CA2816702A1 - Analyse d'adn genomique fragmente dans des gouttelettes - Google Patents

Analyse d'adn genomique fragmente dans des gouttelettes Download PDF

Info

Publication number
CA2816702A1
CA2816702A1 CA2816702A CA2816702A CA2816702A1 CA 2816702 A1 CA2816702 A1 CA 2816702A1 CA 2816702 A CA2816702 A CA 2816702A CA 2816702 A CA2816702 A CA 2816702A CA 2816702 A1 CA2816702 A1 CA 2816702A1
Authority
CA
Canada
Prior art keywords
droplets
dna
genomic dna
per
droplet
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2816702A
Other languages
English (en)
Inventor
Amy L. Hiddessen
Kevin D. Ness
Paul W. Wyatt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/976,827 external-priority patent/US9598725B2/en
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CA2816702A1 publication Critical patent/CA2816702A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2816702A 2010-11-01 2011-11-01 Analyse d'adn genomique fragmente dans des gouttelettes Abandoned CA2816702A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US40910610P 2010-11-01 2010-11-01
US61/409,106 2010-11-01
US12/976,827 2010-12-22
US12/976,827 US9598725B2 (en) 2010-03-02 2010-12-22 Emulsion chemistry for encapsulated droplets
PCT/US2011/058854 WO2012061442A1 (fr) 2010-11-01 2011-11-01 Analyse d'adn génomique fragmenté dans des gouttelettes

Publications (1)

Publication Number Publication Date
CA2816702A1 true CA2816702A1 (fr) 2012-05-10

Family

ID=46024812

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2816702A Abandoned CA2816702A1 (fr) 2010-11-01 2011-11-01 Analyse d'adn genomique fragmente dans des gouttelettes

Country Status (7)

Country Link
EP (1) EP2635708A4 (fr)
JP (1) JP2013541347A (fr)
CN (1) CN103443289A (fr)
AU (1) AU2011323507A1 (fr)
CA (1) CA2816702A1 (fr)
SG (1) SG190076A1 (fr)
WO (1) WO2012061442A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2017038682A1 (ja) 2015-08-28 2018-06-14 国立研究開発法人理化学研究所 鋳型核酸の分析方法、標的物質の分析方法、鋳型核酸または標的物質の分析用キット、および鋳型核酸または標的物質の分析用装置
EP3544737A1 (fr) * 2016-11-28 2019-10-02 Arizona Board of Regents on behalf of Arizona State University Systèmes et procédés liés à une réaction de gouttelettes à écoulement continu

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5555191A (en) * 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
WO2005043154A2 (fr) * 2003-10-27 2005-05-12 Massachusetts Institute Of Technology Chambres de réaction haute densité et procédés d'utilisation
JP2009536313A (ja) * 2006-01-11 2009-10-08 レインダンス テクノロジーズ， インコーポレイテッド ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法
US8383345B2 (en) * 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system

Also Published As

Publication number Publication date
CN103443289A (zh) 2013-12-11
SG190076A1 (en) 2013-06-28
AU2011323507A1 (en) 2013-06-13
WO2012061442A1 (fr) 2012-05-10
EP2635708A4 (fr) 2014-10-29
JP2013541347A (ja) 2013-11-14
EP2635708A1 (fr) 2013-09-11

Similar Documents

Publication Publication Date Title
US20120171683A1 (en) Analysis of fragmented genomic dna in droplets
US20200370097A1 (en) Quantitating high titer samples by digital pcr
US11612892B2 (en) Method of performing droplet-based assays
US10240187B2 (en) Stabilized droplets for calibration and testing
US20230211347A1 (en) Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis
EP2347245B1 (fr) Procédure d'essai à base des gouttes
US20130302792A1 (en) Calibrations and controls for droplet-based assays
US20140272996A1 (en) Droplet generator with collection tube
US20130084572A1 (en) Calibrations and controls for droplet-based assays
US11130128B2 (en) Detection method for a target nucleic acid
EP2970367B1 (fr) Essais numériques ayant un rapporteur générique
EP2542660A1 (fr) Chimie des émulsions et dosages pour gouttelettes encapsulées
CA2816702A1 (fr) Analyse d'adn genomique fragmente dans des gouttelettes
US20230372935A1 (en) Partition-based method of analysis
CN105112507A (zh) 微小rna的数字化恒温检测方法
US20180209874A1 (en) Assay performance systems including aqueous sample stabilization
Tupik et al. Fluorescent detection upon droplet amplification of nucleic acids

Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20161102