CN103408337A - Nutrition formula used for promoting rapid growth of benthic nitzschia - Google Patents

Abstract

The invention discloses a nutrition formula used for promoting rapid growth of benthic nitzschia. The nutrition formula is characterized in that the nutrition formula comprises macro-elements, microelements and vitamins, wherein the macro-elements comprise nitrogen, phosphorus and silicon, the microelements comprise iron, zinc, copper, manganese, molybdenum and cobalt, and the vitamins comprise vitamin B1, vitamin B12 and vitamin H. According to growth characteristics of requirements of the benthic nitzschia to different nutritive salts, the formula can achieve an objective of promoting rapid growth of purebred benthic diatom cultures. The nutrition formula has reasonable nutrition collocation, and is capable of effectively promoting rapid division growth of the nitzschia, promoting absorption and utilization of a variety of nutrients, accelerating metabolism of nitzschia cells and improving the production quantity.

Description

Promote the dwell nutritional formula of rhombus algae Fast Growth of the end

Technical field

The present invention relates to a kind of nutritional formula, particularly a kind of dwell nutritional formula of rhombus algae Fast Growth of the end that promotes.

Background technology

Micro-algae of artificial culture is the Major Foods source in many animal paedomorphosis stages in aquatic ecosystem, and they can improve the nutritive value of animal.Marine benthic diatom is the important natural bait in stichopus japonicus, Bao Youti conversion of food habit stage, can promote adhering to and metamorphosis of young ginseng and Bao Miao.In the process of a large amount of cultivation benthic diatoms, except the basic ecological factors such as illumination, temperature, it is essential that the nutritive salt such as nitrogen, phosphorus, iron, silicon are similarly benthic diatom growth institute, its Nutrition and Metabolism is had to important effect, can promote the Fast Growth of benthic diatom, and present stage also without any a kind of method or reagent, utilize the above-mentioned characteristic of benthic diatom, it is carried out to growth promotion processing and intervention.

Summary of the invention

The present invention is in order to solve the existing above-mentioned deficiency of prior art, proposes a kind of dwell high efficiency nutritious salt formula of rhombus algae Fast Growth of benthic diatom, the particularly end that promotes.

Technical solution of the present invention is: a kind of dwell nutritional formula of rhombus algae Fast Growth of the end that promotes, it is characterized in that: described nutritional formula comprises major element, trace element and VITAMIN, wherein major element comprises nitrogen, phosphorus, silicon, trace element comprises iron, zinc, copper, manganese, molybdenum, cobalt, and VITAMIN comprises vitamins B ₁, vitamins B ₁₂, vitamin H, and the concentration range of each component is as follows:

Nitrogen: 12.36-37.08 mg/L

Phosphorus: 2.27-2.84 mg/L

Silicon: 11.50-34.51 mg/L

Iron: 2.00-4.00 mg/L

Zinc: 0.65-6.54 μ g/L

Copper: 1.91-2.54 μ g/L

Manganese: 0-49.44 μ g/L

Molybdenum: 2.88-6.72 μ g/L

Cobalt: 2.95-5.89 μ g/L

Vitamins B ₁: 100-10000 μ g/L

Vitamins B ₁₂: 0.5-25.0 μ g/L

Vitamin H: 0.5-5.0 μ g/L.

In described nutritional formula, the concentration of each component is:

Nitrogen: 37.08 mg/L

Phosphorus: 2.84 mg/L

Silicon: 23.01 mg/L

Iron: 3.33 mg/L

Zinc: 6.54 μ g/L

Copper: 2.54 μ g/L

Manganese: 49.44 μ g/L

Molybdenum: 2.88 μ g/L

Cobalt: 29.5 μ g/L

Vitamins B ₁: 100 μ g/L

Vitamins B ₁₂: 0.5 μ g/L

Vitamin H: 0.5 μ g/L.

The present invention compared with the existing technology, has following advantage:

Formula disclosed in this invention, according to dwelling the rhombus algae to the growth characteristics of Different Nutrition salt demand in the end, reach the purpose that promotes purebred benthic diatom algae kind Fast Growth.In the present invention, the nutritional formula collocation rationally, can promote the quick division growth of rhombus algae effectively, impels it to absorb better and utilizes various nutritive substances, accelerates the metabolism of rhombus frustule, improves its turnout; And the rhombus algae can be applied to as natural bait in growing seedlings of the marine economic animals such as stichopus japonicus and breeding process, strengthen its immunizing power and disease resistance, improve its nutritive value, to produce healthy green marine products economic animal seed, improve sea farming economic benefit and social benefit, the speed of growth that therefore improves the rhombus algae has larger meaning economically.Each component steady sources in the present invention's formula, with low cost, operation is simple and feasible, therefore can say that it has possessed market outlook widely, is particularly suitable for applying in aquaculture field.

The accompanying drawing explanation

Fig. 1 is embodiment of the present invention control group and the growth curve chart of the rhombus algae of different embodiment.

Embodiment

Below in conjunction with accompanying drawing explanation the specific embodiment of the present invention.

The nutritional formula embodiment 1,2,3 of rhombus algae, as shown in table 1.

Table 1 rhombus algae formulation Example

Element	Control group	Embodiment	1	Embodiment 2	Embodiment 3
						N（mg/L）	12.36	37.08	12.36	37.08
P（mg/L）	1.14	2.84	2.27	2.84
					Si（mg/L）	2.30	11.50	34.51	23.01
Fe（mg/L）	0.65	4.00	2.00	3.33
					Zn（μg/L）	6.54	0.65	0.65	6.54
Cu（μg/L）	2.54	1.91	1.91	2.54
					Mn（μg/L）	49.44	27.47	0	49.44
Mo（μg/L）	2.88	6.72	6.72	2.88
					Co（μg/L）	2.95	5.89	2.95	2.95
VB 1 （μg/L）	100	8000	10000	100
					VB 12（μg/L）	0.5	25.0	5.0	0.5
VH （μg/L）	0.5	5.0	0.5	0.5

In the seawater of boiling sterilization, add each desired substance according to the concentration of control group in table 1 and embodiment 1,2,3, and the list of inoculating therein certain density is planted the rhombus algae, after hungry 2d, cultivate, incubation time is 10 d, during every 2 d measure the OD of a benthic diatom liquid ₆₈₀, to monitor single growth tendency of planting the rhombus algae, and calculate its specific growth rate, i.e. μ=(lnOD ₂-lnOD ₁)/(t ₂-t ₁), OD ₁And OD ₂Be the optical density value at 680 nm places, t ₁And t ₂For corresponding growth number of days.

Simultaneously, inoculate three groups of lists with the same density of control group and plant the rhombus algae, and with the nutritional formula of embodiments of the invention 1,2,3, cultivate the rhombus algae respectively, after same hungry 2d, cultivate, incubation time is 10d, and every 4 d add one time of nutrition salt (namely adding nutritive salt at 4d and 8d respectively), when the 10th d, results benthic diatom algae mud, weigh, and calculates biomass.

Result of use is as follows:

In the incubation time of 10 d, the growth curve chart of single kind rhombus algae as shown in Figure 1.Control group rhombus algae namely reaches exponential growth latter stage at the 6th d, and the rhombus algae that the embodiment of the present invention 1,2,3 is cultivated still is exponential growth at the 10th d, has obviously extended its exponential phase of growth, and short long-acting fruit is obvious.With single rhombus algae growth tendency of planting that the embodiment of the present invention 1,2,3 is cultivated, all obviously be greater than control group, single rhombus algae growth tendency of planting that embodiment 3 cultivates is the most obvious, short long-acting fruit the best.

The specific growth rate of table 2 rhombus algae

Specific growth rate	μ 2	μ 4	μ 6	μ 8	μ 10
						Control group	0.316	0.269	0.226	0.182	0.144
Embodiment 1	0.290	0.309	0.237	0.187	0.164
						Embodiment 2	0.343	0.338	0.245	0.188	0.163
Embodiment 3	0.463	0.365	0.270	0.209	0.169

Annotate: μ _nThe specific growth rate of representative when incubation time is n d.

As shown in table 2, in the incubation time of 10 d, single kind rhombus algae that embodiment 1,2,3 turns out reaches maximum specific growth rate at the 6th d, simultaneously at incubation time, be the 2nd, 4,6,8, the specific growth rate during 10 d all extremely significantly is greater than control group, wherein the corresponding specific growth rate of embodiment 3 all is significantly higher than except embodiment 1,2(the 8th d), the short long-acting fruit of rhombus algae is obvious, culture effect the best.

The biomass of table 3 rhombus algae

Biomass (g/m 2）	Control group	Embodiment	1	Embodiment 2	Embodiment 3
						The rhombus algae	27.12	318.09	309.47	368.12

Biomass such as the table 3 of the rhombus algae of cultivating with embodiments of the invention 1,2,3, compare with control group, and biomass significantly improves, and is respectively 9.38,9.13,10.86 times of control group, and short long-acting fruit is extremely obvious.Wherein the biomass of embodiment 3, apparently higher than embodiment 1,2, is urged long-acting fruit the best.

Claims (2)

1. one kind promotes the dwell nutritional formula of rhombus algae Fast Growth of the end, it is characterized in that: described nutritional formula comprises major element, trace element and VITAMIN, wherein major element comprises nitrogen, phosphorus, silicon, and trace element comprises iron, zinc, copper, manganese, molybdenum, cobalt, and VITAMIN comprises vitamins B ₁, vitamins B ₁₂, vitamin H, and the concentration range of each component is as follows:

Nitrogen: 12.36-37.08 mg/L

Phosphorus: 2.27-2.84 mg/L

Silicon: 11.50-34.51 mg/L

Iron: 2.00-4.00 mg/L

Zinc: 0.65-6.54 μ g/L

Copper: 1.91-2.54 μ g/L

Manganese: 0-49.44 μ g/L

Molybdenum: 2.88-6.72 μ g/L

Cobalt: 2.95-5.89 μ g/L

Vitamins B ₁: 100-10000 μ g/L

Vitamins B ₁₂: 0.5-25.0 μ g/L

Vitamin H: 0.5-5.0 μ g/L.

2. dwell at the bottom of the promotion according to claim 1 nutritional formula of rhombus algae Fast Growth, it is characterized in that: in described nutritional formula, the concentration of each component is:

Nitrogen: 37.08 mg/L

Phosphorus: 2.84 mg/L

Silicon: 23.01 mg/L

Iron: 3.33 mg/L

Zinc: 6.54 μ g/L

Copper: 2.54 μ g/L

Manganese: 49.44 μ g/L

Molybdenum: 2.88 μ g/L

Cobalt: 29.5 μ g/L

Vitamins B ₁: 100 μ g/L

Vitamins B ₁₂: 0.5 μ g/L

Vitamin H: 0.5 μ g/L.

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