CN103300231A - Protein feed special for laying duck as well as enzymolysis and fermentation co-production method thereof - Google Patents
Protein feed special for laying duck as well as enzymolysis and fermentation co-production method thereof Download PDFInfo
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- CN103300231A CN103300231A CN 201210066057 CN201210066057A CN103300231A CN 103300231 A CN103300231 A CN 103300231A CN 201210066057 CN201210066057 CN 201210066057 CN 201210066057 A CN201210066057 A CN 201210066057A CN 103300231 A CN103300231 A CN 103300231A
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Abstract
The invention discloses a protein feed special for a laying duck as well as an enzymolysis and fermentation co-production method thereof. The method comprises the following steps: (1) adding an enzymolysis enzyme and water into a raw material composition; performing enzymolysis; collecting a liquid phase after the enzymolysis; removing water in a concentrating manner and drying, thereby obtaining a solid enzymolysis product; (2) by taking the obtained solid enzymolysis product as a substrate, adding strains obtained after being propagated in an expanding manner and performing a solid fermentation; (3) drying and crushing a fermentation product, thereby obtaining the protein feed. The protein feed is high in protein content, uniform in AA proportion and high in small peptide content, wherein the content of an acid soluble protein is 50.02%; the peptide content is 48.25%; the molecular weight is less than 95.74% of 5000dolton. The protein feed is rich in peptides, antibacterial peptides, immune peptides and the like with special functions, and has obvious effects for promoting the digestive absorption of the laying duck, balancing the nutrition of the laying duck and enhancing the immunity of the laying duck. The protein feed can be used for replacing fish meal and is superior to the fish meal when being applied to the laying duck.
Description
Technical field
The present invention relates to a kind of production method of protein feed, belong to field of feed processing.
Background technology
European Union completely forbids from January, 2006 and add antibiotic in animal and fowl fodder, and must not add antibiotic in raising has been internationally recognized food security standard, and the research that utilizes the fermented feed containing probiotics raw material to produce the multicomponent Functional Polypeptides has also obtained a little progress.The viewpoint that scientific circles have tended to admit is following consideration development alternative medicine or finds that antimicrobial natural materials is lasting to find, effectively controls the new way of pathogenicity disease.
Probiotic is of a great variety, FDA and AAFCO have announced kind more than 340, have 16 kinds in No. 1126 bulletins that our Ministry of Agriculture announced in 2008, but owing to the selection to bacterial classification is used in conjunction with difference, the control that adds technique is different, and the product cost performance that is finally shown also has difference.
Present egg duck special protein feed, protein content is low, and the AA ratio is unbalanced, and little peptide content is low, is unfavorable for promoting digesting and assimilating of egg duck, can not satisfy the demand.
Existing market setting egg(s) duck mainly is fish meal and dregs of beans with protein raw materials, and its main defective is that amino acid is unbalanced, and is expensive, does not have anti-microbial property fully simultaneously, and its cost performance is lower.
Summary of the invention
The object of the invention is to provide the method for a kind of egg duck special protein feed and enzymolysis and fermentation in combining production, the defects that exists to overcome prior art.
Enzymolysis of the present invention and fermentation in combining are given birth to the method for laying ducks special protein feed, comprise the steps:
(1) enzymolysis enzyme and water are added raw material, carry out enzymolysis, the liquid phase behind the collection enzymolysis, behind the concentrated removal moisture content, drying obtains the enzymolysis solid product;
The percentage by weight of each component is:
Surplus is water.
Preferably, described cotton dregs are the high protein cotton dregs of protein content more than 48% that produce in Xinjiang;
Described enzymolysis enzyme is selected from papain 2*10
5U/G, neutral proteinase 2*10
5U/G, Aspergillus proteinase 3*10
5U/G or phytase 5000U/G, can adopt the commercially available prod, such as the papain of Nanning Jie Woli biological products Co., Ltd, the neutral proteinase of Shandong Anke Bioengineering Co., Ltd., the Aspergillus proteinase of GuangZhou KeRen Biology Engineering Co., Ltd, such as the overflow phytase of company of how sharp bioengineering Co., Ltd of Guangdong;
Above-mentioned " U/G " refers to the enzyme activity that every milligram of zymoprotein has;
Hydrolysis temperature is 30～80 ℃, and enzymolysis time is 20～60 hours;
Baking temperature is 0-30 ℃, and be 2～20 seconds drying time;
(2) then take the enzymolysis solid product that obtained as substrate, add the bacterial classification that obtains after the expanding propagation, carry out solid fermentation;
Described bacterial classification is selected from Candida tropicalis, aspergillus oryzae or withered grass bud moon born of the same parents bacillus KD-N2;
Above-mentioned bacterial classification can adopt business-like product, give birth to the product of friendship bio tech ltd company such as Shanghai, also can buy by Chinese microbial preservation center, so long as the bacterial classification of Candida tropicalis, aspergillus oryzae or withered grass bud moon born of the same parents bacillus class all can be used in the present invention;
During solid fermentation, add entry, take the gross weight of fermentation system as benchmark, the weight content of moisture content is 30-50%;
The method of bacterial classification expanding propagation is conventional, as comprises the steps:
Original seed is after slant medium is cultivated, and expanding propagation successively in little triangular flask, large triangular flask enters the 500L seeding tank and makes the one-level kind, after enter 6000L seeding tank make the secondary kind, in the seeding tank of 2000L, make three grades of kinds;
The bacterial classification incubation is liquid fermentation, and the nutrient solution that each step is cultivated bacterial classification all enters in next step culture vessel together with bacterial classification, does not have waste liquid to produce;
The parts by weight of the culture medium of training through the inclined-plane are:
The parts by weight of the culture medium of little triangular flask, large triangular flask are:
The parts by weight of the culture medium of one-level kind, secondary kind and three grades of kinds are:
(3) tunning is dried successively, pulverized, obtain product;
Preferably, by pulverizing after the vulcanization bed vacuum drying, the weight content of water is less than 10%.
The invention has the beneficial effects as follows: the present invention takes enzymolysis and fermentation in combining method to give birth to laying ducks special protein feed, get the length of two kinds of methods, product has higher protein content, the AA ratio is more balanced, little peptide content is high, the molten albumen of acid (in butt) 50.02%, peptide content (in butt) 48.25%, molecular weight accounts for 95.74% less than the peptide of 5000dolton, be rich in simultaneously function special peptide class such as somatomedin, antibacterial peptide, immune peptide etc., to promoting digesting and assimilating of egg duck, nutrient balance, strengthen immunity and have significant effect, being used for the egg duck can Peru Fish Dietary, and is better than fish meal.
Term " AA ratio " refers to amino acid ratio.
The specific embodiment
Embodiment 1
(1) enzymolysis enzyme and water are added raw material, carry out enzymolysis, the liquid phase behind the collection enzymolysis, behind the concentrated removal moisture content, drying obtains the enzymolysis solid product;
The percentage by weight of each component is:
Surplus is water.
Described cotton dregs are the high protein cotton dregs of the protein content 48.5% of Xinjiang product;
Described enzymolysis enzyme is papain 2*10
5U/G adopts the papain of Nanning Jie Woli biological products Co., Ltd;
Hydrolysis temperature is 80 ℃, and enzymolysis time is 20 hours;
Baking temperature is 30 ℃, and be 2 seconds drying time;
(2) then take the enzymolysis solid product that obtained as substrate, add the bacterial classification that obtains after the expanding propagation, carry out solid fermentation;
Bacterial classification is Candida tropicalis;
During solid fermentation, add entry, take the gross weight of fermentation system as benchmark, the weight content of moisture content is 30%;
The method of bacterial classification expanding propagation comprises the steps:
Original seed is after slant medium is cultivated, and expanding propagation successively in little triangular flask, large triangular flask enters the 500L seeding tank and makes the one-level kind, after enter 6000L seeding tank make the secondary kind, in the seeding tank of 2000L, make three grades of kinds;
The bacterial classification incubation is liquid fermentation, and the nutrient solution that each step is cultivated bacterial classification all enters in next step culture vessel together with bacterial classification, does not have waste liquid to produce;
The parts by weight of the culture medium of training through the inclined-plane are:
The parts by weight of the culture medium of little triangular flask, large triangular flask are:
The parts by weight of the culture medium of one-level kind, secondary kind and three grades of kinds are:
(3) tunning is pulverized after by the vulcanization bed vacuum drying, obtained product, the weight content of water is less than 10%.
Adopt the method for GB regulation, detect, product has higher protein content, little peptide content is 50.2%, and sour molten albumen (take butt) is 51.02%, and molecular weight is less than 98.74% (weight) that accounts for of 5000dolton, be rich in simultaneously the special peptide class of function such as somatomedin, antibacterial peptide, immune peptide etc., to promoting the digesting and assimilating of egg duck, nutrient balance, enhancing immunity to have significant effect, being used for the egg duck can Peru Fish Dietary, and is better than fish meal.
Embodiment 2
(1) enzymolysis enzyme and water are added raw material, carry out enzymolysis, the liquid phase behind the collection enzymolysis, behind the concentrated removal moisture content, drying obtains the enzymolysis solid product;
The percentage by weight of each component is:
Surplus is water.
Described cotton dregs are the high protein cotton dregs of the protein content 48.5% of Xinjiang product;
Described enzymolysis enzyme is neutral proteinase 2*10
5U/G adopts the neutral proteinase of Shandong Anke Bioengineering Co., Ltd.;
Hydrolysis temperature is 30 ℃, and enzymolysis time is 60 hours;
Baking temperature is 0 ℃, and be 20 seconds drying time;
(2) then take the enzymolysis solid product that obtained as substrate, add the bacterial classification that obtains after the expanding propagation, carry out solid fermentation;
Bacterial classification is aspergillus oryzae;
During solid fermentation, add entry, take the gross weight of fermentation system as benchmark, the weight content of moisture content is 50%;
The method of bacterial classification expanding propagation comprises the steps:
Original seed is after slant medium is cultivated, and expanding propagation successively in little triangular flask, large triangular flask enters the 500L seeding tank and makes the one-level kind, after enter 6000L seeding tank make the secondary kind, in the seeding tank of 2000L, make three grades of kinds;
The bacterial classification incubation is liquid fermentation, and the nutrient solution that each step is cultivated bacterial classification all enters in next step culture vessel together with bacterial classification, does not have waste liquid to produce;
The parts by weight of the culture medium of training through the inclined-plane are:
The parts by weight of the culture medium of little triangular flask, large triangular flask are:
The parts by weight of the culture medium of one-level kind, secondary kind and three grades of kinds are:
(3) tunning is pulverized after by the vulcanization bed vacuum drying, obtained product, the weight content of water is less than 10%.
Adopt the method for GB regulation, detect, product has higher protein content, and little peptide content is acid molten 49.8%, the molten albumen of acid (take butt) is 50.02%, molecular weight is rich in the special peptide class of function such as somatomedin, antibacterial peptide, immune peptide etc. simultaneously less than 96.74% (weight) that accounts for of 5000dolton, and has significant effect to promoting the digesting and assimilating of egg duck, nutrient balance, enhancing immunity, being used for the egg duck can Peru Fish Dietary, and is better than fish meal.
Embodiment 3
(1) enzymolysis enzyme and water are added raw material, carry out enzymolysis, the liquid phase behind the collection enzymolysis, behind the concentrated removal moisture content, drying obtains the enzymolysis solid product;
The percentage by weight of each component is:
Surplus is water.
Described cotton dregs are the high protein cotton dregs of the protein content 48% of Xinjiang product;
Described enzymolysis enzyme is the Aspergillus proteinase of GuangZhou KeRen Biology Engineering Co., Ltd;
Hydrolysis temperature is 30 ℃, and enzymolysis time is 60 hours;
Baking temperature is 0 ℃, and be 20 seconds drying time;
(2) then take the enzymolysis solid product that obtained as substrate, add the bacterial classification that obtains after the expanding propagation, carry out solid fermentation;
Bacterial classification is withered grass bud moon born of the same parents bacillus KD-N2;
During solid fermentation, add entry, take the gross weight of fermentation system as benchmark, the weight content of moisture content is 50%;
The method of bacterial classification expanding propagation comprises the steps:
Original seed is after slant medium is cultivated, and expanding propagation successively in little triangular flask, large triangular flask enters the 500L seeding tank and makes the one-level kind, after enter 6000L seeding tank make the secondary kind, in the seeding tank of 2000L, make three grades of kinds;
The bacterial classification incubation is liquid fermentation, and the nutrient solution that each step is cultivated bacterial classification all enters in next step culture vessel together with bacterial classification, does not have waste liquid to produce;
The parts by weight of the culture medium of training through the inclined-plane are:
The parts by weight of the culture medium of little triangular flask, large triangular flask are:
The parts by weight of the culture medium of one-level kind, secondary kind and three grades of kinds are:
(3) tunning is pulverized after by the vulcanization bed vacuum drying, obtained product, the weight content of water is less than 10%.
Adopt the method for GB regulation, detect, product has higher protein content, and little peptide content is, acid molten 50.8%, the molten albumen of acid (take butt) is 49.56%, and molecular weight is less than 97.74% (weight) that accounts for of 5000dolton, be rich in simultaneously the special peptide class of function such as somatomedin, antibacterial peptide, immune peptide etc., to promoting the digesting and assimilating of egg duck, nutrient balance, enhancing immunity to have significant effect, being used for the egg duck can Peru Fish Dietary, and is better than fish meal.
Claims (10)
1. the method for egg duck special protein feed enzymolysis and fermentation in combining production is characterized in that, comprises the steps:
(1) enzymolysis enzyme and water are added raw material, carry out enzymolysis, the liquid phase behind the collection enzymolysis, behind the concentrated removal moisture content, drying obtains the enzymolysis solid product;
(2) then take the enzymolysis solid product that obtained as substrate, add the bacterial classification that obtains after the expanding propagation, carry out solid fermentation;
(3) tunning is dried successively, pulverized, obtain product.
2. method according to claim 1 is characterized in that, by pulverizing after the vulcanization bed vacuum drying, the weight content of water is less than 10%.
3. method according to claim 1 is characterized in that, in the step (1), the percentage by weight of each component is:
Surplus is water.
4. method according to claim 3 is characterized in that, described cotton dregs are the high protein cotton dregs of protein content more than 48% that produce in Xinjiang.
5. method according to claim 1 is characterized in that, described enzymolysis enzyme is selected from papain 2*10
5U/G, neutral proteinase 2*10
5U/G, Aspergillus proteinase 3*10
5U/G or phytase 5000U/G.
6. method according to claim 1 is characterized in that, in the step (1), hydrolysis temperature is 30～80 ℃, and enzymolysis time is 20～60 hours; Baking temperature is 0-30 ℃, and be 2～20 seconds drying time.
7. method according to claim 1 is characterized in that, in the step (2), described bacterial classification is selected from Candida tropicalis, aspergillus oryzae or withered grass bud moon born of the same parents bacillus KD-N2.
8. method according to claim 1 is characterized in that, during solid fermentation, adds entry, and take the gross weight of fermentation system as benchmark, the weight content of moisture content is 30-50%.
9. each described method is characterized in that according to claim 1～8, and the method for bacterial classification expanding propagation is, comprises the steps:
Original seed is after slant medium is cultivated, and expanding propagation successively in little triangular flask, large triangular flask enters the 500L seeding tank and makes the one-level kind, after enter 6000L seeding tank make the secondary kind, in the seeding tank of 2000L, make three grades of kinds;
The bacterial classification incubation is liquid fermentation, and the nutrient solution that each step is cultivated bacterial classification all enters in next step culture vessel together with bacterial classification;
The parts by weight of the culture medium of training through the inclined-plane are:
The parts by weight of the culture medium of little triangular flask, large triangular flask are:
The parts by weight of the culture medium of one-level kind, secondary kind and three grades of kinds are:
10. the egg duck special protein feed of each described method preparation according to claim 1～9.
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| CN 201210066057 CN103300231A (en) | 2012-03-14 | 2012-03-14 | Protein feed special for laying duck as well as enzymolysis and fermentation co-production method thereof |
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| CN 201210066057 CN103300231A (en) | 2012-03-14 | 2012-03-14 | Protein feed special for laying duck as well as enzymolysis and fermentation co-production method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104106739A (en) * | 2014-06-19 | 2014-10-22 | 安徽强英鸭业集团有限公司 | High protein feed for laying ducks |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104106739A (en) * | 2014-06-19 | 2014-10-22 | 安徽强英鸭业集团有限公司 | High protein feed for laying ducks |
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Application publication date: 20130918 |