EP2635708A4 - Analyse d'adn génomique fragmenté dans des gouttelettes - Google Patents

Analyse d'adn génomique fragmenté dans des gouttelettes

Info

Publication number
EP2635708A4
EP2635708A4 EP11838712.5A EP11838712A EP2635708A4 EP 2635708 A4 EP2635708 A4 EP 2635708A4 EP 11838712 A EP11838712 A EP 11838712A EP 2635708 A4 EP2635708 A4 EP 2635708A4
Authority
EP
European Patent Office
Prior art keywords
droplets
analysis
genomic dna
fragmented genomic
fragmented
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11838712.5A
Other languages
German (de)
English (en)
Other versions
EP2635708A1 (fr
Inventor
Kevin D Ness
Amy L Hiddessen
Paul W Wyatt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/976,827 external-priority patent/US9598725B2/en
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of EP2635708A1 publication Critical patent/EP2635708A1/fr
Publication of EP2635708A4 publication Critical patent/EP2635708A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP11838712.5A 2010-11-01 2011-11-01 Analyse d'adn génomique fragmenté dans des gouttelettes Withdrawn EP2635708A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40910610P 2010-11-01 2010-11-01
US12/976,827 US9598725B2 (en) 2010-03-02 2010-12-22 Emulsion chemistry for encapsulated droplets
PCT/US2011/058854 WO2012061442A1 (fr) 2010-11-01 2011-11-01 Analyse d'adn génomique fragmenté dans des gouttelettes

Publications (2)

Publication Number Publication Date
EP2635708A1 EP2635708A1 (fr) 2013-09-11
EP2635708A4 true EP2635708A4 (fr) 2014-10-29

Family

ID=46024812

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11838712.5A Withdrawn EP2635708A4 (fr) 2010-11-01 2011-11-01 Analyse d'adn génomique fragmenté dans des gouttelettes

Country Status (7)

Country Link
EP (1) EP2635708A4 (fr)
JP (1) JP2013541347A (fr)
CN (1) CN103443289A (fr)
AU (1) AU2011323507A1 (fr)
CA (1) CA2816702A1 (fr)
SG (1) SG190076A1 (fr)
WO (1) WO2012061442A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11123740B2 (en) 2015-06-29 2021-09-21 Arizona Board Of Regents On Behalf Of Arizona State University Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis
US10760116B2 (en) 2015-08-28 2020-09-01 Kabushiki Kaisha Dnaform Analysis kit, analyzer, and methods for analyzing template nucleic acid or target substance
JP2020500517A (ja) * 2016-11-28 2020-01-16 アリゾナ ボード オブ リージェンツ オン ビハーフ オブ アリゾナ ステート ユニバーシティ 連続流小滴反応に関連するシステムおよび方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5555191A (en) * 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
WO2005043154A2 (fr) * 2003-10-27 2005-05-12 Massachusetts Institute Of Technology Chambres de réaction haute densité et procédés d'utilisation
CA2636855C (fr) * 2006-01-11 2016-09-27 Raindance Technologies, Inc. Dispositifs microfluidiques et leurs procedes d'utilisation dans la formation et le controle de nanoreacteurs
US8383345B2 (en) * 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Application Note: Targeted Sequencing of 4000 Exons Using the RainDance Technologies Sequence Enrichment Assay", APPLICATION NOTE FROM RAINDANCE, 2009, XP055138343 *
BENJAMIN J. HINDSON ET AL: "High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number", ANALYTICAL CHEMISTRY, vol. 83, no. 22, 28 October 2011 (2011-10-28), pages 8604 - 8610, XP055047554, ISSN: 0003-2700, DOI: 10.1021/ac202028g *
RYAN TEWHEY ET AL: "Microdroplet-based PCR enrichment for large-scale targeted sequencing", NATURE BIOTECHNOLOGY, vol. 27, no. 11, 1 November 2009 (2009-11-01), pages 1025 - 1031, XP055103848, ISSN: 1087-0156, DOI: 10.1038/nbt.1583 *
See also references of WO2012061442A1 *
ZHISHAN HUA ET AL: "Multiplexed Real-Time Polymerase Chain Reaction on a Digital Microfluidic Platform", ANALYTICAL CHEMISTRY, vol. 82, no. 6, 15 March 2010 (2010-03-15), pages 2310 - 2316, XP055138297, ISSN: 0003-2700, DOI: 10.1021/ac902510u *

Also Published As

Publication number Publication date
JP2013541347A (ja) 2013-11-14
EP2635708A1 (fr) 2013-09-11
AU2011323507A1 (en) 2013-06-13
CN103443289A (zh) 2013-12-11
CA2816702A1 (fr) 2012-05-10
SG190076A1 (en) 2013-06-28
WO2012061442A1 (fr) 2012-05-10

Similar Documents

Publication Publication Date Title
HK1247254A1 (zh) 核酸測序的方法和組合物
HK1171083A1 (zh) 核酸分析
EP2702175A4 (fr) Procédés et compositions pour l'analyse d'acide nucléique
EP2798090A4 (fr) Analyse d'acides nucléiques au moyen d'une pcr en émulsion
EP2484748A4 (fr) Analyseur d'acide nucléique
IL235128B (en) Preparation of a sample of nucleic acids
IL214034A (en) Nucleic Acid Analysis with Single Cell
ZA201205891B (en) Dna polymerases
EP2580353A4 (fr) Flux alternatifs de nucléotides dans des procédés de séquençage par synthèse
EP2764112A4 (fr) Analyse directe d'acides nucléiques
EP2798089A4 (fr) Procédés et compositions pour la mise en uvre de réactions d'amplification d'acide nucléique
EP2414545A4 (fr) Compositions constituées d'une préparation d'acides nucléiques et procédés associés
EP2553125A4 (fr) Outils et procédé pour séquençage d'acide nucléique dépendant du dégrafage de nanopores
ZA201303929B (en) Microfluidic device for nucleic acid extraction and fractionation
HK1186215A1 (zh) 基因分型
HK1193436A1 (zh) 核酸樣品的製備方法及組合物
EP2600156A4 (fr) Dispositif d'analyse d'acides nucléiques
GB201119903D0 (en) Nucleic acid production and sequence analysis
EP2798092A4 (fr) Sondes d'hybridation d'acide nucléique
EP2902488A4 (fr) Adn polymérase modifiée
EP2536739A4 (fr) Amorces et procédés pour l'amplification d'acide nucléique
EP2740804A4 (fr) Procédé d'analyse d'acide nucléique
LT2993232T (lt) Nukleorūgščių gavimo būdas
EP2451976A4 (fr) Nano-biocapteurs à acides nucléiques
PT2748320T (pt) Composições, métodos e kits para hibridação de ácidos nucleicos

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20130529

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20141001

RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/68 20060101AFI20140926BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN

18W Application withdrawn

Effective date: 20150415