EP2635708A4 - Analysis of fragmented genomic dna in droplets - Google Patents

Analysis of fragmented genomic dna in droplets

Info

Publication number
EP2635708A4
EP2635708A4 EP11838712.5A EP11838712A EP2635708A4 EP 2635708 A4 EP2635708 A4 EP 2635708A4 EP 11838712 A EP11838712 A EP 11838712A EP 2635708 A4 EP2635708 A4 EP 2635708A4
Authority
EP
European Patent Office
Prior art keywords
droplets
analysis
genomic dna
fragmented genomic
fragmented
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11838712.5A
Other languages
German (de)
French (fr)
Other versions
EP2635708A1 (en
Inventor
Kevin D Ness
Amy L Hiddessen
Paul W Wyatt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio-Rad Laboratories Inc
Original Assignee
Bio-Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US40910610P priority Critical
Priority to US12/976,827 priority patent/US9598725B2/en
Application filed by Bio-Rad Laboratories Inc filed Critical Bio-Rad Laboratories Inc
Priority to PCT/US2011/058854 priority patent/WO2012061442A1/en
Publication of EP2635708A1 publication Critical patent/EP2635708A1/en
Publication of EP2635708A4 publication Critical patent/EP2635708A4/en
Application status is Withdrawn legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
EP11838712.5A 2010-03-02 2011-11-01 Analysis of fragmented genomic dna in droplets Withdrawn EP2635708A4 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US40910610P true 2010-11-01 2010-11-01
US12/976,827 US9598725B2 (en) 2010-03-02 2010-12-22 Emulsion chemistry for encapsulated droplets
PCT/US2011/058854 WO2012061442A1 (en) 2010-11-01 2011-11-01 Analysis of fragmented genomic dna in droplets

Publications (2)

Publication Number Publication Date
EP2635708A1 EP2635708A1 (en) 2013-09-11
EP2635708A4 true EP2635708A4 (en) 2014-10-29

Family

ID=46024812

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11838712.5A Withdrawn EP2635708A4 (en) 2010-03-02 2011-11-01 Analysis of fragmented genomic dna in droplets

Country Status (7)

Country Link
EP (1) EP2635708A4 (en)
JP (1) JP2013541347A (en)
CN (1) CN103443289A (en)
AU (1) AU2011323507A1 (en)
CA (1) CA2816702A1 (en)
SG (1) SG190076A1 (en)
WO (1) WO2012061442A1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5555191A (en) * 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
US20050277125A1 (en) * 2003-10-27 2005-12-15 Massachusetts Institute Of Technology High-density reaction chambers and methods of use
EP2363205A3 (en) * 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
US8383345B2 (en) * 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Application Note: Targeted Sequencing of 4000 Exons Using the RainDance Technologies Sequence Enrichment Assay", APPLICATION NOTE FROM RAINDANCE, 2009, XP055138343 *
BENJAMIN J. HINDSON ET AL: "High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number", ANALYTICAL CHEMISTRY, vol. 83, no. 22, 28 October 2011 (2011-10-28), pages 8604 - 8610, XP055047554, ISSN: 0003-2700, DOI: 10.1021/ac202028g *
RYAN TEWHEY ET AL: "Microdroplet-based PCR enrichment for large-scale targeted sequencing", NATURE BIOTECHNOLOGY, vol. 27, no. 11, 1 November 2009 (2009-11-01), pages 1025 - 1031, XP055103848, ISSN: 1087-0156, DOI: 10.1038/nbt.1583 *
See also references of WO2012061442A1 *
ZHISHAN HUA ET AL: "Multiplexed Real-Time Polymerase Chain Reaction on a Digital Microfluidic Platform", ANALYTICAL CHEMISTRY, vol. 82, no. 6, 15 March 2010 (2010-03-15), pages 2310 - 2316, XP055138297, ISSN: 0003-2700, DOI: 10.1021/ac902510u *

Also Published As

Publication number Publication date
SG190076A1 (en) 2013-06-28
CN103443289A (en) 2013-12-11
CA2816702A1 (en) 2012-05-10
JP2013541347A (en) 2013-11-14
AU2011323507A1 (en) 2013-06-13
EP2635708A1 (en) 2013-09-11
WO2012061442A1 (en) 2012-05-10

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Legal Events

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A4 Despatch of supplementary search report

Effective date: 20141001

RIC1 Classification (correction)

Ipc: C12Q 1/68 20060101AFI20140926BHEP

18W Withdrawn

Effective date: 20150415