CN103418160B - A kind of extract and extracting method extracting plant polyphenol - Google Patents

A kind of extract and extracting method extracting plant polyphenol Download PDF

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CN103418160B
CN103418160B CN201210154936.0A CN201210154936A CN103418160B CN 103418160 B CN103418160 B CN 103418160B CN 201210154936 A CN201210154936 A CN 201210154936A CN 103418160 B CN103418160 B CN 103418160B
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plant polyphenol
raw material
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CN103418160A (en
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王承明
王炜
肖頔
高洁芬
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Huazhong Agricultural University
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Abstract

The invention belongs to phytochemicals production preparing technical field, specifically disclose a kind of from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat or lichee, extract plant polyphenol extract and extracting method.This extract to be pH be 7.5 ~ 10.0 hydrogen peroxide solution or pH be 7.5 ~ 10.0 liquor natrii hypochloritis pH be 7.5 ~ 10.0 postassium hypochlorite solution or pH be the calcium hypochlorite solution of 7.5 ~ 10.0.After above raw material is pulverized, sieve, then extract with above-mentioned extract, can ultrasonic wave or microwave treatment be carried out in leaching process, remove residue through centrifugation, namely obtain containing plant polyphenol leaching liquor.The plant polyphenol source that the present invention extracts is wide, industrially has good popularizing application prospect.

Description

A kind of extract and extracting method extracting plant polyphenol
Technical field
The invention belongs to technical field of natural product extraction, be specifically related to a kind of from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee, extract plant polyphenol plant polyphenol extract and extracting method, relevant with agricultural product and food processing field.
Background technology
Plant polyphenol (Plant polyphenol) has another name called vegatable tannin (Vegetable tannin), for the complicated phenols secondary metabolites in plant, there is polyhydric phenols structure, mainly be present in the skin of plant, root, leaf, fruit, the content in plant is only second to cellulose, hemicellulose and lignin.Flavonoids in polyphenol substance is antioxidant.Medical research shows, oxidative damage causes many chronic diseases, and as cardiovascular disease, the major reason of cancer and senile disease, the anti-oxidation function of polyphenol can play prevention effect to these chronic diseases.Polyphenol, as a kind of antioxidant, has extraordinary curative effect to angiocardiopathy preventing, and this point obtains Chinese and foreign department scholar experimental demonstration for many years.From the later stage eighties 20th century, both at home and abroad from multiple field, multiple angles carried out basic research and application study to plant polyphenol.
The chemical property that the unique texture of polyphenol has given it some row are unique, make it have antitumor, anti-oxidant, anti arteriosclerosis, prevent and treat the cardiovascular and cerebrovascular disease such as coronary heart disease and apoplexy and the multiple reason function such as antibacterial, serve certain utilization in sides such as food, medicine, cosmetics, household chemicals and health products.Along with the rise gradually of natural products exploitation, plant polyphenol, because it is in features such as the popularity of plant kingdom's distribution, the diversity of physiological function and abundance, is the focus of current research gradually, is called visually " a untapped gold mine ".Up to the present, large quantifier elimination has been done to the extracting method of plant polyphenol and technique both at home and abroad.The extracting method of plant polyphenol mainly contains organic solvent method and water seaoning, and new method derivative on this basis, as water extraction-precipitation method, ultrasonic wave and microwave assisting method etc.But the yield that these methods extract plant polyphenol is not high, and production technology, equipment are comparatively complicated, and cost is high, is difficult to the needs meeting suitability for industrialized production.
Summary of the invention
The object of the invention is to the deficiency making up existing plant polyphenol extractive technique, a kind of extracting method extracting plant polyphenol from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee is provided, the yield that the method extracts plant polyphenol is higher, and method is simple, low for equipment requirements, save cost.
Realizing the technical scheme that the object of the invention adopts is:
An extract from rapeseed meal or rapeseed coat, peanut shells, apples, persimmons, grapes, oranges, carrots, beans, dates, peel, banana peel, sweet potato, hawthorn skin, tomatoes, nuts, oats, litchi plant polyphenol extract, the extract of the sodium hydroxide to a pH of between 7.5 and 10.0, or a pH of between 7.5 and 10.0 hydrogen peroxide solution, sodium hydroxide or sodium hypochlorite solution pH to between 7.5 and 10.0 sodium hydroxide or potassium hypochlorite solution of pH potassium hydroxide or potassium hypochlorite solution of sodium hypochlorite solution between 7.5 and 10.0, or a pH of between 7.5 and 10.0 of the hydrogen peroxide solution of potassium hydroxide or potassium hydroxide at pH between 7.5 and 10.0 sodium hypochlorite solution or a pH of between 7.5 and 10.0 of pH is between 7.5 and 10.0 potassium hydroxide or calcium hypochlorite solution pH of between 7.5 and 10.0 of calcium hydroxide or hydrogen peroxide solution pH of between 7.5 and 10.0 of calcium hydroxide or sodium hypochlorite solution pH of between 7.5 and 10.0 of calcium hydroxide potassium hypochlorite solution or a pH of between 7.5 and 10.0 of calcium hydroxide or calcium hypochlorite solution pH of between 7.5 and 10.0 of the ammonia hydrogen peroxide solution or a pH of between 7.5 and 10.0 ammonia or sodium hypochlorite solution pH of between 7.5 and 10.0 potassium hypochlorite solution of ammonia or a pH between 7.5 and 10.0 ammonia calcium hypochlorite solution or a pH of between 7.5 and 10.0 of added hydrogen peroxide solution, phosphate buffer or a pH of between 7.5 and 10.0 are added with a phosphate buffer solution of sodium hypochlorite or a pH of between 7.5 and 10.0 plus There phosphate buffer solution or potassium hypochlorite is added with a pH of between 7.5 and 10.0 phosphate buffer or calcium hypochlorite solution has a pH of between 7.5 and 10.0 plus borax - calcium chloride buffer solution or a pH of hydrogen peroxide plus there are between 7.5 and 10.0 borax - calcium chloride buffer solution of sodium hypochlorite or a pH of between 7.5 and 10.0 are added with borax - calcium chloride buffer potassium hypochlorite solution or a pH of between 7.5 and 10.0 are added with borax - calcium chloride buffer pH is between 7.5 and 10.0 or calcium hypochlorite solution of ammonia plus ammonium chloride buffer solution of hydrogen peroxide or a pH of between 7.5 and 10.0 are added with ammonia - ammonium chloride buffer solution of sodium hypochlorite or a pH of 7.5 10.0 plus ammonia - ammonium chloride buffer potassium hypochlorite solution or a pH of between 7.5 and 10.0 are added with ammonia - ammonium chloride buffer solution of calcium hypochlorite.
Wherein
Described extract recipe and preparation method are:
(1) in hydrogen peroxide solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain NaOH hydrogen peroxide solution or potassium hydroxide hydrogen peroxide solution or calcium hydroxide hydrogen peroxide solution or ammoniacal liquor hydrogen peroxide solution or be added with the hydrogen peroxide solution of phosphate buffer or be added with the hydrogen peroxide solution of borax-calcium chloride buffer solution or be added with the hydrogen peroxide solution of ammonia-ammonium chloride buffer;
(2) in liquor natrii hypochloritis, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain NaOH liquor natrii hypochloritis or potassium hydroxide liquor natrii hypochloritis or calcium hydroxide liquor natrii hypochloritis or ammoniacal liquor liquor natrii hypochloritis or be added with the liquor natrii hypochloritis of phosphate buffer or be added with the liquor natrii hypochloritis of borax-calcium chloride buffer solution or be added with the liquor natrii hypochloritis of ammonia-ammonium chloride buffer;
(3) in postassium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain NaOH postassium hypochlorite solution or potassium hydroxide postassium hypochlorite solution or calcium hydroxide postassium hypochlorite solution or ammoniacal liquor postassium hypochlorite solution or be added with the postassium hypochlorite solution of phosphate buffer or be added with the postassium hypochlorite solution of borax-calcium chloride buffer solution or be added with the postassium hypochlorite solution of ammonia-ammonium chloride buffer;
(4) in calcium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain NaOH calcium hypochlorite solution or potassium hydroxide calcium hypochlorite solution or calcium hydroxide calcium hypochlorite solution or ammoniacal liquor calcium hypochlorite solution or be added with the calcium hypochlorite solution of phosphate buffer or be added with the calcium hypochlorite solution of borax-calcium chloride buffer solution or be added with the calcium hypochlorite solution of ammonia-ammonium chloride buffer.
Described phosphate-buffered formula of liquid and preparation method are:
(1) NaH is got 2pO 42H 2o or NaH 2pO 4h 2o, adds redistilled water and dissolves to 1000ml, is mixed with the Na of solution 0.2mol/L 2hPO 4solution;
(2) Na is got 2hPO 412H 2o or Na 2hPO 47H 2o or Na 2hPO 42H 2o, adds redistilled water and dissolves to 1000ml, is mixed with the Na of 0.2mol/L 2hPO 4solution;
(3) NaH of the 0.2mol/L prepared by step (2) 2pO 4the Na of solution and 0.2mol/L 2hPO 412H 2o solution fully mixes and obtains phosphate buffer, by adjustment NaH 2pO 4solution and Na 2hPO 412H 2o liquor capacity is than regulating its pH to 7.5-10.0.
Described borax-calcium chloride buffer formulation and preparation method are: get borax, calcium chloride, after the 800ml that adds water dissolves, regulate pH to 7.5-10.0, be diluted with water to 1000ml, obtain borax-calcium chloride buffer solution with 1mol/L hydrochloric acid solution.
Described ammonia chloride buffer formula of liquid and preparation method are: get ammonium chloride, be dissolved in water to 100ml, then add liquor ammoniae dilutus adjustment pH to 7.5-10.0, obtain ammonia-ammonium chloride buffer.
Applicant providing one utilizes above-mentioned plant polyphenol extract of preparing from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, the method of plant polyphenol is extracted in oat or lichee, its concrete steps are: pulverized by above-described raw material, the plant polyphenol extract described in claim 1-5 is added in one of raw material wherein, the mass volume ratio of described raw material and described plant polyphenol extract is 1:15-1:30, stir at 50-70 DEG C, extract 2.5-4.0 hour, residue is removed through centrifugation, namely obtain containing plant polyphenol leaching liquor.
The described extracting method extracting plant polyphenol from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee, it is characterized in that: above-described raw material is pulverized, dry at being placed in 30-60 DEG C, make below its moisture to 5%, then cross 20-100 mesh sieve, obtain the powder of above raw material.
Described hydrogen peroxide is 2.4:1-6:1 with the volume mass ratio of above-described raw material.
Add in above raw material in the process of lixiviate after described plant polyphenol extract, making it be in power is process 0-30min under the ultrasonic wave of 0-10000W or power is process 0-1min under the microwave of 0-80000W.
To obtain containing after plant polyphenol leaching liquor, by its constant volume, then carry out colorimetric at 765nm, quantitative measurment by Folin-phenol colorimetric method.
Compared with prior art, advantage of the present invention is:
Plant polyphenol extract of the present invention is the alkaline hydrogen peroxide aqueous solution, in the basic conditions, plant polyphenol degree of ionization strengthens, solubility is in the solution increased, therefore adopt this plant polyphenol extract and extracting method can fully from the plant polyphenol dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee, yield is higher, method is simple, low for equipment requirements, save cost, be easy to suitability for industrialized production.
The present invention utilizes the method for microwave reinforced solid-liquid leaching to be the novel auxiliary extraction technology of quite potential one in leaching process, its principle is that microwave radiation radiation is in solvent and through cell membrane arrival cell interior, because solvent and cell liquid absorb microwave energy, cell interior temperature raises, and pressure increases.When pressure exceedes the ability to bear of cell membrane, cell wall rupture, the active ingredient being arranged in cell interior discharges from cell, transmits and transfers to around solvent by dissolution with solvents.Microwave assisted Extraction is followed the example of has selective height, and extraction time is short, easily for the extraction yield of volatile ingredient is high and do not need the advantages such as special separating step.
The present invention utilizes the method for intensified by ultrasonic wave solid-liquid leaching to be also the novel auxiliary extraction technology of quite potential one in leaching process, its principle is that hyperacoustic effect of vibration can improve broken speed, shorten the broken time, promote that chemical substance is dissolved in solvent; The effects such as hyperacoustic cavitation effect, stirring simultaneously can strengthen the convection current of leaching liquor, reduce diffusional resistance, impel mass transfer velocity to accelerate, thus expand the aperture of dialysis membrane, make the large molecule solid contents such as polyphenol be more prone to ooze out, be applicable to extracting plant polyphenol.Through the extract of ultrasonic wave process, its structures and characteristics can not change, and extraction time is short, and yield is advantages of higher comparatively.
Detailed description of the invention
Below by specific embodiment, the present invention is further illustrated, but protection content of the present invention is not limited to following examples.
Following methods is adopted to carry out the pretreatment of following steps to dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee in the embodiment of the present invention: to be pulverized by above raw material, dry under being placed in 30-60 DEG C of temperature, its moisture is made to be down to less than 5%, then 20-100 mesh sieve is crossed, obtain the powder of above raw material, for subsequent use.
Prepared by embodiment 1 associated extraction liquid
Prepared by extract:
(1) in hydrogen peroxide solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain NaOH hydrogen peroxide solution or potassium hydroxide hydrogen peroxide solution or calcium hydroxide hydrogen peroxide solution or ammoniacal liquor hydrogen peroxide solution or be added with the hydrogen peroxide solution of phosphate buffer or be added with the hydrogen peroxide solution of borax-calcium chloride buffer solution or be added with the hydrogen peroxide solution of ammonia-ammonium chloride buffer;
(2) in liquor natrii hypochloritis, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain NaOH liquor natrii hypochloritis or potassium hydroxide liquor natrii hypochloritis or calcium hydroxide liquor natrii hypochloritis or ammoniacal liquor liquor natrii hypochloritis or be added with the liquor natrii hypochloritis of phosphate buffer or be added with the liquor natrii hypochloritis of borax-calcium chloride buffer solution or be added with the liquor natrii hypochloritis of ammonia-ammonium chloride buffer;
(3) in postassium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain NaOH postassium hypochlorite solution or potassium hydroxide postassium hypochlorite solution or calcium hydroxide postassium hypochlorite solution or ammoniacal liquor postassium hypochlorite solution or be added with the postassium hypochlorite solution of phosphate buffer or be added with the postassium hypochlorite solution of borax-calcium chloride buffer solution or be added with the postassium hypochlorite solution of ammonia-ammonium chloride buffer;
(4) in calcium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain NaOH calcium hypochlorite solution or potassium hydroxide calcium hypochlorite solution or calcium hydroxide calcium hypochlorite solution or ammoniacal liquor calcium hypochlorite solution or be added with the calcium hypochlorite solution of phosphate buffer or be added with the calcium hypochlorite solution of borax-calcium chloride buffer solution or be added with the calcium hypochlorite solution of ammonia-ammonium chloride buffer.
Wherein
Phosphate-buffered formula of liquid and preparation method are:
(1) NaH is got 2pO 42H 2o or NaH 2pO 4h 2o, adds redistilled water and dissolves to 1000ml, is mixed with the Na of 0.2mol/L 2hPO 4solution;
(2) Na is got 2hPO 412H 2o or Na 2hPO 47H 2o or Na 2hPO 42H 2o, adds redistilled water and dissolves to 1000ml, is mixed with the Na of 0.2mol/L 2hPO 4solution;
(3) NaH of the 0.2mol/L prepared by step (2) 2pO 4the Na of solution and 0.2mol/L 2hPO 412H 2o solution fully mixes and obtains phosphate buffer, by adjustment NaH 2pO 4solution and Na 2hPO 412H 2o liquor capacity, than regulating its pH to 7.5-10.0, saves backup under room temperature.
Described borax calcium chloride buffer formulation and preparation method are: get borax, calcium chloride, after the 800ml that adds water dissolves, regulate pH to 7.5-10.0, be diluted with water to 1000ml, obtain borax-calcium chloride buffer solution with 1mol/L hydrochloric acid solution.
Described-ammonia chloride buffer formula of liquid and preparation method are: get ammonium chloride, be dissolved in water to 100ml, then add liquor ammoniae dilutus and regulate pH to 7.5-10.0, obtain ammonia-ammonium chloride buffer.
Embodiment 2
Get the pretreated rapeseed cake dregs of 1g or rapeseed hull (does not limit rape variety, the method of pretreatment of raw material is: above raw material is pulverized by application conventional grinder, dry at being placed in 30-60 DEG C, its moisture is made to be down to less than 5%, then 20-100 mesh sieve is crossed, obtain the powder of pretreated raw material) powder, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added in raw material wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), lixiviate 3h at 50 DEG C, residue (i.e. remaining raw material after lixiviate) is removed through centrifugation, obtain containing plant polyphenol leaching liquor, analytical method adopts Folin-phenol colorimetric method (Liu Qing etc., the content [J] of total polyphenols in Folin-Ciocalteu colorimetric method for determining barley plants polyphenol extract. food science and technology, 2007, 32 (4): 175-177) in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.57mg/g.
Embodiment 3
The method provided according to enforcement 2 is carried out.Get the pretreated peanut shell powder of 1g, then the hydrogen peroxide solution extract (pH is 7.77) being added with borax calcium chloride buffer solution that embodiment 1 prepares is added wherein, wherein the pH of borax calcium chloride buffer solution is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 55 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.99mg/g.
Embodiment 4
The method provided according to enforcement 2 is carried out.Get the apple powder after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with ammonia ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia ammonium chloride buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 60 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.16mg/g.
Embodiment 5
The method provided according to enforcement 2 is carried out.Get the persimmon powder after 1g pretreatment, then the NaOH hydrogen peroxide solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 6
The method provided according to enforcement 2 is carried out.Get the pretreated grape powder of 1g, then the potassium hydroxide hydrogen peroxide solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(H 2o 2compare for 1.5:1 with the volume mass of raw material), then lixiviate 3h at 70 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 7
The method provided according to enforcement 2 is carried out.Get the citrus powder after 1g pretreatment, then the calcium hydroxide hydrogen peroxide solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 2h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.13mg/g.
Embodiment 8
The method provided according to enforcement 2 is carried out.Get the radish powder after 1g pretreatment, then the ammoniacal liquor hydrogen peroxide solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 2.5h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.77mg/g.
Embodiment 9
The method provided according to enforcement 2 is carried out.Get the powdered soybean after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3.0h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 10
The method provided according to enforcement 2 is carried out.Get the jujube powder after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 10.17mg/g.
Embodiment 11
The method provided according to enforcement 2 is carried out.Get the Pomegranate Rind after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 4.0h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 12
The method provided according to enforcement 2 is carried out.Get the banana skin powder after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 10mL(raw material and plant polyphenol extract is 1:10), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), the compound method of buffer solution used is: get borax 0.572g and calcium chloride 2.94g, after the about 800ml that adds water dissolves, be 7.7 by 1mol/L hydrochloric acid solution adjust ph, be diluted with water to 1000ml, obtain pH=7.7 borax-calcium chloride buffer solution.Then lixiviate 3.5h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 10.77mg/g.
Embodiment 13
The method provided according to enforcement 2 is carried out.Get the sweet potato powder after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 15mL(raw material and plant polyphenol extract is 1:15), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 11.36mg/g.
Embodiment 14
The method provided according to enforcement 2 is carried out.Get the hawthorn skin powder after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 11.42mg/g.
Embodiment 15
The method provided according to enforcement 2 is carried out.Get the tomato powder after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 0min under making it be in the ul-trasonic irradiation of 0W power in leaching process and namely do not carry out ultrasonic wave process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 10.50mg/g.
Embodiment 16
The method provided according to enforcement 2 is carried out.Get the pine nut (powder after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 30mL(raw material and plant polyphenol extract is 1:30), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 15min under making it be in the ul-trasonic irradiation of 0W power in leaching process and namely do not carry out ultrasonic wave process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 10.08mg/g.
Embodiment 17
The method provided according to enforcement 2 is carried out.Get the oat powder after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 0.6mL(hydrogen peroxide is 0.6:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 30min under making it be in the ul-trasonic irradiation of 0W power in leaching process and namely do not carry out ultrasonic wave process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 5.44mg/g.
Embodiment 18
The method provided according to enforcement 2 is carried out.Get the lichee powder after 1g pretreatment, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 0min under making it be in the ul-trasonic irradiation of 5000W power in leaching process and namely do not carry out ultrasonic wave process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Fol in phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 11.42mg/g.
Embodiment 19
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 2.4mL(hydrogen peroxide is 2.4:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 15min is processed under making it be in the ul-trasonic irradiation of 5000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 17.01mg/g.
Embodiment 20
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 3.3mL(hydrogen peroxide is 3.3:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 30min is processed under making it be in the ul-trasonic irradiation of 5000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.60mg/g.
Embodiment 21
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 4.2mL(hydrogen peroxide is 4.2:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 0min under making it be in the ul-trasonic irradiation of 10000W power in leaching process and namely do not carry out ultrasonic wave process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.68mg/g.
Embodiment 22
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), the compound method of buffer solution used is: get ammonium chloride 1.07g, add water and make to be dissolved into 100ml, adding liquor ammoniae dilutus adjust ph is again 7.7, obtain the ammonia-ammonium chloride buffer of pH=7.7.Then lixiviate 3.5h at 65 DEG C, processes 15min under making it be in the ul-trasonic irradiation of 10000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 20.95mg/g.
Embodiment 23
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 6mL(hydrogen peroxide is 6:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 0min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 20.19mg/g.
Embodiment 24
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 6.9mL(hydrogen peroxide is 6.9:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 0.5min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.84mg/g.
Embodiment 25
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.5) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 26
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.5) being added with borax-calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 27
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.5) being added with ammonia ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 28
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the NaOH hydrogen peroxide solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 29
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the potassium hydroxide hydrogen peroxide solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 30
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hydroxide hydrogen peroxide solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 31
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the ammoniacal liquor hydrogen peroxide solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 32
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 8.0) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 8.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 0min under making it be in the microwave action of 40000W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.05mg/g.
Embodiment 33
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 8.5) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 8.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 40000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.53mg/g.
Embodiment 34
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 9.0) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 9.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 40000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 20.64mg/g.
Embodiment 35
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 9.5) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 9.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 0min under making it be in the microwave action of 80000W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.97mg/g.
Embodiment 36
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 10.0) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 37
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 10.0) being added with borax calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 38
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 10.0) being added with ammonia-ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 39
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the NaOH hydrogen peroxide solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 40
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the potassium hydroxide hydrogen peroxide solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 41
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hydroxide hydrogen peroxide solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 42
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the ammoniacal liquor hydrogen peroxide solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 43
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 30min at 65 DEG C, 30min is processed under making it be in the ul-trasonic irradiation of 10000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 28.96mg/g.
Embodiment 44
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 1min at 65 DEG C, processes 1min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 30.34mg/g.
Embodiment 45
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.5) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 46
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 10.0) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 47
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 7.5) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 48
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the hydrogen peroxide solution extract (pH is 10.0) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 49
Get the pretreated rapeseed cake dregs of 1g or rapeseed hull (does not limit rape variety, the method of pretreatment of raw material is: above raw material is pulverized by application conventional grinder, dry at being placed in 30-60 DEG C, its moisture is made to be down to less than 5%, then 20-100 mesh sieve is crossed, obtain the powder of pretreated raw material) powder, then the liquor natrii hypochloritis's extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added in raw material wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), lixiviate 3h at 50 DEG C, residue (i.e. remaining raw material after lixiviate) is removed through centrifugation, obtain containing plant polyphenol leaching liquor, analytical method adopts Folin-phenol colorimetric method (Liu Qing etc., the content [J] of total polyphenols in Folin-Ciocalteu colorimetric method for determining barley plants polyphenol extract. food science and technology, 2007, 32 (4): 175177) in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.57mg/g.
Embodiment 50
The method provided according to enforcement 2 is carried out.Get the pretreated peanut shell powder of 1g, then the liquor natrii hypochloritis's extract (pH is 7.77) being added with borax-calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 55 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.99mg/g.
Embodiment 51
The method provided according to enforcement 2 is carried out.Get the apple powder after 1g pretreatment, then the liquor natrii hypochloritis's extract (pH is 7.77) being added with ammonia ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia ammonium chloride buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 60 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.16mg/g.
Embodiment 52
The method provided according to enforcement 2 is carried out.Get the persimmon powder after 1g pretreatment, then the NaOH liquor natrii hypochloritis extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 53
The method provided according to enforcement 2 is carried out.Get the pretreated grape powder of 1g, then the potassium hydroxide liquor natrii hypochloritis extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 70 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 54
The method provided according to enforcement 2 is carried out.Get the citrus powder after 1g pretreatment, then the calcium hydroxide liquor natrii hypochloritis extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then lixiviate 2h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.13mg/g.
Embodiment 55
The method provided according to enforcement 2 is carried out.Get the radish powder after 1g pretreatment, then the ammoniacal liquor liquor natrii hypochloritis extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then lixiviate 2.5h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.77mg/g.
Embodiment 56
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the liquor natrii hypochloritis's extract (pH is 7.5) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 57
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the liquor natrii hypochloritis's extract (pH is 7.5) being added with borax-calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 58
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the liquor natrii hypochloritis's extract (pH is 7.5) being added with ammonia-ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 59
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the NaOH liquor natrii hypochloritis extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 60
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the potassium hydroxide liquor natrii hypochloritis extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 61
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hydroxide liquor natrii hypochloritis extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 62
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the ammoniacal liquor liquor natrii hypochloritis extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 63
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the liquor natrii hypochloritis's extract (pH is 10.0) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 64
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the liquor natrii hypochloritis's extract (pH is 10.0) being added with borax-calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 65
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the liquor natrii hypochloritis's extract (pH is 10.0) being added with ammonia-ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 66
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the NaOH liquor natrii hypochloritis extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 67
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the potassium hydroxide liquor natrii hypochloritis extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 68
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hydroxide liquor natrii hypochloritis extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 69
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the ammoniacal liquor liquor natrii hypochloritis extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 70
Get the pretreated rapeseed cake dregs of 1g or rapeseed hull (does not limit rape variety, the method of pretreatment of raw material is: above raw material is pulverized by application conventional grinder, dry at being placed in 30-60 DEG C, its moisture is made to be down to less than 5%, then 20-100 mesh sieve is crossed, obtain the powder of pretreated raw material) powder, then the postassium hypochlorite solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added in raw material wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), lixiviate 3h at 50 DEG C, residue (i.e. remaining raw material after lixiviate) is removed through centrifugation, obtain containing plant polyphenol leaching liquor, analytical method adopts Folin-phenol colorimetric method (Liu Qing etc., the content [J] of total polyphenols in Folin-Ciocalteu colorimetric method for determining barley plants polyphenol extract. food science and technology, 2007, 32 (4): 175-177) in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.57mg/g.
Embodiment 71
The method provided according to enforcement 2 is carried out.Get the pretreated peanut shell powder of 1g, then the postassium hypochlorite solution extract (pH is 7.77) being added with borax-calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 55 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.99mg/g.
Embodiment 72
The method provided according to enforcement 2 is carried out.Get the apple powder after 1g pretreatment, then the postassium hypochlorite solution extract (pH is 7.77) being added with ammonia-ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 60 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.16mg/g.
Embodiment 73
The method provided according to enforcement 2 is carried out.Get the persimmon powder after 1g pretreatment, then the NaOH postassium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 74
The method provided according to enforcement 2 is carried out.Get the pretreated grape powder of 1g, then the potassium hydroxide postassium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 70 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 75
The method provided according to enforcement 2 is carried out.Get the citrus powder after 1g pretreatment, then the calcium hydroxide postassium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 2h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.13mg/g.
Embodiment 76
The method provided according to enforcement 2 is carried out.Get the radish powder after 1g pretreatment, then the ammoniacal liquor postassium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 2.5h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.77mg/g.
Embodiment 77
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the postassium hypochlorite solution extract (pH is 7.5) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 78
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the postassium hypochlorite solution extract (pH is 7.5) being added with borax calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 79
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the postassium hypochlorite solution extract (pH is 7.5) being added with ammonia-ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 80
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the NaOH postassium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 81
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the potassium hydroxide postassium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 82
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hydroxide postassium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 83
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the ammoniacal liquor postassium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 84
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the postassium hypochlorite solution extract (pH is 10.0) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 85
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the postassium hypochlorite solution extract (pH is 10.0) being added with borax calcium chloride buffer solution that embodiment 1 prepares is added wherein, wherein the pH of borax calcium chloride buffer solution is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 86
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the postassium hypochlorite solution extract (pH is 10.0) being added with ammonia ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 87
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the NaOH postassium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 88
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the potassium hydroxide postassium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 89
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hydroxide postassium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 90
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the ammoniacal liquor postassium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 91
Get the pretreated rapeseed cake dregs of 1g or rapeseed hull (does not limit rape variety, the method of pretreatment of raw material is: above raw material is pulverized by application conventional grinder, dry at being placed in 30-60 DEG C, its moisture is made to be down to less than 5%, then 20-100 mesh sieve is crossed, obtain the powder of pretreated raw material) powder, then the calcium hypochlorite solution extract (pH is 7.77) being added with phosphate buffer that embodiment 1 prepares is added in raw material wherein, wherein the pH of phosphate buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), lixiviate 3h at 50 DEG C, residue (i.e. remaining raw material after lixiviate) is removed through centrifugation, obtain containing plant polyphenol leaching liquor, analytical method adopts Folin-phenol colorimetric method (Liu Qing etc., the content [J] of total polyphenols in Folin-Ciocalteu colorimetric method for determining barley plants polyphenol extract. food science and technology, 2007, 32 (4): 175-177) in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.57mg/g.
Embodiment 92
The method provided according to enforcement 2 is carried out.Get the pretreated peanut shell powder of 1g, then the calcium hypochlorite solution extract (pH is 7.77) being added with borax-calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 55 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.99mg/g.
Embodiment 93
The method provided according to enforcement 2 is carried out.Get the apple powder after 1g pretreatment, then the calcium hypochlorite solution extract (pH is 7.77) being added with ammonia-ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 7.7, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 60 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.16mg/g.
Embodiment 94
The method provided according to enforcement 2 is carried out.Get the persimmon powder after 1g pretreatment, then the NaOH calcium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 95
The method provided according to enforcement 2 is carried out.Get the pretreated grape powder of 1g, then the potassium hydroxide calcium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 3h at 70 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 96
The method provided according to enforcement 2 is carried out.Get the citrus powder after 1g pretreatment, then the calcium hydroxide calcium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 2h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.13mg/g.
Embodiment 97
The method provided according to enforcement 2 is carried out.Get the radish powder after 1g pretreatment, then the ammoniacal liquor calcium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then lixiviate 2.5h at 65 DEG C.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.77mg/g.
Embodiment 98
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hypochlorite solution extract (pH is 7.5) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 99
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hypochlorite solution extract (pH is 7.5) being added with borax-calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 100
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hypochlorite solution extract (pH is 7.5) being added with ammonia-ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 7.5, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, process 1min under making it be in the microwave action of 0W power in leaching process and namely do not carry out microwave treatment.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 101
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the NaOH calcium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 102
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the potassium hydroxide calcium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 103
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hydroxide calcium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 104
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the ammoniacal liquor calcium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 1min under making it be in the microwave action of 0W power and namely do not carry out microwave treatment in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 105
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hypochlorite solution extract (pH is 10.0) being added with phosphate buffer that embodiment 1 prepares is added wherein, wherein the pH of phosphate buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 106
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hypochlorite solution extract (pH is 10.0) being added with borax-calcium chloride buffer solution that embodiment 1 prepares is added wherein, the pH of wherein borax-calcium chloride buffer solution is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 107
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hypochlorite solution extract (pH is 10.0) being added with ammonia-ammonium chloride buffer that embodiment 1 prepares is added wherein, wherein the pH of ammonia-ammonium chloride buffer is 10.0, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, 0.5min is processed under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 108
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the NaOH calcium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 109
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the potassium hydroxide calcium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 110
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the calcium hydroxide calcium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 111
The method provided according to enforcement 2 is carried out.Get the dregs of rapeseed cake after 1g pretreatment or vegetable seed skin powder, then the ammoniacal liquor calcium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares is added wherein, the volume of plant polyphenol extract used is the mass volume ratio of 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then lixiviate 3.5h at 65 DEG C, processes 0.5min under making it be in the microwave action of 80000W power in leaching process.Remove residue (i.e. remaining raw material after lixiviate) through centrifugation, obtain containing plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.

Claims (1)

1. from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee, extract a method for plant polyphenol, it is characterized in that:
(1) Preparation of extract: extract the pH is 7.5 to 10.0 hydrogen peroxide solution or sodium hydroxide to 10.0 pH 7.5 potassium hydroxide solution or hydrogen peroxide at pH 7.5 to 10.0 of a solution of hydrogen peroxide or calcium hydroxide pH 7.5 to 10.0 ammonia hydrogen peroxide solution or a pH of 7.5 to 10.0 phosphate buffer added with hydrogen peroxide solution or pH 7.5 to 10.0 of added phosphate buffer solution of sodium hypochlorite or pH 7.5 to 10.0 plus phosphate buffer salts or potassium hypochlorite solution of pH 7.5 to 10.0 of added phosphate buffer pH 7.5 or calcium hypochlorite solution is added to 10.0 borax - calcium chloride buffer solution or hydrogen peroxide at pH 7.5 ~ borax added 10.0 - calcium chloride buffer pH 7.5 or sodium hypochlorite solution is added to 10.0 borax - calcium chloride buffer potassium hypochlorite solution or pH 7.5 to 10.0 plus borax - calcium chloride buffer The calcium hypochlorite solution or pH 7.5 to 10.0 plus ammonia - ammonium chloride buffer solution or hydrogen peroxide at pH 7.5 to 10.0 of added ammonia - ammonium chloride buffer solution of sodium hypochlorite or pH 7.5 to 10.0 The added ammonia - ammonium chloride buffer potassium hypochlorite solution or pH 7.5 to 10.0 plus ammonia - ammonium chloride buffer solution of calcium hypochlorite;
Described extract recipe and preparation method are:
1) in hydrogen peroxide solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain NaOH hydrogen peroxide solution or potassium hydroxide hydrogen peroxide solution or calcium hydroxide hydrogen peroxide solution or ammoniacal liquor hydrogen peroxide solution or be added with the hydrogen peroxide solution of phosphate buffer or be added with the hydrogen peroxide solution of borax-calcium chloride buffer solution or be added with the hydrogen peroxide solution of ammonia-ammonium chloride buffer;
2) in liquor natrii hypochloritis, add phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain being added with the liquor natrii hypochloritis of phosphate buffer or be added with the liquor natrii hypochloritis of borax-calcium chloride buffer solution or be added with the liquor natrii hypochloritis of ammonia-ammonium chloride buffer;
3) in postassium hypochlorite solution, add phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain being added with the postassium hypochlorite solution of phosphate buffer or be added with the postassium hypochlorite solution of borax-calcium chloride buffer solution or be added with the postassium hypochlorite solution of ammonia-ammonium chloride buffer;
4) in calcium hypochlorite solution, add phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer obtain being added with the calcium hypochlorite solution of phosphate buffer or be added with the calcium hypochlorite solution of borax-calcium chloride buffer solution or be added with the calcium hypochlorite solution of ammonia-ammonium chloride buffer;
Described phosphate-buffered formula of liquid and preparation method are:
1) NaH is got 2pO 42H 2o or NaH 2pO 4h 2o, adds redistilled water and dissolves to 1000ml, is mixed with the NaH of solution 0.2mol/L 2pO 4solution;
2) Na is got 2hPO 412H 2o or Na 2hPO 47H 2o or Na 2hPO 42H 2o, adds redistilled water and dissolves to 1000ml, is mixed with the Na of 0.2mol/L 2hPO 4solution;
3) by the NaH of the 0.2mol/L of preparation 2pO 4the Na of solution and 0.2mol/L 2hPO 4solution fully mixes and obtains phosphate buffer, by adjustment NaH 2pO 4solution and Na 2hPO 4liquor capacity is than regulating its pH to 7.5 ~ 10.0;
Wherein, described borax-calcium chloride buffer formulation and preparation method are: get borax, calcium chloride, after the 800ml that adds water dissolves, regulate pH to 7.5 ~ 10.0, be diluted with water to 1000ml, obtain borax-calcium chloride buffer solution with 1mol/L hydrochloric acid solution;
Described ammonia-chloride buffer formula of liquid and preparation method are: get ammonium chloride, be dissolved in water to 100ml, then add liquor ammoniae dilutus adjustment pH to 7.5 ~ 10.0, obtain ammonia-ammonium chloride buffer;
(2) plant polyphenol is extracted: the extract described in utilization is from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, plant polyphenol is extracted in oat or lichee, above-described raw material is pulverized maybe by the raw material after pulverizing, dry at being placed in 30 ~ 60 DEG C, make below its moisture to 5%, then 20 ~ 100 mesh sieves are crossed, obtain the powder of raw material, described extract is added in one of raw material wherein, described raw material and the mass volume ratio of extract are 1:15 ~ 1:30, stir at 50 ~ 70 DEG C, extract 2.5 ~ 4.0 hours, residue is removed through centrifugation, namely the leaching liquor containing plant polyphenol is obtained, or add in the process of lixiviate after described extract in above raw material, making it be in power is that under the ultrasonic wave of 0 ~ 10000W, process 0 ~ 30min or power are process 0 ~ 1min under the microwave of 0 ~ 80000W,
Wherein
The volume mass of the hydrogen peroxide in described extract or clorox or postassium hypochlorite or calcium hypochlorite and described raw material is than being 2.4:1 ~ 6:1.
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CN1121926A (en) * 1994-10-22 1996-05-08 王崴 Process for extraction of plant protein and use thereof
CN1431231A (en) * 2003-01-29 2003-07-23 广西壮族自治区中国科学院广西植物研究所 Method for extracting xylan from woody fiber material
CN1823856A (en) * 2005-12-24 2006-08-30 安徽乳泉石榴酒业有限公司 Method of extracting polyphenol from pomegranate seed

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1121926A (en) * 1994-10-22 1996-05-08 王崴 Process for extraction of plant protein and use thereof
CN1431231A (en) * 2003-01-29 2003-07-23 广西壮族自治区中国科学院广西植物研究所 Method for extracting xylan from woody fiber material
CN1823856A (en) * 2005-12-24 2006-08-30 安徽乳泉石榴酒业有限公司 Method of extracting polyphenol from pomegranate seed

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