CN1182593A

CN1182593A - Chondroitiln sulfate and extrating method

Info

Publication number: CN1182593A
Application number: CN96116057A
Authority: CN
Inventors: 王立杰; 赵佳民
Original assignee: Individual
Current assignee: Individual
Priority date: 1996-11-18
Filing date: 1996-11-18
Publication date: 1998-05-27

Abstract

The invented method for extracting chondroitin sulfate uses the animal's cartilage as raw material and the product is obtained through leaching, extracting, hydrochloride decomposition ,acid protein removing, purification precipitation and drying. Said method adopts dilute basicity-strong brine extracting new technological process and has no need of zymolysis and decolouring treatment. The product features clean white, and spongy, the yield is between 15-20% and up to the oral quality standards, besides it features also easy operation, low cost and short productive period.

Description

Chondroitin sulfate and extracting method thereof

The invention belongs to a kind of biochemical preparation, particularly be to utilize animal cartilage to make chondroitin sulfate and extracting method thereof that raw material extracts.

At present, the method of domestic extraction chondroitin sulfate, diluted alkaline-zymohydrolysis extracting method is roughly arranged, enzymolysis-resin extraction method, the dense salt extraction methods of diluted alkaline etc. are several, wherein diluted alkaline-zymohydrolysis extracting method is made adsorbent with alukalin and active carbon, though can remove remaining protein and polypeptide, but some chrondroitins also will be adsorbed, thereby influenced yield, alukalin will bring trouble and increase cost to production through handling in this method in addition, the terminal point of enzymolysis is difficult to control simultaneously, difficulty was very big when protein solution becomes after hydrolysis got the thickness filtration, in the enzymolysis one resin extraction method, adopted A mberlire IRA-933 type ion exchange resin, the resin price costliness, it is loaded down with trivial details to handle trouble, need be very uneconomical when particularly increasing output with a large amount of resins, in the dense salt extraction method of diluted alkaline, need the very big time except that acid albumen adjusting PH1-2 filters when output is big, the amino in the chondroitin sulfate will be disintegrated down has influenced yield and quality.

The objective of the invention is to overcome above-mentioned the deficiencies in the prior art, a kind of method of extracting chondroitin sulfate is provided, make the quality of its product good, cost is low, and production technology is simple.

The present invention is achieved in that

A kind of chondroitin sulfate is characterized in a kind of dissolving fully of extracting from animal cartilage, its optical rotation is-20 to-35 ℃, the white powder of the 24-30% of glucosamine content.

A kind of extracting method of chondroitin sulfate is:

(1) pure white clean broken cartilage is placed in the extraction pot, add concentration 3-3.5mal/l till sodium chloride solution makes the complete submergence of bone slag.Regulate PH12-13 with sodium hydroxide and stir extraction filtration in 10-15 hour, the same operation of filtering residue is brought up again once, back and 2 extracting solution.

(2) extracting solution is regulated PH to 7-8 with hydrochloric acid and is warmed up to 70-90 ℃ of maintenance 10-60 minute rapidly, cooled and filtered, and getting filtrate is that salt is separated liquid.

(3) salt is separated liquid, adds calcium chloride and filters to get filtrate.

(4) filtrate of process (3) adds hydrogen peroxide, transfers 15-25 hour after-filtration of PH8-10 oxidation, gets filtrate.

(5) filtrate of process (4) transfers PH6-7 to add 1 times of deionized water, adds 95% ethanol again, makes the concentration of alcohol reach 50-60%, deposits to clearly, and siphon removes supernatant, and precipitation drains, dewaters, dry chondroitin sulfate finished product.

Above-mentioned chondroitin sulfate, to separate the concentration that adds calcium chloride in the liquid be 1-5% to salt in the extracting method.

The concentration of the hydrogen peroxide that adds in the above-mentioned chondroitin sulfate extracting method is 1-3%.

Embodiment 1

1, pure white clean broken cartilage is placed in the extraction pot, the sodium chloride solution of liquid feeding degree 3mal/l makes till the complete submergence of bone slag, regulates PH12.5 stirring extraction filtration, the same operation of filtering residue in 10 hours with sodium hydroxide and brings up again once, merges 2 times extracting solution.

2, extracting solution is regulated PH to 7 with hydrochloric acid and is warmed up to 80 ℃ of maintenances 20 minutes rapidly, and it is that salt is separated liquid that cooled and filtered gets filtrate.

3, salt is separated liquid and is added 1.5% calcium chloride and filter to get filtrate.

4, the hydrogen peroxide of the filtrate of process (3) adding 2% transfers 20 hours after-filtration of PH9.5 oxidation to get filtrate.

5, the filtrate of process (4) transfers PH6.5 to add 1 times of deionized water, adds 95% ethanol again, make the concentration of alcohol reach 55% deposit to clear, siphon removes supernatant, precipitate, drain, dewater, dry chondroitin sulfate finished product.

Advantage of the present invention is a lot and since this technology be with the dense salt of diluted alkaline extract use again afterwards calcium chloride, Hydrogen peroxide precipitation, oxidation isolating protein, and decolour, the product yield height, the color and luster that obtain are clean In vain, powder is loose, meet oral quality standard, and production operation is easy, cost is lower, the cycle Short.

The finished product that adopts above method to obtain, its testing result is;

The concentration that adds calcium chloride in the extracting method of above-mentioned chondroitin sulfate should be that the concentration that 1-5% adds hydrogen peroxide should be 1-3%

Yield (for cartilage) 15%

The outward appearance colored powder

Solubility (2%H2O) is complete

Optical activity-20-125

Dry moisture＜10%

Protein＜3.5%

Heavy metal＜30ppm

Glucosamine content 24-30%

Bacterial number＜1000cfwlg

Pathogen exists

See that from above testing result product meets oral level index

Should note following problem when producing this product

1, to notice that the environment in whole workshop is clean and tidy, the staff will note health, prevent that product from making moist, and is subjected to germ contamination.

2, filtering in each operation is very importantly to prevent evaporating, emitting, dripping or leaking of liquid or gas phenomenon in operation, and accomplishes and filter rapidly.

Because this technology is to extract with the dense salt of diluted alkaline to use calcium chloride, hydrogen peroxide, precipitation oxidation isolating protein again afterwards, and decolours, so the product yield height that obtains, color and luster is pure white, and powder rice is loose, and it is easy to meet oral quality standard and production operation, cost is lower, and the cycle is short.

Claims

1, a kind of chondroitin sulfate is characterized in that: be dissolving fully of extracting from animal cartilage, optical rotation-20--35 ℃, glucosamine content is the white powder of 24-30%.

2, the extracting method of the described chondroitin sulfate of a kind of claim 1 is characterized in that:

(1) pure white clean broken cartilage is placed in the extraction pot, the sodium chloride solution that adds concentration 3-3.5mal/l makes till the complete submergence of bone slag, bring up again 1 time with sodium hydroxide adjusting PH12-13 stirring extraction 10-15 hour, filtration, the same operation of filtering residue, merge 2 times extracting solution;

(2) extracting solution is regulated PH to 7-8 with hydrochloric acid and is warmed up to 70-90 ℃ of maintenance 10-60 minute rapidly, and it is that salt is separated liquid that cooled and filtered gets filtrate;

(3) salt is separated liquid and is added calcium chloride and filter to get filtrate;

(4) filtrate of process (3) adds hydrogen peroxide, transfers 15 hours after-filtration of PH8.5-10 chlorination to get filtrate;

(5) filtrate of process (4) transfers PH6-7 to add 1 times of deionized water to add 95% ethanol again, makes the concentration of alcohol reach 50-60% and deposits to clearly, and siphon removes that the supernatant precipitation is drained, dewatered, dry chondroitin sulfate finished product.

3, chondroitin sulfate production method according to claim 2 is characterized in that it should be 1-5% that salt is separated the concentration that liquid adds calcium chloride.

4, chondroitin sulfate production method according to claim 2 is characterized in that the concentration of the hydrogen peroxide that adds is 1-3%.