CN103408337B - Nutrition formula used for promoting rapid growth of benthic nitzschia - Google Patents

Abstract

The invention discloses a nutrition formula used for promoting rapid growth of benthic nitzschia. The nutrition formula is characterized in that the nutrition formula comprises macro-elements, microelements and vitamins, wherein the macro-elements comprise nitrogen, phosphorus and silicon, the microelements comprise iron, zinc, copper, manganese, molybdenum and cobalt, and the vitamins comprise vitamin B1, vitamin B12 and vitamin H. According to growth characteristics of requirements of the benthic nitzschia to different nutritive salts, the formula can achieve an objective of promoting rapid growth of purebred benthic diatom cultures. The nutrition formula has reasonable nutrition collocation, and is capable of effectively promoting rapid division growth of the nitzschia, promoting absorption and utilization of a variety of nutrients, accelerating metabolism of nitzschia cells and improving the production quantity.

Description

The nutritive medium promoting to dwell the end rhombus algae grows fast

Technical field

The present invention relates to a kind of nutritive medium, particularly a kind of promote the end to dwell nutritive medium that rhombus algae grows fast.

Background technology

Micro-algae of artificial culture is the main food source in many animal paedomorphosis stages in aquatic ecosystem, and they can improve the nutritive value of animal.Marine benthic diatom is the important natural bait in stichopus japonicus, Bao Youti conversion of food habit stage, can promote attachment and the metamorphosis of young ginseng and Bao Miao.In the process of mass propgation benthic diatom, except the basic ecological factor such as illumination, temperature, it is required that the nutritive salt such as nitrogen, phosphorus, iron, silicon are similarly benthic diatom growth institute, important effect is had to its Nutrition and Metabolism, the quick growth of benthic diatom can be promoted, and present stage is also without any a kind of method or reagent, utilizes the above-mentioned characteristic of benthic diatom, growth promotion process and intervention are carried out to it.

Summary of the invention

The present invention is the above-mentioned deficiency in order to solve existing for prior art, proposes a kind of promote benthic diatom, the particularly end to dwell high efficiency nutritious salt formula that rhombus algae grows fast.

Technical solution of the present invention is: a kind of promote the end to dwell nutritive medium that rhombus algae grows fast, it is characterized in that: described nutritive medium comprises major element, trace element and VITAMIN, wherein major element comprises nitrogen, phosphorus, silicon, trace element comprises iron, zinc, copper, manganese, molybdenum, cobalt, and VITAMIN comprises vitamins B ₁, vitamins B ₁₂, vitamin H, and the concentration range of each component is as follows:

Nitrogen: 12.36-37.08 mg/L

Phosphorus: 2.27-2.84 mg/L

Silicon: 11.50-34.51 mg/L

Iron: 2.00-4.00 mg/L

Zinc: 0.65-6.54 μ g/L

Copper: 1.91-2.54 μ g/L

Manganese: 0-49.44 μ g/L

Molybdenum: 2.88-6.72 μ g/L

Cobalt: 2.95-5.89 μ g/L

Vitamins B ₁: 100-10000 μ g/L

Vitamins B ₁₂: 0.5-25.0 μ g/L

Vitamin H: 0.5-5.0 μ g/L.

In described nutritive medium, the concentration of each component is:

Nitrogen: 37.08 mg/L

Phosphorus: 2.84 mg/L

Silicon: 23.01 mg/L

Iron: 3.33 mg/L

Zinc: 6.54 μ g/L

Copper: 2.54 μ g/L

Manganese: 49.44 μ g/L

Molybdenum: 2.88 μ g/L

Cobalt: 29.5 μ g/L

Vitamins B ₁: 100 μ g/L

Vitamins B ₁₂: 0.5 μ g/L

Vitamin H: 0.5 μ g/L.

Compared with the existing technology, tool has the following advantages in the present invention:

Formula disclosed in this invention, to dwell the growth characteristics of rhombus algae to Different Nutrition salt demand the end of according to, reached the object promoting that purebred benthic diatom algae kind grows fast.Middle nutrition liquid collocation of the present invention rationally, effectively can promote the quick division growth of rhombus algae, impels it to absorb better and utilize various nutritive substance, accelerate the metabolism of rhombus frustule, improve its turnout; And in the nursery that can be applied to the marine economic animals such as stichopus japonicus as natural bait of rhombus algae and breeding process, strengthen its immunizing power and disease resistance, improve its nutritive value, to produce healthy green marine products economic animal seed, improve sea farming economic benefit and social benefit, the speed of growth therefore improving rhombus algae has larger meaning economically.Each Component Source in the present invention's formula is stablized, and with low cost, operation is simple and feasible, therefore can say that it has possessed market outlook widely, be particularly suitable for applying in aquaculture field.

Accompanying drawing explanation

Fig. 1 is embodiment of the present invention control group and the growth curve chart of the rhombus algae of different embodiment.

Embodiment

Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described.

The nutritive medium embodiment 1,2,3 of rhombus algae, as shown in table 1.

Table 1 rhombus algae formulation Example

Element	Control group	Embodiment 1	Embodiment 2	Embodiment 3
					N（mg/L）	12.36	37.08	12.36	37.08
P（mg/L）	1.14	2.84	2.27	2.84
					Si（mg/L）	2.30	11.50	34.51	23.01
Fe（mg/L）	0.65	4.00	2.00	3.33
					Zn（μg/L）	6.54	0.65	0.65	6.54
Cu（μg/L）	2.54	1.91	1.91	2.54
					Mn（μg/L）	49.44	27.47	0	49.44
Mo（μg/L）	2.88	6.72	6.72	2.88
					Co（μg/L）	2.95	5.89	2.95	2.95
VB 1 （μg/L）	100	8000	10000	100
					VB 12（μg/L）	0.5	25.0	5.0	0.5
VH （μg/L）	0.5	5.0	0.5	0.5

Each desired substance is added according to the concentration of control group in table 1 and embodiment 1,2,3 in the seawater of boiling sterilization, and inoculate the single rhombus algae of certain density wherein, cultivate after hungry 2d, incubation time is 10 d, and period, every 2 d measured the OD of a benthic diatom liquid ₆₈₀, to monitor the growth tendency of single rhombus algae, and calculate its specific growth rate, i.e. μ=(lnOD ₂-lnOD ₁)/(t ₂-t ₁), OD ₁and OD ₂be the optical density value at 680 nm places, t ₁and t ₂for the growth number of days of correspondence.

Simultaneously, inoculate the single rhombus algae of three groups of density same with control group, and use the Solution culture method rhombus algae of embodiments of the invention 1,2,3 respectively, cultivate after same hungry 2d, incubation time is 10d, and every 4 d add one time of nutrition salt (namely adding nutritive salt at 4d and 8d respectively), when the 10th d, results benthic diatom algae mud, weighs, calculates biomass.

result of use is as follows:

In the incubation time of 10 d, the growth curve chart of single rhombus algae as shown in Figure 1.Namely control group rhombus algae reaches exponential growth latter stage at the 6th d, and the rhombus algae that the embodiment of the present invention 1,2,3 is cultivated still exponentially grows at the 10th d, obviously extends its exponential phase of growth, short long successful.All obviously be greater than control group with the single rhombus algae growth tendency that the embodiment of the present invention 1,2,3 is cultivated, the single rhombus algae growth tendency that embodiment 3 is cultivated is the most obvious, short long best results.

The specific growth rate of table 2 rhombus algae

Specific growth rate	μ 2	μ 4	μ 6	μ 8	μ 10
						Control group	0.316	0.269	0.226	0.182	0.144
Embodiment 1	0.290	0.309	0.237	0.187	0.164
						Embodiment 2	0.343	0.338	0.245	0.188	0.163
Embodiment 3	0.463	0.365	0.270	0.209	0.169

Note: μ _nthe specific growth rate of representative when incubation time is the n-th d.

As shown in table 2, in the incubation time of 10 d, the single rhombus algae that embodiment 1,2,3 is turned out reaches maximum specific growth rate at the 6th d, simultaneously incubation time be the 2nd, 4,6,8,10 d time specific growth rate be all extremely significantly greater than control group, wherein the corresponding specific growth rate of embodiment 3 is all significantly higher than except embodiment 1,2(the 8th d), the short long successful of rhombus algae, culture effect is best.

The biomass of table 3 rhombus algae

Biomass (g/m 2）	Control group	Embodiment 1	Embodiment 2	Embodiment 3
					Rhombus algae	27.12	318.09	309.47	368.12

The biomass of the rhombus algae cultivated with embodiments of the invention 1,2,3 is as table 3, and compared with control group, biomass significantly improves, and is respectively 9.38,9.13,10.86 times of control group, and short long-acting fruit is extremely obvious.Wherein the biomass of embodiment 3 is apparently higher than embodiment 1,2, short long best results.

Claims (2)

1. promote the end a to dwell nutritive medium that rhombus algae grows fast, it is characterized in that: described nutritive medium comprises major element, trace element and VITAMIN, wherein major element comprises nitrogen, phosphorus, silicon, and trace element comprises iron, zinc, copper, manganese, molybdenum, cobalt, and VITAMIN comprises vitamins B ₁, vitamins B ₁₂, vitamin H, and the concentration range of each component is as follows:

Nitrogen: 12.36-37.08 mg/L

Phosphorus: 2.27-2.84 mg/L

Silicon: 11.50-34.51 mg/L

Iron: 2.00-4.00 mg/L

Zinc: 0.65-6.54 μ g/L

Copper: 1.91-2.54 μ g/L

Manganese: 0-49.44 μ g/L

Molybdenum: 2.88-6.72 μ g/L

Cobalt: 2.95-5.89 μ g/L

Vitamins B ₁: 100-10000 μ g/L

Vitamins B ₁₂: 0.5-25.0 μ g/L

Vitamin H: 0.5-5.0 μ g/L.

2. the nutritive medium that the rhombus algae that dwells at the bottom of promotion according to claim 1 grows fast, is characterized in that: in described nutritive medium, the concentration of each component is:

Nitrogen: 37.08 mg/L

Phosphorus: 2.84 mg/L

Silicon: 23.01 mg/L

Iron: 3.33 mg/L

Zinc: 6.54 μ g/L

Copper: 2.54 μ g/L

Manganese: 49.44 μ g/L

Molybdenum: 2.88 μ g/L

Cobalt: 29.5 μ g/L

Vitamins B ₁: 100 μ g/L

Vitamins B ₁₂: 0.5 μ g/L

Vitamin H: 0.5 μ g/L.