CN103396215B - Formula of hericium erinaceus stock compost and preparation method of compost - Google Patents
Formula of hericium erinaceus stock compost and preparation method of compost Download PDFInfo
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- CN103396215B CN103396215B CN201310310761.2A CN201310310761A CN103396215B CN 103396215 B CN103396215 B CN 103396215B CN 201310310761 A CN201310310761 A CN 201310310761A CN 103396215 B CN103396215 B CN 103396215B
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Abstract
The invention relates to a hericium erinaceus stock compost which is characterized in that corn kernels are used as main raw materials; cottonseed hulls and brans are used as auxiliary materials; and the pH value is regulate with potassium dihydrogen phosphate. The invention also includes a preparation method of the compost. Compared with the existing cottonseed hull compost, the compost provided by the invention has the following advantages: 1, hyphae can vigorously and quickly grow and are less possible to form sporophores (no sporophore is formed 20 days after the grown hyphae fully fill a bottle); 2, the transplant rate (transplant of cultivated species) is high, and the strains germinate quickly and grow vigorously after transplant; and 3, the auxiliary materials such as cottonseed hulls and brans are added, so that the moisture-containing capability of a culture medium is enhanced, and the uniform distribution of the moisture in the compost can be ensured simultaneously, thus preventing the corn kernels from age hardening and greatly prolonging the storage life of the strains.
Description
Technical field
Content of the present invention comprises Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material compatibility, and the making method of this culture material, belongs to edible mashroom cultivating technical field.
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers., sporophore is round and thick, is covered with needle-like bacterium thorn, the pole of figure like the head of monkey, so gain the name.The title that Hericium erinaceus (Bull. Ex Fr.) Pers. has " mountain delicacy ", it is rich in multivitamin, protein and polysaccharide body, immunity of organisms can be improved, repairing ulcer, inhibition tumor cell grows, and is control digestion method 3: in the present embodiment, Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material comprises following component and quality proportioning: major ingredient corn grain 90%, auxiliary material 10% (cotton seed hulls 85%, wheat bran 15%), be all the quality of dry-matter, adjusting pH value with potassium primary phosphate before sterilizing is 5.5-5.7.Making method is with reference to mode 1.
Mode 4 (control group): cotton seed hulls 78%, wheat bran 20%, terra alba 1%, sucrose 1%.
Spend widely different, output is often unstable, and especially in the large production of Hericium erinaceus (Bull. Ex Fr.) Pers., spawn culture difficulty is large, and conventional bacterium culture medium mycelia only long 2cm-5cm just very easily forms sporophore, is difficult to the bacterial classification that the amount of obtaining is of fine quality greatly.Lacking healthy and strong good quality strain is a great problem during Hericium erinaceus (Bull. Ex Fr.) Pers. produces, and the big area constraining Hericium erinaceus (Bull. Ex Fr.) Pers. is produced.
For solving this production difficult problem, the present invention uses corn grain for bacterium culture medium, by the acidity of potassium primary phosphate adjustment culture material, makes Hericium erinaceus (Bull. Ex Fr.) Pers. original seed, can reach desirable effect.
Summary of the invention
The present invention has following two aspects:
1, a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material compatibility
2, the making method of culture material
The technical solution adopted in the present invention is:
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material compatibility, there are following component and quality proportioning: major ingredient corn grain 85%-90%, auxiliary material 10%-15% (cotton seed hulls 85%, wheat bran 15%), be all the quality of dry-matter, adjusting PH with potassium primary phosphate before sterilizing is 5.5-5.7 (after sterilizing, the PH of culture material is 5.0-5.2).
Corn grain: without going mouldy, dry for subsequent use.
Wheat bran: the wheat cortex that wheat is separated in processing flour process.
Potassium primary phosphate: be agricultural potassium primary phosphate, the aqueous solution is in acid, and the pH value of 1% potassium dihydrogen phosphate is 4.6.
The making method of culture material, comprises the steps:
1. first cleaned by corn grain clear water, soak 15h-20h, then corn grain is put into pot, lower poach to endosperm deposits the white heart of a line, drains away the water;
2. mixed thoroughly by auxiliary material (cotton seed hulls and wheat bran) siccative, then add potassium dihydrogen phosphate aqueous solution, limit edged is mixed, and makes the water content of culture material remain on 63%-65%, and PH is 5.5-5.7;
3. corn grain and auxiliary material are stirred;
4. bottle, beat and plant bacterium hole: culture material is loaded 1100ml × 78mmPP Plastic Bottle, stamp and plant bacterium hole, planting aperture, bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band;
5. sterilizing, cooling: culture material is carried out autoclaving, first sterilizing 1h at 100 DEG C, then rises to 121 DEG C of sterilizing 2.0h, naturally cools to less than 25 DEG C after sterilizing.
Culture material is after the female kind of access, and culture temperature controls at 20 DEG C-22 DEG C, and humid control is at 60%-75%, and through the cultivation of 18d-20d, mycelia covers with bottle, namely can be used to make cultivar.
The present invention take corn grain as main raw material, with cotton seed hulls, wheat bran for auxiliary material, adjusts pH value with potassium primary phosphate, makes Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material.Compared with existing cotton seed hulls culture material, its advantage is: one is that mycelial growth is strong, rapid, not easily forms sporophore (mycelia is 20d after covering with bottle, does not all form sporophore); Two is that switching rate (switching cultivar) is high, and after switching, bacterial classification sprouting is fast, growing way is good; Three is add cotton seed hulls, wheat bran auxiliary material, improves the ability that substratum holds moisture, can ensure to expect that interior moisture distribution is even simultaneously, avoids maize seed grain age hardening, the fungi preservation phase is extended greatly.
Embodiment
Mode 1: in the present embodiment, Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material comprises following component and quality proportioning: major ingredient corn grain 85%, auxiliary material 15% (cotton seed hulls 85%, wheat bran 15%), be all the quality of dry-matter, adjusting pH value with potassium primary phosphate before sterilizing is 5.5-5.7.
Making method:
1. first cleaned by corn grain clear water, soak 15h-20h, then corn grain is put into pot, lower poach to endosperm deposits the white heart of a line, drains away the water;
2. mixed thoroughly by auxiliary material (cotton seed hulls and wheat bran) siccative, then add potassium dihydrogen phosphate aqueous solution, limit edged is mixed, and makes the water content of culture material remain on 63%-65%, and pH value is 5.5-5.7;
3. corn grain and auxiliary material are stirred;
4. bottle, beat and plant bacterium hole: culture material is loaded 1100ml × 78mmPP Plastic Bottle, stamp and plant bacterium hole, planting aperture, bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band;
5. sterilizing, cooling: culture material is carried out autoclaving, first sterilizing 1h at 100 DEG C, then rises to 121 DEG C of sterilizing 2.0h, naturally cools to less than 25 DEG C after sterilizing.
Culture material is after the female kind of access, and culture temperature controls at 20 DEG C, and humid control is 60%, and through the cultivation of 18d, mycelia covers with bottle, namely can be used to make cultivar.
Mode 2: in the present embodiment, Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material comprises following component and quality proportioning: major ingredient corn grain 88%, auxiliary material 12% (cotton seed hulls 85%, wheat bran 15%), be all the quality of dry-matter, adjusting pH value with potassium primary phosphate before sterilizing is 5.5-5.7.Making method is with reference to mode 1.
Mode 3: in the present embodiment, Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material comprises following component and quality proportioning: major ingredient corn grain 90%, auxiliary material 10% (cotton seed hulls 85%, wheat bran 15%), be all the quality of dry-matter, adjusting pH value with potassium primary phosphate before sterilizing is 5.5-5.7.Making method is with reference to mode 1.
Mode 4 (control group): cotton seed hulls 78%, wheat bran 20%, terra alba 1%, sucrose 1%.
From following table, the sprout time of the hericium mycelium of corn grain culture material is 18h, and mycelial growth is dense, pure white, sturdy, and full bottle Time transfer receiver shortens 12d-14d according to group; Compare in bacterial classification resistance of aging, the original seed utilizing corn grain to make is not easily aging, and control group mycelia is less than bottle, and bacterial classification is aging.
Table four group culture material is on the impact of Hericium erinaceus (Bull. Ex Fr.) Pers. original seed mycelial growth
| Mode | Sprout time (h) | Mycelial density | Robustness | Full bottle time (d) | Digestion time (d) |
| 1 | 18 | ++++ | Pure white, sturdy | 18 | 50 |
| 2 | 18 | ++++ | Pure white, sturdy | 19 | 50 |
| 3 | 18 | ++++ | Pure white, sturdy | 20 | 52 |
| 4 | 48 | +++ | Greyish white, rarer | 32 | 24 |
Note: mycelial density with ++, +++ with ++++represent ,+number more multilist shows that mycelial density is higher.
Claims (5)
1. a Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material, it is characterized in that containing, for example lower component and quality proportioning: major ingredient corn grain 85%-90%, auxiliary material 10%-15%, in auxiliary material, cotton seed hulls accounts for 85%, wheat bran accounts for 15%, be all the quality of dry-matter, adjusting pH with potassium primary phosphate before sterilizing is 5.5-5.7, and after sterilizing, the pH of culture material is 5.0-5.2; The making method of described culture material is:
1. first cleaned by corn grain clear water, soak 15h-20h, then corn grain is put into pot, lower poach to endosperm deposits the white heart of a line, drains away the water;
2. cotton seed hulls and wheat bran siccative are mixed thoroughly, then add potassium dihydrogen phosphate aqueous solution, limit edged is mixed, and makes the water content of culture material remain on 63%-65%, and pH is 5.5-5.7;
3. corn grain and auxiliary material are stirred;
4. bottle, beat and plant bacterium hole: culture material is loaded 1100ml × 78mmPP Plastic Bottle, stamp and plant bacterium hole, planting aperture, bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band;
5. sterilizing, cooling: culture material is carried out autoclaving, first sterilizing 1h at 100 DEG C, then rises to 121 DEG C of sterilizing 2.0h, naturally cools to less than 25 DEG C after sterilizing.
2. Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material according to claim 1, is characterized in that described corn grain is the corn without going mouldy.
3. Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material according to claim 1, is characterized in that the wheat cortex that described wheat bran is separated in processing flour process for wheat.
4. Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material according to claim 1, is characterized in that described potassium primary phosphate, is agricultural potassium primary phosphate, and the aqueous solution is in acid, and the pH value of 1% potassium dihydrogen phosphate is 4.6.
5. Hericium erinaceus (Bull. Ex Fr.) Pers. Primary spawn material according to claim 1, after it is characterized in that the made female kind of culture material access, culture temperature controls at 20 DEG C ~ 22 DEG C, humid control is 60% ~ 75%, through the cultivation of 18d-20d, mycelia covers with bottle, namely can be used to make cultivar.
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