CN103430846A - Culture medium composition for strawberry tissue culture - Google Patents
Culture medium composition for strawberry tissue culture Download PDFInfo
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- CN103430846A CN103430846A CN2013103511288A CN201310351128A CN103430846A CN 103430846 A CN103430846 A CN 103430846A CN 2013103511288 A CN2013103511288 A CN 2013103511288A CN 201310351128 A CN201310351128 A CN 201310351128A CN 103430846 A CN103430846 A CN 103430846A
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Abstract
The invention discloses culture medium composition for strawberry tissue culture. During the whole strawberry growth process, matrix for basic growth and matrix for inducing growth and propagation growth are separately prepared, and with the matching of components in different proportions for the culture media, nutrient supply of the culture medium is optimized, the nutrient balance during the strawberry growth process is ensured, defects of overnutrition or undernutrition, which are easy to appear in each phase of the traditional culture medium, are effectively overcome, the rooting percentage of the strawberry and the stem survival rate are increased, and the seedling quality is improved.
Description
Technical field
The present invention relates to the formula of crops medium, relate in particular to the culture media composition that a kind of strawberry tissue is cultivated.
Background technology
Strawberry is rose family Fragaria perennial root perennial evergreen herbaceous plant, is worldwide fruit, also has certain medical value, and gastrointestinal disease, anaemia are had to certain curative effect.Strawberry is ripe fruit the earliest in a bit, the end of spring and the beginning of summer can be gathered and be put on market, its industrial chain is longer, can be processed into the various food such as fruit juice, jam, beverage, cake and preserved fruit, but strawberry production for a long time, all rely on traditional soil cultivation division propagation method to produce seedling, traditional soil cultivation method labour intensity is large, the problems such as fruiting period is short, continuous cropping obstacle become increasingly conspicuous, and having limited strawberry further develops; Along with developing rapidly of facilities horticulture, cultivation technique without soil is just in large tracts of land, apply on a large scale, some safety, high-quality, convenience, good composite cultivation substrate constantly are developed, synthetic and use, but required matrix and induced growth when existing medium is not grown basis in the overall process of strawberry growth, while expanding numerous growth, required matrix is separated and prepared, thereby caused in basic process of growth overnutrition and later stage induced growth malnourished phenomenon may occur.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the present invention proposes a kind of nutrient of can active balance strawberry tissue cultivating different growing stage, improve the culture media composition of a kind of strawberry tissue cultivation of rooting rate and stem apex survival rate.
Technical scheme: in order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: the culture media composition that a kind of strawberry tissue is cultivated: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight are as follows:
(1) basal medium: 1/4 improvement MS, gel 3-5g/L, pH is adjusted to 5-6;
(2) induced bud differential medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
(3) root induction medium: 1/2 improvement MS, gel 4-6g/L, hormone, pH is adjusted to 5.5-6;
(4) expand breeding culture medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
The solvent of the MS medium of described improvement is water, and composition comprises macroelement, trace element, molysite and organic matter, and concrete component and corresponding concentration are as follows:
Wherein macroelement comprises potassium nitrate 1700mg/L, ammonium nitrate 1600mg/L, epsom salt 300mg/L, anhydrous potassium dihydrogenphosphate 150mg/L, calcium chloride dihydrate 450mg/L and disodium ethylene diamine tetraacetate 37mg/L;
Wherein trace element comprises four water manganese sulphate 23.1mg/L, zinc sulphate 9mg/L, boric acid 5.9mg/L, potassium iodide 0.7mg/L, sodium molybdate 0.2mg/L, copper sulphate 0.2mg/L and cobalt oxide 0.03mg/L;
Wherein molysite is ferrous sulfate heptahydrate 26.5mg/L;
Wherein organic matter comprises inositol 100mg/L, glycine 3mg/L, nicotinic acid 0.5mg/L, sucrose 35g/L and nicotinic acid pyridoxine 0.5mg/L.
The mixture more preferred, that described gel is agar and gellan gum, mass ratio is 3:1.
Further, in described induced bud differential medium, hormone comprises 2,4-D0.3mg/L and 6BA0.3mg/L.
Further, in described root induction medium, hormone comprises NAA0.2mg/L and 6BA0.2mg/L.
Further, in described expansion breeding culture medium, hormone is 2,4-D0.2mg/L, NAA0.1mg/L and 6BA0.2mg/L.
Beneficial effect: the culture media composition that a kind of strawberry tissue provided by the invention is cultivated; required matrix and induced growth while in the overall process of strawberry growth, being grown in basis, while expanding numerous growth, required matrix is separated and is prepared; be equipped with different media components proportionings; optimized the medium nutrition supply; guaranteed the nutrient equilibrium in the strawberry process of growth; overnutrition or malnourished defect easily appear in the stages that has effectively overcome traditional medium; improve strawberry rooting rate and stem apex survival rate, improved Quality of Seedlings.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail:
Embodiment 1:
The culture media composition that a kind of strawberry tissue is cultivated: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight are as follows:
(1) basal medium: mixture (mass ratio the is 3:1) 3g/L of 1/4 improvement MS, agar and gellan gum, pH is adjusted to 5.0;
(2) induced bud differential medium: mixture (mass ratio the is 3:1) 6g/L, 2 of improvement MS, agar and gellan gum, 4-D0.3mg/L, 6BA0.3mg/L, pH is adjusted to 5.5;
(3) root induction medium: mixture (mass ratio is 3:1) 4g/L, NAA0.2mg/L, the 6BA0.2mg/L of 1/2 improvement MS, agar and gellan gum, pH is adjusted to 5.5;
(4) expand breeding culture medium: mixture (mass ratio the is 3:1) 6g/L, 2 of improvement MS, agar and gellan gum, 4-D0.2mg/L, NAA0.1mg/L, 6BA0.2mg/L, pH is adjusted to 5.5.
Embodiment 2:
The culture media composition that a kind of strawberry tissue is cultivated: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight are as follows:
(1) basal medium: mixture (mass ratio the is 3:1) 5g/L of 1/4 improvement MS, agar and gellan gum, pH is adjusted to 6.0;
(2) induced bud differential medium: mixture (mass ratio the is 3:1) 8g/L, 2 of improvement MS, agar and gellan gum, 4-D0.3mg/L, 6BA0.3mg/L, pH is adjusted to 6.0;
(3) root induction medium: mixture (mass ratio is 3:1) 6g/L, NAA0.2mg/L, the 6BA0.2mg/L of 1/2 improvement MS, agar and gellan gum,, pH is adjusted to 6.0;
(4) expand breeding culture medium: mixture (mass ratio is 3:1) 8g/L, the 6g/L, 2 of improvement MS, agar and gellan gum, 4-D0.2mg/L, NAA0.1mg/L, 6BA0.2mg/L, pH is adjusted to 6.0.
Embodiment 3:
The culture media composition that a kind of strawberry tissue is cultivated: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight are as follows:
(1) basal medium: mixture (mass ratio the is 3:1) 4g/L of 1/4 improvement MS, agar and gellan gum, pH is adjusted to 5.5;
(2) induced bud differential medium: mixture (mass ratio the is 3:1) 7g/L, 2 of improvement MS, agar and gellan gum, 4-D0.3mg/L, 6BA0.3mg/L, pH is adjusted to 5.7;
(3) root induction medium: mixture (mass ratio is 3:1) 5g/L, NAA0.2mg/L, the 6BA0.2mg/L of 1/2 improvement MS, agar and gellan gum,, pH is adjusted to 5.7;
(4) expand breeding culture medium: mixture (mass ratio is 3:1) 7g/L, the 6g/L, 2 of improvement MS, agar and gellan gum, 4-D0.2mg/L, NAA0.1mg/L, 6BA0.2mg/L, pH is adjusted to 5.7.
In above-described embodiment 1-3, the solvent of the MS medium of described improvement is water, and composition comprises macroelement, trace element, molysite and organic matter, and concrete component and corresponding concentration are as follows:
Wherein macroelement comprises potassium nitrate 1700mg/L, ammonium nitrate 1600mg/L, epsom salt 300mg/L, anhydrous potassium dihydrogenphosphate 150mg/L, calcium chloride dihydrate 450mg/L and disodium ethylene diamine tetraacetate 37mg/L;
Wherein trace element comprises four water manganese sulphate 23.1mg/L, zinc sulphate 9mg/L, boric acid 5.9mg/L, potassium iodide 0.7mg/L, sodium molybdate 0.2mg/L, copper sulphate 0.2mg/L and cobalt oxide 0.03mg/L;
Wherein molysite is ferrous sulfate heptahydrate 26.5mg/L;
Wherein organic matter comprises inositol 100mg/L, glycine 3mg/L, nicotinic acid 0.5mg/L, sucrose 35g/L and nicotinic acid pyridoxine 0.5mg/L.
The 5-6 month, select the afternoon of fine day, choose 180 of the stem apexs of 0.3-0.5mm on the stolon of just having extracted out healthy and strong, disease-free strawberry is cultivated respectively as the strawberry explant on 3 described medium of embodiment, condition of culture for carrying out illumination cultivation at 25 ± 2 ℃ of temperature, illumination in 16 hours, intensity of illumination 10～30 μ m
-2.s
-1; Be 20 ± 2 ℃ in temperature and secretly cultivate, secretly cultivate 6 hours, above-mentioned illumination and dark the cultivation are hocketed.
The culture media composition that uses a kind of strawberry tissue of the present invention to cultivate is cultivated; guaranteed the nutrient equilibrium in the strawberry process of growth; overnutrition or malnourished defect easily appear in the stages that has effectively overcome traditional medium; the more traditional medium of strawberry rooting rate has improved 8.9-10.5%; the stem apex survival rate has improved 7.8-9.6%; Quality of Seedlings obviously improves, and leaf is green straight and upright.
Should be understood that, above embodiment only is not used in and limits the scope of the invention for the present invention is described, after having read the present invention, those skilled in the art all fall within the application's claims limited range to the modification of the various equivalent form of values of the present invention.
Claims (5)
1. the culture media composition that a strawberry tissue is cultivated, it is characterized in that comprising basal medium, inducing culture and expansion breeding culture medium: each component and contained weight are as follows:
(1) basal medium: 1/4 improvement MS, gel 3-5g/L, pH is adjusted to 5-6;
(2) induced bud differential medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
(3) root induction medium: 1/2 improvement MS, gel 4-6g/L, hormone, pH is adjusted to 5.5-6;
(4) expand breeding culture medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
The solvent of the MS medium of described improvement is water, and composition comprises macroelement, trace element, molysite and organic matter, and concrete component and corresponding concentration are as follows:
Wherein macroelement comprises potassium nitrate 1700mg/L, ammonium nitrate 1600mg/L, epsom salt 300mg/L, anhydrous potassium dihydrogenphosphate 150mg/L, calcium chloride dihydrate 450mg/L and disodium ethylene diamine tetraacetate 37mg/L;
Wherein trace element comprises four water manganese sulphate 23.1mg/L, zinc sulphate 9mg/L, boric acid 5.9mg/L, potassium iodide 0.7mg/L, sodium molybdate 0.2mg/L, copper sulphate 0.2mg/L and cobalt oxide 0.03mg/L;
Wherein molysite is ferrous sulfate heptahydrate 26.5mg/L;
Wherein organic matter comprises inositol 100mg/L, glycine 3mg/L, nicotinic acid 0.5mg/L, sucrose 35g/L and nicotinic acid pyridoxine 0.5mg/L.
2. the culture media composition that a kind of strawberry tissue according to claim 1 is cultivated, it is characterized in that: the mixture that described gel is agar and gellan gum, mass ratio is 3:1.
3. the culture media composition that a kind of strawberry tissue according to claim 1 is cultivated, it is characterized in that: in described induced bud differential medium, hormone comprises 2,4-D0.3mg/L and 6BA0.3mg/L.
4. the culture media composition that a kind of strawberry tissue according to claim 1 is cultivated, it is characterized in that: in described root induction medium, hormone comprises NAA0.2mg/L and 6BA0.2mg/L.
5. the culture media composition that a kind of strawberry tissue according to claim 1 is cultivated, it is characterized in that: in described expansion breeding culture medium, hormone is 2,4-D0.2mg/L, NAA0.1mg/L and 6BA0.2mg/L.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018068281A1 (en) * | 2016-10-14 | 2018-04-19 | 泉州市泉美生物科技有限公司 | Method for cultivating plant in transparent sealed container and medium therefor |
| CN117305217A (en) * | 2023-10-24 | 2023-12-29 | 临沂市农业科学院 | Optimized stem tip tissue culture medium, culture method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946702A (en) * | 2010-08-13 | 2011-01-19 | 北京金六环农业园 | Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings |
| CN102893869A (en) * | 2012-10-22 | 2013-01-30 | 浙江省农业科学院 | Root tip detoxification and rapid propagation technology of strawberries |
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2013
- 2013-08-13 CN CN201310351128.8A patent/CN103430846B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946702A (en) * | 2010-08-13 | 2011-01-19 | 北京金六环农业园 | Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings |
| CN102893869A (en) * | 2012-10-22 | 2013-01-30 | 浙江省农业科学院 | Root tip detoxification and rapid propagation technology of strawberries |
Non-Patent Citations (4)
| Title |
|---|
| A.J.PASSEY ET AL: "Adventitious shoot regeneration fromseven commercial strawberry cultivars (Fragaria × ananassa Duch.) using arange ofexplant types", 《PLANT CELL REP 》 * |
| INDRAD.BHATT ET AL: "Micropropagation of Indian wild strawberry", 《PLANT CELL,TISSUE AND ORGAN CULTURE》 * |
| 李慧: "草莓优新品种的组织培养", 《中国果菜》 * |
| 董晓颖等: "哈尼草莓叶片诱导再生植株及快速繁殖研究", 《莱阳农学院学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018068281A1 (en) * | 2016-10-14 | 2018-04-19 | 泉州市泉美生物科技有限公司 | Method for cultivating plant in transparent sealed container and medium therefor |
| CN117305217A (en) * | 2023-10-24 | 2023-12-29 | 临沂市农业科学院 | Optimized stem tip tissue culture medium, culture method and application |
| CN117305217B (en) * | 2023-10-24 | 2024-08-06 | 临沂市农业科学院 | Optimized stem tip tissue culture medium, culture method and application |
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Effective date of registration: 20210105 Address after: 225300 No.25 Chunhui Road, Zhangguo Town, Xinghua City, Taizhou City, Jiangsu Province Patentee after: Xinghua Lingyi science and Technology Consulting Service Co.,Ltd. Address before: Room 1406, 14 / F, unit 1, building 5, No. 366, Ronghua South Road, high tech Zone, Chengdu, Sichuan 610000 Patentee before: Sichuan qiyibiao Information Technology Co.,Ltd. Effective date of registration: 20210105 Address after: Room 1406, 14 / F, unit 1, building 5, No. 366, Ronghua South Road, high tech Zone, Chengdu, Sichuan 610000 Patentee after: Sichuan qiyibiao Information Technology Co.,Ltd. Address before: No.112 NInghang Road, Huayang Town, Jurong City, Zhenjiang City, Jiangsu Province Patentee before: ZHENJIANG AGRICULTURAL SCIENCE & TECHNOLOGY INDUSTRIAL Co. |
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Effective date of registration: 20211026 Address after: 750000 group 3, Hongxi village, Hongguang Town, Helan County, Yinchuan City, Ningxia Hui Autonomous Region Patentee after: He Cong Address before: 225300 No.25 Chunhui Road, Zhangguo Town, Xinghua City, Taizhou City, Jiangsu Province Patentee before: Xinghua Lingyi science and Technology Consulting Service Co.,Ltd. |