CN103570799A - Method for comprehensively utilizing soft bones of livestock and poultry - Google Patents
Method for comprehensively utilizing soft bones of livestock and poultry Download PDFInfo
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- CN103570799A CN103570799A CN201310517184.4A CN201310517184A CN103570799A CN 103570799 A CN103570799 A CN 103570799A CN 201310517184 A CN201310517184 A CN 201310517184A CN 103570799 A CN103570799 A CN 103570799A
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Abstract
The invention discloses a method for comprehensively utilizing soft bones of livestock and poultry. According to the method, the soft bones of various livestock and poultry are crushed, then are added with a 10% sodium hydroxide solution which is twice the weight of the crushed bones, and are heated in water bath to 40-60 DEG C, soaked for 10-12 hours and filtered; the filtrate is regulated with sulfuric acid until the pH value is 3-4and stands for one hour and then is filtered; filter residues are washed repeatedly with clean water till neutrality, and are dried and crushed below 60 DEG C to obtain bone protein; the filtrate is regulated to neutrality by using sodium hydroxide, is added with proteinase accounting for 2wt% of the crushed bone, is heated in water bath to 60 DEG C, hydrolyzes for 10 hours by keeping the temperature unchanged and is filtered, the filtrate is vacuum-evaporated under the conditions of 0.5 MPa and 60 DEG C, the dry substances obtained after evaporation are washed for 2-4 times with 90% ethanol and filtered to obtain chondroitin sulfate. The process of alkali-solution and acid-isolation is used in the method so that chondroitin in soft bones is fully soaked, and the yield and quality of chondroitin sulfate are greatly improved.
Description
Technical field
The present invention relates to a kind of animal bone and utilize method, be specifically related to a kind of livestock and poultry cartilage method of comprehensive utilization.
Background technology
Livestock and poultry cartilage is a kind of large tankage in slaughterhouse, and conventional situation is all processed as fodder additives, and added value is extremely low.And in animal cartilage, contain a large amount of animal proteinums and chondroitin sulfate, chondroitin sulfate wherein has reducing blood-fat, anticoagulation, anti-inflammatory and the physiologically active such as antitumor, can be used for clinically the sclerosis of prevention of arterial atherosclerotic, improves immunity of organisms, treatment rheumatosis and ephritis and the dysacousis that caused by Streptomycin sulphate.Chondroitin sulfate is combined and is can be used for treating tetter with metal ions such as copper, react with Bismuth trinitrate or aluminium hydroxide the complex compound obtaining and can be used for treating peptide ulceration, the derivative generating with benzoic ether, lignocaine or bupivacaine effect can be used as narcotic.As foodstuff additive, chondroitin sulfate can be used for emulsification, moisturizing and the dispelling abnormal flavor of food.In makeup, add chondroitin sulfate, the cellular metabolism of adjustable skin, promotes the absorption of nutrition, keeps the moisture of skin and improves hair quality.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, and a kind of new livestock and poultry cartilage method of comprehensive utilization is provided.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of livestock and poultry cartilage method of comprehensive utilization, is characterized in: various livestock and poultry cartilages are pulverized, added 10% sodium hydroxide solution of 2 times of broken bone weights, heating in water bath, to 40-60 ℃, soaks 10-12 hour, filters, with sulfuric acid, regulate filtrate pH value to 3-4, standing 1 hour, filter.Filter residue is rinsed to neutrality repeatedly with clear water, 60 ℃ of following oven dry, pulverizing, be bone protein.Filtrate is adjusted to neutrality with sodium hydroxide, adds 2% proteolytic enzyme of broken bone weight, heating in water bath to 60 ℃, keep temperature hydrolysis 10 hours, filter, by filtrate vacuum-evaporation at 0.5MPa, 60 ℃, by 90% washing with alcohol 2-4 time for the dry-matter after evaporation, filtration, is chondroitin sulfate.
Compared with prior art, the present invention has used the processing method of alkali extraction and acid precipitation, and the chrondroitin in cartilage is fully leached, thereby has greatly improved the yield and quality of chondroitin sulfate.
Embodiment
Below further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.
Embodiment 1, a cartilage method of comprehensive utilization, rejects impurity by various livestock and poultry cartilages, with clear water, cleans, pulverizes, get 10 kilograms, broken bone, add 20 kilograms of 10% sodium hydroxide solutions, heating in water bath to 60 ℃, soaks 12 hours, filter, with sulfuric acid, regulate filtrate pH value to 3.5, standing 1 hour, filter.Filter residue is rinsed to neutrality repeatedly with clear water, and oven dry at 50 ℃ of temperature, pulverizing are bone protein.Filtrate is adjusted to neutrality with sodium hydroxide, adds 0.2 kilogram of proteolytic enzyme of broken bone weight, heating in water bath to 60 ℃, keep temperature hydrolysis 10 hours, filter, by filtrate vacuum-evaporation at 0.5MPa, 60 ℃, by 90% washing with alcohol 4 times for the dry-matter after evaporation, filtration, is chondroitin sulfate.
Claims (6)
1. a livestock and poultry cartilage method of comprehensive utilization, is characterized in that raw materials used livestock and poultry cartilage, through cleaning, pulverizing, alkali soak, filtration, sour tune, precipitation, filtration, albumen oven dry, enzymolysis, distillation, washing, oven dry.
2. a kind of livestock and poultry cartilage method of comprehensive utilization according to claim 1, is characterized in that it is 10% sodium hydroxide solution with 2 times of broken bone weights that described alkali soaks, and heating in water bath, to 40-60 ℃, soaks 10-12 hour.
3. a kind of livestock and poultry cartilage method of comprehensive utilization according to claim 1, is characterized in that it is to regulate filtrate pH value to 3-4 with sulfuric acid that described acid is adjusted, standing 1 hour.
4. a kind of livestock and poultry cartilage method of comprehensive utilization according to claim 1, is characterized in that described enzymolysis is 2% proteolytic enzyme with broken bone weight, and filtrate water-bath is heated to 60 ℃, keeps temperature hydrolysis 10 hours.
5. a kind of livestock and poultry cartilage method of comprehensive utilization according to claim 1, is characterized in that described distillation is by filtrate vacuum-evaporation at 0.5MPa, 60 ℃.
6. a kind of livestock and poultry cartilage method of comprehensive utilization according to claim 1, is characterized in that described washing is by 90% washing with alcohol 2-4 time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310517184.4A CN103570799A (en) | 2013-10-29 | 2013-10-29 | Method for comprehensively utilizing soft bones of livestock and poultry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310517184.4A CN103570799A (en) | 2013-10-29 | 2013-10-29 | Method for comprehensively utilizing soft bones of livestock and poultry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103570799A true CN103570799A (en) | 2014-02-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310517184.4A Pending CN103570799A (en) | 2013-10-29 | 2013-10-29 | Method for comprehensively utilizing soft bones of livestock and poultry |
Country Status (1)
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| CN (1) | CN103570799A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017046A (en) * | 2014-06-23 | 2014-09-03 | 四川省中医药科学院 | Bone protein and extraction method thereof |
Citations (5)
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|---|---|---|---|---|
| CN1182593A (en) * | 1996-11-18 | 1998-05-27 | 王立杰 | Chondroitiln sulfate and extrating method |
| CN1296979A (en) * | 1999-11-17 | 2001-05-30 | 许累峰 | Chondroitin sulfate and production method thereof |
| CN102453108A (en) * | 2010-10-19 | 2012-05-16 | 河南双汇投资发展股份有限公司 | Combined production method for processing chondroitin sulfate and protein powder with bird skeletons as raw material |
| CN102898541A (en) * | 2012-10-19 | 2013-01-30 | 宜昌湘宜水产品有限公司 | Method for preparing fish bone chondroitin sulfate |
| CN103319628A (en) * | 2013-07-02 | 2013-09-25 | 淮北市恒通生物科技有限公司 | Method for preparing chondroitin sulfate by virtue of ultrahigh-pressure micro-jet ultrafiltration |
-
2013
- 2013-10-29 CN CN201310517184.4A patent/CN103570799A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1182593A (en) * | 1996-11-18 | 1998-05-27 | 王立杰 | Chondroitiln sulfate and extrating method |
| CN1296979A (en) * | 1999-11-17 | 2001-05-30 | 许累峰 | Chondroitin sulfate and production method thereof |
| CN102453108A (en) * | 2010-10-19 | 2012-05-16 | 河南双汇投资发展股份有限公司 | Combined production method for processing chondroitin sulfate and protein powder with bird skeletons as raw material |
| CN102898541A (en) * | 2012-10-19 | 2013-01-30 | 宜昌湘宜水产品有限公司 | Method for preparing fish bone chondroitin sulfate |
| CN103319628A (en) * | 2013-07-02 | 2013-09-25 | 淮北市恒通生物科技有限公司 | Method for preparing chondroitin sulfate by virtue of ultrahigh-pressure micro-jet ultrafiltration |
Non-Patent Citations (2)
| Title |
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| 夏未铭等: "硫酸软骨素粉制备方法的改进", 《陕西师范大学学报(自然科学版)》 * |
| 陈红丽等: "硫酸软骨素提取方法研究", 《河南科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017046A (en) * | 2014-06-23 | 2014-09-03 | 四川省中医药科学院 | Bone protein and extraction method thereof |
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Application publication date: 20140212 |