CN103340322B - Feed additive for effectively enhancing pig immunity and preparation method thereof - Google Patents
Feed additive for effectively enhancing pig immunity and preparation method thereof Download PDFInfo
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Abstract
The invention provides a feed additive for effectively enhancing pig immunity. The feed additive comprises a houttuynia cordata extract, liquorice, acanthopanax, vitamin C, calcium pantothenate, phenylalanine, choline chloride and the balance of a carrier, wherein the mass ratio of the carrier to wheat bran and degreased rice bran is 1:1. The feed additive has the advantages that the morbidity of pigs is remarkably reduced, the immunity of the pigs is improved, and the application of chemical synthetic additives in a feed can be replaced or partially replaced. The invention further provides a method for efficiently extracting effective ingredients of houttuynia cordata, and the method has the characteristics of high efficiency, simplicity, convenience, rapidness and the like.
Description
Technical field
The present invention relates to a kind of feed addictive and preparation method thereof, the feed addictive that to relate in particular to by houttuynia extract be main component and the preparation method of cordate houttuynia active ingredient, belong to feed additive field.
Background technology
Along with the flourish and people of China's aquaculture are more and more higher to the attention rate of Pork Security, the feed addictive that research and develop pollution-free, noresidue, green safety, does not develop immunity to drugs has become the hot issue of research, is also the trend of future in feed industry development.At present, the additive using in feedstuff industry still mainly take chemical synthesis as main, these additives are more or less with and are served side effect, can remain in pig body or some bacterium in pig body is developed immunity to drugs, finally by food chain, to human body, bring harm in various degree, the material of developing alternative or part instead of chemical additive seems particularly important.Chinese herbal medicine is as the rarity of China, and the utilization in feed is with a long history, compares and also has unique advantage with chemical synthesis additive.Resources of Chinese medicinal herb is in natural plants, can not produce medicament residue, can not cause bacterium, virus variation, free from environmental pollution etc. feature, can play and substitute or the effect of part alternative medicine additive, antibiotic etc.
The traditional Chinese herbal medicine ，Bei Ministry of Public Health of cordate houttuynia ，Shi China is defined as one of the kind of " medicine-food two-purpose ", is Saururaceae herbaceos perennial, and on the south the Yangtze river basin, all there is growth each province, rubs with the hands after broken and has a fish like smell, therefore named cordate houttuynia because of its cauline leaf.It is rich in volatile oil compound, flavone compound, water-soluble polysaccharide, strikes the materials such as dish alkali, organic acid compounds and many alcohol compounds, has the physiological and pharmacological such as antibacterial, antiviral, antiallergy and adjusting immunity active.About cordate houttuynia, as additive, have been reported at present, but major part is all directly to add after simple process, process like this and cause the large palatability that affects feed of cordate houttuynia consumption, absorb slow, active ingredient and be difficult for absorption, onset is slow etc.; Also after having report to adopt hot water extracting, add, process like this utilization that is more conducive to cordate houttuynia active ingredient than direct interpolation, but adopt, extraction effect is poor in this way, the recovery rate of active ingredient is low, be unfavorable for effective utilization of cordate houttuynia plant, waste resource, studies a kind of effective method of extracting cordate houttuynia functional component, can not only mention the comprehensive utilization value of cordate houttuynia, also can provide more rich in natural resources for feed addictive.
Summary of the invention
The object of this invention is to provide a kind of feed addictive that is main component by houttuynia extract, this feed addictive can effectively strengthen pig immunity, promotes pig weightening finish, increases culture benefit.
Another object of the present invention is to provide a kind of concrete extracting method of high efficiency extraction cordate houttuynia active ingredient.
The present invention is achieved through the following technical solutions:
A kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is according to the following ratio: houttuynia extract 10-15 gram, Radix Glycyrrhizae 3-5 gram, wilsonii 3-5 gram, vitamin C 0.2-0.4 gram, calcium pantothenate 0.3-0.6 gram, phenylalanine 0.4-0.7 gram, Choline Chloride 0.6-0.9 gram, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio.
Described Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves.
Described houttuynia extract, obtains by following preparation method:
Step 1: gather cordate houttuynia, adopt 50-60 ℃ of forced air drying mode to be dried processing, the dry rear plant pulverizer that uses is pulverized, and crosses 40 mesh sieves, obtains powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that pretreatment is good packs in ultrasonic extractor, adds the ethanol of 10-15 times of 60%-80%, ultrasonic 30-40 minute, ultrasonic power is 200-220W;
Step 3: ultrasonic extraction finishes rear employing vacuum filtration, obtains filtrate and filter residue, and filtrate is the 1/5-1/6 to original volume by Vacuum Concentration, thickening temperature is 45-65 ℃, ethanol is reclaimed simultaneously; Filter residue extracts for the second time by the mode of step 2;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, by filtrate by Vacuum Concentration the 1/5-1/6 to original volume, thickening temperature is 45-65 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration by deionized water, 6000 revs/min of lower refrigerated centrifuges 5 minutes, gets supernatant and add by solid-to-liquid ratio 1:2 the active carbon that pretreatment is good, and frequently stir, continue 1-1.5 hour, then vacuum filtration, collects filtrate;
The method of described Activated Carbon Pretreatment: by solid-to-liquid ratio 1:(3-4) in active carbon, add deionized water, keep 60-80 ℃, maintain 0.5h, then filter, get filter residue, then be 1:(2-3 by solid-to-liquid ratio) add 4% watery hydrochloric acid, constantly stir and maintain 20-30 minute, then by deionized water, be flushed into neutrality, put into afterwards baking oven 60-70 ℃ of oven dry;
Step 6: filtrate is added in knockout, then add ethyl acetate to extract, collect ethyl acetate extract layer, then Vacuum Concentration carries out freeze drying to finite concentration, freeze drying condition is vacuum 10 Pa, and temperature-50, to-60 ℃, obtain cordate houttuynia effective component extracts dry powder.
The active carbon of processing through the present invention, there is good physics and chemistry adsorption effect, the effectively pigment in adsorbing and extracting liquid and impurity component etc., can also optionally adsorb the little composition of some physiological actions, thereby make in extract the content of active component higher, and active carbon can adsorb the taste in cordate houttuynia, be conducive to improve the palatability of feed.
Houttuynia extract purity after above-mentioned PROCESS FOR TREATMENT is high, physiologically active is strong, there is good ph stability and heat endurance simultaneously, through related experiment, learn that houttuynia extract that the method makes adds in pig feed and can effectively improve the immunity of pig, reduce the pig incidence of disease.Method before comparing in document is extracted, and its physiologically active has obtained larger raising, and the consumption that can reduce approximately half just can reach same effect.
In pig feed per ton, adding 300-500 gram of this additive mixes, granulates, packs.
The effect of cordate houttuynia active ingredient in the present invention: the houttuynia extract main component obtaining by the extraction in the present invention and separation method is the composition that flavonoids, organic acid etc. have physiologically active, having the free radical of removing, inhibiting bacteria and diminishing inflammation agent enhancing immunity energy function, is a kind of outstanding natural health-care products.
The present invention compared with prior art, has the following advantages and effect:
In this feed addictive, contain various active composition, comprise houttuynia extract, Radix Glycyrrhizae, wilsonii, vitamin C, calcium pantothenate, phenylalanine, Choline Chloride etc., be of high nutritive value, compatibility science, can effectively improve the immunity of pig, reduce the pig incidence of disease, avoided use chemical synthesis additive (as antibiotic etc.) simultaneously, there is side effect little, safety, the advantages such as environmental protection, are conducive to promote feed and the healthy and orderly development of livestock, human health is had to positive effect, belong to the feed addictive of safe green.The method that the invention provides in addition cordate houttuynia active ingredient has the advantages such as convenient, fast, simple, efficient, can greatly improve the purity of cordate houttuynia active ingredient, be conducive to improve the quality of feed, avoided some adiaphorous impurity components to add in feed and affected the final quality of feed.
Specific embodiment
By specific embodiment, further describe the present invention below.
Embodiment 1: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is according to the following ratio: 12.5 grams of houttuynia extracts, 4 grams, Radix Glycyrrhizae, 4 grams of wilsoniis, 0.3 gram of vitamin C, 0. 45 grams of calcium pantothenates, 0.55 gram of phenylalanine, 0.75 gram of Choline Chloride, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves.Wherein, in pig feed per ton, adding 400 grams of these additives mixes, granulates, packs.
Described houttuynia extract obtains in the following manner:
Step 1: gather cordate houttuynia, adopt 55 ℃ of forced air drying modes to be dried processing, the dry rear plant pulverizer that uses is pulverized, and crosses 40 mesh sieves, obtains powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that pretreatment is good packs in ultrasonic extractor, adds the ethanol of 13 times 70%, ultrasonic 35 minutes, ultrasonic power was 210W;
Step 3: ultrasonic extraction finishes rear employing vacuum filtration, obtains filtrate and filter residue, filtrate is by Vacuum Concentration to 1/5 of original volume, and thickening temperature is 55 ℃, reclaims ethanol simultaneously; Filter residue extracts for the second time by the mode of step 2;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, by filtrate by Vacuum Concentration to by filtrate by Vacuum Concentration to 1/5 of original volume, thickening temperature is 55 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration by deionized water, 6000 revs/min of lower refrigerated centrifuges 5 minutes, gets supernatant and add by solid-to-liquid ratio 1:2 the active carbon that pretreatment is good, and frequently stir, continue 1.3 hours, then vacuum filtration, collects filtrate;
The pretreatment of active carbon: add deionized water by solid-to-liquid ratio 1:4 in active carbon, keep 70 ℃, maintain 0.5h, then filter, get filter residue, then add 4% watery hydrochloric acid by solid-to-liquid ratio 1:3, constantly stir and maintain 30 minutes, then by deionized water, be flushed into neutrality, put into afterwards 70 ℃ of oven dry of baking oven;
Step 6: filtrate is added in knockout, then add ethyl acetate to extract, collect ethyl acetate extract layer, then carry out Vacuum Concentration and carry out freeze drying to finite concentration, freeze drying condition is vacuum 10 Pa, and temperature-55 ℃ obtain cordate houttuynia effective component extracts dry powder.
Embodiment 2: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is according to the following ratio: 10 grams of houttuynia extracts, 3 grams, Radix Glycyrrhizae, 3 grams of wilsoniis, 0.2 gram of vitamin C, 0.3 gram of calcium pantothenate, 0.4 gram of phenylalanine, 0.6 gram of Choline Chloride, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves.Wherein, in pig feed per ton, adding 300 grams of these additives mixes, granulates, packs.
Described houttuynia extract obtains in the following manner:
Step 1: gather cordate houttuynia, adopt 50 ℃ of forced air drying modes to be dried processing, the dry rear plant pulverizer that uses is pulverized, and crosses 40 mesh sieves, obtains powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that obtains that pretreatment is good packs in ultrasonic extractor, adds the ethanol of 10 times 60%, and ultrasonic 30 minutes, ultrasonic power was 200W;
Step 3: ultrasonic extraction finishes rear employing vacuum filtration, obtains filtrate and filter residue, filtrate is by Vacuum Concentration to 1/5 of original volume, and thickening temperature is 45 ℃, ethanol is reclaimed simultaneously; Filter residue extracts for the second time by the mode of step 2;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, by filtrate by Vacuum Concentration to 1/5 of original volume, thickening temperature is 45 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration by deionized water, 6000 revs/min of lower refrigerated centrifuges 5 minutes, gets supernatant and add by solid-to-liquid ratio 1:2 the active carbon that pretreatment is good, and frequently stir, continue 1 hour, then vacuum filtration, collects filtrate;
The pretreatment of active carbon: add deionized water by solid-to-liquid ratio 1:3 in active carbon, keep 60 ℃, maintain 0.5h, then filter, get filter residue, then add 4% watery hydrochloric acid by solid-to-liquid ratio 1:2, constantly stir and maintain 20 minutes, then by deionized water, be flushed into neutrality, put into afterwards 60 ℃ of oven dry of baking oven;
Step 6: filtrate is added in knockout, then add ethyl acetate to extract, collect ethyl acetate extract layer, then Vacuum Concentration carries out freeze drying to finite concentration, freeze drying condition is vacuum 10 Pa, and temperature-50 ℃ obtain cordate houttuynia effective component extracts dry powder.
Embodiment 3: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is according to the following ratio: 15 grams of houttuynia extracts, 5 grams, Radix Glycyrrhizae, 5 grams of wilsoniis, 0.4 gram of vitamin C, 0.6 gram of calcium pantothenate, 0.7 gram of phenylalanine, 0.9 gram of Choline Chloride, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves.Wherein, in pig feed per ton, adding 500 grams of these additives mixes, granulates, packs.
Described houttuynia extract obtains in the following manner:
Step 1: gather cordate houttuynia, adopt 60 ℃ of forced air drying modes to be dried processing, the dry rear plant pulverizer that uses is pulverized, and crosses 40 mesh sieves, obtains powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that obtains that pretreatment is good packs in ultrasonic extractor, adds the ethanol of 15 times 80%, and ultrasonic 40 minutes, ultrasonic power was 220W;
Step 3: ultrasonic extraction finishes rear employing vacuum filtration, obtains filtrate and filter residue, filtrate is by Vacuum Concentration to 1/6 of original volume, and thickening temperature is 65 ℃, ethanol is reclaimed simultaneously; Filter residue extracts for the second time by the mode of step 2;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, by filtrate by Vacuum Concentration to 1/6 of original volume, thickening temperature is 65 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration by deionized water, 6000 revs/min of lower refrigerated centrifuges 5 minutes, gets supernatant and add by solid-to-liquid ratio 1:2 the active carbon that pretreatment is good, and frequently stir, continue 1.5 hours, then vacuum filtration, collects filtrate;
The pretreatment of active carbon: add deionized water by solid-to-liquid ratio 1:4 in active carbon, keep 80 ℃, maintain 0.5h, then filter, get filter residue, then add 4% watery hydrochloric acid by solid-to-liquid ratio 1:2, constantly stir and maintain 30 minutes, then by deionized water, be flushed into neutrality, put into afterwards 70 ℃ of oven dry of baking oven;
Step 6: filtrate is added in knockout, then add ethyl acetate to extract, collect ethyl acetate extract layer, then Vacuum Concentration carries out freeze drying to finite concentration, freeze drying condition is vacuum 10 Pa, and temperature-60 ℃ obtain cordate houttuynia effective component extracts dry powder.
Embodiment 4: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is according to the following ratio: 12.5 grams of houttuynia extracts, 4 grams, Radix Glycyrrhizae, 4 grams of wilsoniis, 0.3 gram of vitamin C, 0. 45 grams of calcium pantothenates, 0.55 gram of phenylalanine, 0.75 gram of Choline Chloride, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves.Wherein, in pig feed per ton, adding 400 grams of these additives mixes, granulates, packs.
Described houttuynia extract obtains in the following manner:
Step 1: gather cordate houttuynia, adopt 90 ℃ of forced air drying modes to be dried processing, the dry rear plant pulverizer that uses is pulverized, and crosses 40 mesh sieves;
Step 2: the good raw material of pretreatment is packed in ultrasonic extractor, add the ethanol of 5 times 40%, ultrasonic 15 minutes, ultrasonic power was 100W;
Step 3: ultrasonic extraction finishes rear employing vacuum filtration, obtains filtrate and filter residue, filtrate is by Vacuum Concentration to 1/8 of original volume, and thickening temperature is 80 ℃, reclaims ethanol simultaneously; Filter residue extracts for the second time by the mode of step 2;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, by filtrate by Vacuum Concentration to finite concentration, merge for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration by deionized water, 6000 revs/min of lower refrigerated centrifuges 5 minutes, gets supernatant and add by solid-to-liquid ratio 1:2 the active carbon that pretreatment is good, and frequently stir, continue 0.5 hour, then vacuum filtration, collects filtrate;
The pretreatment of active carbon: add deionized water by solid-to-liquid ratio 1:2 in active carbon, keep 40 ℃, maintain 0.5h, then filter, get filter residue, then add 4% watery hydrochloric acid by solid-to-liquid ratio 1:2, constantly stir and maintain 10 minutes, then by deionized water, be flushed into neutrality, put into afterwards 60 ℃ of oven dry of baking oven;
Step 6: filtrate is added in knockout, add again ethyl acetate to extract, collect ethyl acetate extract layer, then carry out Vacuum Concentration and carry out freeze drying to finite concentration, freeze drying condition is vacuum 7 Pa, and temperature-35 ℃ obtain cordate houttuynia effective component extracts dry powder.
Embodiment 5: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is according to the following ratio: 7 grams of houttuynia extracts, 2 grams, Radix Glycyrrhizae, 2.5 grams of wilsoniis, 0.1 gram of vitamin C, 0.2 gram of calcium pantothenate, 0.35 gram of phenylalanine, 0.55 gram of Choline Chloride, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves.Wherein, in pig feed per ton, adding 200 grams of these additives mixes, granulates, packs.
All the other are with embodiment 1
Embodiment 6: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is according to the following ratio: 7 grams of houttuynia extracts, 2 grams, Radix Glycyrrhizae, 2.5 grams of wilsoniis, 0.1 gram of vitamin C, 0.2 gram of calcium pantothenate, 0.35 gram of phenylalanine, 0.55 gram of Choline Chloride, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves.Wherein, in pig feed per ton, adding 200 grams of these additives mixes, granulates, packs.
All the other are with embodiment 4.
Embodiment 7: every hectogram feed addictive according to the following ratio: 12.5 grams of houttuynia extracts, 4 grams, Radix Glycyrrhizae, 4 grams of wilsoniis, 0.3 gram of vitamin C, 0. 45 grams of calcium pantothenates, 0.55 gram of phenylalanine, 0.75 gram of Choline Chloride, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 100 mesh sieves.Wherein, in pig feed per ton, adding 400 grams of these additives mixes, granulates, packs.
Described houttuynia extract extracting method is referring to the extracting method of a Chinese invention patent CN 101085096B cordate houttuynia of Granted publication: adopt conventional extraction method, gather cordate houttuynia, adopt forced air drying mode to be dried processing, the dry rear plant pulverizer that uses is pulverized, and crosses 40 mesh sieves.The alcohol extract that is 70% by volumetric concentration under the condition of room temperature, stirring by powder cordate houttuynia 3 hours, the weight/volume of cordate houttuynia and ethanol is 1:15, filters, Vacuum Concentration freeze drying, obtains houttuynia extract.
Test example:
1, experimental animal and experimental design
640 of the health pig that test selection Guangnan (Zhanjiang) subordinate plant of Jia Feng feed corporation,Ltd average weight is 85.1 ± 2.2kg, statistical analysis body weight difference is not remarkable.Be divided at random eight groups, every group of 8 repetitions, each repeats 10 male and females half and half, comprising a blank group and 7 test group.In blank group swine rations, do not add the additive of any enhancing pig immunity making in embodiment 1-7, in all the other 7 test group swine rationses, add respectively the additive that embodiment 1-7 makes, live pig free choice feeding and drinking-water, carry out routinely immunity and expelling parasite, in experimental period, observe and record the mental status of swine flu situation and pig every day.Totally 30 days experimental period.During off-test, weigh, and ramming material.Record swine flu incidence.Calculate each processed group daily gain, feed intake, feedstuff-meat ratio (feed intake/weightening finish) and the incidence of disease.
Data statistic analysis
Test data adopts SSPS19.0 statistical software to carry out statistical analysis, the results are shown in following table:
| Project | Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 |
| All the time (kg) | 84.2 | 84.8 | 85.2 | 86.2 | 85.2 | 85 | 84.2 | 85.6 |
| Not heavy (kg) | 108.4 | 111.3 | 112.1 | 112.5 | 110.3 | 110.4 | 109.1 | 110.4 |
| Daily gain (g/d head) | 761 | 892 | 868 | 837 | 828 | 846 | 838 | 806 |
| Daily ingestion amount (g/d head) | 2452 | 2599 | 2622 | 2560 | 2591 | 2578 | 2612 | 2561 |
| Feedstuff-meat ratio | 3.22 | 2.91 | 3.02 | 3.06 | 3.13 | 3.05 | 3.12 | 3.17 |
| The incidence of disease | 12.3% | 1.5% | 2.1% | 1.9% | 5.2% | 4.7% | 6.3% | 7.2% |
Feed intake (ADFI): the feeding capacity of each hurdle test pig of accurate recording and clout amount, calculate every pig average daily ingestion amount and complete average daily ingestion amount of phase weekly;
Daily gain (ADG): test pig starts weekly and finish morning 7:30 weighs on an empty stomach, calculates average daily gain and the full phase average daily gain weekly of every pig;
Feedstuff-meat ratio (F/G): calculate according to feed intake and weightening finish.
Disease symptom: sick pig suddenly heating reaches 42 ℃ even higher, and ight soil is dry and hard, lassitude, anorexia or do not eat, normal accumbency together, is reluctant activity, expiratory dyspnea, cough, eye, nose stream mucus.
Conclusion: result of the test shows, the feed addictive basal feed that the present invention makes is compared, and has significantly reduced the incidence of disease of pig, has strengthened the immunity of pig, simultaneously also growth rate, daily gain to a certain extent.By comparing with embodiment 7, the active ingredient that the method for extraction cordate houttuynia active ingredient used in the present invention is extracted than commonsense method is added the better effects if obtaining in feed to, the incidence of disease of pig is lower, the cordate houttuynia active ingredient purity that extracting method extraction of the present invention is described is higher, more can guarantee the abundant extraction of cordate houttuynia active ingredient.
This products material is green safety material, and can significantly reduce the incidence of disease of pig, and strengthen the immunity of pig, can be used as antibiotic substitute and add in pig feed, produce the good mixed feed of disease resisting effect, there are good using value and wide market prospects.
Claims (2)
1. a feed addictive that effectively strengthens pig immunity, it is characterized in that: every hectogram feed addictive according to the following ratio: houttuynia extract 10-15 gram, Radix Glycyrrhizae 3-5 gram, wilsonii 3-5 gram, vitamin C 0.2-0.4 gram, calcium pantothenate 0.3-0.6 gram, phenylalanine 0.4-0.7 gram, Choline Chloride 0.6-0.9 gram, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio;
Described houttuynia extract preparation method is:
Step 1: gather cordate houttuynia, adopt 50-60 ℃ of forced air drying mode to be dried processing, the dry rear plant pulverizer that uses is pulverized, and crosses 40 mesh sieves, obtains powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that pretreatment is good packs in ultrasonic extractor, adds the ethanol of 10-15 times of 60%-80%, ultrasonic 30-40 minute, ultrasonic power is 200-220W;
Step 3: ultrasonic extraction finishes rear employing vacuum filtration, obtains filtrate and filter residue, and filtrate is the 1/5-1/6 to original volume by Vacuum Concentration, thickening temperature is 45-65 ℃, ethanol is reclaimed simultaneously; Filter residue extracts for the second time by the mode of step 2;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, by filtrate by Vacuum Concentration the 1/5-1/6 to original volume, thickening temperature is 45-65 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration by deionized water, 6000 revs/min of lower refrigerated centrifuges 5 minutes, gets supernatant and add by solid-to-liquid ratio 1:2 the active carbon that pretreatment is good, and frequently stir, continue 1-1.5 hour, then vacuum filtration, collects filtrate; The pretreatment mode of described active carbon is: by solid-to-liquid ratio 1:(3-4) in active carbon, add deionized water, keep 60-80 ℃, maintain 0.5h, then filter, get filter residue, then by solid-to-liquid ratio 1:(2-3) add 4% watery hydrochloric acid, constantly stir and maintain 20-30 minute, then by deionized water, be flushed into neutrality, put into afterwards baking oven 60-70 ℃ of oven dry;
Step 6: filtrate is added in knockout, then add ethyl acetate to extract, collect ethyl acetate extract layer, then Vacuum Concentration carries out freeze drying to finite concentration, freeze drying condition is vacuum 10Pa, and temperature-50 ℃, to-60 ℃, obtain cordate houttuynia effective component extracts dry powder.
2. according to the feed addictive of a kind of effective enhancing pig immunity described in claim 1; every hectogram feed addictive is according to the following ratio: 12.5 grams of houttuynia extracts; 4 grams, Radix Glycyrrhizae; 4 grams of wilsoniis, 0.3 gram of vitamin C, 0.5 gram of calcium pantothenate; 0.4 gram of phenylalanine; 0.7 gram of Choline Chloride, all the other are carrier, carrier is wheat bran and defatted rice bran 1:1 in mass ratio;
Described houttuynia extract preparation method is:
Step 1: gather cordate houttuynia, adopt 55 ℃ of forced air drying modes to be dried processing, the dry rear plant pulverizer that uses is pulverized, and crosses 40 mesh sieves, obtains powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that pretreatment is good packs in ultrasonic extractor, adds the ethanol of 13 times 70%, ultrasonic 35 minutes, ultrasonic power was 210W;
Step 3: ultrasonic extraction finishes rear employing vacuum filtration, obtains filtrate and filter residue, filtrate is by Vacuum Concentration to 1/5 of original volume, and thickening temperature is 55 ℃, reclaims ethanol simultaneously; Filter residue extracts for the second time by the mode of step 2;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, by filtrate by Vacuum Concentration to by filtrate by Vacuum Concentration to 1/5 of original volume, thickening temperature is 55 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration by deionized water, 6000 revs/min of lower refrigerated centrifuges 5 minutes, gets supernatant and add by solid-to-liquid ratio 1:2 the active carbon that pretreatment is good, and frequently stir, continue 1.3 hours, then vacuum filtration, collects filtrate; The pretreatment mode of described active carbon is: by solid-to-liquid ratio 1:4, add deionized water in active carbon, keep 70 ℃, maintain 0.5h, then filter, get filter residue, then add 4% watery hydrochloric acid by solid-to-liquid ratio 1:3, constantly stir and maintain 30 minutes, then by deionized water, be flushed into neutrality, put into afterwards 70 ℃ of oven dry of baking oven;
Step 6: filtrate is added in knockout, then add ethyl acetate to extract, collect ethyl acetate extract layer, then carry out Vacuum Concentration and carry out freeze drying to finite concentration, freeze drying condition is vacuum 10Pa, and temperature-55 ℃ obtain cordate houttuynia effective component extracts dry powder.
3. a kind of feed addictive of effective enhancing pig immunity according to claim 1, the addition in pig feed is 300-500g/ ton.
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| CN103340322B true CN103340322B (en) | 2014-03-12 |
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| CN103652492B (en) * | 2013-12-16 | 2014-07-30 | 双胞胎（集团）股份有限公司 | Lactating sow feed additive capable of improving lactation yield and boosting immunity |
| CN106578362A (en) * | 2015-10-17 | 2017-04-26 | 饶平东泽生物科技有限公司 | Feed additive formula |
| CN107712347A (en) * | 2017-10-30 | 2018-02-23 | 金华傲农生物科技有限公司 | A kind of additive of compound feed for pig and preparation method and application |
| CN107667939A (en) * | 2017-11-17 | 2018-02-09 | 金陵科技学院 | Use the method for the bacteriostatic activity of Procambius clarkii checking plant extraction liquid bacterium |
| CN112056470A (en) * | 2020-09-17 | 2020-12-11 | 上海骑骠动物保健品有限公司 | Houttuynia cordata feed additive |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101007090A (en) * | 2006-01-24 | 2007-08-01 | 福建农林大学 | Health-preserving and growth-promoting Chinese medicinal additive for pig |
| CN102845635A (en) * | 2012-08-27 | 2013-01-02 | 广东海大集团股份有限公司 | Liver and body strengthening agent for Litopenaeus vannamei |
| CN102920911A (en) * | 2012-12-05 | 2013-02-13 | 胡海波 | Chinese medicament for treating keratoconjunctivitis sicca and preparation method thereof |
| CN103172064A (en) * | 2013-04-12 | 2013-06-26 | 宁夏大学 | Low-ash coal-based activated carbon quick activation production technology |
-
2013
- 2013-07-18 CN CN201310302051.5A patent/CN103340322B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101007090A (en) * | 2006-01-24 | 2007-08-01 | 福建农林大学 | Health-preserving and growth-promoting Chinese medicinal additive for pig |
| CN102845635A (en) * | 2012-08-27 | 2013-01-02 | 广东海大集团股份有限公司 | Liver and body strengthening agent for Litopenaeus vannamei |
| CN102920911A (en) * | 2012-12-05 | 2013-02-13 | 胡海波 | Chinese medicament for treating keratoconjunctivitis sicca and preparation method thereof |
| CN103172064A (en) * | 2013-04-12 | 2013-06-26 | 宁夏大学 | Low-ash coal-based activated carbon quick activation production technology |
Non-Patent Citations (2)
| Title |
|---|
| 复方中草药饲料添加剂对断奶仔猪生产性能的影响;季欣涛等;《科技信息(科学教研)》;20070810(第23期);293-295页 * |
| 季欣涛等.复方中草药饲料添加剂对断奶仔猪生产性能的影响.《科技信息(科学教研)》.2007,(第23期), |
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Effective date of registration: 20151228 Address after: 528100 C District, southwest science and Technology Industrial Park, Sanshui District, Guangdong, Foshan, China Patentee after: GUANGDONG TWINS FEED CO., LTD. Address before: 524045 Guangdong Province, Zhanjiang city Chikan North Road No. 2 Patentee before: Guangnan (Zhanjiang) Jiafeng Feed Co.,Ltd. |