CN108653718B - Absorbable healing-promoting hemostatic composition and dressing - Google Patents
Absorbable healing-promoting hemostatic composition and dressing Download PDFInfo
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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Abstract
The invention discloses an absorbable healing-promoting hemostatic composition, which comprises the following components: silver nitrate, vitamin K, vitamin E and epidermal growth factor. The invention also discloses an absorbable healing-promoting hemostatic dressing and a preparation method thereof. The absorbable healing-promoting hemostatic composition has better hemostatic and healing-promoting effects, is used together with a hemostatic material to improve the hemostatic speed, and particularly, the carboxymethyl cellulose sylvite gauze prepared by the method is easier to absorb by organisms and has no toxic or side reaction, and the preparation process of the absorbable healing-promoting hemostatic dressing is simple.
Description
Technical Field
The invention relates to the field of medical supplies, in particular to an absorbable healing-promoting hemostatic composition and a dressing.
Background
Uncontrolled bleeding is the most important cause of war, traffic accident and other accidental injuries and is also a problem often faced in first aid trauma and surgical procedures. At present, various local hemostatic materials are applied to clinic, and the hemostatic materials mainly comprise products such as gelatin sponge, collagen sponge, fibrin glue, oxidized cellulose and the like. The gelatin sponge is clinically applied for years, but the hydrophilicity is poor, and the hemostatic effect is not good enough for the major hemorrhage. The collagen sponge has better hemostatic performance than gelatin sponge, and the raw materials for producing the collagen sponge come from bovine achilles tendon and tendon, so the collagen sponge also has the risk of viral disease transmission. Fibrin glue is widely applied in clinical operation hemostasis, but because the fibrin glue component belongs to blood products, certain antigenicity and viral disease transmission risk exist, and the application safety is questioned to a certain extent. The gelatin sponge, the collagen sponge and the fibrin glue belong to heterogeneous animal proteins, have certain immunogenicity and virus transmission risks, and increase the infection chance of the wound surface. Therefore, it is necessary to develop a hemostatic gauze which has no immunogenicity, no virus infectivity, and antibacterial, healing-promoting and degradable properties.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide an absorbable healing-promoting hemostatic composition and dressing.
In order to achieve the purpose, the invention adopts the technical scheme that: an absorbable healing promoting hemostatic composition comprising the following ingredients: silver nitrate, vitamin K, vitamin E and epidermal growth factor.
The epidermal cell growth factor in the invention is epidermal cell growth factor freeze-dried powder.
As a preferred embodiment of the absorbable healing promoting hemostatic composition of the present invention, the absorbable healing promoting hemostatic composition comprises the following components in parts by weight: silver nitrate 3X 10-6～7×10-6The vitamin K supplement comprises, by weight, 1-4 parts of vitamin K, 0.5-2 parts of vitamin E and 0.065-0.1 part of epidermal growth factor.
The absorbable healing-promoting hemostatic composition prepared from the components according to the proportion has the effects of better antibiosis, wound healing promotion and high hemostatic speed.
As a preferred embodiment of the absorbable healing promoting hemostatic composition of the present invention, the absorbable healing promoting hemostatic composition comprises the following components in parts by weight: silver nitrate 4X 10-6～6×10-6The vitamin K feed additive comprises, by weight, vitamin K2-3 parts, vitamin E0.8-1.5 parts and epidermal growth factor 0.07-0.09 part.
As a preferred embodiment of the absorbable healing promoting hemostatic composition of the present invention, the absorbable healing promoting hemostatic composition comprises the following components in parts by weight: silver nitrate 5X 10-6Portions and vitamins2.5 parts of vitamin K, 1 part of vitamin E and 0.08 part of epidermal growth factor.
It is also an object of the present invention to provide an absorbable healing promoting hemostatic dressing comprising an absorbent substrate and the absorbable healing promoting hemostatic composition; the absorbable healing promoting hemostatic composition is attached to an absorbent substrate.
The absorbable healing-promoting hemostatic composition is attached to an adsorption base material, so that the hemostatic and healing-promoting effects can be better exerted.
As a preferable embodiment of the absorbable wound healing promoting hemostatic dressing, the absorbable wound healing promoting hemostatic dressing comprises 0.1-5 wt% of the absorbable wound healing promoting hemostatic dressing. The absorbable healing promoting hemostatic composition can play the roles of resisting bacteria and promoting wound healing when the content of the absorbable healing promoting hemostatic dressing is the content.
As a preferable embodiment of the absorbable wound healing promoting hemostatic dressing, the absorbable wound healing promoting hemostatic dressing comprises 1-3 wt% of the absorbable wound healing promoting hemostatic dressing. Particularly at the above levels, the absorbable healing promoting hemostatic composition is more efficiently utilized.
As a preferred embodiment of the absorbable healing promoting hemostatic dressing of the present invention, the absorbable healing promoting hemostatic composition is present in the absorbable healing promoting hemostatic dressing in an amount of 2% by weight. At the above levels, the absorbable healing promoting hemostatic composition is most efficiently utilized.
As a preferred embodiment of the absorbable healing-promoting hemostatic dressing of the present invention, the absorbent substrate is a regenerated oxidized cellulose gauze, a carboxyethyl cellulose gauze or a carboxymethyl cellulose gauze.
As a preferred embodiment of the absorbable healing-promoting hemostatic dressing of the present invention, the absorbent substrate is a carboxymethyl cellulose gauze.
As a preferred embodiment of the absorbable healing-promoting hemostatic dressing of the present invention, the absorbent substrate is a carboxymethyl cellulose potassium salt gauze.
The carboxymethyl cellulose sylvite gauze and the absorbable healing-promoting hemostatic composition are used together, so that the hemostatic speed can be improved, and the wound healing can be effectively promoted.
The invention also aims to provide a preparation method of the absorbable healing-promoting hemostatic dressing, which comprises the following steps:
(1) preparing an absorbable healing-promoting hemostatic composition solution: adding the absorbable healing promoting hemostatic composition to double distilled water;
(2) soaking the adsorption substrate in the absorbable healing-promoting and hemostatic composition solution for 1-3 hours;
(3) and taking out, cleaning and drying to obtain the absorbable healing-promoting hemostatic dressing.
As a preferred embodiment of the preparation method of the absorbable healing-promoting hemostatic dressing of the invention, the absorption substrate is carboxymethyl cellulose potassium salt gauze;
the preparation method of the carboxymethyl cellulose potassium salt gauze comprises the following steps:
(a) and (3) pretreatment: washing absorbent cotton gauze in double-distilled water at the temperature of 55-85 ℃ for 1-4 h to obtain pretreated absorbent cotton gauze;
(b) and alkalization treatment: performing alkalization treatment on the pretreated absorbent cotton gauze obtained in the step (a) to obtain alkalized absorbent cotton gauze;
(c) and (3) etherification treatment: adding chloroacetic acid into the alkalized absorbent cotton gauze obtained in the step (b) for etherification to obtain the etherified absorbent cotton gauze;
(d) alcohol washing and neutralization: washing the etherified absorbent cotton gauze obtained in the step (c) for multiple times by using methanol, and simultaneously dropwise adding glacial acetic acid to adjust the pH value to 6.5-7.5;
(f) and (d) freezing and drying the gauze treated in the step (d) to obtain the carboxymethyl cellulose potassium salt gauze.
The carboxymethyl cellulose potassium salt gauze prepared by the method is easier to be absorbed by organisms, can be used for stopping bleeding of internal wounds, and has no toxic or side effect.
As a preferred embodiment of the preparation method of the absorbable healing promoting hemostatic dressing of the present invention, in step (b), the alkalization treatment process is: soaking the pretreated absorbent cotton gauze in a KOH solution with the mass percentage of 10-20%, wherein the weight ratio of the absorbent cotton gauze to the KOH solution is as follows: absorbent cotton gauze: KOH solution ═ 1: and (4) performing an alkalization reaction by using acetone as a diluent, and reacting for 3-6 hours at the temperature of-20-37 ℃ under the condition of stirring or ultrasonic vibration to obtain the alkalized absorbent cotton gauze.
As a preferred embodiment of the preparation method of the absorbable healing-promoting hemostatic dressing, in the step (c), the weight ratio of the alkalized absorbent cotton gauze to the chloroacetic acid is: and (3) alkalizing the degreased cotton gauze: 1: 60-100 parts of chloroacetic acid, wherein the reaction conditions of etherification treatment are as follows: reacting for 2-3 h when the temperature rise rate is 1-2 ℃ and the temperature rises to 65 ℃, continuously raising the temperature to 85 ℃, and keeping the temperature constant for 1-2 h.
As a preferable embodiment of the preparation method of the absorbable healing-promoting hemostatic dressing, in the step (3), the cleaning is performed by using a methanol solution, and the drying is performed by adopting a freeze drying mode.
The invention has the beneficial effects that: the invention provides an absorbable healing-promoting hemostatic composition and a dressing. The absorbable healing-promoting hemostatic composition has better hemostatic and healing-promoting effects, is used together with a hemostatic material to improve the hemostatic speed, and particularly, the carboxymethyl cellulose sylvite gauze prepared by the method is easier to absorb by organisms and has no toxic or side reaction, and the preparation process of the absorbable healing-promoting hemostatic dressing is simple.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1
In one embodiment of the absorbable healing promoting hemostatic composition of the present invention, the absorbable healing promoting hemostatic composition of the present embodiment comprises the following components: 5 mu g of silver nitrate, 2.5g of vitamin K, 1g of vitamin E and 0.08g of epidermal growth factor.
An absorbable healing promoting hemostatic dressing comprising an absorbent substrate and the absorbable healing promoting hemostatic composition of the present embodiment; the absorbable heal-promoting hemostatic composition is attached to an adsorption substrate, the adsorption substrate is carboxymethyl cellulose potassium salt gauze, and the weight percentage of the absorbable heal-promoting hemostatic composition in the absorbable heal-promoting hemostatic dressing is 2%.
The preparation method of the absorbable wound dressing for promoting healing and hemostasis comprises the following steps:
(1) preparing an absorbable healing-promoting hemostatic composition solution: dissolving the absorbable healing promoting hemostatic composition in 30mL double distilled water;
(2) soaking carboxymethyl cellulose sylvite gauze in an absorbable healing-promoting hemostatic composition solution for 2 hours;
(3) taking out, repeatedly washing with methanol for many times, and freeze-drying to obtain the absorbable healing-promoting hemostatic dressing;
the preparation method of the carboxymethyl cellulose potassium salt gauze comprises the following steps:
(a) and (3) pretreatment: washing absorbent cotton gauze in double-distilled water at the temperature of 80 ℃ for 2h to obtain pretreated absorbent cotton gauze;
(b) and alkalization treatment: soaking the pretreated absorbent cotton gauze obtained in the step (a) in a KOH solution with the mass percentage of 18%, wherein the weight ratio of the absorbent cotton gauze to the KOH solution is as follows: absorbent cotton gauze: KOH solution ═ 1: 50, using acetone as a diluent to carry out an alkalization reaction, and reacting for 5 hours at the temperature of 25 ℃ under the conditions of stirring or ultrasonic vibration to obtain the alkalized absorbent cotton gauze;
(c) and (3) etherification treatment: adding chloroacetic acid into the alkalized absorbent cotton gauze obtained in the step (b) for etherification, wherein the weight ratio of the alkalized absorbent cotton gauze to the chloroacetic acid is as follows: and (3) alkalizing the degreased cotton gauze: 1:80 of chloroacetic acid, reacting for 2.5 hours at the temperature rising rate of 1.5 ℃ and the temperature rising temperature of 65 ℃, continuing to heat to 85 ℃, and keeping the temperature for 1.5 hours to obtain the etherified absorbent cotton gauze;
(d) alcohol washing and neutralization: washing the etherified absorbent cotton gauze obtained in the step (c) for multiple times by using methanol, and simultaneously dropwise adding glacial acetic acid to adjust the pH value to 6.5-7.5;
(f) and (d) freezing and drying the gauze treated in the step (d) to obtain carboxymethyl cellulose potassium salt gauze.
Example 2
In one embodiment of the absorbable healing promoting hemostatic composition of the present invention, the absorbable healing promoting hemostatic composition of the present embodiment comprises the following components: 3 mu g of silver nitrate, 1g of vitamin K, 0.5g of vitamin E and 0.065 part of epidermal growth factor.
An absorbable healing promoting hemostatic dressing comprising an absorbent substrate and the absorbable healing promoting hemostatic composition of the present embodiment; the absorbable heal-promoting hemostatic composition is attached to an adsorption substrate, the adsorption substrate is carboxymethyl cellulose potassium salt gauze, and the weight percentage of the absorbable heal-promoting hemostatic composition in the absorbable heal-promoting hemostatic dressing is 2%.
The preparation method of the absorbable wound dressing for promoting healing and hemostasis comprises the following steps:
(1) preparing an absorbable healing-promoting hemostatic composition solution: dissolving the absorbable healing promoting hemostatic composition in 30mL double distilled water;
(2) soaking carboxymethyl cellulose sylvite gauze in an absorbable healing-promoting hemostatic composition solution for 1 hour;
(3) taking out, repeatedly washing with methanol for many times, and freeze-drying to obtain the absorbable healing-promoting hemostatic dressing;
the preparation method of the carboxymethyl cellulose potassium salt gauze comprises the following steps:
(a) and (3) pretreatment: washing absorbent cotton gauze in double-distilled water at the temperature of 55 ℃ for 1h to obtain pretreated absorbent cotton gauze;
(b) and alkalization treatment: soaking the pretreated absorbent cotton gauze obtained in the step (a) in a KOH solution with the mass percentage of 10%, wherein the weight ratio of the absorbent cotton gauze to the KOH solution is as follows: absorbent cotton gauze: KOH solution ═ 1: 40, using acetone as a diluent to carry out an alkalization reaction, and reacting for 3 hours at the temperature of-20 ℃ under the stirring or ultrasonic vibration condition to obtain the alkalized absorbent cotton gauze;
(c) and (3) etherification treatment: adding chloroacetic acid into the alkalized absorbent cotton gauze obtained in the step (b) for etherification, wherein the weight ratio of the alkalized absorbent cotton gauze to the chloroacetic acid is as follows: and (3) alkalizing the degreased cotton gauze: 1:60 of chloroacetic acid, reacting for 2 hours at the temperature rising rate of 1 ℃ to 65 ℃, continuing to heat to 85 ℃, and keeping the temperature for 1 hour to obtain the etherified absorbent cotton gauze;
(d) alcohol washing and neutralization: washing the etherified absorbent cotton gauze obtained in the step (c) for multiple times by using methanol, and simultaneously dropwise adding glacial acetic acid to adjust the pH value to 6.5-7.5;
(f) and (d) freezing and drying the gauze treated in the step (d) to obtain carboxymethyl cellulose potassium salt gauze.
Example 3
In one embodiment of the absorbable healing promoting hemostatic composition of the present invention, the absorbable healing promoting hemostatic composition of the present embodiment comprises the following components: 7 mu g of silver nitrate, 4g of vitamin K, 2g of vitamin E and 0.1g of epidermal growth factor.
An absorbable healing promoting hemostatic dressing comprising an absorbent substrate and the absorbable healing promoting hemostatic composition of the present embodiment; the absorbable heal-promoting hemostatic composition is attached to an adsorption substrate, the adsorption substrate is carboxymethyl cellulose potassium salt gauze, and the weight percentage of the absorbable heal-promoting hemostatic composition in the absorbable heal-promoting hemostatic dressing is 2%.
The preparation method of the absorbable wound dressing for promoting healing and hemostasis comprises the following steps:
(1) preparing an absorbable healing-promoting hemostatic composition solution: dissolving the absorbable healing promoting hemostatic composition in 30mL double distilled water;
(2) soaking carboxymethyl cellulose sylvite gauze in an absorbable healing-promoting hemostatic composition solution for 3 hours;
(3) taking out, repeatedly washing with methanol for many times, and freeze-drying to obtain the absorbable healing-promoting hemostatic dressing;
the preparation method of the carboxymethyl cellulose potassium salt gauze comprises the following steps:
(a) and (3) pretreatment: washing absorbent cotton gauze in double-distilled water at the temperature of 85 ℃ for 4 hours to obtain pretreated absorbent cotton gauze;
(b) and alkalization treatment: soaking the pretreated absorbent cotton gauze obtained in the step (a) in a KOH solution with the mass percentage of 20%, wherein the weight ratio of the absorbent cotton gauze to the KOH solution is as follows: absorbent cotton gauze: KOH solution ═ 1: 55, using acetone as a diluent to carry out an alkalization reaction, and reacting for 6 hours at the temperature of 37 ℃ under the stirring or ultrasonic vibration condition to obtain the alkalized absorbent cotton gauze;
(c) and (3) etherification treatment: adding chloroacetic acid into the alkalized absorbent cotton gauze obtained in the step (b) for etherification, wherein the weight ratio of the alkalized absorbent cotton gauze to the chloroacetic acid is as follows: and (3) alkalizing the degreased cotton gauze: 1:100 of chloroacetic acid, reacting for 3 hours at the temperature rising rate of 2 ℃ and the temperature rising temperature of 65 ℃, continuing to heat to 85 ℃, and keeping the temperature for 2 hours to obtain the cotton wool gauze subjected to etherification treatment;
(d) alcohol washing and neutralization: washing the etherified absorbent cotton gauze obtained in the step (c) for multiple times by using methanol, and simultaneously dropwise adding glacial acetic acid to adjust the pH value to 6.5-7.5;
(f) and (d) freezing and drying the gauze treated in the step (d) to obtain carboxymethyl cellulose potassium salt gauze.
Example 4
In one embodiment of the absorbable healing promoting hemostatic composition of the present invention, the absorbable healing promoting hemostatic composition of the present embodiment comprises the following components: 4 mug of silver nitrate, 2g of vitamin K, 0.8g of vitamin E and 0.07g of epidermal growth factor.
An absorbable healing promoting hemostatic dressing comprising an absorbent substrate and the absorbable healing promoting hemostatic composition of the present embodiment; the absorbable heal-promoting hemostatic composition is attached to an adsorption substrate, the adsorption substrate is carboxymethyl cellulose potassium salt gauze, and the weight percentage of the absorbable heal-promoting hemostatic composition in the absorbable heal-promoting hemostatic dressing is 2%.
The absorbable wound dressing for promoting healing and hemostasis described in this example was prepared as described in example 1.
Example 5
In one embodiment of the absorbable healing promoting hemostatic composition of the present invention, the absorbable healing promoting hemostatic composition of the present embodiment comprises the following components: 6 mug of silver nitrate, 3g of vitamin K, 1.5g of vitamin E and 0.09g of epidermal growth factor.
An absorbable healing promoting hemostatic dressing comprising an absorbent substrate and the absorbable healing promoting hemostatic composition of the present embodiment; the absorbable heal-promoting hemostatic composition is attached to an adsorption substrate, the adsorption substrate is carboxymethyl cellulose potassium salt gauze, and the weight percentage of the absorbable heal-promoting hemostatic composition in the absorbable heal-promoting hemostatic dressing is 2%.
The absorbable wound dressing for promoting healing and hemostasis described in this example was prepared as described in example 1.
Example 6
The cytotoxicity detection of the absorbable healing-promoting hemostatic dressing of embodiments 1-5 comprises the following steps:
(1) preparing each gauze leaching liquor: leaching the absorbable healing-promoting hemostatic dressing and the instant yarn (American well-being) in the embodiments 1-5 according to 0.1g/mL after being saturated by a DMEM culture medium which is used for leaching and absorbing 10% fetal calf serum, and leaching for 24 hours at 37 ℃, wherein the leaching liquor is used as a test sample group;
(2) cell preparation: the frozen L929 cells were recovered and transferred to 2 generations for preparation of 1X 104Adding each/mL cell suspension into a 96-well plate, and culturing for 24h in a 5% carbon dioxide incubator at 37 ℃, wherein each well comprises 200 mu L of cell suspension;
(3) sample adding: discarding original culture solution from cultured cells, washing dead cells with PBS, adding 200 μ L of test sample into each well, and paralleling each sample;
(4) and (3) detection: and (3) placing the mixture in an incubator with 37 ℃ and 5% carbon dioxide, culturing for 24 hours, and detecting cytotoxicity by adopting an MTT method.
The test results are shown in table 1.
TABLE 1 cytotoxicity assay results for absorbable healing-promoting hemostatic dressings described in examples 1-5
As can be seen from the test results in Table 1, the absorbable accelerated healing hemostatic dressings and the instant yarns described in examples 1 to 5 are non-toxic, and the absorbable accelerated healing hemostatic dressings described in examples 1 to 5 can promote cell growth, wherein the effect of example 1 is the most significant.
Experimental example 7
The hemostatic time detection method of the absorbable healing-promoting hemostatic dressing comprises the following specific operation steps:
(1) selecting 12 healthy New Zealand white rabbits, disinfecting ear marginal veins, slowly injecting a 3% sodium pentobarbital solution, and carrying out an experiment after the rabbits are completely anesthetized;
(2) selecting the central aorta of the rabbit ears, disinfecting the artery of the rabbit ears by iodophor, and then using a scalpel as a wound surface of 1 multiplied by 1 cm;
(3) after the ear artery of the rabbit bleeds, blood is absorbed by using common sterile gauze, and the absorbable healing-promoting hemostatic dressing prepared in the embodiment 1-5 is cut into 2 x 2cm and applied to the bleeding wound surface;
(4) absorbing exuded blood of the wound surface by using common sterile gauze every 20s, and observing the hemostatic effect;
(5) three parallel experiments were performed with each dressing using speed-instant yarn (american hadamard) as a control, and the time to complete hemostasis was recorded separately and the average calculated.
The test results are shown in table 2.
Table 2 results of testing hemostatic time of absorbable healing-promoting hemostatic dressings described in examples 1 to 5
As can be seen from the results in Table 2, the hemostatic time of the absorbable accelerated healing hemostatic dressings of examples 1-5 is less than that of the immediate healing hemostatic dressings and significantly different (P <0.05), and the hemostatic time of example 1 is the shortest.
Experimental example 8
The method for detecting the degradation speed of the absorbable healing-promoting hemostatic dressing comprises the following specific operation steps:
(1) selecting 12 healthy New Zealand white rabbits, disinfecting ear marginal veins, slowly injecting a 3% sodium pentobarbital solution, and carrying out an experiment after the rabbits are completely anesthetized;
(2) selecting the central aorta of the ear of the rabbit, shaving and disinfecting the thigh part of the rabbit by iodophor, and then using a scalpel as a wound surface of 1 cm;
(3) after bleeding, absorbing blood by using common sterile gauze, cutting the absorbable healing-promoting hemostatic dressing of the embodiment 1-5 into 1 x 2cm strips, placing the strips in the wound, and suturing the wound;
(4) observing the change of the abdominal wound every day, removing stitches after 8 days, and observing the degradation condition of each gauze.
(5) Three parallel experiments were performed for each dressing using speed, i.e. gauze (hadamard usa) as a control, and the remaining amount of each dressing was recorded separately and the average was calculated.
The test results are shown in table 3.
TABLE 3 degradation Rate test results for the absorbable healing-promoting hemostatic dressings of examples 1-5
| Sample set | Example 1 | Example 2 | Example 3 | Example 4 | Example 5 | Instant yarn |
| The remaining amount of each gauze (%) | 0 | 3 | 5 | 1 | 2 | 10 |
The results in table 3 show that the absorbable healing-promoting hemostatic dressings in examples 1 to 5 have a degradation rate greater than the speed, i.e., the difference between the degradation rates is significant (P <0.05), and the degradation rate of example 1 is the fastest.
Example 9
In order to explore the proportion of the absorbable healing promoting and hemostatic composition and the effects of promoting wound healing and hemostasis, 17 test groups and 4 control groups in table 4 are arranged, the absorbable healing promoting and hemostatic compositions of the test groups 1-17 and the control groups 1-4 are prepared into the absorbable healing promoting and hemostatic dressing according to the preparation method of the absorbable healing promoting and hemostatic dressing described in embodiment 1, the sum of the total amounts of the absorbable healing promoting and hemostatic compositions of the test groups 1-17 and the control groups 1-4 is the same as that of embodiment 1, the effects of promoting wound healing and hemostasis of the test groups 1-17 and the control groups 1-4 are tested, the method for testing the effect of promoting wound healing is the same as that of embodiment 6, and the method for testing the hemostatic effect is the same as that of embodiment 7.
TABLE 4 absorbable healing-promoting hemostatic compositions of different compositions
The test results are shown in Table 5.
TABLE 5 results of cell growth promotion and hemostasis time tests of absorbable accelerated healing hemostatic compositions of various compositions
From the test results in table 5, it can be seen that when the absorbable composition for promoting healing and stopping bleeding adopts the mixture ratio in test group 1, the cell growth rate and the stopping bleeding rate are the best, and the cell growth rate and the stopping bleeding rate of test groups 3, 4, 7, 8, 11, 12, 15, 16 are superior to those of test groups 2, 5, 6, 9, 10, 14, 17, and are all superior to those of controls 1-4, which indicates that the absorbable composition for promoting healing and stopping bleeding can better exert the effects of promoting healing and stopping bleeding and resisting bacteria only under a certain mixture ratio.
Example 10
In order to explore the effect of the content of the absorbable healing promoting and hemostatic composition in the dressing on promoting wound healing and hemostasis, 5 test groups and 2 control groups in table 6 are set, the absorbable healing promoting and hemostatic compositions of the test groups 1-5 and the control groups 1 and 2 are prepared into the absorbable healing promoting and hemostatic dressing according to the composition and the preparation method of the embodiment 1, the effect of promoting wound healing and hemostasis of the test groups 1-5 and the control groups 1 and 2 is tested, the test method of the effect of promoting wound healing is the same as that of the embodiment 6, and the test method of the hemostatic effect is the same as that of the embodiment 7.
TABLE 6 test results of different contents of the absorbable accelerated healing hemostatic composition for promoting cell growth and hemostasis time
From the results in table 6, it can be seen that the absorbable pro-healing hemostatic composition is effective in shortening the hemostatic time and promoting wound healing when the content of the absorbable pro-healing hemostatic composition in the dressing is 0.1% by weight, and the hemostatic speed and the healing promoting effect are increased with the increase of the added amount of the absorbable pro-healing hemostatic composition, but the content of the absorbable pro-healing hemostatic composition is increased more than 2% in the dressing, and the improvement of the hemostatic speed and the wound healing promoting effect is not significant.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (1)
1. An absorbable healing promoting hemostatic dressing, comprising an absorbent substrate and an absorbable healing promoting hemostatic composition; the absorbable healing promoting hemostatic composition is attached to an absorbent substrate;
the absorbable healing promoting hemostatic composition comprises the following components in parts by weight: silver nitrate 5X 10-6Parts of vitamin K2.5 parts, vitamin E1 part and epidermal growth factor 0.08 part;
the absorbable healing promoting hemostatic composition accounts for 2 percent by weight of the absorbable healing promoting hemostatic dressing; the adsorption base material is carboxymethyl cellulose sylvite gauze;
the preparation method of the absorbable wound dressing capable of promoting healing and hemostasis comprises the following steps:
(1) preparing an absorbable healing-promoting hemostatic composition solution: adding the absorbable healing promoting hemostatic composition into double distilled water;
(2) soaking the adsorption substrate in the absorbable healing-promoting and hemostatic composition solution for 1-3 hours;
(3) taking out, cleaning and drying to obtain the absorbable healing-promoting hemostatic dressing;
the preparation method of the carboxymethyl cellulose potassium salt gauze comprises the following steps:
(a) and (3) pretreatment: washing absorbent cotton gauze in double-distilled water at the temperature of 55-85 ℃ for 1-4 h to obtain pretreated absorbent cotton gauze;
(b) and alkalization treatment: performing alkalization treatment on the pretreated absorbent cotton gauze obtained in the step (a) to obtain alkalized absorbent cotton gauze;
(c) and (3) etherification treatment: adding chloroacetic acid into the alkalized absorbent cotton gauze obtained in the step (b) for etherification to obtain the etherified absorbent cotton gauze;
(d) alcohol washing and neutralization: washing the etherified absorbent cotton gauze obtained in the step (c) for multiple times by using methanol, and simultaneously dropwise adding glacial acetic acid to adjust the pH value to 6.5-7.5;
(f) freezing and drying the gauze treated in the step (d) to obtain carboxymethyl cellulose sylvite gauze;
in the step (b), the alkalization process comprises the following steps: soaking the pretreated absorbent cotton gauze in a KOH solution with the mass percentage of 10-20%, wherein the weight ratio of the absorbent cotton gauze to the KOH solution is as follows: absorbent cotton gauze: KOH solution =1: 40-55, using acetone as a diluent to carry out an alkalization reaction, and reacting for 3-6 hours at the temperature of-20-37 ℃ under the stirring or ultrasonic vibration condition to obtain the alkalized absorbent cotton gauze;
in the step (c), the weight ratio of the degreased cotton gauze subjected to the alkalization treatment to the chloroacetic acid is as follows: and (3) alkalizing the degreased cotton gauze: chloroacetic acid =1: 60-100, and the reaction conditions of the etherification treatment are as follows: reacting for 2-3 h when the temperature rise rate is 1-2 ℃ and the temperature rises to 65 ℃, continuously raising the temperature to 85 ℃, and keeping the temperature constant for 1-2 h.
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| CN101316618A (en) * | 2005-11-14 | 2008-12-03 | 株式会社大熊 | Sustained release film formulation for healing wound comprising epidermal growth factor |
| CN102526032A (en) * | 2011-12-27 | 2012-07-04 | 贵州扬生医用器材有限公司 | Formula of liposome preparation containing compound amino acid and preparation method thereof |
| CN106474525A (en) * | 2016-11-16 | 2017-03-08 | 广东泰宝医疗科技股份有限公司 | A kind of novel antibacterial hemostatic gauze and preparation method thereof |
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| DE19925519A1 (en) * | 1999-06-04 | 2000-12-07 | Lohmann Therapie Syst Lts | Laminar wound dressing for controlled release of active substance e.g. platelet derived growth factor, includes layer containing polymer and hydrogel as fluid absorber |
| GB2362100B (en) * | 2000-05-08 | 2002-05-08 | Maelor Pharmaceuticals Ltd | Wound gels |
| WO2012112797A2 (en) * | 2011-02-16 | 2012-08-23 | Barofsky Andrew | Wound stasis dressing for large surface wounds |
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| CN101316618A (en) * | 2005-11-14 | 2008-12-03 | 株式会社大熊 | Sustained release film formulation for healing wound comprising epidermal growth factor |
| CN102526032A (en) * | 2011-12-27 | 2012-07-04 | 贵州扬生医用器材有限公司 | Formula of liposome preparation containing compound amino acid and preparation method thereof |
| CN106474525A (en) * | 2016-11-16 | 2017-03-08 | 广东泰宝医疗科技股份有限公司 | A kind of novel antibacterial hemostatic gauze and preparation method thereof |
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