CN103314871B - Visual target active attention training system for sober rats/mice - Google Patents
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Abstract
The invention discloses a visual target active attention training system for sober rats/mice. The visual target active attention training system comprises a master control unit, a behavior restricting high table, a visual stimulation display unit, an infrared eye movement monitoring unit, a basic controller, a water feeding rewarding unit and a video monitoring unit. The visual target active attention training system has the advantages that active attention of the tested animals to visual targets can be effectively improved, the conditioned reflex time of the tested rats/mice from generating the attention to the visual targets to behavior feedback is shortened, and the duration of the active attention of the tested rats/mice to the visual targets is prolonged in experimental procedures; the visual target active attention training system supports various visual cognition experimental paradigms, and is easy to extent and high in universality; the visual target active attention training system has important application value and high experimental demands in research fields of coding of neurons of vision systems, brain cognition functions, drug evaluation, plasticity of neural networks and the like.
Description
Technical field
The present invention relates to neuro-physiology and cognitive behavior and learn field, especially relate to greatly clear-headed/mouse sensation target and initiatively pay close attention to training system.
Background technology
Greatly/mouse is the most frequently used model animal in the research of visual system function and visual cognition behavioral function, in experimentation, conventionally all need tested large/mouse can carry out active concern to visual stimulus target.In visual system functional study, how neuron is encoded to characterize to visual stimulus and is had important value, in experiment, need to ensure visual stimulus in the visual field of animal subject in geostationary position.At present for narcose large/the existing very deeply research widely of mouse Neurons In Visual System encoding characteristics, but under waking state, neuron encoding characteristics can be subject to the adjusting of more perceptional factors, there is larger researching value, be therefore subject to showing great attention to of domestic and international researcher.At present, in greatly clear-headed/mouse Neurons In Visual System encoding characteristics research, the main fixing method of animal subject head that adopts, use implanted iron plate that large/mouse head is fixed on stereotaxic instrument, health is forced to be strapped in the fixing constraint cylinder of shape or four limbs are placed on rotatable spheroid.Although these methods can ensure that the head, the visual field of animal, all in geostationary position, easily produce physiology and psychic influence to animal in experimentation, as cause animal struggle, agitation, pain etc.; This experimental state that allows animal " passive " carry out sensation target perception can cause other cortexes to the neururgic complex modulation of visual cortex simultaneously, makes to analyze the Neurons In Visual System visual stimulus encoding characteristics obtaining and has larger bias.
In addition, adopting large/mouse to carry out in the research of visual cognition behavioral function, conventionally utilize certain visual cues to strengthen the specific selection behavior of animal subject, animal subject focuses in plan induction operation behavior (schedule-induced operant) task and plays a key effect the active of sensation target, first needs to train animal subject to carry out active to sensation target and pay close attention in visual cognition behavioral experiment.
At present, to the experimental boxs that adopt narrow space in large/mouse visual cognition behavioral experiment device more, to reduce the interference of animal subject space exploration time in free movement state and other perception factors, but this method is difficult to ensure that animal " sees " in the time that sensation target occurs on the one hand, cause a large amount of invalid examination time, and animal subject is paid close attention to the training time prolongation of screen; On the other hand, while adopting at present large/mouse to carry out the research of visual cognition behavioral experiment, become this field main flow trend in conjunction with implantation micro-electrode having collection analysis nerve signal, but allow animal subject free activity in the experimental box body of narrow space be easy to clashing and breaking electrode, simultaneously, animal subject, from paying close attention to visual cues, making behavior feedback, also longer to the time-histories that finally obtains award, is difficult to determine the state which time window is just carrying out sensation target cognition corresponding to animal subject in nerve signal.
Therefore, need to develop one can either make tested clear-headed large/mouse in experimentation in a kind of nature, can also effectively limit its activity space, being convenient to train animal subject to carry out active to sensation target pays close attention to, shorten the automation training system of Time Created of conditioned reflex between sensation target and behavior response, but yet there are no all reports about this respect.
Summary of the invention
The object of the invention is to provide a kind of large/mouse sensation target of regaining consciousness initiatively to pay close attention to training system, reaching the tested rat of effective raising pays close attention to the active of sensation target, shorten tested rat and set up the time of paying close attention to conditioned reflex between sensation target and behavior feedback, extend initiatively the pay close attention to time of tested rat to sensation target in experimentation.
For achieving the above object, the present invention takes following technical proposals:
Large/mouse the sensation target of regaining consciousness of the present invention is initiatively paid close attention to training system, comprises that main control unit, behavior limit high platform, visual stimulus display unit, infrared eye movement monitoring unit, basic controller, feedwater award unit, video monitoring unit; Described behavior limits high platform and is made up of telescope support and the food dish, the microswitch that are arranged on the upper shed groove at its top and be arranged in described upper shed groove, described behavior limit high platform utilization large/mouse " steep cliff is walked quickly and kept away " effect restriction large/make it in the raw in mouse activity space, effectively reduce the interference of other perception informations; Described visual stimulus display unit is the display screen being disposed on upper shed groove one side stand; Described infrared eye movement monitoring unit is to be arranged on upper shed groove front upper place for monitoring the infrared video watch-dog of rat eyes action, for synchronous recording analyze visual cognition action learning training experiment process tested large/the eye movement state of mouse, whether watch the conditional information of expectation target image attentively for main control unit provides animal subject; Described video monitoring unit is to be arranged on the video monitor of back upper place, training place for training place monitoring; It is the water-storing bottle that is arranged on upper shed groove top that unit is rewarded in described feedwater, and the outlet pipe of described water-storing bottle extends to described food dish position, on described outlet pipe, is provided with electrically-controlled valve, and water supply is rewarded and controlled; Described main control unit video inputs is connected with infrared video watch-dog, video monitor output respectively; Main control unit video output terminals is connected with display screen; Main control unit communication interface is connected with described basic controller, and the image that this main control unit is mainly used to the >30 frame per second that infrared eye movement video monitoring unit is passed back carries out pupil location, eye movement analysis and training data processing; Basic controller input, output interface are connected with described microswitch, electrically-controlled valve drive circuit control end respectively.
The upper shed groove that described behavior limits high platform is deep-slotted chip breaker, described food dish is arranged on one end of upper shed arc groove, described microswitch is arranged on the front of food dish, and upper shed groove adopts insertion slot type structure to be fixed on the supporting plate of high flexible adjustable telescopic support.
The invention has the advantages that the active concern of effective raising animal subject to sensation target, shorten tested large/mouse sets up the time of paying close attention to conditioned reflex between sensation target and behavior feedback, extend in experimentation tested greatly/initiatively the pay close attention to time of mouse to sensation target.The present invention simultaneously supports multiple visual cognition experimental paradigm, is easy to expansion, highly versatile.The present invention has great using value and experiment demand in research fields such as Neurons In Visual System coding, brain cognitive function, assessing drug actions, neutral net plasticity.
Brief description of the drawings
Fig. 1 is structural representation of the present invention.
Fig. 2 is control circuit theory diagram of the present invention.
Embodiment
As shown in Figure 1, 2, large/mouse the sensation target of regaining consciousness of the present invention is initiatively paid close attention to training system, comprises that main control unit 12, behavior limit high platform, visual stimulus display unit, infrared eye movement monitoring unit, basic controller 11, feedwater award unit, video monitoring unit; Described behavior limit high platform utilization large/mouse " steep cliff is walked quickly and kept away " effect restriction large/make it in the raw in mouse activity space, effectively reduce the interference of other perception informations.Described behavior limits high platform by the adjustable telescope support 1 of high flexible and is inserted into the upper shed groove 2 of arcuate structure in its top bracket 13, and is arranged on food dish 3, the microswitch composition of described upper shed groove 2 interior one end; Described microswitch is arranged on the front of food dish 3.Described visual stimulus display unit is the display screen 5 being disposed on upper shed groove 2 one side stands 4; Described infrared eye movement monitoring unit is the infrared video watch-dog 6 that is arranged on upper shed groove 2 tops, for synchronous recording analyze visual cognition action learning training experiment process tested large/the eye movement state of mouse, whether watch the conditional information of expectation target image attentively for main control unit provides animal subject.Described video monitoring unit is the video monitor 7 that is arranged on top, training place, formed by low-light high-definition camera and data acquisition unit, be connected the experimentation playback during for the monitoring of animal subject visual cognition action learning training process or unmanned with main control unit.It is the water-storing bottle 8 that is arranged on upper shed groove 2 tops that unit is rewarded in described feedwater, and the outlet pipe 9 of described water-storing bottle 8 extends to described food and coils 3 positions, is provided with the electrically-controlled valve 10 for supplying water on described outlet pipe 9; The psychological need of unit by using " water deprivation " animal subject to water rewarded in feedwater, reward and adopt electrically-controlled valve feedwater, confluent is easy to control and is not easy to occur executing agency blocks, and feedwater is simultaneously rewarded and can not caused undergo training animal habituation and negative effect, improves accuracy rate and the training speed of training.Described main control unit video inputs is connected with infrared video watch-dog 6, video monitor 7 outputs respectively; Main control unit video output terminals is connected with display screen 5; Main control unit communication interface is connected with described basic controller 11; Main control unit control inputs, output interface are connected with described microswitch, electrically-controlled valve drive circuit control end respectively.
The image (>30 frame per second) that main control unit is mainly used to infrared eye movement video monitoring unit to pass back carries out pupil location, eye movement analysis and training data processing.Data analysis adopts pupil location and trajectory track algorithm, and in the time that eye movement displacement is less than setting threshold, main control unit arranges eye movement status signal for effective, otherwise is invalid.Data analysis is also carried out statistical analysis to the inferior detection data of each experiment examination simultaneously, calculate the index such as behavior response latent period, touching accuracy, when tested reaching when training requirement maybe cannot complete training gives experimenter voice message, automatically complete whole training process.
Training method of the present invention is summarized as follows:
Animal subject is initiatively paid close attention to and is trained the incremental following three phases that is divided into sensation target, and training goal, training mode and the award controlled condition in each stage arrange as follows:
First stage: reward training
Training goal: tested large/mouse adapts to high platform and do not fall, and association touches microswitch and obtains feedwater and reward.
Training mode:
1, the high platform environment of tested adaptation, ensures not fall, and this stage visual stimulus display unit is without visual stimulus and the encouraging pattern that uniformly-spaced feeds water;
2, tested control water is placed on behavior and limits on high platform, and the complete grey screen display of this stage visual stimulus display unit is shown, applying feedwater and rewarding in the time triggering microswitch.
Reward controlled condition: trigger microswitch and think that behavior response is correct, award;
Second stage: visual attention location reflection is set up
Training goal: set up the conditioned reflex of touching microswitch behavior in the time there is image on display screen.
Training mode: visual stimulus is for examination time presents image after starting, exceeding image stimulates and presents display screen ash screen display after duration and show.
Reward controlled condition: whole examination time interior touchless microswitch behavior is considered as invalid examination time.Effectively in examination time, within the examination time cycle image present during synchronous recording analyze tested eye movement state, award if touch microswitch after paying close attention to screen in the display image period and switching to the ash screen period.
Claims (2)
1. greatly clear-headed/mouse sensation target is initiatively paid close attention to a training system, be it is characterized in that: comprise that main control unit (12), behavior limit high platform, visual stimulus display unit, infrared eye movement monitoring unit, basic controller (11), feedwater award unit, video monitoring unit; Described behavior limits high platform by telescope support (1) and is arranged on the upper shed groove (2) at its top and is arranged on described upper shed groove (2) interior food dish (3), microswitch and forms, described behavior limit high platform utilization large/mouse " steep cliff is walked quickly and kept away " effect restriction large/make it in the raw in mouse activity space, effectively reduce the interference of other perception informations; Described visual stimulus display unit is for being disposed on the display screen (5) on upper shed groove (2) one side stands (4); Described infrared eye movement monitoring unit is for being arranged on upper shed groove (2) front upper place for monitoring the infrared video watch-dog (6) of rat eyes action, for synchronous recording analyze visual cognition action learning training experiment process tested large/the eye movement state of mouse, whether watch the conditional information of expectation target image attentively for main control unit provides animal subject; Described video monitoring unit is to be arranged on the video monitor (7) of back upper place, training place for training place monitoring; Described feedwater is rewarded unit for being arranged on the water-storing bottle (8) above upper shed groove (2), the outlet pipe (9) of described water-storing bottle (8) extends to described food dish (3) position, on described outlet pipe (9), be provided with electrically-controlled valve (10), water supply is rewarded and is controlled; Described main control unit video inputs is connected with infrared video watch-dog (6), video monitor (7) output respectively; Main control unit video output terminals is connected with display screen (5); Main control unit communication interface is connected with described basic controller (11), and the image that this main control unit is mainly used to the >30 frame per second that infrared eye movement video monitoring unit is passed back carries out pupil location, eye movement analysis and training data processing; Basic controller (11) input, output interface are connected with described microswitch, electrically-controlled valve drive circuit control end respectively.
2. large/mouse the sensation target of regaining consciousness according to claim 1 is initiatively paid close attention to training system, it is characterized in that: the upper shed groove (2) that described behavior limits high platform is deep-slotted chip breaker, described food dish (3) is arranged on one end of upper shed arc groove, described microswitch is arranged on the front of food dish (3), and upper shed groove (2) adopts insertion slot type structure to be fixed on the supporting plate (13) of adjusting telescope support (1).
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| CN106508714B (en) * | 2016-10-25 | 2019-04-05 | 郑州大学 | Animal material object target identification learning training system |
| CN106719082B (en) * | 2016-12-28 | 2018-12-14 | 华东师范大学 | A kind of experimental provision detecting small birds cognitive function |
| CN107041322A (en) * | 2017-05-02 | 2017-08-15 | 中国人民解放军军事医学科学院基础医学研究所 | Non-human primates vision and Cognitive Function Research system |
| CN107411762A (en) * | 2017-08-31 | 2017-12-01 | 杭州极智医疗科技有限公司 | Cognitive disorder diagnostic equipment |
| CN109011655A (en) * | 2018-08-18 | 2018-12-18 | 胡绍富 | A kind of flying squirrel gliding performance system |
| CN111024934B (en) * | 2019-12-27 | 2021-12-07 | 苏州溢博伦光电仪器有限公司 | Vibration reduction type microscopic observation method |
| CN113796335B (en) * | 2021-08-26 | 2023-03-24 | 五邑大学 | Control method, device, equipment and storage medium of mouse behavior training system |
| CN115226648B (en) * | 2022-07-18 | 2023-09-22 | 陕西师范大学 | Small-sized automatic animal behavior training system and training method |
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| CN103125406A (en) * | 2013-03-19 | 2013-06-05 | 郑州大学 | Visual cognitive behavioral learning automatic training system of big and small mice |
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| CN103026973A (en) * | 2012-11-30 | 2013-04-10 | 中国航天员科研训练中心 | Device for detecting, analyzing and processing reward operation condition reflection in real time |
| CN103109755A (en) * | 2013-02-21 | 2013-05-22 | 河海大学常州校区 | Device and method for controlling animal vision and tracking animal motions in natural environment |
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