CN103393768B - Rhizoma Polygoni Cuspidati and Adenophora stricta combined medicine for cough relieving and panting calming, and preparation and application thereof - Google Patents
Rhizoma Polygoni Cuspidati and Adenophora stricta combined medicine for cough relieving and panting calming, and preparation and application thereof Download PDFInfo
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Abstract
The invention provides a Rhizoma Polygoni Cuspidati and Adenophora stricta combined medicine for cough relieving and panting calming. The aqueous extract of the combined medicine has a good activity in the cough relieving, panting calming, phlegm eliminating, swelling subduing, inflammation prevention and the like, and the 70% alcohol extract of the combined medicine has a good activity in immunity enhancement.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation, relate to a kind of tiger ginseng relieving cough and asthma composition of medicine, preparation and application thereof in particular.
Technical background
Traditional medicine is thought, chronic bronchitis belong in exopathogen more invade, caused by endogenous cough, and eating and drinking without temperance, dysfunction of the spleen in transportation, raw wet poly-expectorant; Internal organs imbalance, lung defends deficiency, causes expectorant to lose resistance lung, shows as the diseases such as cough, expectoration, asthma, control should detoxicating, relieving inflammation, relieving cough and resolving phlegm, relieving asthma.Modern medicine is thought, chronic bronchitis has infection, chemical factors, climate change, intolerance factors etc. all can cause chronic bronchitis more.Treatment often adopts infection control, eliminating phlegm and relieving cough, spasmolytic to relieving asthma, and no matter be traditional medicine or modern medicine, the treatment of this disease has obvious concordance.
Summary of the invention
For solving the problem, the invention provides a kind of tiger ginseng relieving cough and asthma composition of medicine, preparation and application thereof; Traditional medicine thinks that the cause of disease of chronic cough, chronic bronchitis, tracheitis and pathogenesis are: diseases caused by exogenous pathogenic factor pathogen, caused by endogenous cough, as eating and drinking without temperance, dysfunction of the spleen in transportation, and raw wet poly-expectorant; Internal organs imbalance, lung defends deficiency, and the room of causing rises and respectfully falls unbalance, causes expectorant to lose and hinders lung, show as the diseases such as cough, expectoration, asthma, control should detoxicating, relieving inflammation, relieving cough and resolving phlegm, relieving asthma, to take stopgap measures.
Consider chronic tracheitis, bronchitis be mostly repeatedly exopathogen invade caused by, therefore side in based on detoxicating, relieving inflammation; In side, Rhizoma Polygoni Cuspidati, Fructus Cnidii are detoxicating, relieving inflammation key medicine, and modern pharmacological research shows, Rhizoma Polygoni Cuspidati, Fructus Cnidii all have significantly antibacterial and antiinflammatory action.Radix Adenophorae is cough-relieving key medicine, main containing β ?Sitosterols material, tool antitussive effect, and Radix Glycyrrhizae is tool removing toxic substances and coordinating the actions of various ingredients in a prescription effect, the obvious antiinflammation of tool and the effect of corticoid sample also.
Invades in we's card many companions exopathogen, for a long time then endogenous cough, and then cause lung to defend deficiency, expectorant erosion resistance lung.Common deficiency syndrome, are mingled with excess pathogen.In side, Rhizoma Polygoni Cuspidati is bitter, sour, cool, clearing away heat-damp and promoting diuresis, and promoting blood circulation and detoxication, to effect a permanent cure, is monarch drug; Fructus Cnidii acrid, bitter, warm, warming the kidney to activate YANG, removing dampness and killing parasites, helps Rhizoma Polygoni Cuspidati detoxification and promoting urination, thinks ministerial drug; Radix Adenophorae is sweet, cool, lung moistening, eliminate the phlegm, cough-relieving to take stopgap measures, be adjuvant drug; Radix Glycyrrhizae is sweet, flat, spleen invigorating eliminating dampness by diuresis, and expelling phlegm for arresting cough, removing toxic substances, help the monarch and his subjects' eliminating damp, detoxifying, closes Radix Adenophorae and shares, then expelling phlegm for arresting cough, coordinating the actions of various ingredients in a prescription effect of holding concurrently, therefore for making.All medicines share, then tool removing toxic substances, antiinflammatory, dampness removing, cough-relieving, the effect of eliminating the phlegm, relievining asthma, treating both the principal and secondary aspects of a disease, all card spontaneous recovery.
The crude drug adopted in we: Radix Adenophorae is traditional cough-relieving key medicine, and Radix Glycyrrhizae also often uses; Rhizoma Polygoni Cuspidati tool clearing away heat-damp and promoting diuresis, controls blood detoxicating functions, is traditional detoxicating, relieving inflammation medicine; Fructus Cnidii tool warming the kidney to activate YANG, removing dampness and killing parasites effect.Modern pharmacological research shows, in Radix Adenophorae β ?the obvious antitussive action of sitosterol tool; The effect of Radix Glycyrrhizae tool class cortical hormone sample, namely has an antiinflammation; The obvious antibacterial action of Rhizoma Polygoni Cuspidati tool; Fructus Cnidii then has parahormone sample function, has anti-allergic effects, has antibacterial action concurrently; Application of composite, then have detoxicating, relieving inflammation, a cough and asthma relieving efficacy.Traditional treatment chronic bronchitis, chronic tracheitis, adopt palliative more, namely based on relieving cough and asthma, eliminate the phlegm, accompany by spleen invigorating eliminating dampness by diuresis, the medicines such as warming the kidney to promote diuresis are to solve expectorant erosion resistance lung problem, less employing detoxicating, relieving inflammation Drug therapy simultaneously.
This Therapeutic Method then adopts based on detoxicating, relieving inflammation, and double relieving cough and asthma of controlling eliminates the phlegm.Namely this Therapeutic Method principle meets Traditional Chinese medical theory, also matches with modern medical theory, and chronic tracheitis, bronchitis are many with infection (namely invading in exopathogen), and are paathogenic factor.Therefore, this prescription has good novelty, and traditional medicine and modern medical theory all support its feasibility simultaneously.
A kind of tiger ginseng relieving cough and asthma composition of medicine, the active component of composition of medicine is made up of the extract of Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae.
The weight ratio of described Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae is 4-12:10-16:4-9:2-6.
The weight ratio of described Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae is 17:26:13:9.
Described tiger ginseng relieving cough and asthma composition of medicine is applied preparing in antitussive medicine.
Described tiger ginseng relieving cough and asthma composition of medicine is applied preparing in suppressing panting calming medicine.
Described tiger ginseng relieving cough and asthma composition of medicine is applied preparing in expelling phlegm drugs.
Described tiger ginseng relieving cough and asthma composition of medicine is applied preparing in functions of detumescence, relieving inflammation medicine.
Described tiger ginseng relieving cough and asthma composition of medicine is applied preparing in enhancing immunity medicine.
A preparation for tiger ginseng relieving cough and asthma composition of medicine, becomes capsule by the preparation of above-mentioned tiger ginseng relieving cough and asthma composition of medicine.
Advantageous Effects of the present invention is: the invention provides a kind of tiger ginseng relieving cough and asthma composition of medicine, the water extract of this composition of medicine in antitussive, relieving asthma, eliminate the phlegm, there is good activity in functions of detumescence, relieving inflammation etc.; 70% ethanol extract of this composition of medicine has good activity in enhancing immunity.
Detailed description of the invention
The preparation method of embodiment 1 tiger ginseng relieving cough and asthma composition of medicine
Take Rhizoma Polygoni Cuspidati 850g, Fructus Cnidii 1300g, Radix Adenophorae 650g, Radix Glycyrrhizae 450g; Merge above-mentioned medical material, pulverize, respectively with weight 5 times, 3 times and 2 times of water backflows, each 30min, merges aqueous solution, filters, is condensed into extractum, as tiger ginseng relieving cough and asthma group 1;
Take Rhizoma Polygoni Cuspidati 400g, Fructus Cnidii 1600g, Radix Adenophorae 900g, Radix Glycyrrhizae 200g; Merge above-mentioned medical material, pulverize, respectively with weight 5 times, 3 times and 2 times of water backflows, each 30min, merges aqueous solution, filters, is condensed into extractum, as tiger ginseng relieving cough and asthma group 2;
Take Rhizoma Polygoni Cuspidati 1200g, Fructus Cnidii 1000g, Radix Adenophorae 400g, Radix Glycyrrhizae 600g; Merge above-mentioned medical material, pulverize, respectively with weight 5 times, 3 times and 2 times of water backflows, each 30min, merges aqueous solution, filters, is condensed into extractum, as tiger ginseng relieving cough and asthma group 3;
Take Rhizoma Polygoni Cuspidati 850g, Fructus Cnidii 1300g, Radix Adenophorae 650g, Radix Glycyrrhizae 450g; Merge above-mentioned medical material, pulverize, infiltrate with 500mL70% ethanol, use 5000mL70% ethanol percolation, percolation flow velocity is 2mLmin
-1, merge percolate, filter, be condensed into extractum, as tiger ginseng relieving cough and asthma group 4.
The antitussive effect of embodiment 2 tiger ginseng relieving cough and asthma composition of medicine is investigated
1. tiger ginseng relieving cough and asthma composition of medicine draws the impact coughed on mice ammonia
By the healthy mice of body weight 20 ± 2g, with the cough of ammonia Induced by Stimulation, in 3min, cough number of times reaches is for more than three times that screening is qualified.Get the mice that screening is qualified, be divided into six groups at random.Tiger ginseng relieving cough and asthma composition of medicine 5.5 crude drugs/kg that the other ig embodiment of drug component obtains, positive controls SC Codeine Hydrochloride 60mg/kg, matched group is ig equivalent distilled water then.Once a day, continuous three days (except Codeine Hydrochloride), last administration 1h(Codeine Hydrochloride 30min) after, undertaken by screening technique, statistics often organizes cough number of times in mice 3min, and result statistic software SPSS 10.0 one factor analysis of variance method is checked.Experimental result is as shown in table 1.
Table 1 tiger ginseng relieving cough and asthma composition of medicine draws the impact coughed on mice ammonia
With matched group than * P<0.05 * * P<0.01
2. tiger ginseng relieving cough and asthma composition of medicine causes the impact coughed on Cavia porcellus citric acid
Get body weight 200 ~ 250g Cavia porcellus, be placed in the airtight bell jar of 4L volume, spray into 52.5% citric acid with 400mmHg pressure, in spraying 1min, 5min, more than guinea pig cough number of times >=10 time person, qualified for screening.
The qualified Cavia porcellus of screening is divided into six groups (n=10) at random, drug component other ig tiger ginseng relieving cough and asthma composition of medicine 4.5 crude drugs/kg, positive controls SC Codeine Hydrochloride 40mg/kg, matched group ig equivalent distilled water.Be administered once.1h after medicine, tests by pre-selection method, record guinea pig cough's incubation period and 5min cough number of times (when cough does not appear in 5min, incubation period is 5min meter on schedule).Result statistic software SPSS 10.0 one factor analysis of variance method is checked.Result is as shown in table 2.
Table 2 tiger ginseng relieving cough and asthma composition of medicine causes the effect coughed to Cavia porcellus citric acid
With matched group than * P<0.05 * * P<0.01
Table 2 shows: tiger ginseng relieving cough and asthma composition of medicine obviously can resist the guinea pig cough that citric acid causes, and each dosage all can significant prolongation cough latent period (P<0.05-0.01).
Embodiment 3 tiger ginseng relieving cough and asthma composition of medicine antiasthmatic effect is investigated
1. tiger ginseng relieving cough and asthma composition of medicine causes the impact of Experimental Asthma In Guinea-pigs on histamine
Get about 200g Cavia porcellus, in the closed glass cover of 4L volume, spray into 2% acetylcholine and 0.4% histamine phosphate 2ml:4ml mixed liquor with 400mmHg constant voltage, spray 15S at every turn.In record 6min Cavia porcellus to occur that asthmatic tics is less than 150S person incubation period qualified for screening.
The qualified Cavia porcellus of screening is divided into six groups (n=10) at random, 4 groups of tiger ginseng relieving cough and asthma composition of medicine 4.5g crude drug/kg that the other ig embodiment 1 of drug component obtains, positive controls ip aminophylline 0.1g/kg, matched group ig equivalent distilled water.Be administered once.1h(aminophylline 30min after medicine) after, test by screening technique.The incubation period (do not occur person in 6min, depending on be incubation period 6min) that in record 6min, animal is had a convulsion.Result statistic software SPSS 10.0 adds up each group of incubation period.Result of the test is as shown in table 3.
Table 3 tiger ginseng relieving cough and asthma composition of medicine draws the effect of breathing heavily to Guinea Pig Histamine
* P<0.05 * * P<0.01 is compared with matched group
Table 3 shows: tiger ginseng relieving cough and asthma composition of medicine group all obviously can extend histamine and cause the incubation period that Cavia porcellus twitches, and has significant protective effect (P<0.05) to panting of bringing out of histamine.
2. egg protein is caused to the impact of Cavia porcellus allergic asthma
Get about 200g Cavia porcellus, inject 4% egg protein normal saline 0.1ml(side prior to thigh lateral muscle meat), every guinea pig intraperitoneal injection absorption DTP vaccine 1ml sensitized animal simultaneously.Animal was divided into six groups at random in second day, medicine group ig tiger ginseng relieving cough and asthma composition of medicine 4.5 crude drugs/kg, positive controls ig ketotifen sheet 1mg/kg, matched group ig equivalent distilled water, every day 1 time, continuous 14 days.After last medicine 1 hour, carry out constant-pressure atomization 20s with 4% egg protein normal saline, then observe accelerated breathing, dyspnea appear in Cavia porcellus in 6min, tic is fallen and suffer a shock incubation period, cough number of times and shock animals number occurs.Result is respectively with the inspection of statistic software SPSS 10.0 one factor analysis of variance method.Result of the test is as shown in table 4.
Table 4 tiger ginseng relieving cough and asthma composition of medicine causes the impact of Cavia porcellus allergic asthma on egg protein
* P<0.05 is compared, * * P<0.01 with matched group
From table 4, tiger ginseng relieving cough and asthma composition of medicine causes Cavia porcellus allergic asthma to egg protein significant protective effect.Obviously can extend the incubation period of dyspnea that egg protein causes and shock, significantly to the guinea pig cough that anti-egg protein causes, reduce shock animals number (P<0.05-0.01).
Embodiment 3 tiger ginseng relieving cough and asthma composition of medicine phlegm-dispelling functions is investigated
1. tiger ginseng relieving cough and asthma composition of medicine is on the impact of rat capillary tube method expectoration amount
Choosing body weight is 200 ± 20g healthy rat, after pentobarbital sodium anesthesia, faces upward position and fixedly keeps flat, cut skin of neck, be separated trachea, insert a capillary glass tube, make juice be discharged into capillary tube at correct position.Juice column length in an every 30 minutes records capillary tube, after 2h, by the random administration of table 5 dosage, records juice column length in 2h capillary tube with method after medicine again.Statistical, with average secretory volume per hour before administration for normal value, after administration, average secretory volume per hour is as being 170% of normal value, thinks there is phlegm-dispelling functions.According to said method evaluate the expectorant effect of tiger ginseng relieving cough and asthma composition of medicine.Result of the test is as shown in table 5.
Table 5 tiger ginseng relieving cough and asthma composition of medicine is on the impact of rat capillary tube method expectoration amount
Note: * after representing medicine expectoration amount per hour be more than 170% before medicine
From table 5, compare with matched group, tiger ginseng relieving cough and asthma composition of medicine has significant phlegm-dispelling functions to rat.
2. on the impact that the phenol red method of mice is eliminated the phlegm
Get body weight 20 ± 2g healthy mice, ♀ ♂ half and half, be divided into six groups at random.Drug component other gavage tiger ginseng relieving cough and asthma composition of medicine 5.5g crude drug/kg, positive controls ig ammonium chloride 1.0g/kg, matched group ig equivalent distilled water.Once a day, for three days on end, put to death after 30min after last administration, every Mus ip0.6% phenol red 0.1ml/10g, ip30min, be separated trachea, with 38 DEG C of 5%NaHCO
3solution 2ml divides three flushing tracheas.Collect flushing liquor, with 72 type spectrophotometers in 546nm wavelength colorimetric, result statistic software SPSS 10.0 one factor analysis of variance method is checked.Result of the test is as shown in table 6.
The impact that table 6 tiger ginseng relieving cough and asthma composition of medicine eliminates the phlegm on the phenol red method of mice
* P<0.05 * * P<0.01 is compared with matched group
Table 6 shows: the big or middle dosage of tiger ginseng relieving cough and asthma composition of medicine significantly can promote the discharge that mice tracheal strips is phenol red, compared with the control, has obvious expectorant effect (P<0.05).
Embodiment 4 tiger ginseng relieving cough and asthma composition of medicine antiinflammatory action is investigated
1. tiger ginseng relieving cough and asthma composition of medicine swells on rat granuloma the impact of hypertrophy
Get body weight 200 ± 20g healthy rat 72, under ether light anaesthesia aseptic condition, make abdominal incision, at left and right groin subcutaneous each implantation 10mg through autoclaving, oven dry drip and have the cotton balls of 0.2ml penicillin.Post operation rat is divided into six groups at random, starts administration, medicine group ig tiger ginseng relieving cough and asthma composition of medicine 4.5g crude drug/kg, positive controls ig prednisone 25mg/kg, matched group ig equivalent distilled water, once a day, successive administration 10 days.Put to death rat after last administration 1h, peel off and take out cotton balls granulation tissue, oven dry is weighed, and deducts raw cotton ball weight, is granuloma net weight, and result statistic software SPSS 10.0 one factor analysis of variance method is checked.Respectively organize granuloma weight; Result of the test is as shown in table 7.
Table 7 tiger ginseng relieving cough and asthma composition of medicine swells to rat granuloma the inhibitory action of hypertrophy
With matched group than * P<0.05 * * P<0.05
Table 7 is visible: compare with matched group, and tiger ginseng relieving cough and asthma composition of medicine group has significant inhibitory action (P<0.05-0.01) to the rat granuloma hypertrophy that swells.
2. on the bronchitic impact of murine chronic
Get body weight 20 ± 2g healthy mice 80, wherein make normal control for 14.All the other mice moulding: mice is put in airtight carton, light two medicated cigarettes, take out and be placed in 2-8 DEG C of refrigerator, freezing 30min at once after sootiness, and every day at the upper and lower noon is each once.After 20 days, get minority moulding mice trachea, lung examines with normal mouse malingering and compare, after determining modeling success, moulding mice is divided into six groups at random: tiger ginseng relieving cough and asthma composition of medicine (the present embodiment is referred to as tiger ginseng 1,2,3,4) 5.5g crude drug/kg, positive controls ig dexamethasone 0.02g/kg that the other ig embodiment 1 of drug component prepares, model group and matched group equal ig equivalent distilled water.Every day is administered once, continuous 10 days, weighs Mouse Weight and puts to death, get lung and weigh after last administration 1h, checks by statistic software SPSS 10.0 one factor analysis of variance method.Get lung, trachea is fixed in 10% formalin, make pathology detection, main detection four indexs: chronic inflammation cellular infiltration, bronchial mucosa epithelial proliferation, bronchial mucosa necrosis come off, goblet cell increases.The each index of every Mus judged with " slightly " or " obviously ", and each indicator and model group compares, X 2 test.Result of the test is as shown in table 8,9.
Table 8 tiger ginseng relieving cough and asthma composition of medicine is on the impact of the heavy coefficient of Chronic Bronchitis Mice lung
* P<0.05 is compared with matched group
Table 9 tiger ginseng relieving cough and asthma composition of medicine is on the impact of Chronic Bronchitis Mice lung, bronchus pathological change
* P<0.05**P<0.01 is compared with model group; Compare with matched group
△ △p<0.01
(1) table 8 shows: compare with model group, and tiger ginseng relieving cough and asthma composition of medicine has no significant effect the heavy coefficient of Chronic Bronchitis Mice lung.
(2) table 9 is visible: model group compares with normal group, and each disease inspection index has significant difference.And tiger ginseng big or middle dose of group compares with model group, each sick inspection index of murine chronic bronchitis all has clear improvement, and especially increases mice inflammatory airways cellular infiltration, bronchial mucosa epithelial proliferation and goblet cell, has significant inhibitory action.Illustrate that tiger ginseng relieving cough and asthma composition of medicine has and significantly suppress the bronchitic effect of murine chronic.
3. tiger ginseng relieving cough and asthma composition of medicine xylol causes the impact of mice ear
Get body weight 20 ± 2g healthy mice 60, male and female half and half, be divided into six groups at random.Drug component other ig tiger ginseng relieving cough and asthma composition of medicine 5.5 crude drugs/kg, positive controls ig prednisone 5mg/kg, matched group ig equivalent distilled water.Once a day, continuous three days, after last administration 1h, only be coated with dimethylbenzene 20 μ L/ at every Mus auris dextra, putting to death after 20min, is 8mm with diameter, card punch takes off two auricles and weighs, and with the difference of two auricle weight for swelling, result statistic software SPSS 10.0 one factor analysis of variance method is checked.Shown in result of the test table 10.
Table 10 tiger ginseng relieving cough and asthma composition of medicine xylol causes the effect of mice ear
* P<0.05 is compared with matched group
From table 7, compared with matched group, tiger ginseng relieving cough and asthma composition of medicine xylol causes mice ear certain antagonism, but effect is not significantly (P>0.05).
4. tiger ginseng relieving cough and asthma composition of medicine is on the impact of mice capillary permeability
Get body weight 20 ± 2g mice, be divided into six groups at random, respectively ig tiger ginseng relieving cough and asthma composition of medicine 5.5g crude drug/kg, prednisone 5mg/kg, equivalent distilled water.Once a day, continuous three times.0.5% Yi Wensilan normal saline solution 0.1ml/10g body weight is injected in tail vein after last administration 1h, on the position skin of unhairing of mouse web portion center, melted paraxylene 0.03ml/ is only immediately, put to death after 20min, cut abdominal part Pigmented Skin, put into 7:3 acetone and mixed liquor of normal saline 4ml, soak 48h, centrifuging and taking supernatant, 610nm wavelength colorimetric.With the inspection of statistic software SPSS 10.0 one factor analysis of variance method, add up each group of optical density (OD) value.Experimental result is as shown in table 11.
Table 11 tiger ginseng relieving cough and asthma composition of medicine is on the impact of mice capillary permeability
With matched group than * P<0.05
Table 11 shows: compare with matched group, and the mice vascular permeability that tiger ginseng relieving cough and asthma composition of medicine xylol causes increases without obvious antagonism (P>0.05).
Embodiment 5 tiger ginseng relieving cough and asthma composition of medicine is on the impact of nonspecific immunity function
1. normal mouse reticuloendothelial system (RES) is engulfed to the impact of carbon clearance ability in blood flow
Get body weight 20 ± 2g healthy mice 60, ♀ ♂ half and half, be divided into six groups at random, drug component other ig tiger ginseng relieving cough and asthma composition of medicine 5.5 crude drugs/kg, positive controls ig levamisole 80mg/kg, matched group ig equivalent distilled water.Every day ig once, continuous 7 days, after last medicine 1 hour, every Mus tail iv india ink 0.1ml/10g, respectively got eye socket blood 20 μ l in 30s, 5min after iv, is placed in and 2ml0.5%NaHCO is housed
3in vitro, by 72 type spectrophotometer wavelength 670nm colorimetrics, read optical density, result statistic software SPSS 10.0 one factor analysis of variance method is checked.Result of the test is as shown in table 12.
Table 12 engulfs the impact of carbon clearance ability in blood to mice RES
* P<0.05 is compared with matched group
From table 12, compare with matched group, tiger ginseng relieving cough and asthma composition of medicine has certain effect to enhancing normal mouse RES carbon clearance ability in blood flow of engulfing, but DeGrain (P>0.05), do not cause the change of Mouse Liver, spleen weight.
2. tiger joins the impact that relieving cough and asthma composition of medicine generates normal mouse hemolytic antibody
Get body weight 20 ± 2g healthy mice 50, ♀ ♂ half and half, be divided into six groups at random.Each group of difference ig tiger ginseng relieving cough and asthma composition of medicine 5.5g crude drug/kg, levamisole 80mg/kg, equivalent distilled water.Every day ig once, continuous 10 days.Administration the 3rd day, every Mus ip4% chicken erythrocyte suspension 0.2ml, carries out immunity to animal.After last administration 1h, eye socket gets blood 20 μ l, adds in 1ml normal saline and mixes, then adds the mixed liquor of the chicken erythrocyte suspension of 0.5ml5%, 0.5ml normal saline and fresh guinea pig serum 1:15.37 DEG C of waters bath with thermostatic control 30 minutes are put into, stopped reaction in refrigerator after mixing, centrifugal, get supernatant 1ml and add 3ml Dou Shi liquid, leave standstill after 10 minutes, in 72 type spectrophotometer 540nm wavelength colorimetrics, measure optical density (OD), OD value × 1000 are serum hemolysin value.Result statistic software SPSS 10.0 one factor analysis of variance method is checked.Result of the test is as shown in table 13.
The impact that table 13 tiger ginseng relieving cough and asthma composition of medicine generates mice hemolytic antibody
* * P<0.01 is compared with matched group
Table 13 is visible: tiger ginseng relieving cough and asthma composition of medicine generates normal mouse hemolytic antibody certain promotion, but acts on not obvious (P>0.05).
The preparation of embodiment 6 tiger ginseng relieving cough and asthma composition of medicine
Supplementary material constitutive material: tiger ginseng relieving cough and asthma composition of medicine; Adjuvant: medical starch, calcium hydrogen phosphate, micropowder silica gel (additional) wetting agent: certain density alcoholic solution.
Tiger ginseng relieving cough and asthma composition of medicine granular mass criterion (see table 14).
Table 14 tiger ginseng relieving cough and asthma composition of medicine granular mass evaluation criterion (total score 100)
Tiger is joined relieving cough and asthma composition of medicine, medical starch, calcium hydrogen phosphate be placed in trough type mixing machine and mix, add certain density alcoholic solution and mix thoroughly as wetting agent in right amount and make suitable soft material, inspection soft material quality makes it to reach hands and pinches agglomerating, and namely faling apart of strandinging is advisable.With 30 order nylon sieve series wet granulars, within 55 DEG C, aeration-drying is about 1.5% to moisture, and with 2O mesh sieve granulate, dry granular adds micropowder silica gel, is always mixed even, fills after intermediate products passed examination with Autocapsulefillingmachine at " v " type mixer.
Orthogonal test and result: with tiger ginseng relieving cough and asthma composition of medicine granular mass evaluation criterion for inspection target, operate by the preparation of above-mentioned tiger ginseng relieving cough and asthma composition of medicine capsule, adopt orthogonal experiment, with calcium hydrogen phosphate use amount (A), micropowder silica gel use amount (B), concentration of alcohol (c) 3 factors, each factor is chosen 3 levels and is tested, and the factor level formulated selects L
9(4
3) orthogonal table arrange test, each factor fetch water put down in table 15, experimental result (see table 16) under Different factor level, variance analysis (see table 17).
Table 15 empirical factor water-glass
The L of table 16 preparation technology orthogonal experiment
9(3
4) table
Table 17 analysis of variance table
As can be seen from Table 17.Each factor affects size A>B>C respectively to tiger ginseng relieving cough and asthma composition of medicine capsule content uniformity.The impact on tiger ginseng relieving cough and asthma composition of medicine capsule content uniformity of calcium hydrogen phosphate use amount, micropowder silica gel use amount has significant, and optimum process condition should be A
3b
3c
1, i.e. calcium hydrogen phosphate use amount 35%, micropowder silica gel use amount 1.5%, concentration of alcohol is for being 5O%.
Claims (8)
1. a tiger ginseng relieving cough and asthma composition of medicine, is characterized in that: the active component of composition of medicine is made up of the extract of Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae;
The weight ratio of described Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae is 4-12:10-16:4-9:2-6.
2. tiger ginseng relieving cough and asthma composition of medicine according to claim 1, is characterized in that: the weight ratio of described Rhizoma Polygoni Cuspidati, Fructus Cnidii, Radix Adenophorae and Radix Glycyrrhizae is 17:26:13:9.
3. the tiger ginseng relieving cough and asthma composition of medicine that one of claim 1,2 is described is applied preparing in antitussive medicine.
4. the tiger ginseng relieving cough and asthma composition of medicine that one of claim 1,2 is described is applied preparing in suppressing panting calming medicine.
5. the tiger ginseng relieving cough and asthma composition of medicine that one of claim 1,2 is described is applied preparing in expelling phlegm drugs.
6. the tiger ginseng relieving cough and asthma composition of medicine that one of claim 1,2 is described is applied preparing in functions of detumescence, relieving inflammation medicine.
7. the tiger ginseng relieving cough and asthma composition of medicine that one of claim 1,2 is described is applied preparing in enhancing immunity medicine.
8. a preparation for tiger ginseng relieving cough and asthma composition of medicine, is characterized in that: the tiger ginseng relieving cough and asthma composition of medicine preparation described in claim 1 or 2 is become capsule.
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| CN201310216376.1A CN103393768B (en) | 2013-06-03 | 2013-06-03 | Rhizoma Polygoni Cuspidati and Adenophora stricta combined medicine for cough relieving and panting calming, and preparation and application thereof |
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| CN201310216376.1A CN103393768B (en) | 2013-06-03 | 2013-06-03 | Rhizoma Polygoni Cuspidati and Adenophora stricta combined medicine for cough relieving and panting calming, and preparation and application thereof |
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| CN103393768B true CN103393768B (en) | 2015-01-07 |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1541660A (en) * | 2003-04-29 | 2004-11-03 | 李茂盛 | Antipyretic antitussive |
| CN1559481A (en) * | 2004-03-04 | 2005-01-05 | 包头市千年健药物研究开发有限公司 | Chinese medicine compound formula preparation for treating asthma disease |
| CN1839992A (en) * | 2006-01-19 | 2006-10-04 | 胡建新 | Traditional Chinese medicine pill for clearing toxin of pneumonia |
| CN101411835A (en) * | 2007-10-18 | 2009-04-22 | 李国强 | Medicament for treating pneumonectasis and bronchitis and for relieving cough, calming panting, as well as resolving and dispelling phlegm |
| CN101549110A (en) * | 2008-04-01 | 2009-10-07 | 宋树恒 | Traditional Chinese medicine preparation for curing cough and asthma |
| CN101791292A (en) * | 2010-04-13 | 2010-08-04 | 江庆澜 | Application of polygonin atomizing agent as asthma-treating medicine |
| CN102626511A (en) * | 2012-03-31 | 2012-08-08 | 韩萍 | Drug composition used for relieving cough and eliminating phlegm |
| CN103083574A (en) * | 2013-01-21 | 2013-05-08 | 吴迎春 | Cough-relieving phlegm-expelling traditional Chinese medicine decoction |
-
2013
- 2013-06-03 CN CN201310216376.1A patent/CN103393768B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1541660A (en) * | 2003-04-29 | 2004-11-03 | 李茂盛 | Antipyretic antitussive |
| CN1559481A (en) * | 2004-03-04 | 2005-01-05 | 包头市千年健药物研究开发有限公司 | Chinese medicine compound formula preparation for treating asthma disease |
| CN1839992A (en) * | 2006-01-19 | 2006-10-04 | 胡建新 | Traditional Chinese medicine pill for clearing toxin of pneumonia |
| CN101411835A (en) * | 2007-10-18 | 2009-04-22 | 李国强 | Medicament for treating pneumonectasis and bronchitis and for relieving cough, calming panting, as well as resolving and dispelling phlegm |
| CN101549110A (en) * | 2008-04-01 | 2009-10-07 | 宋树恒 | Traditional Chinese medicine preparation for curing cough and asthma |
| CN101791292A (en) * | 2010-04-13 | 2010-08-04 | 江庆澜 | Application of polygonin atomizing agent as asthma-treating medicine |
| CN102626511A (en) * | 2012-03-31 | 2012-08-08 | 韩萍 | Drug composition used for relieving cough and eliminating phlegm |
| CN103083574A (en) * | 2013-01-21 | 2013-05-08 | 吴迎春 | Cough-relieving phlegm-expelling traditional Chinese medicine decoction |
Non-Patent Citations (3)
| Title |
|---|
| 张春梅等.蛇床子的药理研究进展.《实用药物与临床》.2006,第9卷(第1期),第55-57页. * |
| 虎杖药理作用研究进展;王霞等;《海军医学杂志》;20040630;第25卷(第2期);第179-181页 * |
| 魏巍等.南沙参的化学成分和药理作用研究进展.《药物评价研究》.2011,第34卷(第4期),第298-300页. * |
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