CN103897020A - Method for extracting plukenetia volubilis seed protein - Google Patents
Method for extracting plukenetia volubilis seed protein Download PDFInfo
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- CN103897020A CN103897020A CN201410167941.4A CN201410167941A CN103897020A CN 103897020 A CN103897020 A CN 103897020A CN 201410167941 A CN201410167941 A CN 201410167941A CN 103897020 A CN103897020 A CN 103897020A
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- oil rattan
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Abstract
The invention discloses a method for extracting plukenetia volubilis seed protein. The method comprises the following steps: taking squeezed (extracted) plukenetia volubilis cake as a raw material; screening after drying and crushing; extracting the protein by adopting an alkali-solution and acid-isolation method after degreasing, wherein the technological parameters of alkali-solution and acid-isolation extraction are as follows: the ratio of solid to liquid, namely the mass-to-volume ratio (w/v) of degreased powder to dilute alkali lye is (1:1) to (1:100); the pH of the dilute alkali lye is 7.0-13.0; the extraction temperature is 20-80 DEG C, and the extraction time is 30-3000 minutes; separating out protein sediments after acid isolation, and drying to form protein powder. The method is simple in process, low in cost, and high in protein yield, and guidance is provided for research and development of related products of the plukenetia volubilis seed protein.
Description
Technical field
The invention belongs to food processing field, be specifically related to a kind of extracting method of astral oil rattan protein.
Background technology
Astral oil rattan (
plukenetia volubilislinneo), title prints and adds fruit, seal with peanut, U.S. rattan fruit, plukenetia volubilis linneo again, and seal adds language and claims Sacha Inchi, is the perennial bejuco of Euphorbiaceae (Euphorbiaceae), the former area, South America Andes that is grown in height above sea level 80 ~ 1700 m.Within 2006, introduce China,, within 2008, plant experimentally successfully if Xishuangbanna Tropical Plant Garden, Chinese Academy of Sciences and Guangdong Province are as planted experimentally on the ground such as South China Botanical Garden Chinese Academy of Sciences in Yunnan Province.In astral oil rattan kind benevolence except containing abundant polyunsaturated fatty acid, also contain rich in protein, its protein content reaches 25 ~ 30%, in its protein, comprise multiple amino acids and 8 kinds of indispensable amino acids, and the secondary metabolite such as rich in vitamin E, sterol, flavones and phenols and alkaloid, saponin(e.At present, be mainly the exploitation of grease for the research of astral oil rattan both at home and abroad, astral oil rattan oil has maintenance skin, delays senility, regulates the effects such as blood fat; And it is very few to the research of albumen in kind of benevolence.Astral oil rattan is as the traditional oil tree of firm successful introduction, and Ministry of Health's approved in 2013 is new food raw material, and the feature of its high protein content and amino acid equilibrium makes it aspect food, feed and agricultural byproducts processing, have very large exploitation value.
Summary of the invention
For still belonging to blank for astral oil rattan protein extraction process both at home and abroad, the invention provides a kind of technique simple, protein extracting method with low cost, productive rate is higher.
Step of the present invention.
Using the grouts after astral oil rattan kind benevolence squeezing (extractions) as raw material, is pulverized, use normal hexane (sherwood oil or other degreasing solvent) degreasing 0.5 ~ 50 h, volatilizing must spent meal after the solvent such as (or heating, air-dry).
From spent meal, extract albumen by alkali extraction and acid precipitation method, processing parameter is: solid-liquid ratio, and the mass volume ratio of spent meal and sig water (w/v) is 1:1 ~ 1:100; The pH of sig water is 7 ~ 13; Extract 20 ~ 80 DEG C of temperature; Extraction times 10 ~ 3000 min.
Repeat to extract, repeat said extracted step, the supernatant liquor of united extraction, is adjusted to iso-electric point with diluted acid, makes protein precipitation.After standing separation, wash precipitation with water, final drying, obtains astral oil rattan protein.Weigh and calculate extraction rate of protein.Extraction rate of protein is calculated as follows:
Extraction rate of protein (%)=(protein quality in alkali process gained protein quality/spent meal) × 100%.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described, and following examples are in order to set forth better the present invention, instead of are used for limiting the scope of the invention.All pick up from Xishuangbanna Tropical Plant Garden, Chinese Academy of Sciences for raw material on probation, agents useful for same is analytical pure, chemical pure, technical pure or food grade.
Embodiment 1.
Astral oil rattan grouts are pulverized, crossed 100 mesh sieves, with normal hexane degreasing 4 h, then in stink cupboard, place 12 h, volatilize after solvent to obtain spent meal.
Take spent meal 5 g, add sodium hydroxide solution (pH 12) 150 ml, stir 120 min at 50 DEG C of lower magnetic forces, then with centrifugal 20 min of speed of 4000 r/min, precipitation repeats to extract once, merges the supernatant liquor of 2 extractions.Above-mentioned supernatant liquor pH is adjusted to 3.5 with diluted acid, sedimentation 10 h at 4 DEG C, distilled water wash 3 times for the precipitation of filtering separation, finally dry albumen 2.478 g that to obtain in freeze drier, extraction yield is 76.47%.
Embodiment 2.
Astral oil rattan grouts are pulverized, crossed 80 mesh sieves, in extraction equipment for fat, with normal hexane degreasing 4 h, then in stink cupboard, place 12 h, volatilize after solvent to obtain spent meal.
Take spent meal 5 g, add sodium hydroxide solution (pH 9) 150 ml, stir 120 min at 50 DEG C of lower magnetic forces, then with centrifugal 20 min of speed of 4000 r/min, precipitation repeats to extract once, merges the supernatant liquor of 2 extractions.Above-mentioned supernatant liquor pH is adjusted to 5 with dilute hydrochloric acid, sedimentation 10 h at 4 DEG C, distilled water wash 3 times for the precipitation of filtering separation, finally dry albumen 1.812 g that to obtain in freeze drier, extraction yield is 55.92%.
Embodiment 3.
Astral oil rattan grouts are pulverized, crossed 60 mesh sieves, in extraction equipment for fat, with normal hexane degreasing 4 h, then in stink cupboard, place 12 h, volatilize after solvent to obtain spent meal.
Take spent meal 5 g, add sodium hydroxide solution (pH 12) 150 ml, stir 120 min at 60 DEG C of lower magnetic forces, then with centrifugal 20 min of speed of 4000 r/min, precipitation repeats to extract once, merges the supernatant liquor of 2 extractions.Above-mentioned supernatant liquor pH is adjusted to 4 with dilute hydrochloric acid acid, sedimentation 10 h at 4 DEG C, distilled water wash 3 times for the precipitation of filtering separation, finally dry albumen 2.201 g that to obtain in freeze drier, extraction yield is 67.92%.
Embodiment 4.
Astral oil rattan grouts are pulverized, crossed 100 mesh sieves, in extraction equipment for fat, with normal hexane degreasing 4 h, then in stink cupboard, place 12 h, volatilize after solvent to obtain spent meal.
Take spent meal 5 g, add sodium hydroxide solution (pH 12.5) 150 ml, stir 120 min at 45 DEG C of lower magnetic forces, then with centrifugal 20 min of speed of 4000 r/min, precipitation repeats to extract once, merges the supernatant liquor of 2 extractions.Above-mentioned supernatant liquor pH is adjusted to 3.5 with dilute sulphuric acid, sedimentation 10 h at 4 DEG C, distilled water wash 3 times for the precipitation of filtering separation, finally dry albumen 2.458 g that to obtain in freeze drier, extraction yield is 75.85%.
Embodiment 5.
Astral oil rattan grouts are pulverized, crossed 100 mesh sieves, in extraction equipment for fat, with normal hexane degreasing 4 h, then in stink cupboard, place 12 h, volatilize after solvent to obtain spent meal.
Take spent meal 5 g, add sodium hydroxide solution (pH 9.5) 150 ml, stir 150 min at 40 DEG C of lower magnetic forces, then with centrifugal 20 min of speed of 4000 r/min, precipitation repeats to extract once, merges the supernatant liquor of 2 extractions.Above-mentioned supernatant liquor pH is adjusted to 4 with dilute sulphuric acid, sedimentation 10 h at 4 DEG C, distilled water wash 3 times for the precipitation of filtering separation, finally dry albumen 1.942 g that to obtain in freeze drier, extraction yield is 59.93%.
Embodiment 6.
Astral oil rattan grouts are pulverized, crossed 100 mesh sieves, in extraction equipment for fat, with normal hexane degreasing 4 h, then in stink cupboard, place 12 h, volatilize after solvent to obtain spent meal.
Take spent meal 5 g, add sodium hydroxide solution (pH 11) 150 ml, stir 150 min at 45 DEG C of lower magnetic forces, then with centrifugal 20 min of speed of 4000 r/min, precipitation repeats to extract once, merges the supernatant liquor of 2 extractions.Above-mentioned supernatant liquor pH is adjusted to 4.5 with dilute sulphuric acid, sedimentation 10 h at 4 DEG C, distilled water wash 3 times for the precipitation of filtering separation, finally dry albumen 2.066 g that to obtain in freeze drier, extraction yield is 63.76%.
Embodiment 7.
Astral oil rattan grouts are pulverized, crossed 100 mesh sieves, in extraction equipment for fat, with normal hexane degreasing 4 h, then in stink cupboard, place 12 h, volatilize after solvent to obtain spent meal.
Take spent meal 5 g, add sodium hydroxide solution (pH 10.5) 150 ml, stir 180 min at 50 DEG C of lower magnetic forces, then with centrifugal 20 min of speed of 4000 r/min, precipitation repeats to extract once, merges the supernatant liquor of 2 extractions.Above-mentioned supernatant liquor pH is adjusted to 3.6 with dilute acetic acid, sedimentation 10 h at 4 DEG C, distilled water wash 3 times for the precipitation of filtering separation, finally dry albumen 2.023 g that to obtain in freeze drier, extraction yield is 62.43%.
Embodiment 8.
Astral oil rattan grouts are pulverized, crossed 100 mesh sieves, with normal hexane degreasing 4 h, then in stink cupboard, place 12 h, volatilize after solvent to obtain spent meal.
Take spent meal 5 g, add sodium hydroxide solution (pH 12) 200 ml, stir 220 min at 50 DEG C of lower magnetic forces, then with centrifugal 20 min of speed of 4000 r/min, precipitation repeats to extract once, merges the supernatant liquor of 2 extractions.Above-mentioned supernatant liquor pH is adjusted to 3.8 with dilute acetic acid, sedimentation 10 h at 4 DEG C, distilled water wash 3 times for the precipitation of filtering separation, finally dry albumen 2.336 g that to obtain in freeze drier, extraction yield is 72.09%.
Claims (6)
1. an extraction process for astral oil rattan protein, is characterized in that, comprises the following steps:
(1) raw materials pretreatment: the astral oil rattan kind benevolence grouts through squeezing or after extracting are pulverized and sieved, after degreasing, volatilize solvent and obtain spent meal;
(2) alkaline solution extraction process: solid-liquid ratio, the mass volume ratio of spent meal and sig water (w/v) is 1:1 ~ 1:100; The pH of sig water is 7.0 ~ 13.0; Extract 30 ~ 80 DEG C of temperature; Extraction times 30 ~ 3000 min, lixiviate finishes rear centrifugal, filters, and residue is repeated to extract 1 time to 10 times merging filtrate;
(3) acid is heavy: near iso-electric point, make protein precipitation with diluted acid adjusting filtrate pH, standing separation under low temperature, with distilled water (or pure water) washing precipitation;
(4) precipitation is dried, obtains astral oil rattan protein powder.
2. according to the extraction process of a kind of astral oil rattan protein claim 1 (1) Suo Shu, it is characterized in that, astral oil rattan grouts used are dry, rear 20 ~ 200 mesh sieves of crossing of pulverizing.
3. according to the extraction process of a kind of astral oil rattan protein claim 1 (2) Suo Shu, it is characterized in that, described sig water is the dilute solution of sodium hydroxide, sodium carbonate, sodium bicarbonate or other alkali.
4. according to the extraction process of a kind of astral oil rattan protein claim 1 (3) Suo Shu, it is characterized in that, described diluted acid is that dilute hydrochloric acid, dilute sulphuric acid organic acid are as formic acid, acetic acid or other acid.
5. according to the extraction process of a kind of astral oil rattan protein claim 1 (3) Suo Shu, it is characterized in that, in the heavy technique of described acid, each condition is: 0 ~ 30 DEG C of iso-electric point environs 3.0 ~ 6.0, standing separation temperature.
6. according to claim 1(4) extraction process of described a kind of astral oil rattan protein, it is characterized in that described dry seasoning (comprise dry, air-dry etc.) or oven dry, lyophilize, the centrifugal drying etc. of referring to.
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| CN201410167941.4A CN103897020A (en) | 2014-04-25 | 2014-04-25 | Method for extracting plukenetia volubilis seed protein |
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| CN201410167941.4A CN103897020A (en) | 2014-04-25 | 2014-04-25 | Method for extracting plukenetia volubilis seed protein |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814246A (en) * | 2015-04-03 | 2015-08-05 | 安徽新桥工贸有限责任公司 | Extraction method of sesame cake meal protein power |
| CN105284987A (en) * | 2015-09-21 | 2016-02-03 | 普洱联众生物资源开发有限公司 | Plukenetia volubilis Linneo energy bar |
| CN105707407A (en) * | 2016-03-07 | 2016-06-29 | 湖北工业大学 | Preparing method of protein concentrate of sacha inchi fruits |
| CN105707408A (en) * | 2016-03-07 | 2016-06-29 | 湖北工业大学 | Preparing method for protein isolate of sacha inchi fruits |
| CN105767452A (en) * | 2016-03-07 | 2016-07-20 | 湖北工业大学 | Manufacture method of sacha inchi polypeptide |
| CN107141334A (en) * | 2017-06-23 | 2017-09-08 | 常州大学 | Olive kernel albumen and its extracting method |
| CN108450963A (en) * | 2018-01-24 | 2018-08-28 | 佛山科学技术学院 | A kind of plukenetia volubilis linneo protein powder and preparation method thereof |
| CN115590108A (en) * | 2022-01-14 | 2023-01-13 | 河套学院（Cn） | Extraction process and application of sunflower seed protein for reducing blood fat |
-
2014
- 2014-04-25 CN CN201410167941.4A patent/CN103897020A/en active Pending
Non-Patent Citations (1)
| Title |
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| 赵旻 等: "星油藤种仁碱提蛋白和浓缩蛋白功能性质的比较研究", 《食品工业科技》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814246A (en) * | 2015-04-03 | 2015-08-05 | 安徽新桥工贸有限责任公司 | Extraction method of sesame cake meal protein power |
| CN105284987A (en) * | 2015-09-21 | 2016-02-03 | 普洱联众生物资源开发有限公司 | Plukenetia volubilis Linneo energy bar |
| CN105284987B (en) * | 2015-09-21 | 2019-08-30 | 普洱联众生物资源开发有限公司 | A kind of U.S. rattan fruit energy stick |
| CN105707407A (en) * | 2016-03-07 | 2016-06-29 | 湖北工业大学 | Preparing method of protein concentrate of sacha inchi fruits |
| CN105707408A (en) * | 2016-03-07 | 2016-06-29 | 湖北工业大学 | Preparing method for protein isolate of sacha inchi fruits |
| CN105767452A (en) * | 2016-03-07 | 2016-07-20 | 湖北工业大学 | Manufacture method of sacha inchi polypeptide |
| CN105707407B (en) * | 2016-03-07 | 2019-10-25 | 湖北工业大学 | A kind of manufacturing method printing odd fruit protein concentrate |
| CN107141334A (en) * | 2017-06-23 | 2017-09-08 | 常州大学 | Olive kernel albumen and its extracting method |
| CN108450963A (en) * | 2018-01-24 | 2018-08-28 | 佛山科学技术学院 | A kind of plukenetia volubilis linneo protein powder and preparation method thereof |
| CN115590108A (en) * | 2022-01-14 | 2023-01-13 | 河套学院（Cn） | Extraction process and application of sunflower seed protein for reducing blood fat |
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Application publication date: 20140702 |