CN1121926A

CN1121926A - Process for extraction of plant protein and use thereof

Info

Publication number: CN1121926A
Application number: CN 94116992
Authority: CN
Inventors: 王崴
Original assignee: Individual
Current assignee: Individual
Priority date: 1994-10-22
Filing date: 1994-10-22
Publication date: 1996-05-08
Anticipated expiration: 2014-10-22
Also published as: CN1064370C

Abstract

The process for extracting protein from corn, peanut, cotton seed, rape seed, soybean or other plant containing protein comprises soaking raw material in water for dissolving out water-soluble protein, adding alkaline aqueous solution of calcium hypochlorite while stirring to make pH value alkaline, precipitation for removing dregs, adding acid to make pH value acidic for the precipitation of protein, and separation.

Description

The extracting method of one kind of plant protein and purposes

The present invention relates to from pulse family, vegetables seed or oil cake, wastewater of starch factory, similar waste water, extract the method and technology field of plant protein.

At present, be raw material with the W-Gum or be corn sugar refinery of raw material with the corn directly, all can not extract protein in this production process, make protein in slag, this is the huge waste to protein resource.The oil cake of oil crops such as peanut cake, soya-bean cake generally also can only be made feed, and cottonseed cake also can only be made organic fertilizer because of poisonous.In recent years, though have small-sized alkaline process or salt method proteinic with this extraction, protein separation is with a step settling process, because protein can not all leach, its recovery rate is lower, and wherein impure more, and purity is poor.

The extracting method and the purposes that the purpose of this invention is to provide a kind of plant protein, its method is simple and practical, the protein recovery rate is higher, can from corn refine sugar the residue and soya-bean cake, peanut cake, cottonseed cake in extract the protein that the mankind is had higher-value, improved the economic worth of agricultural-product greatly.

The present invention is achieved in that a kind of method of extracting plant protein, it is characterized in that the raw material that will contain plant protein is soaked in water, with the stripping aqueous soluble protein, add the alkaline Losantin aqueous solution then and stir the accent pH value and be alkalescence, after precipitation removes slag, transfer pH value to be acid with acid again, make the generation protein precipitation, separate promptly getting protein.

In order further to improve its production efficiency, shorten the production cycle, add alkaline Losantin and transfer pH value to be preferably 7.5-11, and/or adding acid transfers pH value preferably to 4-6.8 scopes.The purpose that adds the alkaline Losantin aqueous solution is that protein is fully leached, and the tart purpose that is of adding acid accent PH is to produce protein precipitation.Transferring to PH is in 4-6.8 scopes, and dosage makes it to separate out gradually.Transfer pH value in order to improve the best segmentation of its productivity, find out the different iso-electric points of protein, be beneficial to separating out in a large number of protein precipitation, iso-electric point is respectively PH6.8-6.5, and PH5.4-4.5 o'clock, its speed of separating out was the highest.

Sour regulator solution hydrochloric acid, sulfuric acid or the acetic acid etc. of above-mentioned adding, the most handy hydrochloric acid uses more convenient when its concentration is 5-15%.

The above-mentioned described raw material that contains plant protein is a Semen Maydis powder, maize germ cake, peanut cake, cottonseed cake, rapeseed cake, soya-bean cake (or slag), and corn sugaring residue or other contain the residue raffinate similar raw materials such as (as grain distillerys) of plant protein.

The aqueous solution of above-mentioned alkaline Losantin is by NaOH and/or Na ₂CO ₃Deng and Losantin ((CaCl) ₂4H ₂O) and water form; Typical concentrations NaOH0.4-1%, Losantin 0.4-1%, more convenient, its add-on is complied with the pH value of being transferred and is helped protein settling and separates out and be as the criterion.The immersion water of raw material, the alkaline Losantin aqueous solution or aqueous acid water can be used general water purification, preferably use deionized water.

Method for extracting proteins of the present invention is easy rationally, the productivity height, proteinic recovery rate is also high, be suitable for industrial production, reduced investment, high efficiency, not only can be used for producing in the production process of W-Gum, primverose, and can be used for from peanut, cottonseed, soybean, vegetable seed or other contain the plant of plant protein, extracting plant protein, simultaneously, proteinic purity and whiteness improve a lot.The W-Gum that adopts the present invention to produce can reach senior (especially) purified starch level, makes the quality upgrading of starch product.Because Losantin has bleaching and germicidal action, so starch can prevent that living contaminants is rotten in producing.The more important thing is the economic worth multiplication that can make corn or oil cake etc.

The present invention is described in further detail below in conjunction with embodiment, but not as a limitation of the invention.

EXAMPLE l:

L, raw material

1. commercially available corn flour (crossing 28 mesh sieves) 1000g;

2. sodium hydroxide (NaOH): analytical pure, consumption are charging capacity 0.5%, meter 5g;

3. Losantin (floating smart sheet): (efficient content 28% converts available chlorine content 57%: should add and float smart sheet 0.25%, add 2.45g in fact by chlorinated lime.

2, formulations prepared from solutions:

Sodium hydroxide and Losantin are used (each 20ml) deionized water dissolving in right amount respectively; Before using two liquid are mixed.

3, albumen leaching operation:

With the full powder 1000g of 3000-4000ml deionized water soaking corn, to stir 2-4 hours, actual stirring is more than 4 hours; Under agitation slowly drip above-mentioned vat liquor, add the back and continue to stir 3-4 hours flour standing sedimentations after 5 hours then, siphon (or emitting) supernatant liquor, then wash 4 red dog flours again and be washed till neutrality, sedimentation 6 hours is fully stirred in each washing behind the adding deionized water, emit supernatant liquor, preceding secondary cleaning water can be merged to vat liquor for extracting protein, twice washing lotion in back can be made soak solution and use.

4, protein is separated out reclaimer operation:

This experiment under agitation slowly drips below the pH value to 6.8 with medical dilute hydrochloric acid (10%), promptly there is more protein to separate out, stop to drip, wait not have when separating out, drip again, about PH6, have more protein to separate out, stop dripping dilute acid liquid, albumen adds between the acid solution PH4.5-5.5 after condensing rapidly again, has than polyprotein again and separates out.After treating protein settling, emit supernatant liquor, but the usefulness of soak solution or washing lotion is made in this liquid backspace.

Emit after the supernatant liquor, protein is drenched do, through air-dry one-tenth block (faint yellow), industrial production should get protein 108g through pulverizing.

Embodiment 2:

1, raw material:

1. (asperities: 28 orders) 80kg feeds intake 2 times for corn powder or flour;

2. sodium hydroxide (NaOH): analytical pure consumption 0.5% * 80kg, consumption: 400g (secondary);

3. Losantin (Ca (ClO) ₂4H ₂O): food chlorinated lime available chlorine content 28%, consumption is a charging capacity 0.5%, each consumption 400g;

4. use 2 liters of deionized water dissolvings respectively, mix before using, Losantin keeps in Dark Place.

2, protein lixiviate operation:

Corn powder 80kg+400 rises the deionized water soaking and stirring, soaks 4 hours, and standing sedimentation 6 hours is emitted supernatant liquor, adds deionized water 200-300 again and rises washing, emits supernatant liquor after the sedimentation, and repetitive scrubbing 4 red dog flours are washed till neutrality.Can be with preceding 2 washing lotion united extraction protein.

Secondary 160kg corn altogether feeds intake.

3, protein is separated out operation: the same.

Amount to humid protein 43kg, amount to and do solid protein 15.64kg, albumen yield 9.77%.

Embodiment 3:

With the cottonseed cake is raw material, the 60kg that feeds intake * 2 time.

It is identical with corn powder that extracting technology and albumen are separated out technology, protein yield 37.5%; Color (pulverizing the back protein powder): khaki color powder.

Hebei Prov. Health and Epidemic Prevention Station is 0.036% to the content assay of objectionable constituent free gossypol in the cottonseed protein that is extracted, the permission content of protein standard (0.06%), Nippon Standard (0.045%) and the USS (0.04%) that provides well below albumen consultation service of Food and Argriculture OrganizationFAO.

Claims

1, a kind of method of extracting plant protein, it is characterized in that the raw material that will contain plant protein is soaked in water, with the stripping aqueous soluble protein, add the alkaline Losantin aqueous solution then and stir the accent pH value and be alkalescence, after precipitation removes slag, transfer pH value to be acid with acid again, make the generation protein precipitation, separate promptly getting protein.

2, the method for extraction plant protein according to claim 1, it is characterized in that adding alkaline Losantin accent pH value is 7.5-11, and/or adds acid accent pH value to 4-6.8.

3, the method for extraction plant protein according to claim 2, it is characterized in that adding acid, to transfer pH value be 4-6.8, for pH value is transferred in segmentation, to find out proteinic different iso-electric point, is beneficial to sedimentaryly fold in a large number.

4, the method for extraction plant protein according to claim 3 is characterized in that iso-electric point is respectively pH value 6.8-6.5, PH5.4-4.5.

5, the method for extraction plant protein according to claim 1 is characterized in that described acid hydrochloric acid, sulfuric acid or acetic acid.

6, the method for extraction plant protein according to claim 5 is characterized in that concentration of hydrochloric acid is 5-15%.

7, the method for extraction plant protein according to claim 1 is characterized in that described raw material is Semen Maydis powder, maize germ cake, peanut cake, cottonseed cake, rapeseed cake, soya-bean cake or the raffinate that contains plant protein.

8, according to the method for claim 1,2,3,4,5,6 or 7 described extraction plant proteins, the concentration that it is characterized in that the alkaline Losantin aqueous solution is NaOH0.4-1%, Losantin 0.4-1%.

9, the method for extraction plant protein according to claim 8 is characterized in that the immersion water of raw material, the alkaline Losantin aqueous solution or aqueous acid deionized water.

10,, it is characterized in that being used for extracting plant protein from corn, peanut, cottonseed, vegetable seed, soybean or other proteinaceous plants according to the purposes of the described method of claim 1-9.