CN105399706A - Method for extracting vitamins from grapes - Google Patents
Method for extracting vitamins from grapes Download PDFInfo
- Publication number
- CN105399706A CN105399706A CN201510890600.4A CN201510890600A CN105399706A CN 105399706 A CN105399706 A CN 105399706A CN 201510890600 A CN201510890600 A CN 201510890600A CN 105399706 A CN105399706 A CN 105399706A
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- CN
- China
- Prior art keywords
- grape
- vitamin
- method extracting
- vacuum
- grapes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 235000013343 vitamin Nutrition 0.000 title claims abstract description 38
- 239000011782 vitamin Substances 0.000 title claims abstract description 38
- 229940088594 vitamin Drugs 0.000 title claims abstract description 38
- 229930003231 vitamin Natural products 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000219094 Vitaceae Species 0.000 title abstract 5
- 235000021021 grapes Nutrition 0.000 title abstract 5
- 241000219095 Vitis Species 0.000 claims abstract description 46
- 235000009754 Vitis X bourquina Nutrition 0.000 claims abstract description 46
- 235000012333 Vitis X labruscana Nutrition 0.000 claims abstract description 46
- 235000014787 Vitis vinifera Nutrition 0.000 claims abstract description 46
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 34
- 238000003756 stirring Methods 0.000 claims abstract description 17
- 239000000706 filtrate Substances 0.000 claims description 17
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- 238000003828 vacuum filtration Methods 0.000 claims description 14
- 239000000284 extract Substances 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 12
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 10
- 239000000047 product Substances 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 8
- 239000002002 slurry Substances 0.000 claims description 8
- 239000001307 helium Substances 0.000 claims description 6
- 229910052734 helium Inorganic materials 0.000 claims description 6
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims description 6
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 5
- 239000007789 gas Substances 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 6
- 239000012074 organic phase Substances 0.000 abstract description 4
- 239000002904 solvent Substances 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 238000001035 drying Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 238000004537 pulping Methods 0.000 abstract 3
- 239000012071 phase Substances 0.000 abstract 2
- 230000001681 protective effect Effects 0.000 abstract 2
- 239000012043 crude product Substances 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 9
- 238000010009 beating Methods 0.000 description 4
- 241000208340 Araliaceae Species 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 3
- 235000003140 Panax quinquefolius Nutrition 0.000 description 3
- 235000008434 ginseng Nutrition 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000013517 stratification Methods 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 241000628997 Flos Species 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 244000194101 Ginkgo biloba Species 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- UEZVMMHDMIWARA-UHFFFAOYSA-N Metaphosphoric acid Chemical compound OP(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
- C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D307/62—Three oxygen atoms, e.g. ascorbic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a method for extracting vitamins from grapes. The method includes the following steps that firstly, grapes are selected and washed clean, the grapes are slit in an extracting protective solvent, and seeds are removed; secondly, shredded grape pulp and the extraction protective solvent are added into a vacuum pulping machine together, and pulping is performed; thirdly, paste generated after pulping is added into a vacuum stirrer and stirred in vacuum; fourthly, materials generated in the third step are filtered, filter liquor stands still and is layered, and an organic phase is separated out; fifthly, water with pH smaller than 7 is added into the organic phase obtained in the fourth step, and vacuum stirring is performed for 2 or more hours; sixthly, standing and layering are performed, and a water phase is separated out; seventhly, the water phase is concentrated to be dry in vacuum, and vitamin crude products are obtained after freezing and drying. Due to the method, the vitamins in the grapes can be extracted; the extraction method is simple and suitable for being applied and popularized on a large scale; the content of the vitamins in extractives is high.
Description
Technical field
The present invention relates to deep-processing technical field of agricultural products, particularly a kind of method extracting VITAMIN from grape.
Background technology
VITAMIN is humans and animals is the class trace organic substance maintaining normal physiological function and must obtain from food, plays an important role in growth in humans, metabolism, growth course.
VITAMIN as a class biologically active substance, easy inactivation under the condition such as high temperature, strong acid and strong base.Therefore the extraction of VITAMIN needs the difficult problem overcoming its volatility.Chinese patent CN201510097751(application number) disclose and a kind of from Fructus Chaenomelis Chinesis, extract ascorbic method, comprise the steps: that (1) wins Fructus Chaenomelis Chinesis fresh fruit, clean, cut in extraction protection solvent, remove seed; (2) the Fructus Chaenomelis Chinesis pulp after chopping is joined in vacuum beating machine together with extraction protection solvent, making beating; (3) slurry produced after making beating is joined in vacuum mixer, vacuum stirring; (4) material filtering will produced in step (3), by filtrate stratification, isolates organic phase; (5) water that pH is less than 7 is added in the organic phase obtained in step (4), vacuum stirring more than 2 hours; (6) stratification, isolates aqueous phase; (7) aqueous phase vacuum concentration is extremely dry, and obtain the thick product of vitamins C after lyophilize.Chinese patent CN201410813961(application number) disclose the ascorbic extracting method of a kind of Herba Herminii, (1) cleaning of fresh ginseng fruit, cold sterilization, then add Folium Ginkgo extract, Flos Lonicerae extract and S-WAT, making beating, obtains ginseng pulp; (2) according to weight percent meter, take the aqueous solution of 2-3% oxalic acid, 0.5-1% hydrochloric acid and 0.5-1% metaphosphoric acid as extracting solution, according to extracting solution: the volume ratio of ginseng pulp is 5-8:1, at 40-60 DEG C, soak 30-1min, the filtrate of filtration, extracts 2-3 time; (3) merging filtrate, then concentrate drying, obtain Herba Herminii vitamins C.But it is long and extract insufficient problem to there is extracting cycle in such scheme.
Vitamin is worth very high, and wide market, develops a kind of method rapidly and efficiently extracting VITAMIN, very important to the exploitation of VITAMIN.
Summary of the invention
For the deficiencies in the prior art, the object of the present invention is to provide a kind of method extracting VITAMIN from grape.
Technical scheme of the present invention is achieved in that
From grape, extract a method for VITAMIN, comprise the steps:
(1) choose new fresh grape, clean, quick stripping and slicing, puts into stirrer;
(2) in stirrer, add 0.5-5% acid solution and granulated glass sphere, pass into protection gas agitating 5-30 minute;
(3) slurries produced after stirring are carried out vacuum filtration;
(4) add gac stirring in the filtrate produced in step (3) to decolour for 1-5 minute, vacuum filtration again;
(5) filtrate obtained in step (4) is carried out vacuum lyophilization, obtain the thick product of VITAMIN.
Preferably, the weight ratio of described acid solution and grape is 10-50:1.
Preferably, described acid solution is the combination of any one or they in acetic acid, phosphoric acid.
Preferably, its diameter of described granulated glass sphere is 0.5-1mm.
Preferably, the weight ratio of described granulated glass sphere and grape is 1:100-500.
Preferably, described protection gas is nitrogen or helium.
Preferably, described in step (2), mixing control temperature is 10-16 DEG C.
Preferably, the weight ratio of described gac and grape is 1:200-1000.
Preferably, its temperature of described vacuum lyophilization is-15 DEG C to-25 DEG C.
The invention has the beneficial effects as follows:
1. acidic solution extracts the activity that can keep VITAMIN.
2. granulated glass sphere can quick crashing cell, improves extraction efficiency.
3. protect gas that VITAMIN can be avoided oxidized.
4. charcoal absorption can remove impurity.
5. the present invention is simple to operate, with low cost, can produce high-quality vitamin products.
Embodiment
embodiment 1
From grape, extract a method for VITAMIN, comprise the steps:
(1) choose new fresh grape, clean, quick stripping and slicing, puts into stirrer;
(2) adding in stirrer with the weight ratio of grape is the 1.5% acetic acid acid solution of 35:1, and the diameter being 1:300 with the weight ratio of grape is the granulated glass sphere of 0.5mm, passes into nitrogen, and 16 DEG C are stirred 15 minutes;
(3) slurries produced after stirring are carried out vacuum filtration;
(4) adding with the weight ratio of grape in the filtrate produced in step (3) is that the gac of 1:600 stirs and decolours for 2 minutes, vacuum filtration again;
(5) filtrate obtained in step (4) is carried out vacuum lyophilization at-20 DEG C, obtain the thick product of VITAMIN.
embodiment 2
From grape, extract a method for VITAMIN, comprise the steps:
(1) choose new fresh grape, clean, quick stripping and slicing, puts into stirrer;
(2) adding in stirrer with the weight ratio of grape is 0.5% phosphoric acid solution of 10:1, and the diameter being 1:100 with the weight ratio of grape is the granulated glass sphere of 1mm, passes into helium, and 10 DEG C are stirred 30 minutes;
(3) slurries produced after stirring are carried out vacuum filtration;
(4) adding with the weight ratio of grape in the filtrate produced in step (3) is that the gac of 1:200 stirs and decolours for 5 minutes, vacuum filtration again;
(5) filtrate obtained in step (4) is carried out vacuum lyophilization at-15 DEG C, obtain the thick product of VITAMIN.
embodiment 3
From grape, extract a method for VITAMIN, comprise the steps:
(1) choose new fresh grape, clean, quick stripping and slicing, puts into stirrer;
(2) adding in stirrer with the weight ratio of grape is 5% acetic acid, the phosphoric acid solution of 50:1, and the diameter being 1:500 with the weight ratio of grape is the granulated glass sphere of 1mm, passes into helium, and 12 DEG C are stirred 5 minutes;
(3) slurries produced after stirring are carried out vacuum filtration;
(4) adding with the weight ratio of grape in the filtrate produced in step (3) is that the gac of 1:1000 stirs and decolours for 5 minutes, vacuum filtration again;
(5) filtrate obtained in step (4) is carried out vacuum lyophilization at-25 DEG C, obtain the thick product of VITAMIN.
embodiment 4
From grape, extract a method for VITAMIN, comprise the steps:
(1) choose new fresh grape, clean, quick stripping and slicing, puts into stirrer;
(2) adding in stirrer with the weight ratio of grape is 4% acetum of 150:1, and the diameter being 1:150 with the weight ratio of grape is the granulated glass sphere of 0.5mm, passes into helium, and 15 DEG C are stirred 25 minutes;
(3) slurries produced after stirring are carried out vacuum filtration;
(4) adding with the weight ratio of grape in the filtrate produced in step (3) is that the gac of 1:400 stirs and decolours for 3 minutes, vacuum filtration again;
(5) filtrate obtained in step (4) is carried out vacuum lyophilization at-25 DEG C, obtain the thick product of VITAMIN.
embodiment 5
(1) choose new fresh grape, clean, quick stripping and slicing, puts into stirrer;
(2) adding in stirrer with the weight ratio of grape is 0.5% acetic acid, the phosphoric acid solution of 45:1, and the diameter being 1:250 with the weight ratio of grape is the granulated glass sphere of 0.5mm, passes into nitrogen or helium, and 16 DEG C are stirred 20 minutes;
(3) slurries produced after stirring are carried out vacuum filtration;
(4) adding with the weight ratio of grape in the filtrate produced in step (3) is that the gac of 1:300 stirs and decolours for 4 minutes, vacuum filtration again;
(5) filtrate obtained in step (4) is carried out vacuum lyophilization at-18 DEG C, obtain the thick product of VITAMIN.
Above-described embodiment is only for the invention example is clearly described, and the restriction not to the invention embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.All within the spirit and principles in the present invention any apparent change of extending out or variation be still among the protection domain of the invention claim.
Claims (9)
1. from grape, extract a method for VITAMIN, it is characterized in that, comprise the steps:
(1) choose new fresh grape, clean, quick stripping and slicing, puts into stirrer;
(2) in stirrer, add 0.5-5% acid solution and granulated glass sphere, pass into protection gas agitating 5-30 minute;
(3) slurries produced after stirring are carried out vacuum filtration;
(4) add gac stirring in the filtrate produced in step (3) to decolour for 1-5 minute, vacuum filtration again;
(5) filtrate obtained in step (4) is carried out vacuum lyophilization, obtain the thick product of VITAMIN.
2. according to the method extracting VITAMIN from grape described in claim 1, it is characterized in that, the weight ratio of described acid solution and grape is 10-50:1.
3. according to the method extracting VITAMIN from grape described in claim 2, it is characterized in that, described acid solution is the combination of any one or they in acetic acid, phosphoric acid.
4. according to the method extracting VITAMIN from grape described in claim 1, it is characterized in that, its diameter of described granulated glass sphere is 0.5-1mm.
5. according to the method extracting VITAMIN from grape described in claim 4, it is characterized in that, the weight ratio of described granulated glass sphere and grape is 1:100-500.
6. the method extracting VITAMIN from grape described in arbitrary according to claim 1, it is characterized in that, described protection gas is nitrogen or helium.
7. according to the method extracting VITAMIN from grape described in claim 1, it is characterized in that, described in step (2), mixing control temperature is 10-16 DEG C.
8. according to the method extracting VITAMIN from grape described in claim 1, it is characterized in that, the weight ratio of described gac and grape is 1:200-1000.
9. the method extracting VITAMIN from grape described in arbitrary according to claim 1-8, it is characterized in that, its temperature of described vacuum lyophilization is-15 DEG C to-25 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510890600.4A CN105399706A (en) | 2015-12-08 | 2015-12-08 | Method for extracting vitamins from grapes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510890600.4A CN105399706A (en) | 2015-12-08 | 2015-12-08 | Method for extracting vitamins from grapes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105399706A true CN105399706A (en) | 2016-03-16 |
Family
ID=55465508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510890600.4A Pending CN105399706A (en) | 2015-12-08 | 2015-12-08 | Method for extracting vitamins from grapes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105399706A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107319568A (en) * | 2017-06-26 | 2017-11-07 | 浙江海洋大学 | Extract the process equipment and extracting method of grape vitamin |
| CN109380724A (en) * | 2018-12-10 | 2019-02-26 | 凃玉英 | A kind of alcohol-free red wine capsule and preparation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4251449A (en) * | 1975-07-11 | 1981-02-17 | Clarence Schreur | Method of making a solid crystalline composition consisting essentially of calcium ascorbate |
| CN103694205A (en) * | 2013-12-16 | 2014-04-02 | 小金县四姑娘山天然沙棘食品有限公司 | Method for extracting Vc from sea buckthorn |
| CN104610203A (en) * | 2015-03-05 | 2015-05-13 | 国家林业局泡桐研究开发中心 | Method for extracting vitamin C from shine skin papayas |
-
2015
- 2015-12-08 CN CN201510890600.4A patent/CN105399706A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4251449A (en) * | 1975-07-11 | 1981-02-17 | Clarence Schreur | Method of making a solid crystalline composition consisting essentially of calcium ascorbate |
| CN103694205A (en) * | 2013-12-16 | 2014-04-02 | 小金县四姑娘山天然沙棘食品有限公司 | Method for extracting Vc from sea buckthorn |
| CN104610203A (en) * | 2015-03-05 | 2015-05-13 | 国家林业局泡桐研究开发中心 | Method for extracting vitamin C from shine skin papayas |
Non-Patent Citations (4)
| Title |
|---|
| 叶在荣等: "测定维生素C 的提取剂的研究", 《食品工业科技》 * |
| 张燕红等: "《生物化学实验》", 30 September 2013, 华南理工大学出版社 * |
| 童莉等: "葡萄贮藏过程中含糖量、维生素C、呼吸、膜透性的变化和耐贮性的关系", 《种子》 * |
| 罗九甫等: "《生物工程原理与技术》", 30 November 2006, 科学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107319568A (en) * | 2017-06-26 | 2017-11-07 | 浙江海洋大学 | Extract the process equipment and extracting method of grape vitamin |
| CN107319568B (en) * | 2017-06-26 | 2024-01-02 | 浙江海洋大学 | Processing equipment and method for extracting grape vitamins |
| CN109380724A (en) * | 2018-12-10 | 2019-02-26 | 凃玉英 | A kind of alcohol-free red wine capsule and preparation method |
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Application publication date: 20160316 |
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