CN103418160A - Extracting liquid for extracting plant polyphenol and extracting method - Google Patents

Extracting liquid for extracting plant polyphenol and extracting method Download PDF

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CN103418160A
CN103418160A CN2012101549360A CN201210154936A CN103418160A CN 103418160 A CN103418160 A CN 103418160A CN 2012101549360 A CN2012101549360 A CN 2012101549360A CN 201210154936 A CN201210154936 A CN 201210154936A CN 103418160 A CN103418160 A CN 103418160A
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solution
plant polyphenol
raw material
volume
calcium
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CN103418160B (en
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王承明
王炜
肖頔
高洁芬
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Huazhong Agricultural University
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Huazhong Agricultural University
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Abstract

The invention belongs to the technology field of plant natural product preparation, and specifically discloses a extracting liquid for extracting plant polyphenol from rapeseed cake or rapeseed peel, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, pomegranate peel, banana peel, sweet potato, haw peel, tomato, nut, oat or lichee and a extracting method. The extracting liquid is a hydrogen peroxide solution with a pH value of 7.5 to 10.0, a sodium hypochlorite solution with a pH value of 7.5 to 10.0, a potassium hypochlorite solution with a pH value of 7.5 to 10.0, or a calcium hypochlorite solution with a pH value of 7.5 to 10.0. The preparation method comprises following steps: grinding the raw materials mentioned above, sieving, extracting with the extracting liquid mentioned above, optionally carrying out a supersonic treatment or a microwave treatment during the extracting process, and removing the slag through a centrifugal separation treatment so as to obtain the plant polyphenol leaching liquid. The extracting liquid for extracting plant polyphenol and the extracting method has the advantages of wide plant polyphenol sources and a very good industrial promotion and application prospect.

Description

A kind of extract and extracting method of extracting plant polyphenol
Technical field
The invention belongs to the natural product extracting technique field, be specifically related to a kind of plant polyphenol extract and extracting method of extracting plant polyphenol from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee, relevant with agricultural product and food processing field.
Background technology
Plant polyphenol (Plant polyphenol) has another name called vegatable tannin (Vegetable tannin), for the complicated phenols secondary metabolites in plant, there is the polyhydric phenols structure, mainly be present in skin, root, leaf, the fruit of plant, the content in plant is only second to cellulose, hemicellulose and lignin.Flavonoids in polyphenol substance is antioxidant.Medical research shows, oxidative damage is to cause many chronic diseases, as cardiovascular disease, and the major reason of cancer and senile disease, the anti-oxidation function of polyphenol can play prevention effect to these chronic diseases.Polyphenol, as a kind of antioxidant, has extraordinary curative effect to angiocardiopathy preventing, and this point has obtained Chinese and foreign department scholar experimental demonstration for many years.Since the later stage eighties 20th century, both at home and abroad from a plurality of fields, multiple angles carried out basic research and application study to plant polyphenol.
The unique texture of polyphenol has given it, and some are listed as unique chemical property, make it there is antitumor, anti-oxidant, anti arteriosclerosis, prevent and treat the cardiovascular and cerebrovascular diseases such as coronary heart disease and apoplexy and the multiple reason function such as antibiotic, in sides such as food, medicine, cosmetics, household chemicals and health products, played certain utilization.Along with the rise gradually of natural products exploitation, plant polyphenol, because of the diversity of its popularity distributed in plant kingdom, physiological function and the characteristics such as rich of originating, is the focus of current research gradually, is called visually " a untapped gold mine ".Up to the present, extracting method and the technique of plant polyphenol have been done to a large amount of research both at home and abroad.The extracting method of plant polyphenol mainly contains organic solvent method and water seaoning, and derivative new method on this basis, as water extraction-precipitation method, ultrasonic wave and microwave assisting method etc.But it is not high that these methods are extracted the yield of plant polyphenols, and production technology, equipment are comparatively complicated, and cost is high, is difficult to meet the needs of suitability for industrialized production.
Summary of the invention
The object of the invention is to make up the deficiency of existing plant polyphenol extractive technique, a kind of extracting method of extracting plant polyphenol from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee is provided, the yield that the method is extracted plant polyphenol is higher, and method is simple, low for equipment requirements, save cost.
Realize that the technical scheme that the object of the invention adopts is:
An extract from the skin rapeseed meal or rapeseed, peanut shells, apples, persimmons, grapes, oranges, carrots, beans, dates, peel, banana peel, sweet potato, hawthorn skin, tomatoes, nuts, oats, litchi polyphenol extract, extract the hydrogen peroxide solution of sodium hydroxide to a pH of 7.5-10.0, the pH is sodium hydroxide or sodium hypochlorite solution 7.5-10.0, and sodium hydroxide or potassium hypochlorite solution, pH of 7.5-10.0, sodium or calcium hypochlorite solution to pH of 7.5-10.0, or pH 7.5-10.0 for the hydrogen peroxide solution of potassium hydroxide, potassium hydroxide or sodium hypochlorite solution of pH 7.5-10.0, or to a pH of 7.5-10.0 hydrogen hydroxide solution, potassium hypochlorite, calcium hypochlorite or potassium hydroxide solution, pH 7.5-10.0, or to a pH of 7.5-10.0 calcium hydroxide solution of hydrogen peroxide, calcium hydroxide, or pH 7.5-10.0 sodium hypochlorite solution, the pH, or calcium hydroxide solution 7.5-10.0 potassium hypochlorite or calcium hydroxide to pH 7.5-10.0 solution of calcium hypochlorite, hydrogen peroxide or aqueous ammonia solution at pH 7.5-10.0, and sodium hypochlorite solution or aqueous ammonia to pH 7.5-10.0, and pH of 7.5-10.0 ammonia or potassium hypochlorite solutionCalcium hypochlorite or aqueous ammonia solution pH of 7.5-10.0, 7.5-10.0, or pH of the hydrogen peroxide solution was added with phosphate buffer, pH of 7.5-10.0, or sodium hypochlorite solution was added with a phosphate buffer or pH there is 7.5-10.0 potassium hypochlorite solution plus phosphate buffer, pH of 7.5-10.0, or hypochlorite solution added with a phosphate buffer or a pH of 7.5-10.0 plus borax - calcium chloride buffer solution of hydrogen peroxide, or a pH of 7.5-10.0 plus borax - sodium chloride buffer solution, pH of 7.5-10.0, or plus borax - potassium hypochlorite solution of calcium chloride buffer or a pH of 7.5-10.0 plus borax - calcium hypochlorite, calcium chloride buffer solution, pH of 7.5-10.0, or a hydrogen peroxide solution added with ammonium chloride buffer solution, pH of 7.5-10.0 plus or ammonia - Sodium hypochlorite solution of ammonium chloride buffer, pH of 7.5-10.0 plus or ammonia - ammonium chloride buffer solution, potassium hypochlorite, or to a pH of 7.5-10.0 plus ammonia - ammonium chloride buffer hypochlorous calcium solution.
Wherein
Described extract recipe and preparation method are:
(1) in hydrogen peroxide solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH hydrogen peroxide solution or potassium hydroxide hydrogen peroxide solution or calcium hydroxide hydrogen peroxide solution or ammoniacal liquor hydrogen peroxide solution or be added with the hydrogen peroxide solution of phosphate buffer or be added with the hydrogen peroxide solution of borax-calcium chloride buffer solution or be added with the hydrogen peroxide solution of ammonia-ammonium chloride buffer;
(2) in the liquor natrii hypochloritis, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH liquor natrii hypochloritis or potassium hydroxide liquor natrii hypochloritis or calcium hydroxide liquor natrii hypochloritis or ammoniacal liquor liquor natrii hypochloritis or be added with the liquor natrii hypochloritis of phosphate buffer or be added with the liquor natrii hypochloritis of borax-calcium chloride buffer solution or be added with the liquor natrii hypochloritis of ammonia-ammonium chloride buffer;
(3) in postassium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH postassium hypochlorite solution or potassium hydroxide postassium hypochlorite solution or calcium hydroxide postassium hypochlorite solution or ammoniacal liquor postassium hypochlorite solution or be added with the postassium hypochlorite solution of phosphate buffer or be added with the postassium hypochlorite solution of borax-calcium chloride buffer solution or be added with the postassium hypochlorite solution of ammonia-ammonium chloride buffer;
(4) in calcium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH calcium hypochlorite solution or potassium hydroxide calcium hypochlorite solution or calcium hydroxide calcium hypochlorite solution or ammoniacal liquor calcium hypochlorite solution or be added with the calcium hypochlorite solution of phosphate buffer or be added with the calcium hypochlorite solution of borax-calcium chloride buffer solution or be added with the calcium hypochlorite solution of ammonia-ammonium chloride buffer.
Described phosphate buffered formula of liquid and preparation method are:
(1) get NaH 2PO 42H 2O or NaH 2PO 4H 2O, add redistilled water and dissolve to 1000ml, is mixed with the Na of solution 0.2mol/L 2HPO 4Solution;
(2) get Na 2HPO 412H 2O or Na 2HPO 47H 2O or Na 2HPO 42H 2O, add redistilled water and dissolve to 1000ml, is mixed with the Na of 0.2mol/L 2HPO 4Solution;
(3) NaH of the 0.2mol/L prepared by step (2) 2PO 4The Na of solution and 0.2mol/L 2HPO 412H 2O solution fully mixes and obtains phosphate buffer, by adjusting NaH 2PO 4Solution and Na 2HPO 412H 2The O liquor capacity is than regulating its pH to 7.5-10.0.
Described borax-calcium chloride buffer formulation and preparation method are: get borax, calcium chloride, after adding water 800ml dissolving, regulate pH to 7.5-10.0 with the 1mol/L hydrochloric acid solution, is diluted with water to 1000ml, obtains borax-calcium chloride buffer solution.
Described ammonia chloride buffer formula of liquid and preparation method are: get ammonium chloride, be dissolved in water to 100ml, then add liquor ammoniae dilutus adjusting pH to 7.5-10.0, obtain ammonia-ammonium chloride buffer.
The applicant provides a kind of and has utilized the above-mentioned plant polyphenol extract for preparing from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, the hawthorn skin, tomato, nut, extract the method for plant polyphenol in oat or lichee, its concrete steps are: above-described raw material is pulverized, add the described plant polyphenol extract of claim 1-5 in one of raw material wherein, the mass volume ratio of described raw material and described plant polyphenol extract is 1:15-1:30, under 50-70 ℃, stir, extract 2.5-4.0 hour, remove residue through centrifugation, obtain containing the plant polyphenol leaching liquor.
The described extracting method of extracting plant polyphenol from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee, it is characterized in that: above-described raw material is pulverized, be placed under 30-60 ℃ dry, make below its moisture to 5%, then cross the 20-100 mesh sieve, obtain the powder of above raw material.
Described hydrogen peroxide is 2.4:1-6:1 with the volume mass ratio of above-described raw material.
Add in above raw material in the process of lixiviate after described plant polyphenol extract, make under its ultrasonic wave that is 0-10000W in power to process under the microwave that 0-30min or power are 0-80000W and process 0-1min.
After obtaining containing the plant polyphenol leaching liquor, by its constant volume, then by Folin-phenol colorimetric method, at 765nm, carry out colorimetric, quantitative measurment.
Compared with prior art, advantage of the present invention is:
Plant polyphenol extract of the present invention is the alkaline hydrogen peroxide aqueous solution, under alkali condition, the plant polyphenol degree of ionization strengthens, make the solubility in solution increase, therefore adopt this plant polyphenol extract and extracting method can be fully plant polyphenol from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee, yield is higher, method is simple, low for equipment requirements, save cost, be easy to suitability for industrialized production.
It is a kind of novel auxiliary extraction technology that has much development potentiality that the present invention utilizes the method for microwave reinforced solid-liquid leaching in leaching process, its principle is that the microwave radiation radiation arrives cell interior in solvent and through cell membrane, due to solvent and cell liquid absorption microwave energy, the cell interior temperature raises, and pressure increases.When pressure surpasses the ability to bear of cell membrane, cell wall rupture, the active ingredient that is arranged in cell interior discharges from cell, transmits and transfers to solvent on every side by dissolution with solvents.The microwave assisted Extraction is followed the example of has selective height, and extraction time is short, easily high for the extraction yield of volatile ingredient and do not need the special advantages such as separating step.
It is also a kind of novel auxiliary extraction technology that has much development potentiality that the present invention utilizes the method for intensified by ultrasonic wave solid-liquid leaching in leaching process, its principle is that hyperacoustic effect of vibration can improve broken speed, shorten the broken time, promote chemical substance to dissolve in solvent; The effects such as hyperacoustic cavitation effect, stirring simultaneously can be strengthened the convection current of leaching liquor, reduce diffusional resistance, impel mass transfer velocity to accelerate, thereby enlarge the aperture of dialysis membrane, make the large molecule solid content such as polyphenol be more prone to ooze out, be applicable to plant polyphenol is extracted.The extract of processing through ultrasonic wave, its structure and character can not change, and extraction time is short, and yield is than advantages of higher.
The specific embodiment
Below by specific embodiment, the present invention is further illustrated, but protection content of the present invention is not limited to following examples.
In the embodiment of the present invention, adopt following methods to carry out the pretreatment of following steps to dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee: above raw material is pulverized, be placed at 30-60 ℃ of temperature dry, its moisture is down to below 5%, then cross the 20-100 mesh sieve, obtain the powder of above raw material, standby.
Embodiment 1 associated extraction liquid preparation
The extract preparation:
(1) in hydrogen peroxide solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH hydrogen peroxide solution or potassium hydroxide hydrogen peroxide solution or calcium hydroxide hydrogen peroxide solution or ammoniacal liquor hydrogen peroxide solution or be added with the hydrogen peroxide solution of phosphate buffer or be added with the hydrogen peroxide solution of borax-calcium chloride buffer solution or be added with the hydrogen peroxide solution of ammonia-ammonium chloride buffer;
(2) in the liquor natrii hypochloritis, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH liquor natrii hypochloritis or potassium hydroxide liquor natrii hypochloritis or calcium hydroxide liquor natrii hypochloritis or ammoniacal liquor liquor natrii hypochloritis or be added with the liquor natrii hypochloritis of phosphate buffer or be added with the liquor natrii hypochloritis of borax-calcium chloride buffer solution or be added with the liquor natrii hypochloritis of ammonia-ammonium chloride buffer;
(3) in postassium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH postassium hypochlorite solution or potassium hydroxide postassium hypochlorite solution or calcium hydroxide postassium hypochlorite solution or ammoniacal liquor postassium hypochlorite solution or be added with the postassium hypochlorite solution of phosphate buffer or be added with the postassium hypochlorite solution of borax-calcium chloride buffer solution or be added with the postassium hypochlorite solution of ammonia-ammonium chloride buffer;
(4) in calcium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH calcium hypochlorite solution or potassium hydroxide calcium hypochlorite solution or calcium hydroxide calcium hypochlorite solution or ammoniacal liquor calcium hypochlorite solution or be added with the calcium hypochlorite solution of phosphate buffer or be added with the calcium hypochlorite solution of borax-calcium chloride buffer solution or be added with the calcium hypochlorite solution of ammonia-ammonium chloride buffer.
Wherein
Phosphate buffered formula of liquid and preparation method are:
(1) get NaH 2PO 42H 2O or NaH 2PO 4H 2O, add redistilled water and dissolve to 1000ml, is mixed with the Na of 0.2mol/L 2HPO 4Solution;
(2) get Na 2HPO 412H 2O or Na 2HPO 47H 2O or Na 2HPO 42H 2O, add redistilled water and dissolve to 1000ml, is mixed with the Na of 0.2mol/L 2HPO 4Solution;
(3) NaH of the 0.2mol/L prepared by step (2) 2PO 4The Na of solution and 0.2mol/L 2HPO 412H 2O solution fully mixes and obtains phosphate buffer, by adjusting NaH 2PO 4Solution and Na 2HPO 412H 2The O liquor capacity, than regulating its pH to 7.5-10.0, saves backup under room temperature.
Described borax calcium chloride buffer formulation and preparation method are: get borax, calcium chloride, after adding water 800ml dissolving, regulate pH to 7.5-10.0 with the 1mol/L hydrochloric acid solution, is diluted with water to 1000ml, obtains borax-calcium chloride buffer solution.
Described-ammonia chloride buffer formula of liquid and preparation method are: get ammonium chloride, be dissolved in water to 100ml, then add liquor ammoniae dilutus adjusting pH to 7.5-10.0, obtain ammonia-ammonium chloride buffer.
Embodiment 2
Get the pretreated rapeseed cake dregs of 1g or rapeseed hull (to not restriction of rape variety, the method of pretreatment of raw material is: apply conventional pulverizer above raw material is pulverized, be placed under 30-60 ℃ dry, its moisture is down to below 5%, then cross the 20-100 mesh sieve, obtain the powder of pretreated raw material) powder, then add the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares in raw material wherein, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), at 50 ℃ of lower lixiviate 3h, remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor, analytical method adopts Folin-phenol colorimetric method (Liu Qing etc., the content [J] of total polyphenols in Folin-Ciocalteu colorimetric method for determining barley plants polyphenol extract. food science and technology, 2007, 32 (4): 175-177) in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.57mg/g.
Embodiment 3
According to 2 methods that provide of enforcement, carry out.Get the pretreated peanut shell powder of 1g, then add wherein the hydrogen peroxide solution extract that is added with borax calcium chloride buffer solution (pH is 7.77) that embodiment 1 prepares, wherein the pH of borax calcium chloride buffer solution is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 55 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.99mg/g.
Embodiment 4
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment apple powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with the ammonia ammonium chloride buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of ammonia ammonium chloride buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 60 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.16mg/g.
Embodiment 5
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment persimmon powder afterwards, then add wherein the NaOH hydrogen peroxide solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 6
According to 2 methods that provide of enforcement, carry out.Get the pretreated grape powder of 1g, then add wherein the potassium hydroxide hydrogen peroxide solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is 1.5mL(H 2O 2With the volume mass of raw material, compare for 1.5:1), then at 70 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 7
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment citrus powder afterwards, then add wherein the calcium hydroxide hydrogen peroxide solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 2h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.13mg/g.
Embodiment 8
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment radish powder afterwards, then add wherein the ammoniacal liquor hydrogen peroxide solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 2.5h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.77mg/g.
Embodiment 9
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment powdered soybean afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.0h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 10
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment jujube powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 10.17mg/g.
Embodiment 11
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment Pomegranate Rind afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 4.0h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 12
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment banana skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 10mL(raw material and plant polyphenol extract is 1:10), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), the compound method of buffer solution used is: get borax 0.572g and calcium chloride 2.94g, after adding the about 800ml dissolving of water, regulating the pH value with the 1mol/L hydrochloric acid solution is 7.7, be diluted with water to 1000ml, obtain pH=7.7 borax-calcium chloride buffer solution.Then at 65 ℃ of lower lixiviate 3.5h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 10.77mg/g.
Embodiment 13
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment sweet potato powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 15mL(raw material and plant polyphenol extract is 1:15), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 11.36mg/g.
Embodiment 14
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment hawthorn skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 11.42mg/g.
Embodiment 15
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment tomato powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its ul-trasonic irradiation in 0W power to process 0min in leaching process and do not carry out the ultrasonic wave processing.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 10.50mg/g.
Embodiment 16
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment pine nut (powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 30mL(raw material and plant polyphenol extract is 1:30), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its ul-trasonic irradiation in 0W power to process 15min in leaching process and do not carry out the ultrasonic wave processing.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 10.08mg/g.
Embodiment 17
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment oat powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 0.6mL(hydrogen peroxide is 0.6:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its ul-trasonic irradiation in 0W power to process 30min in leaching process and do not carry out the ultrasonic wave processing.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 5.44mg/g.
Embodiment 18
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment lichee powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 1.5mL(hydrogen peroxide is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its ul-trasonic irradiation in 5000W power to process 0min in leaching process and do not carry out the ultrasonic wave processing.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Fol in phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 11.42mg/g.
Embodiment 19
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 2.4mL(hydrogen peroxide is 2.4:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 15min under its ul-trasonic irradiation in 5000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 17.01mg/g.
Embodiment 20
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 3.3mL(hydrogen peroxide is 3.3:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 30min under its ul-trasonic irradiation in 5000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.60mg/g.
Embodiment 21
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 4.2mL(hydrogen peroxide is 4.2:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its ul-trasonic irradiation in 10000W power to process 0min in leaching process and do not carry out the ultrasonic wave processing.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.68mg/g.
Embodiment 22
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), the compound method of buffer solution used is: get ammonium chloride 1.07g, add water and make to be dissolved into 100ml, adding liquor ammoniae dilutus adjusting pH value is 7.7 again, obtain ammonia-ammonium chloride buffer of pH=7.7.Then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 15min under its ul-trasonic irradiation in 10000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 20.95mg/g.
Embodiment 23
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 6mL(hydrogen peroxide is 6:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 0min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 20.19mg/g.
Embodiment 24
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 6.9mL(hydrogen peroxide is 6.9:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 0.5min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.84mg/g.
Embodiment 25
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 26
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with borax-calcium chloride buffer solution (pH is 7.5) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 27
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with the ammonia ammonium chloride buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 28
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the NaOH hydrogen peroxide solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 29
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the potassium hydroxide hydrogen peroxide solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 30
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hydroxide hydrogen peroxide solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 31
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the ammoniacal liquor hydrogen peroxide solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 32
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 8.0) that embodiment 1 prepares, wherein the pH of phosphate buffer is 8.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 40000W power to process 0min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.05mg/g.
Embodiment 33
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 8.5) that embodiment 1 prepares, wherein the pH of phosphate buffer is 8.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 40000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.53mg/g.
Embodiment 34
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 9.0) that embodiment 1 prepares, wherein the pH of phosphate buffer is 9.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 1min under its microwave action in 40000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 20.64mg/g.
Embodiment 35
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 9.5) that embodiment 1 prepares, wherein the pH of phosphate buffer is 9.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 80000W power to process 0min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.97mg/g.
Embodiment 36
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of phosphate buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 37
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with borax calcium chloride buffer solution (pH is 10.0) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 38
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with ammonia-ammonium chloride buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 39
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the NaOH hydrogen peroxide solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 40
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the potassium hydroxide hydrogen peroxide solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 41
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hydroxide hydrogen peroxide solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 42
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the ammoniacal liquor hydrogen peroxide solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 43
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 30min, in leaching process, make to process 30min under its ul-trasonic irradiation in 10000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 28.96mg/g.
Embodiment 44
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 1min, in leaching process, make to process 1min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 30.34mg/g.
Embodiment 45
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 46
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of phosphate buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 47
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 48
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the hydrogen peroxide solution extract that is added with phosphate buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of phosphate buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of hydrogen peroxide is that the 5.1mL(hydrogen peroxide is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 49
Get the pretreated rapeseed cake dregs of 1g or rapeseed hull (to not restriction of rape variety, the method of pretreatment of raw material is: apply conventional pulverizer above raw material is pulverized, be placed under 30-60 ℃ dry, its moisture is down to below 5%, then cross the 20-100 mesh sieve, obtain the powder of pretreated raw material) powder, then add the liquor natrii hypochloritis's extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares in raw material wherein, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is that the 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), at 50 ℃ of lower lixiviate 3h, remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor, analytical method adopts Folin-phenol colorimetric method (Liu Qing etc., the content [J] of total polyphenols in Folin-Ciocalteu colorimetric method for determining barley plants polyphenol extract. food science and technology, 2007, 32 (4): 175177) in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.57mg/g.
Embodiment 50
According to 2 methods that provide of enforcement, carry out.Get the pretreated peanut shell powder of 1g, then add wherein the liquor natrii hypochloritis's extract that is added with borax-calcium chloride buffer solution (pH is 7.77) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is that the 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then at 55 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.99mg/g.
Embodiment 51
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment apple powder afterwards, then add wherein the liquor natrii hypochloritis's extract that is added with the ammonia ammonium chloride buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of ammonia ammonium chloride buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is that the 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then at 60 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.16mg/g.
Embodiment 52
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment persimmon powder afterwards, then add wherein the NaOH liquor natrii hypochloritis extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is that the 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 53
According to 2 methods that provide of enforcement, carry out.Get the pretreated grape powder of 1g, then add wherein the potassium hydroxide liquor natrii hypochloritis extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is that the 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then at 70 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 54
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment citrus powder afterwards, then add wherein the calcium hydroxide liquor natrii hypochloritis extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is that the 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 2h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.13mg/g.
Embodiment 55
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment radish powder afterwards, then add wherein the ammoniacal liquor liquor natrii hypochloritis extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of clorox is that the 1.5mL(clorox is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 2.5h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.77mg/g.
Embodiment 56
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the liquor natrii hypochloritis's extract that is added with phosphate buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 57
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the liquor natrii hypochloritis's extract that is added with borax-calcium chloride buffer solution (pH is 7.5) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 58
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the liquor natrii hypochloritis's extract that is added with ammonia-ammonium chloride buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 59
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the NaOH liquor natrii hypochloritis extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 60
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the potassium hydroxide liquor natrii hypochloritis extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 61
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hydroxide liquor natrii hypochloritis extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 62
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the ammoniacal liquor liquor natrii hypochloritis extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 63
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the liquor natrii hypochloritis's extract that is added with phosphate buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of phosphate buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 64
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the liquor natrii hypochloritis's extract that is added with borax-calcium chloride buffer solution (pH is 10.0) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 65
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the liquor natrii hypochloritis's extract that is added with ammonia-ammonium chloride buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 66
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the NaOH liquor natrii hypochloritis extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 67
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the potassium hydroxide liquor natrii hypochloritis extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 68
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hydroxide liquor natrii hypochloritis extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 69
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the ammoniacal liquor liquor natrii hypochloritis extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of clorox is that the 5.1mL(clorox is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 70
Get the pretreated rapeseed cake dregs of 1g or rapeseed hull (to not restriction of rape variety, the method of pretreatment of raw material is: apply conventional pulverizer above raw material is pulverized, be placed under 30-60 ℃ dry, its moisture is down to below 5%, then cross the 20-100 mesh sieve, obtain the powder of pretreated raw material) powder, then add the postassium hypochlorite solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares in raw material wherein, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is that the 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), at 50 ℃ of lower lixiviate 3h, remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor, analytical method adopts Folin-phenol colorimetric method (Liu Qing etc., the content [J] of total polyphenols in Folin-Ciocalteu colorimetric method for determining barley plants polyphenol extract. food science and technology, 2007, 32 (4): 175-177) in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.57mg/g.
Embodiment 71
According to 2 methods that provide of enforcement, carry out.Get the pretreated peanut shell powder of 1g, then add wherein the postassium hypochlorite solution extract that is added with borax-calcium chloride buffer solution (pH is 7.77) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is that the 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 55 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.99mg/g.
Embodiment 72
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment apple powder afterwards, then add wherein the postassium hypochlorite solution extract that is added with ammonia-ammonium chloride buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is that the 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 60 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.16mg/g.
Embodiment 73
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment persimmon powder afterwards, then add wherein the NaOH postassium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is that the 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 74
According to 2 methods that provide of enforcement, carry out.Get the pretreated grape powder of 1g, then add wherein the potassium hydroxide postassium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is that the 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 70 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 75
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment citrus powder afterwards, then add wherein the calcium hydroxide postassium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is that the 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 2h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.13mg/g.
Embodiment 76
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment radish powder afterwards, then add wherein the ammoniacal liquor postassium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of postassium hypochlorite is that the 1.5mL(postassium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 2.5h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.77mg/g.
Embodiment 77
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the postassium hypochlorite solution extract that is added with phosphate buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 78
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the postassium hypochlorite solution extract that is added with borax calcium chloride buffer solution (pH is 7.5) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 79
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the postassium hypochlorite solution extract that is added with ammonia-ammonium chloride buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 80
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the NaOH postassium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 81
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the potassium hydroxide postassium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 82
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hydroxide postassium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 83
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the ammoniacal liquor postassium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 84
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the postassium hypochlorite solution extract that is added with phosphate buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of phosphate buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 85
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the postassium hypochlorite solution extract that is added with borax calcium chloride buffer solution (pH is 10.0) that embodiment 1 prepares, wherein the pH of borax calcium chloride buffer solution is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 86
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the postassium hypochlorite solution extract that is added with the ammonia ammonium chloride buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 87
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the NaOH postassium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 88
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the potassium hydroxide postassium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 89
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hydroxide postassium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 90
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the ammoniacal liquor postassium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of postassium hypochlorite is that the 5.1mL(postassium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 91
Get the pretreated rapeseed cake dregs of 1g or rapeseed hull (to not restriction of rape variety, the method of pretreatment of raw material is: apply conventional pulverizer above raw material is pulverized, be placed under 30-60 ℃ dry, its moisture is down to below 5%, then cross the 20-100 mesh sieve, obtain the powder of pretreated raw material) powder, then add the calcium hypochlorite solution extract that is added with phosphate buffer (pH is 7.77) that embodiment 1 prepares in raw material wherein, wherein the pH of phosphate buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is that the 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), at 50 ℃ of lower lixiviate 3h, remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor, analytical method adopts Folin-phenol colorimetric method (Liu Qing etc., the content [J] of total polyphenols in Folin-Ciocalteu colorimetric method for determining barley plants polyphenol extract. food science and technology, 2007, 32 (4): 175-177) in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.57mg/g.
Embodiment 92
According to 2 methods that provide of enforcement, carry out.Get the pretreated peanut shell powder of 1g, then add wherein the calcium hypochlorite solution extract that is added with borax-calcium chloride buffer solution (pH is 7.77) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is that the 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 55 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 8.99mg/g.
Embodiment 93
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment apple powder afterwards, then add wherein the calcium hypochlorite solution extract that is added with ammonia-ammonium chloride buffer (pH is 7.77) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 7.7, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is that the 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 60 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.16mg/g.
Embodiment 94
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment persimmon powder afterwards, then add wherein the NaOH calcium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is that the 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.94mg/g.
Embodiment 95
According to 2 methods that provide of enforcement, carry out.Get the pretreated grape powder of 1g, then add wherein the potassium hydroxide calcium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is that the 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 70 ℃ of lower lixiviate 3h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.85mg/g.
Embodiment 96
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment citrus powder afterwards, then add wherein the calcium hydroxide calcium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is that the 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 2h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.13mg/g.
Embodiment 97
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment radish powder afterwards, then add wherein the ammoniacal liquor calcium hypochlorite solution extract (pH is 7.77) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 25mL(raw material and plant polyphenol extract is 1:25), wherein the volume of calcium hypochlorite is that the 1.5mL(calcium hypochlorite is 1.5:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 2.5h.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 9.77mg/g.
Embodiment 98
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hypochlorite solution extract that is added with phosphate buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of phosphate buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 99
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hypochlorite solution extract that is added with borax-calcium chloride buffer solution (pH is 7.5) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 100
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hypochlorite solution extract that is added with ammonia-ammonium chloride buffer (pH is 7.5) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 7.5, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 101
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the NaOH calcium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 102
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the potassium hydroxide calcium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 103
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hydroxide calcium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 104
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the ammoniacal liquor calcium hypochlorite solution extract (pH is 7.5) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, make under its microwave action in 0W power to process 1min in leaching process and do not carry out Microwave Treatment.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 18.18mg/g.
Embodiment 105
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hypochlorite solution extract that is added with phosphate buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of phosphate buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 106
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hypochlorite solution extract that is added with borax-calcium chloride buffer solution (pH is 10.0) that embodiment 1 prepares, wherein the pH of borax-calcium chloride buffer solution is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 107
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hypochlorite solution extract that is added with ammonia-ammonium chloride buffer (pH is 10.0) that embodiment 1 prepares, wherein the pH of ammonia-ammonium chloride buffer is 10.0, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 108
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the NaOH calcium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 109
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the potassium hydroxide calcium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 110
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the calcium hydroxide calcium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.
Embodiment 111
According to 2 methods that provide of enforcement, carry out.Get 1g pretreatment dregs of rapeseed cake or vegetable seed skin powder afterwards, then add wherein the ammoniacal liquor calcium hypochlorite solution extract (pH is 10.0) that embodiment 1 prepares, the mass volume ratio that the volume of plant polyphenol extract used is 20mL(raw material and plant polyphenol extract is 1:20), wherein the volume of calcium hypochlorite is that the 5.1mL(calcium hypochlorite is 5.1:1 with the volume mass ratio of raw material), then at 65 ℃ of lower lixiviate 3.5h, in leaching process, make to process 0.5min under its microwave action in 80000W power.Remove residue (remaining raw material after being lixiviate) through centrifugation, obtain containing the plant polyphenol leaching liquor.Adopt Folin-phenol colorimetric method in the 765nm colorimetric estimation, the plant polyphenol yield of the present embodiment is 19.73mg/g.

Claims (5)

  1. A skin from rapeseed meal or rapeseed, peanut shells, apples, persimmons, grapes, oranges, carrots, beans, dates, peel, banana peel, sweet potato, hawthorn skin, tomatoes, nuts, oats, lychee plant polyphenols extracted extract, characterized in that: the sodium hydroxide extract to pH 7.5 to 5.0 solution of hydrogen peroxide, sodium hypochlorite or sodium hydroxide solution to pH 7.5 to 10.0, or pH 7.5 to 5.0 sodium hydroxide solution, potassium hypochlorite, calcium hypochlorite or sodium hydroxide solution to pH 7.5 to 5.0 or pH 7.5 for the potassium hydroxide solution to 10.0 and hydrogen peroxide, sodium hypochlorite or potassium hydroxide solution, pH 7.5 to 10.0, potassium hydroxide or potassium hypochlorite at pH 7.5 to 5.0 solution of calcium hypochlorite or potassium hydroxide solution, pH 7.5 to 10.0, or pH of a solution of hydrogen peroxide, calcium hydroxide, 7.5 to 10.0, or pH 7.5 to 10.0 sodium hypochlorite, calcium hydroxide, or calcium hydroxide to pH 7.5 to 5.0 potassium hypochlorite solution, the pH of calcium hydroxide or calcium hypochlorite solution, 7.5 to 10.0,Solution of hydrogen peroxide or ammonia to pH 7.5 to 5.0, the sodium hypochlorite solution or aqueous ammonia to pH 7.5 to 5.0, the pH of aqueous ammonia or potassium hypochlorite solution of 7.5 to 5.0, ammonia or calcium hypochlorite solution to pH 7.5 to 10.0, or pH 7.5 to 5.0 of hydrogen peroxide solution was added with phosphate buffer, pH 7.5 to 5.0 or a sodium hypochlorite solution was added with phosphate buffer, pH 7.5 to 5.0, or added with potassium hypochlorite solution of phosphate buffer, or pH 7.5 to 5.0 with the addition of calcium hypochlorite solution is a phosphate buffer, pH 7.5 to 5.0, or plus borax - hydrogen peroxide solution of calcium chloride buffer pH 7.5 to 5.0, or plus borax - sodium hypochlorite, calcium chloride buffer solution, pH 7.5 to 5.0, or plus borax - potassium hypochlorite solution of calcium chloride buffer, pH 7.5 to 5.0, or plus borax - hypochlorous acid chloride buffer calcium solution or pH 7.5 to 5.0 plus ammonia - hydrogen peroxide solution of ammonium chloride buffer, pH 7.5 to 5.0, or plus ammonia - ammonium chloride buffer solution of sodium hypochlorite or pH 7.5 to 5.0 with the addition ammonia - ammonium chloride buffer, potassium hypochlorite solution, pH 7.5 to 5.0, or plus ammonia - ammonium chloride buffer solution of calcium hypochlorite;
    Described extract recipe and preparation method are:
    (1) in hydrogen peroxide solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH hydrogen peroxide solution or potassium hydroxide hydrogen peroxide solution or calcium hydroxide hydrogen peroxide solution or ammoniacal liquor hydrogen peroxide solution or be added with the hydrogen peroxide solution of phosphate buffer or be added with the hydrogen peroxide solution of borax-calcium chloride buffer solution or be added with the hydrogen peroxide solution of ammonia-ammonium chloride buffer;
    (2) in the liquor natrii hypochloritis, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH liquor natrii hypochloritis or potassium hydroxide liquor natrii hypochloritis or calcium hydroxide liquor natrii hypochloritis or ammoniacal liquor liquor natrii hypochloritis or be added with the liquor natrii hypochloritis of phosphate buffer or be added with the liquor natrii hypochloritis of borax-calcium chloride buffer solution or be added with the liquor natrii hypochloritis of ammonia-ammonium chloride buffer;
    (3) in postassium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH postassium hypochlorite solution or potassium hydroxide postassium hypochlorite solution or calcium hydroxide postassium hypochlorite solution or ammoniacal liquor postassium hypochlorite solution or be added with the postassium hypochlorite solution of phosphate buffer or be added with the postassium hypochlorite solution of borax-calcium chloride buffer solution or be added with the postassium hypochlorite solution of ammonia-ammonium chloride buffer;
    (4) in calcium hypochlorite solution, add NaOH or potassium hydroxide or calcium hydroxide or ammoniacal liquor or phosphate buffer or borax-calcium chloride buffer solution or ammonia-ammonium chloride buffer to obtain NaOH calcium hypochlorite solution or potassium hydroxide calcium hypochlorite solution or calcium hydroxide calcium hypochlorite solution or ammoniacal liquor calcium hypochlorite solution or be added with the calcium hypochlorite solution of phosphate buffer or be added with the calcium hypochlorite solution of borax-calcium chloride buffer solution or be added with the calcium hypochlorite solution of ammonia-ammonium chloride buffer;
    Described phosphate buffered formula of liquid and preparation method are:
    (1) get NaH 2PO 42H 2O or NaH 2PO 4H 2O, add redistilled water and dissolve to 1000ml, is mixed with the Na of solution 0.2mol/L 2HPO 4Solution;
    (2) get Na 2HPO 412H 2O or Na 2HPO 47H 2O or Na 2HPO 42H 2O, add redistilled water and dissolve to 1000ml, is mixed with the Na of 0.2mol/L 2HPO 4Solution;
    (3) NaH of the 0.2mol/L prepared by step (2) 2PO 4The Na of solution and 0.2mol/L 2HPO 412H 2O solution fully mixes and obtains phosphate buffer, by adjusting NaH 2PO 4Solution and Na 2HPO 412H 2The O liquor capacity is than regulating its pH to 7.5 ~ 10.0; Described borax-calcium chloride buffer formulation and preparation method are: get borax, calcium chloride, after adding water 800ml dissolving, regulate pH to 7.5 ~ 10.0 with the 1mol/L hydrochloric acid solution, is diluted with water to 1000ml, obtains borax-calcium chloride buffer solution;
    Described ammonia-chloride buffer formula of liquid and preparation method are: get ammonium chloride, be dissolved in water to 100ml, then add liquor ammoniae dilutus adjusting pH to 7.5 ~ 10.0, obtain ammonia-ammonium chloride buffer.
  2. 2. one kind is utilized extract claimed in claim 1 from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, the hawthorn skin, tomato, nut, extract the extracting method of plant polyphenol in oat or lichee, it is characterized in that: above-described raw material is pulverized, add extract claimed in claim 1 in one of raw material wherein, the mass volume ratio of described raw material and extract is 1:15 ~ 1:30, under 50 ~ 70 ℃, stir, extract 2.5 ~ 4.0 hours, remove residue through centrifugation, obtain the leaching liquor that contains plant polyphenol.
  3. 3. the extracting method of extracting plant polyphenol from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee according to claim 2, it is characterized in that: above-described raw material is pulverized, be placed under 30 ~ 60 ℃ dry, make below its moisture to 5%, then cross 20 ~ 100 mesh sieves, obtain the powder of above raw material.
  4. 4. the extracting method of extracting plant polyphenol from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee according to claim 2 is characterized in that: the volume mass of described hydrogen peroxide or clorox or postassium hypochlorite or calcium hypochlorite and described raw material is than being 2.4:1 ~ 6:1.
  5. 5. the extracting method of extracting plant polyphenol from dregs of rapeseed cake or vegetable seed skin, peanut shell, apple, persimmon, grape, citrus, radish, soybean, jujube, granatum, banana skin, sweet potato, hawthorn skin, tomato, nut, oat, lichee according to claim 2, it is characterized in that: add in above raw material in the process of lixiviate after described extract, make under its ultrasonic wave that is 0 ~ 10000W in power to process the lower processing of microwave 0 ~ 1min that 0 ~ 30min or power are 0 ~ 80000W.
CN201210154936.0A 2012-05-18 2012-05-18 A kind of extract and extracting method extracting plant polyphenol Expired - Fee Related CN103418160B (en)

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CN105129948A (en) * 2015-09-06 2015-12-09 河南城建学院 Method for extracting natural flocculation active ingredients from pomegranate bark and method for preparing flocculating agent
CN107929151A (en) * 2017-12-24 2018-04-20 姚佑灿 A kind of skin moisture-keeping remediation composition
CN108516995A (en) * 2018-04-27 2018-09-11 湖南口味王集团有限责任公司 A kind of method of purification of betel nut polyphenol

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CN1121926A (en) * 1994-10-22 1996-05-08 王崴 Extraction method and application of plant protein
CN1431231A (en) * 2003-01-29 2003-07-23 广西壮族自治区中国科学院广西植物研究所 Method for extracting xylan from woody fiber material
CN1823856A (en) * 2005-12-24 2006-08-30 安徽乳泉石榴酒业有限公司 Method of extracting polyphenol from pomegranate seed

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CN1121926A (en) * 1994-10-22 1996-05-08 王崴 Extraction method and application of plant protein
CN1431231A (en) * 2003-01-29 2003-07-23 广西壮族自治区中国科学院广西植物研究所 Method for extracting xylan from woody fiber material
CN1823856A (en) * 2005-12-24 2006-08-30 安徽乳泉石榴酒业有限公司 Method of extracting polyphenol from pomegranate seed

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105129948A (en) * 2015-09-06 2015-12-09 河南城建学院 Method for extracting natural flocculation active ingredients from pomegranate bark and method for preparing flocculating agent
CN105129948B (en) * 2015-09-06 2017-11-14 河南城建学院 A kind of Small-scale Landscape Water Bodies being used for using blue-green algae as sociales remove the flocculant preparation method of algae
CN107929151A (en) * 2017-12-24 2018-04-20 姚佑灿 A kind of skin moisture-keeping remediation composition
CN108516995A (en) * 2018-04-27 2018-09-11 湖南口味王集团有限责任公司 A kind of method of purification of betel nut polyphenol

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