CN103403174B - 活性药物成分及其中间物的酶促合成 - Google Patents

活性药物成分及其中间物的酶促合成 Download PDF

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Publication number
CN103403174B
CN103403174B CN201180067928.6A CN201180067928A CN103403174B CN 103403174 B CN103403174 B CN 103403174B CN 201180067928 A CN201180067928 A CN 201180067928A CN 103403174 B CN103403174 B CN 103403174B
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pqq
dehydrogenase
compound
reaction
aldose
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Chinese (zh)
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CN103403174A (zh
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P·姆拉克
T·佐哈尔
M·欧什拉
G·科皮塔尔
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Lek Pharmaceuticals dd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C12P17/12—Nitrogen as only ring hetero atom containing a six-membered hetero ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pyrane Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
CN201180067928.6A 2010-12-20 2011-12-20 活性药物成分及其中间物的酶促合成 Expired - Fee Related CN103403174B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP10015774.2 2010-12-20
EP10015774A EP2465936A1 (en) 2010-12-20 2010-12-20 Enzymatic synthesis of statins and intermediates thereof
EP11168227 2011-05-31
EP11168227.4 2011-05-31
PCT/EP2011/073412 WO2012095244A2 (en) 2010-12-20 2011-12-20 Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof

Publications (2)

Publication Number Publication Date
CN103403174A CN103403174A (zh) 2013-11-20
CN103403174B true CN103403174B (zh) 2017-10-27

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CN201180067928.6A Expired - Fee Related CN103403174B (zh) 2010-12-20 2011-12-20 活性药物成分及其中间物的酶促合成

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US (1) US20130337485A1 (enExample)
EP (1) EP2655650B1 (enExample)
JP (1) JP6194251B2 (enExample)
CN (1) CN103403174B (enExample)
AU (1) AU2011355209B2 (enExample)
CA (1) CA2822331A1 (enExample)
WO (1) WO2012095244A2 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2210935A1 (en) * 2009-01-19 2010-07-28 Deinove Methods for isolating bacteria
CN110592249A (zh) * 2019-10-29 2019-12-20 山东省果树研究所 一种快速鉴定果园高效解磷细菌的特异性引物及其试剂盒、分离鉴定方法
CN113373187B (zh) * 2021-05-26 2023-11-10 江苏阿尔法药业股份有限公司 一种氮杂环化合物c27h30fno6的酶催化合成方法
CN114045243B (zh) * 2021-12-03 2023-12-26 山东天力药业有限公司 一种缩短生黑葡萄糖酸杆菌发酵周期的方法
CN116903543A (zh) * 2023-09-08 2023-10-20 重庆普佑生物医药有限公司 一种瑞舒伐他汀钙的制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007068498A1 (en) * 2005-12-15 2007-06-21 Isobionics B.V. Process for the preparation of delta lactones
WO2009092702A2 (en) * 2008-01-23 2009-07-30 Lek Pharmaceuticals D.D. ((2s,4r)-4,6-dihydroxytetrahydro-2h-pyran-2-yl)methyl carboxylate and process for the production thereof

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US3863170A (en) 1973-02-21 1975-01-28 Bendix Corp Thermally stable power amplifier
DE2401055A1 (de) 1974-01-10 1975-07-24 Horst Lindenmann Spielgeraet
US5567605A (en) 1994-11-21 1996-10-22 Uop Assay for D-allose using a NAD cofactor coupled D-allose dehydrogenase
WO2002034919A1 (en) 2000-10-27 2002-05-02 Roche Diagnostics Gmbh Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
US7414119B2 (en) 2002-09-20 2008-08-19 Verenium Corporation Aldolases, nucleic acids encoding them and methods for making and using them
JP4415282B2 (ja) 2003-03-24 2010-02-17 東洋紡績株式会社 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
EP1751287A2 (en) 2004-06-04 2007-02-14 DSMIP Assets B.V. Improved 2-deoxy-d-ribose 5-phosphate aldolases (deras) and the uses thereof
WO2006008132A1 (en) 2004-07-20 2006-01-26 Roche Diagnostics Gmbh Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion
ES2537106T3 (es) 2004-10-15 2015-06-02 F. Hoffmann-La Roche Ag Mutantes termoestables de la glucosa deshidrogenasa dependiente de quinona de pirroloquinolina
JP2006217811A (ja) 2005-02-08 2006-08-24 Toyobo Co Ltd 基質特異性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
CA2612293A1 (en) 2005-06-16 2006-12-21 Pfizer Inc. Process for producing atorvastatin, pharmaceutically acceptable salts thereof and intermediates thereof
CA2649135C (en) 2006-04-13 2013-11-19 F. Hoffmann-La Roche Ag Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase
US8183397B2 (en) 2007-04-03 2012-05-22 Lek Pharmaceuticals D.D. Synthesis of statins
US7947483B2 (en) * 2007-08-10 2011-05-24 Genomatica, Inc. Methods and organisms for the growth-coupled production of 1,4-butanediol
DE102008030435A1 (de) 2008-06-26 2010-01-07 Bayer Technology Services Gmbh Neuartige Varianten PQQ-abhängiger Glukosehydrogenase mit verbesserter Substratspezifität
WO2013068917A1 (en) * 2011-11-07 2013-05-16 Dr. Reddy's Laboratories Limited Processes for the production of a lactone statin intermediate by enzymatic oxidation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007068498A1 (en) * 2005-12-15 2007-06-21 Isobionics B.V. Process for the preparation of delta lactones
WO2009092702A2 (en) * 2008-01-23 2009-07-30 Lek Pharmaceuticals D.D. ((2s,4r)-4,6-dihydroxytetrahydro-2h-pyran-2-yl)methyl carboxylate and process for the production thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Oxidation of 1,4-alkanediols into gamma-lactones via gamma-lactols using Rhodococcus erythropolis as biocatalyst;Moreno-Horn等;《Journal of Molecular Catalysis》;20071026;第49卷(第1-4期);第24-27页 *
Stereoselective microbial/enzymatic oxidation of (exo,exo)-7-oxabicyclo[2.2.1]heptane-2,3-dimethanol to the corresponding chiral lactol and lactone;Patel R N等;《Enzyme and Microbial Technology》;19921001;第14卷(第10期);第778-784页 *

Also Published As

Publication number Publication date
JP2014501524A (ja) 2014-01-23
EP2655650A2 (en) 2013-10-30
WO2012095244A2 (en) 2012-07-19
CA2822331A1 (en) 2012-07-19
AU2011355209A1 (en) 2013-07-11
WO2012095244A3 (en) 2012-10-18
US20130337485A1 (en) 2013-12-19
JP6194251B2 (ja) 2017-09-06
EP2655650B1 (en) 2017-12-20
AU2011355209B2 (en) 2015-09-17
CN103403174A (zh) 2013-11-20

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