CN105961195B - A kind of hybridizing method of high anthocyanidin charcoal black hull peanut - Google Patents

A kind of hybridizing method of high anthocyanidin charcoal black hull peanut Download PDF

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Publication number
CN105961195B
CN105961195B CN201610510330.4A CN201610510330A CN105961195B CN 105961195 B CN105961195 B CN 105961195B CN 201610510330 A CN201610510330 A CN 201610510330A CN 105961195 B CN105961195 B CN 105961195B
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peanut
anthocyanidin
maternal
charcoal black
charcoal
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CN105961195A (en
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王国宣
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a kind of hybridizing methods of high anthocyanidin charcoal black hull peanut, include the following steps:1) 5 are set using peanut as maternal orthogonal area, 5 using black soybean as maternal anti-hybridization region;2) orthogonal area and anti-hybridization region are 5 cells, and 23 28 seeds are planted in each cell, and each cell selects and remain 20 young plants as maternal after emergence;3) 0.5 mu of crop field peanut and black soybean as male parent is planted;4) 100 plants of healthy seedling is selected and remain in maternal area altogether, and emasculation work before being pollinated by stages and in groups is capped carton closing after pollination;5) 6 42 seedlings of cell in peanut hybridization garden point are set up to continue to hybridize, is selected from cross combination system and carries out orthogonal breeding different from original Series of Peanut character;Using the high anthocyanidin charcoal black hull peanut that hybridization technique of the present invention is planted per 100g peanut grain 419 ㎎ containing anthocyanidin, the blank of Chinese prevention and control cancer, wide market have been filled up.

Description

A kind of hybridizing method of high anthocyanidin charcoal black hull peanut
Technical field
The present invention relates to agricultural cultivation technical field, the hybridizing method of specifically a kind of high anthocyanidin charcoal black hull peanut.
Background technology
Anthocyanidin is anti-oxidant object, is the sole material for inhibiting free radical, is special plant coloured corpuscle liquid, derives from For chromosome by fracture, the chromatoplastid formed in displacement and reproduction process is converted to anthocyanidin, anthocyanidin to plant in heredity It is substance global rare and in short supply, since anthocyanidin resource is deficient scarce and precious, so nobody is beneficial to man to anthocyanidin Inhibit the health care Journal of Sex Research of free radical and anti-cancer plus benefit supplement;Anthocyanidin is inhibited in blood of human body and cell since long-term eats With chemical fertilizer, pesticide, hormone, Additive Production agricultural product in dive have endotoxin contamination object accumulation and formed free radical, freely Base is the old complaint of cancer, is the source of incurable disease, is the carrier of a variety of malignant diseases;The anti-cancer principle of anthocyanidin is:He is to remove The free radical that the bad factor of pollution in blood of human body and cell is formed.
Peanut anthocyanidin is a kind of addition type anthocyanidin, and original red, purple two chromoplast is increased to three colors of black substance body Property addition type anthocyanidin;That is discussed in theory is mended to the benefit of three color plant plastids according to classic traditional Chinese medicine:Red plastid channel tropism Yu Xin, the heart are the internal organs of blood vessels origin, and red plastid has the effect of raw arteries and veins hematopoiesis;For black plastid channel tropism in kidney, kidney is that marrow essence is consolidated The internal organs of sheet, black substance body have the effect of clever ear of dark hair crow palpus lengthens one's life;Purple plastid channel tropism is in liver, and liver is the internal organs of library blood, purple matter Body has the effect of improving eyesight is invigorated blood circulation;So black substance body peanut anthocyanidin has and inhibits plus economic benefits and social benefits sexual clorminance that benefit is mended, more than its 20 The excellent benefit for kind being beneficial to growth in humans's development is one to cut plant the precious resources do not replaced;Peanut anthocyanidin is Chinese unique The anthocyanidin field crops that can be planted throughout the country can fill up the deficient scarce contradiction of global anthocyanidin resource, therefore Has historic important meaning for the health care and good health and a long life of the global people for the research and experiment that improve peanut anthocyanidin Justice, application prospect are unlimited.
Invention content
The purpose of the present invention is to provide a kind of hybridizing method of high anthocyanidin charcoal black hull peanut, to solve above-mentioned background skill The problem of being proposed in art.
To achieve the above object, the present invention provides following technical solution:
A kind of hybridizing method of high anthocyanidin charcoal black hull peanut, includes the following steps:
1) 5 are set up using peanut as maternal orthogonal area, 5 using black soybean as maternal anti-hybridization region;
2) orthogonal area and anti-hybridization region are 5 cells, and 23-28 seeds are planted in each cell, each small after emergence 20 young plants are selected and remain as maternal in area;
3) needs of pollen are collected according to male parent, plant 0.5 mu of crop field peanut and black soybean as male parent respectively;
4) 100 plants of healthy seedling is selected and remain in maternal area altogether, emasculation work before being pollinated, and emasculation work uses goes by stages and in groups Hero, first awards the pollen of harvest on female head with cotton balls with paper bag, bestowed by heaven 2-3 wheat-middlings according to one, a female continuous 2-3 of head It pollinates repeatedly, meets the then pollination for completing a female head in 2-3 days of low temperature overcast and rainy day weather;Carton closing is capped after pollination;
5) 6 42 seedlings of cell in peanut hybridization garden point are set up to continue to hybridize, charcoal casting skin, flower are selected from cross combination system Speckle, golden yellow, purple etc. are different from original Series of Peanut character, carry out orthogonal breeding and obtain high anthocyanidin charcoal black hull peanut.
As further embodiment of the present invention:In the step 4, pollination time selects at 1 point in 9 points-afternoon of morning.
As further scheme of the invention:The high anthocyanidin charcoal black hull peanut planted in the step 5 is every 100g peanut grains 419 ㎎ containing anthocyanidin.
As further scheme of the invention:The high anthocyanidin charcoal black hull peanut hundred planted in the step 5 Peanut weight >=130g.
As further scheme of the invention:The high anthocyanidin charcoal black hull peanut per mu yield planted in the step 5 Amount >=300kg.
Compared with prior art, the beneficial effects of the invention are as follows:The high anthocyanidin charcoal black hull peanut of the present invention is in tradition On the basis of red, purple dichromatism anthocyanidin, increase black substance body constitute it is three colors, enhancing highly enriched, high-content novel Anthocyanidin organic molecule forms the novel anthocyanidin peanut of concentration property, and peanut of the invention is from budding to seedling to entire growth The cane of phase and petiole part are aubergine, and the fruit by growing aubergine after red core flower and purple yellow pollen grain pollination Needle finally lower grows charcoal casting skin, surface in the high peanut of the anthocyanidin content of thorn grain;The charcoal black hull peanut of the present invention is black Charcoal is such as painted, fruit particle is big, plumpness is high, and per mu yield is up to 300 kilograms;Abnormal mark flower with aubergine belongs to safflower lobe system In the high anthocyanidin crop of colouredness, good appearance;The high anthocyanidin charcoal black hull peanut of the present invention is planted convenient for cultivating, and is adapted to complete State's various regions field planting, anthocyanidin content is high, can fill up the deficient scarce contradiction of global anthocyanidin resource;The carbon black ginned cotton of the present invention Raw anthocyanidin content is high, delicate mouthfeel, and for taste preference also above the mouthfeel of existing peanut, charcoal black hull peanut is for Chinese crop field agriculture Crop has filled up the blank of Chinese prevention and control cancer, market in adjustment industry and the innovation species that inventory and supply side is gone to reform It has a extensive future.
Specific embodiment
The technical solution of this patent is described in more detail With reference to embodiment.
Embodiment 1
A kind of hybridizing method of high anthocyanidin charcoal black hull peanut, includes the following steps:
1) 5 are set up using peanut as maternal orthogonal area, 5 using black soybean as maternal anti-hybridization region;
2) orthogonal area and anti-hybridization region are 5 cells, and 23-28 seeds are planted in each cell, each small after emergence 20 young plants are selected and remain as maternal in area;
3) needs of pollen are collected according to male parent, plant 0.5 mu of crop field peanut and black soybean as male parent respectively;
4) 100 plants of healthy seedling is selected and remain in maternal area altogether, emasculation work before being pollinated, and emasculation work uses goes by stages and in groups Hero, first awards the pollen of harvest on female head with cotton balls with paper bag, bestowed by heaven 2-3 wheat-middlings according to one, a female continuous 2-3 of head It pollinates repeatedly, pollination time selection at 1 point in 9 points-afternoon in the morning, and meeting low temperature overcast and rainy day weather, then 2-3 days one female heads of completion are awarded Powder;Carton closing is capped after pollination;
5) 6 42 seedlings of cell in peanut hybridization garden point are set up to continue to hybridize, charcoal casting skin, flower are selected from cross combination system Speckle, golden yellow, purple etc. are different from original Series of Peanut character, carry out orthogonal breeding and obtain high anthocyanidin charcoal black hull peanut.
Using the high anthocyanidin charcoal black hull peanut of hybridization technique training of the present invention through agricultural product supervision test center of the Ministry of Agriculture Every hg peanut grains 419 ㎎ containing anthocyanidin is checked and examined out, hundred peanut weight are up to 130g, and per mu yield is up to 300 kilograms, nothing after multipoint relaying Disease occurs, and suitability is strong, and every plot that can plant common peanut can plant the high anthocyanidin charcoal black hull peanut of the present invention.
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party Formula, can also be under the premise of this patent objective not be departed from the knowledge that one skilled in the relevant art has Various changes can be made.

Claims (5)

1. a kind of hybridizing method of high anthocyanidin charcoal black hull peanut, which is characterized in that include the following steps:
1) 5 are set up using peanut as maternal orthogonal area, 5 using black soybean as maternal anti-hybridization region;
2) orthogonal area and anti-hybridization region are 5 cells, and 23-28 seeds are planted in each cell, each cell choosing after emergence 20 young plants are stayed as maternal;
3) needs of pollen are collected according to male parent, plant 0.5 mu of crop field peanut and black soybean as male parent respectively;
4) 100 plants of healthy seedling is selected and remain in maternal area altogether, and emasculation work before being pollinated, emasculation work uses emasculation by stages and in groups, the A batch awards the pollen of harvest on female head with cotton balls with paper bag, bestowed by heaven 2-3 wheat-middlings according to one, and continuous 2-3 days of female head is anti- The then pollination for completing a female head in 2-3 days of low temperature overcast and rainy day weather is met in multiple pollination;Carton closing is capped after pollination;
5) 6 42 seedlings of cell in peanut hybridization garden point are set up to continue to hybridize, charcoal casting skin, piebald are selected from cross combination system Line, golden yellow, purple etc. are different from original Series of Peanut character, carry out orthogonal breeding and obtain high anthocyanidin charcoal black hull peanut.
2. the hybridizing method of high anthocyanidin charcoal black hull peanut according to claim 1, which is characterized in that in the step 4, Pollination time selects at 1 point in 9 points-afternoon of morning.
3. the hybridizing method of high anthocyanidin charcoal black hull peanut according to claim 1, which is characterized in that in the step 5 Obtained high anthocyanidin charcoal black hull peanut is planted per 100g peanut grains 419 ㎎ containing anthocyanidin.
4. the hybridizing method of high anthocyanidin charcoal black hull peanut according to claim 1, which is characterized in that in the step 5 Plant obtained hundred peanut weight >=130g of high anthocyanidin charcoal black hull peanut.
5. the hybridizing method of high anthocyanidin charcoal black hull peanut according to claim 1, which is characterized in that in the step 5 Plant obtained high anthocyanidin charcoal black hull peanut per mu yield >=300kg.
CN201610510330.4A 2016-07-02 2016-07-02 A kind of hybridizing method of high anthocyanidin charcoal black hull peanut Expired - Fee Related CN105961195B (en)

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CN117568387A (en) * 2023-11-21 2024-02-20 山东省农业科学院 Method for improving peanut anthocyanin content by utilizing gene editing

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1922981A (en) * 2006-09-21 2007-03-07 陈新华 Selection and breeding method for black hull peanut
WO2010108312A1 (en) * 2009-03-23 2010-09-30 山东省花生研究所 A breeding method of peanut with high content of oleic acid and high yield
CN102217474A (en) * 2011-04-13 2011-10-19 李先明 Series flower peanuts, cultivation of series tour spectacle flower peanuts and natural crossing cultivation method
US8178752B1 (en) * 2011-06-14 2012-05-15 University Of Florida Research Foundation, Inc. Peanut cultivar UFT113
CN105284604A (en) * 2015-12-08 2016-02-03 广东省农业科学院作物研究所 Breeding method for high-yield black peanut variety

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1922981A (en) * 2006-09-21 2007-03-07 陈新华 Selection and breeding method for black hull peanut
WO2010108312A1 (en) * 2009-03-23 2010-09-30 山东省花生研究所 A breeding method of peanut with high content of oleic acid and high yield
CN102217474A (en) * 2011-04-13 2011-10-19 李先明 Series flower peanuts, cultivation of series tour spectacle flower peanuts and natural crossing cultivation method
US8178752B1 (en) * 2011-06-14 2012-05-15 University Of Florida Research Foundation, Inc. Peanut cultivar UFT113
CN105284604A (en) * 2015-12-08 2016-02-03 广东省农业科学院作物研究所 Breeding method for high-yield black peanut variety

Non-Patent Citations (4)

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Title
Evaluation of a peanut collection for shell-colour traits in two diverse soil types;Ran Hovav, et al.;《Plant Breeding》;20121231(第131期);第148-154页 *
Inheritance of Black-Pod color in Peanut;W.D.Branch, et al.;《The Journal of Heredity》;19971231;第88卷(第2期);第156-158页 *
大豆与花生远缘杂交简报;丹东市农业科学研究所;《辽宁农业科技》;19730829;第47-48,21页 *
黑花生品质评价及育种策略研究;陈新华等;《中国种业》;20061231(第11期);第17-18页 *

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