CN103443289A - 液滴中的碎片化的基因组dna的分析 - Google Patents

液滴中的碎片化的基因组dna的分析 Download PDF

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Publication number
CN103443289A
CN103443289A CN2011800528318A CN201180052831A CN103443289A CN 103443289 A CN103443289 A CN 103443289A CN 2011800528318 A CN2011800528318 A CN 2011800528318A CN 201180052831 A CN201180052831 A CN 201180052831A CN 103443289 A CN103443289 A CN 103443289A
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CN
China
Prior art keywords
drop
dna
fragmentation
genomic dna
approximately
Prior art date
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Pending
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CN2011800528318A
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English (en)
Chinese (zh)
Inventor
凯文·D·奈斯
艾米·L·海德森
保罗·W·怀亚特
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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Priority claimed from US12/976,827 external-priority patent/US9598725B2/en
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CN103443289A publication Critical patent/CN103443289A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2011800528318A 2010-11-01 2011-11-01 液滴中的碎片化的基因组dna的分析 Pending CN103443289A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US40910610P 2010-11-01 2010-11-01
US61/409,106 2010-11-01
US12/976,827 2010-12-22
US12/976,827 US9598725B2 (en) 2010-03-02 2010-12-22 Emulsion chemistry for encapsulated droplets
PCT/US2011/058854 WO2012061442A1 (fr) 2010-11-01 2011-11-01 Analyse d'adn génomique fragmenté dans des gouttelettes

Publications (1)

Publication Number Publication Date
CN103443289A true CN103443289A (zh) 2013-12-11

Family

ID=46024812

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011800528318A Pending CN103443289A (zh) 2010-11-01 2011-11-01 液滴中的碎片化的基因组dna的分析

Country Status (7)

Country Link
EP (1) EP2635708A4 (fr)
JP (1) JP2013541347A (fr)
CN (1) CN103443289A (fr)
AU (1) AU2011323507A1 (fr)
CA (1) CA2816702A1 (fr)
SG (1) SG190076A1 (fr)
WO (1) WO2012061442A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10760116B2 (en) 2015-08-28 2020-09-01 Kabushiki Kaisha Dnaform Analysis kit, analyzer, and methods for analyzing template nucleic acid or target substance
WO2018098438A1 (fr) * 2016-11-28 2018-05-31 Arizona Board Of Regents On Behalf Of Arizona State University Systèmes et procédés liés à une réaction de gouttelettes à écoulement continu

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5555191A (en) * 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
US20050277125A1 (en) * 2003-10-27 2005-12-15 Massachusetts Institute Of Technology High-density reaction chambers and methods of use
US20100069263A1 (en) * 2008-09-12 2010-03-18 Washington, University Of Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20100137163A1 (en) * 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5555191A (en) * 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
US20050277125A1 (en) * 2003-10-27 2005-12-15 Massachusetts Institute Of Technology High-density reaction chambers and methods of use
US20100137163A1 (en) * 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
US20100069263A1 (en) * 2008-09-12 2010-03-18 Washington, University Of Sequence tag directed subassembly of short sequencing reads into long sequencing reads

Also Published As

Publication number Publication date
JP2013541347A (ja) 2013-11-14
AU2011323507A1 (en) 2013-06-13
WO2012061442A1 (fr) 2012-05-10
SG190076A1 (en) 2013-06-28
EP2635708A4 (fr) 2014-10-29
CA2816702A1 (fr) 2012-05-10
EP2635708A1 (fr) 2013-09-11

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Application publication date: 20131211