CN106421868A - Chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material and preparation method thereof - Google Patents
Chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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Abstract
The invention belongs to the technical field of biomedical engineering, and particularly relates to a chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material and a preparation method thereof. The preparation method of the chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material specifically comprises the following steps: S1, preparing a chitosan quaternary ammonium salt; S2, processing a porcine acellular dermal matrix; and S3, preparing the chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material. The chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material disclosed by the invention completely reserves morphological structures and components of cells, has the characteristic of low antigenicity, and meanwhile can induce fiber-forming cells and vascular endothelial cells with regeneration capacity to grow therein.
Description
Technical field
The invention belongs to biomedical engineering field is and in particular to a kind of chitosan quaternary ammonium salt acellular porcine dermal matrix applies
Material and preparation method thereof.
Background technology
Skin is the barrier maintaining homeostasis and stoping microorganism invasion.The final goal of skin wound treatment is weight
Build or recover skin barrier, a Wound dressing of good performance temporarily can play barrier action, provide a favourable surface of a wound to heal
The environment closing.Preferably Wound dressing should have:1. good adhesiveness, permeability, has the moisture of similar normal skin again
Evaporation rate;2. bacterial invasion and restricting bacterial can be stoped to colonize on the surface of a wound;3. mitigate pain;4. there is good elasticity and soft
Toughness, can be suitable for human body different parts with bodily form flap coverage;5. the paralysed trace causing after effectively preventing from transplanting is formed and the surface of a wound
Shrink;6. can induced cell growth, promote autologous fibroblasts to grow in xenogenesis support;Wound healing, outer to reach
See smooth purpose;7. to tissue nonirritant, nontoxicity, no antigen, there is good biocompatibility;8. often it is resistant to
Processed with sterilization method, can preserve long-term, and can produce in a large number.
Acellular dermal matrix eliminates the epidermal area in skin and the cell containing antigenic component only retains in corium containing glue
The extracellular matrix of former rack, has opposing originality, good biology performance and mechanical property, and being that one kind is preferably biological applies
Material.Xenogenesis (pig) acellular dermal matrix has wide material sources compared to acellular dermal matrix in implant therapy, the advantages of of high quality and at a reasonable price,
And very high tissue homology and highly similar is had to human body skin on collagen content, structure, clinically takes
Obtain and extensively apply.But acellular porcine dermal matrix does not have antibacterial ability, led to by bacterium infection
Trauma surface infestation, has the shortcomings that the danger of cross-infection again, limits its application clinically as dressing.
Content of the invention
Pin of the present invention solves acellular porcine dermal matrix as the shortcoming being easily subject to bacterium infection during dressing, provides a kind of shell
Glycan quaternary ammonium salt acellular porcine dermal matrix dressing and preparation method thereof.
For achieving the above object, technical scheme is as follows：A kind of chitosan quaternary ammonium salt acellular porcine dermal matrix
Dressing, is made up of with chitosan quaternary ammonium salt acellular porcine dermal matrix, and described chitosan quaternary ammonium salt is shitosan via shrink
Glycerine TEBA or glycidyltrimetiiylammonium ammonium chloride are obtained.
A kind of preparation method of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing, described preparation method concrete steps are such as
Under：
S1, the preparation of chitosan quaternary ammonium salt：（a）, shitosan is dissolved in glacial acetic acid solution, make chitosan solution, will be described
Add NaOH solution in chitosan solution, so that pH is adjusted to more than 9, shitosan floccule is separated out；（b）, by described shitosan
Floccule carries out centrifugal treating with the speed of 3000rpm after adding distilled water stirring and obtains precipitation in 10 minutes, then will with distilled water
Described precipitation is washed till neutrality；（c）, by step（b）Described precipitation is placed in round-bottomed flask, adds isopropanol, is heated to 75-80 DEG C
Reaction 30-60min, is subsequently adding glycidol TEBA or the aqueous solution of glycidyltrimetiiylammonium ammonium chloride,
75-80 DEG C of reaction 6h, rotary evaporation removes excess of solvent, adds ethanol/acetone mixed liquor, stands 10-30min, filters, obtain shell
Glycan quaternary ammonium salt；（d）, with distilled water by chitosan quaternary ammonium salt prepare formed mass concentration be 5% chitosan quaternary ammonium salting liquid with
Standby；
S2, the process of acellular porcine dermal matrix：Acellular porcine dermal matrix is placed in ethanol and carries out processed, Ran Houzai
It is placed in freeze-drying in -80 DEG C of freeze driers, last ultraviolet irradiation sterilizing 3-6h；
S3, the preparation of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing:By step（S2）The de- cell of pig processing gained is true
Scytoblastema matter, is soaked in step（S1）In described chitosan quaternary ammonium salting liquid, ultrasonically treated 1-2h while stirring at room temperature, stirring
Speed is 1500-2000rpm, then in an aseptic environment, acellular porcine dermal matrix is taken out, is then rushed with absolute ethyl alcohol
Wash, be placed in freeze-drying in -80 DEG C of freeze driers, obtain final product chitosan quaternary ammonium salt acellular porcine dermal matrix dressing.
Preferably, step（S1）Described in glacial acetic acid concentration be 1%（Mass concentration）, the concentration of NaOH solution is
1mol/L, the amount of addition isopropanol is 3-5 times of the volume of shitosan floccule, and ethanol/acetone mixed solution is by ethanol and third
Ketone by volume 1:1 mixes.
Preferably, step（S1）Described in glycidol TEBA or glycidyltrimetiiylammonium ammonium chloride
Addition and the ratio of addition of shitosan be by glycidol TEBA or glycidyltrimetiiylammonium chlorine
Change the amount of contained epoxide group material in ammonium（N1）Amino content with shitosan（N2）Ratio between two is 1:1 or 1:2.
Preferably, step（2）Described in the dehydration process steps of acellular porcine dermal matrix be：First pig is taken off cell true
Scytoblastema matter is soaked in each 6h in 75%, 85%, 95% ethanol successively, is subsequently placed in ultrasonically treated 60min in absolute ethyl alcohol.
Preferably, step（3）Described in acellular porcine dermal matrix to be soaked in amount in chitosan quaternary ammonium salting liquid be shell
Glycan quaternary ammonium salt solution is 50 with the volume ratio of acellular porcine dermal matrix:1.
Beneficial effects of the present invention are：
（1）The chitosan quaternary ammonium salt acellular porcine dermal matrix dressing of the present invention intactly remains morphosis and the group of cell
Become composition, have the characteristics that antigenicity is low, can induce the fibroblast with power of regeneration and vascular endothelial cell at it simultaneously
Middle growth.
（2）The chitosan quaternary ammonium salt being compounded on acellular porcine dermal matrix has slow release effect, can continuingly act on
Regenerated bacteria, can reach the lasting, effect of high-efficiency antimicrobial, can reduce the dressing change frequency of dressing, reduce the risk of cross-infection, with
When decrease the misery brought during dressing for patient.
（3）Chitosan quaternary ammonium salt will not produce drug resistance compared to traditional antiseptic, has lasting anti-infection ability
With good biocompatibility, and chitosan quaternary ammonium salt has the effect of wound healing.
（4）By carrying out multiple serial dehydration, ultrasonic, freeze-drying process to acellular porcine dermal matrix, pig is made to take off
In acellular dermal matrix, the immunogenic water-soluble substances that may cause of residual removes, and makes acellular dermal matrix original
Tridimensional network more loose so that chitosan quaternary ammonium salt can preferably be compound to wherein.
（5）Obtained chitosan quaternary ammonium salt acellular porcine dermal matrix dressing in the present invention, due to chitosan quaternary ammonium salt
There is good dissolubility, after being moistened with SPSS when using, hydrogel, dressing energy can be formed on the surface of dressing
Preferably stick on the surface of a wound, reduce the water loss of the surface of a wound, alleviate pain.Further, since chitosan quaternary ammonium salt has well
Absorbency and there is certain hemostatic function, the chitosan quaternary ammonium salt acellular porcine dermal matrix dressing energy obtained by the present invention
Play haemostatic effect.
（6）Compared to the other antiseptics of interpolation（As Nano Silver, antibiotic etc.）, the chitosan quaternary ammonium salt that the present invention adopts
There is good biological safety and long-acting anti-microbial property.
Specific embodiment
With reference to embodiment, technical scheme is described further.
Embodiment 1
A kind of preparation method of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing, described preparation method comprises the following steps that：
S1, the preparation of chitosan quaternary ammonium salt：By a certain amount of shitosan（Amino content is n1）It is dissolved in the ice that mass concentration is 1%
In acetic acid solution, make chitosan solution, then adjust its pH to more than 9 with the NaOH solution of 1mol/L, make shitosan floccule
Separate out, after adding distilled water stirring 1h, 3000rpm is centrifuged 10 min, obtains shitosan precipitation, is subsequently adding distilled water submergence and sinks
Form sediment stirring 1h, the pH value of test water, then with distilled water, precipitation is washed till neutrality.Shitosan precipitation is placed in round-bottomed flask, plus
Enter isopropanol（By isopropanol and shitosan precipitation volume than for 5：1）, it is heated to 80 DEG C, react 60min, then take containing epoxy radicals
The amount of group's material is n2（n1:n2=1:1）Glycidol TEBA be dissolved in distilled water, be added dropwise in flask
The aqueous solution, 80 DEG C of reaction 6h, rotary evaporation removes excess of solvent, is subsequently adding the ethanol/acetone mixed liquor of 10 times of volumes（Ethanol
Volume ratio with acetone is 1:1）, stand 30min, suction filtration, it is placed in drying in 60 DEG C of vacuum drying chambers to leach sediment, that is,
Obtain chitosan quaternary ammonium salt, finally with distilled water, chitosan quaternary ammonium salt is prepared the chitosan quaternary ammonium salt that formation mass concentration is 5% molten
Liquid is with standby；
S2, acellular porcine dermal matrix is soaked in 6h in 75% ethanol；It is placed in 6h in 85% ethanol after taking-up；It is placed in after taking-up again
6h in 95% ethanol, is then sonicated 10min, takes out under sterile working from soak, and ultraviolet irradiation 3h sterilizes；
S3, the preparation of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing:The pig processing gained in step 2 is taken off cell true
Scytoblastema matter is soaked in ultrasonically treated 2h in the chitosan quaternary ammonium salting liquid that mass concentration is 5%, is repeated 3 times, shitosan during being somebody's turn to do
Quaternary ammonium salt solution is 50 with the volume ratio of acellular porcine dermal matrix:1, after the completion of at 4 DEG C low temperature place 24h, take out soak
Acellular porcine dermal matrix afterwards, after being rinsed several times with PBS cushioning liquid, freeze-drying obtains final product chitosan quaternary ammonium salt pig and takes off cell
Dermal matrix dressing.
Embodiment 2
A kind of preparation method of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing, described preparation method comprises the following steps that：
S1, the preparation of chitosan quaternary ammonium salt：By a certain amount of shitosan（Amino content is n1）It is dissolved in the ice that mass concentration is 1%
In acetic acid solution, make chitosan solution, then adjust its pH with the NaOH solution of 1mol/L and be more than 9, so that shitosan floccule is analysed
Go out, after adding distilled water stirring 1h, 3000rpm is centrifuged 10 min, obtains shitosan precipitation, is subsequently adding distilled water immersion precipitation
Stirring 1h, the pH value of test water, then with distilled water, precipitation is washed till neutrality.Shitosan precipitation is placed in round-bottomed flask, adds
Isopropanol（By isopropanol and shitosan precipitation volume than for 5：1）, it is heated to 75 DEG C, react 30min, then take containing epoxide group
The amount of material is n2（n1:n2=1:2）Glycidyltrimetiiylammonium ammonium chloride be dissolved in distilled water, be added dropwise over water in flask
Solution, 75 DEG C of reaction 6h, rotary evaporation removes excess of solvent, is subsequently adding the ethanol/acetone mixed liquor of 10 times of volumes（Ethanol with
The volume ratio of acetone is 1:1）, stand 30min, suction filtration, it is placed in drying in 60 DEG C of vacuum drying chambers to leach sediment, obtains final product
Chitosan quaternary ammonium salt is finally prepared with distilled water and is formed the chitosan quaternary ammonium salting liquid that mass concentration is 5% by chitosan quaternary ammonium salt
With standby；
S2, acellular porcine dermal matrix is soaked in 12h in 75% ethanol；It is placed in 12h in 85% ethanol after taking-up；Put again after taking-up
12h in 95% ethanol, is then sonicated 10min, takes out under sterile working from soak, and ultraviolet irradiation 3h sterilizes；
S3, the preparation of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing:The pig acellular dermal of gained will be processed in step 2
Matrix is soaked in ultrasonically treated 2h in the chitosan quaternary ammonium salting liquid that mass concentration is 5%, is repeated 3 times, shitosan season during being somebody's turn to do
Ammonium salt solution is 50 with the volume ratio of acellular porcine dermal matrix:1, after the completion of at 4 DEG C low temperature place 24h, take out soak after
Acellular porcine dermal matrix, with PBS cushioning liquid rinse several times after, freeze-drying obtain final product chitosan quaternary ammonium salt pig take off cell true
Scytoblastema matter dressing.
Embodiment 3：Animal wound healing assay
Take Male New Zealand rabbits, 40, every 2 ~ 3kg, it is randomly divided into four groups, every group 10.Each rabbit is all according to clinical animal
Experimental burning master pattern preparation method all causes the deep II degree wound of surgery leaves, and wherein A group rabbit is first stitched to wound
Close and then wrapped up using clean gauze and changed a gauze every three days, B group rabbit first carries out suturing then to wound
Bound up a wound for dressing using acellular porcine dermal matrix, the chitosan quaternary ammonium salt of C, D group difference application embodiment of the present invention 1,2
Acellular porcine dermal matrix dressing, and process of the test need not more change dressings, until the wound healing of C, D group just terminates this animal
Experiment.
Table 1 each group animal trauma surface infestation and healing state (%)
Knowable to above-mentioned test, simply use clean gauze and bound up a wound, wound infection rate is high, healing rate is slow, and more
Obvious scar is left after conjunction；
And bound up a wound for dressing using acellular porcine dermal matrix and produce with reducing scar although improve wound healing time
Raw, but due in agglutination wound easily occur infecting, lead to healing rate slow；
Two groups of C, D of the chitosan quaternary ammonium salt acellular porcine dermal matrix dressing of the application embodiment of the present invention 1,2 does not go out respectively
Existing red and swollen, fester phenomenon, speed of wound healing is fast, and after wound healing, no scar produces, and illustrates that the present invention possesses to have and continues
Antibacterial ability, and energy induced cell growth, improve speed of wound healing, effectively reduce scar and produce.
It should be pointed out that the specific embodiment more representational example that is the present invention is it is clear that the skill of the present invention
Art scheme is not limited to above-described embodiment, can also have many variations.Those of ordinary skill in the art, clearly public with present invention institute
What what written description that open or according to file was undoubted obtained, all it is considered as this patent scope of the claimed.
Claims (6)
1. a kind of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing is it is characterised in that by acellular porcine dermal matrix and shell
Glycan quaternary ammonium salt forms, and described chitosan quaternary ammonium salt is shitosan via glycidol TEBA or glycidol
Base trimethyl ammonium chloride is modified to be obtained.
2. the preparation method of a kind of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing according to claim 1, it is special
Levy and be, described preparation method comprises the following steps that：
S1, the preparation of chitosan quaternary ammonium salt：（a）, shitosan is dissolved in glacial acetic acid solution, make chitosan solution, will be described
Add NaOH solution in chitosan solution, so that pH is adjusted to more than 9, shitosan floccule is separated out；（b）, by described shitosan
Floccule carries out centrifugal treating with the speed of 3000rpm after adding distilled water stirring and obtains precipitation in 10 minutes, then will with distilled water
Described precipitation is washed till neutrality；（c）, by step（b）Described precipitation is placed in round-bottomed flask, adds isopropanol, is heated to 75-80 DEG C
Reaction 30-60min, is subsequently adding glycidol TEBA or the aqueous solution of glycidyltrimetiiylammonium ammonium chloride,
75-80 DEG C of reaction 6h, rotary evaporation removes excess of solvent, adds ethanol/acetone mixed liquor, stands 10-30min, filters, obtain shell
Glycan quaternary ammonium salt；（d）, with distilled water by chitosan quaternary ammonium salt prepare formed mass concentration be 5% chitosan quaternary ammonium salting liquid with
Standby；
S2, the process of acellular porcine dermal matrix：Acellular porcine dermal matrix is placed in ethanol and carries out processed, Ran Houzai
It is placed in freeze-drying in -80 DEG C of freeze driers, last ultraviolet irradiation sterilizing 3-6h；
S3, the preparation of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing:By step（S2）The de- cell of pig processing gained is true
Scytoblastema matter, is soaked in step（S1）In described chitosan quaternary ammonium salting liquid, ultrasonically treated 1-2h while stirring at room temperature, stirring
Speed is 1500-2000rpm, then in an aseptic environment, acellular porcine dermal matrix is taken out, is then rushed with absolute ethyl alcohol
Wash, be placed in freeze-drying in -80 DEG C of freeze driers, obtain final product chitosan quaternary ammonium salt acellular porcine dermal matrix dressing.
3. the preparation method of a kind of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing according to claim 2, it is special
Levy and be：Step（S1）Described in glacial acetic acid concentration be 1%（Mass concentration）, the concentration of NaOH solution is 1mol/L, adds
The amount of isopropanol is 3-5 times of volume of shitosan floccule, and ethanol/acetone mixed solution is by ethanol and acetone by volume 1:
1 mixes.
4. the preparation method of a kind of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing according to claim 2, it is special
Levy and be, step（S1）Described in glycidol TEBA or glycidyltrimetiiylammonium ammonium chloride addition
Ratio with the addition of shitosan is by institute in glycidol TEBA or glycidyltrimetiiylammonium ammonium chloride
The amount of the epoxide group material containing（N1）Amino content with shitosan（N2）Ratio between two is 1:1 or 1:2.
5. the preparation method of a kind of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing according to claim 2, it is special
Levy and be：Step（2）Described in the dehydration process steps of acellular porcine dermal matrix be：First by acellular porcine dermal matrix according to
Secondary be soaked in each 6h in 75%, 85%, 95% ethanol, be subsequently placed in ultrasonically treated 60min in absolute ethyl alcohol.
6. the preparation method of a kind of chitosan quaternary ammonium salt acellular porcine dermal matrix dressing according to claim 2, it is special
Levy and be：Step（3）Described in acellular porcine dermal matrix to be soaked in amount in chitosan quaternary ammonium salting liquid be chitosan quaternary ammonium
Salting liquid is 50 with the volume ratio of acellular porcine dermal matrix:1.
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| CN109847088A (en) * | 2019-01-18 | 2019-06-07 | 广州润虹医药科技股份有限公司 | Compound acellular dermal matrix biological dressing and preparation method thereof |
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| CN109078223A (en) * | 2018-09-05 | 2018-12-25 | 安徽徽科生物工程技术有限公司 | Bionical wound repairing membrane and preparation method thereof |
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| CN109847088A (en) * | 2019-01-18 | 2019-06-07 | 广州润虹医药科技股份有限公司 | Compound acellular dermal matrix biological dressing and preparation method thereof |
| CN110623792A (en) * | 2019-09-23 | 2019-12-31 | 温州医科大学附属第一医院 | Medical dressing and preparation method thereof |
| CN110623792B (en) * | 2019-09-23 | 2022-05-06 | 温州医科大学附属第一医院 | Medical dressing and preparation method thereof |
| CN110960729A (en) * | 2019-12-10 | 2020-04-07 | 山东大学 | Polysaccharide modified acellular matrix composite material, preparation and application |
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| CN111407919B (en) * | 2020-03-18 | 2022-05-13 | 天津市康婷生物工程集团有限公司 | Chitosan quaternary ammonium salt sterilization gauze and preparation method thereof |
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