CN107997159A - A kind of microencapsulation seeweed polyphenol extract and preparation method thereof - Google Patents
A kind of microencapsulation seeweed polyphenol extract and preparation method thereof Download PDFInfo
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- CN107997159A CN107997159A CN201711421254.0A CN201711421254A CN107997159A CN 107997159 A CN107997159 A CN 107997159A CN 201711421254 A CN201711421254 A CN 201711421254A CN 107997159 A CN107997159 A CN 107997159A
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- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 108
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 107
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 238000000605 extraction Methods 0.000 title claims description 18
- 239000000463 material Substances 0.000 claims abstract description 22
- 150000004676 glycans Chemical class 0.000 claims abstract description 13
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 13
- 239000005017 polysaccharide Substances 0.000 claims abstract description 13
- 238000010521 absorption reaction Methods 0.000 claims abstract description 7
- 239000003094 microcapsule Substances 0.000 claims abstract description 7
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 241000199919 Phaeophyceae Species 0.000 claims abstract description 5
- 229920001661 Chitosan Polymers 0.000 claims abstract description 4
- 229920002774 Maltodextrin Polymers 0.000 claims abstract description 4
- 239000005913 Maltodextrin Substances 0.000 claims abstract description 4
- 229940035034 maltodextrin Drugs 0.000 claims abstract description 4
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 3
- 229920002472 Starch Polymers 0.000 claims abstract description 3
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000008107 starch Substances 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 34
- 241001474374 Blennius Species 0.000 claims description 11
- 239000003480 eluent Substances 0.000 claims description 10
- 239000011347 resin Substances 0.000 claims description 9
- 229920005989 resin Polymers 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000010828 elution Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 6
- 239000003292 glue Substances 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 4
- 229910001410 inorganic ion Inorganic materials 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 241000195474 Sargassum Species 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 241001261505 Undaria Species 0.000 claims 1
- 238000004064 recycling Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 14
- 238000005516 engineering process Methods 0.000 description 6
- 235000019658 bitter taste Nutrition 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000011149 active material Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- 244000294611 Punica granatum Species 0.000 description 1
- 235000014360 Punica granatum Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241001261506 Undaria pinnatifida Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- QCDYQQDYXPDABM-UHFFFAOYSA-N phloroglucinol Chemical compound OC1=CC(O)=CC(O)=C1 QCDYQQDYXPDABM-UHFFFAOYSA-N 0.000 description 1
- 229960001553 phloroglucinol Drugs 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- -1 polyphenol compound Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to the technical field of seeweed polyphenol, a kind of microencapsulation brown algae polyphenols extract, core is seeweed polyphenol extract, and wall material is that mass ratio is 1:35 Arabic gums and polysaccharide, wherein polysaccharide are porous-starch, one or more kinds of equal proportion mixtures in chitosan, maltodextrin, cyclodextrin.The present invention seeweed polyphenol extract separation method and microcapsule preparation process it is reasonable, be effectively combined, technological process is simplified on the premise of any equipment is not increased, has saved production cost, at the same time, stability, physical behavior and the mouthfeel of microencapsulation seeweed polyphenol extract are improved, and solve the problems such as unstable oxidizable rotten and easy moisture absorption of brown algae polyphenols extract and bad mouthfeel to a certain extent.
Description
Technical field
The present invention relates to the technical field of seeweed polyphenol, and in particular to the preparation method of seeweed polyphenol extract.
Background technology
Seeweed polyphenol (algae polyphenols, AP) is the polyphenol compound general name extracted from seaweed, is
Has the secondary metabolite of seaweed, and seaweed protects itself from the active principle of adventive interference.Seeweed polyphenol conduct
The main source of sea-plant polyphenol, the land plant polyphenol such as its structure and tea polyphenols, apple polyphenol, Punica granatum L. polyphenol exist obvious
Difference, seeweed polyphenol basic structural unit are phloroglucins, and simply phloroglucin connection mode is different with quantity.Wherein in brown alga
Polyphenol content is compared with horn of plenty, and brown alga has the characteristics that distribution is wide, species is more, yield is high, so brown alga is that extraction separation is more
The excellent algae of phenol.
Seeweed polyphenol have it is antitumor, anti-oxidant, numerous physiological activity such as antibacterial, antiviral, not only there is very strong pharmacology
Activity, and brown algae polyphenols belong to less toxic chemical substance, edible safety.In October, 2017 major website orientation European Union assessment kelp
News of the brown algae polyphenols extract as the security of new resource food, it is food supplement to claim this new resource food work done in the manner of a certain author
For the normal adults of more than 12 years old, with people's multi-functional understanding many to polyphenol and the further depth of plant polyphenol research
Enter, can predict that seeweed polyphenol future will have wider array of market development prospect.
The research of current seeweed polyphenol is concentrated mainly on the preparation of the extracting method and high-purity polyphenol product of polyphenol
In improvement, such as Chinese patent CN201510529461.2;CN201710070691.6;CN2012105226792.7;
CN201610611275.8;CN201510648035.0, but these patents be only absorbed in the extraction process of polyphenol, extraction efficiency and
Product purity, and the unstability of polyphenol property is ignored, polyphenol is easily aoxidized, particularly easily rotten under light illumination, at the same time
Polyphenol contains multiple hydroxyls, easily forms hydrogen bond with water so that and, there is the characteristics of not easy to maintain in dried sample easy to absorb moisture,
And some techniques are complex or equipment requirement is higher, be not suitable for large-scale production.Meanwhile seeweed polyphenol is as exclusive
Sea-plant property polyphenol, its extract influence eating mouth feel, always there are the fishy smell of a degree of seaweed and the bitter taste of polyphenol
It, due to presence of the seeweed polyphenol there are content the problems such as relatively low, unstable oxidizable, mouthfeel is bad, limits seeweed polyphenol
Industrialized development, therefore there is an urgent need to develop the product that a kind of polyphenol content is high, property is stable, is easy to preserve and eats.
Microencapsulation technology is a kind of technology for using different wall materials to be embedded active material, and wall material has natural polymer
Sub- material and synthesis high molecular material, and apply the features such as natural macromolecular material is because of stabilization, nontoxic, good film-forming property it is more,
More commonly used natural macromolecular material has glue, polysaccharide, protein etc.., can by selecting different wall materials to carry out embedding treatment
Playing improves the stability of active material, changes its physical property, control release, isolation active ingredient, shielding taste and smell
Etc. a series of effects..
The content of the invention
It is an object of the invention to solve bad current seeweed polyphenol stability, the easy moisture absorption, mobility is bad and mouthfeel is owed
Good problem, there is provided a kind of microencapsulation seeweed polyphenol extract, extraction and separation process are combined with microencapsulation technology of preparing, are made micro-
Encapsulated seeweed polyphenol extract stability is good, being not easy the moisture absorption, mobility, good, mouthfeel is easy to receive.In addition the present invention also provides one
The preparation method of kind microencapsulation seeweed polyphenol extract, technique is simple, easily operated, and it is excellent that properties of product are made.
For achieving the above object, the present invention uses following technical scheme:
A kind of microencapsulation seeweed polyphenol extract, core are seeweed polyphenol extract, and wall material is that mass ratio is 1:3-5 Arabic gums
And polysaccharide, wherein polysaccharide is one or more kinds of equal proportion mixtures in chitosan, maltodextrin, cyclodextrin.
The preparation method of microencapsulation seeweed polyphenol extract of the present invention, comprises the following steps:
The extraction of step (1), seeweed polyphenol:New fresh seaweed is sieved after drying and crushing, is added 50-90% ethanol and is extracted,
Wherein solid-liquid ratio (m/v) is 1:15-25, Extracting temperature are 30-50 DEG C, when extraction time is 4-6 small;
The separation of step (2), seeweed polyphenol:By step(One)The extracting solution of acquisition is concentrated under reduced pressure at 40-60 DEG C recycles ethanol,
The macroreticular resin handled well on concentrate, is first eluted with pure water after the completion of absorption and removes polysaccharide, inorganic ions impurity, then use body
Product concentration 50-90% ethanol elutions obtain polyphenol eluent, elution speed 2-4BV/h, and eluent dosage is 3-5BV, and polyphenol elutes
Liquid is recovered under reduced pressure ethanol and obtains seeweed polyphenol extracting solution, and polyphenol content is more than 30% in this extracting solution;
The preparation of step (3), seeweed polyphenol microcapsules:By step(Two)In seeweed polyphenol extracting solution as core, with me
Primary glue and polysaccharide are wall material, and first wall material is dissolved in pure water, are made into the solution that concentration is 1-5%, then add seeweed polyphenol and carry
Take in liquid and stir evenly, it is 8%-12% to be concentrated under reduced pressure into solid content, and spray drying obtains microencapsulation seeweed polyphenol extract.
Wherein, the step(One)The high brown alga of middle seaweed, preferably polyphenol content, including kelp, kelp, sargassum fusifome, skirt
Band dish and its sporophyll etc..
The step(Two)Middle macroreticular resin is AB-8 or NKA-9 type macroreticular resins
The step(Three)Middle wall material and core mass ratio 1-3:1.
Using above-mentioned technique it is a technical advantage that the following aspects:
(1)By taking a variety of methods, the higher seeweed polyphenol extract of content is obtained(Polyphenol content is more than 30%), such as choose
The high brown alga of polyphenol content is extracted for raw material, using the ethanol of various concentrations, using macroreticular resin further clean and pure
Change.
(2)By the extraction process of seeweed polyphenol with microcapsule preparation process rationally, be effectively combined, realize once
Property feed intake and can obtain the product of microencapsulation, technological process is simple, rationally, on the premise of equipment is not increased, shortens life
The flow of production. art and time, and it is easy to the production of large-scale.
(3)Pass through the technology of preparing of microcapsules so that stability, physical behavior and the mouthfeel of seeweed polyphenol product obtain
Improve, the fishy smell of seaweed and the bitter taste of polyphenol decrease in the product produced, while product has that stability is good, flowing
Property it is good and the characteristics of be not easy the moisture absorption, be conducive to holding and use and the increase bioavilability of product, so that it is more to expand seaweed
Application of the phenol in each field.
Embodiment
The preparation process of microencapsulation seeweed polyphenol extract of the present invention is made by specific examples below mode more detailed
Explanation:
Embodiment one:
Microencapsulation seeweed polyphenol extract, prepares according to following step:
The extraction of step (1), seeweed polyphenol:By dry Undaria pinnatifida Suringar 500g grinding and sievings, it is 50% to add concentration
Ethanol is extracted, and solid-liquid ratio (m/v) is 1:20,50 DEG C of Extracting temperature, when extraction time is 6 small;
The separation of step (2), seeweed polyphenol:By step(One)The extracting solution of acquisition is concentrated under reduced pressure at 60 DEG C recycles ethanol to one
Determine volume, the AB-8 macroreticular resins handled well on concentrate, are first eluted with pure water after completion of the sample, remove polysaccharide, inorganic ions
Deng impurity, polyphenol eluent then is obtained with 90% ethanol elution, elution speed is 3 BV/h, and eluent dosage is 3BV, and polyphenol is washed
De- liquid is recovered under reduced pressure ethanol and obtains seeweed polyphenol extracting solution, and polyphenol content is 51.2% in this extracting solution;
The preparation of step (3), seeweed polyphenol microcapsules:By step(Two)In seeweed polyphenol extracting solution as core, with me
Primary glue, chitosan are wall material(Both mass ratioes are 1:1), first by wall material be made into certain volume pure water concentration be 3% it is molten
Liquid, by wall material and core 3:1 ratio is added in seeweed polyphenol extracting solution and stirred evenly, and being concentrated under reduced pressure into solid content is
It is spray-dried after 10% certain volume, it is final to obtain microencapsulation seeweed polyphenol extract.
Embodiment two:
Microencapsulation seeweed polyphenol extract, prepares according to following step:
The extraction of step (1), seeweed polyphenol:By dry skirt kelp 500g grinding and sievings, add concentration for 90% ethanol into
Row extraction, solid-liquid ratio 1:15,30 DEG C of Extracting temperature, when extraction time is 4 small;
The separation of step (2), seeweed polyphenol:By step(One)The extracting solution of acquisition is concentrated under reduced pressure at 40 DEG C recycles ethanol to one
Determine volume, the NKA-9 macroreticular resins handled well on concentrate, are first eluted after loading is complete with pure water, remove polysaccharide, inorganic ions etc.
Impurity, then obtains polyphenol eluent, elution speed 2BV/h with 70% ethanol elution, and eluent dosage is 4BV, polyphenol eluent
Ethanol is recovered under reduced pressure and obtains seeweed polyphenol extracting solution, polyphenol content is 37.8% in this extracting solution;
The preparation of step (3), seeweed polyphenol microcapsules:By step(Two)In seeweed polyphenol extracting solution as core, with me
Primary glue, porous-starch, maltodextrin are wall material(Both mass ratioes are 1:2:2), first it is made into through wall material with certain volume pure water dense
The solution for 5% is spent, by wall material and core 1:1 ratio is added in seeweed polyphenol extracting solution and stirred evenly, and is concentrated under reduced pressure into solid
Thing content is final to obtain microencapsulation seeweed polyphenol extract to be spray-dried after 12% certain volume.
Measure product refrigerate and room temperature preservation under the conditions of, the change of polyphenol content, the results show that with polyphenol extract phase
Comparing, the seeweed polyphenol extract stability of microencapsulation substantially increases, and is increased to minimum 3 months from the storage cycle of one month,
The physical behavior of product and mouthfeel are improved at the same time, and the fishy smell of seaweed and the bitter taste of polyphenol decrease, and product
Powder is uniform, and good fluidity, is not easy adhesion, is not easy the moisture absorption, is conducive to the holding and use of product.
The above is merely illustrative of the technical solution of the present invention, every technology based on claims of the present invention content
Scheme, belongs to protection scope of the present invention.
Claims (5)
1. a kind of microencapsulation seeweed polyphenol extract, it is characterised in that core is seeweed polyphenol extract, and wall material is that mass ratio is
1:3-5 Arabic gums and polysaccharide, wherein polysaccharide are porous-starch, one or more etc. in chitosan, maltodextrin, cyclodextrin
Scalemic thereof.
2. the preparation method of microencapsulation brown algae polyphenols extract as described in claim 1, it is characterised in that comprise the following steps:
The extraction of step (1), seeweed polyphenol:New fresh seaweed is sieved after drying and crushing, is added 50-90% ethanol and is extracted,
Wherein solid-liquid ratio (m/v) is 1:15-25, Extracting temperature are 30-50 DEG C, when extraction time is 4-6 small;
The separation of step (2), seeweed polyphenol:By step(One)The extracting solution of acquisition is concentrated under reduced pressure at 40-60 DEG C recycles ethanol,
The macroreticular resin handled well on concentrate, is first eluted with pure water after the completion of absorption and removes the impurity such as polysaccharide, inorganic ions, Ran Houyong
50-90% ethanol elutions obtain polyphenol eluent, elution speed 2-4BV/h, and eluent dosage is 3-5BV, and polyphenol eluent depressurizes
Recycling ethanol obtains seeweed polyphenol extracting solution, and polyphenol content is more than 30% in this extracting solution;
The preparation of step (3), seeweed polyphenol microcapsules:By step(Two)In seeweed polyphenol extracting solution as core, with me
Primary glue and polysaccharide are wall material, and first wall material is dissolved in pure water, are made into the solution that concentration is 1-5%, then add seeweed polyphenol
Stirred evenly in extracting solution, it is 8-12% to be concentrated under reduced pressure into solid content, and spray drying obtains the extraction of microencapsulation seeweed polyphenol
Thing.
3. the preparation method of microencapsulation seeweed polyphenol extract as claimed in claim 2, it is characterised in that
The step(One)Middle seaweed, chooses the high brown alga of polyphenol content, including kelp, kelp, sargassum fusifome, undaria pinnitafida and its spore
Cotyledon.
4. the preparation method of microencapsulation seeweed polyphenol extract as claimed in claim 2, it is characterised in that the step(Two)
Middle macroreticular resin is AB-8 or NKA-9 type macroreticular resins.
5. the preparation method of microencapsulation seeweed polyphenol extract as claimed in claim 2, it is characterised in that the step(Three)
Middle wall material and core mass ratio 1-3:1 .
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CN109619315A (en) * | 2018-12-04 | 2019-04-16 | 安徽农业大学 | A kind of feed addictive and its preparation method and application improving birds, beasts and eggs polyphenol content |
CN109619315B (en) * | 2018-12-04 | 2022-11-18 | 安徽农业大学 | Feed additive for increasing polyphenol content of poultry eggs and preparation method and application thereof |
CN113133494A (en) * | 2020-01-19 | 2021-07-20 | 广州立达尔生物科技股份有限公司 | Mangosteen pericarp extract microcapsule feed additive and preparation method thereof |
CN115068623A (en) * | 2022-06-20 | 2022-09-20 | 温州大学 | Preparation method of sargassum fusiforme polysaccharide-polyphenol compound capable of stabilizing sargassum fusiforme polyphenol |
CN117137955A (en) * | 2023-08-24 | 2023-12-01 | 中国海洋大学 | Seaweed polyphenol and preparation method and application thereof |
CN117122059A (en) * | 2023-08-29 | 2023-11-28 | 中国海洋大学 | Preparation method and application of antiallergic and anti-inflammatory seaweed polyphenol |
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