CN111195268A - Method for extracting polyphenols from pomegranate peel - Google Patents
Method for extracting polyphenols from pomegranate peel Download PDFInfo
- Publication number
- CN111195268A CN111195268A CN201811371170.5A CN201811371170A CN111195268A CN 111195268 A CN111195268 A CN 111195268A CN 201811371170 A CN201811371170 A CN 201811371170A CN 111195268 A CN111195268 A CN 111195268A
- Authority
- CN
- China
- Prior art keywords
- pomegranate
- extracting
- polyphenol
- steps
- following
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 71
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 70
- 241000219991 Lythraceae Species 0.000 title claims abstract description 57
- 235000014360 Punica granatum Nutrition 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims abstract description 32
- 241000283690 Bos taurus Species 0.000 claims abstract description 21
- 238000000605 extraction Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000000287 crude extract Substances 0.000 claims abstract description 16
- 239000011347 resin Substances 0.000 claims abstract description 14
- 229920005989 resin Polymers 0.000 claims abstract description 14
- 239000002904 solvent Substances 0.000 claims abstract description 4
- 238000004108 freeze drying Methods 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 238000000746 purification Methods 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 7
- 239000003480 eluent Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000000874 microwave-assisted extraction Methods 0.000 claims description 3
- 239000011344 liquid material Substances 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 238000007873 sieving Methods 0.000 description 9
- 238000004140 cleaning Methods 0.000 description 3
- 230000006837 decompression Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 238000009777 vacuum freeze-drying Methods 0.000 description 3
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Chinese gallotannin Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 1
- JMGCAHRKIVCLFW-UHFFFAOYSA-N 1-O-Galloylcastalagin Natural products Oc1cc(cc(O)c1O)C(=O)OC2C3OC(=O)c4c2c(O)c(O)c(O)c4c5c(O)c(O)c(O)c6c5C(=O)OC3C7OC(=O)c8cc(O)c(O)c(O)c8c9c(O)c(O)c(O)cc9C(=O)OCC7OC(=O)c%10cc(O)c(O)c(O)c6%10 JMGCAHRKIVCLFW-UHFFFAOYSA-N 0.000 description 1
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 1
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 description 1
- 229920002079 Ellagic acid Polymers 0.000 description 1
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 description 1
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 description 1
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 1
- 229940074393 chlorogenic acid Drugs 0.000 description 1
- 235000001368 chlorogenic acid Nutrition 0.000 description 1
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 229960002852 ellagic acid Drugs 0.000 description 1
- 235000004132 ellagic acid Nutrition 0.000 description 1
- JMGCAHRKIVCLFW-CNWXVVPTSA-N ellagitannin Chemical compound OC1=C(O)C(O)=CC(C(=O)O[C@H]2C3=C4C(=O)O[C@@H]2[C@@H]2[C@@H]5OC(=O)C6=CC(O)=C(O)C(O)=C6C6=C(O)C(O)=C(O)C=C6C(=O)OC[C@H]5OC(=O)C5=CC(O)=C(O)C(O)=C5C=5C(O)=C(O)C(O)=C(C=5C(=O)O2)C4=C(O)C(O)=C3O)=C1 JMGCAHRKIVCLFW-CNWXVVPTSA-N 0.000 description 1
- 229920001968 ellagitannin Polymers 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 1
- 235000001785 ferulic acid Nutrition 0.000 description 1
- 229940114124 ferulic acid Drugs 0.000 description 1
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 229920002824 gallotannin Polymers 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Medical Informatics (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Nutrition Science (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Alternative & Traditional Medicine (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to the field of plant extraction, and particularly relates to a method for extracting polyphenols from pomegranate rind. Specifically, the method comprises the steps of taking water as a solvent, extracting by microwave to obtain a crude extract of pomegranate peel polyphenol, separating by macroporous resin, concentrating under reduced pressure, and freeze-drying in vacuum to obtain the pomegranate peel polyphenol. The obtained crude extract of pomegranate peel polyphenol has high total polyphenol content with pomegranate peel polyphenol, the yield of the crude extract reaches 40-50%, and a new choice is provided for obtaining pomegranate peel polyphenol.
Description
Technical Field
The invention belongs to the field of plant extraction, and particularly relates to a method for extracting polyphenols from pomegranate rind.
Background
Pomegranate (punicagrantum) polyphenol [1] is a general name of compounds with polyphenol hydroxyl, comprises ellagitannin, gallotannin, ellagic acid, gallic acid, catechin, anthocyanidin, chlorogenic acid, ferulic acid, quercetin and other compounds, is mainly contained in pomegranate peel, has the content of about 10-20% of the dry mass, and has various physiological and pharmacological activities of oxidation resistance, aging resistance, cancer prevention, antibiosis, skin moistening, beauty treatment, blood pressure reduction, cardiovascular and cerebrovascular disease prevention and the like. Pomegranate polyphenol shows great application value in the fields of food, medicine, daily chemicals and the like.
At present, most of methods for extracting pomegranate polyphenol and utilizing pomegranate peel are decoction methods. The method has the advantages of simple operation and low cost, and is easy to destroy polyphenols and other antioxidant components in pericarpium Granati, and the extractive solution has high impurity content, and is not beneficial to separation and purification of polyphenols. The decoction method mostly uses an organic solvent as an extraction medium, and no relevant report that water is used as the extraction medium is found.
Disclosure of Invention
The invention aims to provide a brand-new method for extracting polyphenols from pomegranate peel, and the method has the advantages of simple and convenient operation and high extraction efficiency.
The invention relates to a method for extracting polyphenol substances in pomegranate peel, which is characterized by comprising the following steps: using water as a solvent, extracting by microwave to obtain a crude extract of pomegranate peel polyphenol, separating by macroporous resin, concentrating under reduced pressure, and freeze-drying in vacuum to obtain the pomegranate peel polyphenol. Wherein, the total polyphenol content in the crude extract of the pomegranate peel polyphenol is 30 to 55 percent; the obtained pericarpium Granati polyphenol has total polyphenol content of 65-80%.
Specifically, the method for extracting polyphenols from pomegranate rind comprises the following steps:
A. crushing pomegranate rind; preferably, the pomegranate rind is crushed to be larger than 40 meshes, and further preferably crushed to be 80-100 meshes;
B. extraction: extracting with water by microwave, and drying the extractive solution to obtain pericarpium Granati polyphenol crude extract; wherein, the microwave
The extraction conditions are as follows: the microwave power is 200-; the preferable microwave extraction conditions are as follows: the temperature is 75 ℃, the liquid-material ratio is 30:1, and the extraction time is 140S;
C. and (3) purification: adding water into the crude extract of pericarpium Granati polyphenol to obtain 4-12mg/mL solution, and passing through D101, D140 or AB-8 macroporous resin at a speed of 0.5-5.5 BV/h; washing with water with the use amount of more than 5 BV; eluting with ethanol with the dosage of 1-6.5BV and the concentration of more than 60% v/v; collecting the eluent, and drying to obtain pomegranate peel polyphenol;
further, the preferred purification conditions are: adding water into the crude extract of pomegranate peel polyphenol to prepare a solution of 10 mg/mL; the macroporous resin is D101 macroporous resin; the speed of the solution prepared by adding water into the crude extract of the pomegranate peel polyphenol to pass through the macroporous resin is 2 BV/h; washing with 5BV water; eluting with ethanol with concentration of more than 70% v/v at 5.5 BV.
In conclusion, the extraction method of the invention uses water as a solvent, and microwave is utilized to effectively extract pomegranate peel polyphenol from pomegranate peel, the total polyphenol content of the obtained crude extract of pomegranate peel polyphenol and the pomegranate peel polyphenol is higher, the yield of the crude extract reaches 40-50%, and a new choice is provided for obtaining the pomegranate peel polyphenol.
Example (b):
example 1
1. Pulverizing and sieving
Taking pomegranate rind, drying, crushing, sieving and sieving by 80 meshes;
2. crude polyphenol extraction
And (3) performing auxiliary extraction by adopting a microwave extractor, wherein the optimized extraction parameters are as follows: extracting with 80-100 mesh pericarpium Granati powder at 75 deg.C for 140S at a liquid-to-material ratio of 30:1 to obtain a total polyphenol content of 22.06%, and vacuum concentrating and lyophilizing to obtain a total polyphenol content of 49.31%;
3. separating and purifying
Adopting D101 macroporous resin with the speed of 2BV/h, the column concentration of 10mg/mL, the cleaning water of 5BV and 70% ethanol eluent with the use amount of 5.5BV to obtain pomegranate peel polyphenol, and obtaining the pomegranate peel polyphenol after decompression concentration and vacuum freeze-drying, wherein the total polyphenol content is 71.64%.
Example 2
1. Pulverizing and sieving
Taking pomegranate rind, drying, crushing, sieving and sieving by a 100-mesh sieve;
2. crude polyphenol extraction
And (3) performing auxiliary extraction by adopting a microwave extractor, wherein the optimized extraction parameters are as follows: extracting with 100 mesh pericarpium Granati powder at 70 deg.C for 150S at a liquid-to-material ratio of 35:1 to obtain a total polyphenol content of 21.20%, and vacuum concentrating and lyophilizing to obtain a total polyphenol content of 48.95%;
3. separating and purifying
Adopting D101 macroporous resin with the speed of 2.5BV/h, the column inlet concentration of 11mg/mL, the cleaning water of 6BV and 70% ethanol eluent with the use amount of 5BV to obtain pomegranate peel polyphenol, and obtaining the pomegranate peel polyphenol after decompression concentration and vacuum freeze-drying, wherein the total polyphenol content is 70.05%.
Example 3
1. Pulverizing and sieving
Taking pomegranate rind, drying, crushing, sieving and sieving by 90 meshes;
2. crude polyphenol extraction
And (3) performing auxiliary extraction by adopting a microwave extractor, wherein the optimized extraction parameters are as follows: extracting with 90 mesh pericarpium Granati powder at 80 deg.C for 120S at a liquid-to-material ratio of 30:1 to obtain a total polyphenol content of 22.03%, and vacuum concentrating and lyophilizing to obtain a total polyphenol content of 50.06%;
3. separating and purifying
Adopting D101 macroporous resin with the speed of 3BV/h, the column concentration of 9mg/mL, the cleaning water of 4.5BV and 70% ethanol eluent with the use amount of 4.5BV to obtain pomegranate peel polyphenol, and obtaining the pomegranate peel polyphenol after decompression concentration and vacuum freeze-drying, wherein the total polyphenol content is 72.18%.
Claims (10)
1. A method for extracting polyphenols from pomegranate rind is characterized by comprising the following steps: using water as a solvent, extracting by microwave to obtain a crude extract of pomegranate peel polyphenol, separating by macroporous resin, concentrating under reduced pressure, and freeze-drying in vacuum to obtain the pomegranate peel polyphenol.
2. The method for extracting polyphenols from pomegranate rind according to claim 1, wherein the method comprises the following steps:
A. crushing pomegranate rind;
B. extraction: extracting with water by microwave, and drying the extractive solution to obtain pericarpium Granati polyphenol crude extract;
wherein, the microwave extraction conditions are as follows: extracting at 60-95 deg.C for 60-180S at a liquid-to-material ratio of 15:1-40: 1;
C. and (3) purification: adding water into the crude extract of pericarpium Granati polyphenol to obtain 4-12mg/mL solution, and passing through D101, D140 or AB-8 macroporous resin at a speed of 0.5-5.5 BV/h; washing with water with the use amount of more than 5 BV; eluting with ethanol with the dosage of 1-6.5BV and the concentration of more than 60% v/v; collecting the eluent, and drying to obtain pomegranate peel polyphenol.
3. The method for extracting polyphenols from pomegranate rind according to claim 2, wherein the method comprises the following steps: the preferable microwave extraction conditions in the step B are as follows: the temperature is 75 ℃, the liquid-material ratio is 30:1, and the extraction time is 140S.
4. The method for extracting polyphenols from pomegranate rind according to claim 2, wherein the method comprises the following steps: in the purification conditions of step C: the crude extract of pomegranate peel polyphenol is added with water to prepare a solution of 10 mg/mL.
5. The method for extracting polyphenols from pomegranate rind according to claim 2, wherein the method comprises the following steps: in the purification conditions of step C: the macroporous resin adopts D101 macroporous resin.
6. The method for extracting polyphenols from pomegranate rind according to claim 2, wherein the method comprises the following steps: in the purification conditions of step C: the speed of the solution prepared by adding water into the crude extract of the pomegranate peel polyphenol to the macroporous resin is 2 BV/h.
7. The method for extracting polyphenols from pomegranate rind according to claim 2, wherein the method comprises the following steps: in the purification conditions of step C: the washing is carried out with water in an amount of 5 BV.
8. The method for extracting polyphenols from pomegranate rind according to claim 2, wherein the method comprises the following steps: in the purification conditions of step C: eluting with ethanol with concentration of more than 70% v/v at 5.5 BV.
9. The method for extracting polyphenols from pomegranate rind according to claim 2, wherein the method comprises the following steps: and step A, crushing the pomegranate rind to the granularity of more than 40 meshes.
10. The method for extracting polyphenols from pomegranate rind according to claim 1, wherein the method comprises the following steps: and step A, crushing the pomegranate rind to 80-100 meshes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811371170.5A CN111195268A (en) | 2018-11-18 | 2018-11-18 | Method for extracting polyphenols from pomegranate peel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811371170.5A CN111195268A (en) | 2018-11-18 | 2018-11-18 | Method for extracting polyphenols from pomegranate peel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111195268A true CN111195268A (en) | 2020-05-26 |
Family
ID=70741275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811371170.5A Withdrawn CN111195268A (en) | 2018-11-18 | 2018-11-18 | Method for extracting polyphenols from pomegranate peel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111195268A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113717139A (en) * | 2021-09-15 | 2021-11-30 | 耒阳市刘燕酿制生物科技有限公司 | Method for extracting quercetin from pomegranate peel |
| CN115918819A (en) * | 2022-12-02 | 2023-04-07 | 广东省农业科学院蚕业与农产品加工研究所 | Pretreatment method for processing soft-seed pomegranate |
-
2018
- 2018-11-18 CN CN201811371170.5A patent/CN111195268A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113717139A (en) * | 2021-09-15 | 2021-11-30 | 耒阳市刘燕酿制生物科技有限公司 | Method for extracting quercetin from pomegranate peel |
| CN115918819A (en) * | 2022-12-02 | 2023-04-07 | 广东省农业科学院蚕业与农产品加工研究所 | Pretreatment method for processing soft-seed pomegranate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101773593B (en) | Method for preparing antioxidative active extractive of sweet potato leaves | |
| CN101744886B (en) | Method for extracting high-purity schisandra total lignan | |
| CN103772473A (en) | Vacuum pulse type method of extracting and purifying ursolic acid of rosemary | |
| CN101301319A (en) | Preparation of granada flower polyphenol and uses thereof | |
| CN101481372B (en) | Process for extracting and purifying tannin and procyanidine in pomegranate peel and seeds | |
| CN104817527A (en) | Method for extracting anthocyanidin from pitaya peel | |
| CN103432562A (en) | Method for extracting fresh ginger polyphenol from fresh ginger | |
| CN105616465A (en) | Method for extracting polyphenol from mango skins | |
| CN104557838A (en) | Method for extracting proantho cyanidins from litchi peels | |
| CN103751317A (en) | Method for extracting hovenia dulcis thunb total flavones by cooperation of surfactant and microwave-ultrasonic extraction process | |
| CN111195268A (en) | Method for extracting polyphenols from pomegranate peel | |
| CN103211051A (en) | Mulberry extract, production method and application thereof | |
| CN106977559A (en) | A kind of method of separating-purifying punicalagins and gallic acid simultaneously from granatum | |
| CN104257882A (en) | Method for simultaneously extracting and separating oil, chlorogenic acid and total flavonoids from Chinese wolfberry residues | |
| CN107573438A (en) | A kind of method that polysaccharide is extracted in the pericarp from passion fruit | |
| CN108069930B (en) | Method for combined extraction of galangal volatile oil and galangin from galangal | |
| CN102031116A (en) | New method for preparing rosemary natural antioxidant | |
| CN104306758A (en) | Method for extracting polyphenol from sugar cane bark | |
| CN105232731A (en) | Method for extracting total flavonoids from litchi shells | |
| CN102327325A (en) | Method for preparing white mulberry rootbark general flavone | |
| CN107183710B (en) | Method for extracting and purifying walnut green husk polyphenol by acidic electrolyzed water | |
| CN112707881B (en) | Preparation method of blueberry anthocyanin | |
| CN104086615A (en) | Method for preparing mogroside V from siraitia grosvenori | |
| CN104800272A (en) | Novel momordica saponin extraction technology | |
| CN112390847A (en) | Method for extracting toosendanin from toosendan fruit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200526 |