CN103355141A - Method for improving provisonal planting survival rate of tissue culture rooting sugarcane seedlings - Google Patents
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- 240000000111 Saccharum officinarum Species 0.000 title claims abstract description 35
- 235000007201 Saccharum officinarum Nutrition 0.000 title claims abstract description 35
- 230000004083 survival effect Effects 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000000725 suspension Substances 0.000 claims abstract description 13
- 239000007921 spray Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 2
- 239000005784 Fluoxastrobin Substances 0.000 claims 4
- UFEODZBUAFNAEU-NLRVBDNBSA-N fluoxastrobin Chemical compound C=1C=CC=C(OC=2C(=C(OC=3C(=CC=CC=3)Cl)N=CN=2)F)C=1C(=N/OC)\C1=NOCCO1 UFEODZBUAFNAEU-NLRVBDNBSA-N 0.000 claims 4
- 239000005730 Azoxystrobin Substances 0.000 abstract description 10
- WFDXOXNFNRHQEC-GHRIWEEISA-N azoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1OC1=CC(OC=2C(=CC=CC=2)C#N)=NC=N1 WFDXOXNFNRHQEC-GHRIWEEISA-N 0.000 abstract description 10
- 241000196324 Embryophyta Species 0.000 abstract description 8
- 208000035240 Disease Resistance Diseases 0.000 abstract description 3
- 244000052616 bacterial pathogen Species 0.000 abstract description 3
- 244000052769 pathogen Species 0.000 abstract description 2
- 230000003449 preventive effect Effects 0.000 abstract description 2
- 230000001225 therapeutic effect Effects 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000002689 soil Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000004576 sand Substances 0.000 description 3
- 241000670727 Amida Species 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000033458 reproduction Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 230000011681 asexual reproduction Effects 0.000 description 1
- 238000013465 asexual reproduction Methods 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
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- Y02P60/216—
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Abstract
一种提高甘蔗组培生根苗假植成活率的方法，采用25%嘧菌酯悬浮液1000-1500倍对甘蔗组培生根假植苗进行处理，抑制病菌对组培苗的危害。本发明利用嘧菌酯对病菌强大的预防兼治疗作用，增强蔗苗的抗病能力，从而提高其成活率。使用方法简单，投资少，实用性强，组培生根苗成活率高，在低温潮湿梅雨季节假植成活率可达90％左右，其他季节假植成活率可达97％以上。A method for improving the survival rate of sugarcane tissue-cultured rooted plantlets. The sugarcane tissue-cultured rooted plantlets are treated with 1000-1500 times of 25% azoxystrobin suspension to inhibit the damage of pathogens to the tissue-cultured plantlets. The invention utilizes the strong preventive and therapeutic effects of azoxystrobin on pathogenic bacteria to enhance the disease resistance of sugarcane seedlings, thereby increasing their survival rate. The use method is simple, the investment is small, the practicability is strong, and the survival rate of rooted seedlings by tissue culture is high. The survival rate of false plants in the low-temperature and humid plum rain season can reach about 90%, and the survival rate of false plants in other seasons can reach more than 97%.
Description
技术领域technical field
本发明属于作物栽培病害防治领域，涉及一种提高甘蔗组培生根苗假植成活率的方法。The invention belongs to the field of crop cultivation disease prevention and control, and relates to a method for improving the survival rate of sugarcane tissue culture rooted seedlings.
背景技术Background technique
甘蔗是我国的主要糖料作物，种植面积占我国糖料作物总面积90％左右，蔗糖产量占我国食糖产量90％以上。甘蔗种苗生产传统上采用无性繁殖进行，繁殖系数低，对于新品种的快速推广不利，应用植物组培快繁技术繁育甘蔗良种，其繁殖系数比传统可提高1000倍以上；此外，甘蔗在生产过程中，受到多种病源物的反复侵染，种植多年后种茎积累较多的病源菌，抑制甘蔗生长，造成甘蔗产量和品质的大幅度下降，但积累在种茎上的部分病源菌应用化学药物无法去除，只有通过茎尖分生组织生产健康种苗去除，恢复良种的优良种性，提高甘蔗产量和糖分。Sugarcane is the main sugar crop in my country, the planting area accounts for about 90% of the total area of sugar crops in my country, and the sucrose output accounts for more than 90% of the sugar output in my country. The production of sugarcane seedlings is traditionally carried out by asexual reproduction, which has a low reproduction coefficient, which is not conducive to the rapid promotion of new varieties. The application of plant tissue culture rapid propagation technology to breed sugarcane varieties can increase the reproduction coefficient by more than 1000 times compared with traditional methods; During the process, they were repeatedly infested by various pathogenic substances. After planting for many years, the seed stems accumulated more pathogenic bacteria, which inhibited the growth of sugarcane and caused a significant decline in the yield and quality of sugarcane. However, some pathogenic bacteria accumulated on the seed stems were used Chemical drugs can not be removed, only through the production of healthy seedlings through the shoot tip meristem to remove, restore the excellent seed quality of the improved species, and increase the yield and sugar content of sugarcane.
甘蔗组培快繁及健康种苗生产技术已大面积应用于生产，其室内培养技术已比较成熟。由于组培生根苗是在营养物质丰富、光照、温度、湿度等严格人工控制条件下培养出来的苗，比较弱小，需经假植培育后才能定植大田。组培生根苗从培养瓶或袋移栽到育苗圃，其生长环境从无菌、异养、生长因素相对稳定等的条件下转变为有菌、自养、生长环境因素变化不定，其抗逆能力低，在假植过程中因受各种因素的影响其成活率变化较大，高的可达90％左右，最低有时不到10％。因此，如何提高甘蔗组培苗假植成活率是我国甘蔗组培苗生产的主要关键技术问题之一。Sugarcane tissue culture rapid propagation and healthy seedling production technology has been widely used in production, and its indoor cultivation technology is relatively mature. Since the rooted seedlings of tissue culture are the seedlings cultivated under strict artificial control conditions such as rich nutrients, light, temperature, and humidity, they are relatively weak and need to be cultivated by artificial planting before they can be planted in the field. Tissue culture rooted seedlings are transplanted from culture bottles or bags to nurseries, and their growth environment changes from sterile, heterotrophic, and relatively stable growth factors to bacteria-free, autotrophic, and variable growth environmental factors. The ability is low, and the survival rate varies greatly due to the influence of various factors in the process of artificial planting, the highest can reach about 90%, and the lowest is sometimes less than 10%. Therefore, how to improve the survival rate of sugarcane tissue cultured plantlets is one of the main key technical issues of my country's sugarcane tissue cultured plantlets production.
发明内容Contents of the invention
本发明的目的在于提供一种提高甘蔗组培生根苗假植成活率的方法，能够增强蔗苗的抗病能力，从而提高其成活率。方法简单，投资少，实用性强，组培生根苗成活率高，在低温潮湿梅雨季节假植成活率可达90％左右，其他季节假植成活率可达97％以上。The object of the present invention is to provide a method for improving the survival rate of sugarcane tissue culture rooted seedlings, which can enhance the disease resistance of sugarcane seedlings, thereby improving their survival rate. The method is simple, low in investment, strong in practicability, and has a high survival rate of rooted seedlings through tissue culture. The survival rate of false plants in the low temperature and humid rainy season can reach about 90%, and the survival rate of false plants in other seasons can reach more than 97%.
本发明采用以下技术方案实现上述目的：一种提高甘蔗组培生根苗假植成活率的方法，包括如下步骤：采用25%嘧菌酯悬浮液对甘蔗组培假植苗进行处理。The present invention adopts the following technical solutions to achieve the above object: a method for improving the survival rate of sugarcane tissue-cultured rooted seedlings, comprising the following steps: using 25% azoxystrobin suspension to treat the sugarcane tissue-cultured false plants.
所述假植苗为组培生根苗栽植在育苗床上。The false seedlings are rooted seedlings planted on seedling beds.
所述采用25%嘧菌酯悬浮液对甘蔗组培假植苗进行处理，包括如下步骤：Described adopting 25% azoxystrobin suspension to process sugarcane tissue culture false planting, comprises the steps:
（1）甘蔗组培生根苗假植在苗床时，不淋定根水，用25%嘧菌酯悬浮液1000-1200倍喷雾叶片，并使药液沿蔗苗到达根系；(1) When sugarcane tissue culture rooted seedlings are artificially planted in the seedbed, do not drench the root water, spray the leaves with 25% azoxystrobin suspension 1000-1200 times, and let the liquid reach the root system along the sugarcane seedlings;
（2）假植喷药后1周左右，用25%嘧菌酯悬浮液1200-1500倍液加0.2％-0.4％磷酸二氢钾喷雾，连喷1-2次。(2) About 1 week after spraying the fake plants, spray 1200-1500 times of 25% azoxystrobin suspension plus 0.2%-0.4% potassium dihydrogen phosphate, and spray 1-2 times.
本发明突出的技术效果在于：The outstanding technical effects of the present invention are:
利用嘧菌酯对病菌强大的预防兼治疗作用，增强蔗苗的抗病能力，从而提高其成活率。方法简单，投资少，实用性强，组培生根苗丛栽成活率高，在低温潮湿梅雨季节成活率可达90％左右，其余节季成活率可达97％以上。Utilize the strong preventive and therapeutic effects of azoxystrobin on pathogens to enhance the disease resistance of sugarcane seedlings, thereby increasing their survival rate. The method is simple, low in investment, strong in practicability, and has a high survival rate of group planting of rooted seedlings by tissue culture. The survival rate can reach about 90% in the low temperature and humid plum rain season, and can reach more than 97% in other seasons.
具体实施方式Detailed ways
以下通过具体实施例对本发明的技术方案作进一步说明。The technical solution of the present invention will be further described below through specific examples.
实施例1Example 1
本发明所述的提高甘蔗组培生根苗假植成活率的方法，包括如下步骤：The method for improving the survival rate of rooted seedlings of sugarcane tissue culture of the present invention comprises the following steps:
1.在育苗大棚内，用干净细河沙或沙质生土铺成宽1.2-1.5米、厚8-12厘米、长根据大棚长度而定的育苗床；1. In the seedling greenhouse, use clean fine river sand or sandy soil to pave the seedling bed with a width of 1.2-1.5 meters, a thickness of 8-12 cm, and a length determined according to the length of the greenhouse;
2.用0.1%～0.5%高锰酸钾溶液喷洒浇透，用薄膜盖闷床土2～3d。2. Spray and water thoroughly with 0.1% to 0.5% potassium permanganate solution, and cover the stuffy bed soil with a film for 2 to 3 days.
3.组培生根苗按常规炼苗处理2-3天后，把生根苗从瓶子或袋子取出后，分成若干小丛，每丛苗3-5株，用清水洗干净附在苗上的培养基，在阴凉处滤干水；3. After 2-3 days of conventional seedling hardening, take the rooted seedlings out of the bottle or bag and divide them into small clumps, with 3-5 seedlings in each clump. Wash the medium attached to the seedlings with clean water , drain the water in the shade;
4.生根苗丛栽行距7-8cm、丛距4-5cm，栽后用有效成分25%嘧菌酯悬浮液1100倍喷雾，并使药液沿蔗苗到达根系；4. The rooting seedlings are planted with a row spacing of 7-8cm and a cluster spacing of 4-5cm. After planting, spray with 25% azoxystrobin suspension 1100 times the active ingredient, and let the liquid reach the root system along the sugarcane seedlings;
5.在苗床上搭拱盖膜，使苗生长环境空气湿度达90％-100％；5. Build an arched film on the seedbed to make the air humidity of the seedling growth environment reach 90%-100%;
6.丛栽喷药后1周左右，用有效成分25%嘧菌酯悬浮液1200倍液加0.2％-0.4％磷酸二氢钾喷雾1次；2周后用有效成分25%嘧菌酯悬浮液1500倍液加0.2％-0.4％磷酸二氢钾喷雾1次。6. About 1 week after cluster planting spraying, use 1200 times of active ingredient 25% azoxystrobin suspension plus 0.2%-0.4% potassium dihydrogen phosphate to spray once; 2 weeks later, use active ingredient 25% azoxystrobin to suspend Add 0.2%-0.4% potassium dihydrogen phosphate to 1500 times of solution and spray once.
7.组培苗成活后，去除拱膜，按正常管理，待蔗苗假茎高10-15cm时分单株进行营养杯假植。7. After the tissue-cultured seedlings survived, remove the arch membrane and manage as normal. When the pseudo-stem height of the sugarcane seedlings is 10-15 cm high, the individual plants will be planted in nutritional cups.
8．实施效果8. Implementation Effect
实施例2Example 2
本发明所述的提高甘蔗组培生根苗假植成活率的方法，包括如下步骤：The method for improving the survival rate of rooted seedlings of sugarcane tissue culture of the present invention comprises the following steps:
1.在育苗大棚内，用干净细河沙或沙质生土铺成宽1.2-1.5米、厚8-12厘米、长根据大棚长度而定的育苗床；1. In the seedling greenhouse, use clean fine river sand or sandy soil to pave the seedling bed with a width of 1.2-1.5 meters, a thickness of 8-12 cm, and a length determined according to the length of the greenhouse;
2.用干净的自来水喷淋，使河沙或沙土的含水量保持在田间最大持水量的70％左右；2. Spray with clean tap water to keep the water content of river sand or sandy soil at about 70% of the maximum water holding capacity in the field;
3.组培生根苗按常规炼苗处理2-3天后，把生根苗从瓶子或袋子取出后，分成若干小丛，每丛苗3-5株，用清水洗干净附在苗上的培养基，在阴凉处滤干水；3. After 2-3 days of conventional seedling hardening, take the rooted seedlings out of the bottle or bag and divide them into small clumps, with 3-5 seedlings in each clump. Wash the medium attached to the seedlings with clean water , drain the water in the shade;
4.生根苗丛栽行距7-8cm、丛距4-5cm，栽后用有效成分25%阿米西达悬浮液1000倍喷雾，并使药液沿蔗苗到达根系；4. The rooted seedlings are planted with a row spacing of 7-8cm and a cluster spacing of 4-5cm. After planting, spray 1000 times the active ingredient 25% amida suspension, and let the liquid reach the root system along the sugarcane seedlings;
5.在苗床上搭拱盖膜，使苗生长环境空气湿度达90％-100％；5. Build an arched film on the seedbed to make the air humidity of the seedling growth environment reach 90%-100%;
6.在6-10月份用透光率75％左右的遮阳网覆盖；6. Cover with a sunshade net with a light transmittance of about 75% from June to October;
7.丛栽喷药后1周左右，用有效成分25%阿米西达悬浮液1500倍液加0.2％-0.4％磷酸二氢钾喷雾1次。7. About 1 week after the cluster planting spraying, spray 1 time with 1500 times of active ingredient 25% amida suspension plus 0.2%-0.4% potassium dihydrogen phosphate.
8.组培苗成活后，去除拱膜和遮阳网，按正常管理，待蔗苗假茎高10-15cm时分单株进行营养杯假植。8. After the tissue culture seedlings survive, remove the arch film and sunshade net, and manage as normal. When the pseudostem height of the sugarcane seedlings is 10-15cm high, the individual plants will be planted in nutritional cups.
9．实施效果9. Implementation Effect
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104303788A (en) * | 2014-10-25 | 2015-01-28 | 云南省农业科学院甘蔗研究所 | Sugarcane seedling floating heel-in method |
| CN105191610A (en) * | 2015-08-24 | 2015-12-30 | 太仓市丰缘农场专业合作社 | Method for high-yield planting of sugarcanes |
| CN106332648A (en) * | 2016-08-30 | 2017-01-18 | 广西壮族自治区农业科学院甘蔗研究所 | Single-plant temporary planting method of sugarcane tissue culture rooting bottle seedling |
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| CN103025162A (en) * | 2010-06-09 | 2013-04-03 | 巴斯夫欧洲公司 | Method for cultivating sugar cane |
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- 2013-06-20 CN CN2013102484777A patent/CN103355141A/en active Pending
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| CN103025162A (en) * | 2010-06-09 | 2013-04-03 | 巴斯夫欧洲公司 | Method for cultivating sugar cane |
Non-Patent Citations (2)
| Title |
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| 姜美辉等: "香蕉组培苗假植管理技术", 《中国热带农业》 * |
| 董立华等: "甘蔗芽培苗一、二级苗圃管理技术", 《甘蔗》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104303788A (en) * | 2014-10-25 | 2015-01-28 | 云南省农业科学院甘蔗研究所 | Sugarcane seedling floating heel-in method |
| CN105191610A (en) * | 2015-08-24 | 2015-12-30 | 太仓市丰缘农场专业合作社 | Method for high-yield planting of sugarcanes |
| CN106332648A (en) * | 2016-08-30 | 2017-01-18 | 广西壮族自治区农业科学院甘蔗研究所 | Single-plant temporary planting method of sugarcane tissue culture rooting bottle seedling |
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Application publication date: 20131023 |