CN1167768A - Method for extracting phytin from rice bran - Google Patents
Method for extracting phytin from rice bran Download PDFInfo
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- CN1167768A CN1167768A CN 97109805 CN97109805A CN1167768A CN 1167768 A CN1167768 A CN 1167768A CN 97109805 CN97109805 CN 97109805 CN 97109805 A CN97109805 A CN 97109805A CN 1167768 A CN1167768 A CN 1167768A
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- rice bran
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- fland
- luxuriant
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Abstract
A process for extracting phytine from rice bran includes soaking the rice bran in water in the condition of protein isoelectric point PI in a ratio of solid to liquid that is 1:8-12 at 10-50 deg. C for 2-72 hr while intermittent stirring, filtering the supernatant neutralizing with 30% NaOH until pH=9-10, precipitating filtering, washing with clean water and natural drying to obtain white phytine,and features simplified technology, low cost and high quality of product. Obtained phytine is used to prepare phytic acid and inositol.
Description
The present invention relates to a kind of solvent-extracted isolation technique, B01D 11/00 class is particularly extracted the novel method in luxuriant and rich with fragrance spit of fland from rice bran.
The rice bran wide material sources except that containing nutrition such as rich in protein, also contain 6～7% phytic acid in the rice bran, and it is generally with phytin double salt form A
6H
6O
24P
6Ca
4MgNa
2Exist, be called Fei Ting (phytin).Phosphorus wherein is difficult to be absorbed by animal, and because phytic acid has very strong complex ability, it forms mixture with metal ion and protein, can reduce micro-and proteinic biological value, after extracting luxuriant and rich with fragrance spit of fland by rice bran, increased in the rice bran effectively nutrition balance on the one hand; Luxuriant and rich with fragrance on the other hand spit of fland is medicine, industrial chemicals, and very large economy value is arranged, so, extract luxuriant and rich with fragrance spit of fland from rice bran, as feed, be the optimal selection that rationally utilizes the rice bran resource again.
Extracted the technology in luxuriant and rich with fragrance spit of fland from rice bran in the past, routine U.S. Pat 4070422, the processing condition of＜optimization luxuriant and rich with fragrance spit of fland preparation 〉=(author: few Zhou Kun, Zhou Houxu, Yang Fengmei. West China pharmaceutical journal .1995; 10 (2); All be to soak with diluted acid 96-9), acid soluble material in the raw material also partly enters in the pickling liquor as organic impuritys such as protein, starch, carbohydrates thereupon like this, and acid soak liquid comes down to the mixed solution of phytic acid and other impurity.The Fei Ting that contains impurity such as protein that obtains thus removes to produce phytic acid or inositol, can make subsequent handling increase load, reduces quality product.Remove deproteinize crucial must the separation.
The objective of the invention is, solve the rice bran acid soak, cause the Fei Ting of its extraction to contain the impurity of acid solution, make the low problem of its purity, a kind of work simplification is provided, what production cost was low extracts the novel method in luxuriant and rich with fragrance spit of fland by rice bran.
For realizing the purpose of foregoing invention, technical scheme of the present invention is as follows:
The present invention adopts under the condition of isoelectric points of proteins, leaches phytate, utilizes iso-electric point to destroy the protein colloid, and most of protein is removed with regard to separated at leaching process.
Extract the method in luxuriant and rich with fragrance spit of fland by rice bran, it is characterized in that: processing step is as follows successively:
A. soak, separate: rice bran is put in the container and is soaked under the condition of its isoelectric points of proteins PI, solid-to-liquid ratio S: L=1: 8～12, and soak time was at 2～72 hours, and temperature is done during this intermittently to stir to get final product between 10～50 ℃ slightly, and clear liquor filters then;
B. neutralization precipitation, filtration: the luxuriant and rich with fragrance spit of fland filtrate that will leach is neutralized to PH=9-10 with 30%NaOH, carries out sedimentation and filtration;
C. washing, dry, finished product: the Fei Ting behind the sedimentation and filtration, again with clear water washing, seasoning then, the Fei Ting that gets final product whitely.
The preferred ratio of rice bran and water is S: L=1 among the present invention: 8; The span of isoelectric points of proteins PI is in PH=5 ± 0.5; In rice bran cake, extract in the processing step in luxuriant and rich with fragrance spit of fland, need earlier rice bran cake to be pulverized 40 mesh sieves.
The luxuriant and rich with fragrance spit of fland of extraction of the present invention Selection of technological conditions rationale:
The relation of luxuriant and rich with fragrance spit of fland in the rice bran and protein solubility rate pH value.Experimental result shows: when iso-electric point PI (PH=5 ± 0.5), and luxuriant and rich with fragrance spit of fland solubility rate maximum (＞95%), and proteinic solubility rate minimum can be removed 85～90% protein.
Extraction time more than 2 hours to 72 hours, luxuriant and rich with fragrance spit of fland leaching yield all reaches more than 95%.Solid-to-liquid ratio S: L is little, and adsorbent solution amount relative proportion is big in the wet rice bran leached mud, the washing and filtering trouble.S: L is big, though solubility rate is higher, filtration yield is big, and strength of solution is low, and select moderate 8: L=1: 8～12 are advisable.
Though stir the concentration gradient that helps eliminating feed liquid, then, vigorous stirring can leach more impurity, make very difficulty of filtration, increase workload for the subsequent handling removal of impurities.The general static immersing that adopts is done intermittently stirring slightly and is got final product, and has both saved power consumption, and clear liquor is easy to again filter.
Temperature influences between 10～50 ℃ not quite, and heating promptly increases energy consumption, can cause phytic acid degraded loss again, adopts normal temperature and pressure to leach and gets final product.
Neutralization precipitation, my door adopts NaOH% to make precipitation agent, and the deposition rate in luxuriant and rich with fragrance spit of fland raises with PH and increases.When PH=9～10, luxuriant and rich with fragrance spit of fland deposition rate adds more alkali lye greater than 95%, and luxuriant and rich with fragrance spit of fland deposition rate increases little, and the color of solution is deepened greatly, and is unfavorable to decolouring.
The principle of described isoelectric points of proteins PI:
Protein is the polymer substance that is formed by connecting by peptide bond by many amino acid.Peptide bond
Form by an amino acid whose carboxyl and another amino acid whose amino dehydrating condensation.
But the peptide bond two ends still have amino and carboxyl, so protein is the same with the acid of nitrogen base, all are amphotericeledrolyte, and positively charged in acidic solution is electronegative in basic solution, and when iso-electric point PI, protein is with positive and negative electricity to equate, is electric neutrality.
P-Protein represents protein molecule
Alkalescence negative ion PH＞PI dipole ion PH=PI acidity. positive ion PH＜PI
Under acid and alkaline condition, protein is positively charged and negative charge respectively, mutually exclusive, be difficult for cohesion and precipitate, the protein particulate diameter is between 0.1～0.001 M, be and exist in the solution colloid-stabilisedly, and near iso-electric point, the protein net charge is minimum, runs foul of each other easily and coacervating large particle and precipitating, utilizing iso-electric point is to destroy colloid, a main method of precipitating proteins
The beneficial effect that the present invention is compared with prior art had is as follows:
The present invention is to adopt under the isoelectric points of proteins condition to soak rice bran with different in the past new breakthrough point, make the phytate dissolving enter solution, at iso-electric point PI, it is neutral that protein is, make it run foul of each other coacervating large particle and precipitate, so both simplified technology, saved the soda acid consumption, reduce cost, improved luxuriant and rich with fragrance spit of fland quality again. with the Fei Ting that this method obtains, its yield is more than 90%, purity＞greater than 95%, be suitable for as production phytic acid and the raw material of producing inositol, and protein resource is fully used in the rice bran, behind the luxuriant and rich with fragrance spit of fland of immersion extraction is the high-quality feed that contains high protein.
Specific embodiments of the invention are as follows:
Embodiment 1
Get rice bran 1Kg, add water, solid-to-liquid ratio S: L=1: 10, soaked 2 hours, to do slightly intermittently to stir, clear liquor filters, and filtrate is neutralized to PH=9～10 with 30%NaCH, sedimentation and filtration, the clear water washing dehydrates, and can obtain the Fei Ting of white.
Embodiment 2
Extracting degreasing rice bran 50g is soaked in water solid-to-liquid ratio S: L=1: 8, and soak time is 3 hours, obtain the rice bran leach liquor after filtering, add the 30NaOH neutralization again, make its PH=8～9, after leaving standstill, filter out throw out, add clear water washing after, dehydrate, can obtain Fei Ting.
Embodiment 3
After the rice bran cake pulverizing,, be soaked in water 5 hours its solid-to-liquid ratio S: L=1 after 40 mesh sieves: 10, filter the back leach liquor, add the 30NaOH neutralization again, make its PH=8～10, leave standstill after-filtration, after the clear water washing, dehydrate, can obtain Fei Ting.
Claims (4)
1, a kind ofly extract the novel method in luxuriant and rich with fragrance spit of fland, it is characterized in that processing step is as follows successively by rice bran:
A. soak, separate: rice bran is put in the container and is soaked in water solid-to-liquid ratio S: L=1 under the condition of its isoelectric points of proteins PI: 8～12, and soak time was at 2～72 hours, and temperature is done during this intermittently to stir between 10～50 ℃ slightly, and clear liquor filters then;
B. neutralization precipitation, filtration: the luxuriant and rich with fragrance spit of fland filtrate that will leach is neutralized to PH=9-10 with 30%NaOH, carries out sedimentation and filtration;
C. washing, dry, finished product: the Fei Ting behind the sedimentation and filtration is washed again seasoning then, the Fei Ting that gets final product whitely with clear water.
2, according to claim 1ly extract the novel method in luxuriant and rich with fragrance spit of fland by rice bran, it is characterized in that: the preferred ratio of rice bran and water is S: L=1: 8.
3, according to claim 1ly extract the novel method in luxuriant and rich with fragrance spit of fland by rice bran, it is characterized in that: the span of isoelectric points of proteins PI is in PH=5 ± 0.5.
4, according to claim 1ly extract the novel method in luxuriant and rich with fragrance spit of fland, it is characterized in that: in rice bran cake, extract in the processing step in luxuriant and rich with fragrance spit of fland, earlier rice bran cake was pulverized 40 orders by rice bran.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97109805 CN1167768A (en) | 1997-04-12 | 1997-04-12 | Method for extracting phytin from rice bran |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97109805 CN1167768A (en) | 1997-04-12 | 1997-04-12 | Method for extracting phytin from rice bran |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1167768A true CN1167768A (en) | 1997-12-17 |
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|---|---|---|---|
| CN 97109805 Pending CN1167768A (en) | 1997-04-12 | 1997-04-12 | Method for extracting phytin from rice bran |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107071C (en) * | 2000-10-30 | 2003-04-30 | 李国荣 | Process for preparing phytic acid |
| WO2003053162A1 (en) | 2001-12-20 | 2003-07-03 | Nippon Suisan Kaisha, Ltd. | Aqueous extract of rice bran and utilization thereof in additives for ground fish meat products |
| CN101602777B (en) * | 2009-07-03 | 2011-09-14 | 安徽丰原生物化学股份有限公司 | Method for extracting phytic acid |
| CN102690173A (en) * | 2012-06-13 | 2012-09-26 | 吉林农业大学 | Method for preparing inositol by Fe2(SO4)3/gamma-Al2O3 solid acid catalysis |
-
1997
- 1997-04-12 CN CN 97109805 patent/CN1167768A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107071C (en) * | 2000-10-30 | 2003-04-30 | 李国荣 | Process for preparing phytic acid |
| WO2003053162A1 (en) | 2001-12-20 | 2003-07-03 | Nippon Suisan Kaisha, Ltd. | Aqueous extract of rice bran and utilization thereof in additives for ground fish meat products |
| EP1457119A1 (en) * | 2001-12-20 | 2004-09-15 | Nippon Suisan Kaisha, Ltd. | Aqueous extract of rice bran and utilization thereof in additives for ground fish meat products |
| EP1457119A4 (en) * | 2001-12-20 | 2010-07-21 | Nippon Suisan Kaisha Ltd | Aqueous extract of rice bran and utilization thereof in additives for ground fish meat products |
| CN101602777B (en) * | 2009-07-03 | 2011-09-14 | 安徽丰原生物化学股份有限公司 | Method for extracting phytic acid |
| CN102690173A (en) * | 2012-06-13 | 2012-09-26 | 吉林农业大学 | Method for preparing inositol by Fe2(SO4)3/gamma-Al2O3 solid acid catalysis |
| CN102690173B (en) * | 2012-06-13 | 2015-02-18 | 吉林农业大学 | Method for preparing inositol by Fe2(SO4)3/gamma-Al2O3 solid acid catalysis |
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