CN103430846B - Culture medium composition for strawberry tissue culture - Google Patents
Culture medium composition for strawberry tissue culture Download PDFInfo
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- CN103430846B CN103430846B CN201310351128.8A CN201310351128A CN103430846B CN 103430846 B CN103430846 B CN 103430846B CN 201310351128 A CN201310351128 A CN 201310351128A CN 103430846 B CN103430846 B CN 103430846B
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Abstract
The invention discloses culture medium composition for strawberry tissue culture. During the whole strawberry growth process, matrix for basic growth and matrix for inducing growth and propagation growth are separately prepared, and with the matching of components in different proportions for the culture media, nutrient supply of the culture medium is optimized, the nutrient balance during the strawberry growth process is ensured, defects of overnutrition or undernutrition, which are easy to appear in each phase of the traditional culture medium, are effectively overcome, the rooting percentage of the strawberry and the stem survival rate are increased, and the seedling quality is improved.
Description
Technical field
The present invention relates to the formula of crops medium, particularly relate to a kind of culture media composition of Strawberry tissue culture.
Background technology
Strawberry is rose family Fragaria perennial root perennial evergreen herbaceous plant, is worldwide fruit, also has certain medical value, have certain curative effect to gastrointestinal disease, anaemia.Strawberry is ripe fruit the earliest in a bit, the end of spring and the beginning of summer can carry out gathering putting on market, its industrial chain is longer, the various food such as fruit juice, jam, beverage, cake and preserved fruit can be processed into, but strawberry production for a long time, all rely on traditional soil cultivation division propagation method to produce seedling, the problems such as traditional soil cultivation method labour intensity is large, fruiting period is short, continuous cropping obstacle become increasingly conspicuous, and limiting strawberry further develops; Along with developing rapidly of facilities horticulture, cultivation technique without soil is just in large area, apply on a large scale, some safety, high-quality, convenience, good composite cultivation substrate are constantly developed, synthesize and use, but existing medium in the overall process that strawberry grows not by matrix required during based growth and induced growth, expand numerous growth time required matrix separate and prepare, thus result in the superfluous and later stage induced growth of based growth process Middle nutrition and may occur malnourished phenomenon.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the present invention proposes a kind of can active balance Strawberry tissue culture different growing stage nutrient, improve the culture media composition of a kind of Strawberry tissue culture of rooting rate and stem apex survival rate.
Technical scheme: in order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of culture media composition of Strawberry tissue culture: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: 1/4 improvement MS, gel 3-5g/L, pH is adjusted to 5-6;
(2) induced bud differential medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
(3) root induction medium: 1/2 improvement MS, gel 4-6g/L, hormone, pH is adjusted to 5.5-6;
(4) expand breeding culture medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
The solvent of the MS medium of described improvement is water, and composition comprises macroelement, trace element, molysite and organic matter, concrete component and corresponding concentration as follows:
Wherein macroelement comprises potassium nitrate 1700mg/L, ammonium nitrate 1600mg/L, epsom salt 300mg/L, anhydrous potassium dihydrogenphosphate 150mg/L, calcium chloride dihydrate 450mg/L and disodium ethylene diamine tetraacetate 37mg/L;
Its medium trace element comprises four water manganese sulphate 23.1mg/L, zinc sulphate 9mg/L, boric acid 5.9mg/L, potassium iodide 0.7mg/L, sodium molybdate 0.2mg/L, copper sulphate 0.2mg/L and cobalt oxide 0.03mg/L;
Wherein molysite is ferrous sulfate heptahydrate 26.5mg/L;
Wherein organic matter comprises inositol 100mg/L, glycine 3mg/L, nicotinic acid 0.5mg/L, sucrose 35g/L and nicotinic acid pyridoxine 0.5mg/L.
More preferred, described gel is the mixture of agar and gellan gum, and mass ratio is 3:1.
Further, in described induced bud differential medium, hormone comprises 2,4-D0.3mg/L and 6BA0.3mg/L.
Further, in described root induction medium, hormone comprises NAA0.2mg/L and 6BA0.2mg/L.
Further, in described expansion breeding culture medium, hormone is 2,4-D0.2mg/L, NAA0.1mg/L and 6BA0.2mg/L.
Beneficial effect: the culture media composition of a kind of Strawberry tissue culture provided by the invention; in the overall process of strawberry growth by matrix required during based growth and induced growth, expand numerous growth time required matrix separate and prepare; be equipped with different media components proportionings; optimize medium nutrition supply; ensure that the nutrient in strawberry process of growth is balanced; easily there is overnutrition or malnourished defect in each stage effectively overcoming conventional medium; improve strawberry rooting rate and stem apex survival rate, improve Quality of Seedlings.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail:
Embodiment 1:
A kind of culture media composition of Strawberry tissue culture: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: mixture (mass ratio is 3:1) 3g/L, the pH of 1/4 improvement MS, agar and gellan gum are adjusted to 5.0;
(2) induced bud differential medium: mixture (mass ratio the is 3:1) 6g/L of improvement MS, agar and gellan gum, 2,4-D0.3mg/L, 6BA0.3mg/L, pH be adjusted to 5.5;
(3) root induction medium: mixture (mass ratio is 3:1) 4g/L, NAA0.2mg/L, 6BA0.2mg/L of 1/2 improvement MS, agar and gellan gum, pH is adjusted to 5.5;
(4) expand breeding culture medium: improvement MS, agar and gellan gum mixture (mass ratio is 3:1) 6g/L, 2,4-D0.2mg/L, NAA0.1mg/L, 6BA0.2mg/L, pH is adjusted to 5.5.
Embodiment 2:
A kind of culture media composition of Strawberry tissue culture: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: mixture (mass ratio is 3:1) 5g/L, the pH of 1/4 improvement MS, agar and gellan gum are adjusted to 6.0;
(2) induced bud differential medium: mixture (mass ratio the is 3:1) 8g/L of improvement MS, agar and gellan gum, 2,4-D0.3mg/L, 6BA0.3mg/L, pH be adjusted to 6.0;
(3) root induction medium: mixture (mass ratio is 3:1) 6g/L, NAA0.2mg/L, 6BA0.2mg/L of 1/2 improvement MS, agar and gellan gum, pH is adjusted to 6.0;
(4) expand breeding culture medium: improvement MS, agar and gellan gum mixture (mass ratio is 3:1) 8g/L, 6g/L, 2,4-D0.2mg/L, NAA0.1mg/L, 6BA0.2mg/L, pH is adjusted to 6.0.
Embodiment 3:
A kind of culture media composition of Strawberry tissue culture: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: mixture (mass ratio is 3:1) 4g/L, the pH of 1/4 improvement MS, agar and gellan gum are adjusted to 5.5;
(2) induced bud differential medium: mixture (mass ratio the is 3:1) 7g/L of improvement MS, agar and gellan gum, 2,4-D0.3mg/L, 6BA0.3mg/L, pH be adjusted to 5.7;
(3) root induction medium: mixture (mass ratio is 3:1) 5g/L, NAA0.2mg/L, 6BA0.2mg/L of 1/2 improvement MS, agar and gellan gum, pH is adjusted to 5.7;
(4) expand breeding culture medium: improvement MS, agar and gellan gum mixture (mass ratio is 3:1) 7g/L, 6g/L, 2,4-D0.2mg/L, NAA0.1mg/L, 6BA0.2mg/L, pH is adjusted to 5.7.
In above-described embodiment 1-3, the solvent of the MS medium of described improvement is water, and composition comprises macroelement, trace element, molysite and organic matter, concrete component and corresponding concentration as follows:
Wherein macroelement comprises potassium nitrate 1700mg/L, ammonium nitrate 1600mg/L, epsom salt 300mg/L, anhydrous potassium dihydrogenphosphate 150mg/L, calcium chloride dihydrate 450mg/L and disodium ethylene diamine tetraacetate 37mg/L;
Its medium trace element comprises four water manganese sulphate 23.1mg/L, zinc sulphate 9mg/L, boric acid 5.9mg/L, potassium iodide 0.7mg/L, sodium molybdate 0.2mg/L, copper sulphate 0.2mg/L and cobalt oxide 0.03mg/L;
Wherein molysite is ferrous sulfate heptahydrate 26.5mg/L;
Wherein organic matter comprises inositol 100mg/L, glycine 3mg/L, nicotinic acid 0.5mg/L, sucrose 35g/L and nicotinic acid pyridoxine 0.5mg/L.
The 5-6 month, select the afternoon of fine day, the stem apex 180 that the stolon that healthy and strong, disease-free strawberry have just been extracted out is chosen 0.3-0.5mm is cultivated respectively as strawberry explant on the medium described in 3 embodiments, condition of culture for carry out illumination cultivation at temperature 25 ± 2 DEG C, 16 h light, intensity of illumination 10 ~ 30 μm
-2.s
-1; Be 20 ± 2 DEG C in temperature and carry out light culture, light culture 6 hours, above-mentioned illumination and light culture hocket.
The culture media composition of a kind of Strawberry tissue culture of the present invention is used to cultivate; ensure that the nutrient in strawberry process of growth is balanced; easily there is overnutrition or malnourished defect in each stage effectively overcoming conventional medium; strawberry rooting rate comparatively conventional medium improves 8.9-10.5%; stem apex survival rate improves 7.8-9.6%; Quality of Seedlings significantly improves, and leaf is green straight and upright.
Should be understood that, above embodiment is only not used in for illustration of the present invention and limits the scope of the invention, after having read the present invention, the amendment of those skilled in the art to the various equivalent form of value of the present invention has all fallen within the application's claims limited range.
Claims (2)
1. a culture media composition for Strawberry tissue culture, is characterized in that comprising basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: 1/4 improvement MS, gel 3-5g/L, pH is adjusted to 5-6;
(2) induced bud differential medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
(3) root induction medium: 1/2 improvement MS, gel 4-6g/L, hormone, pH is adjusted to 5.5-6;
(4) expand breeding culture medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
The solvent of the MS medium of described improvement is water, and composition comprises macroelement, trace element, molysite and organic matter, concrete component and corresponding concentration as follows:
Wherein macroelement comprises potassium nitrate 1700mg/L, ammonium nitrate 1600mg/L, epsom salt 300mg/L, anhydrous potassium dihydrogenphosphate 150mg/L, calcium chloride dihydrate 450mg/L and disodium ethylene diamine tetraacetate 37mg/L;
Its medium trace element comprises four water manganese sulphate 23.1mg/L, zinc sulphate 9mg/L, boric acid 5.9mg/L, potassium iodide 0.7mg/L, sodium molybdate 0.2mg/L, copper sulphate 0.2mg/L and cobalt oxide 0.03mg/L;
Wherein molysite is ferrous sulfate heptahydrate 26.5mg/L;
Wherein organic matter comprises inositol 100mg/L, glycine 3mg/L, nicotinic acid 0.5mg/L, sucrose 35g/L and nicotinic acid pyridoxine 0.5mg/L;
Wherein in induced bud differential medium, hormone is 2,4-D0.3mg/L and 6BA0.3mg/L; In root induction medium, hormone is NAA0.2mg/L and 6BA0.2mg/L; Expanding hormone in breeding culture medium is 2,4-D0.2mg/L, NAA0.1mg/L and 6BA0.2mg/L.
2. the culture media composition of a kind of Strawberry tissue culture according to claim 1, is characterized in that: described gel is the mixture of agar and gellan gum, and mass ratio is 3:1.
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| CN117305217A (en) * | 2023-10-24 | 2023-12-29 | 临沂市农业科学院 | Optimized stem tip tissue culture medium, culture method and application |
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| CN102893869B (en) * | 2012-10-22 | 2013-10-16 | 浙江省农业科学院 | Root tip detoxification and rapid propagation technology of strawberries |
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Effective date of registration: 20210105 Address after: 225300 No.25 Chunhui Road, Zhangguo Town, Xinghua City, Taizhou City, Jiangsu Province Patentee after: Xinghua Lingyi science and Technology Consulting Service Co.,Ltd. Address before: Room 1406, 14 / F, unit 1, building 5, No. 366, Ronghua South Road, high tech Zone, Chengdu, Sichuan 610000 Patentee before: Sichuan qiyibiao Information Technology Co.,Ltd. Effective date of registration: 20210105 Address after: Room 1406, 14 / F, unit 1, building 5, No. 366, Ronghua South Road, high tech Zone, Chengdu, Sichuan 610000 Patentee after: Sichuan qiyibiao Information Technology Co.,Ltd. Address before: No.112 NInghang Road, Huayang Town, Jurong City, Zhenjiang City, Jiangsu Province Patentee before: ZHENJIANG AGRICULTURAL SCIENCE & TECHNOLOGY INDUSTRIAL Co. |
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Effective date of registration: 20211026 Address after: 750000 group 3, Hongxi village, Hongguang Town, Helan County, Yinchuan City, Ningxia Hui Autonomous Region Patentee after: He Cong Address before: 225300 No.25 Chunhui Road, Zhangguo Town, Xinghua City, Taizhou City, Jiangsu Province Patentee before: Xinghua Lingyi science and Technology Consulting Service Co.,Ltd. |
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