CN1121926A - Extraction method and application of plant protein - Google Patents
Extraction method and application of plant protein Download PDFInfo
- Publication number
- CN1121926A CN1121926A CN 94116992 CN94116992A CN1121926A CN 1121926 A CN1121926 A CN 1121926A CN 94116992 CN94116992 CN 94116992 CN 94116992 A CN94116992 A CN 94116992A CN 1121926 A CN1121926 A CN 1121926A
- Authority
- CN
- China
- Prior art keywords
- protein
- plant protein
- value
- acid
- losantin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010064851 Plant Proteins Proteins 0.000 title claims abstract description 23
- 235000021118 plant-derived protein Nutrition 0.000 title claims abstract description 23
- 238000000605 extraction Methods 0.000 title claims description 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 36
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 36
- 235000018102 proteins Nutrition 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 239000002253 acid Substances 0.000 claims abstract description 12
- 210000000582 semen Anatomy 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 240000008042 Zea mays Species 0.000 claims description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 11
- 235000005822 corn Nutrition 0.000 claims description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 9
- 235000012343 cottonseed oil Nutrition 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 6
- 244000105624 Arachis hypogaea Species 0.000 claims description 6
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 6
- 235000018262 Arachis monticola Nutrition 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 235000020232 peanut Nutrition 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 230000006920 protein precipitation Effects 0.000 claims description 4
- 239000002893 slag Substances 0.000 claims description 4
- 235000013311 vegetables Nutrition 0.000 claims description 3
- 240000002791 Brassica napus Species 0.000 claims description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 2
- 239000011260 aqueous acid Substances 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 235000009973 maize Nutrition 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 230000011218 segmentation Effects 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 abstract description 6
- 235000019698 starch Nutrition 0.000 abstract description 6
- 239000008107 starch Substances 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000002791 soaking Methods 0.000 abstract description 3
- 230000001376 precipitating effect Effects 0.000 abstract 2
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 150000002333 glycines Chemical class 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- 239000006228 supernatant Substances 0.000 description 6
- 235000013312 flour Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 235000019198 oils Nutrition 0.000 description 4
- 238000004062 sedimentation Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000006210 lotion Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 2
- 241000958526 Cuon alpinus Species 0.000 description 2
- 235000011941 Tilia x europaea Nutrition 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 239000004571 lime Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 108010076039 Polyproteins Proteins 0.000 description 1
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 229930000755 gossypol Natural products 0.000 description 1
- 229950005277 gossypol Drugs 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005201 scrubbing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009923 sugaring Methods 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a method for extracting plant protein and the application, which comprises soaking the raw materials in water, dissolving out water-soluble protein, adding alkaline calcium hypochlorite water solution, stirring to adjust the pH value to be alkaline, precipitating, removing residues, adjusting the pH value to be acidic by acid, precipitating the protein, and separating to obtain the protein. Can be used for extracting protein from semen Maydis, semen Arachidis Hypogaeae, semen gossypii, semen Brassicae campestris, semen glycines or protein-containing plant. A new product is developed for starch or starch sugar production plants. The method has the advantages of simple and reasonable process, wide application range and higher economic value and practical value.
Description
The present invention relates to from pulse family, vegetables seed or oil cake, wastewater of starch factory, similar waste water, extract the method and technology field of plant protein.
At present, be raw material with the W-Gum or be corn sugar refinery of raw material with the corn directly, all can not extract protein in this production process, make protein in slag, this is the huge waste to protein resource.The oil cake of oil crops such as peanut cake, soya-bean cake generally also can only be made feed, and cottonseed cake also can only be made organic fertilizer because of poisonous.In recent years, though have small-sized alkaline process or salt method proteinic with this extraction, protein separation is with a step settling process, because protein can not all leach, its recovery rate is lower, and wherein impure more, and purity is poor.
The extracting method and the purposes that the purpose of this invention is to provide a kind of plant protein, its method is simple and practical, the protein recovery rate is higher, can from corn refine sugar the residue and soya-bean cake, peanut cake, cottonseed cake in extract the protein that the mankind is had higher-value, improved the economic worth of agricultural-product greatly.
The present invention is achieved in that a kind of method of extracting plant protein, it is characterized in that the raw material that will contain plant protein is soaked in water, with the stripping aqueous soluble protein, add the alkaline Losantin aqueous solution then and stir the accent pH value and be alkalescence, after precipitation removes slag, transfer pH value to be acid with acid again, make the generation protein precipitation, separate promptly getting protein.
In order further to improve its production efficiency, shorten the production cycle, add alkaline Losantin and transfer pH value to be preferably 7.5-11, and/or adding acid transfers pH value preferably to 4-6.8 scopes.The purpose that adds the alkaline Losantin aqueous solution is that protein is fully leached, and the tart purpose that is of adding acid accent PH is to produce protein precipitation.Transferring to PH is in 4-6.8 scopes, and dosage makes it to separate out gradually.Transfer pH value in order to improve the best segmentation of its productivity, find out the different iso-electric points of protein, be beneficial to separating out in a large number of protein precipitation, iso-electric point is respectively PH6.8-6.5, and PH5.4-4.5 o'clock, its speed of separating out was the highest.
Sour regulator solution hydrochloric acid, sulfuric acid or the acetic acid etc. of above-mentioned adding, the most handy hydrochloric acid uses more convenient when its concentration is 5-15%.
The above-mentioned described raw material that contains plant protein is a Semen Maydis powder, maize germ cake, peanut cake, cottonseed cake, rapeseed cake, soya-bean cake (or slag), and corn sugaring residue or other contain the residue raffinate similar raw materials such as (as grain distillerys) of plant protein.
The aqueous solution of above-mentioned alkaline Losantin is by NaOH and/or Na
2CO
3Deng and Losantin ((CaCl)
24H
2O) and water form; Typical concentrations NaOH0.4-1%, Losantin 0.4-1%, more convenient, its add-on is complied with the pH value of being transferred and is helped protein settling and separates out and be as the criterion.The immersion water of raw material, the alkaline Losantin aqueous solution or aqueous acid water can be used general water purification, preferably use deionized water.
Method for extracting proteins of the present invention is easy rationally, the productivity height, proteinic recovery rate is also high, be suitable for industrial production, reduced investment, high efficiency, not only can be used for producing in the production process of W-Gum, primverose, and can be used for from peanut, cottonseed, soybean, vegetable seed or other contain the plant of plant protein, extracting plant protein, simultaneously, proteinic purity and whiteness improve a lot.The W-Gum that adopts the present invention to produce can reach senior (especially) purified starch level, makes the quality upgrading of starch product.Because Losantin has bleaching and germicidal action, so starch can prevent that living contaminants is rotten in producing.The more important thing is the economic worth multiplication that can make corn or oil cake etc.
The present invention is described in further detail below in conjunction with embodiment, but not as a limitation of the invention.
EXAMPLE l:
L, raw material
1. commercially available corn flour (crossing 28 mesh sieves) 1000g;
2. sodium hydroxide (NaOH): analytical pure, consumption are charging capacity 0.5%, meter 5g;
3. Losantin (floating smart sheet): (efficient content 28% converts available chlorine content 57%: should add and float smart sheet 0.25%, add 2.45g in fact by chlorinated lime.
2, formulations prepared from solutions:
Sodium hydroxide and Losantin are used (each 20ml) deionized water dissolving in right amount respectively; Before using two liquid are mixed.
3, albumen leaching operation:
With the full powder 1000g of 3000-4000ml deionized water soaking corn, to stir 2-4 hours, actual stirring is more than 4 hours; Under agitation slowly drip above-mentioned vat liquor, add the back and continue to stir 3-4 hours flour standing sedimentations after 5 hours then, siphon (or emitting) supernatant liquor, then wash 4 red dog flours again and be washed till neutrality, sedimentation 6 hours is fully stirred in each washing behind the adding deionized water, emit supernatant liquor, preceding secondary cleaning water can be merged to vat liquor for extracting protein, twice washing lotion in back can be made soak solution and use.
4, protein is separated out reclaimer operation:
This experiment under agitation slowly drips below the pH value to 6.8 with medical dilute hydrochloric acid (10%), promptly there is more protein to separate out, stop to drip, wait not have when separating out, drip again, about PH6, have more protein to separate out, stop dripping dilute acid liquid, albumen adds between the acid solution PH4.5-5.5 after condensing rapidly again, has than polyprotein again and separates out.After treating protein settling, emit supernatant liquor, but the usefulness of soak solution or washing lotion is made in this liquid backspace.
Emit after the supernatant liquor, protein is drenched do, through air-dry one-tenth block (faint yellow), industrial production should get protein 108g through pulverizing.
Embodiment 2:
1, raw material:
1. (asperities: 28 orders) 80kg feeds intake 2 times for corn powder or flour;
2. sodium hydroxide (NaOH): analytical pure consumption 0.5% * 80kg, consumption: 400g (secondary);
3. Losantin (Ca (ClO)
24H
2O): food chlorinated lime available chlorine content 28%, consumption is a charging capacity 0.5%, each consumption 400g;
4. use 2 liters of deionized water dissolvings respectively, mix before using, Losantin keeps in Dark Place.
2, protein lixiviate operation:
Corn powder 80kg+400 rises the deionized water soaking and stirring, soaks 4 hours, and standing sedimentation 6 hours is emitted supernatant liquor, adds deionized water 200-300 again and rises washing, emits supernatant liquor after the sedimentation, and repetitive scrubbing 4 red dog flours are washed till neutrality.Can be with preceding 2 washing lotion united extraction protein.
Secondary 160kg corn altogether feeds intake.
3, protein is separated out operation: the same.
Amount to humid protein 43kg, amount to and do solid protein 15.64kg, albumen yield 9.77%.
Embodiment 3:
With the cottonseed cake is raw material, the 60kg that feeds intake * 2 time.
It is identical with corn powder that extracting technology and albumen are separated out technology, protein yield 37.5%; Color (pulverizing the back protein powder): khaki color powder.
Hebei Prov. Health and Epidemic Prevention Station is 0.036% to the content assay of objectionable constituent free gossypol in the cottonseed protein that is extracted, the permission content of protein standard (0.06%), Nippon Standard (0.045%) and the USS (0.04%) that provides well below albumen consultation service of Food and Argriculture OrganizationFAO.
Claims (10)
1, a kind of method of extracting plant protein, it is characterized in that the raw material that will contain plant protein is soaked in water, with the stripping aqueous soluble protein, add the alkaline Losantin aqueous solution then and stir the accent pH value and be alkalescence, after precipitation removes slag, transfer pH value to be acid with acid again, make the generation protein precipitation, separate promptly getting protein.
2, the method for extraction plant protein according to claim 1, it is characterized in that adding alkaline Losantin accent pH value is 7.5-11, and/or adds acid accent pH value to 4-6.8.
3, the method for extraction plant protein according to claim 2, it is characterized in that adding acid, to transfer pH value be 4-6.8, for pH value is transferred in segmentation, to find out proteinic different iso-electric point, is beneficial to sedimentaryly fold in a large number.
4, the method for extraction plant protein according to claim 3 is characterized in that iso-electric point is respectively pH value 6.8-6.5, PH5.4-4.5.
5, the method for extraction plant protein according to claim 1 is characterized in that described acid hydrochloric acid, sulfuric acid or acetic acid.
6, the method for extraction plant protein according to claim 5 is characterized in that concentration of hydrochloric acid is 5-15%.
7, the method for extraction plant protein according to claim 1 is characterized in that described raw material is Semen Maydis powder, maize germ cake, peanut cake, cottonseed cake, rapeseed cake, soya-bean cake or the raffinate that contains plant protein.
8, according to the method for claim 1,2,3,4,5,6 or 7 described extraction plant proteins, the concentration that it is characterized in that the alkaline Losantin aqueous solution is NaOH0.4-1%, Losantin 0.4-1%.
9, the method for extraction plant protein according to claim 8 is characterized in that the immersion water of raw material, the alkaline Losantin aqueous solution or aqueous acid deionized water.
10,, it is characterized in that being used for extracting plant protein from corn, peanut, cottonseed, vegetable seed, soybean or other proteinaceous plants according to the purposes of the described method of claim 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94116992A CN1064370C (en) | 1994-10-22 | 1994-10-22 | Extraction method and application of plant protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94116992A CN1064370C (en) | 1994-10-22 | 1994-10-22 | Extraction method and application of plant protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1121926A true CN1121926A (en) | 1996-05-08 |
| CN1064370C CN1064370C (en) | 2001-04-11 |
Family
ID=5038209
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN94116992A Expired - Fee Related CN1064370C (en) | 1994-10-22 | 1994-10-22 | Extraction method and application of plant protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1064370C (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433020A (en) * | 2011-12-21 | 2012-05-02 | 晨光生物科技集团股份有限公司 | Method for preparing natural brown pigment from cotton seeds |
| CN102659233A (en) * | 2012-05-04 | 2012-09-12 | 泰祥集团技术开发有限公司 | Method for removing proteins in protein-enriched wastewater |
| CN103418160A (en) * | 2012-05-18 | 2013-12-04 | 华中农业大学 | Extracting liquid for extracting plant polyphenol and extracting method |
| CN103535506A (en) * | 2013-09-30 | 2014-01-29 | 宁国市沙埠粮油加工厂 | Method for extracting protein from rape seed cake |
| CN106417885A (en) * | 2016-10-11 | 2017-02-22 | 重庆多典食品有限公司 | Extracting method of plant proteins |
| CN108244329A (en) * | 2018-02-26 | 2018-07-06 | 山东师范大学 | A kind of preparation process of quinoa germ protein powder |
| CN110003307A (en) * | 2019-04-29 | 2019-07-12 | 中国海洋大学 | A kind of method of Protein Recovery in proteinic wastewater |
| FR3085680A1 (en) | 2018-09-06 | 2020-03-13 | Air Liquide France Industrie | IMPROVING PROTEIN RECOVERY BY CO2 ACIDIFICATION |
| CN114853528A (en) * | 2022-03-07 | 2022-08-05 | 江苏海洋大学 | Method for producing foliar fertilizer containing organic matters by using corn soaking solution |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE904225A (en) * | 1985-02-14 | 1986-05-29 | Fuji Oil Co Ltd | PROTEIN FRACTIONATION PROCESS. |
| IT1229216B (en) * | 1989-03-31 | 1991-07-25 | Italfarmaco Spa | PROTEINS INHIBIT RIBOSOMES AND THEIR DERIVATIVES. |
| CN1089618A (en) * | 1993-01-19 | 1994-07-20 | 济南七星技术服务公司 | To produce the method for vegetable-protein in the cottonseed cake |
-
1994
- 1994-10-22 CN CN94116992A patent/CN1064370C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433020A (en) * | 2011-12-21 | 2012-05-02 | 晨光生物科技集团股份有限公司 | Method for preparing natural brown pigment from cotton seeds |
| CN102433020B (en) * | 2011-12-21 | 2013-11-06 | 晨光生物科技集团股份有限公司 | Method for preparing natural brown pigment from cotton seeds |
| CN102659233A (en) * | 2012-05-04 | 2012-09-12 | 泰祥集团技术开发有限公司 | Method for removing proteins in protein-enriched wastewater |
| CN103418160A (en) * | 2012-05-18 | 2013-12-04 | 华中农业大学 | Extracting liquid for extracting plant polyphenol and extracting method |
| CN103418160B (en) * | 2012-05-18 | 2015-08-26 | 华中农业大学 | A kind of extract and extracting method extracting plant polyphenol |
| CN103535506A (en) * | 2013-09-30 | 2014-01-29 | 宁国市沙埠粮油加工厂 | Method for extracting protein from rape seed cake |
| CN106417885A (en) * | 2016-10-11 | 2017-02-22 | 重庆多典食品有限公司 | Extracting method of plant proteins |
| CN108244329A (en) * | 2018-02-26 | 2018-07-06 | 山东师范大学 | A kind of preparation process of quinoa germ protein powder |
| FR3085680A1 (en) | 2018-09-06 | 2020-03-13 | Air Liquide France Industrie | IMPROVING PROTEIN RECOVERY BY CO2 ACIDIFICATION |
| CN110003307A (en) * | 2019-04-29 | 2019-07-12 | 中国海洋大学 | A kind of method of Protein Recovery in proteinic wastewater |
| CN114853528A (en) * | 2022-03-07 | 2022-08-05 | 江苏海洋大学 | Method for producing foliar fertilizer containing organic matters by using corn soaking solution |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1064370C (en) | 2001-04-11 |
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