CN103300073B - Cannabis sativa antibacterial liquid as well as preparation method and application of cannabis sativa antibacterial liquid - Google Patents
Cannabis sativa antibacterial liquid as well as preparation method and application of cannabis sativa antibacterial liquid Download PDFInfo
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- CN103300073B CN103300073B CN201310212388.7A CN201310212388A CN103300073B CN 103300073 B CN103300073 B CN 103300073B CN 201310212388 A CN201310212388 A CN 201310212388A CN 103300073 B CN103300073 B CN 103300073B
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Abstract
The invention discloses cannabis sativa antibacterial liquid as well as a preparation method and an application of the cannabis sativa antibacterial liquid. The cannabis sativa antibacterial liquid is prepared from cannabis sativa flowers and leaves through pre-treatment, extraction, condensation, filling and other steps, wherein the cannabis sativa antibacterial liquid is 8-12% in concentration. The preparation method comprises the steps of pre-treatment, extraction, condensation and filling, specifically comprises the steps of carefully selecting, cleaning and drying cannabis sativa flowers and leaves for further use; storing the pre-treated raw material cannabis sativa flowers and leaves in an extracting tank, adding water with the weight being 6-20 times of that of the raw material cannabis sativa flowers and leaves, and extracting for 6-10 hours at 98-100 DEG C; condensing extracting liquid under pressure of 0.8Mpa at temperature being lower than 90 DEG C to obtain a water solution of which the concentration is 8-12%, thus obtaining a target product; and filling the target product which is inspected to be qualified. The application means an application of the cannabis sativa antibacterial liquid in preparation of antibacterial articles for everyday use. The cannabis sativa antibacterial liquid, which is free of an organic reagent, is natural and non-toxic, simple in process, friendly to the environment and low in cost. And detection shows that the antibacterial liquid prepared by the extraction method is lower than 30ppm in THC content, thus being favorable for popularization of industrial production.
Description
Technical field
The invention belongs to technical field of plant extraction, be specifically related to a kind of hemp antibacterial liquid and preparation method thereof and application.
Background technology
Hemp, annual herb, high 1-3 rice.Root is wooden.Stem is upright, and tool longitudinal furrow, celadon, close by pubescence, cortex is rich in fiber.The leaf of palmately compound leaf alternate or bottom is to life, leaflet, lanceolar to strip lanceolar, long 7-15 centimetre, tip is gradually sharp, and base portion is gradually narrow, edge tool rough sawn tooth, above bottle green, by undercoat, below light green, close by canescence felt hair; The long 4-14 centimetre of petiole, close by short drafts hair; Stipule strip lanceolar, close by short drafts hair.Flower unisexuality, dioecism; Male flower yellow green, the panicle that arrangement is grown up and evacuated, tepal 5, long avette, stamen 5; The short spike of female inflorescence, is born in axil, and green, often spend the avette bract of outer tool l, tepal 1, film quality, hard-pressed bale ovary, gynoecium 1, ovary is upper, spherical, style two discrimination.The flat oval of achene, smooth and have fine-structure mesh line, the yellowish-brown bract deposited by place is wrapped up.The florescence 6-8 month, the fruit phase 8-10 month.
Industrial hemp is the low toxicity Hemp Varieties obtained by the seed selection of breeding man; its toxic component tetrahydrocannabinol (THC) is less than 0.3%; do not possess drugs value, as the raw material specializing in industrial use, can only can carry out large-scale planting and industrialized utilization.Industrial hemp is the traditional economic crops of China, is again the economic crops with outstanding versatility simultaneously.
Report according to domestic and international related documents, bacteriostatic active ingredients in hemp mostly is cannabinoids, oleanolic acid, oleanene, flavonoids etc., there is obvious malicious inhibitory action to staphylococcus aureus, bacillus subtilis and Candida albicans etc., and to 25 kinds of fungies such as aspergillus, Penicillium, Cladosporiums, there is bacteriostasis.
The Solvent Extract methods such as the multiplex benzinum of extracting method, chloroform, ethanol of bacteriostatic active ingredients in the industrial hemp floral leaf that document is recorded, there is organic reagent extraction consumption large, solvent recovery loss is larger, the shortcoming that extraction process is loaded down with trivial details, extract Poisoning composition THC has the trend of orienting enriching simultaneously, is unfavorable for the popularization of suitability for industrialized production.For solving appeal problem, develop a kind of method easy, reduce toxic component, reduce production cost, the extracting method of stay-in-grade hemp bacteriostatic active ingredients is very important.
Summary of the invention
The first object of the present invention is to provide a kind of hemp antibacterial liquid; Second object is the preparation method providing described hemp antibacterial liquid; 3rd object is to provide the application of described hemp antibacterial liquid in the antibacterial commodity of preparation.
The first object of the present invention is achieved in that described hemp antibacterial liquid concentration is 8 ~ 12% with hemp floral leaf for raw material obtains through pre-treatment, extraction, concentrated, filling step.
The second object of the present invention is achieved in that and comprises pre-treatment, extraction, concentrated, filling step, specifically comprises:
A, pre-treatment: hemp floral leaf is carried out selected, clean, dry for subsequent use;
B, extraction: pretreated raw material hemp floral leaf is inserted extractor, adds the water of weight ratio 6 ~ 20 times, extract 6 ~ 10h at 98 ~ 100 DEG C;
C, concentrated: by extract at below pressure 0.8Mpa, temperature less than 90 DEG C carry out being concentrated into concentration be 8 ~ 12% the aqueous solution obtain object;
D, filling: to carry out filling after the assay was approved.
The third object of the present invention is achieved in that the application of described hemp antibacterial liquid in the antibacterial commodity of preparation.
The present invention does not use organic reagent, Nantural non-toxic, and technique is simple, and environmental protection is with low cost.After testing, THC content in the antibacterial liquid of this extracting method is used lower than 30ppm, to be beneficial to the popularization of suitability for industrialized production.After testing, the compound such as soluble derivative, flavonoids containing cannabinoids in this antibacterial liquid, there is significant inhibitory action (P < 0.001) equally to staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans, can be used as the raw material of the household chemicalss such as bacteriostatic hand sanitizer.
Accompanying drawing explanation
Fig. 1 is present invention process flow chart.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further illustrated, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or replacement, all belong to protection scope of the present invention.
Hemp antibacterial liquid of the present invention is that described hemp antibacterial liquid concentration is 8 ~ 12% with hemp floral leaf for raw material obtains through pre-treatment, extraction, concentrated, filling step.
Described raw material is industrial hemp floral leaf.
Described raw material is the industrial hemp floral leaf of fiber plantation.
Described hemp antibacterial liquid is concentrating agents.
Described hemp antibacterial liquid is spray.
The preparation method of hemp antibacterial liquid of the present invention, comprises pre-treatment, extraction, concentrated, filling step, specifically comprises:
A, pre-treatment: hemp floral leaf is carried out selected, clean, dry for subsequent use;
B, extraction: pretreated raw material hemp floral leaf is inserted extractor, adds the water of weight ratio 6 ~ 20 times, extract 6 ~ 10h at 98 ~ 100 DEG C;
C, concentrated: by extract at below pressure 0.8Mpa, temperature less than 90 DEG C carry out being concentrated into concentration be 8 ~ 12% the aqueous solution obtain object;
D, filling: to carry out filling after the assay was approved.
Described cleaning carries out rinsing for using circulating water.
Described water is purified water or distilled water.
Described concentrated be at below pressure 0.8Mpa, carry out below temperature 50 C being concentrated into the aqueous solution that concentration is 10%.
Of the present invention be applied as described hemp antibacterial liquid preparation antibacterial commodity in application.
Extraction process of the present invention does not use organic reagent, Nantural non-toxic, and technique is simple, and environmental protection is with low cost.After testing, THC content in the antibacterial liquid of this extracting method is used lower than 30ppm, to be beneficial to the popularization of suitability for industrialized production.
After testing, the compound such as soluble derivative, flavonoids containing cannabinoids in this antibacterial liquid, there is significant inhibitory action (P < 0.001) equally to staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans, can be used as the raw material of the household chemicalss such as bacteriostatic hand sanitizer.
Embodiment 1
10Kg hemp floral leaf is carried out selected, clean, dry; Insert extractor, add the water of weight ratio 6 times, at 98 DEG C, extract 10h; By extract at pressure 0.7Mpa, temperature 90 DEG C carry out being concentrated into concentration be 8% the aqueous solution obtain antibacterial liquid, carry out filling after the assay was approved.After testing, wherein THC content is 10ppm.
Embodiment 2
10Kg hemp floral leaf is carried out selected, clean, dry; Insert extractor, add the water of weight ratio 20 times, at 100 DEG C, extract 6h; By extract at pressure 0.8Mpa, temperature 50 C carry out being concentrated into concentration be 12% the aqueous solution obtain antibacterial liquid, carry out filling after the assay was approved.After testing, wherein THC content is 12ppm.
Embodiment 3
10Kg hemp floral leaf is carried out selected, clean, dry; Insert extractor, add the water of weight ratio 15 times, at 99 DEG C, extract 8h; By extract at pressure 0.5Mpa, temperature 70 C carry out being concentrated into concentration be 10% the aqueous solution obtain antibacterial liquid, carry out filling after the assay was approved.After testing, wherein THC content is 8ppm.
Embodiment 4
10Kg hemp floral leaf is carried out selected, clean, dry; Insert extractor, add the water of weight ratio 10 times, at 100 DEG C, extract 9h; By extract at pressure 0.6Mpa, temperature 30 DEG C carry out being concentrated into concentration be 11% the aqueous solution obtain antibacterial liquid, carry out filling after the assay was approved.After testing, wherein THC content is 13ppm.
Embodiment 5
10Kg hemp floral leaf is carried out selected, clean, dry; Insert extractor, add the water of weight ratio 6 ~ 20 times, at 100 DEG C, extract 8h; By extract at pressure 0.5Mpa, temperature 60 C carry out being concentrated into concentration be 10% the aqueous solution obtain antibacterial liquid, carry out filling after the assay was approved.After testing, wherein THC content is 5ppm.
Claims (8)
1. the hemp antibacterial liquid preparation method that THC content is low, it is characterized in that described hemp antibacterial liquid concentration is 8 ~ 12%, and preparation method specifically comprises with industrial hemp floral leaf for raw material obtains through pre-treatment, extraction, concentrated, filling step:
A, pre-treatment: hemp floral leaf is carried out selected, clean, dry for subsequent use;
B, extraction: pretreated raw material hemp floral leaf is inserted extractor, adds the water of weight ratio 6 ~ 20 times, extract 6 ~ 10h at 98 ~ 100 DEG C;
C, concentrated: by extract at below pressure 0.8MPa, temperature less than 90 DEG C carry out being concentrated into concentration be 8 ~ 12% the aqueous solution obtain the object that THC content is less than 30ppm, have remarkable inhibitory action to staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans;
D, filling: to carry out filling after the assay was approved.
2. hemp antibacterial liquid preparation method according to claim 1, is characterized in that described raw material is the industrial hemp floral leaf of fiber plantation.
3. hemp antibacterial liquid preparation method according to claim 1, is characterized in that described hemp antibacterial liquid is concentrating agents.
4. hemp antibacterial liquid preparation method according to claim 1, is characterized in that described hemp antibacterial liquid is spray.
5. hemp antibacterial liquid preparation method according to claim 1, is characterized in that described cleaning carries out rinsing for using circulating water.
6. hemp antibacterial liquid preparation method according to claim 1, is characterized in that described water is purified water or distilled water.
7. hemp antibacterial liquid preparation method according to claim 1, is characterized in that the THC content of described object is 5,8,10,12 or 13ppm.
8. hemp antibacterial liquid preparation method according to claim 1, it is characterized in that described concentrated be at below pressure 0.8MPa, carry out below temperature 50 C being concentrated into the aqueous solution that concentration is 10%.
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107158083B (en) * | 2016-03-08 | 2021-05-11 | 中国人民解放军总后勤部军需装备研究所 | Hemp leaf extract and application thereof in preparation of anti-fatigue product |
| CN107802530B (en) * | 2016-09-06 | 2021-04-13 | 汉义生物科技（北京）有限公司 | Composition containing cannabis extract and application of composition in cosmetics |
| CN106265364A (en) * | 2016-09-30 | 2017-01-04 | 汉义生物科技（北京）有限公司 | A kind of compositions containing Fructus Cannabis extract and the application in cleaning supplies thereof |
| US10239808B1 (en) | 2016-12-07 | 2019-03-26 | Canopy Holdings, LLC | Cannabis extracts |
| CN108451799A (en) * | 2016-12-09 | 2018-08-28 | 汉义生物科技（北京）有限公司 | A kind of hand cleanser and preparation method thereof |
| CN108203631A (en) * | 2016-12-16 | 2018-06-26 | 汉义生物科技（北京）有限公司 | A kind of antibacterial cleaning products and preparation method thereof |
| CN107324852A (en) * | 2017-07-26 | 2017-11-07 | 云南汉木森生物科技有限责任公司 | A kind of soil additive planted for pollution-free food and its application method |
| US11202771B2 (en) | 2018-01-31 | 2021-12-21 | Treehouse Biotech, Inc. | Hemp powder |
| WO2020077153A1 (en) | 2018-10-10 | 2020-04-16 | Canopy Holdings, LLC | Synthesis of cannabigerol |
| CN111378539A (en) * | 2018-12-28 | 2020-07-07 | 黑龙江惠美佳制药有限公司 | Handmade soap containing cannabis polyphenol extract |
| WO2023039421A1 (en) * | 2021-09-07 | 2023-03-16 | Demetrix, Inc. | Use of cannabinoid compounds to treat or prevent fungal growth of c. albicans |
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Effective date of registration: 20160302 Address after: 100000, Beijing, Chaoyang District Jia Lou (Jing Guang Center) No. 1, building 13, room 26 Patentee after: Han Yi Biotechnology (Beijing) Co., Ltd. Address before: 650032, 4, Dongfeng Cang Road, Dongfeng West Road, Yunnan, Kunming Patentee before: Yunnan Kunhua Industry and Trade General Co., Ltd. |