CN107141334A - Olive kernel albumen and its extracting method - Google Patents
Olive kernel albumen and its extracting method Download PDFInfo
- Publication number
- CN107141334A CN107141334A CN201710483962.0A CN201710483962A CN107141334A CN 107141334 A CN107141334 A CN 107141334A CN 201710483962 A CN201710483962 A CN 201710483962A CN 107141334 A CN107141334 A CN 107141334A
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- CN
- China
- Prior art keywords
- olive
- kernel
- albumen
- extracting method
- olive kernel
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/06—Production of fats or fatty oils from raw materials by pressing
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention belongs to field of deep processing of farm products, more particularly to olive kernel albumen and its extracting method：After dento liva shelling, olive kernel skin is separated with kernel, olive-kernel oil is then separated using cold-press, again Residual oil is removed with food grade solvent, precipitation is depressurized after filtering, the olive kernel powder separated is dried, then add certain mass than distilled water, after high-speed homogenization, add NaOH solution and be adjusted to suitable ph, the stirring and leaching certain time under the conditions of 40 60 DEG C, leaching liquor is centrifuged, collect supernatant, obtain olive protein solution, then olive protein solution pH value is adjusted to acidity with HCl solution again, the olive albumen precipitated, centrifuge washing is to neutrality, it is dried to obtain olive kernel albumen.This method extracting method simple and fast, cost are low, and beneficial to industrialization production, the molecular weight of gained olive kernel albumen is main in 10 40ku, it is necessary to which amino acid content is high, is of high nutritive value.
Description
Technical field
The invention belongs to field of deep processing of farm products, more particularly to olive kernel albumen and its extracting method.
Background technology
The plantation history that olive has more than 2000 years in China, is mainly distributed on south of Gansu Province, Fujian, Guangdong, Guangxi and sea at present
The provinces such as south.Numerous studies show there are many materials beneficial to human body in olive, have from pulp to kernel to health
Greatly it is worth.Utilization to olive at present, is mainly used in squeezing olive oil, but the depth for dento liva, olive kernel is opened
Hair utilizes almost blank.Protein very abundant in dento liva, it is suitable with Food Legume, with higher application value.Therefore
The process technology of an economically viable red skin of olive is developed, and is used in olive industrial chain, the additional of product is improved
Value, has important economy and social effect to the income for increasing peasant.
The content of the invention
The invention provides olive kernel albumen and its extracting method, step is as follows：
(1) after dento liva shelling, benevolence skin is separated with kernel, olive-kernel oil is then separated using cold-press, then use
Food grade solvent removes Residual oil, depressurizes precipitation after filtering, the olive kernel powder separated is dried；
(2) added in the olive kernel powder that step (1) is obtained certain mass than distilled water, after high-speed homogenization, add
NaOH solution is adjusted to suitable ph, the stirring and leaching certain time under the conditions of 40-60 DEG C, collects filtrate；
(3) dilute HCl solution is added in step (2) and olive protein solution pH value is adjusted to acidity, the olive precipitated
Albumen.
(4) mixture obtained by step (3) is centrifuged, sub-cloud precipitation, and washed to neutrality, be dried to obtain olive kernel
Albumen.
The beneficial effects of the present invention are：This method extracting method simple and fast, cost are low, beneficial to industrialization production, institute
The molecular weight for obtaining olive kernel albumen is main in 10-40ku, it is necessary to which amino acid content is high, is of high nutritive value.
Brief description of the drawings
Fig. 1 be the embodiment of the present invention 1 in, the electrophoresis result of olive kernel albumen.
Fig. 2 is in the embodiment of the present invention 1, (ordinate is protein molecular weight to olive kernel molecular weight of albumen distribution results
Logarithm).
Fig. 3 be the embodiment of the present invention 1 in, the amino acid analysis result of olive kernel albumen.
Claims (7)
1. olive kernel albumen and its extracting method, it is characterised in that：The step of methods described is,
Olive kernel albumen and its extracting method：After dento liva shelling, benevolence skin is separated with kernel, then using cold-press point
Residual oil is removed from olive-kernel oil, then with food grade solvent, precipitation is depressurized after filtering, the olive kernel powder separated is done
It is dry, then add certain mass than distilled water, after high-speed homogenization, add NaOH solution and be adjusted to suitable ph, at 40-60 DEG C
Under the conditions of stirring and leaching certain time, leaching liquor is centrifuged, collect supernatant, obtain olive protein solution, Ran Houzai
Olive protein solution pH value is adjusted to acidity with HCl solution, the olive albumen precipitated is centrifuged and washed to neutrality, freezing
It is dried to obtain olive kernel albumen.This method extracting method simple and fast, cost are low, beneficial to industrialization production, gained olive kernel egg
White molecular weight is main in 10-40ku, it is necessary to which amino acid content is high, is of high nutritive value.
(1) after dento liva shelling, benevolence skin is separated with kernel, olive-kernel oil is then separated using cold-press, then use food
Level solvent removes Residual oil, depressurizes precipitation after filtering, the olive kernel powder separated is dried；
(2) added in the olive kernel powder that step (1) is obtained certain mass than distilled water, after high-speed homogenization, add NaOH molten
Liquid is adjusted to suitable ph, the stirring and leaching certain time under the conditions of 40-60 DEG C, collects filtrate；
(3) dilute HCl solution is added in step (2) and olive protein solution pH value is adjusted to acidity, the olive albumen precipitated；
(4) mixture obtained by step (3) is centrifuged, sub-cloud precipitation, and washed to neutrality, be dried to obtain olive kernel egg
In vain.
2. olive kernel albumen as claimed in claim 1 and its extracting method, it is characterised in that：In step (1), olive kernel oil removing
Using food grade solvent, and recoverable.
3. olive kernel albumen as claimed in claim 1 and its extracting method, it is characterised in that：Distillation described in step (2)
Water adding proportion is in 8-14 times of olive kernel powder weight.
4. olive kernel albumen as claimed in claim 1 and its extracting method, it is characterised in that：In step (2), NaOH is added molten
It is 8.5-9.5 that pH is arrived in liquid regulation.
5. olive kernel albumen as claimed in claim 1 and its extracting method, it is characterised in that：In step (2), stirring is extracted in
Carried out under state, extraction time is 1-2h.
6. olive kernel albumen as claimed in claim 1 and its extracting method, it is characterised in that：In step (3), dilute HCl is added
Olive protein solution pH value is adjusted to 4.0-5.5 by solution.
7. olive kernel albumen as claimed in claim 1 and its extracting method, it is characterised in that：In step (4), albumen can lead to
The modes such as vacuum drying, drying, microwave drying, vacuum freeze drying are closed to obtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710483962.0A CN107141334A (en) | 2017-06-23 | 2017-06-23 | Olive kernel albumen and its extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710483962.0A CN107141334A (en) | 2017-06-23 | 2017-06-23 | Olive kernel albumen and its extracting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107141334A true CN107141334A (en) | 2017-09-08 |
Family
ID=59781139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710483962.0A Pending CN107141334A (en) | 2017-06-23 | 2017-06-23 | Olive kernel albumen and its extracting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107141334A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112342258A (en) * | 2019-08-08 | 2021-02-09 | 陈徉 | Method for synchronously separating olive kernel oil and preparing antioxidant peptide |
| CN112552371A (en) * | 2020-12-24 | 2021-03-26 | 常州市芙丽佳生物科技有限公司 | Method for preparing olive kernel extract based on extraction and purification technology |
| CN112617198A (en) * | 2020-12-24 | 2021-04-09 | 常州市芙丽佳生物科技有限公司 | Preparation method of olive kernel extract |
| CN112641101A (en) * | 2020-12-24 | 2021-04-13 | 常州市芙丽佳生物科技有限公司 | Albumen powder containing olive kernel extract and preparation method thereof |
| CN112843776A (en) * | 2020-12-24 | 2021-05-28 | 常州市芙丽佳生物科技有限公司 | Purification process of olive kernel extract |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030232100A1 (en) * | 1998-04-08 | 2003-12-18 | Theoharides Theoharis C. | Compositions for treatment of diseases arising from secretion of mast cell biochemicals |
| CN103897020A (en) * | 2014-04-25 | 2014-07-02 | 中国科学院西双版纳热带植物园 | Method for extracting plukenetia volubilis seed protein |
| CN105925369A (en) * | 2016-06-20 | 2016-09-07 | 常州大学 | Method for cold-pressing olive-kernel oil step by step |
-
2017
- 2017-06-23 CN CN201710483962.0A patent/CN107141334A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030232100A1 (en) * | 1998-04-08 | 2003-12-18 | Theoharides Theoharis C. | Compositions for treatment of diseases arising from secretion of mast cell biochemicals |
| CN103897020A (en) * | 2014-04-25 | 2014-07-02 | 中国科学院西双版纳热带植物园 | Method for extracting plukenetia volubilis seed protein |
| CN105925369A (en) * | 2016-06-20 | 2016-09-07 | 常州大学 | Method for cold-pressing olive-kernel oil step by step |
Non-Patent Citations (1)
| Title |
|---|
| 冯笑笑等: "翅果油树种仁蛋白的提取工艺优化及其功能性质分析", 《食品科学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112342258A (en) * | 2019-08-08 | 2021-02-09 | 陈徉 | Method for synchronously separating olive kernel oil and preparing antioxidant peptide |
| CN112552371A (en) * | 2020-12-24 | 2021-03-26 | 常州市芙丽佳生物科技有限公司 | Method for preparing olive kernel extract based on extraction and purification technology |
| CN112617198A (en) * | 2020-12-24 | 2021-04-09 | 常州市芙丽佳生物科技有限公司 | Preparation method of olive kernel extract |
| CN112641101A (en) * | 2020-12-24 | 2021-04-13 | 常州市芙丽佳生物科技有限公司 | Albumen powder containing olive kernel extract and preparation method thereof |
| CN112843776A (en) * | 2020-12-24 | 2021-05-28 | 常州市芙丽佳生物科技有限公司 | Purification process of olive kernel extract |
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