CN103392585B - New method for culturing kelp seedlings in scale without substratum - Google Patents

New method for culturing kelp seedlings in scale without substratum Download PDF

Info

Publication number
CN103392585B
CN103392585B CN201310296259.0A CN201310296259A CN103392585B CN 103392585 B CN103392585 B CN 103392585B CN 201310296259 A CN201310296259 A CN 201310296259A CN 103392585 B CN103392585 B CN 103392585B
Authority
CN
China
Prior art keywords
bulk kelp
kelp
sporoblast
culture
bulk
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201310296259.0A
Other languages
Chinese (zh)
Other versions
CN103392585A (en
Inventor
叶乃好
徐东
王依涛
张晓雯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Original Assignee
Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences filed Critical Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority to CN201310296259.0A priority Critical patent/CN103392585B/en
Publication of CN103392585A publication Critical patent/CN103392585A/en
Application granted granted Critical
Publication of CN103392585B publication Critical patent/CN103392585B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

Abstract

A new method for culturing kelp seedlings in scale without substratum includes steps of (1) obtaining kelp embryoid sporophore by means of gametophyte clone cross breeding, (2) moving mixed liquid containing the kelp embryoid sporophore into sterile PES culture media to prepare embryoid sporophore culture solution and breeding the kelp embryoid sporophore into kelp young sporophore of 200+/-20 micrometers, and (3) expanding a kelp young sporophore culture system gradually by a three-step culture method, increasing culture temperature, breeding the young sporophore into seedlings of 1-2cm gradually in culture containers by a ventilation suspension method. By the ventilation suspension method, the survival rate can be increased greatly and loss caused by rotten seedlings can be reduced. Due to the ventilation suspension method for culture of kelp, operation in kelp culture is simple, laborsaving and timesaving, limitation in mature main weed is avoided, the kelp seedlings can be cultured anytime according to actual requirements, and the survival rate and the culture density of unit water body are higher than those of conventional methods.

Description

Without adherance large-scale cultivation bulk kelp seedling new method
Technical field
The invention belongs to algae raising technology field, relate to particularly a kind of without adherance large-scale cultivation bulk kelp seedling new method.
Background technology
Bulk kelp [Macrocystis pyrifera (L.) Ag.] is under the jurisdiction of Phaeophyta (Phaeophyta), Laminariales (Laminariales), bulk kelp section (Lessoniaceae) in classification, Macrocystis (Macrocystis), being individual maximum in the world a kind of marine alga, is also the fastest Plants of growth rate in the world.Bulk kelp occupies very important status in natural ecosystems, is the multiple halobiontic habitats such as Bao, fish and other precious marine products, to maintaining the offshore ecological balance and preventing that large area animal diseases from playing a positive role; Meanwhile, bulk kelp has important using value, for the production of multiple chemical industry, medical product, is the important source material of producing algin and producing methane.
China is former to distribute without bulk kelp, the research of bulk kelp introduction and artificial fecundation, object is that Shi Qi China north Coast breeds, become the new marine alga Industrial Resources of China, after submarine forest to be formed, can be fish good habitat is provided, and the construction of the aquaculture of the China coast stock of fish and oceanic pasture is played an important role.The end of the seventies in last century, the U.S. once adopted umbrella stand type method under water to carry out bulk kelp culture experiment in the waters of growing without bulk kelp, failed to promote (Ryther1979/1980) because cost is too high.Neushul adopts sandbag method to carry out seabed sowing bulk kelp in the eighties subsequently, due to problems such as harmful animals, is still at present experimental stage.In recent years, along with fast development and the over-exploitation of the mankind to bulk kelp of abalone culture industry, existing bulk kelp resource can not meet the demand of abalone culture industry far away.Therefore, carry out bulk kelp seed rearing new method imperative.
The traditional seedling raising manners of bulk kelp is to adopt the method for trip Spore adhesion matrix to cultivate seedling, yet this seedling raising manners is subject to the maturation restriction that plants vegetables, and seedling raise period has limitation; Seed rearing needs, between specific seedling medium and seedling culturing vehicle, to need a large amount of human and material resources.In addition, due to the secondary attaching process of bulk kelp after existing rhizoid to come off, the de-phenomenon that seedling is serious, survival rate is on the low side of traditional seedling-cultivating method ubiquity seedling.
Summary of the invention
The technical problem to be solved in the present invention is to provide that a kind of the present invention can make full use of seedling culture space without adherance large-scale cultivation bulk kelp seedling new method, adopts ventilation suspension method to cultivate bulk kelp seedling and greatly improves survival rate, reduces the loss that de-seedling causes.
The present invention is achieved through the following technical solutions:
Without an adherance large-scale cultivation bulk kelp seedling new method, it comprises the steps:
(1) bulk kelp sporoblast body obtains: adopt the method for gametophyte clone crossbreeding (Novel method for breeding kelp seedlings with gametophyte cloning method 201110373578.8) to obtain bulk kelp sporoblast body;
(2) bulk kelp sporoblast body is grown: the mixed liquor that fills bulk kelp sporoblast body is moved in the PES medium of sterilizing, become sporoblast body culture fluid, bulk kelp sporoblast body grows that to grow up to be the bulk kelp juvenile sporophyte of 200 ± 20 μ m gradually;
(3) ventilation of bulk kelp juvenile sporophyte suspends and cultivates: by three step cultivations, expand gradually bulk kelp juvenile sporophyte cultivating system, improve cultivation temperature, adopt ventilation suspension method to cultivate and make bulk kelp juvenile sporophyte grow up to gradually 1-2cm seedling in culture vessel.
Further, the condition of culture that the described bulk kelp sporoblast body of step (2) is grown is intensity of illumination 10-20 μ mol photons m -2s -1, 10 ± 1 ℃ of temperature, light dark period 12:12h, cultivates 28-32 days with this understanding;
Further, in the incubation that the described bulk kelp sporoblast body of step (2) is grown, adopt siphon to change water law, every bottle is upgraded weekly half PES medium, manually shakes culture vessel 2～5 times every day simultaneously, and bulk kelp sporoblast body is suspended in PES medium.
Three described steps are cultivated:
The first step is cultivated: when bulk kelp sporoblast body length is developed to 200 ± 20 μ m juvenile sporophyte, add the sterilizing PES medium of 16-20 times of volume of sporoblast body culture fluid, at 20-30 μ mol photons m -2s -110 ± 1 ℃, under light dark period 12:12h condition, adopt ventilation suspension method to make the bulk kelp juvenile sporophyte of cultivation visible to naked eyes, be that bulk kelp juvenile sporophyte is wire, shank and blade are distinguished not obvious, in incubation, by adjustment, inflate intensity, bulk kelp juvenile sporophyte is evenly suspended in PES medium.
Further, during " first step cultivation ", adopt siphon to change water law and upgrade PES medium, upgrade weekly half PES medium.
Second step is cultivated: when the bulk kelp juvenile sporophyte length of blade over 60% reaches 0.4-0.5cm, the volume of medium is increased to 26-30 times of sporoblast body culture fluid volume, and every 5-6 days update all PES medium once;
The 3rd step is cultivated: when surpassing 60% bulk kelp juvenile sporophyte length, reach 1.0cm when above, the culture fluid volume in culture vessel is 34-38 times of sporoblast body culture fluid volume, and cultivation temperature is 12 ± 1 ℃; Within every 2～3 days, upgrade PES medium completely once, while changing water, thoroughly clear up culture vessel once.
In described PES medium, each chemical composition is as follows: sodium nitrate (NaNO 3) 46mg/L, glycerophosphate (C 3h 7na 2o 6p5H 2o) 6.66mg/L, boric acid (H 3bO 3) 3.8mg/L, the ethylenediamine tetra-acetic acid (C of 0.5M pH=8 10h 16n 2o 8) 18.2ml/L, Trismabase(C 4h 11nO 3) 66.6mg/L, six water ammonium ferric sulfate ((NH 4) 2fe (SO 4) 26H 2o) 2.34mg/L, iron chloride (FeCl 3) 100 μ g/L, manganese sulfate monohydrate (MnSO 4h 2o) 546 μ g/L, white vitriol (ZnSO 47H 2o) 73.4 μ g/L, cobalt sulfate (CoSO 47H 2o) 16 μ g/L, Cobastab 12(C 63h 88coN 14o 14p) 1.34 μ g/L, Cobastab 1(C 12h 16n 4oSHCl) 66 μ g/L, vitamin h (C 10h 16n 2o 3s) 0.66 μ g/L.
The present invention's beneficial effect compared with prior art
1) the present invention uses liquid nutrient medium completely in the seedling culture stage, compares traditional seedling curtain adherance seedling method, and the method more can make full use of seedling culture space; 2), due to the secondary attaching process of bulk kelp after existing rhizoid to come off, the present invention adopts ventilation suspension method to cultivate bulk kelp seedling can improve survival rate greatly, reduces the loss that de-seedling causes; 3) ventilation suspension method is cultivated bulk kelp seedling, not only simple to operate, time saving and energy saving, and the restriction that not planted vegetables by maturation, can cultivate at any time according to actual needs bulk kelp seedling, and survival rate and unit of water body are cultivated density and be far longer than conventional method.4) technical scheme of the present invention can be implemented on a large scale, meets the needs of bulk kelp seed cultivation.
Accompanying drawing explanation
The suspension method of ventilating under Fig. 1 laboratory condition is cultivated bulk kelp juvenile sporophyte
A: bulk kelp female and male gametophytes expands numerous, and incubation time is 2 months; B: bulk kelp female and male gametophytes mixes fertilization, and incubation time is 2 weeks; C: fertilized egg cell's division, engineer's scale-25 μ m; D: bulk kelp sporoblast body, is about 200 μ m, engineer's scale-25 μ m; E, F:10 ℃, in 8LPES cultivating system, ventilation suspends and cultivates, incubation time 1 month; G:12 ℃, in 10-12LPES cultivating system, ventilation suspends and cultivates, incubation time 1 month; H: bulk kelp juvenile sporophyte.Engineer's scale-1cm.
Embodiment
Below by embodiment, the technical solution of the present invention is further explained, but protection scope of the present invention is not subject to any pro forma restriction of embodiment.
Embodiment
This experiment was since on July 18th, 2012, to on December 20th, 2012, stop, ventilation suspension method is cultivated bulk kelp juvenile sporophyte result and is shown, the bulk kelp sporoblast body that adopts gametophyte clone breeding technique to obtain is then cultivated into the bulk kelp juvenile sporophyte seedling that is about 1-2cm under the controlled condition of laboratory.
(1) bulk kelp sporoblast body obtains: adopt the method for gametophyte clone crossbreeding (Novel method for breeding kelp seedlings with gametophyte cloning method 201110373578.8) to obtain sporoblast body.Bulk kelp female and male gametophytes is taken from Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science's germplasm resource bank.A large amount of numerous bulk kelp female and male gametophytes (Figure 1A) that expand during on September 20th, 1 2012 on July 18th, 2012.
(2) bulk kelp sporoblast body is grown: the mixed liquor (sporoblast body weight in wet base is 30mg) that 1ml is filled to bulk kelp sporoblast body moves in 500ml sterilizing conical flask, adds sterilizing PES medium to 450ml, becomes sporoblast body culture fluid.At 10-20 μ mol photons m- 2s- 1, 10 ± 1 ℃, 12h/12h(L/D) under condition, in 500ml conical flask, cultivate (Figure 1B), fertilized egg cell, through longitudinal, horizontal split (Fig. 1 C), develops into length approximately 200 μ m bulk kelps children spore daughters (Fig. 1 D) after 30 days; In incubation, adopt siphon to change water law, every bottle is upgraded weekly half PES medium, and every day while, manual shaking flask was 2～5 times, and bulk kelp sporoblast is suspended in medium.
(3) ventilation of bulk kelp juvenile sporophyte suspends and cultivates: by three step cultivations, expand gradually bulk kelp juvenile sporophyte cultivating system, improve cultivation temperature, adopt ventilation suspension method to cultivate bulk kelp juvenile sporophyte and grow up to gradually seedling in culture vessel.
The first step is cultivated: when bulk kelp sporoblast body length is developed to 200 μ m left and right bulk kelp juvenile sporophyte, 450ml sporoblast body culture fluid is placed in to 15L transparent plastic case, adds 8L sterilizing PES medium (Fig. 1 EF), at 20-30 μ mol photons m- 2s- 1, temperature is 10 ± 1 ℃, light dark period is 12:12h, inflation intensity (200～500ml min- 1) under condition, adopt ventilation suspension method to cultivate bulk kelp juvenile sporophyte visible to naked eyes, bulk kelp juvenile sporophyte is wire, and shank is not obvious with blade differentiation, in incubation, by adjustment, inflates intensity, and bulk kelp juvenile sporophyte is evenly suspended in medium.Adopt during this time siphon to change water law and upgrade PES medium, upgrade weekly half PES medium.
Second step is cultivated: when the juvenile sporophyte length of blade over 60% reaches 0.5cm, add the volume of PES medium to 12L, within every 5 days, upgrade PES medium once;
The 3rd step is cultivated: ventilation suspends and cultivates after 30 days, when surpassing the about 1.0cm(Fig. 1 of bulk kelp juvenile sporophyte length G of 60%), frond culture fluid volume is increased to 16L, and cultivation temperature is 12 ± 1 ℃; Within every 2～3 days, upgrade PES medium completely once, while upgrading PES medium, clear up culture vessel once.
Experimental session, synchronously adopts traditional adherance seedling method to cultivate bulk kelp seedling.To on December 20th, 2012, cultivation bulk kelp seedling experimental result was as shown in table 1.Bulk kelp seedling 3.2 ten thousand strains that ventilation suspension method is successfully cultivated average length 1.0cm, unit of water body seedling density reaches 25000ind/L, and survival rate reaches 95%.Wherein unit of water body seedling density is 13.3 times of traditional breeding method, and survival rate improves approximately 25%.
Two kinds of seedling raising mannerses of table 1 are cultivated bulk kelp seedling situation statistics
Seedling raising manners The strain number (ind) of emerging Seedling density (ind/L) Survival rate
Adherance seedling method 30000 150 75
Without adherance seedling method 320000 2000 90

Claims (4)

1. without an adherance large-scale cultivation bulk kelp seedling new method, it is characterized in that it comprises the steps:
(1) bulk kelp sporoblast body obtains: adopt the method for gametophyte clone crossbreeding to obtain bulk kelp sporoblast body;
(2) bulk kelp sporoblast body is grown: the mixed liquor that fills bulk kelp sporoblast body is moved in the PES medium of sterilizing, become sporoblast body culture fluid, bulk kelp sporoblast body grows that to grow up to be the bulk kelp juvenile sporophyte of 200 ± 20 μ m gradually;
(3) ventilation of bulk kelp juvenile sporophyte suspends and cultivates: by three step cultivations, expand gradually bulk kelp juvenile sporophyte cultivating system, improve cultivation temperature, adopt ventilation suspension method to cultivate and make bulk kelp juvenile sporophyte grow up to gradually 1-2cm seedling in culture vessel;
In described PES medium, each chemical composition is as follows: sodium nitrate (NaNO 3) 46mg/L, glycerophosphate (C 3h 7na 2o 6p5H 2o) 6.66mg/L, boric acid (H 3bO 3) 3.8mg/L, the ethylenediamine tetra-acetic acid (C of 0.5M pH=8 10h 16n 2o 8) 18.2ml/L, Trismabase (C 4h 11nO 3) 66.6mg/L, six water ammonium ferric sulfate ((NH 4) 2fe (SO 4) 26H 2o) 2.34mg/L, iron chloride (FeCl 3) 100 μ g/L, manganese sulfate monohydrate (MnSO 4h 2o) 546 μ g/L, white vitriol (ZnSO 47H 2o) 73.4 μ g/L, cobalt sulfate (CoSO 47H 2o) 16 μ g/L, Cobastab 12(C 63h 88coN 14o 14p) 1.34 μ g/L, vitamin B1 (C 12h 16n 4oSHCl) 66 μ g/L, vitamin h (C 10h 16n 2o 3s) 0.66 μ g/L;
Three described steps are cultivated:
The first step is cultivated: when bulk kelp sporoblast body length is developed to 200 ± 20 μ m juvenile sporophyte, add the sterilizing PES medium of 16-20 times of volume of sporoblast body culture fluid, at 20-30 μ mol photons m -2s -110 ± 1 ℃, under light dark period 12:12h condition, adopt ventilation suspension method to make the bulk kelp juvenile sporophyte of cultivation visible to naked eyes, be that bulk kelp juvenile sporophyte is wire, shank and blade are distinguished not obvious, in incubation, by adjustment, inflate intensity, bulk kelp juvenile sporophyte is evenly suspended in PES medium;
Second step is cultivated: when the bulk kelp juvenile sporophyte length of blade over 60% reaches 0.4-0.5cm, the volume of medium is increased to 26-30 times of sporoblast body culture fluid volume, and every 5-6 days update all PES medium once;
The 3rd step is cultivated: when surpassing 60% bulk kelp juvenile sporophyte length, reach 1.0cm when above, the culture fluid volume in culture vessel is 34-38 times of sporoblast body culture fluid volume, and cultivation temperature is 12 ± 1 ℃; Within every 2～3 days, upgrade PES medium completely once, while changing water, thoroughly clear up culture vessel once.
2. according to claim 1 a kind of without adherance large-scale cultivation bulk kelp seedling new method, it is characterized in that the condition of culture that the described bulk kelp sporoblast body of step (2) is grown is intensity of illumination 10-20 μ mol photons m -2s -1, 10 ± 1 ℃ of temperature, light dark period 12:12h, cultivates 28-32 days with this understanding.
3. according to claim 1 a kind of without adherance large-scale cultivation bulk kelp seedling new method; it is characterized in that adopting siphon to change water law in incubation that the described bulk kelp sporoblast body of step (2) grows; every bottle is upgraded weekly half PES medium; manually shake culture vessel 2～5 times every day simultaneously, bulk kelp sporoblast body is suspended in PES medium.
4. according to claim 1 a kind of without adherance large-scale cultivation bulk kelp seedling new method, it is characterized in that adopting siphon to change water law during " first step cultivation " and upgrade PES medium, upgrade weekly half PES medium.
CN201310296259.0A 2013-07-15 2013-07-15 New method for culturing kelp seedlings in scale without substratum Expired - Fee Related CN103392585B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310296259.0A CN103392585B (en) 2013-07-15 2013-07-15 New method for culturing kelp seedlings in scale without substratum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310296259.0A CN103392585B (en) 2013-07-15 2013-07-15 New method for culturing kelp seedlings in scale without substratum

Publications (2)

Publication Number Publication Date
CN103392585A CN103392585A (en) 2013-11-20
CN103392585B true CN103392585B (en) 2014-08-20

Family

ID=49556500

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310296259.0A Expired - Fee Related CN103392585B (en) 2013-07-15 2013-07-15 New method for culturing kelp seedlings in scale without substratum

Country Status (1)

Country Link
CN (1) CN103392585B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103704121A (en) * 2013-12-02 2014-04-09 中国海洋大学 Costaria costata gametophyte clone hybridization seedling raising technology
CN105766610B (en) * 2014-12-25 2019-05-14 朱文荣 A kind of seaweed spore aggregation method for culturing seedlings
CN104782470B (en) * 2015-04-01 2017-01-18 莆田市平海僧帽牡蛎原种场 Cultivating method for bangia
CN106106119A (en) * 2016-07-11 2016-11-16 象山旭文海藻开发有限公司 A kind of cultural method of Monostroma nitidum Wittr. spore collection massing

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5561736A (en) * 1978-10-28 1980-05-09 Nippon Carbide Kogyo Kk Laver breeding method and material
GB8707216D0 (en) * 1987-03-26 1987-04-29 Paisley College Of Technology Tissue culture
WO2002017707A1 (en) * 2000-08-31 2002-03-07 Council Of Scientific And Industrial Research An improved process for cultivation of algae
CN100494356C (en) * 2005-05-20 2009-06-03 中国科学院海洋研究所 Method for breeding energy alga-kelp sporophyte using gametophyte clone method
CN101422125B (en) * 2007-10-29 2011-04-06 中国水产科学研究院黄海水产研究所 Sargassum thunbergii seedling quick-propagation method using leader branch segment tillering method
CN101558793A (en) * 2008-04-17 2009-10-21 滕国新 Preparation technology for producing lactobacillus starter culture by cheese
CN102487820B (en) * 2011-11-22 2013-07-10 中国水产科学研究院黄海水产研究所 Novel method for breeding kelp seedlings with gametophyte cloning method
CN103098695B (en) * 2013-02-22 2014-08-06 中国水产科学研究院黄海水产研究所 Cultivation method of zostera marina high-quality seedling plants

Also Published As

Publication number Publication date
CN103392585A (en) 2013-11-20

Similar Documents

Publication Publication Date Title
CN105284620B (en) A kind of method that Superearly peach bybrid embryo saves seedling
CN107148928B (en) Method for cultivating hybrid variety of patinopecten yessoensis and wind vane scallop
CN103392585B (en) New method for culturing kelp seedlings in scale without substratum
CN102428870A (en) Preparation method for artificial seeds of dendrobium candidum
CN102577976A (en) Simple tissue culture method for broussonetia papyrifera
CN107135950A (en) A kind of breeding method of quick acquisition black fruit fructus lycii regrowth
CN103202214A (en) Breeding method of sargassum vachellianum artificial seeds
TW201517787A (en) Tissue culturing method, culturing method of ferns and explant obtained therefrom
CN103444603A (en) Method for culturing grouper fries in small water body
CN104094832B (en) Method for crossbreeding of undaria pinnatifida by using unisexual planospores
CN104304031A (en) Aquaculture rapid propagation method for potato virus-free tissue culture plantlets
CN104996298A (en) Method for cultivating polygala fallax hemsl tissue culture seedlings based on multiple internodal stem segments integration
CN105103714A (en) Manual germination accelerating method and seedling raising method of Bletilla sfriata (Thunb.)Reiehb.f.
CN103766218A (en) Butterfly orchid induction culture medium and asexual propagation method of butterfly orchid
CN105028215A (en) Rooting medium for tissue culture of bletilla striata and tissue culture method of bletilla striata
CN101984043A (en) Open cultivation method of diatom
CN103875527B (en) The artificial seed preparation method of a kind of submerged plant eel grass
CN110226510A (en) A kind of miscellaneous algae suppressing method in reef film category algae artificial culture
CN104285819B (en) The tissue culture propagation method of a kind of the southern regions of the Yunnan Province cuckoo
CN106172008A (en) A kind of Bowring cattleya fast culture propagation method
CN103651141A (en) Factory-like rapid propagation method for test-tube seedlings of dendranthema morifolium
CN202873417U (en) Novel type seedling cultivating nutrition bowl
CN104094854A (en) In-vivo ornamental plant in closed transparent container, and manufacturing method thereof
CN103283654B (en) Method for increasing settlement and metamorphosis rate in Apostichopus japonicus larvae
CN104041405B (en) A kind of method of rapid screening high yield sea-tangle strain

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140820

Termination date: 20150715

EXPY Termination of patent right or utility model