CN103393768A - Rhizoma Polygoni Cuspidati and Adenophora stricta combined medicine for cough relieving and panting calming, and preparation and application thereof - Google Patents
Rhizoma Polygoni Cuspidati and Adenophora stricta combined medicine for cough relieving and panting calming, and preparation and application thereof Download PDFInfo
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Abstract
The invention provides a Rhizoma Polygoni Cuspidati and Adenophora stricta combined medicine for cough relieving and panting calming. The aqueous extract of the combined medicine has a good activity in the cough relieving, panting calming, phlegm eliminating, swelling subduing, inflammation prevention and the like, and the 70% alcohol extract of the combined medicine has a good activity in immunity enhancement.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation, relate in particular a kind of tiger ginseng relieving cough and asthma composition of medicine, preparation and application thereof.
Technical background
Traditional medicine thinks, chronic bronchitis belong in exopathogen more invade, due to the endogenous cough, and eating and drinking without temperance, dysfunction of the spleen in transportation, give birth to wet poly-expectorant; Internal organs imbalances, lung are defended deficiency, cause expectorant erosion resistance lung, show as the diseases such as cough, expectoration, asthma, and controlling should detoxicating, relieving inflammation, relieving cough and resolving phlegm, relieving asthma.Modern medicine thinks, chronic bronchitis has infection, chemical factors, climate change, irritated factor etc. all can cause chronic bronchitis more.Infection control, eliminating phlegm and relieving cough are often adopted in treatment, spasmolytic is relievingd asthma, and no matter is traditional medicine or modern medicine, and the treatment of this disease has obvious concordance.
Summary of the invention
For addressing the above problem, the invention provides a kind of tiger ginseng relieving cough and asthma composition of medicine, preparation and application thereof; Traditional medicine thinks that the cause of disease of chronic cough, chronic bronchitis, tracheitis and pathogenesis are: the diseases caused by exogenous pathogenic factor pathogen, due to the endogenous cough, as eating and drinking without temperance, dysfunction of the spleen in transportation, give birth to wet poly-expectorant; Internal organs imbalances, lung are defended deficiency, and the chamber of causing rises and respectfully falls unbalancely, causes the expectorant erosion to hinder lung, shows as the diseases such as cough, expectoration, asthma, and controlling should detoxicating, relieving inflammation, relieving cough and resolving phlegm, relieving asthma, to take stopgap measures.
Due to considering that mostly chronic tracheitis, bronchitis are that repeatedly exopathogen invades, thus in side take detoxicating, relieving inflammation as main; In side, Rhizoma Polygoni Cuspidati, Fructus Cnidii are the detoxicating, relieving inflammation key medicine, and modern pharmacological research shows, Rhizoma Polygoni Cuspidati, Fructus Cnidii all have significantly antibacterial and antiinflammatory action.Radix Adenophorae is the cough-relieving key medicine, mainly contains β ?Sitosterols material, the tool antitussive effect, and Radix Glycyrrhizae is tool detoxifcation and coordinating the actions of various ingredients in a prescription effect, the obvious antiinflammation of tool and the effect of corticoid sample also.
In the many companions of we card exopathogen, invades, endogenous cough for a long time, and then cause lung to defend deficiency, expectorant erosion resistance lung.Common deficiency syndrome, be mingled with excess pathogen.In side, Rhizoma Polygoni Cuspidati is bitter, sour, cool, and clearing away heat-damp and promoting diuresis, promoting blood circulation and detoxication, to effect a permanent cure, are monarch drug; The Fructus Cnidii acrid, bitter, warm, warming the kidney to activate YANG, removing dampness and killing parasites, help the Rhizoma Polygoni Cuspidati detoxification and promoting urination, thinks ministerial drug; Radix Adenophorae is sweet, cool, lung moistening, eliminate the phlegm, cough-relieving to be to take stopgap measures, and is adjuvant drug; Radix Glycyrrhizae is sweet, flat, the spleen invigorating eliminating dampness by diuresis, expelling phlegm for arresting cough, detoxifcation, help the monarch and his subjects' eliminating damp, detoxifying, close Radix Adenophorae and share, expelling phlegm for arresting cough, the coordinating the actions of various ingredients in a prescription effect of holding concurrently, therefore for making.All medicines share, the effect of have detoxifcation, antiinflammatory, dampness removing, cough-relieving, eliminating the phlegm, relievining asthma, treating both the principal and secondary aspects of a disease, all card spontaneous recovery.
The crude drug that adopts in we: Radix Adenophorae is traditional cough-relieving key medicine, and Radix Glycyrrhizae is also often used; Rhizoma Polygoni Cuspidati tool clearing away heat-damp and promoting diuresis, control the blood detoxicating functions, is traditional detoxicating, relieving inflammation medicine; Fructus Cnidii tool warming the kidney to activate YANG, the removing dampness and killing parasites effect.Modern pharmacological research shows, in Radix Adenophorae De β ?the obvious antitussive action of sitosterol tool; The effect of Radix Glycyrrhizae tool class cortical hormone sample, namely have an antiinflammation; The obvious antibacterial action of Rhizoma Polygoni Cuspidati tool; Fructus Cnidii has parahormone sample function, has anti-allergic effects, has antibacterial action concurrently; Application of composite, have detoxicating, relieving inflammation, a cough and asthma relieving efficacy.Traditional treatment chronic bronchitis, chronic tracheitis, adopt palliative more, namely take relieving cough and asthma, eliminate the phlegm as main, accompany by simultaneously the spleen invigorating eliminating dampness by diuresis, the medicines such as warming the kidney to promote diuresis are to solve expectorant erosion resistance lung problem, less employing detoxicating, relieving inflammation Drug therapy.
This Therapeutic Method adopts take detoxicating, relieving inflammation as main, and the double relieving cough and asthma of controlling eliminates the phlegm.This Therapeutic Method principle namely meets Traditional Chinese medical theory, also with modern medical theory, matches, and chronic tracheitis, bronchitis are many with infecting (being to invade in exopathogen), and are paathogenic factor.Therefore, this prescription has novelty preferably, and traditional medicine and modern medical theory are all supported its feasibility simultaneously.
A kind of tiger ginseng relieving cough and asthma composition of medicine, the active component of composition of medicine is comprised of the extract of Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae.
The weight ratio of described Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae is 4-12:10-16:4-9:2-6.
The weight ratio of described Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae is 17:26:13:9.
Described tiger ginseng relieving cough and asthma composition of medicine is applied in preparing antitussive medicine.
Described tiger ginseng relieving cough and asthma composition of medicine is applied in preparing suppressing panting calming medicine.
Described tiger ginseng relieving cough and asthma composition of medicine is applied in preparing expelling phlegm drugs.
Described tiger ginseng relieving cough and asthma composition of medicine is applied in preparing the functions of detumescence, relieving inflammation medicine.
Described tiger ginseng relieving cough and asthma composition of medicine is applied in preparing the enhancing immunity medicine.
A kind of preparation of tiger ginseng relieving cough and asthma composition of medicine, become capsule by above-mentioned tiger ginseng relieving cough and asthma composition of medicine preparation.
Useful technique effect of the present invention is: the invention provides a kind of tiger ginseng relieving cough and asthma composition of medicine, the water extract of this composition of medicine in antitussive, relieving asthma, eliminate the phlegm, the aspect such as functions of detumescence, relieving inflammation has good activity; 70% ethanol extract of this composition of medicine has good activity aspect enhancing immunity.
The specific embodiment
The preparation method of embodiment 1 tiger ginseng relieving cough and asthma composition of medicine
Take Rhizoma Polygoni Cuspidati 850g, Fructus Cnidii 1300g, Radix Adenophorae 650g, Radix Glycyrrhizae 450g; Merge above-mentioned medical material, pulverize, with 5 times, 3 times and 2 times water of weight, reflux respectively, each 30min, merge aqueous solution, filters, and is condensed into extractum, as tiger ginseng relieving cough and asthma group 1;
Take Rhizoma Polygoni Cuspidati 400g, Fructus Cnidii 1600g, Radix Adenophorae 900g, Radix Glycyrrhizae 200g; Merge above-mentioned medical material, pulverize, with 5 times, 3 times and 2 times water of weight, reflux respectively, each 30min, merge aqueous solution, filters, and is condensed into extractum, as tiger ginseng relieving cough and asthma group 2;
Take Rhizoma Polygoni Cuspidati 1200g, Fructus Cnidii 1000g, Radix Adenophorae 400g, Radix Glycyrrhizae 600g; Merge above-mentioned medical material, pulverize, with 5 times, 3 times and 2 times water of weight, reflux respectively, each 30min, merge aqueous solution, filters, and is condensed into extractum, as tiger ginseng relieving cough and asthma group 3;
Take Rhizoma Polygoni Cuspidati 850g, Fructus Cnidii 1300g, Radix Adenophorae 650g, Radix Glycyrrhizae 450g; Merge above-mentioned medical material, pulverize, with 500mL70% ethanol, infiltrate, use the 5000mL70% ethanol percolation, the percolation flow velocity is 2mLmin
-1, merge percolate, filter, be condensed into extractum, as tiger ginseng relieving cough and asthma group 4.
The antitussive effect of embodiment 2 tiger ginseng relieving cough and asthma composition of medicine is investigated
1. tiger ginseng relieving cough and asthma composition of medicine draws the impact of coughing on mice ammonia
By the healthy mice of body weight 20 ± 2g, with the cough of ammonia Induced by Stimulation, it is qualified for screening that the number of times of coughing in 3min reaches more than three times.Get the qualified mice of screening, be divided at random six groups.Tiger ginseng relieving cough and asthma composition of medicine 5.5 crude drugs that the other ig embodiment of drug component obtains/kg, positive controls SC Codeine Hydrochloride 60mg/kg, matched group is ig equivalent distilled water.Once a day, continuous three days (except Codeine Hydrochloride), last administration 1h(Codeine Hydrochloride 30min) after, by screening technique, undertaken, add up cough number of times in every group of mice 3min, result is checked with statistic software SPSS 10.0 one factor analysis of variance methods.Experimental result is as shown in table 1.
Table 1 tiger ginseng relieving cough and asthma composition of medicine draws the impact of coughing on mice ammonia
With matched group than * P<0.05 * * P<0.01
2. tiger ginseng relieving cough and asthma composition of medicine causes the impact of coughing on the Cavia porcellus citric acid
Get body weight 200～250g Cavia porcellus, be placed in the airtight bell jar of 4L volume, with 400mmHg pressure, spray into 52.5% citric acid, spraying 1min, guinea pig cough's number of times in 5min >=person more than 10 times is qualified for screening.
The qualified Cavia porcellus of screening is divided into to six groups (n=10) at random, the other ig tiger of drug component ginseng relieving cough and asthma composition of medicine 4.5 crude drugs/kg, positive controls SC Codeine Hydrochloride 40mg/kg, matched group ig equivalent distilled water.Be administered once.1h after medicine, test by pre-selection method, records guinea pig cough's incubation period and 5min cough number of times (when 5min did not occur coughing, incubation period is the 5min meter on schedule).Result is checked with statistic software SPSS 10.0 one factor analysis of variance methods.Result is as shown in table 2.
Table 2 tiger ginseng relieving cough and asthma composition of medicine causes the effect of coughing to the Cavia porcellus citric acid
With matched group than * P<0.05 * * P<0.01
Table 2 shows: tiger ginseng relieving cough and asthma composition of medicine can obviously resist the guinea pig cough that citric acid causes, and each dosage all can significant prolongation cough latent period (P<0.05-0.01).
Embodiment 3 tiger ginseng relieving cough and asthma composition of medicine antiasthmatic effects are investigated
1. tiger ginseng relieving cough and asthma composition of medicine causes the impact of Experimental Asthma In Guinea-pigs on histamine
Get 200g left and right Cavia porcellus, in the airtight bell glass of 4L volume, with the 400mmHg constant voltage, spray into 2% acetylcholine and 0.4% 2ml:4ml of histamine phosphate mixed liquor, spray 15S at every turn.Recording in 6min Cavia porcellus, to occur that the tic of panting property is less than 150S person incubation period qualified for screening.
The qualified Cavia porcellus of screening is divided into to six groups (n=10) at random, 4 groups of tiger ginseng relieving cough and asthma composition of medicine 4.5g crude drug/kg that the other ig embodiment 1 of drug component obtains, positive controls ip aminophylline 0.1g/kg, matched group ig equivalent distilled water.Be administered once.In 1h(aminophylline 30min after medicine) after, by screening technique, test.Record the incubation period that animal is had a convulsion in 6min (person in 6min, do not occur, depending on be incubation period 6min).Result is respectively organized incubation period with statistic software SPSS 10.0 statistics.Result of the test is as shown in table 3.
Table 3 tiger ginseng relieving cough and asthma composition of medicine draws the effect of breathing heavily to Guinea Pig Histamine
With matched group, compare * P<0.05 * * P<0.01
Table 3 shows: tiger ginseng relieving cough and asthma composition of medicine group all can obviously extend histamine and cause the incubation period that Cavia porcellus twitches, and panting of bringing out of histamine had to significant protective effect (P<0.05).
2. egg protein is caused to the impact of Cavia porcellus allergic asthma
Get 200g left and right Cavia porcellus, prior to thigh lateral muscle meat injection 4% egg protein normal saline 0.1ml(mono-side), every guinea pig intraperitoneal injection adsorbs whooping cough combined vaccine 1ml sensitized animal simultaneously.Second day is divided into six groups at random by animal, medicine group ig tiger ginseng relieving cough and asthma composition of medicine 4.5 crude drugs/kg, positive controls ig ketotifen sheet 1mg/kg, matched group ig equivalent distilled water, every day 1 time, continuous 14 days.After the last medicine 1 hour, with 4% egg protein normal saline, carry out constant-pressure atomization 20s, then observe Cavia porcellus in 6min and incubation period, the cough number of times that accelerated breathing, dyspnea, tic are fallen and suffered a shock occur and the shock animals number occurs.Result is respectively with statistic software SPSS 10.0 one factor analysis of variance method checks.Result of the test is as shown in table 4.
Table 4 tiger ginseng relieving cough and asthma composition of medicine causes the impact of Cavia porcellus allergic asthma on egg protein
With matched group, compare * P<0.05, * * P<0.01
By as seen from Table 4, tiger ginseng relieving cough and asthma composition of medicine causes the Cavia porcellus allergic asthma to egg protein significant protective effect.Can obviously extend dyspnea that egg protein causes and the incubation period of shock, significantly resist the guinea pig cough that egg protein causes, reduce shock animals number (P<0.05-0.01).
Embodiment 3 tiger ginseng relieving cough and asthma composition of medicine phlegm-dispelling functions are investigated
1. the impact of tiger ginseng relieving cough and asthma composition of medicine on rat capillary tube method expectoration amount
Choosing body weight is 200 ± 20g healthy rat, with after pentobarbital sodium anesthesia, faces upward position and fixedly keeps flat, and cuts skin of neck, separates trachea, at correct position, inserts a capillary glass tube, makes juice be discharged into capillary tube.Juice column length in capillary tube of every 30 minutes records, after 2h, press the random administration of table 5 dosage, after medicine, with method, records juice column length in the 2h capillary tube again.Statistical, per hour average secretory volume is as normal value before administration, and after administration, average per hour secretory volume, as be 170% of normal value, thought phlegm-dispelling functions is arranged.According to said method estimate the expectorant effect of tiger ginseng relieving cough and asthma composition of medicine.Result of the test is as shown in table 5.
The impact of table 5 tiger ginseng relieving cough and asthma composition of medicine on rat capillary tube method expectoration amount
Annotate: * represent per hour expectoration amount after medicine be before medicine more than 170%
By as seen from Table 5, with matched group, compare, tiger ginseng relieving cough and asthma composition of medicine has significant phlegm-dispelling functions to rat.
2. the impact of the phenol red method of mice being eliminated the phlegm
Get body weight 20 ± 2g healthy mice, ♀ ♂ half and half, be divided into six groups at random.The other gavage tiger of drug component ginseng relieving cough and asthma composition of medicine 5.5g crude drug/kg, positive controls ig ammonium chloride 1.0g/kg, matched group ig equivalent distilled water.Once a day, for three days on end, 30min after the last administration, the phenol red 0.1ml/10g of every Mus ip0.6%, put to death after ip30min, separates trachea, with 38 ℃ of 5%NaHCO
3Solution 2ml divides the flushing trachea three times.Collect flushing liquor, in 546nm wavelength colorimetric, result is checked with statistic software SPSS 10.0 one factor analysis of variance methods with 72 type spectrophotometers.Result of the test is as shown in table 6.
The impact that table 6 tiger ginseng relieving cough and asthma composition of medicine eliminates the phlegm on the phenol red method of mice
With matched group, compare * P<0.05 * * P<0.01
Table 6 shows: the big or middle dosage of tiger ginseng relieving cough and asthma composition of medicine can significantly promote phenol red discharge in the mice trachea, compared with the control, obvious expectorant effect (P<0.05) is arranged.
Embodiment 4 tiger ginseng relieving cough and asthma composition of medicine antiinflammatory actions are investigated
1. the impact of tiger ginseng relieving cough and asthma composition of medicine on the swollen hypertrophy of rat granuloma
Get 72 of body weight 200 ± 20g healthy rats, under ether light anaesthesia aseptic condition, make abdominal incision, respectively implant a 10mg cotton balls of 0.2ml penicillin is arranged through autoclaving, oven dry dropping the left and right groin is subcutaneous.After performing the operation, rat is divided into six groups at random, starts administration, medicine group ig tiger ginseng relieving cough and asthma composition of medicine 4.5g crude drug/kg, positive controls ig prednisone 25mg/kg, and matched group ig equivalent distilled water, once a day, successive administration 10 days.After last administration 1h, put to death rat, peel off and take out the cotton balls granulation tissue, oven dry is weighed, and deducts the raw cotton ball weight, is the granuloma net weight, and result is checked with statistic software SPSS 10.0 one factor analysis of variance methods.Respectively organize granuloma weight; Result of the test is as shown in table 7.
The inhibitory action of table 7 tiger ginseng relieving cough and asthma composition of medicine to the swollen hypertrophy of rat granuloma
With matched group than * P<0.05 * * P<0.05
As seen from Table 7: with matched group, compare, tiger ginseng relieving cough and asthma composition of medicine group has significant inhibitory action (P<0.05-0.01) to the swollen hypertrophy of rat granuloma.
2. on the impact of mice chronic bronchitis
Get 80 of body weight 20 ± 2g healthy mices, wherein make normal control for 14.All the other mice moulding: mice is put in to airtight carton, lights two medicated cigarettes, after sootiness, take out and be placed in 2-8 ℃ of refrigerator at once, freezing 30min, every day at the upper and lower noon is respectively once.After 20 days, get minority moulding mice trachea, lung and the inspection of normal mouse malingering relatively, after determining the modeling success, the moulding mice is divided into to six groups at random: tiger ginseng relieving cough and asthma composition of medicine (the present embodiment is referred to as tiger ginseng 1,2,3,4) 5.5g crude drug/kg, positive controls ig dexamethasone 0.02g/kg that the other ig embodiment 1 of drug component prepares, the equal ig equivalent of model group and matched group distilled water.Be administered once every day, and continuous 10 days, after last administration 1h, weighing Mouse Weight execution, got lung and weigh, with statistic software SPSS 10.0 one factor analysis of variance methods checks.Get lung, trachea is fixed in 10% formalin, makes pathology and detects, mainly observe four indexs: chronic inflammation cellular infiltration, bronchial mucosa epithelial proliferation, bronchial mucosa necrosis come off, goblet cell increases.Each index of every Mus is with " slightly " or " obviously " judgement, and each indicator and model group compares, X 2 test.Result of the test is as shown in table 8,9.
The impact of table 8 tiger ginseng relieving cough and asthma composition of medicine on the heavy coefficient of Chronic Bronchitis Mice lung
With matched group, compare * P<0.05
The impact that table 9 tiger ginseng relieving cough and asthma composition of medicine changes Chronic Bronchitis Mice lung, bronchus pathology
With model group, compare * P<0.05**P<0.01; With matched group, compare
△ △P<0.01
(1) table 8 shows: with model group, compare, tiger ginseng relieving cough and asthma composition of medicine has no significant effect the heavy coefficient of Chronic Bronchitis Mice lung.
(2) as seen from Table 9: relatively, each sick inspection index has significant difference for model group and normal group.And big or middle dose of group of tiger ginseng and model group are relatively, and the sick inspection of each of mice chronic bronchitis index all has clear improvement, and especially mice trachea inflammatory cell infiltration, bronchial mucosa epithelial proliferation and goblet cell is increased, and significant inhibitory action is arranged.Illustrate that tiger ginseng relieving cough and asthma composition of medicine has the effect of remarkable inhibition mice chronic bronchitis.
3. tiger ginseng relieving cough and asthma composition of medicine xylol causes the impact of mice ear
Get 60 of body weight 20 ± 2g healthy mices, male and female half and half, be divided into six groups at random.The other ig tiger of drug component ginseng relieving cough and asthma composition of medicine 5.5 crude drugs/kg, positive controls ig prednisone 5mg/kg, matched group ig equivalent distilled water.Once a day, continuous three days, after last administration 1h, at every Mus auris dextra, only being coated with dimethylbenzene 20 μ L/, after 20min, putting to death, is 8mm with diameter, card punch takes off two auricles and weighs, and take the difference of two auricle weight as swelling, result is with statistic software SPSS 10.0 one factor analysis of variance methods checks.Shown in result of the test table 10.
Table 10 tiger ginseng relieving cough and asthma composition of medicine xylol causes the effect of mice ear
With matched group, compare * P<0.05
By as seen from Table 7, with matched group, to compare, tiger ginseng relieving cough and asthma composition of medicine xylol causes mice ear certain antagonism, but effect not significantly (P > 0.05).
4. the impact of tiger ginseng relieving cough and asthma composition of medicine on the mice capillary permeability
Get body weight 20 ± 2g mice, be divided at random six groups, respectively ig tiger ginseng relieving cough and asthma composition of medicine 5.5g crude drug/kg, prednisone 5mg/kg, equivalent distilled water.Once a day, continuous three times.After last administration 1h, in the tail vein, inject 0.5% Yi Wensilan normal saline solution 0.1ml/10g body weight, melted paraxylene 0.03ml/ is only on the skin of unhairing of position, mouse web portion center immediately, after 20min, put to death, cut the painted skin of abdominal part, put into 7:3 acetone and mixed liquor of normal saline 4ml, soak 48h, centrifuging and taking supernatant, 610nm wavelength colorimetric.With statistic software SPSS 10.0 one factor analysis of variance method checks, statistics is respectively organized optical density (OD) value.Experimental result is as shown in table 11.
The impact of table 11 tiger ginseng relieving cough and asthma composition of medicine on the mice capillary permeability
With matched group than * P<0.05
Table 11 demonstration: with matched group, compare, the obvious antagonism of mice vascular permeability increase nothing that tiger ginseng relieving cough and asthma composition of medicine xylol causes (P > 0.05).
The impact of embodiment 5 tiger ginseng relieving cough and asthma composition of medicine on the nonspecific immunity function
1. normal mouse reticuloendothelial system (RES) is engulfed to the impact of carbon clearance ability in blood flow
Get 60 of body weight 20 ± 2g healthy mices, ♀ ♂ half and half, be divided into six groups at random, the other ig tiger of drug component ginseng relieving cough and asthma composition of medicine 5.5 crude drugs/kg, positive controls ig levamisole 80mg/kg, matched group ig equivalent distilled water.Every day ig once, continuous 7 days, after the last medicine 1 hour, every Mus tail iv india ink 0.1ml/10g, respectively got eye socket blood 20 μ l in 30s, 5min after iv, is placed in 2ml0.5%NaHCO is housed
3In vitro, with 72 type spectrophotometer wavelength 670nm colorimetrics, read optical density, result is checked with statistic software SPSS 10.0 one factor analysis of variance methods.Result of the test is as shown in table 12.
Table 12 is engulfed the impact of carbon clearance ability in blood on mice RES
With matched group, compare * P<0.05
By as seen from Table 12, with matched group, compare, tiger ginseng relieving cough and asthma composition of medicine has certain effect to strengthening the normal mouse RES carbon clearance ability in blood flow of engulfing, but DeGrain (P > 0.05), do not cause the change of Mouse Liver, spleen weight.
2. the impact of tiger ginseng relieving cough and asthma composition of medicine on the generation of normal mouse hemolytic antibody
Get 50 of body weight 20 ± 2g healthy mices, ♀ ♂ half and half, be divided into six groups at random.Each group is ig tiger ginseng relieving cough and asthma composition of medicine 5.5g crude drug/kg, levamisole 80mg/kg, equivalent distilled water respectively.Every day ig once, continuous 10 days.Administration the 3rd day, every Mus ip4% chicken erythrocyte suspension 0.2ml, carry out immunity to animal.After last administration 1h, eye socket is got blood 20 μ l, adds in the 1ml normal saline and mixes, then add the mixed liquor of chicken erythrocyte suspension, 0.5ml normal saline and the fresh guinea pig serum 1:15 of 0.5ml5%.After mixing, put into 37 ℃ of waters bath with thermostatic control 30 minutes, stopped reaction in refrigerator, centrifugal, get supernatant 1ml and add 3ml Dou Shi liquid, after standing 10 minutes, in 72 type spectrophotometer 540nm wavelength colorimetrics, measure optical density (OD), OD value * 1000 are the serum hemolysin value.Result is checked with statistic software SPSS 10.0 one factor analysis of variance methods.Result of the test is as shown in table 13.
The impact that table 13 tiger ginseng relieving cough and asthma composition of medicine generates the mice hemolytic antibody
With matched group, compare * * P<0.01
As seen from Table 13: tiger ginseng relieving cough and asthma composition of medicine generates certain promotion is arranged the normal mouse hemolytic antibody, but acts on not obvious (P > 0.05).
The preparation of embodiment 6 tiger ginseng relieving cough and asthma composition of medicine
Supplementary material constitutive material: tiger ginseng relieving cough and asthma composition of medicine; Adjuvant: medical starch, calcium hydrogen phosphate, micropowder silica gel (additional) wetting agent: certain density alcoholic solution.
The relieving cough and asthma composition of medicine granular mass criterion (in Table 14) of tiger ginseng.
Table 14 tiger ginseng relieving cough and asthma composition of medicine granular mass evaluation criterion (total points 100)
Tiger is joined to relieving cough and asthma composition of medicine, medical starch, calcium hydrogen phosphate and is placed in trough type mixing machine and mixes, add certain density alcoholic solution as wetting agent, to mix thoroughly and make suitable soft material in right amount, check soft material quality make it to reach hands pinch agglomerating, namely the faling apart and be advisable of strandings.With 30 order nylon sieve series wet granulars, 55 ℃ are about 1.5% with interior aeration-drying to moisture, and with 2O mesh sieve granulate, dry granular adds micropowder silica gel, at " v " type mixer, always is mixed even, after the intermediate products passed examination, with Autocapsulefillingmachine, fills.
Orthogonal test and result: take tiger ginseng relieving cough and asthma composition of medicine granular mass evaluation criterion as investigating index, by the preparation of above-mentioned tiger ginseng relieving cough and asthma composition of medicine capsule, operate, adopt orthogonal experiment, with calcium hydrogen phosphate use amount (A), micropowder silica gel use amount (B), 3 factors of concentration of alcohol (c), each factor is chosen 3 levels and is tested, and the factor level of formulating is selected L
9(4
3) orthogonal table arranges test, each factor is fetched water and is put down in Table 15, experimental result under different factor levels (in Table 16), variance analysis (in Table 17).
Table 15 empirical factor water-glass
The L of table 16 preparation technology orthogonal experiment
9(3
4) table
Table 17 analysis of variance table
As can be seen from Table 17.The impact size difference A of each factor on tiger ginseng relieving cough and asthma composition of medicine capsule content uniformity > B > C.Calcium hydrogen phosphate use amount, micropowder silica gel use amount have significant to the impact of tiger ginseng relieving cough and asthma composition of medicine capsule content uniformity, and optimum process condition should be A
3B
3C
1, namely the calcium hydrogen phosphate use amount 35%, micropowder silica gel use amount 1.5%, and concentration of alcohol is for being 5O%.
Claims (9)
1. a tiger is joined the relieving cough and asthma composition of medicine, and it is characterized in that: the active component of composition of medicine is comprised of the extract of Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae.
2. tiger according to claim 1 is joined the relieving cough and asthma composition of medicine, and it is characterized in that: the weight ratio of described Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae is 4-12:10-16:4-9:2-6.
3. tiger according to claim 2 is joined the relieving cough and asthma composition of medicine, and it is characterized in that: the weight ratio of described Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae is 17:26:13:9.
4. the described tiger ginseng of claim 1-3 relieving cough and asthma composition of medicine is applied in preparing antitussive medicine.
5. the described tiger ginseng of claim 1-3 relieving cough and asthma composition of medicine is applied in preparing suppressing panting calming medicine.
6. the described tiger ginseng of claim 1-3 relieving cough and asthma composition of medicine is applied in preparing expelling phlegm drugs.
7. the described tiger ginseng of claim 1-3 relieving cough and asthma composition of medicine is applied in preparing the functions of detumescence, relieving inflammation medicine.
8. the described tiger ginseng of claim 1-3 relieving cough and asthma composition of medicine is applied in preparing the enhancing immunity medicine.
9. the preparation of a tiger ginseng relieving cough and asthma composition of medicine, is characterized in that: the preparation of the described tiger ginseng of claim 1-3 relieving cough and asthma composition of medicine is become to capsule.
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