：KOH solution=1：55, made with acetone
For diluent, quaternization is carried out, 6h is reacted under the conditions of stirring or ultrasonic vibration at a temperature of 37 DEG C, after obtaining basification
Degreasing cotton gauze；
(c), etherification process：Monoxone is added in degreasing cotton gauze after the basification obtained by (b) and carries out etherification process,
Degreasing cotton gauze and chloroacetic weight ratio after basification are：Degreasing cotton gauze after basification：Monoxone=1:
100, it is 2 DEG C in heating rate, is warming up at 65 DEG C and reacts 3h, be continuously heating to 85 DEG C, constant temperature 2h, obtains de- after etherification process
Fat cotton gauze；
(d), alcohol is washed and is neutralized：Degreasing cotton gauze after etherification process obtained by (c) is washed repeatedly with methanol, is added dropwise simultaneously
Glacial acetic acid adjusts acid-base value, and it is 6.5~7.5 to make its pH；
(f), by the gauze freeze-drying process of (d) processing, carboxymethyl-cellulose potassium salt gauze is obtained.
Embodiment 4
A kind of embodiment of absorbable promoting healing hemostatic composition of the present invention can absorb promoting healing described in the present embodiment
Hemostatic composition includes following components：4 μ g of silver nitrate, vitamin K 2g, vitamin E 0.8g and epithelical cell growth factor
0.07g。
A kind of absorbable promoting healing bleeding-stopping dressing, including promoting healing hemostasis group is can absorb described in adsorption substrates and the present embodiment
Close object；The absorbable promoting healing hemostatic composition is attached in adsorption substrates, and the adsorption substrates are potassium carboxymethylcellulose
Salt gauze, weight percentage of the absorbable promoting healing hemostatic composition in the absorbable promoting healing bleeding-stopping dressing are
2%.
The dressing that promoting healing stops blooding is can absorb described in the present embodiment, and the preparation method is the same as that of Example 1.
Embodiment 5
A kind of embodiment of absorbable promoting healing hemostatic composition of the present invention can absorb promoting healing described in the present embodiment
Hemostatic composition includes following components：6 μ g of silver nitrate, vitamin K 3g, vitamin E 1.5g and epithelical cell growth factor
0.09g。
A kind of absorbable promoting healing bleeding-stopping dressing, including promoting healing hemostasis group is can absorb described in adsorption substrates and the present embodiment
Close object；The absorbable promoting healing hemostatic composition is attached in adsorption substrates, and the adsorption substrates are potassium carboxymethylcellulose
Salt gauze, weight percentage of the absorbable promoting healing hemostatic composition in the absorbable promoting healing bleeding-stopping dressing are
2%.
The dressing that promoting healing stops blooding is can absorb described in the present embodiment, and the preparation method is the same as that of Example 1.
Embodiment 6
The cytotoxicity detection of promoting healing bleeding-stopping dressing is can absorb described in Examples 1 to 5, operating procedure is as follows：
(1) preparation of each gauze leaching liquor：It is yarn that promoting healing bleeding-stopping dressing and speed will be can absorb described in Examples 1 to 5
(Johnson ＆ Johnson of the U.S.) presses 0.1g/mL extractions again after extracting the DMEM culture mediums saturation for absorbing 10% fetal calf serum, and 37 DEG C extract for 24 hours,
Leaching liquor is as test sample group；
(2) prepared by cell：Recovery freezes L929 cells, after reaching for 2 generations, prepares 1 × 104A/mL cell suspensions are added extremely
Per 200 μ L of hole in 96 orifice plates, it is positioned over 37 DEG C, in 5% carbon dioxide incubator, culture is for 24 hours；
(3) it is loaded：By cultured cell, original fluid is discarded, PBS washes away dead cell, and 200 μ L are added per hole for experiment
Product, three, each sample are parallel；
(4) it detects：(3) are positioned over 37 DEG C, in 5% carbon dioxide incubator, after culture for 24 hours, are detected using mtt assay thin
Cellular toxicity.
Test result is as shown in table 1.
The cytotoxicity testing result of promoting healing bleeding-stopping dressing is can absorb described in 1 Examples 1 to 5 of table
By the test result of table 1 it is found that it is that yarn is nontoxic to can absorb promoting healing bleeding-stopping dressing and speed described in Examples 1 to 5
Property, and absorbable promoting healing bleeding-stopping dressing described in Examples 1 to 5 can promote the effect of cell growth, the wherein effect of embodiment 1
It is the most apparent.
Experimental example 7
The bleeding stopping period detection of promoting healing bleeding-stopping dressing, concrete operation step are can absorb described in Examples 1 to 5：
(1) 12 healthy new zealand white rabbits are chosen, auricular vein disinfection is slowly injected 3% Nembutal sodium solution, waited for
It is tested after its holonarcosis；
(2) it chooses rabbit ear central aorta and then uses scalpel with Iodophor to carrying out disinfection at rabbit ear artery
Make the surface of a wound of 1 × 1cm；
(3) after the bleeding of rabbit ear artery, blood first is absorbed with common sterile gauze, it can by what is prepared in Examples 1 to 5
The size that absorption promoting healing bleeding-stopping dressing is cut into 2 × 2cm spreads on the bleeding surface of a wound；
(4) surface of a wound is absorbed with common sterile gauze per 20s and ooze out blood, observe haemostatic effect；
(5) with speed i.e. yarn (Johnson ＆ Johnson of the U.S.) for control group, each dressing does three parallel laboratory tests, respectively record hemostasis completely
Time calculates average.
Test result is as shown in table 2.
The bleeding stopping period testing result of promoting healing bleeding-stopping dressing is can absorb described in 2 Examples 1 to 5 of table
As seen from the results in Table 2, the bleeding stopping period for promoting healing bleeding-stopping dressing being can absorb described in Examples 1 to 5 is respectively less than speed i.e.
Yarn and significant difference (P<0.05), and 1 bleeding stopping period of embodiment is most short.
Experimental example 8
The degradation speed detection of promoting healing bleeding-stopping dressing, concrete operation step are can absorb described in Examples 1 to 5：
(1) 12 healthy new zealand white rabbits are chosen, auricular vein disinfection is slowly injected 3% Nembutal sodium solution, waited for
It is tested after its holonarcosis；
(2) rabbit ear central aorta is chosen, shaving disinfection is carried out to rabbit huckle with Iodophor, then uses scalpel
Make the surface of a wound of 1cm；
(3) after bleeding, blood first is absorbed with common sterile gauze, promoting healing hemostasis will be can absorb described in Examples 1 to 5 and is applied
Material is cut into 1 × 2cm strips, is put in wound, sutures the surface of a wound afterwards；
(4) variation of observation abdominal wound daily, takes out stitches after 8d, observes the degradation situation of each gauze.
(5) with speed i.e. yarn (Johnson ＆ Johnson of the U.S.) for control group, each dressing does three parallel laboratory tests, records each dressing respectively
Surplus calculates average.
Test result is as shown in table 3.
The degradation speed test result of promoting healing bleeding-stopping dressing is can absorb described in 3 Examples 1 to 5 of table
| Sample sets | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Speed is yarn |
| Each gauze surplus (%) | 0 | 3 | 5 | 1 | 2 | 10 |
As seen from the results in Table 3, the degradation speed that promoting healing bleeding-stopping dressing gauze is can absorb described in Examples 1 to 5 is all higher than
Speed is yarn significant difference (P<0.05), and the degradation speed of embodiment 1 is most fast.
Embodiment 9
For the proportioning for probing into absorbable promoting healing hemostatic composition and the effect for promoting wound healing, hemostasis, table 4 is set
In 17 test groups and 4 control groups, the absorbable promoting healing hemostatic composition of test group 1~17 and control group 1~4 is pressed
The preparation method that promoting healing bleeding-stopping dressing is can absorb described in embodiment 1 is prepared into absorbable promoting healing bleeding-stopping dressing, and test group 1~
17 and 1~4 absorbable promoting healing hemostatic composition of control group the sum of total amount with embodiment 1, testing experiment group 1~17 and control
The effect for promoting wound healing, hemostasis of group 1~4, promotes the test method of wound healing effect with embodiment 6, haemostatic effect
Test method with embodiment 7.
The absorbable promoting healing hemostatic composition of the different compositions of table 4
Test result is shown in Table 5.
The test result of the promotion cell growth and bleeding stopping period of the absorbable promoting healing hemostatic composition of the different compositions of table 5
The absorbable promoting healing hemostatic composition is can be seen that using the proportioning in test group 1 from the test result of table 5
When, vitro growth rates and anthemorrhagic speed are best, the vitro growth rates and hemostasis speed of test group 3,4,7,8,11,12,15,16
Degree is better than test group 2,5,6,9,10,14,17, and is superior to control 1~4, illustrates that absorbable promoting healing hemostatic composition only has
The effect of promoting healing and hemostatic and antibacterial could be preferably played under certain proportioning.
Embodiment 10
In order to probe into effect of the content of absorbable promoting healing hemostatic composition in dressings to promotion wound healing, hemostasis
5 test groups in table 6 and 2 control groups are arranged in fruit, and the absorbable promoting healing of test group 1~5 and control group 1,2 is stopped blooding
Composition is prepared into absorbable promoting healing bleeding-stopping dressing by the composition and preparation method of embodiment 1, testing experiment group 1~5 and right
According to the effect for promoting wound healing, hemostasis of group 1,2, promote the test method of wound healing effect with embodiment 6, haemostatic effect
Test method with embodiment 7.
Table 6 can absorb the test result of the promotion cell growth and bleeding stopping period of promoting healing hemostatic composition different content
From the results shown in Table 6, when the absorbable weight percentage of promoting healing hemostatic composition in dressings is
When 0.1%, the absorbable promoting healing hemostatic composition, which can effectively shorten, bleeding stopping period and promotes wound healing, with absorbable
The increase of promoting healing hemostatic composition additive amount, anthemorrhagic speed and promoting healing effect increase, but absorbable promoting healing hemostasis
When the additive amount of composition in dressings is more than 2%, it is further added by the content of absorbable promoting healing hemostatic composition, anthemorrhagic speed
With promote wound healing it is effect improved not significantly.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of range is protected, although being explained in detail to the present invention with reference to preferred embodiment, those skilled in the art should
Understand, technical scheme of the present invention can be modified or replaced equivalently, without departing from the essence of technical solution of the present invention
And range.
Claims (10)
1. a kind of absorbable promoting healing hemostatic composition, which is characterized in that include following component：Silver nitrate, vitamin K, dimension life
Plain E and epithelical cell growth factor.
2. absorbable promoting healing hemostatic composition as described in claim 1, which is characterized in that the absorbable promoting healing hemostasis group
Close the component that object includes following parts by weight：Silver nitrate 3 × 10-6~7 × 10-6Part, vitamin K1~4 part, vitamin E 0.5~2
0.065~0.1 part of part and epithelical cell growth factor.
3. absorbable promoting healing hemostatic composition as described in claim 1, which is characterized in that the absorbable promoting healing hemostasis group
Close the component that object includes following parts by weight：Silver nitrate 4 × 10-6~6 × 10-6Part, farnoquinone~3 part, vitamin E 0.8~
1.5 parts and 0.07~0.09 part of epithelical cell growth factor；Preferably, the absorbable promoting healing hemostatic composition includes following
The component of parts by weight：Silver nitrate 5 × 10-6Part .5 parts of farnoquinone, 1 part of vitamin E and 0.08 part of epithelical cell growth factor.
4. a kind of absorbable promoting healing bleeding-stopping dressing, which is characterized in that including any one of adsorption substrates and claims 1 to 3
The absorbable promoting healing hemostatic composition；The absorbable promoting healing hemostatic composition is attached in adsorption substrates.
5. absorbable promoting healing bleeding-stopping dressing as claimed in claim 4, which is characterized in that the absorbable promoting healing hemostasis combination
Weight percentage of the object in the absorbable promoting healing bleeding-stopping dressing is 0.1~5%；Preferably, the absorbable rush is cured
It is 1~3% to close weight percentage of the hemostatic composition in the absorbable promoting healing bleeding-stopping dressing；It is highly preferred that described
Weight percentage of the absorbable promoting healing hemostatic composition in the absorbable promoting healing bleeding-stopping dressing is 2%.
6. absorbable promoting healing bleeding-stopping dressing as claimed in claim 4, which is characterized in that the adsorption substrates are oxygen regeneration chemical fibre
The plain gauze of dimension, carboxyethyl cellulose gauze or carboxymethyl cellulose gauze；Preferably, the adsorption substrates are carboxymethyl cellulose
Gauze；It is highly preferred that the adsorption substrates are carboxymethyl-cellulose potassium salt gauze.
7. a kind of preparation method of absorbable promoting healing bleeding-stopping dressing, which is characterized in that the absorbable promoting healing hemostasis is applied
The preparation method of material includes the following steps：
(1), it prepares and can absorb promoting healing hemostatic composition solution：By any one of claims 1 to 3 absorbable promoting healing
Hemostatic composition is added in distilled water；
(2), adsorption substrates are soaked in 1~3h in absorbable promoting healing hemostatic composition solution；
(3), it takes out, cleaning, it is dry to get the absorbable promoting healing bleeding-stopping dressing.
8. the preparation method of absorbable promoting healing bleeding-stopping dressing as claimed in claim 7, which is characterized in that the adsorption substrates are
Carboxymethyl-cellulose potassium salt gauze；
The preparation method of the carboxymethyl-cellulose potassium salt gauze includes the following steps：
(a), it pre-processes：By degreasing cotton gauze in the distilled water that temperature is 55~85 DEG C, 1~4h is washed, is obtained pretreated
Degreasing cotton gauze；
(b), basification：Degreasing cotton gauze pretreated obtained by (a) is subjected to basification, obtains the degreasing after basification
Cotton gauze；
(c), etherification process：Monoxone is added in degreasing cotton gauze after the basification obtained by (b) and carries out etherification process, obtains ether
Change treated degreasing cotton gauze；
(d), alcohol is washed and is neutralized：Degreasing cotton gauze after etherification process obtained by (c) is washed repeatedly with methanol, while ice vinegar is added dropwise
Acid adjusts acid-base value, and it is 6.5~7.5 to make its pH；
(f), by the gauze freeze-drying process of (d) processing, the carboxymethyl-cellulose potassium salt gauze is obtained.
9. the preparation method of absorbable promoting healing bleeding-stopping dressing as claimed in claim 8, which is characterized in that described in step (b)
The process of basification is：By pretreated degreasing cotton gauze be soaked in mass percentage be 10~20%KOH solution in,
The degreasing cotton gauze and the weight ratio of KOH solution are：Degreasing cotton gauze：KOH solution=1：40~55, use acetone as dilute
Agent is released, quaternization is carried out, 3~6h is reacted under the conditions of stirring or ultrasonic vibration at a temperature of -20~37 DEG C, obtains basification
Degreasing cotton gauze afterwards.
10. the preparation method of absorbable promoting healing bleeding-stopping dressing as claimed in claim 8, which is characterized in that in step (c), institute
It states the degreasing cotton gauze after basification and chloroacetic weight ratio is：Degreasing cotton gauze after basification：Monoxone=
1:60~100, the reaction condition of the etherification process is：It is 1~2 DEG C in heating rate, is warming up to 2~3h of reaction at 65 DEG C,
85 DEG C are continuously heating to, 1~2h of constant temperature.
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| CN112823029A (en) * | 2018-10-17 | 2021-05-18 | 东丽株式会社 | Hemostatic material |
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