CN103389235A - Extracting method of acrylamide test sample in roasted food - Google Patents
Extracting method of acrylamide test sample in roasted food Download PDFInfo
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- CN103389235A CN103389235A CN2013103236435A CN201310323643A CN103389235A CN 103389235 A CN103389235 A CN 103389235A CN 2013103236435 A CN2013103236435 A CN 2013103236435A CN 201310323643 A CN201310323643 A CN 201310323643A CN 103389235 A CN103389235 A CN 103389235A
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Abstract
The invention relates to an extracting method of an acrylamide test sample in roasted food. The extracting method comprises the following steps of S1, weighing quantitative roasted food; S2, injecting normal hexane and an internal standard solution; S3, sufficiently mixing and discarding normal hexane phase; S4, adding water, heating in a water bath and ultrasonically extracting; S5, adding Carrez reagent I and Carrez reagent II, and uniformly mixing; S6, centrifuging in a centrifugal machine; S7, transferring centrifuged water phase, adding NaCl for ensuring that the water phase is saturated, and sufficiently and uniformly shaking; S8, extracting the water phase in S7 with ethyl acetate twice; S9, transferring the ethyl acetate phase; S10, adding anhydrous sodium sulfate to remove water; S11, evaporating and concentrating the dried organic phase in a rotary manner; and S12, filtering the sample to a small sample inlet bottle for being sampled and analyzed. The extracting method of the acrylamide test sample in the roasted food can be used for conveniently and quickly extracting the acrylamide sample, so that the subsequent acrylamide detection is conveniently carried out and is beneficial to supervising the safety problem of the roasted food.
Description
Technical field
The present invention relates to a kind of sample extraction method in food security detection industry, especially relate to the extracting method of acrylamide test sample in a kind of baked goods.
Background technology
fried at some and toast starch food products, as chips, detect acrylamide in chrisps etc., and content surpasses more than 500 times that allows maximum to limit the quantity of in drinking-water, research shows, human body can pass through alimentary canal, respiratory tract, the number of ways such as skin and mucosa contact acrylamide, acrylamide enters in body and can be absorbed by the body by number of ways again, wherein through alimentary canal, absorb the fastest, enter acrylamide in human body approximately 90% by metabolism, only discharge through urine with original shape on a small quantity, after acrylamide enters in body, guanine on DNA is combined and is formed adduct in vivo, cause damage of genetic materials and gene mutation, so acrylamide is very large to the harm of human body, the effect of supervision has been played in the detection of acrylamide to reducing the human injury.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of extracting method that can extract very easily acrylamide test sample in baked goods.
The present invention solves the technical scheme that its technical matters takes: the extracting method of acrylamide test sample in a kind of baked goods, and the step of this extracting method is:
S1: take quantitative baked goods, be placed in sample hose;
S2: inject normal hexane and interior mark liquation in sample hose;
S3: fully mix, discard the normal hexane phase;
S4: add water in sample hose, heating water bath and ultrasound wave extract sample afterwards;
S5: add the auspicious reagent I of card and Ka Rui reagent II and mix in sample hose;
S6: sample hose is centrifugal in hydro-extractor;
S7: the water in the sample hose after centrifugal is transferred to separating funnel, adds NaCl to make water saturated, fully shake up;
S8: with the water in twice extraction S7 of ethyl acetate;
S9: with the ethyl acetate phase transfer in conical flask;
S10: add anhydrous sodium sulfate to dewater in conical flask;
S11: dried organic phase is transferred to pear shape bottle, and rotary evaporation is concentrated;
S12: sample filtering, to the sample introduction bottle, is treated the sample introduction analysis.
Further concrete, in described step S4, the temperature of water-bath is at 60 ℃, and ultrasound wave extraction time is 30 minutes.
Further concrete, in described step S6, centrifugation time is 10 minutes.
The invention has the beneficial effects as follows: after having adopted said method, can facilitate and extract fast the acrylamide sample, guarantee to extract the reliability of sample, conveniently carry out the detection of follow-up acrylamide, be conducive to supervise the safety problem of baked goods.
Embodiment
The present invention is described in detail below in conjunction with embodiment.
The extracting method of acrylamide test sample in a kind of baked goods, the step of this extracting method is:
S1: take bread or other baked goods of 2g, be placed in the sample hose of 50ml;
S2: the interior mark liquation (10mg/L) that injects 10ml normal hexane and 25 μ l in sample hose;
S3: fully mix, discard the normal hexane phase;
S4: add the water of 25ml in sample hose, in the water-bath of 60 ℃, heating and ultrasound wave extract sample afterwards;
S5: add the auspicious reagent II of card of the auspicious reagent I of card of 2ml and 2ml and mix in sample hose;
S6: with sample hose in hydro-extractor centrifugal 10 minutes;
S7: the water in the sample hose after centrifugal is transferred to the separating funnel of 100ml, adds NaCl to make water saturated, fully shake up;
S8: with the water in twice extraction S7 of ethyl acetate of 25ml;
S9: the ethyl acetate phase transfer of above-mentioned 50ml is arrived in the conical flask of 100ml;
S10: add the 10g anhydrous sodium sulfate to dewater in conical flask;
S11: dried organic phase is transferred to pear shape bottle, and rotary evaporation is concentrated into 1ml;
S12: sample 0.45 μ m filtering membrane is filtered to the sample introduction bottle, treats the sample introduction analysis.
The sample that obtains is delivered in instrument and is carried out liquid chromatography mass and measure, according to survey situation judge whether the content of the acrylamide in baked goods exceeds standard, and with this, supervises the security of baked goods, guarantee that the eater's is healthy.
It is emphasized that: above is only preferred embodiment of the present invention, not the present invention is done any pro forma restriction, every foundation technical spirit of the present invention, to any simple modification, equivalent variations and modification that above embodiment does, all still belongs in the scope of technical solution of the present invention.
Claims (3)
1. the extracting method of acrylamide test sample in a baked goods, is characterized in that, the step of this extracting method is:
S1: take quantitative baked goods, be placed in sample hose;
S2: inject normal hexane and interior mark liquation in sample hose;
S3: fully mix, discard the normal hexane phase;
S4: add water in sample hose, heating water bath and ultrasound wave extract sample afterwards;
S5: add the auspicious reagent I of card and Ka Rui reagent II and mix in sample hose;
S6: sample hose is centrifugal in hydro-extractor;
S7: the water in the sample hose after centrifugal is transferred to separating funnel, adds NaCl to make water saturated, fully shake up;
S8: with the water in twice extraction S7 of ethyl acetate;
S9: with the ethyl acetate phase transfer in conical flask;
S10: add anhydrous sodium sulfate to dewater in conical flask;
S11: dried organic phase is transferred to pear shape bottle, and rotary evaporation is concentrated;
S12: sample filtering, to the sample introduction bottle, is treated the sample introduction analysis.
2. the extracting method of acrylamide test sample in a kind of baked goods according to claim 1, is characterized in that, in described step S4, the temperature of water-bath is at 60 ℃, and ultrasound wave extraction time is 30 minutes.
3. the extracting method of acrylamide test sample in a kind of baked goods according to claim 1, is characterized in that, in described step S6, centrifugation time is 10 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103236435A CN103389235A (en) | 2013-07-30 | 2013-07-30 | Extracting method of acrylamide test sample in roasted food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103236435A CN103389235A (en) | 2013-07-30 | 2013-07-30 | Extracting method of acrylamide test sample in roasted food |
Publications (1)
| Publication Number | Publication Date |
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| CN103389235A true CN103389235A (en) | 2013-11-13 |
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| CN2013103236435A Pending CN103389235A (en) | 2013-07-30 | 2013-07-30 | Extracting method of acrylamide test sample in roasted food |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1713824A (en) * | 2002-11-22 | 2005-12-28 | 宝洁公司 | Method for reducing acrylamide in foods, foods having reduced levels of acrylamide |
| CN101303304A (en) * | 2008-07-09 | 2008-11-12 | 厦门大学 | Method for testing acrylic amide in food |
| CN102507880A (en) * | 2011-11-08 | 2012-06-20 | 无锡安迪生物工程有限公司 | Card for quickly detecting acrylamide, and detection method therefor |
| CN102621079A (en) * | 2012-03-19 | 2012-08-01 | 齐齐哈尔大学 | Method and device for detecting acrylamide content in barbecued meat product on line |
-
2013
- 2013-07-30 CN CN2013103236435A patent/CN103389235A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1713824A (en) * | 2002-11-22 | 2005-12-28 | 宝洁公司 | Method for reducing acrylamide in foods, foods having reduced levels of acrylamide |
| CN101303304A (en) * | 2008-07-09 | 2008-11-12 | 厦门大学 | Method for testing acrylic amide in food |
| CN102507880A (en) * | 2011-11-08 | 2012-06-20 | 无锡安迪生物工程有限公司 | Card for quickly detecting acrylamide, and detection method therefor |
| CN102621079A (en) * | 2012-03-19 | 2012-08-01 | 齐齐哈尔大学 | Method and device for detecting acrylamide content in barbecued meat product on line |
Non-Patent Citations (2)
| Title |
|---|
| 呼德等: "焙烤小麦胚芽中丙烯酰胺含量的测定与分析", 《北京工商大学学报（自然科学版）》 * |
| 杨斯超等: "柱前衍生化-气相色谱-质谱法定量测定食品中丙烯酰胺的含量", 《色谱》 * |
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Application publication date: 20131113 |