CN103308346A - Method for researching tobacco roots and nutrient distribution of rhizosphere soil, and rhizosphere soil excavator - Google Patents
Method for researching tobacco roots and nutrient distribution of rhizosphere soil, and rhizosphere soil excavator Download PDFInfo
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- CN103308346A CN103308346A CN2013102762823A CN201310276282A CN103308346A CN 103308346 A CN103308346 A CN 103308346A CN 2013102762823 A CN2013102762823 A CN 2013102762823A CN 201310276282 A CN201310276282 A CN 201310276282A CN 103308346 A CN103308346 A CN 103308346A
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Abstract
The invention discloses a method for researching tobacco roots and the nutrient distribution of rhizosphere soil, and a rhizosphere soil excavator. The method comprises the following steps of: a, drawing a rectangular block at the periphery of a tobacco plant according to an actual area occupied by each tobacco strain in a large field; b, deeply digging 40-50 cm downwards by taking the rectangular block as a base line, and forming a cubic soil block at the bottom of the tobacco strain; d, slicing the cubic soil block by the rhizosphere soil excavator, respectively bagging all visible roots and soil after the cubic soil block is sliced, and marking numbers on the bags by a three-dimensional coordinate method; and e, bringing the bagged roots and the bagged soil which are subjected to number marking back to a laboratory for scanning the roots and measuring the nutrients of the soil. The method has the advantages that the total number of excavated roots is large; the operation is simple and labor-saving; the excavated soil blocks are standard; the roots and the rhizosphere soil can be simultaneously obtained; researches on the roots and the transverse and longitudinal distributions of the soil nutrients are sufficient; the rhizosphere soil excavator is made of a metal material and is simple in structure, easy to learn, durable and used safely.
Description
Technical field
The present invention relates to a kind of method and Rhizosphere Soil delver of studying tobacco root and Rhizosphere soil nutrient distribution, belong to root system of plant and Distribution of Nutrient studying technological domain.
Background technology
At present, the Main Means of flue-cured tobacco root system research comprises that indoor nutrient solution is cultivated, silica sand is cultivated, the root case is tested and the whole strain in field takes method.Wherein, indoor nutrient solution is cultivated and the silica sand cultivation can be obtained comparatively complete root system, yet differs too greatly and usually Root growth is caused the restriction in space with the growing way of actual field plant root system; The test of root case or earth culture test, relatively near the growing way situation of actual field plant root system, still, the root system that obtains is about about 60% of actual root system.Field integral body takes the actual conditions that method can be reacted root growth, yet the ratio that the root system that the mode that integral body takes is obtained accounts for total root system is lower, and can not react the steric configuration of root system.
The research of Rhizosphere soil nutrient, at present main is to adopt soil profile and soil drilling method.Wherein, soil profile take the side soil nutrient that can only study root system, if require great effort during the telephone expenses that section is dug at the four sides, and can't study migration and the Changing Pattern of Rhizosphere soil nutrient; Soil drilling method can comparatively be saved time, but comprehensive not to the research of rhizosphere soil distribution and Changing Pattern.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of method and Rhizosphere Soil delver of studying tobacco root and Rhizosphere soil nutrient distribution, it provides a kind of brand-new thinking and more careful and comprehensive research means, utilize root system and Rhizosphere Soil delver, the way that takes in conjunction with three-dimensional piecemeal is to the research of field root system configuration and the horizontal and vertical distribution of Rhizosphere Soil nutrient and mutual relationship thereof more comprehensively and fully; Utilize the Rhizosphere Soil delver can take more accurately root system and Rhizosphere Soil, standard and laborsaving can overcome the deficiencies in the prior art more.
The technical scheme of patent of the present invention is: the Rhizosphere Soil delver that research tobacco root and Rhizosphere soil nutrient distribute, it inserts steel disc by the dustpan-shaped excavation steel box that is used in conjunction with each other and bottom and consists of, the structure of dustpan-shaped excavation steel box is made of rectangular bottom surface and the side plate that is connected to rectangular bottom surface three limits, is connected with hand holding handle in rectangular bottom surface; The bottom insert steel disc be area greater than the metal featheredge of rectangular bottom surface, be connected with handle at sheet metal.
A kind of method of studying tobacco root and Rhizosphere soil nutrient distribution may further comprise the steps:
A, calculate the shared real area of every strain cigarette strain according to land for growing field crops tobacco planting densitometer, mark rectangular box in cigarette strain periphery subsequently;
B, take rectangular box as baseline, deep-cut 40-50cm downwards, form the cube soil block in cigarette strain bottom;
D, utilize the Rhizosphere Soil delver that the cube soil block is carried out stripping and slicing, all root systems that can see and soil after the stripping and slicing are packed respectively, and adopt the mode of three-dimensional coordinate to carry out label;
E, the root system after the label pack and soil taken back root system scanning is carried out in the laboratory and soil nutrient is measured.
In the method that above-mentioned research tobacco root and Rhizosphere soil nutrient distribute, rectangular box is according to vega area and planting density, calculates the real area that each strain cigarette strain takies, and then, marks length and width according to line-spacing and spacing in the rows.
In the method that aforesaid research tobacco root and Rhizosphere soil nutrient distribute, utilize the Rhizosphere Soil delver to the method that the cube soil block carries out stripping and slicing to be: to insert dustpan-shaped excavation steel box in the soil body downwards, lateral notch facing to the cube soil block without native side, the soil body that soil density is large can assist rubber mallet to knock downwards, then the soil body until dustpan-shaped excavation steel box submerges fully inserts the side plate lower surface insertion soil body formation stripping and slicing that steel disc pastes dustpan-shaped excavation steel box with the bottom.
Compared with the prior art, the present invention carries out stripping and slicing with the Rhizosphere Soil delver to the cube soil block, all root systems that can see and soil after the stripping and slicing is packed respectively, and adopt the mode of three-dimensional coordinate to carry out label; Take back the laboratory behind the label and carry out root system scanning and soil nutrient mensuration.Its method provides a kind of brand-new thinking and more careful and comprehensive research means, so that more comprehensive and abundant to the research of field root system configuration and the horizontal and vertical distribution of Rhizosphere Soil nutrient and mutual relationship thereof; Utilize the Rhizosphere Soil delver can take more accurately root system and Rhizosphere Soil, more standard and laborsaving.It has the following advantages: the root system total amount that excavate out (1) is large; (2) soil block standard simple to operate, laborsaving, as to take; (3) can obtain simultaneously root system and Rhizosphere Soil, more abundant to the research of the horizontal and vertical distribution of root system and Rhizosphere soil nutrient; (4) adopt metal material to make, durable, use safety simple in structure, easy to learn.
Description of drawings
Fig. 1 is the structural representation of dustpan-shaped excavation steel box;
Fig. 2 is the structural representation that steel disc is inserted in the bottom.
Embodiment
The structure of Rhizosphere Soil delver is by the dustpan-shaped excavation steel box 1(that is used in conjunction with each other as shown in Figure 1) and bottom insertion steel disc 2(is as shown in Figure 2) consist of, the structure of dustpan-shaped excavation steel box 1 is made of rectangular bottom surface and the side plate that is connected to rectangular bottom surface three limits, is connected with hand holding handle 3 in rectangular bottom surface; The bottom insert steel disc 2 for area greater than the metal featheredge that is equivalent to rectangular bottom surface, be connected with handle 4 at sheet metal.
A kind of method of studying tobacco root and Rhizosphere soil nutrient distribution may further comprise the steps:
A, mark rectangular box according to the land for growing field crops shared area of every strain cigarette strain in cigarette strain periphery; Rectangular box is according to vega area and planting density, calculates the real area that each strain cigarette strain takies, and then, marks length and width according to line-spacing and spacing in the rows;
B, take rectangular box as baseline, deep-cut 40-50cm downwards, form the cube soil block in cigarette strain bottom;
D, utilize the Rhizosphere Soil delver that the cube soil block is carried out stripping and slicing, all root systems that can see and soil after the stripping and slicing are packed respectively, and adopt the mode of three-dimensional coordinate to carry out label; Utilize the Rhizosphere Soil delver to the method that the cube soil block carries out stripping and slicing to be: to insert dustpan-shaped excavation steel box in the soil body downwards, lateral notch facing to the cube soil block without native side, the soil body that soil density is large can assist rubber mallet to knock downwards, the soil body until dustpan-shaped excavation steel box submerges fully, then insert the side plate lower surface insertion soil body formation stripping and slicing that steel disc pastes dustpan-shaped excavation steel box with the bottom, the size of dustpan-shaped excavation steel box (1) adopts 10 * 10 * 10cm, therefore obtaining stripping and slicing is 10 * 10 * 10cm cube soil block, subsequently root system and soil in the cube soil block are separately deposited, with 3 D stereo coordinate mark.
E, the root system after the label pack and soil taken back root system scanning is carried out in the laboratory and soil nutrient is measured.
Claims (4)
1. study the Rhizosphere Soil delver that tobacco root and Rhizosphere soil nutrient distribute for one kind, it is characterized in that: it inserts steel disc (2) by the dustpan-shaped excavation steel box (1) that is used in conjunction with each other and bottom and consists of, the structure of dustpan-shaped excavation steel box (1) is made of rectangular bottom surface and the side plate that is connected to rectangular bottom surface three limits, is connected with hand holding handle (3) in rectangular bottom surface; The bottom insert steel disc (2) for area greater than the metal featheredge that is equivalent to rectangular bottom surface, be connected with handle (4) at sheet metal.
2. a kind of method that tobacco root and Rhizosphere soil nutrient distribute of studying according to claim 1, it is characterized in that: the method may further comprise the steps:
A, mark rectangular box according to the land for growing field crops shared real area of every strain cigarette strain in cigarette strain periphery;
B, take rectangular box as baseline, deep-cut 40-50cm downwards, form the cube soil block in cigarette strain bottom;
D, utilize the Rhizosphere Soil delver that the cube soil block is carried out stripping and slicing, all root systems that can see and soil after the stripping and slicing are packed respectively, and adopt the mode of three-dimensional coordinate to carry out label;
E, the root system after the label pack and soil taken back root system scanning is carried out in the laboratory and soil nutrient is measured.
3. the method that distributes of research tobacco root according to claim 2 and Rhizosphere soil nutrient, it is characterized in that: rectangular box is according to vega area and planting density, calculate the real area that each strain cigarette strain takies, then, mark length and width according to line-spacing and spacing in the rows.
4. the method that distributes of research tobacco root according to claim 2 and Rhizosphere soil nutrient, it is characterized in that: utilizing the Rhizosphere Soil delver is to insert dustpan-shaped excavation steel box in the soil body downwards to the method that the cube soil block carries out stripping and slicing, lateral notch facing to the cube soil block without native side, the soil body that soil density is large can assist rubber mallet to knock downwards, then the soil body until dustpan-shaped excavation steel box submerges fully inserts the side plate lower surface insertion soil body formation stripping and slicing that steel disc pastes dustpan-shaped excavation steel box with the bottom.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105606729A (en) * | 2014-12-26 | 2016-05-25 | 东北农业大学 | Detection method for simulating transverse distribution of herbicide in soil layer |
CN112986058A (en) * | 2021-02-02 | 2021-06-18 | 贵州省烟草科学研究院 | Visual analysis method for migration rule of fertilizer nutrients in soil |
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CN2864656Y (en) * | 2005-12-19 | 2007-01-31 | 祖述冲 | Soil sample collector |
CN101881705A (en) * | 2010-06-24 | 2010-11-10 | 河南科技大学 | Combined quantitative sampler used for researching plant root distribution and root morphology |
CN101923011A (en) * | 2010-06-29 | 2010-12-22 | 中国科学院新疆生态与地理研究所 | Three-dimensional sampling method applicable to root system research |
CN202453212U (en) * | 2011-12-09 | 2012-09-26 | 农业部环境保护科研监测所 | Portable undisturbed soil sampler |
CN203310648U (en) * | 2013-07-04 | 2013-11-27 | 贵州省烟草科学研究院 | Rhizosphere soil excavator used for studying tobacco root and nutrient distribution of rhizosphere soil |
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2013
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2864656Y (en) * | 2005-12-19 | 2007-01-31 | 祖述冲 | Soil sample collector |
CN101881705A (en) * | 2010-06-24 | 2010-11-10 | 河南科技大学 | Combined quantitative sampler used for researching plant root distribution and root morphology |
CN101923011A (en) * | 2010-06-29 | 2010-12-22 | 中国科学院新疆生态与地理研究所 | Three-dimensional sampling method applicable to root system research |
CN202453212U (en) * | 2011-12-09 | 2012-09-26 | 农业部环境保护科研监测所 | Portable undisturbed soil sampler |
CN203310648U (en) * | 2013-07-04 | 2013-11-27 | 贵州省烟草科学研究院 | Rhizosphere soil excavator used for studying tobacco root and nutrient distribution of rhizosphere soil |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105606729A (en) * | 2014-12-26 | 2016-05-25 | 东北农业大学 | Detection method for simulating transverse distribution of herbicide in soil layer |
CN105606729B (en) * | 2014-12-26 | 2018-07-20 | 东北农业大学 | A kind of detection method of simulation herbicide cross direction profiles in soil layer |
CN112986058A (en) * | 2021-02-02 | 2021-06-18 | 贵州省烟草科学研究院 | Visual analysis method for migration rule of fertilizer nutrients in soil |
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Application publication date: 20130918 |