SG190076A1 - Analysis of fragmented genomic dna in droplets - Google Patents

Analysis of fragmented genomic dna in droplets

Info

Publication number
SG190076A1
SG190076A1 SG2013033238A SG2013033238A SG190076A1 SG 190076 A1 SG190076 A1 SG 190076A1 SG 2013033238 A SG2013033238 A SG 2013033238A SG 2013033238 A SG2013033238 A SG 2013033238A SG 190076 A1 SG190076 A1 SG 190076A1
Authority
SG
Singapore
Prior art keywords
droplets
genomic dna
fragmented
dna
per
Prior art date
Application number
SG2013033238A
Inventor
Kevin D Ness
Amy L Hiddessen
Paul W Wyatt
Original Assignee
Kevin D Ness
Amy L Hiddessen
Paul W Wyatt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US40910610P priority Critical
Priority to US12/976,827 priority patent/US9598725B2/en
Application filed by Kevin D Ness, Amy L Hiddessen, Paul W Wyatt filed Critical Kevin D Ness
Priority to PCT/US2011/058854 priority patent/WO2012061442A1/en
Publication of SG190076A1 publication Critical patent/SG190076A1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Abstract

Method of analyzing genomic DNA. Genomic DNA including a target may be obtained. The genomic DNA may be fragmented volitionally to produce fragmented DNA. The fragmented DNA may be passed through a droplet generator to generate aqueous droplets containing the fragmented DNA. An assay may be performed on the droplets to determine a level of the target. In some embodiments, the droplets may contain the genomic DNA at a concentration of at least about five nanograms per microliter, the droplets may be generated at a droplet generation frequency of at least about 50 droplets per second, the droplets may have an average volume of less than about 10 nanoliters per droplet, the droplets may generated at a flow rate of greater than about 50 nanoliters per second, or any combination thereof.
SG2013033238A 2010-03-02 2011-11-01 Analysis of fragmented genomic dna in droplets SG190076A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US40910610P true 2010-11-01 2010-11-01
US12/976,827 US9598725B2 (en) 2010-03-02 2010-12-22 Emulsion chemistry for encapsulated droplets
PCT/US2011/058854 WO2012061442A1 (en) 2010-11-01 2011-11-01 Analysis of fragmented genomic dna in droplets

Publications (1)

Publication Number Publication Date
SG190076A1 true SG190076A1 (en) 2013-06-28

Family

ID=46024812

Family Applications (1)

Application Number Title Priority Date Filing Date
SG2013033238A SG190076A1 (en) 2010-03-02 2011-11-01 Analysis of fragmented genomic dna in droplets

Country Status (7)

Country Link
EP (1) EP2635708A4 (en)
JP (1) JP2013541347A (en)
CN (1) CN103443289A (en)
AU (1) AU2011323507A1 (en)
CA (1) CA2816702A1 (en)
SG (1) SG190076A1 (en)
WO (1) WO2012061442A1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5555191A (en) * 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
WO2005043154A2 (en) * 2003-10-27 2005-05-12 Massachusetts Institute Of Technology High density reaction chambers and methods of use
EP2363205A3 (en) * 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
US8383345B2 (en) * 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9156010B2 (en) * 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system

Also Published As

Publication number Publication date
EP2635708A1 (en) 2013-09-11
WO2012061442A1 (en) 2012-05-10
AU2011323507A1 (en) 2013-06-13
CN103443289A (en) 2013-12-11
EP2635708A4 (en) 2014-10-29
JP2013541347A (en) 2013-11-14
CA2816702A1 (en) 2012-05-10

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