CN103340322A - Feed additive for effectively enhancing pig immunity and preparation method thereof - Google Patents
Feed additive for effectively enhancing pig immunity and preparation method thereof Download PDFInfo
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Abstract
The invention provides a feed additive for effectively enhancing pig immunity. The feed additive comprises a houttuynia cordata extract, liquorice, acanthopanax, vitamin C, calcium pantothenate, phenylalanine, choline chloride and the balance of a carrier, wherein the mass ratio of the carrier to wheat bran and degreased rice bran is 1:1. The feed additive has the advantages that the morbidity of pigs is remarkably reduced, the immunity of the pigs is improved, and the application of chemical synthetic additives in a feed can be replaced or partially replaced. The invention further provides a method for efficiently extracting effective ingredients of houttuynia cordata, and the method has the characteristics of high efficiency, simplicity, convenience, rapidness and the like.
Description
Technical field
The present invention relates to a kind of feed addictive and preparation method thereof, relating in particular to by houttuynia extract is the feed addictive of main component and the preparation method of cordate houttuynia active ingredient, belongs to feed additive field.
Background technology
Along with the flourish and people of China's aquaculture are more and more higher to the attention rate of pork safety, the feed addictive that research and develop pollution-free, noresidue, green safety, does not develop immunity to drugs has become the hot issue of research, also is the trend of following feedstuff industry development.At present, the additive that uses in the feedstuff industry is still mainly based on chemical synthesis, these additives more or less bring some side effects, can remain in the pig body or some bacterium in the pig body is developed immunity to drugs, finally bring in various degree harm by food chain to human body, develop and substitute or the material of part instead of chemical additive seems particularly important.Chinese herbal medicine is as the rarity of China, and the utilization in feed is with a long history, and comparing with the chemical synthesis additive also has special advantages.Resources of Chinese medicinal herb is in natural plants, can not produce medicament residue, can not cause bacterium, virus variation, free from environmental pollution etc. characteristics, can play to substitute or the effect of part alternative medicine additive, antibiotic etc.
Cordate houttuynia is the traditional Chinese herbal medicine of China, is defined as one of the kind of " medicine-food two-purpose " by the Ministry of Public Health, is the Saururaceae herbaceos perennial, and all there is growth the each province on the south the Yangtze river basin, rubs with the hands because of its cauline leaf to have a fish like smell after broken, so the name cordate houttuynia.It is rich in volatile oil compound, flavone compound, water-soluble polysaccharide, strikes materials such as dish alkali, organic acid compounds and many alcohol compounds, has physiological and pharmacological activity such as antibiotic, antiviral, antiallergy and adjusting immunity.Report as additive is existing about cordate houttuynia at present, but major part all is directly interpolation after the simple process that processing causes big slow, the difficult absorption of active ingredient of palatability, absorption that influences feed of cordate houttuynia consumption like this, onset waits slowly; Add after also having report to adopt hot water extracting, handle the utilization that more is conducive to cordate houttuynia active ingredient than direct interpolation like this, but adopt this method extraction effect poor, extraction ratio of effective constituents is low, be unfavorable for effective utilization of cordate houttuynia plant, the waste resource is studied a kind of effective method of extracting the cordate houttuynia functional component, can not only mention the comprehensive utilization value of cordate houttuynia, also can provide more rich in natural resources for feed addictive.
Summary of the invention
The purpose of this invention is to provide a kind of is the feed addictive of main component by houttuynia extract, and this feed addictive can effectively strengthen pig immunity, promotes the pig weightening finish, increases culture benefit.
Another object of the present invention is to provide a kind of concrete extracting method of high efficiency extraction cordate houttuynia active ingredient.
The present invention is achieved through the following technical solutions:
A kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is pressed following proportioning: houttuynia extract 10-15 gram, Radix Glycyrrhizae 3-5 gram, wilsonii 3-5 gram, vitamin C 0.2-0.4 gram, calcium pantothenate 0.3-0.6 gram, phenylalanine 0.4-0.7 gram, Choline Chloride 0.6-0.9 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.
Described Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves and get final product.
Described houttuynia extract obtains by following preparation method:
Step 1: gather cordate houttuynia, adopt 50-60 ℃ of forced air drying mode to carry out drying and handle, dry back uses the plant pulverizer to pulverize, and crosses 40 mesh sieves, gets the powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that preliminary treatment is good is packed in the ultrasonic extractor, adds the ethanol of 10-15 times of 60%-80%, and ultrasonic 30-40 minute, ultrasonic power was 200-220W;
Step 3: ultrasonic extraction finishes the back and adopts vacuum filtration, obtains filtrate and filter residue, and filtrate is concentrated into the 1/5-1/6 of original volume by vacuum, and thickening temperature is 45-65 ℃, simultaneously ethanol is reclaimed; Filter residue carries out the second time with the mode of step 2 and extracts;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, filtrate is concentrated into the 1/5-1/6 of original volume by vacuum, thickening temperature is 45-65 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration with deionized water, 6000 rev/mins of following refrigerated centrifuges 5 minutes, gets supernatant and add the good active carbon of preliminary treatment by solid-to-liquid ratio 1:2, and stir frequently, continue 1-1.5 hour, vacuum filtration is collected filtrate then;
The method of described Activated Carbon Pretreatment: by solid-to-liquid ratio 1:(3-4) in active carbon, adds deionized water, keep 60-80 ℃, keep 0.5h, filter then, getting filter residue, is 1:(2-3 by solid-to-liquid ratio again) add 4% watery hydrochloric acid, constantly stir and kept 20-30 minute, with deionized water it is washed into neutrality then, put into baking oven 60-70 ℃ of oven dry afterwards and get final product;
Step 6: filtrate is added in the knockout, add ethyl acetate again and extract, collect the ethyl acetate extraction layer, vacuum is carried out freeze drying after being concentrated into finite concentration then, the freeze drying condition is vacuum 10 Pa, and temperature-50 obtains cordate houttuynia effective component extracts dry powder to-60 ℃.
Active carbon through the present invention's processing, have better physical and chemisorbed effect, the effectively pigment in the adsorbing and extracting liquid and impurity component etc., can also optionally adsorb the little composition of some physiological actions, thereby make that the content of active component is higher in the extract, and active carbon can adsorb the taste in the cordate houttuynia, is conducive to improve the palatability of feed.
Through the houttuynia extract purity height after the above-mentioned PROCESS FOR TREATMENT, physiologically active is strong, have good ph stability and heat endurance simultaneously, learn through related experiment that houttuynia extract that this method makes adds in the pig feed and can effectively improve the immunity of pig, reduce the pig incidence of disease.Method before comparing in the document is extracted, and its physiologically active has obtained bigger raising, can reduce and make an appointment with the consumption of half just can reach same effect.
Add 300-500 in the pig feed per ton and restrain that this additive mixes, granulates, packing gets final product.
The effect of cordate houttuynia active ingredient among the present invention: the houttuynia extract main component that obtains by the extraction among the present invention and separation method is the composition that flavonoids, organic acid etc. have physiologically active, having the free radical of removing, inhibiting bacteria and diminishing inflammation agent enhancing immunity energy function, is a kind of outstanding natural health-care products.
The present invention compared with prior art has the following advantages and effect:
Contain the various active composition in this feed addictive, comprise houttuynia extract, Radix Glycyrrhizae, wilsonii, vitamin C, calcium pantothenate, phenylalanine, Choline Chloride etc., be of high nutritive value, the compatibility science, can effectively improve the immunity of pig, reduce the pig incidence of disease, avoided use chemical synthesis additive (as antibiotic etc.) simultaneously, it is little to have side effect, safety, advantages such as environmental protection are conducive to promote feed and the healthy and orderly development of herding industry, human health there is positive effect, belongs to the feed addictive of safe green.The method that the invention provides cordate houttuynia active ingredient in addition has advantages such as convenient, fast, simple, efficient, can greatly improve the purity of cordate houttuynia active ingredient, be conducive to improve the quality of feed, avoided some adiaphorous impurity components to add in the feed and influence the final quality of feed.
Specific embodiment
Below further describe the present invention by specific embodiment.
Embodiment 1: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is pressed following proportioning: houttuynia extract 12.5 grams, Radix Glycyrrhizae 4 grams, wilsonii 4 grams, vitamin C 0.3 gram, calcium pantothenate 0. 45 grams, phenylalanine 0.55 gram, Choline Chloride 0.75 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves and get final product.Wherein, add in the pig feed per ton that 400 these additives of gram mix, granulate, packing gets final product.
Described houttuynia extract obtains in the following manner:
Step 1: gather cordate houttuynia, adopt 55 ℃ of forced air drying modes to carry out drying and handle, dry back uses the plant pulverizer to pulverize, and crosses 40 mesh sieves, gets the powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that preliminary treatment is good is packed in the ultrasonic extractor, adds 13 times 70% ethanol, and ultrasonic 35 minutes, ultrasonic power was 210W;
Step 3: ultrasonic extraction finishes the back and adopts vacuum filtration, obtains filtrate and filter residue, and filtrate is concentrated into 1/5 of original volume by vacuum, and thickening temperature is 55 ℃, reclaims ethanol simultaneously; Filter residue carries out the second time with the mode of step 2 and extracts;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, filtrate is concentrated into by vacuum filtrate is concentrated into 1/5 of original volume by vacuum, thickening temperature is 55 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration with deionized water, 6000 rev/mins of following refrigerated centrifuges 5 minutes, gets supernatant and add the good active carbon of preliminary treatment by solid-to-liquid ratio 1:2, and stir frequently, continue 1.3 hours, vacuum filtration is collected filtrate then;
The preliminary treatment of active carbon: in active carbon, add deionized water by solid-to-liquid ratio 1:4, keep 70 ℃, keep 0.5h, filter then, get filter residue, add 4% watery hydrochloric acid again by solid-to-liquid ratio 1:3, constantly stir and kept 30 minutes, with deionized water it is washed into neutrality then, put into 70 ℃ of oven dry of baking oven afterwards and get final product;
Step 6: filtrate is added in the knockout, add ethyl acetate again and extract, collect the ethyl acetate extraction layer, carry out then carrying out freeze drying after vacuum is concentrated into finite concentration, the freeze drying condition is vacuum 10 Pa, and temperature-55 ℃ obtains cordate houttuynia effective component extracts dry powder.
Embodiment 2: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is pressed following proportioning: houttuynia extract 10 grams, Radix Glycyrrhizae 3 grams, wilsonii 3 grams, vitamin C 0.2 gram, calcium pantothenate 0.3 gram, phenylalanine 0.4 gram, Choline Chloride 0.6 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves and get final product.Wherein, add in the pig feed per ton that 300 these additives of gram mix, granulate, packing gets final product.
Described houttuynia extract obtains in the following manner:
Step 1: gather cordate houttuynia, adopt 50 ℃ of forced air drying modes to carry out drying and handle, dry back uses the plant pulverizer to pulverize, and crosses 40 mesh sieves, gets the powdery cordate houttuynia;
Step 2: with preliminary treatment good the powdery cordate houttuynia is packed in the ultrasonic extractor, add 10 times 60% ethanol, ultrasonic 30 minutes, ultrasonic power was 200W;
Step 3: ultrasonic extraction finishes the back and adopts vacuum filtration, obtains filtrate and filter residue, and filtrate is concentrated into 1/5 of original volume by vacuum, and thickening temperature is 45 ℃, simultaneously ethanol is reclaimed; Filter residue carries out the second time with the mode of step 2 and extracts;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, filtrate is concentrated into 1/5 of original volume by vacuum, thickening temperature is 45 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration with deionized water, 6000 rev/mins of following refrigerated centrifuges 5 minutes, gets supernatant and add the good active carbon of preliminary treatment by solid-to-liquid ratio 1:2, and stir frequently, continue 1 hour, vacuum filtration is collected filtrate then;
The preliminary treatment of active carbon: in active carbon, add deionized water by solid-to-liquid ratio 1:3, keep 60 ℃, keep 0.5h, filter then, get filter residue, add 4% watery hydrochloric acid again by solid-to-liquid ratio 1:2, constantly stir and kept 20 minutes, with deionized water it is washed into neutrality then, put into 60 ℃ of oven dry of baking oven afterwards and get final product;
Step 6: filtrate is added in the knockout, add ethyl acetate again and extract, collect the ethyl acetate extraction layer, vacuum is carried out freeze drying after being concentrated into finite concentration then, the freeze drying condition is vacuum 10 Pa, and temperature-50 ℃ obtains cordate houttuynia effective component extracts dry powder.
Embodiment 3: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is pressed following proportioning: houttuynia extract 15 grams, Radix Glycyrrhizae 5 grams, wilsonii 5 grams, vitamin C 0.4 gram, calcium pantothenate 0.6 gram, phenylalanine 0.7 gram, Choline Chloride 0.9 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves and get final product.Wherein, add in the pig feed per ton that 500 these additives of gram mix, granulate, packing gets final product.
Described houttuynia extract obtains in the following manner:
Step 1: gather cordate houttuynia, adopt 60 ℃ of forced air drying modes to carry out drying and handle, dry back uses the plant pulverizer to pulverize, and crosses 40 mesh sieves, gets the powdery cordate houttuynia;
Step 2: with preliminary treatment good the powdery cordate houttuynia is packed in the ultrasonic extractor, add 15 times 80% ethanol, ultrasonic 40 minutes, ultrasonic power was 220W;
Step 3: ultrasonic extraction finishes the back and adopts vacuum filtration, obtains filtrate and filter residue, and filtrate is concentrated into 1/6 of original volume by vacuum, and thickening temperature is 65 ℃, simultaneously ethanol is reclaimed; Filter residue carries out the second time with the mode of step 2 and extracts;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, filtrate is concentrated into 1/6 of original volume by vacuum, thickening temperature is 65 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration with deionized water, 6000 rev/mins of following refrigerated centrifuges 5 minutes, gets supernatant and add the good active carbon of preliminary treatment by solid-to-liquid ratio 1:2, and stir frequently, continue 1.5 hours, vacuum filtration is collected filtrate then;
The preliminary treatment of active carbon: in active carbon, add deionized water by solid-to-liquid ratio 1:4, keep 80 ℃, keep 0.5h, filter then, get filter residue, add 4% watery hydrochloric acid again by solid-to-liquid ratio 1:2, constantly stir and kept 30 minutes, with deionized water it is washed into neutrality then, put into 70 ℃ of oven dry of baking oven afterwards and get final product;
Step 6: filtrate is added in the knockout, add ethyl acetate again and extract, collect the ethyl acetate extraction layer, vacuum is carried out freeze drying after being concentrated into finite concentration then, the freeze drying condition is vacuum 10 Pa, and temperature-60 ℃ obtains cordate houttuynia effective component extracts dry powder.
Embodiment 4: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is pressed following proportioning: houttuynia extract 12.5 grams, Radix Glycyrrhizae 4 grams, wilsonii 4 grams, vitamin C 0.3 gram, calcium pantothenate 0. 45 grams, phenylalanine 0.55 gram, Choline Chloride 0.75 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves and get final product.Wherein, add in the pig feed per ton that 400 these additives of gram mix, granulate, packing gets final product.
Described houttuynia extract obtains in the following manner:
Step 1: gather cordate houttuynia, adopt 90 ℃ of forced air drying modes to carry out drying and handle, dry back uses the plant pulverizer to pulverize, and crosses 40 mesh sieves;
Step 2: the raw material that preliminary treatment is good is packed in the ultrasonic extractor, adds 5 times 40% ethanol, and ultrasonic 15 minutes, ultrasonic power was 100W;
Step 3: ultrasonic extraction finishes the back and adopts vacuum filtration, obtains filtrate and filter residue, and filtrate is concentrated into 1/8 of original volume by vacuum, and thickening temperature is 80 ℃, reclaims ethanol simultaneously; Filter residue carries out the second time with the mode of step 2 and extracts;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, filtrate is concentrated into finite concentration by vacuum, merge for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration with deionized water, 6000 rev/mins of following refrigerated centrifuges 5 minutes, gets supernatant and add the good active carbon of preliminary treatment by solid-to-liquid ratio 1:2, and stir frequently, continue 0.5 hour, vacuum filtration is collected filtrate then;
The preliminary treatment of active carbon: in active carbon, add deionized water by solid-to-liquid ratio 1:2, keep 40 ℃, keep 0.5h, filter then, get filter residue, add 4% watery hydrochloric acid again by solid-to-liquid ratio 1:2, constantly stir and kept 10 minutes, with deionized water it is washed into neutrality then, put into 60 ℃ of oven dry of baking oven afterwards and get final product;
Step 6: filtrate is added in the knockout, add ethyl acetate again and extract, collect the ethyl acetate extraction layer, carry out then carrying out freeze drying after vacuum is concentrated into finite concentration, the freeze drying condition is vacuum 7 Pa, and temperature-35 ℃ obtains cordate houttuynia effective component extracts dry powder.
Embodiment 5: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is pressed following proportioning: houttuynia extract 7 grams, Radix Glycyrrhizae 2 grams, wilsonii 2.5 grams, vitamin C 0.1 gram, calcium pantothenate 0.2 gram, phenylalanine 0.35 gram, Choline Chloride 0.55 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves and get final product.Wherein, add in the pig feed per ton that 200 these additives of gram mix, granulate, packing gets final product.
All the other are with embodiment 1
Embodiment 6: a kind of feed addictive of effective enhancing pig immunity, every hectogram feed addictive is pressed following proportioning: houttuynia extract 7 grams, Radix Glycyrrhizae 2 grams, wilsonii 2.5 grams, vitamin C 0.1 gram, calcium pantothenate 0.2 gram, phenylalanine 0.35 gram, Choline Chloride 0.55 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 35 mesh sieves and get final product.Wherein, add in the pig feed per ton that 200 these additives of gram mix, granulate, packing gets final product.
All the other are with embodiment 4.
Embodiment 7: every hectogram feed addictive is pressed following proportioning: houttuynia extract 12.5 grams, Radix Glycyrrhizae 4 grams, wilsonii 4 grams, vitamin C 0.3 gram, calcium pantothenate 0. 45 grams, phenylalanine 0.55 gram, Choline Chloride 0.75 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.Radix Glycyrrhizae and wilsonii are pulverized respectively, cross 100 mesh sieves and get final product.Wherein, add in the pig feed per ton that 400 these additives of gram mix, granulate, packing gets final product.
Described houttuynia extract extracting method is referring to the extracting method of a Chinese invention patent CN 101085096B cordate houttuynia of Granted publication: adopt conventional extraction method, namely gather cordate houttuynia, adopt the forced air drying mode to carry out drying and handle, dry back uses the plant pulverizer to pulverize, and crosses 40 mesh sieves.Be 70% alcohol extract 3 hour with volumetric concentration with the powder cordate houttuynia under room temperature, stirring condition, the by weight/volume of cordate houttuynia and ethanol is 1:15, filters, and vacuum concentrated frozen drying obtains houttuynia extract.
The test example:
1, experimental animal and experimental design
It is 640 of the health pig of 85.1 ± 2.2kg that Guangnan (Zhanjiang) subordinate plant of tame rich feed corporation,Ltd average weight is selected in test, and it is not remarkable to analyze body weight difference by statistics.Be divided into eight groups at random, every group of 8 repetitions, each repeats 10 male and females half and half, comprising a blank group and 7 test group.Do not add the additive of any enhancing pig immunity that makes among the embodiment 1-7 in the blank group swine rations, add the additive that embodiment 1-7 makes respectively in all the other 7 test group swine rationses, live pig free choice feeding and drinking-water, carry out immunity and expelling parasite routinely, observe and record swine flu situation and pig mental status only every day in experimental period.Totally 30 days experimental period.Weigh during off-test, and ramming material.Record swine flu incidence.Calculate each processed group daily gain, feed intake, feedstuff-meat ratio (feed intake/weightening finish) and the incidence of disease.
Data statistic analysis
Test data adopts the SSPS19.0 statistical software to carry out statistical analysis, the results are shown in following table:
| Project | Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 |
| All the time (kg) | 84.2 | 84.8 | 85.2 | 86.2 | 85.2 | 85 | 84.2 | 85.6 |
| Not heavy (kg) | 108.4 | 111.3 | 112.1 | 112.5 | 110.3 | 110.4 | 109.1 | 110.4 |
| Daily gain (g/d head) | 761 | 892 | 868 | 837 | 828 | 846 | 838 | 806 |
| Daily ingestion amount (g/d head) | 2452 | 2599 | 2622 | 2560 | 2591 | 2578 | 2612 | 2561 |
| Feedstuff-meat ratio | 3.22 | 2.91 | 3.02 | 3.06 | 3.13 | 3.05 | 3.12 | 3.17 |
| The incidence of disease | 12.3% | 1.5% | 2.1% | 1.9% | 5.2% | 4.7% | 6.3% | 7.2% |
Feed intake (ADFI): the feeding capacity of each hurdle test pig of accurate recording and clout amount, calculate every pig average daily ingestion amount and average daily ingestion amount of full phase weekly;
Daily gain (ADG): test pig begins and finish morning 7:30 weekly weighs on an empty stomach, calculates average daily gain and the full phase average daily gain weekly of every pig;
Feedstuff-meat ratio (F/G): calculate according to feed intake and weightening finish.
Disease symptom: sick pig suddenly heating reaches 42 ℃ even higher, dry and hard, the lassitude of ight soil, and anorexia or do not eat, normal accumbency is reluctant activity together, expiratory dyspnea, cough, eye, nose stream mucus.
Conclusion: result of the test shows that the feed addictive basal feed that the present invention makes is compared, and has significantly reduced the incidence of disease of pig, has strengthened the immunity of pig, simultaneously also growth rate, daily gain to a certain extent.By comparing with embodiment 7, the active ingredient that the method for extraction cordate houttuynia active ingredient used in the present invention is extracted than commonsense method is added the better effects if that obtains in the feed to, the incidence of disease of pig is lower, the cordate houttuynia active ingredient purity that extracting method extraction of the present invention is described is higher, more can guarantee the abundant extraction of cordate houttuynia active ingredient.
This products material is the green safety material, and can significantly reduce the incidence of disease of pig, and strengthen the immunity of pig, can be used as antibiotic substitute and add in the pig feed, produce the good mixed feed of disease resisting effect, have good using value and vast market prospect.
Claims (7)
1. feed addictive that effectively strengthens pig immunity, it is characterized in that: every hectogram feed addictive is pressed following proportioning: houttuynia extract 10-15 gram, Radix Glycyrrhizae 3-5 gram, wilsonii 3-5 gram, vitamin C 0.2-0.4 gram, calcium pantothenate 0.3-0.6 gram, phenylalanine 0.4-0.7 gram, Choline Chloride 0.6-0.9 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.
2. according to the described houttuynia extract of claim 1, it is characterized in that its preparation method may further comprise the steps:
Step 1: gather cordate houttuynia, adopt 50-60 ℃ of forced air drying mode to carry out drying and handle, dry back uses the plant pulverizer to pulverize, and crosses 40 mesh sieves, gets the powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that preliminary treatment is good is packed in the ultrasonic extractor, adds the ethanol of 10-15 times of 60%-80%, and ultrasonic 30-40 minute, ultrasonic power was 200-220W;
Step 3: ultrasonic extraction finishes the back and adopts vacuum filtration, obtains filtrate and filter residue, and filtrate is concentrated into the 1/5-1/6 of original volume by vacuum, and thickening temperature is 45-65 ℃, simultaneously ethanol is reclaimed; Filter residue carries out the second time with the mode of step 2 and extracts;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, filtrate is concentrated into the 1/5-1/6 of original volume by vacuum, thickening temperature is 45-65 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration with deionized water, 6000 rev/mins of following refrigerated centrifuges 5 minutes, gets supernatant and add the good active carbon of preliminary treatment by solid-to-liquid ratio 1:2, and stir frequently, continue 1-1.5 hour, vacuum filtration is collected filtrate then;
Step 6: filtrate is added in the knockout, adding ethyl acetate again extracts, collect the ethyl acetate extraction layer, vacuum is carried out freeze drying after being concentrated into finite concentration then, the freeze drying condition is vacuum 10 Pa, temperature-50 ℃ obtains cordate houttuynia effective component extracts dry powder to-60 ℃.
3. according to the preliminary treatment of right 2 described active carbons, it is characterized in that: by solid-to-liquid ratio 1:(3-4) in active carbon, add deionized water, keep 60-80 ℃, keep 0.5h, filter then, get filter residue, again by solid-to-liquid ratio 1:(2-3) add 4% watery hydrochloric acid, constantly stir and kept 20-30 minute, with deionized water it is washed into neutrality then, put into baking oven 60-70 ℃ of oven dry afterwards and get final product.
4. according to the feed addictive of the described a kind of effective enhancing pig immunity of claim 1, every hectogram feed addictive is pressed following proportioning: houttuynia extract 12.5 grams, Radix Glycyrrhizae 4 grams, wilsonii 4 grams, vitamin C 0.3 gram, calcium pantothenate 0.5 gram, phenylalanine 0.4 gram, Choline Chloride 0.7 gram, all the other are carrier, carrier is that wheat bran and defatted rice bran are by mass ratio 1:1.
5. according to the described houttuynia extract of claim 1, it is characterized in that its preparation method may further comprise the steps:
Step 1: gather cordate houttuynia, adopt 55 ℃ of forced air drying modes to carry out drying and handle, dry back uses the plant pulverizer to pulverize, and crosses 40 mesh sieves, gets the powdery cordate houttuynia;
Step 2: the powdery cordate houttuynia that preliminary treatment is good is packed in the ultrasonic extractor, adds 13 times 70% ethanol, and ultrasonic 35 minutes, ultrasonic power was 210W;
Step 3: ultrasonic extraction finishes the back and adopts vacuum filtration, obtains filtrate and filter residue, and filtrate is concentrated into 1/5 of original volume by vacuum, and thickening temperature is 55 ℃, reclaims ethanol simultaneously; Filter residue carries out the second time with the mode of step 2 and extracts;
Step 4: ultrasonic extraction final vacuum suction filtration for the second time, filtrate is concentrated into by vacuum filtrate is concentrated into 1/5 of original volume by vacuum, thickening temperature is 55 ℃, merges for the first time and secondary filtrate;
Step 5: filtrate is diluted to finite concentration with deionized water, 6000 rev/mins of following refrigerated centrifuges 5 minutes, gets supernatant and add the good active carbon of preliminary treatment by solid-to-liquid ratio 1:2, and stir frequently, continue 1.3 hours, vacuum filtration is collected filtrate then;
Step 6: filtrate is added in the knockout, add ethyl acetate again and extract, collect the ethyl acetate extraction layer, carry out then carrying out freeze drying after vacuum is concentrated into finite concentration, the freeze drying condition is vacuum 10 Pa, and temperature-55 ℃ obtains cordate houttuynia effective component extracts dry powder.
6. according to the preliminary treatment of right 2 described active carbons, it is characterized in that: in active carbon, add deionized water by solid-to-liquid ratio 1:4, keep 70 ℃, keep 0.5h, filter then, get filter residue, press the watery hydrochloric acid of solid-to-liquid ratio 1:3 adding 4% again, constantly stir and kept 30 minutes, with deionized water it is washed into neutrality then, put into 70 ℃ of oven dry of baking oven afterwards and get final product.
7. according to the feed addictive of the described a kind of effective enhancing pig immunity of claim 1, the addition in pig feed is the 300-500g/ ton.
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| CN103652492A (en) * | 2013-12-16 | 2014-03-26 | 双胞胎（集团）股份有限公司 | Lactating sow feed additive capable of improving lactation yield and boosting immunity |
| CN106578362A (en) * | 2015-10-17 | 2017-04-26 | 饶平东泽生物科技有限公司 | Feed additive formula |
| CN107667939A (en) * | 2017-11-17 | 2018-02-09 | 金陵科技学院 | Use the method for the bacteriostatic activity of Procambius clarkii checking plant extraction liquid bacterium |
| CN107712347A (en) * | 2017-10-30 | 2018-02-23 | 金华傲农生物科技有限公司 | A kind of additive of compound feed for pig and preparation method and application |
| CN112056470A (en) * | 2020-09-17 | 2020-12-11 | 上海骑骠动物保健品有限公司 | Houttuynia cordata feed additive |
| CN115997961A (en) * | 2022-12-29 | 2023-04-25 | 东莞市吉纯生物技术有限公司 | Granule-loaded nicotine buccal product and preparation method thereof |
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| CN112056470A (en) * | 2020-09-17 | 2020-12-11 | 上海骑骠动物保健品有限公司 | Houttuynia cordata feed additive |
| CN115997961A (en) * | 2022-12-29 | 2023-04-25 | 东莞市吉纯生物技术有限公司 | Granule-loaded nicotine buccal product and preparation method thereof |
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Effective date of registration: 20151228 Address after: 528100 C District, southwest science and Technology Industrial Park, Sanshui District, Guangdong, Foshan, China Patentee after: GUANGDONG TWINS FEED CO., LTD. Address before: 524045 Guangdong Province, Zhanjiang city Chikan North Road No. 2 Patentee before: Guangnan (Zhanjiang) Jiafeng Feed Co.,Ltd. |