CN104327135A - Extraction method of hesperidin - Google Patents

Extraction method of hesperidin Download PDF

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Publication number
CN104327135A
CN104327135A CN201310623459.2A CN201310623459A CN104327135A CN 104327135 A CN104327135 A CN 104327135A CN 201310623459 A CN201310623459 A CN 201310623459A CN 104327135 A CN104327135 A CN 104327135A
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Prior art keywords
orange peel
hesperidin
supersound extraction
extract
obtains
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CN201310623459.2A
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Chinese (zh)
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钟延华
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Individual
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Priority to CN201310623459.2A priority Critical patent/CN104327135A/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
    • C07H17/06—Benzopyran radicals
    • C07H17/065—Benzo[b]pyrans
    • C07H17/07—Benzo[b]pyran-4-ones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses an extraction method of hesperidin, and belongs to the technical field of plant effective ingredients. According to the extraction method, citrus peels that are discarded as wastes by people in the past are used as raw materials; and hesperidin is extracted from the citrus peels by using technologies such as ultrasonic extraction, concentration, crystallization and resin adsorption so as to obtain a hesperidin product. The hesperidin product can be sold. The citrus peels are fully utilized, and economic benefits of enterprises are increased.

Description

The extracting method of Hesperidin
Technical field
The present invention relates to effective ingredients in plant extractive technique field, especially a kind of method extracting Hesperidin from orange peel.
Background technology
The oranges and tangerines resource of China is very abundant, but is also only confined to outside the utilization to citrusfruit to the utilization of oranges and tangerines at present, other fail to be fully used as orange peel.Orange peel accounts for 20% of whole fruit weight, and common treatment process is discarded, and this causes very large waste, also causes pollution to environment simultaneously.Except containing except moisture, Mierocrystalline cellulose, xylogen in orange peel, orange peel is also containing abundant pigment, pectin, Hesperidin, essence wet goods.Therefore, one of the main path that utilizes of orange peel around its ingredient extraction and launch, as therefrom extracted pigment, pectin, Hesperidin, volatile oil, food fibre etc.
Wherein Hesperidin is the one of flavonoid compound, discovered in recent years its have hypotensive, antianaphylaxis, the bone density that reduces, cholesterol, enzymic activity in change body, improve microcirculation, antibacterial, anti-inflammatory, anti-hepatitis, the pharmacological action such as anticancer, it is the medicine for the treatment of hypertension and myocardial infarction, being used as the raw material of pharmacy in medicine industry, is one of main composition of Chinese patent medicine Maitong.
Summary of the invention
The object of this invention is to provide a kind of extracting method of Hesperidin, the method can fully utilize originally be used as the orange peel that refuse abandons.
The technical scheme of technical solution problem of the present invention is:
An extracting method for Hesperidin, comprises the following steps:
A, select fresh oranges and tangerines, gather its orange peel and carry out drying, and pulverized 50 order ~ 60 mesh sieves and obtain orange peel powder;
B. ultrasonic extraction device put into by orange peel powder steps A obtained and long-pending 95% ethanol of 5 ~ 10 times of orange peel powders, mix, carry out supersound extraction 2 ~ 3 times, the extracting liquid filtering each supersound extraction obtained also merging obtains orange peel extracting solution; Wherein, each supersound extraction ultrasonic power be 70W/cm 2~ 80 W/cm 2, each supersound extraction frequency is 40 KHz ~ 60 KHz, and each supersound extraction temperature is 40 DEG C ~ 55 DEG C, and each supersound extraction time is 30min ~ 90min;
C. it is 1.0 ~ 1.2 that the orange peel extracting solution obtained by step B carries out being evaporated to relative density, obtains orange peel and extracts cream, reclaim ethanol simultaneously; Regulate orange peel to extract cream PH to 3.5 ~ 4.5 with hydrochloric acid soln, leave standstill crystallization 12 ~ 24 hours, then filter and obtain thick Hesperidin extract;
D. the thick Hesperidin extract obtained by step C is dissolved in distilled water, and stirs, and upper macroporous adsorptive resins, then uses 75% ethanol elution, collects elutriant drying obtains Hesperidin.
Owing to adopting technique scheme, the beneficial effect that the present invention obtains is:
The present invention adopts and was used as orange peel that refuse abandons as starting material by people in the past, utilize supersound extraction, concentrated, crystallization, resin absorption Technology Hesperidin is carried out to orange peel extract and obtain Hesperidin product; Product Hesperidin can bring sale, so the invention enables orange peel to be fully used, and the economic profit of the enterprise of also improving.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1
The extracting method of Hesperidin comprises the following steps:
A, select fresh oranges and tangerines 1 ton, gather its orange peel and carry out drying, and pulverized 50 mesh sieves and obtain orange peel powder;
B. ultrasonic extraction device put into by orange peel powder steps A obtained and long-pending 95% ethanol of 5 times of orange peel powders, mixes, carries out supersound extraction 2 times, and the extracting liquid filtering each supersound extraction obtained also merges and obtains orange peel extracting solution; Wherein, each supersound extraction ultrasonic power be 80 W/cm 2, each supersound extraction frequency is 60 KHz, and each supersound extraction temperature is 55 DEG C, and each supersound extraction time is 90min;
C. it is 1.0 that the orange peel extracting solution obtained by step B carries out being evaporated to relative density, obtains orange peel and extracts cream, reclaim ethanol simultaneously; Regulate orange peel to extract cream PH to 3.5 with hydrochloric acid soln, leave standstill crystallization 12 hours, then filter and obtain thick Hesperidin extract;
D. the thick Hesperidin extract obtained by step C is dissolved in distilled water, and stirs, and upper macroporous adsorptive resins, then uses 75% ethanol elution, collects elutriant drying obtains Hesperidin.
Embodiment 2
The extracting method of Hesperidin comprises the following steps:
A, select fresh oranges and tangerines 50 kilograms, gather its orange peel and carry out drying, and pulverized 55 mesh sieves and obtain orange peel powder;
B. ultrasonic extraction device put into by orange peel powder steps A obtained and long-pending 95% ethanol of 8 times of orange peel powders, mixes, carries out supersound extraction 3 times, and the extracting liquid filtering each supersound extraction obtained also merges and obtains orange peel extracting solution; Wherein, each supersound extraction ultrasonic power be 75 W/cm 2, each supersound extraction frequency is 50 KHz, and each supersound extraction temperature is 50 DEG C, and each supersound extraction time is 60min;
C. it is 1.1 that the orange peel extracting solution obtained by step B carries out being evaporated to relative density, obtains orange peel and extracts cream, reclaim ethanol simultaneously; Regulate orange peel to extract cream PH to 4.0 with hydrochloric acid soln, leave standstill crystallization 18 hours, then filter and obtain thick Hesperidin extract;
D. the thick Hesperidin extract obtained by step C is dissolved in distilled water, and stirs, and upper macroporous adsorptive resins, then uses 75% ethanol elution, collects elutriant drying obtains Hesperidin.
Embodiment 3
The extracting method of Hesperidin comprises the following steps:
A, select fresh oranges and tangerines 10 kilograms, gather its orange peel and carry out drying, and pulverized 60 mesh sieves and obtain orange peel powder;
B. ultrasonic extraction device put into by orange peel powder steps A obtained and long-pending 95% ethanol of 10 times of orange peel powders, mixes, carries out supersound extraction 3 times, and the extracting liquid filtering each supersound extraction obtained also merges and obtains orange peel extracting solution; Wherein, each supersound extraction ultrasonic power be 70W/cm 2, each supersound extraction frequency is 40 KHz, and each supersound extraction temperature is 40 DEG C, and each supersound extraction time is 30min;
C. it is 1.2 that the orange peel extracting solution obtained by step B carries out being evaporated to relative density, obtains orange peel and extracts cream, reclaim ethanol simultaneously; Regulate orange peel to extract cream PH to 4.5 with hydrochloric acid soln, leave standstill crystallization 24 hours, then filter and obtain thick Hesperidin extract;
D. the thick Hesperidin extract obtained by step C is dissolved in distilled water, and stirs, and upper macroporous adsorptive resins, then uses 75% ethanol elution, collects elutriant drying obtains Hesperidin.

Claims (1)

1. an extracting method for Hesperidin, is characterized in that comprising the following steps:
A, select fresh oranges and tangerines, gather its orange peel and carry out drying, and pulverized 50 order ~ 60 mesh sieves and obtain orange peel powder;
B. ultrasonic extraction device put into by orange peel powder steps A obtained and long-pending 95% ethanol of 5 ~ 10 times of orange peel powders, mix, carry out supersound extraction 2 ~ 3 times, the extracting liquid filtering each supersound extraction obtained also merging obtains orange peel extracting solution; Wherein, each supersound extraction ultrasonic power be 70W/cm 2~ 80 W/cm 2, each supersound extraction frequency is 40 KHz ~ 60 KHz, and each supersound extraction temperature is 40 DEG C ~ 55 DEG C, and each supersound extraction time is 30min ~ 90min;
C. it is 1.0 ~ 1.2 that the orange peel extracting solution obtained by step B carries out being evaporated to relative density, obtains orange peel and extracts cream, reclaim ethanol simultaneously; Regulate orange peel to extract cream PH to 3.5 ~ 4.5 with hydrochloric acid soln, leave standstill crystallization 12 ~ 24 hours, then filter and obtain thick Hesperidin extract;
D. the thick Hesperidin extract obtained by step C is dissolved in distilled water, and stirs, and upper macroporous adsorptive resins, then uses 75% ethanol elution, collects elutriant drying obtains Hesperidin.
CN201310623459.2A 2013-12-01 2013-12-01 Extraction method of hesperidin Pending CN104327135A (en)

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Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
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CN104327135A true CN104327135A (en) 2015-02-04

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107495111A (en) * 2017-09-08 2017-12-22 广东日可威食品原料有限公司 A kind of health care fermented rice cake for preventing and treating high fat of blood and preparation method thereof
CN112088839A (en) * 2020-10-21 2020-12-18 江西天韵农业开发股份有限公司 Feeding method for improving meat quality and flavor of cage-cultured laying ducks

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2348215A (en) * 1940-08-03 1944-05-09 Fruit Growers Exchange Ca Process for the manufacture of hesperidin
CN101643489A (en) * 2009-05-26 2010-02-10 苏州派腾生物医药科技有限公司 Process for preparing hesperidin
KR101119311B1 (en) * 2009-12-02 2012-03-16 한국식품연구원 Purification method of hesperidin from mandarin peel
CN102442916A (en) * 2010-10-01 2012-05-09 华中农业大学 Method for extracting natural activated products from orange drops
CN102617673A (en) * 2012-02-25 2012-08-01 浙江大学 Method for separating and purifying naringin and neohesperidin from white skin layer of citrus grandis
CN102839053A (en) * 2012-08-23 2012-12-26 高伟 Method for extracting aurantiamarin from orange peel

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2348215A (en) * 1940-08-03 1944-05-09 Fruit Growers Exchange Ca Process for the manufacture of hesperidin
CN101643489A (en) * 2009-05-26 2010-02-10 苏州派腾生物医药科技有限公司 Process for preparing hesperidin
KR101119311B1 (en) * 2009-12-02 2012-03-16 한국식품연구원 Purification method of hesperidin from mandarin peel
CN102442916A (en) * 2010-10-01 2012-05-09 华中农业大学 Method for extracting natural activated products from orange drops
CN102617673A (en) * 2012-02-25 2012-08-01 浙江大学 Method for separating and purifying naringin and neohesperidin from white skin layer of citrus grandis
CN102839053A (en) * 2012-08-23 2012-12-26 高伟 Method for extracting aurantiamarin from orange peel

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
YAQIN MA ET AL.: "Ultrasound-assisted extraction of hesperidin from penggan(Citrus reticulata) peel", 《ULTRASONICS SONOCHEMISTRY》, vol. 15, 2 April 2007 (2007-04-02), pages 227 *
李荣等: "橙皮苷的提取及其抗炎活性研究", 《安徽医科大学学报》, vol. 42, no. 5, 31 October 2007 (2007-10-31) *
杨佳等: "大孔树脂分离纯化脐橙皮中橙皮苷的工艺研究", 《食品科技》, vol. 35, no. 6, 20 June 2010 (2010-06-20), pages 213 *
贾亚伟等: "橙皮苷提取工艺研究进展", 《中国科技论文在线》, vol. 6, no. 3, 31 March 2011 (2011-03-31) *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107495111A (en) * 2017-09-08 2017-12-22 广东日可威食品原料有限公司 A kind of health care fermented rice cake for preventing and treating high fat of blood and preparation method thereof
CN112088839A (en) * 2020-10-21 2020-12-18 江西天韵农业开发股份有限公司 Feeding method for improving meat quality and flavor of cage-cultured laying ducks

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Application publication date: 20150204